HG10011604 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10011604
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	valencene synthase-like
Location	Chr01: 7914983 .. 7915253 (-)
RNA-Seq Expression	HG10011604
Synteny	HG10011604

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGTGGCTTCAGTTACCTCAGGTTATGCACTACTTGCAACTATTTCATTTGTTTGCATGGGTGACATTGCAACAAAGGAGGTATTTGATTGGCTTTTAGAATGCCCTAAAACTCTTGAAGCCTCAACCATCATTTGTAGACTCATGGACGATGTTGTCTCCTGCAAGGTATGTGTCACTCTCACTATCTAAATAAGTTCAGGACAAGATTGAAATATCTACCAATATCGTGATATATTCGTCGAATATCTATAAGTCAGAGAATTAA

mRNA sequence

ATGGAAGTGGCTTCAGTTACCTCAGGTTATGCACTACTTGCAACTATTTCATTTGTTTGCATGGGTGACATTGCAACAAAGGAGGTATTTGATTGGCTTTTAGAATGCCCTAAAACTCTTGAAGCCTCAACCATCATTTGTAGACTCATGGACGATGTTGTCTCCTGCAAGGACAAGATTGAAATATCTACCAATATCGTGATATATTCGTCGAATATCTATAAGTCAGAGAATTAA

Coding sequence (CDS)

ATGGAAGTGGCTTCAGTTACCTCAGGTTATGCACTACTTGCAACTATTTCATTTGTTTGCATGGGTGACATTGCAACAAAGGAGGTATTTGATTGGCTTTTAGAATGCCCTAAAACTCTTGAAGCCTCAACCATCATTTGTAGACTCATGGACGATGTTGTCTCCTGCAAGGACAAGATTGAAATATCTACCAATATCGTGATATATTCGTCGAATATCTATAAGTCAGAGAATTAA

Protein sequence

MEVASVTSGYALLATISFVCMGDIATKEVFDWLLECPKTLEASTIICRLMDDVVSCKDKIEISTNIVIYSSNIYKSEN

Homology

[Close]

BLAST of HG10011604 vs. NCBI nr
Match: XP_038903601.1 ((+)-gamma-cadinene synthase-like [Benincasa hispida])

HSP 1 Score: 95.9 bits (237), Expect = 1.6e-16
Identity = 46/55 (83.64%), Postives = 51/55 (92.73%), Query Frame = 0


Query: 1   MEVASVTSGYALLATISFVCMGDIATKEVFDWLLECPKTLEASTIICRLMDDVVS 56
           MEVASV+SGYALLATISF CMGD+AT+EVFDW+ ECPK LEASTII RLMDD+VS
Sbjct: 399 MEVASVSSGYALLATISFACMGDVATQEVFDWVFECPKILEASTIIARLMDDLVS 453

[Close]

BLAST of HG10011604 vs. NCBI nr
Match: KAE8650158.1 (hypothetical protein Csa_009547 [Cucumis sativus])

HSP 1 Score: 90.9 bits (224), Expect = 5.3e-15
Identity = 50/81 (61.73%), Postives = 61/81 (75.31%), Query Frame = 0


Query: 1   MEVASVTSGYALLATISFVCMGDIATKEVFDWLLECPKTLEASTIICRLMDDVVSC---K 60
           MEVA  +SGY LL+TISFVCMGDIATKEVF+WL +CPK L+AST I RLMDDVVS    K
Sbjct: 414 MEVALASSGYELLSTISFVCMGDIATKEVFEWLFDCPKILKASTTISRLMDDVVSYKFEK 473

Query: 61  DKIEISTNIVIYSSNIYKSEN 79
           ++  I + +  Y SN  +SE+
Sbjct: 474 EREHIVSAVECYMSNHGRSED 494

[Close]

BLAST of HG10011604 vs. NCBI nr
Match: XP_008452328.1 (PREDICTED: valencene synthase-like [Cucumis melo])

HSP 1 Score: 90.5 bits (223), Expect = 6.9e-15
Identity = 45/57 (78.95%), Postives = 49/57 (85.96%), Query Frame = 0


Query: 1  MEVASVTSGYALLATISFVCMGDIATKEVFDWLLECPKTLEASTIICRLMDDVVSCK 58
          MEVA  +SGY LL+TISFVCMGDIATKEVFDWL +CPK L+AST I RLMDDVVS K
Sbjct: 1  MEVALASSGYELLSTISFVCMGDIATKEVFDWLFDCPKILKASTTISRLMDDVVSYK 57

[Close]

BLAST of HG10011604 vs. NCBI nr
Match: XP_038905757.1 ((+)-gamma-cadinene synthase-like [Benincasa hispida])

HSP 1 Score: 90.1 bits (222), Expect = 9.0e-15
Identity = 46/57 (80.70%), Postives = 48/57 (84.21%), Query Frame = 0


Query: 1   MEVASVTSGYALLATISFVCMGDIATKEVFDWLLECPKTLEASTIICRLMDDVVSCK 58
           MEVA V+SGY LL TISF CMGDIATKEVFDWL ECPK LEASTII RL +DVVS K
Sbjct: 411 MEVALVSSGYGLLTTISFACMGDIATKEVFDWLSECPKILEASTIISRLTNDVVSYK 467

[Close]

BLAST of HG10011604 vs. NCBI nr
Match: NP_001292628.1 ((+)-gamma-cadinene synthase [Cucumis sativus] >AAU05952.1 beta-caryophyllene synthase [Cucumis sativus])

HSP 1 Score: 89.4 bits (220), Expect = 1.5e-14
Identity = 49/81 (60.49%), Postives = 61/81 (75.31%), Query Frame = 0


Query: 1   MEVASVTSGYALLATISFVCMGDIATKEVFDWLLECPKTLEASTIICRLMDDVVSC---K 60
           MEVA  +SGY LL+TISFVCMGDIATKEVF+WL +CP+ L+AST I RLMDDVVS    K
Sbjct: 414 MEVALASSGYELLSTISFVCMGDIATKEVFEWLFDCPQILKASTTISRLMDDVVSYKFEK 473

Query: 61  DKIEISTNIVIYSSNIYKSEN 79
           ++  I + +  Y SN  +SE+
Sbjct: 474 EREHIVSAVECYMSNHGRSED 494

[Close]

BLAST of HG10011604 vs. ExPASy Swiss-Prot
Match: Q6Q3H3 ((-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 1.3e-11
Identity = 34/54 (62.96%), Postives = 40/54 (74.07%), Query Frame = 0


Query: 1   MEVASVTSGYALLATISFVCMGDIATKEVFDWLLECPKTLEASTIICRLMDDVV 55
           M VA VTS Y++LAT SFV MGD  TKE FDW+   PK + AS I+CRLMDD+V
Sbjct: 404 MPVALVTSAYSMLATTSFVGMGDAVTKESFDWIFSKPKIVRASAIVCRLMDDMV 457

[Close]

BLAST of HG10011604 vs. ExPASy Swiss-Prot
Match: B5A435 (Sesquiterpene synthase OS=Santalum album OX=35974 PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 4.8e-11
Identity = 34/57 (59.65%), Postives = 41/57 (71.93%), Query Frame = 0


Query: 1   MEVASVTSGYALLATISFVCMGDIATKEVFDWLLECPKTLEASTIICRLMDDVVSCK 58
           M VA VTSGY  L TIS++ MG+IA+KE FDWL   P  +EAS  +CRLMDD+ S K
Sbjct: 406 MPVALVTSGYTFLTTISYLGMGEIASKEAFDWLFSHPPVIEASESVCRLMDDMRSHK 462

[Close]

BLAST of HG10011604 vs. ExPASy Swiss-Prot
Match: B9SCB6 (Probable terpene synthase 2 OS=Ricinus communis OX=3988 GN=TPS2 PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 8.2e-11
Identity = 33/55 (60.00%), Postives = 39/55 (70.91%), Query Frame = 0


Query: 1   MEVASVTSGYALLATISFVCMGDIATKEVFDWLLECPKTLEASTIICRLMDDVVS 56
           M +A V+  Y LL   SFV MGDIATKEVFDW    PK +  ++IICRLMDD+VS
Sbjct: 399 MSIALVSCAYPLLTVTSFVGMGDIATKEVFDWASNDPKIVRVASIICRLMDDIVS 453

[Close]

BLAST of HG10011604 vs. ExPASy Swiss-Prot
Match: F6M8H6 (Probable sesquiterpene synthase OS=Santalum spicatum OX=453088 GN=SesquiTPS PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.4e-10
Identity = 32/55 (58.18%), Postives = 40/55 (72.73%), Query Frame = 0


Query: 1   MEVASVTSGYALLATISFVCMGDIATKEVFDWLLECPKTLEASTIICRLMDDVVS 56
           M VA VT+GY  L TIS++ MG+IA+KE FDWL   P  +EAS  +CRLMDD+ S
Sbjct: 409 MSVALVTAGYTFLTTISYLGMGEIASKEAFDWLFSRPPIIEASESVCRLMDDMRS 463

[Close]

BLAST of HG10011604 vs. ExPASy Swiss-Prot
Match: Q6Q3H2 (Valencene synthase OS=Vitis vinifera OX=29760 GN=ValCS PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.4e-10
Identity = 34/57 (59.65%), Postives = 40/57 (70.18%), Query Frame = 0


Query: 1   MEVASVTSGYALLATISFVCMGDIATKEVFDWLLECPKTLEASTIICRLMDDVVSCK 58
           M VA  +SGY LLAT SFV MG+IATKE FDW+   PK + +S  I RLMDD+ S K
Sbjct: 404 MRVALASSGYCLLATTSFVGMGEIATKEAFDWVTSDPKIMSSSNFITRLMDDIKSHK 460

[Close]

BLAST of HG10011604 vs. ExPASy TrEMBL
Match: A0A0A0L579 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G097040 PE=4 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 2.6e-15
Identity = 50/81 (61.73%), Postives = 61/81 (75.31%), Query Frame = 0


Query: 1   MEVASVTSGYALLATISFVCMGDIATKEVFDWLLECPKTLEASTIICRLMDDVVSC---K 60
           MEVA  +SGY LL+TISFVCMGDIATKEVF+WL +CPK L+AST I RLMDDVVS    K
Sbjct: 414 MEVALASSGYELLSTISFVCMGDIATKEVFEWLFDCPKILKASTTISRLMDDVVSYKFEK 473

Query: 61  DKIEISTNIVIYSSNIYKSEN 79
           ++  I + +  Y SN  +SE+
Sbjct: 474 EREHIVSAVECYMSNHGRSED 494

[Close]

BLAST of HG10011604 vs. ExPASy TrEMBL
Match: A0A1S3BTM3 (valencene synthase-like OS=Cucumis melo OX=3656 GN=LOC103493387 PE=4 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 3.3e-15
Identity = 45/57 (78.95%), Postives = 49/57 (85.96%), Query Frame = 0


Query: 1  MEVASVTSGYALLATISFVCMGDIATKEVFDWLLECPKTLEASTIICRLMDDVVSCK 58
          MEVA  +SGY LL+TISFVCMGDIATKEVFDWL +CPK L+AST I RLMDDVVS K
Sbjct: 1  MEVALASSGYELLSTISFVCMGDIATKEVFDWLFDCPKILKASTTISRLMDDVVSYK 57

[Close]

BLAST of HG10011604 vs. ExPASy TrEMBL
Match: Q66PX8 (Beta-caryophyllene synthase OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 7.4e-15
Identity = 49/81 (60.49%), Postives = 61/81 (75.31%), Query Frame = 0


Query: 1   MEVASVTSGYALLATISFVCMGDIATKEVFDWLLECPKTLEASTIICRLMDDVVSC---K 60
           MEVA  +SGY LL+TISFVCMGDIATKEVF+WL +CP+ L+AST I RLMDDVVS    K
Sbjct: 414 MEVALASSGYELLSTISFVCMGDIATKEVFEWLFDCPQILKASTTISRLMDDVVSYKFEK 473

Query: 61  DKIEISTNIVIYSSNIYKSEN 79
           ++  I + +  Y SN  +SE+
Sbjct: 474 EREHIVSAVECYMSNHGRSED 494

[Close]

BLAST of HG10011604 vs. ExPASy TrEMBL
Match: A0A0B4VEV4 (Sesquiterpene synthase Tps1 OS=Cucumis melo OX=3656 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 7.4e-15
Identity = 48/80 (60.00%), Postives = 59/80 (73.75%), Query Frame = 0


Query: 1   MEVASVTSGYALLATISFVCMGDIATKEVFDWLLECPKTLEASTIICRLMDDVVSC---K 60
           MEVA  +SGY LL+TISFVCMGDIATKE+FDWL +CPK L+AST I RLMDDVVS    K
Sbjct: 417 MEVALASSGYELLSTISFVCMGDIATKEIFDWLFDCPKILKASTTISRLMDDVVSYKFEK 476

Query: 61  DKIEISTNIVIYSSNIYKSE 78
           ++  + + +  Y +N   SE
Sbjct: 477 EREHVVSAVECYMNNYGCSE 496

[Close]

BLAST of HG10011604 vs. ExPASy TrEMBL
Match: A0A1S4DZR2 ((+)-gamma-cadinene synthase-like OS=Cucumis melo OX=3656 GN=LOC103493390 PE=4 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 2.2e-14
Identity = 47/80 (58.75%), Postives = 59/80 (73.75%), Query Frame = 0


Query: 1   MEVASVTSGYALLATISFVCMGDIATKEVFDWLLECPKTLEASTIICRLMDDVVSC---K 60
           MEVA  +SGY LL+TISFVCMGDIAT+E+FDWL +CPK L+AST I RLMDDVVS    K
Sbjct: 417 MEVALASSGYELLSTISFVCMGDIATEEIFDWLFDCPKILKASTTISRLMDDVVSYKFEK 476

Query: 61  DKIEISTNIVIYSSNIYKSE 78
           ++  + + +  Y +N   SE
Sbjct: 477 EREHVVSAVECYMNNYGCSE 496

[Close]

BLAST of HG10011604 vs. TAIR 10
Match: AT5G48110.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 47.0 bits (110), Expect = 8.1e-06
Identity = 29/72 (40.28%), Postives = 41/72 (56.94%), Query Frame = 0


Query: 1   MEVASVTSGYALLATISFVCMGDIATKEVFDWLLECPKTLEASTIICRLMDDVVSCKDKI 60
           MEV  VT G  +    +F+ MG+ A KE FDW+   PK ++   +  RL DDV + KD++
Sbjct: 425 MEVGIVTGGVDVTVAFAFIGMGE-AGKEAFDWIRSRPKFIQTIDLKSRLRDDVATYKDEM 484

Query: 61  ---EISTNIVIY 70
              EI+T I  Y
Sbjct: 485 ARGEIATGINCY 495

[Close]

BLAST of HG10011604 vs. TAIR 10
Match: AT5G23960.1 (terpene synthase 21 )

HSP 1 Score: 44.3 bits (103), Expect = 5.3e-05
Identity = 24/54 (44.44%), Postives = 32/54 (59.26%), Query Frame = 0


Query: 2   EVASVTSGYALLATISFVCMGDIATKEVFDWLLECPKTLEASTIICRLMDDVVS 56
           E A ++SGY  L  ++FV M D+A  + F+WL   PK   AS II R  DD+ S
Sbjct: 396 ENAILSSGYYALIAMTFVRMTDVAKLDAFEWLSSHPKIRVASEIISRFTDDISS 449

[Close]

BLAST of HG10011604 vs. TAIR 10
Match: AT5G23960.2 (terpene synthase 21 )

HSP 1 Score: 44.3 bits (103), Expect = 5.3e-05
Identity = 24/54 (44.44%), Postives = 32/54 (59.26%), Query Frame = 0


Query: 2   EVASVTSGYALLATISFVCMGDIATKEVFDWLLECPKTLEASTIICRLMDDVVS 56
           E A ++SGY  L  ++FV M D+A  + F+WL   PK   AS II R  DD+ S
Sbjct: 394 ENAILSSGYYALIAMTFVRMTDVAKLDAFEWLSSHPKIRVASEIISRFTDDISS 447

[Close]

BLAST of HG10011604 vs. TAIR 10
Match: AT4G20200.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 42.4 bits (98), Expect = 2.0e-04
Identity = 31/78 (39.74%), Postives = 46/78 (58.97%), Query Frame = 0


Query: 1   MEVASV-TSGYALLATISFVCMGDIATKEVFDWLLECPKTLEASTIICRLMDDVVSCKDK 60
           MEV  V  + YA LA I F+ MG +ATKE F+WL   PK ++  +I  RLM+D++  +D 
Sbjct: 451 MEVGEVEVTVYASLAGI-FMSMGKMATKEAFEWLKSRPKLVQYLSIKGRLMNDLMGYED- 510

Query: 61  IEISTNIVIYSSNIYKSE 78
            ++S   V  + N Y  +
Sbjct: 511 -DMSRGYVTNAVNCYMKQ 525

[Close]

BLAST of HG10011604 vs. TAIR 10
Match: AT3G32030.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 42.0 bits (97), Expect = 2.6e-04
Identity = 25/78 (32.05%), Postives = 43/78 (55.13%), Query Frame = 0


Query: 1   MEVASVTSGYALLATISFVCMGDIATKEVFDWLLECPKTLEASTIICRLMDDVVSCKDKI 60
           MEV  VT G    A  SF+ M D   K +++W    PK L+A +++ R+ +D+V+ +   
Sbjct: 450 MEVGEVTGGMDDFALYSFIAMEDCDEKPLYEWFDSKPKILQALSVLYRINNDIVTYER-- 509

Query: 61  EISTNIVIYSSNIYKSEN 79
           E+S   V+   N Y +++
Sbjct: 510 EMSKGEVVNGVNSYMNQH 525

The following BLAST results are available for this feature:

BLAST of HG10011604 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038903601.1	1.6e-16	83.64	(+)-gamma-cadinene synthase-like [Benincasa hispida]	[more]
KAE8650158.1	5.3e-15	61.73	hypothetical protein Csa_009547 [Cucumis sativus]	[more]
XP_008452328.1	6.9e-15	78.95	PREDICTED: valencene synthase-like [Cucumis melo]	[more]
XP_038905757.1	9.0e-15	80.70	(+)-gamma-cadinene synthase-like [Benincasa hispida]	[more]
NP_001292628.1	1.5e-14	60.49	(+)-gamma-cadinene synthase [Cucumis sativus] >AAU05952.1 beta-caryophyllene syn...	[more]

BLAST of HG10011604 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6Q3H3	1.3e-11	62.96	(-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 S...	[more]
B5A435	4.8e-11	59.65	Sesquiterpene synthase OS=Santalum album OX=35974 PE=1 SV=1	[more]
B9SCB6	8.2e-11	60.00	Probable terpene synthase 2 OS=Ricinus communis OX=3988 GN=TPS2 PE=3 SV=1	[more]
F6M8H6	1.4e-10	58.18	Probable sesquiterpene synthase OS=Santalum spicatum OX=453088 GN=SesquiTPS PE=3...	[more]
Q6Q3H2	1.4e-10	59.65	Valencene synthase OS=Vitis vinifera OX=29760 GN=ValCS PE=1 SV=1	[more]

BLAST of HG10011604 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L579	2.6e-15	61.73	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G097040 PE=4 SV=1	[more]
A0A1S3BTM3	3.3e-15	78.95	valencene synthase-like OS=Cucumis melo OX=3656 GN=LOC103493387 PE=4 SV=1	[more]
Q66PX8	7.4e-15	60.49	Beta-caryophyllene synthase OS=Cucumis sativus OX=3659 PE=2 SV=1	[more]
A0A0B4VEV4	7.4e-15	60.00	Sesquiterpene synthase Tps1 OS=Cucumis melo OX=3656 PE=2 SV=1	[more]
A0A1S4DZR2	2.2e-14	58.75	(+)-gamma-cadinene synthase-like OS=Cucumis melo OX=3656 GN=LOC103493390 PE=4 SV...	[more]

BLAST of HG10011604 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G48110.1	8.1e-06	40.28	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]
AT5G23960.1	5.3e-05	44.44	terpene synthase 21	[more]
AT5G23960.2	5.3e-05	44.44	terpene synthase 21	[more]
AT4G20200.1	2.0e-04	39.74	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]
AT3G32030.1	2.6e-04	32.05	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005630	Terpene synthase, metal-binding domain	PFAM	PF03936	Terpene_synth_C	coord: 1..62 e-value: 4.2E-15 score: 55.8
IPR008949	Isoprenoid synthase domain superfamily	GENE3D	1.10.600.10	Farnesyl Diphosphate Synthase	coord: 1..78 e-value: 4.4E-16 score: 60.6
IPR008949	Isoprenoid synthase domain superfamily	SUPERFAMILY	48576	Terpenoid synthases	coord: 1..58
None	No IPR available	PANTHER	PTHR31225:SF134	(-)-GERMACRENE D SYNTHASE-LIKE	coord: 1..59
None	No IPR available	PANTHER	PTHR31225	OS04G0344100 PROTEIN-RELATED	coord: 1..59

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10011604.1	HG10011604.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016102	diterpenoid biosynthetic process
molecular_function	GO:0047461	(+)-delta-cadinene synthase activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0010333	terpene synthase activity