HG10011583 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10011583
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Gibberellin 2-beta-dioxygenase 8-like
Location	Chr01: 7462966 .. 7465657 (+)
RNA-Seq Expression	HG10011583
Synteny	HG10011583

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGGAGTGGTGAAGAAATTAGAGAGATTAGCAGAGAAGATATCAGAGATATTAGGGCAAAGTTTGGGGATAAAATCAAGTTACTTCAAAGAAAGATGTGAGAAAGGAAAGAGTAGCTTTCGACTGAATAGATATCCACCTTGTCCATTTGCTTCTAAAGTCTATGGTCTCATTCCTCACACCGACAGTGATTATCTTACAATACTTTACCAACCTCAAATCAGTGGTTTGCAACTCATGAAAGCTGGAAAATGGTTCCCTGTTAAACCTAACTCACAAGCTTTGCTTGTCAACATCGGTGACTTATTTCAGGTACCACTTCTTTTTTAACTTTCATCTCTTCATTTTCATTATTTCTCTTTTACTTTTCCATATTTATCATCTATAAATATCTTAATATATAAGAATCAAATGTAATCGGTTTTAGTAGTACTTTAATTACCCTAATCATTTCGTTTTTAGCTTTCTCTTTCTGAAATTTATAAATTTATGTATGTTTTCTCAGAAATATTTTAATTTCTAGTTTAATTCTAAAAATAAAAACAAAATTTTGAATTTTTTTATTTTTTATTTTTTATTTTTTAGTTTTCAAAATTTAATTAGATAAAAAAAAATATAGAAATTTATGGATGGAAATGGTGTTTATAATAAGCTTAATTTTTAAAAATTAAAAACTAAAAATAAAATGGTTATCGAATGAGATCTTAGTTTTTTTTATTTTAATTTTTAAAAACTACTTTTTCTTTTAGTAGTTTCCAAAAGGTCAAATTGCAATTTTAGTTCCAATGGTTTTGGAAAAAGTTAGAATTTAATCCCTATGGTTTCAAAAATTAGAATTTAGTCCTTGTCGTTTGATAAAATCTCCCTAAATAGTCTCTTTAGTAGGGACTATATATGAGTATATTATCAAATCATATGAACTATTTATGAGGTTTTATCAAACATAAATTCTAACTTTTTCAAAATCATAAAGACTAAATTTGTAATTAAACCTTTCAAAAACTTGCCCTGATTTTTTAAAATATTTAAAAAAAAAGTAGATAATAAAATAAAGATTGAAATCAATAGTGTTTATATAAACCTAAAAACTAAAAACAAAATAGAAAAGTTGTTTTTAAAAGAAATTATTTTAGAACAATCAACTAAAATTTAAAAGTTTGGAGAAAAAAAAAAGAGTAGATTTAAGACCTCAAAATATTTATTTAAAAATGTTAAAATAAAACAAACAACATATTGGTAACAAACATTAATTTTTAAAGCCAAAATCAGTATCAATAAGGTCATATTTTATAAAAGTTTGCTTTTCCCTTAAAAATACTATTAAATTTTTAATAAGAAACAAAAAAAATTATTGTTAAAAGTAGGAAAATAAACTTTGGACAGATTGGACAATGATTTAATATCTTTTGAAATTTCAGTTTTTATAAAACTGTCTTTGTTTTATTCAACAATTTTCTTTTTTAACTATCAGCTGTTAAAGATACTTTAAATATCTCACCAAAATTTCAAAATCCAAAAGAATATATTATTATAATAATAAGGGATATAATTACAAACTTTTTTTTTTTTTTTATCAGAAATTGAACATGTAGACTTTTAGATAGTTTGTTGTCAATTCCTTAGCTACTTGAATTATACTCCATTTAATATTTACCCTATATGCATGGACTTTTGAAAGATTTTGGGAATAGAGGGGTATTAAAAACCATTTTTTTAGAGAAATTACATGGCATTTGGAAAAAAAAAACTTTCTAAGTGAAAAAAAATAATGATTTGAAAAACAAAATTAGTTTTAAGAAACAGAAGACTAATATTAACAAAATAATTTTGTTATAGATTCCAAAATGTCTTTGGAGCCCTACACTTGTCATTGCTAAAATTTATAGGGTAATTATAATGAATAGCATTTAGAGATAATAACCAAGTATACAGTAATATTTAAAAAAAATTACAAATATAGCAAAGTCTATCAGCGATAGACTCTTATGGTCTATCCGTGATAGACCACCTAGATCATAAAAGTCTAATAAACAATAAAAGTCTATCATTGATAGACTTTGCTATATTTACAATTTTTCAAAATTGTTACTATATATTATACTTTAAATTTGATTGTTATATTTGCAACTGTACCAAACTCATATCTTTTTGGGTACTTTCTACTTGCATAAATTAGATAGTTAGATGAAATAACAATTACGTACACTTGAAAATGGAAAGACTTAAACATCATATATATATATATATATATATTTGAAATTAAGAACTAAATTTCCAAATGGTAACCTTAAAGATTGCTTCTTCTCAAGAATAATATATGGGAAAAGAATCAAAAGGACATGTAAAGAGAACATAATTAACATGTATATATATATATATAAATTACTAACCTTTATTTGTGAAGAGAGTGATCTTAAATTGGTAAAAAAATTGAATAATATTGTGATGGCAGGTTGTGAGCAATGATGTTTTTAGGAGCCTTAAACACAGAGTGGTTGCATCTGAAGAAGTTGAAAGATTTTCCTTTGCATATTTCTATTGCCCATCTGATGATGTGATGATTGAGAGTTGGTTGAAACCAAGCATTTACAGGCAATTCAGCTACAAAGAGTATAGACAACAAATAGAAAAAGATGTAGAGAAAACAGGCAACAAAGTAGGGCTTTCAAGGTTTTTTCTTGCACAATAA

mRNA sequence

ATGGAAGGAGTGGTGAAGAAATTAGAGAGATTAGCAGAGAAGATATCAGAGATATTAGGGCAAAGTTTGGGGATAAAATCAAGTTACTTCAAAGAAAGATGTGAGAAAGGAAAGAGTAGCTTTCGACTGAATAGATATCCACCTTGTCCATTTGCTTCTAAAGTCTATGGTCTCATTCCTCACACCGACAGTGATTATCTTACAATACTTTACCAACCTCAAATCAGTGGTTTGCAACTCATGAAAGCTGGAAAATGGTTCCCTGTTAAACCTAACTCACAAGCTTTGCTTGTCAACATCGGTGACTTATTTCAGGTTGTGAGCAATGATGTTTTTAGGAGCCTTAAACACAGAGTGGTTGCATCTGAAGAAGTTGAAAGATTTTCCTTTGCATATTTCTATTGCCCATCTGATGATGTGATGATTGAGAGTTGGTTGAAACCAAGCATTTACAGGCAATTCAGCTACAAAGAGTATAGACAACAAATAGAAAAAGATGTAGAGAAAACAGGCAACAAAGTAGGGCTTTCAAGGTTTTTTCTTGCACAATAA

Coding sequence (CDS)

ATGGAAGGAGTGGTGAAGAAATTAGAGAGATTAGCAGAGAAGATATCAGAGATATTAGGGCAAAGTTTGGGGATAAAATCAAGTTACTTCAAAGAAAGATGTGAGAAAGGAAAGAGTAGCTTTCGACTGAATAGATATCCACCTTGTCCATTTGCTTCTAAAGTCTATGGTCTCATTCCTCACACCGACAGTGATTATCTTACAATACTTTACCAACCTCAAATCAGTGGTTTGCAACTCATGAAAGCTGGAAAATGGTTCCCTGTTAAACCTAACTCACAAGCTTTGCTTGTCAACATCGGTGACTTATTTCAGGTTGTGAGCAATGATGTTTTTAGGAGCCTTAAACACAGAGTGGTTGCATCTGAAGAAGTTGAAAGATTTTCCTTTGCATATTTCTATTGCCCATCTGATGATGTGATGATTGAGAGTTGGTTGAAACCAAGCATTTACAGGCAATTCAGCTACAAAGAGTATAGACAACAAATAGAAAAAGATGTAGAGAAAACAGGCAACAAAGTAGGGCTTTCAAGGTTTTTTCTTGCACAATAA

Protein sequence

MEGVVKKLERLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIPHTDSDYLTILYQPQISGLQLMKAGKWFPVKPNSQALLVNIGDLFQVVSNDVFRSLKHRVVASEEVERFSFAYFYCPSDDVMIESWLKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLSRFFLAQ

Homology

[Close]

BLAST of HG10011583 vs. NCBI nr
Match: XP_038906005.1 (gibberellin 2-beta-dioxygenase 8-like [Benincasa hispida])

HSP 1 Score: 355.5 bits (911), Expect = 2.7e-94
Identity = 174/181 (96.13%), Postives = 175/181 (96.69%), Query Frame = 0


Query: 1   MEGVVKKLERLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           MEGVVKK  RLAE ISE+LGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP
Sbjct: 162 MEGVVKKFGRLAENISEVLGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 221

Query: 61  HTDSDYLTILYQPQISGLQLMKAGKWFPVKPNSQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTDSDYLTILYQPQ SGLQLMKAGKWFPVKPN QALLVNIGDLFQVVSNDVFRSLKHRVV
Sbjct: 222 HTDSDYLTILYQPQTSGLQLMKAGKWFPVKPNPQALLVNIGDLFQVVSNDVFRSLKHRVV 281

Query: 121 ASEEVERFSFAYFYCPSDDVMIESWLKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180
           ASE VERFSFAYFYCPSDDVMIESWLKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF
Sbjct: 282 ASEGVERFSFAYFYCPSDDVMIESWLKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF 341

Query: 181 L 182
           L
Sbjct: 342 L 342

[Close]

BLAST of HG10011583 vs. NCBI nr
Match: TYK04745.1 (gibberellin 2-beta-dioxygenase 8-like [Cucumis melo var. makuwa])

HSP 1 Score: 340.1 bits (871), Expect = 1.2e-89
Identity = 164/181 (90.61%), Postives = 173/181 (95.58%), Query Frame = 0


Query: 1   MEGVVKKLERLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           MEGVVKKL  LAEKISEILGQSLGIKS+YFKERCEKGKSSFR+NRYPPCP AS+VYGLIP
Sbjct: 1   MEGVVKKLGSLAEKISEILGQSLGIKSNYFKERCEKGKSSFRMNRYPPCPIASQVYGLIP 60

Query: 61  HTDSDYLTILYQPQISGLQLMKAGKWFPVKPNSQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTD+DYLTILYQP ISGLQLMK+ KWFPVKPN QALL+NIGDLFQVVSND+FRSLKHRVV
Sbjct: 61  HTDTDYLTILYQPHISGLQLMKSAKWFPVKPNPQALLLNIGDLFQVVSNDIFRSLKHRVV 120

Query: 121 ASEEVERFSFAYFYCPSDDVMIESWLKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180
           AS  VER+SFAYFYCPSDDVMIESWLKPSIY+QFSYKEYRQQIEKDVEKTGNKVGLSRFF
Sbjct: 121 ASVGVERYSFAYFYCPSDDVMIESWLKPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180

Query: 181 L 182
           L
Sbjct: 181 L 181

[Close]

BLAST of HG10011583 vs. NCBI nr
Match: XP_004152933.2 (gibberellin 2-beta-dioxygenase 8 [Cucumis sativus] >XP_031737838.1 gibberellin 2-beta-dioxygenase 8 [Cucumis sativus] >KAE8650147.1 hypothetical protein Csa_009716 [Cucumis sativus])

HSP 1 Score: 337.4 bits (864), Expect = 7.6e-89
Identity = 162/181 (89.50%), Postives = 173/181 (95.58%), Query Frame = 0


Query: 1   MEGVVKKLERLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           MEGVVKKL  LAEKISEILGQSLGIKS+YFKERCEKGKSSFR+NRYPPCP AS+VYGLIP
Sbjct: 157 MEGVVKKLGSLAEKISEILGQSLGIKSNYFKERCEKGKSSFRMNRYPPCPIASQVYGLIP 216

Query: 61  HTDSDYLTILYQPQISGLQLMKAGKWFPVKPNSQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTD+DYLTILYQPQISGLQL K+ KWFPVKPN +ALL+NIGDLFQVVSND+FRSLKHRVV
Sbjct: 217 HTDTDYLTILYQPQISGLQLKKSAKWFPVKPNPRALLLNIGDLFQVVSNDIFRSLKHRVV 276

Query: 121 ASEEVERFSFAYFYCPSDDVMIESWLKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180
           ASE VER+SFAYFYCPSDDV+IESWLKPSIY+ FSYKEYRQQIEKDVEKTGNKVGLSRFF
Sbjct: 277 ASEGVERYSFAYFYCPSDDVIIESWLKPSIYKHFSYKEYRQQIEKDVEKTGNKVGLSRFF 336

Query: 181 L 182
           L
Sbjct: 337 L 337

[Close]

BLAST of HG10011583 vs. NCBI nr
Match: XP_008463453.2 (PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis melo])

HSP 1 Score: 335.5 bits (859), Expect = 2.9e-88
Identity = 162/178 (91.01%), Postives = 171/178 (96.07%), Query Frame = 0


Query: 1   MEGVVKKLERLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           MEGVVKKL  LAEKISEILGQSLGIKS+YFKERCEKGKSSFR+NRYPPCP AS+VYGLIP
Sbjct: 156 MEGVVKKLGSLAEKISEILGQSLGIKSNYFKERCEKGKSSFRMNRYPPCPIASQVYGLIP 215

Query: 61  HTDSDYLTILYQPQISGLQLMKAGKWFPVKPNSQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTD+DYLTILYQPQISGLQLMK+ KWFPVKPN QALL+NIGDLFQVVSND+FRSLKHRVV
Sbjct: 216 HTDTDYLTILYQPQISGLQLMKSAKWFPVKPNPQALLLNIGDLFQVVSNDIFRSLKHRVV 275

Query: 121 ASEEVERFSFAYFYCPSDDVMIESWLKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLSR 179
           AS  VER+SFAYFYCPSDDVMIESWLKPSIY+QFSYKEYRQQIEKDVEKTGNKVGLSR
Sbjct: 276 ASVGVERYSFAYFYCPSDDVMIESWLKPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSR 333

[Close]

BLAST of HG10011583 vs. NCBI nr
Match: XP_022985245.1 (gibberellin 2-beta-dioxygenase 8-like [Cucurbita maxima])

HSP 1 Score: 326.6 bits (836), Expect = 1.3e-85
Identity = 161/181 (88.95%), Postives = 167/181 (92.27%), Query Frame = 0


Query: 1   MEGVVKKLERLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           ME VVKK  RLAE I+EILGQSLGIKSSYFKERCEKG+SSFRLNRYPPCPFAS+VYGLIP
Sbjct: 160 MEAVVKKFGRLAESIAEILGQSLGIKSSYFKERCEKGRSSFRLNRYPPCPFASQVYGLIP 219

Query: 61  HTDSDYLTILYQPQISGLQLMKAGKWFPVKPNSQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTDSDYLTILYQ Q+SGLQLMK GKWF VKPN +ALLVNIGDLFQVVSND FRSLKHRVV
Sbjct: 220 HTDSDYLTILYQAQVSGLQLMKDGKWFSVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVV 279

Query: 121 ASEEVERFSFAYFYCPSDDVMIESWLKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180
           ASE VERFSFAYFY PSDDVMIES+ KPSIYRQFSYKEYRQQIEKDVEKTGNKVGL RF 
Sbjct: 280 ASEAVERFSFAYFYSPSDDVMIESYTKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLPRFL 339

Query: 181 L 182
           L
Sbjct: 340 L 340

[Close]

BLAST of HG10011583 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 207.6 bits (527), Expect = 1.2e-52
Identity = 97/179 (54.19%), Postives = 130/179 (72.63%), Query Frame = 0


Query: 1   MEGVVKKLERLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           ME    + E LA  ++E+L +  G  SS+FKE C +     R+NRYPPCP  S+VYGL+P
Sbjct: 155 MEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGLMP 214

Query: 61  HTDSDYLTILYQPQISGLQLMKAGKWFPVKPNSQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTDSD+LTILYQ Q+ GLQL+K  +W  VKPN +AL++NIGDLFQ  SN +++S++HRV+
Sbjct: 215 HTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVEHRVM 274

Query: 121 ASEEVERFSFAYFYCPSDDVMIESWLKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLSRF 180
            + +VERFS AYF CPS D +IE       YR FS++E+RQQ+++DV+K G KVGL RF
Sbjct: 275 TNPKVERFSTAYFMCPSYDAVIECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGLPRF 333

[Close]

BLAST of HG10011583 vs. ExPASy Swiss-Prot
Match: Q9C6I4 (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 6.3e-46
Identity = 87/181 (48.07%), Postives = 128/181 (70.72%), Query Frame = 0


Query: 1   MEGVVKKLERLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           +E  V+++ R+A+ I EILG+ + + S YF+   E   S  RLN+Y P  F S+V+GL+P
Sbjct: 156 VETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVP 215

Query: 61  HTDSDYLTILYQPQISGLQLMKAGKWFPVKPNSQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTD+ +LTIL Q QI GL+L   G+W  VKP  +AL VNIGD+FQ +SN V++S++HRV+
Sbjct: 216 HTDTSFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVI 275

Query: 121 ASEEVERFSFAYFYCPSDDVMIESWLKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180
           +   +ER S A+F CP  +  I+ +  P  YR+FS++EY++Q E DV++TG+KVGLSRF 
Sbjct: 276 SPANIERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRFL 335

Query: 181 L 182
           +
Sbjct: 336 I 336

[Close]

BLAST of HG10011583 vs. ExPASy Swiss-Prot
Match: Q7XP65 (Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX6 PE=1 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 3.8e-43
Identity = 82/187 (43.85%), Postives = 123/187 (65.78%), Query Frame = 0


Query: 1   MEGVVKKLERLAEKISEILGQSL-------GIKSSYFKERCEKGKSSFRLNRYPPCPFAS 60
           M+ V + + R+A  ++  L + L          + +F   C++     RLNRYP CPFA+
Sbjct: 166 MQEVAEAMSRVANTVAAALAEELTGRGGGGASAAPWFPAGCDETTCFLRLNRYPACPFAA 225

Query: 61  KVYGLIPHTDSDYLTILYQPQISGLQLMKAGKWFPVKPNSQALLVNIGDLFQVVSNDVFR 120
             +GL+PHTDSD+LT+L Q Q+ GL LMK  +W  V+P   AL+VNIGDLFQ  SN+ ++
Sbjct: 226 DTFGLVPHTDSDFLTVLCQDQVGGLHLMKDSRWVAVRPRPDALVVNIGDLFQAWSNNRYK 285

Query: 121 SLKHRVVASEEVERFSFAYFYCPSDDVMIESWLKPSIYRQFSYKEYRQQIEKDVEKTGNK 180
           S++H+VVA+ + +R S AYF CPS D ++ +  +PS YR F++ EYR+++++DV  TG K
Sbjct: 286 SVEHKVVANAKTDRLSVAYFLCPSYDSLVGTCGEPSPYRAFTFGEYRKKVQEDVRTTGKK 345

[Close]

BLAST of HG10011583 vs. ExPASy Swiss-Prot
Match: Q8LGZ9 (Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX5 PE=1 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 8.3e-38
Identity = 85/187 (45.45%), Postives = 117/187 (62.57%), Query Frame = 0


Query: 1   MEGVVKKLERLAEKISEILGQSL-GIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLI 60
           +E V + +  LA+K++EIL + L G           +     RLNRYPPC  A   +GL 
Sbjct: 152 IEEVSRAMYELAQKLAEILMRGLPGAGEGETMVTTREETCFLRLNRYPPCAMAMGGFGLC 211

Query: 61  PHTDSDYLTILYQPQ--ISGLQLMKAGKWFPVKPNSQALLVNIGDLFQVVSNDVFRSLKH 120
           PHTDSD LTI++Q Q  + GLQL+K G+W  VKP+   L+VN+GDL Q  SNDV++S++H
Sbjct: 212 PHTDSDLLTIVHQQQDTVGGLQLLKGGRWVAVKPSPSTLIVNVGDLLQAWSNDVYKSVEH 271

Query: 121 RVVASEEVERFSFAYFYCPSDDVMI-----ESWLKPSIYRQFSYKEYRQQIEKDVEKTGN 180
           RV+A+  +ERFS A+F CPS   +I           + YR F++ EYR+QI +DV  TG 
Sbjct: 272 RVMANATLERFSMAFFLCPSYHTLIIPSSSHVHDDDAHYRSFTFGEYRKQIMEDVRSTGR 331

[Close]

BLAST of HG10011583 vs. ExPASy Swiss-Prot
Match: Q39110 (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 124.4 bits (311), Expect = 1.3e-27
Identity = 60/135 (44.44%), Postives = 82/135 (60.74%), Query Frame = 0


Query: 8   LERLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIPHTDSDYL 67
           +  L+ KI E+LG SLG+K  YF+E  E+  S  RLN YPPC       G  PH D   L
Sbjct: 194 MSSLSLKIMELLGLSLGVKRDYFREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSL 253

Query: 68  TILYQPQISGLQLMKAGKWFPVKPNSQALLVNIGDLFQVVSNDVFRSLKHRVVASEEVER 127
           TIL+Q  ++GLQ+    +W  ++PN +A +VNIGD F  +SND ++S  HR V + E ER
Sbjct: 254 TILHQDHVNGLQVFVENQWRSIRPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESER 313

Query: 128 FSFAYFYCPSDDVMI 143
            S A+F CP  D ++
Sbjct: 314 KSLAFFLCPKKDRVV 328

[Close]

BLAST of HG10011583 vs. ExPASy TrEMBL
Match: A0A5D3C080 (Gibberellin 2-beta-dioxygenase 8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold68G00430 PE=4 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 5.7e-90
Identity = 164/181 (90.61%), Postives = 173/181 (95.58%), Query Frame = 0


Query: 1   MEGVVKKLERLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           MEGVVKKL  LAEKISEILGQSLGIKS+YFKERCEKGKSSFR+NRYPPCP AS+VYGLIP
Sbjct: 1   MEGVVKKLGSLAEKISEILGQSLGIKSNYFKERCEKGKSSFRMNRYPPCPIASQVYGLIP 60

Query: 61  HTDSDYLTILYQPQISGLQLMKAGKWFPVKPNSQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTD+DYLTILYQP ISGLQLMK+ KWFPVKPN QALL+NIGDLFQVVSND+FRSLKHRVV
Sbjct: 61  HTDTDYLTILYQPHISGLQLMKSAKWFPVKPNPQALLLNIGDLFQVVSNDIFRSLKHRVV 120

Query: 121 ASEEVERFSFAYFYCPSDDVMIESWLKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180
           AS  VER+SFAYFYCPSDDVMIESWLKPSIY+QFSYKEYRQQIEKDVEKTGNKVGLSRFF
Sbjct: 121 ASVGVERYSFAYFYCPSDDVMIESWLKPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180

Query: 181 L 182
           L
Sbjct: 181 L 181

[Close]

BLAST of HG10011583 vs. ExPASy TrEMBL
Match: A0A0A0L560 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G081890 PE=4 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 3.7e-89
Identity = 162/181 (89.50%), Postives = 173/181 (95.58%), Query Frame = 0


Query: 1   MEGVVKKLERLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           MEGVVKKL  LAEKISEILGQSLGIKS+YFKERCEKGKSSFR+NRYPPCP AS+VYGLIP
Sbjct: 1   MEGVVKKLGSLAEKISEILGQSLGIKSNYFKERCEKGKSSFRMNRYPPCPIASQVYGLIP 60

Query: 61  HTDSDYLTILYQPQISGLQLMKAGKWFPVKPNSQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTD+DYLTILYQPQISGLQL K+ KWFPVKPN +ALL+NIGDLFQVVSND+FRSLKHRVV
Sbjct: 61  HTDTDYLTILYQPQISGLQLKKSAKWFPVKPNPRALLLNIGDLFQVVSNDIFRSLKHRVV 120

Query: 121 ASEEVERFSFAYFYCPSDDVMIESWLKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180
           ASE VER+SFAYFYCPSDDV+IESWLKPSIY+ FSYKEYRQQIEKDVEKTGNKVGLSRFF
Sbjct: 121 ASEGVERYSFAYFYCPSDDVIIESWLKPSIYKHFSYKEYRQQIEKDVEKTGNKVGLSRFF 180

Query: 181 L 182
           L
Sbjct: 181 L 181

[Close]

BLAST of HG10011583 vs. ExPASy TrEMBL
Match: A0A1S3CJB6 (gibberellin 2-beta-dioxygenase 8-like OS=Cucumis melo OX=3656 GN=LOC103501620 PE=3 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 1.4e-88
Identity = 162/178 (91.01%), Postives = 171/178 (96.07%), Query Frame = 0


Query: 1   MEGVVKKLERLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           MEGVVKKL  LAEKISEILGQSLGIKS+YFKERCEKGKSSFR+NRYPPCP AS+VYGLIP
Sbjct: 156 MEGVVKKLGSLAEKISEILGQSLGIKSNYFKERCEKGKSSFRMNRYPPCPIASQVYGLIP 215

Query: 61  HTDSDYLTILYQPQISGLQLMKAGKWFPVKPNSQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTD+DYLTILYQPQISGLQLMK+ KWFPVKPN QALL+NIGDLFQVVSND+FRSLKHRVV
Sbjct: 216 HTDTDYLTILYQPQISGLQLMKSAKWFPVKPNPQALLLNIGDLFQVVSNDIFRSLKHRVV 275

Query: 121 ASEEVERFSFAYFYCPSDDVMIESWLKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLSR 179
           AS  VER+SFAYFYCPSDDVMIESWLKPSIY+QFSYKEYRQQIEKDVEKTGNKVGLSR
Sbjct: 276 ASVGVERYSFAYFYCPSDDVMIESWLKPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSR 333

[Close]

BLAST of HG10011583 vs. ExPASy TrEMBL
Match: A0A6J1JAT6 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC111483296 PE=3 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 6.5e-86
Identity = 161/181 (88.95%), Postives = 167/181 (92.27%), Query Frame = 0


Query: 1   MEGVVKKLERLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           ME VVKK  RLAE I+EILGQSLGIKSSYFKERCEKG+SSFRLNRYPPCPFAS+VYGLIP
Sbjct: 160 MEAVVKKFGRLAESIAEILGQSLGIKSSYFKERCEKGRSSFRLNRYPPCPFASQVYGLIP 219

Query: 61  HTDSDYLTILYQPQISGLQLMKAGKWFPVKPNSQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTDSDYLTILYQ Q+SGLQLMK GKWF VKPN +ALLVNIGDLFQVVSND FRSLKHRVV
Sbjct: 220 HTDSDYLTILYQAQVSGLQLMKDGKWFSVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVV 279

Query: 121 ASEEVERFSFAYFYCPSDDVMIESWLKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180
           ASE VERFSFAYFY PSDDVMIES+ KPSIYRQFSYKEYRQQIEKDVEKTGNKVGL RF 
Sbjct: 280 ASEAVERFSFAYFYSPSDDVMIESYTKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLPRFL 339

Query: 181 L 182
           L
Sbjct: 340 L 340

[Close]

BLAST of HG10011583 vs. ExPASy TrEMBL
Match: A0A6J1F0S8 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111438457 PE=3 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 7.2e-85
Identity = 159/181 (87.85%), Postives = 166/181 (91.71%), Query Frame = 0


Query: 1   MEGVVKKLERLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           ME VVKK  RLAE I+EILGQSLGIK+ YFKERCEKG+SSFRLNRYPPCPFAS+VYGLIP
Sbjct: 160 MEAVVKKFGRLAESIAEILGQSLGIKACYFKERCEKGRSSFRLNRYPPCPFASQVYGLIP 219

Query: 61  HTDSDYLTILYQPQISGLQLMKAGKWFPVKPNSQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTDSDYLTILYQ Q+SGLQLMK GKWF VKPN +ALLVNIGDLFQVVSND FRSLKHRVV
Sbjct: 220 HTDSDYLTILYQAQVSGLQLMKDGKWFAVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVV 279

Query: 121 ASEEVERFSFAYFYCPSDDVMIESWLKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180
           ASE VERFSFAYFY PSDDVMIES  KPSIYRQFSY+EYRQQIEKDVEKTGNKVGL RFF
Sbjct: 280 ASEAVERFSFAYFYSPSDDVMIESCTKPSIYRQFSYREYRQQIEKDVEKTGNKVGLPRFF 339

Query: 181 L 182
           L
Sbjct: 340 L 340

[Close]

BLAST of HG10011583 vs. TAIR 10
Match: AT4G21200.1 (gibberellin 2-oxidase 8 )

HSP 1 Score: 207.6 bits (527), Expect = 8.5e-54
Identity = 97/179 (54.19%), Postives = 130/179 (72.63%), Query Frame = 0


Query: 1   MEGVVKKLERLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           ME    + E LA  ++E+L +  G  SS+FKE C +     R+NRYPPCP  S+VYGL+P
Sbjct: 155 MEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGLMP 214

Query: 61  HTDSDYLTILYQPQISGLQLMKAGKWFPVKPNSQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTDSD+LTILYQ Q+ GLQL+K  +W  VKPN +AL++NIGDLFQ  SN +++S++HRV+
Sbjct: 215 HTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVEHRVM 274

Query: 121 ASEEVERFSFAYFYCPSDDVMIESWLKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLSRF 180
            + +VERFS AYF CPS D +IE       YR FS++E+RQQ+++DV+K G KVGL RF
Sbjct: 275 TNPKVERFSTAYFMCPSYDAVIECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGLPRF 333

[Close]

BLAST of HG10011583 vs. TAIR 10
Match: AT1G50960.1 (gibberellin 2-oxidase 7 )

HSP 1 Score: 185.3 bits (469), Expect = 4.5e-47
Identity = 87/181 (48.07%), Postives = 128/181 (70.72%), Query Frame = 0


Query: 1   MEGVVKKLERLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIP 60
           +E  V+++ R+A+ I EILG+ + + S YF+   E   S  RLN+Y P  F S+V+GL+P
Sbjct: 156 VETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVP 215

Query: 61  HTDSDYLTILYQPQISGLQLMKAGKWFPVKPNSQALLVNIGDLFQVVSNDVFRSLKHRVV 120
           HTD+ +LTIL Q QI GL+L   G+W  VKP  +AL VNIGD+FQ +SN V++S++HRV+
Sbjct: 216 HTDTSFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVI 275

Query: 121 ASEEVERFSFAYFYCPSDDVMIESWLKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLSRFF 180
           +   +ER S A+F CP  +  I+ +  P  YR+FS++EY++Q E DV++TG+KVGLSRF 
Sbjct: 276 SPANIERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRFL 335

Query: 181 L 182
           +
Sbjct: 336 I 336

[Close]

BLAST of HG10011583 vs. TAIR 10
Match: AT4G25420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 124.4 bits (311), Expect = 9.4e-29
Identity = 60/135 (44.44%), Postives = 82/135 (60.74%), Query Frame = 0


Query: 8   LERLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIPHTDSDYL 67
           +  L+ KI E+LG SLG+K  YF+E  E+  S  RLN YPPC       G  PH D   L
Sbjct: 194 MSSLSLKIMELLGLSLGVKRDYFREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSL 253

Query: 68  TILYQPQISGLQLMKAGKWFPVKPNSQALLVNIGDLFQVVSNDVFRSLKHRVVASEEVER 127
           TIL+Q  ++GLQ+    +W  ++PN +A +VNIGD F  +SND ++S  HR V + E ER
Sbjct: 254 TILHQDHVNGLQVFVENQWRSIRPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESER 313

Query: 128 FSFAYFYCPSDDVMI 143
            S A+F CP  D ++
Sbjct: 314 KSLAFFLCPKKDRVV 328

[Close]

BLAST of HG10011583 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 122.5 bits (306), Expect = 3.6e-28
Identity = 59/151 (39.07%), Postives = 84/151 (55.63%), Query Frame = 0


Query: 17  EILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIPHTDSDYLTILYQPQIS 76
           E + +SLG+          K      +N YP CP     YGL  H D++ +T+L Q ++S
Sbjct: 175 EAISESLGLAKDRVSNTIGKHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVS 234

Query: 77  GLQLMKAGKWFPVKPNSQALLVNIGDLFQVVSNDVFRSLKHRVVASEEVERFSFAYFYCP 136
           GLQ+ K GKW  V P     +VN+GD  QV+SN+ ++S+ HR V + ++ER S   FYCP
Sbjct: 235 GLQVFKDGKWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCP 294

Query: 137 SDDVMI--------ESWLKPSIYRQFSYKEY 160
           S+D +I        E    P+IYR F+Y EY
Sbjct: 295 SEDAVISPAQELINEEEDSPAIYRNFTYAEY 325

[Close]

BLAST of HG10011583 vs. TAIR 10
Match: AT5G51810.1 (gibberellin 20 oxidase 2 )

HSP 1 Score: 121.3 bits (303), Expect = 8.0e-28
Identity = 63/173 (36.42%), Postives = 92/173 (53.18%), Query Frame = 0


Query: 8   LERLAEKISEILGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIPHTDSDYL 67
           +  L+ KI E+LG SLG+   YF+   E+  S  RLN YPPC       G  PH D   L
Sbjct: 192 MSSLSLKIMELLGLSLGVNRDYFRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSL 251

Query: 68  TILYQPQISGLQLMKAGKWFPVKPNSQALLVNIGDLFQVVSNDVFRSLKHRVVASEEVER 127
           TIL+Q  ++GLQ+    +W  ++PN +A +VNIGD F  +SN +F+S  HR V + E  R
Sbjct: 252 TILHQDHVNGLQVFVDNQWQSIRPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESAR 311

Query: 128 FSFAYFYCPSDDVMIE-----------------SWLKPSIYRQFSYKEYRQQI 164
            S A+F CP  D +++                 +W   S++ +F+ K YR  +
Sbjct: 312 KSMAFFLCPKKDKVVKPPSDILEKMKTRKYPDFTW---SMFLEFTQKHYRADV 361

The following BLAST results are available for this feature:

BLAST of HG10011583 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038906005.1	2.7e-94	96.13	gibberellin 2-beta-dioxygenase 8-like [Benincasa hispida]	[more]
TYK04745.1	1.2e-89	90.61	gibberellin 2-beta-dioxygenase 8-like [Cucumis melo var. makuwa]	[more]
XP_004152933.2	7.6e-89	89.50	gibberellin 2-beta-dioxygenase 8 [Cucumis sativus] >XP_031737838.1 gibberellin 2...	[more]
XP_008463453.2	2.9e-88	91.01	PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis melo]	[more]
XP_022985245.1	1.3e-85	88.95	gibberellin 2-beta-dioxygenase 8-like [Cucurbita maxima]	[more]

BLAST of HG10011583 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49561	1.2e-52	54.19	Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ...	[more]
Q9C6I4	6.3e-46	48.07	Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 ...	[more]
Q7XP65	3.8e-43	43.85	Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
Q8LGZ9	8.3e-38	45.45	Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
Q39110	1.3e-27	44.44	Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2	[more]

BLAST of HG10011583 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3C080	5.7e-90	90.61	Gibberellin 2-beta-dioxygenase 8-like OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A0A0L560	3.7e-89	89.50	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]
A0A1S3CJB6	1.4e-88	91.01	gibberellin 2-beta-dioxygenase 8-like OS=Cucumis melo OX=3656 GN=LOC103501620 PE...	[more]
A0A6J1JAT6	6.5e-86	88.95	gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC11148329...	[more]
A0A6J1F0S8	7.2e-85	87.85	gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111438...	[more]

BLAST of HG10011583 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G21200.1	8.5e-54	54.19	gibberellin 2-oxidase 8	[more]
AT1G50960.1	4.5e-47	48.07	gibberellin 2-oxidase 7	[more]
AT4G25420.1	9.4e-29	44.44	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G10490.1	3.6e-28	39.07	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G51810.1	8.0e-28	36.42	gibberellin 20 oxidase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..178 e-value: 4.3E-52 score: 179.4
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 41..136 e-value: 2.9E-22 score: 79.0
None	No IPR available	PANTHER	PTHR47990:SF164	GIBBERELLIN 2-BETA-DIOXYGENASE 8-LIKE	coord: 5..175
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 5..175
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 2..163
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 37..136 score: 11.48253

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10011583.1	HG10011583.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding