HG10010998 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10010998
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	dihydrofolate synthetase isoform X1
Location	Chr01: 1433278 .. 1438197 (+)
RNA-Seq Expression	HG10010998
Synteny	HG10010998

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATGTCTTCAAATATCATCACCGCTGTCGCTCACAGATTCTCAGAAGCCTTCTCCTAAATCATTTCGTTCGTGAATGGCCTTCTATCAGTACCCGAATAGGGTCCAAACAATGCTTCGGCACTCACTCAGAAGATCAACAGATAACCGAATTCATGGAATATTTAGATTCCCTGAAGAACTACGAGAAATTGGGTGTGCCCAGAGGCGCAGGCACTGATTCTGATGATGGGTTCGATCTCGGAAGGATGAGAAGGCTGATGGAGCGCCTGGGTAATCCACAATCGAAGTTTAAGGTTGCCTCTTAGTTTTCTTAGCCTTAACTAAATCTTGCAGTTTCGAACTCACCCATATCCTCTGCTATCAACTGAGTTAAATTAGACTACTAAAAATATTTATTCGATCACTGCTAAGCCTAAAAGCTTGTAGATTTAAGCTTTTATTAATATTAATACTCTCCTCACTTGTGATGGTTATCAATACCAATCGATGGGAAATGAGATTACCAATTATTGAAAATTCCACATGAAAAGTTGAAGCTCATAGGAGCGTTTGGGACACTGAATTAGTTATTATAGTTTGTTGGTTATTATAGTATGTGGTTATAATATCTATAGGTTATAAACTTATAATAGTTATACCTTAGCAAAGACGAATGGAGCGAAAGAGAGGATAAGCAGGAAATAGTAAATACCGTAGCAAAGAAGGATTTGAAATAGTAATATTGTAGTTAATTGTAAGTTATAATAATGGTAAAAACCAACTATTATAATTGGTGGCCCAAACACCCCGTAGTGACTGATGGTAGGACAATTGTAGGCAATTCACATTGCGGGAACGAAAGGAAAAGGGTCAACTGCAGCCTTCCTTTCTAACATTTTACGAGCAGAGGGATACTCTGTTGGTTGTTATACTAGGTACGGCCTTTGTGCAACAATTATCAATTTTTCTGTATACTCTTACTTGGGAGTGATTTGAGAAGGCATAACTTTGTGCTTGTGAATTTCTTCATGCGTAGTCCTCATATTGAAACTATAAGGGAACGTATTTCACTCGGACAAACTGGAGAGATGGTCTCTGGGAAGGCACTAAACTCTCTTTTCAAAAGGAATGAAGAGTTCTTTGATCAGTCAGTAGAGCTTGAAAATGGACGTATAAGTTACTTCGAGGTATTAATTCATGCTTTTTGTTGTTGTGATTGAAAATTCTCTAGGAACCCAAATGGCTTATTTGCTGCTTCTGATTCTTCTACATGATTTGATTAAGCAAATTGATGTTCCATGTCTAGGTTTTCACTTTGTAAATTTGTATGTCTTGTGATCTTTTATGGTATATCCTGCATCTTTTTGGGCTTGGTTTCATCTAGGAAGATATAAATTGTTCATGTCATTGATAAACCTTTGAACCCTTAAAGTTCATTGGACCTATGCAGTCATTAATGTTTTCTTCAATCTTTTAGACGAGCTGATGAAATTCTTCCAGGTCTTTACTGCTATGGCATTCTCGCTCTTTGCTCAGGAGAATGTTGATGTTGCAGTCATTGAGGTTCGCTTTCTTTTAGACTAATTTGTTCTTTGTCAAGTATTTTTTTATTTCTCCAGCAAAAGCTTATAAGGCCATTGTCAATATGTTCTTATTTTAACTGAAACAGGCTGGACTTGGTGGTGCAAGAGATGCAACAAACATAATTTGCAGCTCTAGACTTGCTGCTGCAGTCATAACTTCAATTGGAGAGGAACACATGGCTGCCCTGGGTGGCTCTTTGGAAAGCATTGCAAGGGCAAAGGCTGGAATAATTAAACGTGGTTGTCCAGTTAGGTTGTTTTCTTGATGTAATTTTGATGCTGTTTGACTTTGTTCAGCCTTCGATATTCATTATCCACGCATTAGTTAGTTTTTCCTTTTCTAATGAGTTGACATGACCTACAAGAAGTGAAGAGGGGACCTAAAGTCAGCCTGAACTTACATAAGACAATGAAGATCATGAAAATGCCCTCTAATTAGCCATAATTATTGTTTGAAATAATATTGGTGGTTGAAAAACTAAACTGAAGTTTTTGCAGTCTGTCTTTCAAATTATATTGCCATATAGGAAGGTATTAAATTAGTCTATTGCTGTGAAGTGTAAACAACGTAGAAGCAAGCTGCATGAAAAGGTCTTAGAGGCTTCCAAAGTTTATTGCCTTTATTTGGCCTCTACCGGCATGGAATTTTCTCTCCCAAAGTTGTTGATGCAAGTCTATGTGTTTGTTTGTCTGTGTCTGTGTGTGAAAGAGAAGCTTATTCTCCAACTCCTATTGTGCTAATACATTCCTGTTTCATTCTATTTTTTTTTCTCTCTTCTGAAATTTATTCCTAAAAGGCTCGTTAATTGATTTCAGACCATTCTGGGTGGTCCATTCCTTCCAAATATTGAGTGCATTCTTCGCGATAAAGCATTGTTCATGTCTTCGCCTGTAATATCAGCCTCAGGTCCGGGAAATAGAAGTACCATAAAAGGTGTAAGCCTGCTCAATGGAGGACTTTGCCAATGCTGCGACATAGTAATTCAAATTGACAATGAAGTATGGCTCGTGTATTCTTAAACAAATATAAATTTCACTCCTTGAATTTCAAGCAAGAGTTCCTTAATCTCATTGTGAATGCAGTTTATCGAGTTATTGGATGTCAACCTCCGCATGCTTGGACGTCATCAACTTCAGAATGCAGCAACTGCAACTTGTGTTATTCTTACTCTCCGCAATTTAGGTATAATATCACTTTATATATTTGATTTGACATCCGTACCTTCATTTAGAGAATAAACATGTGATGTGTACTGATTTCCCTGACACGTTTACTGAAGGTAATAGCTTGTGTGTGTTGCATCTACACTTTCTTTTTGAAGTTTCTGAACAGTTTGTGTCCTTTGCATGTGAGGCCTGTGCAGATAGGATACTTTAGAGACAGTTTTGCTAAAGTCTTAGGGAAAGATACTTGTAATTTCTTGCTTTTAAGTTTGAGATGATATATGATGCAAGATAGATTGTAGTTTATTTTACTGAATACTTTTAATATTTTCCTATTTTGATAAATAACTCAGGTTGGAGAATTTCGGATGCATCTATCAGGAGTGGACTAGAGCAAACATTCCTGGTCGGTAGGAGTCATTTCTTACCAGCCAAGGAAGCTGGGGTTCTTGGACTACCTGGAGCAACAATATTGCTGGACGGAGGTGCATTCATATTTCTTTTTACTAATGTCAAGTTTGGTCTGTTGGGACCTGAGAATTGTGCTTGTGCTGCGTCTTCTCTACCGTATTGTACTGAAAGACTTTTGGTAGATGTTCAGTACATAGGCACTCTTTTGGCTGTTATTGGTAAATGTTTATATCTGGTCTGCTGACTGCTTAAAAGTTTGCAGAATATTGTAGAACTACTTGTGTTAAAATTTCAACCTGCATTAACTGATATATATTGGAGTTGTGTTGTTCTGGATGTTGGTGCCATGTCTTGCATTCTTTTAGACAGCCTTTCTAATTGGTTCTTGAATGTTTTCTCAGCCCACACCAAAGACTCTGCTAAAGCATTAGTGGATACCATTCAAATGGCTTTTCCTGAGGATCGATTGGCTCTTGTAGTTGCGATGGCTAATGATAAGGATCACGTTGGTTTTGCCAGAGAATTCCTTCGAGGTATAGCTTTCCCCTGCAAAGAGTTGGTCTCATATTTTCTTCACCACAGAAGTTTCTTCTTGATTTTAAATTCTTCGATACTCTTGAATCTTTTGGATTATATAGGCCTCATCTGGTTCTGATAACCATGTTCCTTTTGAATCATATGAGAATGTCTGCAGAGTTAGATTAGCTAAAGTGTGCATAGATCGAAATAGATGACTGGAAGAAAGTGTGTTAAGAGATGTTGGTAAGAGAAAATATGTTTAAAGAGTGAATAAACTAAAATTGTTAGCGACAGTTTTATCTAGTTAACTTATGATAGGTTCTCTTAAATATTAGTTTAAGTTTCAAAATTTAGTTAAAAGAAGCTTAACAAATGAAAACGGAAACAAAACTATCTTCTTTATTTATAAATAGGAGGCCTAAAATGAGGTATGGACTCCTTCATCAAATTACATTCTTCCTATTCTACCATTTCTCTTCATTGTTTTTTAGTTAAACGAAAAAATGAGACCTTTAATTGAAGTAATGAAAAGAAACAAATGAACTCCCCAAGGGAATCAAAAAAGCAAAGAGAAAAAATATAAATAAAGTGAGCCTAGTAAGCACTCCAATTAATACAACTTACAAATATCCTGGGAAGCAAAATCAACTAACAATTCAGGAAAAGAATATCATTGATAAACTTTTAATATGGAAATATCAAAGCGCTCTAGGCAACACTGCTGCTTATTCTCAAAAGTCTTTTGCTTATGCTCAAATCAAATCTCAGAAAGGATTGCAATTTAAACAAAACAATAACTTTTTGTAGAGATTCTGTACAACTGTACATATGTTACGCAATGTCATGTAATCTTGTTGCTCGTCTCTGTCTTCTTTGATGTTGTAAGATGTAGCAATGAAATGCTGACAGTCAGCAGTTAACTGAATTTCAGGTGGAAAATTGGAGTCCATTGTCTTAACTGAAGCCCATATTTATGGAGGCAAATCAAGGACAACTTCAGCTGCTTTTCTAAGAGACTGCTGGATCCAAGCATCCAATGAATTGGGGATCCCTATTTCTTTAGAAACTAAAGATGCACTAGTTTCCTTCACAAGCAAACTCGAAAACAGACCGTTATTGACTACCGAGCCCTCGTTATTACATGCCATAAAAATCGCTGCTGAGATTCTCAAGCAGAGGACGGAAGGGCGGCGAGGTCTTGTCATAGTAACTGGCTCTCTGCATGCTGTTTCAATGGTGTTATCTTCTCTTCATTCTTGA

mRNA sequence

ATGAATGTCTTCAAATATCATCACCGCTGTCGCTCACAGATTCTCAGAAGCCTTCTCCTAAATCATTTCGTTCGTGAATGGCCTTCTATCAGTACCCGAATAGGGTCCAAACAATGCTTCGGCACTCACTCAGAAGATCAACAGATAACCGAATTCATGGAATATTTAGATTCCCTGAAGAACTACGAGAAATTGGGTGTGCCCAGAGGCGCAGGCACTGATTCTGATGATGGGTTCGATCTCGGAAGGATGAGAAGGCTGATGGAGCGCCTGGGTAATCCACAATCGAAGTTTAAGGCAATTCACATTGCGGGAACGAAAGGAAAAGGGTCAACTGCAGCCTTCCTTTCTAACATTTTACGAGCAGAGGGATACTCTGTTGGTTGTTATACTAGTCCTCATATTGAAACTATAAGGGAACGTATTTCACTCGGACAAACTGGAGAGATGGTCTCTGGGAAGGCACTAAACTCTCTTTTCAAAAGGAATGAAGAGTTCTTTGATCAGTCAGTAGAGCTTGAAAATGGACGTATAAGTTACTTCGAGGTCTTTACTGCTATGGCATTCTCGCTCTTTGCTCAGGAGAATGTTGATGTTGCAGTCATTGAGGCTGGACTTGGTGGTGCAAGAGATGCAACAAACATAATTTGCAGCTCTAGACTTGCTGCTGCAGTCATAACTTCAATTGGAGAGGAACACATGGCTGCCCTGGGTGGCTCTTTGGAAAGCATTGCAAGGGCAAAGGCTGGAATAATTAAACGTGGTTGTCCAACCATTCTGGGTGGTCCATTCCTTCCAAATATTGAGTGCATTCTTCGCGATAAAGCATTGTTCATGTCTTCGCCTGTAATATCAGCCTCAGGTCCGGGAAATAGAAGTACCATAAAAGGTGTAAGCCTGCTCAATGGAGGACTTTGCCAATGCTGCGACATAGTAATTCAAATTGACAATGAATTTATCGAGTTATTGGATGTCAACCTCCGCATGCTTGGACGTCATCAACTTCAGAATGCAGCAACTGCAACTTGTGTTATTCTTACTCTCCGCAATTTAGGTTGGAGAATTTCGGATGCATCTATCAGGAGTGGACTAGAGCAAACATTCCTGGTCGGTAGGAGTCATTTCTTACCAGCCAAGGAAGCTGGGGTTCTTGGACTACCTGGAGCAACAATATTGCTGGACGGAGGTGCATTCATATTTCTTTTTACTAATGTCAAGTTTGGTCTGTTGGGACCTGAGAATTGTGCTTGTGCTGCGTCTTCTCTACCGTATTGTACTGAAAGACTTTTGGTAGATGTTCAGTACATAGGCACTCTTTTGGCTGTTATTGCCCACACCAAAGACTCTGCTAAAGCATTAGTGGATACCATTCAAATGGCTTTTCCTGAGGATCGATTGGCTCTTGTAGTTGCGATGGCTAATGATAAGGATCACGTTGGTTTTGCCAGAGAATTCCTTCGAGGTGGAAAATTGGAGTCCATTGTCTTAACTGAAGCCCATATTTATGGAGGCAAATCAAGGACAACTTCAGCTGCTTTTCTAAGAGACTGCTGGATCCAAGCATCCAATGAATTGGGGATCCCTATTTCTTTAGAAACTAAAGATGCACTAGTTTCCTTCACAAGCAAACTCGAAAACAGACCGTTATTGACTACCGAGCCCTCGTTATTACATGCCATAAAAATCGCTGCTGAGATTCTCAAGCAGAGGACGGAAGGGCGGCGAGGTCTTGTCATAGTAACTGGCTCTCTGCATGCTGTTTCAATGGTGTTATCTTCTCTTCATTCTTGA

Coding sequence (CDS)

ATGAATGTCTTCAAATATCATCACCGCTGTCGCTCACAGATTCTCAGAAGCCTTCTCCTAAATCATTTCGTTCGTGAATGGCCTTCTATCAGTACCCGAATAGGGTCCAAACAATGCTTCGGCACTCACTCAGAAGATCAACAGATAACCGAATTCATGGAATATTTAGATTCCCTGAAGAACTACGAGAAATTGGGTGTGCCCAGAGGCGCAGGCACTGATTCTGATGATGGGTTCGATCTCGGAAGGATGAGAAGGCTGATGGAGCGCCTGGGTAATCCACAATCGAAGTTTAAGGCAATTCACATTGCGGGAACGAAAGGAAAAGGGTCAACTGCAGCCTTCCTTTCTAACATTTTACGAGCAGAGGGATACTCTGTTGGTTGTTATACTAGTCCTCATATTGAAACTATAAGGGAACGTATTTCACTCGGACAAACTGGAGAGATGGTCTCTGGGAAGGCACTAAACTCTCTTTTCAAAAGGAATGAAGAGTTCTTTGATCAGTCAGTAGAGCTTGAAAATGGACGTATAAGTTACTTCGAGGTCTTTACTGCTATGGCATTCTCGCTCTTTGCTCAGGAGAATGTTGATGTTGCAGTCATTGAGGCTGGACTTGGTGGTGCAAGAGATGCAACAAACATAATTTGCAGCTCTAGACTTGCTGCTGCAGTCATAACTTCAATTGGAGAGGAACACATGGCTGCCCTGGGTGGCTCTTTGGAAAGCATTGCAAGGGCAAAGGCTGGAATAATTAAACGTGGTTGTCCAACCATTCTGGGTGGTCCATTCCTTCCAAATATTGAGTGCATTCTTCGCGATAAAGCATTGTTCATGTCTTCGCCTGTAATATCAGCCTCAGGTCCGGGAAATAGAAGTACCATAAAAGGTGTAAGCCTGCTCAATGGAGGACTTTGCCAATGCTGCGACATAGTAATTCAAATTGACAATGAATTTATCGAGTTATTGGATGTCAACCTCCGCATGCTTGGACGTCATCAACTTCAGAATGCAGCAACTGCAACTTGTGTTATTCTTACTCTCCGCAATTTAGGTTGGAGAATTTCGGATGCATCTATCAGGAGTGGACTAGAGCAAACATTCCTGGTCGGTAGGAGTCATTTCTTACCAGCCAAGGAAGCTGGGGTTCTTGGACTACCTGGAGCAACAATATTGCTGGACGGAGGTGCATTCATATTTCTTTTTACTAATGTCAAGTTTGGTCTGTTGGGACCTGAGAATTGTGCTTGTGCTGCGTCTTCTCTACCGTATTGTACTGAAAGACTTTTGGTAGATGTTCAGTACATAGGCACTCTTTTGGCTGTTATTGCCCACACCAAAGACTCTGCTAAAGCATTAGTGGATACCATTCAAATGGCTTTTCCTGAGGATCGATTGGCTCTTGTAGTTGCGATGGCTAATGATAAGGATCACGTTGGTTTTGCCAGAGAATTCCTTCGAGGTGGAAAATTGGAGTCCATTGTCTTAACTGAAGCCCATATTTATGGAGGCAAATCAAGGACAACTTCAGCTGCTTTTCTAAGAGACTGCTGGATCCAAGCATCCAATGAATTGGGGATCCCTATTTCTTTAGAAACTAAAGATGCACTAGTTTCCTTCACAAGCAAACTCGAAAACAGACCGTTATTGACTACCGAGCCCTCGTTATTACATGCCATAAAAATCGCTGCTGAGATTCTCAAGCAGAGGACGGAAGGGCGGCGAGGTCTTGTCATAGTAACTGGCTCTCTGCATGCTGTTTCAATGGTGTTATCTTCTCTTCATTCTTGA

Protein sequence

MNVFKYHHRCRSQILRSLLLNHFVREWPSISTRIGSKQCFGTHSEDQQITEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGQTGEMVSGKALNSLFKRNEEFFDQSVELENGRISYFEVFTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGSLESIARAKAGIIKRGCPTILGGPFLPNIECILRDKALFMSSPVISASGPGNRSTIKGVSLLNGGLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASIRSGLEQTFLVGRSHFLPAKEAGVLGLPGATILLDGGAFIFLFTNVKFGLLGPENCACAASSLPYCTERLLVDVQYIGTLLAVIAHTKDSAKALVDTIQMAFPEDRLALVVAMANDKDHVGFAREFLRGGKLESIVLTEAHIYGGKSRTTSAAFLRDCWIQASNELGIPISLETKDALVSFTSKLENRPLLTTEPSLLHAIKIAAEILKQRTEGRRGLVIVTGSLHAVSMVLSSLHS

Homology

[Close]

BLAST of HG10010998 vs. NCBI nr
Match: XP_038905573.1 (dihydrofolate synthetase [Benincasa hispida])

HSP 1 Score: 958.7 bits (2477), Expect = 2.3e-275
Identity = 500/596 (83.89%), Postives = 521/596 (87.42%), Query Frame = 0


Query: 1   MNVFKYHHRCRSQILRSLLLNHFVREWPSISTRIGSKQCFGTHSEDQQITEFMEYLDSLK 60
           M +FKYHH CR QIL +LLLNHF  E PSIS RIGSKQCF TH EDQ +TEFMEYLDSLK
Sbjct: 1   MKIFKYHHHCRPQILGTLLLNHFRGECPSISVRIGSKQCFSTHPEDQHMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP+GAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPKGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGQTGEMVSGKALNSLFKRNEEFFDQSVELENGRISY 180
           RAEGYS GCYTSPHIETIRERISLG++GEMVSGKALNSLFKRN+EFFDQSVELENGRIS+
Sbjct: 121 RAEGYSFGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFFDQSVELENGRISH 180

Query: 181 FEVFTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEV TAMAF LFAQENVD+AVIEAGLGGARDATNIICSS LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFLLFAQENVDLAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIARAKAGIIKRGCPTILGGPFLPNIECILRDKALFMSSPVISASGPGNRSTIKGVSL 300
           LESIA AKAGIIKRGCP ILGGPFLP+IE ILRDKAL MSSPVISASGPGNRSTIKGVSL
Sbjct: 241 LESIATAKAGIIKRGCPAILGGPFLPSIERILRDKALSMSSPVISASGPGNRSTIKGVSL 300

Query: 301 LNGGLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360
           LNGG+CQCCD+VIQID+EFIELLDVNLRMLG HQLQNAATATCVIL LRNLGWRISDAS+
Sbjct: 301 LNGGICQCCDVVIQIDDEFIELLDVNLRMLGHHQLQNAATATCVILALRNLGWRISDASV 360

Query: 361 RSGLEQTFLVGRSHFLPAKEAGVLGLPGATILLDGGAFIFLFTNVKFGLLGPENCACAAS 420
           RSGLEQTFLVGRSHFLPAKEAGVLGLPGATILLDG                         
Sbjct: 361 RSGLEQTFLVGRSHFLPAKEAGVLGLPGATILLDG------------------------- 420

Query: 421 SLPYCTERLLVDVQYIGTLLAVIAHTKDSAKALVDTIQMAFPEDRLALVVAMANDKDHVG 480
                                  AHTKDSAKALVDTIQMAFPE RLALVVAMA+DKDH+G
Sbjct: 421 -----------------------AHTKDSAKALVDTIQMAFPEARLALVVAMASDKDHIG 480

Query: 481 FAREFLRGGKLESIVLTEAHIYGGKSRTTSAAFLRDCWIQASNELGIPISLETKDALVSF 540
           FAREFL+GGKLESIVLTEAHI GGKSRTTSA FLRDCWIQASNELGIPISLETKDA VSF
Sbjct: 481 FAREFLQGGKLESIVLTEAHIGGGKSRTTSAVFLRDCWIQASNELGIPISLETKDAPVSF 540

Query: 541 TSKLENRPLLTTEPSLLHAIKIAAEILKQRTEGRRGLVIVTGSLHAVSMVLSSLHS 597
           TSKLENRP+LTTE SLLHAIK+AAEILKQRTEGR+GLV+VTGSLHAVSMVLS LHS
Sbjct: 541 TSKLENRPVLTTETSLLHAIKVAAEILKQRTEGRQGLVVVTGSLHAVSMVLSFLHS 548

[Close]

BLAST of HG10010998 vs. NCBI nr
Match: XP_008465388.1 (PREDICTED: dihydrofolate synthetase isoform X1 [Cucumis melo])

HSP 1 Score: 942.2 bits (2434), Expect = 2.2e-270
Identity = 494/596 (82.89%), Postives = 519/596 (87.08%), Query Frame = 0


Query: 1   MNVFKYHHRCRSQILRSLLLNHFVREWPSISTRIGSKQCFGTHSEDQQITEFMEYLDSLK 60
           MNVFKYHH CR QIL SLLLNHF+ E PSIS+R GSKQCF THSEDQ +TEFMEYLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGS AAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGQTGEMVSGKALNSLFKRNEEFFDQSVELENGRISY 180
           RAEGYSVGCYTSPHIET+RERISLG++GEMVSGKALNSLFKRN+E FDQSVELE+GRIS+
Sbjct: 121 RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH 180

Query: 181 FEVFTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEV TAMAFSLFAQENVDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIARAKAGIIKRGCPTILGGPFLPNIECILRDKALFMSSPVISASGPGNRSTIKGVSL 300
           LESIA AKAGIIKRGCPTILGG FLP IE ILRDKAL MSSPVI+ S PGNRSTIKGV++
Sbjct: 241 LESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSDPGNRSTIKGVNM 300

Query: 301 LNGGLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360
           LNGGLCQCCDIVIQIDNEF+ELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI
Sbjct: 301 LNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 361 RSGLEQTFLVGRSHFLPAKEAGVLGLPGATILLDGGAFIFLFTNVKFGLLGPENCACAAS 420
           RSGLEQTFL+GRSHFL AKEA VLGLPGATILLDG                         
Sbjct: 361 RSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDG------------------------- 420

Query: 421 SLPYCTERLLVDVQYIGTLLAVIAHTKDSAKALVDTIQMAFPEDRLALVVAMANDKDHVG 480
                                  AHTKDSAKALVDTIQMAFPE RLALV+AMA+DK+HV 
Sbjct: 421 -----------------------AHTKDSAKALVDTIQMAFPEARLALVIAMASDKNHVD 480

Query: 481 FAREFLRGGKLESIVLTEAHIYGGKSRTTSAAFLRDCWIQASNELGIPISLETKDALVSF 540
           FAREFL+GGKLE +VLTEAHI GGKSRTTSAAFLRDCWIQAS ELGIPISLETKDA VSF
Sbjct: 481 FAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRDCWIQASIELGIPISLETKDAEVSF 540

Query: 541 TSKLENRPLLTTEPSLLHAIKIAAEILKQRTEGRRGLVIVTGSLHAVSMVLSSLHS 597
           TSKLENRP+LTTE SLLHAIKIAAEILKQRT+G++ LV+V+GSLHAVSMVLSSLHS
Sbjct: 541 TSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQSLVVVSGSLHAVSMVLSSLHS 548

[Close]

BLAST of HG10010998 vs. NCBI nr
Match: XP_022141068.1 (dihydrofolate synthetase isoform X1 [Momordica charantia])

HSP 1 Score: 936.8 bits (2420), Expect = 9.2e-269
Identity = 495/596 (83.05%), Postives = 516/596 (86.58%), Query Frame = 0


Query: 1   MNVFKYHHRCRSQILRSLLLNHFVREWPSISTRIGSKQCFGTHSEDQQITEFMEYLDSLK 60
           MNVFKYH R R QIL  LLLN  V E PSIS RIGSKQCF THSED ++TEFMEYLDSLK
Sbjct: 1   MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGQTGEMVSGKALNSLFKRNEEFFDQSVELENGRISY 180
           RAEGYSVGCYTSPHIETIRERISLG++GEMVSGKALNSLFKRN+EF DQSVE ENGRIS+
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180

Query: 181 FEVFTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEV TAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIARAKAGIIKRGCPTILGGPFLPNIECILRDKALFMSSPVISASGPGNRSTIKGVSL 300
           LESIA AKAGIIK GCPTILGGPFLP+IECIL DKAL MSS VISAS PG+RSTIKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSL 300

Query: 301 LNGGLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360
           L+G LCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATC ILTLRNLGWRISD+SI
Sbjct: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360

Query: 361 RSGLEQTFLVGRSHFLPAKEAGVLGLPGATILLDGGAFIFLFTNVKFGLLGPENCACAAS 420
           ++GLEQTFLVGRSHFL AKEAGVLGLPGATILLDG                         
Sbjct: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDG------------------------- 420

Query: 421 SLPYCTERLLVDVQYIGTLLAVIAHTKDSAKALVDTIQMAFPEDRLALVVAMANDKDHVG 480
                                  AHTKDSAKALVDTIQM FPE RLALVVAMA+DKDHVG
Sbjct: 421 -----------------------AHTKDSAKALVDTIQMTFPEARLALVVAMASDKDHVG 480

Query: 481 FAREFLRGGKLESIVLTEAHIYGGKSRTTSAAFLRDCWIQASNELGIPISLETKDALVSF 540
           FAREFL+GGKLESI+LTEA+I GGKSRTTSAA LRDCWIQA+NELGIPISLETKDALVS 
Sbjct: 481 FAREFLQGGKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIPISLETKDALVSS 540

Query: 541 TSKLENRPLLTTEPSLLHAIKIAAEILKQRTEGRRGLVIVTGSLHAVSMVLSSLHS 597
           TSKLE+RP+LTTE SLLHA+KIAAEILKQRTEGR GL +VTGSLHAVSMVLSSLHS
Sbjct: 541 TSKLESRPILTTETSLLHAMKIAAEILKQRTEGRAGLTVVTGSLHAVSMVLSSLHS 548

[Close]

BLAST of HG10010998 vs. NCBI nr
Match: XP_004149457.1 (dihydrofolate synthetase isoform X2 [Cucumis sativus])

HSP 1 Score: 935.3 bits (2416), Expect = 2.7e-268
Identity = 490/596 (82.21%), Postives = 519/596 (87.08%), Query Frame = 0


Query: 1   MNVFKYHHRCRSQILRSLLLNHFVREWPSISTRIGSKQCFGTHSEDQQITEFMEYLDSLK 60
           MNVFKYHH CR QIL SLLLNHF+ E PSIS+R GSKQCF THSEDQ +T+FMEYLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRG+GTDSDDGFDLGRMRRLMERLGNPQS+FKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGQTGEMVSGKALNSLFKRNEEFFDQSVELENGRISY 180
           R EGYSVGCYTSPHIETIRERISLG++G+MVSGKALNSLFKRN+E FDQSVELENG +S+
Sbjct: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180

Query: 181 FEVFTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEV TAMAFSLFAQE+VDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEH+AALGGS
Sbjct: 181 FEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGS 240

Query: 241 LESIARAKAGIIKRGCPTILGGPFLPNIECILRDKALFMSSPVISASGPGNRSTIKGVSL 300
           LESIA AKAGIIKRGCPTILGGPFLP IE ILRDKAL MSSPVISAS PGNRSTIKGV+L
Sbjct: 241 LESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNL 300

Query: 301 LNGGLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360
           LNGGL QCCDIVIQIDNEFIELLDVNLRMLG HQLQNAATATCVILTLRNLGWRISDASI
Sbjct: 301 LNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 361 RSGLEQTFLVGRSHFLPAKEAGVLGLPGATILLDGGAFIFLFTNVKFGLLGPENCACAAS 420
           RSGLEQTFL+GRSHFL A+EA VLGLPGATILLDG                         
Sbjct: 361 RSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDG------------------------- 420

Query: 421 SLPYCTERLLVDVQYIGTLLAVIAHTKDSAKALVDTIQMAFPEDRLALVVAMANDKDHVG 480
                                  AHTKDSAKAL+DTIQMAFPE +LALVVAMA+DK+HVG
Sbjct: 421 -----------------------AHTKDSAKALLDTIQMAFPEAQLALVVAMASDKNHVG 480

Query: 481 FAREFLRGGKLESIVLTEAHIYGGKSRTTSAAFLRDCWIQASNELGIPISLETKDALVSF 540
           FAREFL+GGKLES+VLTEA I GGKSRTTSAAFLRDCWIQASNELGIPISLETKDA V F
Sbjct: 481 FAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRDCWIQASNELGIPISLETKDAEVFF 540

Query: 541 TSKLENRPLLTTEPSLLHAIKIAAEILKQRTEGRRGLVIVTGSLHAVSMVLSSLHS 597
           TSKL NRP+LTTE SLLHAIKIAAEILKQRT+GR+GLV+V+GSLHAVSMVL+SLHS
Sbjct: 541 TSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGRQGLVVVSGSLHAVSMVLASLHS 548

[Close]

BLAST of HG10010998 vs. NCBI nr
Match: XP_023553540.1 (dihydrofolate synthetase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 927.9 bits (2397), Expect = 4.3e-266
Identity = 487/596 (81.71%), Postives = 515/596 (86.41%), Query Frame = 0


Query: 1   MNVFKYHHRCRSQILRSLLLNHFVREWPSISTRIGSKQCFGTHSEDQQITEFMEYLDSLK 60
           MN+FKYHH  R QI   LLLN+FV E PSIS+RIGSKQCFG HSEDQQ+TEFMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQQMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGTDS+DGFDLGRM+RLMERLGNPQSKFKAIHIAGTKGKGSTAAFLS+IL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSSIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGQTGEMVSGKALNSLFKRNEEFFDQSVELENGRISY 180
           RAEGYSVGCYTSPHIETIRERISLG++GEMVSGKALN LFKRN+E  DQSV+LENGRIS+
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVFTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEV TAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIARAKAGIIKRGCPTILGGPFLPNIECILRDKALFMSSPVISASGPGNRSTIKGVSL 300
           LESIA AKAGIIK GCPTILGGPF+PNIECILRDKAL MSSPV+SAS PGNRSTIKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSTIKGVSL 300

Query: 301 LNGGLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360
           LNG LCQCCD++IQ DNEFIEL DVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI
Sbjct: 301 LNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 361 RSGLEQTFLVGRSHFLPAKEAGVLGLPGATILLDGGAFIFLFTNVKFGLLGPENCACAAS 420
           RSGLE+TFLVGRSHFL AKEAG+LGLPG TILLDG                         
Sbjct: 361 RSGLEKTFLVGRSHFLSAKEAGMLGLPGTTILLDG------------------------- 420

Query: 421 SLPYCTERLLVDVQYIGTLLAVIAHTKDSAKALVDTIQMAFPEDRLALVVAMANDKDHVG 480
                                  AHTKDSAKALVDTIQM+FP+ +LALVVAMA+DKDH G
Sbjct: 421 -----------------------AHTKDSAKALVDTIQMSFPDAQLALVVAMASDKDHNG 480

Query: 481 FAREFLRGGKLESIVLTEAHIYGGKSRTTSAAFLRDCWIQASNELGIPISLETKDALVSF 540
           FA EFL+GGKLESIVL+EA+I GGKSRTTSAA LRDCWIQASNE+GIPISLETKDA VS 
Sbjct: 481 FATEFLQGGKLESIVLSEANIGGGKSRTTSAALLRDCWIQASNEMGIPISLETKDAPVSS 540

Query: 541 TSKLENRPLLTTEPSLLHAIKIAAEILKQRTEGRRGLVIVTGSLHAVSMVLSSLHS 597
           TSKLENRP+LTTE SLL AIKIAAEILKQR EGRRGLV+VTGSLHAVSMVLSSLHS
Sbjct: 541 TSKLENRPVLTTETSLLRAIKIAAEILKQRIEGRRGLVVVTGSLHAVSMVLSSLHS 548

[Close]

BLAST of HG10010998 vs. ExPASy Swiss-Prot
Match: F4JYE9 (Dihydrofolate synthetase OS=Arabidopsis thaliana OX=3702 GN=DHFS PE=1 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 1.6e-151
Identity = 311/557 (55.83%), Postives = 381/557 (68.40%), Query Frame = 0


Query: 44  SEDQQITEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHI 103
           +ED ++ +F+ +L+SLKNYEK GVP+GAGTDSDDGFDLGRM+RLM RL NP  K+K +H+
Sbjct: 40  TEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNPHYKYKVVHV 99

Query: 104 AGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGQTGEMVSGKALNSLFKRN 163
           AGTKGKGST+AFLSNILRA GYSVGCY+SPHI +I+ERIS    GE VS   LN LF   
Sbjct: 100 AGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGEPVSASTLNDLFYSV 159

Query: 164 EEFFDQSVELENGRISYFEVFTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAA 223
           +   +QS++ ENG +S+FE+ T +AFSLF +ENVD+AVIEAGLGGARDATN+I SS LAA
Sbjct: 160 KPILEQSIQEENGSLSHFEILTGIAFSLFEKENVDIAVIEAGLGGARDATNVIESSNLAA 219

Query: 224 AVITSIGEEHMAALGGSLESIARAKAGIIKRGCPTILGGPFLPNIECILRDKALFMSSPV 283
           +VIT+IGEEHMAALGGSLESIA AK+GIIK G P +LGGPFLP+IE ILR KA  +SS V
Sbjct: 220 SVITTIGEEHMAALGGSLESIAEAKSGIIKHGRPVVLGGPFLPHIEGILRSKAASVSSSV 279

Query: 284 ISASGPGNRSTIKGVSLLNG-GLCQCCDIVIQ---IDNEFIELLDVNLRMLGRHQLQNAA 343
           I AS  G+ S+IKG+   NG GLCQ CDIVIQ    D   +EL DVNLRMLG HQLQNA 
Sbjct: 280 ILASNIGSSSSIKGIINKNGIGLCQSCDIVIQNEKDDQPIVELSDVNLRMLGHHQLQNAV 339

Query: 344 TATCVILTLRNLG-WRISDASIRSGLEQTFLVGRSHFLPAKEAGVLGLPGATILLDGGAF 403
           TATCV L LR+ G  R++D +IR GLE T L+GRS FL  KEA  L LPGAT+LLDG   
Sbjct: 340 TATCVSLCLRDQGCGRVTDEAIRIGLENTRLLGRSQFLTPKEAETLLLPGATVLLDG--- 399

Query: 404 IFLFTNVKFGLLGPENCACAASSLPYCTERLLVDVQYIGTLLAVIAHTKDSAKALVDTIQ 463
                                                        AHTK+SA+AL + I+
Sbjct: 400 ---------------------------------------------AHTKESARALKEMIK 459

Query: 464 MAFPEDRLALVVAMANDKDHVGFAREFLRGGKLESIVLTEAHIYGGKSRTTSAAFLRDCW 523
             FPE RL  VVAMA+DKDHV FA+E L G K E+++LTEA I GGK R+T ++ L++ W
Sbjct: 460 KDFPEKRLVFVVAMASDKDHVSFAKELLSGLKPEAVILTEADIGGGKIRSTESSVLKESW 519

Query: 524 IQASNELGIPISLETKDALVSFTSKLENRPLLTTEPSLLHAIKIAAEILK-QRTEGRRGL 583
           I+A++ELG   S+E            EN+       ++L ++K+A +IL    T    G+
Sbjct: 520 IKAADELG-SRSMEAS----------ENK-------TVLGSLKLAYKILSDDTTSSDSGM 528

Query: 584 VIVTGSLHAVSMVLSSL 595
           VIVTGSLH VS VL+SL
Sbjct: 580 VIVTGSLHIVSSVLASL 528

[Close]

BLAST of HG10010998 vs. ExPASy Swiss-Prot
Match: Q05865 (Dihydrofolate synthase/folylpolyglutamate synthase OS=Bacillus subtilis (strain 168) OX=224308 GN=folC PE=3 SV=2)

HSP 1 Score: 158.3 bits (399), Expect = 2.7e-37
Identity = 110/295 (37.29%), Postives = 160/295 (54.24%), Query Frame = 0


Query: 81  LGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRE 140
           LGRM++LM RLG+P+ K +A H+AGT GKGST AF+ ++L+  GY+VG +TSP+I T  E
Sbjct: 24  LGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIRSMLQEAGYTVGTFTSPYIITFNE 83

Query: 141 RISLGQTGEMVSGKALNSLFKRNEEFFDQSVELENGRISYFEVFTAMAFSLFAQ-ENVDV 200
           RIS+   G  +S +   +L  + +   +   + E G+ + FE+ TA AF  FA+   VD 
Sbjct: 84  RISV--NGIPISDEEWTALVNQMKPHVEALDQTEYGQPTEFEIMTACAFLYFAEFHKVDF 143

Query: 201 AVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGSLESIARAKAGIIKRGCPTI 260
            + E GLGG  D+TN++        VITSIG +HM  LG ++E IA  KAGIIK G P I
Sbjct: 144 VIFETGLGGRFDSTNVV---EPLLTVITSIGHDHMNILGNTIEEIAGEKAGIIKEGIP-I 203

Query: 261 LGGPFLPNIECILRDKALFMSSPVISASGPGNRSTIKGVSLLNGGLCQCCDIVIQIDNEF 320
           +     P    ++R +A   ++P  S     +   I     L  G         + + + 
Sbjct: 204 VTAVTQPEALQVIRHEAERHAAPFQSLH---DACVIFNEEALPAGE----QFSFKTEEKC 263

Query: 321 IELLDVNLRMLGRHQLQNAATATCVI--LTLRNLGWRISDASIRSGLEQTFLVGR 373
            E  D+   ++G HQ QNAA +      L   N+   ISD ++RSGL +    GR
Sbjct: 264 YE--DIRTSLIGTHQRQNAALSILAAEWLNKENIA-HISDEALRSGLVKAAWPGR 302

[Close]

BLAST of HG10010998 vs. ExPASy Swiss-Prot
Match: P15925 (Folylpolyglutamate synthase OS=Lactobacillus casei OX=1582 GN=fpgS PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 5.6e-27
Identity = 104/359 (28.97%), Postives = 162/359 (45.13%), Query Frame = 0


Query: 50  TEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGK 109
           TE + Y+ S     K G             D  R+  L+  LGNPQ + + IH+ GT GK
Sbjct: 4   TETVAYIHSFPRLAKTG-------------DHRRILTLLHALGNPQQQGRYIHVTGTNGK 63

Query: 110 GSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGQTGEMVSGKAL-NSLFKRNEEFFD 169
           GS A  ++++L A G +VG YTSP I    ERI +    E +   AL N++         
Sbjct: 64  GSAANAIAHVLEASGLTVGLYTSPFIMRFNERIMIDH--EPIPDAALVNAVAFVRAALER 123

Query: 170 QSVELENGRISYFEVFTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITS 229
              +  +  ++ FE  TA+ +  F Q  VDVAVIE G+GG  D+TN+I       +V+T 
Sbjct: 124 LQQQQADFNVTEFEFITALGYWYFRQRQVDVAVIEVGIGGDTDSTNVITP---VVSVLTE 183

Query: 230 IGEEHMAALGGSLESIARAKAGIIKRGCPTILGGPFLPNIECILRDKALFMSS------- 289
           +  +H   LG ++ +IA+ KAGIIKRG P + G   +P+   ++  K     S       
Sbjct: 184 VALDHQKLLGHTITAIAKHKAGIIKRGIPVVTGN-LVPDAAAVVAAKVATTGSQWLRFDR 243

Query: 290 ----PVISASGPGNRSTIKGVSLLNGGLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQN 349
               P     G G R T                     +++   + D+ + ++G +Q +N
Sbjct: 244 DFSVPKAKLHGWGQRFT--------------------YEDQDGRISDLEVPLVGDYQQRN 303

Query: 350 AATA-TCVILTLRNLGWRISDASIRSGLEQTFLVGRSHFLPAKEAGVLGLPGATILLDG 396
            A A     +  +   W ++  +IR GL        SH+ PA+   +   P   I++DG
Sbjct: 304 MAIAIQTAKVYAKQTEWPLTPQNIRQGL------AASHW-PARLEKISDTP--LIVIDG 314

[Close]

BLAST of HG10010998 vs. ExPASy Swiss-Prot
Match: A6H751 (Folylpolyglutamate synthase, mitochondrial OS=Bos taurus OX=9913 GN=FPGS PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 1.6e-26
Identity = 95/248 (38.31%), Postives = 127/248 (51.21%), Query Frame = 0


Query: 45  EDQQITEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLG---NPQSKFKAI 104
           E Q     +  L +  NY ++ V R  G   D    L  M+  + R G       +   I
Sbjct: 41  EYQDAVRTLNTLQTNANYLEM-VKRKRG---DPQTQLEAMKLYLARSGLQVEDLDQLNII 100

Query: 105 HIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGQTGEMVSGKALNS--- 164
           HI GTKGKGST AF   ILR+ G   G ++SPH+  +RERI +   G+ +S +       
Sbjct: 101 HITGTKGKGSTCAFTERILRSYGLKTGFFSSPHLVQVRERIRI--NGQPISSELFTKHFW 160

Query: 165 -LFKRNEEFFDQSVELENGRISYFEVFTAMAFSLFAQENVDVAVIEAGLGGARDATNIIC 224
            L+ R EE  D S  +      YF   T MAF +F QE VD+AV+E G+GGA D TNII 
Sbjct: 161 RLYHRLEETKDDSSCV--SMPGYFRFLTLMAFHVFLQEKVDLAVLEVGIGGAYDCTNII- 220

Query: 225 SSRLAAAVITSIGEEHMAALGGSLESIARAKAGIIKRGCP--TILGGPFLPNIECILRDK 284
             +     ITS+G +H   LG ++E IA  K GI K G P  T+L  P  P    +LRD+
Sbjct: 221 -RKPVVCGITSLGIDHTGLLGDTMEKIAWQKGGIFKSGVPAFTVL-QPDEP--LAVLRDR 275

[Close]

BLAST of HG10010998 vs. ExPASy Swiss-Prot
Match: Q05932 (Folylpolyglutamate synthase, mitochondrial OS=Homo sapiens OX=9606 GN=FPGS PE=1 SV=3)

HSP 1 Score: 122.5 bits (306), Expect = 1.6e-26
Identity = 96/291 (32.99%), Postives = 143/291 (49.14%), Query Frame = 0


Query: 9   RCRSQILRSLLLNHFVREWPSISTRIGSKQCFGTHSEDQQITEFMEYLDSLKNYEKLGVP 68
           R RS +  +L L         I+T++ +++        Q+ +  MEY D+++    L   
Sbjct: 3   RARSHLRAALFL--AAASARGITTQVAARRGLSAWPVPQEPS--MEYQDAVRMLNTLQTN 62

Query: 69  RG-----AGTDSDDGFDLGRMRRLMERLG---NPQSKFKAIHIAGTKGKGSTAAFLSNIL 128
            G          D    L  M   + R G       +   IH+ GTKGKGST AF   IL
Sbjct: 63  AGYLEQVKRQRGDPQTQLEAMELYLARSGLQVEDLDRLNIIHVTGTKGKGSTCAFTECIL 122

Query: 129 RAEGYSVGCYTSPHIETIRERISLGQTGEMVSGKALNSLFKRNEEFFDQSVELENGRI-- 188
           R+ G   G ++SPH+  +RERI +   G+ +S +     F R    + +  E ++G    
Sbjct: 123 RSYGLKTGFFSSPHLVQVRERIRI--NGQPISPELFTKYFWR---LYHRLEETKDGSCVS 182

Query: 189 --SYFEVFTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAA 248
              YF   T MAF +F QE VD+AV+E G+GGA D TNII   +     ++S+G +H + 
Sbjct: 183 MPPYFRFLTLMAFHVFLQEKVDLAVVEVGIGGAYDCTNII--RKPVVCGVSSLGIDHTSL 242

Query: 249 LGGSLESIARAKAGIIKRGCP--TIL--GGPFLPNIECILRDKALFMSSPV 284
           LG ++E IA  K GI K+G P  T+L   GP       +LRD+A  +S P+
Sbjct: 243 LGDTVEKIAWQKGGIFKQGVPAFTVLQPEGPL-----AVLRDRAQQISCPL 277

[Close]

BLAST of HG10010998 vs. ExPASy TrEMBL
Match: A0A1S3CNR7 (dihydrofolate synthetase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503019 PE=3 SV=1)

HSP 1 Score: 942.2 bits (2434), Expect = 1.1e-270
Identity = 494/596 (82.89%), Postives = 519/596 (87.08%), Query Frame = 0


Query: 1   MNVFKYHHRCRSQILRSLLLNHFVREWPSISTRIGSKQCFGTHSEDQQITEFMEYLDSLK 60
           MNVFKYHH CR QIL SLLLNHF+ E PSIS+R GSKQCF THSEDQ +TEFMEYLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGS AAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGQTGEMVSGKALNSLFKRNEEFFDQSVELENGRISY 180
           RAEGYSVGCYTSPHIET+RERISLG++GEMVSGKALNSLFKRN+E FDQSVELE+GRIS+
Sbjct: 121 RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH 180

Query: 181 FEVFTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEV TAMAFSLFAQENVDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIARAKAGIIKRGCPTILGGPFLPNIECILRDKALFMSSPVISASGPGNRSTIKGVSL 300
           LESIA AKAGIIKRGCPTILGG FLP IE ILRDKAL MSSPVI+ S PGNRSTIKGV++
Sbjct: 241 LESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSDPGNRSTIKGVNM 300

Query: 301 LNGGLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360
           LNGGLCQCCDIVIQIDNEF+ELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI
Sbjct: 301 LNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 361 RSGLEQTFLVGRSHFLPAKEAGVLGLPGATILLDGGAFIFLFTNVKFGLLGPENCACAAS 420
           RSGLEQTFL+GRSHFL AKEA VLGLPGATILLDG                         
Sbjct: 361 RSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDG------------------------- 420

Query: 421 SLPYCTERLLVDVQYIGTLLAVIAHTKDSAKALVDTIQMAFPEDRLALVVAMANDKDHVG 480
                                  AHTKDSAKALVDTIQMAFPE RLALV+AMA+DK+HV 
Sbjct: 421 -----------------------AHTKDSAKALVDTIQMAFPEARLALVIAMASDKNHVD 480

Query: 481 FAREFLRGGKLESIVLTEAHIYGGKSRTTSAAFLRDCWIQASNELGIPISLETKDALVSF 540
           FAREFL+GGKLE +VLTEAHI GGKSRTTSAAFLRDCWIQAS ELGIPISLETKDA VSF
Sbjct: 481 FAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRDCWIQASIELGIPISLETKDAEVSF 540

Query: 541 TSKLENRPLLTTEPSLLHAIKIAAEILKQRTEGRRGLVIVTGSLHAVSMVLSSLHS 597
           TSKLENRP+LTTE SLLHAIKIAAEILKQRT+G++ LV+V+GSLHAVSMVLSSLHS
Sbjct: 541 TSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQSLVVVSGSLHAVSMVLSSLHS 548

[Close]

BLAST of HG10010998 vs. ExPASy TrEMBL
Match: A0A6J1CJG1 (dihydrofolate synthetase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011557 PE=3 SV=1)

HSP 1 Score: 936.8 bits (2420), Expect = 4.5e-269
Identity = 495/596 (83.05%), Postives = 516/596 (86.58%), Query Frame = 0


Query: 1   MNVFKYHHRCRSQILRSLLLNHFVREWPSISTRIGSKQCFGTHSEDQQITEFMEYLDSLK 60
           MNVFKYH R R QIL  LLLN  V E PSIS RIGSKQCF THSED ++TEFMEYLDSLK
Sbjct: 1   MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGQTGEMVSGKALNSLFKRNEEFFDQSVELENGRISY 180
           RAEGYSVGCYTSPHIETIRERISLG++GEMVSGKALNSLFKRN+EF DQSVE ENGRIS+
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180

Query: 181 FEVFTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEV TAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIARAKAGIIKRGCPTILGGPFLPNIECILRDKALFMSSPVISASGPGNRSTIKGVSL 300
           LESIA AKAGIIK GCPTILGGPFLP+IECIL DKAL MSS VISAS PG+RSTIKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSL 300

Query: 301 LNGGLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360
           L+G LCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATC ILTLRNLGWRISD+SI
Sbjct: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360

Query: 361 RSGLEQTFLVGRSHFLPAKEAGVLGLPGATILLDGGAFIFLFTNVKFGLLGPENCACAAS 420
           ++GLEQTFLVGRSHFL AKEAGVLGLPGATILLDG                         
Sbjct: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDG------------------------- 420

Query: 421 SLPYCTERLLVDVQYIGTLLAVIAHTKDSAKALVDTIQMAFPEDRLALVVAMANDKDHVG 480
                                  AHTKDSAKALVDTIQM FPE RLALVVAMA+DKDHVG
Sbjct: 421 -----------------------AHTKDSAKALVDTIQMTFPEARLALVVAMASDKDHVG 480

Query: 481 FAREFLRGGKLESIVLTEAHIYGGKSRTTSAAFLRDCWIQASNELGIPISLETKDALVSF 540
           FAREFL+GGKLESI+LTEA+I GGKSRTTSAA LRDCWIQA+NELGIPISLETKDALVS 
Sbjct: 481 FAREFLQGGKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIPISLETKDALVSS 540

Query: 541 TSKLENRPLLTTEPSLLHAIKIAAEILKQRTEGRRGLVIVTGSLHAVSMVLSSLHS 597
           TSKLE+RP+LTTE SLLHA+KIAAEILKQRTEGR GL +VTGSLHAVSMVLSSLHS
Sbjct: 541 TSKLESRPILTTETSLLHAMKIAAEILKQRTEGRAGLTVVTGSLHAVSMVLSSLHS 548

[Close]

BLAST of HG10010998 vs. ExPASy TrEMBL
Match: A0A0A0L7A4 (Mur_ligase_M domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G354540 PE=3 SV=1)

HSP 1 Score: 935.3 bits (2416), Expect = 1.3e-268
Identity = 490/596 (82.21%), Postives = 519/596 (87.08%), Query Frame = 0


Query: 1   MNVFKYHHRCRSQILRSLLLNHFVREWPSISTRIGSKQCFGTHSEDQQITEFMEYLDSLK 60
           MNVFKYHH CR QIL SLLLNHF+ E PSIS+R GSKQCF THSEDQ +T+FMEYLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRG+GTDSDDGFDLGRMRRLMERLGNPQS+FKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGQTGEMVSGKALNSLFKRNEEFFDQSVELENGRISY 180
           R EGYSVGCYTSPHIETIRERISLG++G+MVSGKALNSLFKRN+E FDQSVELENG +S+
Sbjct: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180

Query: 181 FEVFTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEV TAMAFSLFAQE+VDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEH+AALGGS
Sbjct: 181 FEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGS 240

Query: 241 LESIARAKAGIIKRGCPTILGGPFLPNIECILRDKALFMSSPVISASGPGNRSTIKGVSL 300
           LESIA AKAGIIKRGCPTILGGPFLP IE ILRDKAL MSSPVISAS PGNRSTIKGV+L
Sbjct: 241 LESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNL 300

Query: 301 LNGGLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360
           LNGGL QCCDIVIQIDNEFIELLDVNLRMLG HQLQNAATATCVILTLRNLGWRISDASI
Sbjct: 301 LNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 361 RSGLEQTFLVGRSHFLPAKEAGVLGLPGATILLDGGAFIFLFTNVKFGLLGPENCACAAS 420
           RSGLEQTFL+GRSHFL A+EA VLGLPGATILLDG                         
Sbjct: 361 RSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDG------------------------- 420

Query: 421 SLPYCTERLLVDVQYIGTLLAVIAHTKDSAKALVDTIQMAFPEDRLALVVAMANDKDHVG 480
                                  AHTKDSAKAL+DTIQMAFPE +LALVVAMA+DK+HVG
Sbjct: 421 -----------------------AHTKDSAKALLDTIQMAFPEAQLALVVAMASDKNHVG 480

Query: 481 FAREFLRGGKLESIVLTEAHIYGGKSRTTSAAFLRDCWIQASNELGIPISLETKDALVSF 540
           FAREFL+GGKLES+VLTEA I GGKSRTTSAAFLRDCWIQASNELGIPISLETKDA V F
Sbjct: 481 FAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRDCWIQASNELGIPISLETKDAEVFF 540

Query: 541 TSKLENRPLLTTEPSLLHAIKIAAEILKQRTEGRRGLVIVTGSLHAVSMVLSSLHS 597
           TSKL NRP+LTTE SLLHAIKIAAEILKQRT+GR+GLV+V+GSLHAVSMVL+SLHS
Sbjct: 541 TSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGRQGLVVVSGSLHAVSMVLASLHS 548

[Close]

BLAST of HG10010998 vs. ExPASy TrEMBL
Match: A0A6J1E819 (dihydrofolate synthetase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430739 PE=3 SV=1)

HSP 1 Score: 917.9 bits (2371), Expect = 2.1e-263
Identity = 481/596 (80.70%), Postives = 511/596 (85.74%), Query Frame = 0


Query: 1   MNVFKYHHRCRSQILRSLLLNHFVREWPSISTRIGSKQCFGTHSEDQQITEFMEYLDSLK 60
           MN+FKYHH  R QI   LLLN+FV E PSIS+RIGSKQCF  HSEDQ +TEFMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFRNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGTDS+DGFDLGRM+RLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGQTGEMVSGKALNSLFKRNEEFFDQSVELENGRISY 180
           RAEGYSVGCYTSPHIETIRERISLG++GEMVSGKALN LFKRN+E  DQSV+LENGRIS+
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVFTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEV TAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIARAKAGIIKRGCPTILGGPFLPNIECILRDKALFMSSPVISASGPGNRSTIKGVSL 300
           LESIA AKAGIIK GCPTILGGPF+PNIECILRDKAL MSSPV+SAS PGNRS+IKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSSIKGVSL 300

Query: 301 LNGGLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360
           LNG LCQCCD++IQ DNEFIEL DVNLRMLGRHQLQNAATATCVILTLRNLGWRIS+ASI
Sbjct: 301 LNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISEASI 360

Query: 361 RSGLEQTFLVGRSHFLPAKEAGVLGLPGATILLDGGAFIFLFTNVKFGLLGPENCACAAS 420
           RSGLE+TFLVGRSHFL AKEAG+LGLPG  ILLDG                         
Sbjct: 361 RSGLEKTFLVGRSHFLSAKEAGILGLPGTAILLDG------------------------- 420

Query: 421 SLPYCTERLLVDVQYIGTLLAVIAHTKDSAKALVDTIQMAFPEDRLALVVAMANDKDHVG 480
                                  AHTKDSAKALVDTIQM+FP+ +LALVVAMA+DKDH G
Sbjct: 421 -----------------------AHTKDSAKALVDTIQMSFPDAQLALVVAMASDKDHYG 480

Query: 481 FAREFLRGGKLESIVLTEAHIYGGKSRTTSAAFLRDCWIQASNELGIPISLETKDALVSF 540
           FA EFL+GGKLESIVL+EA+I G KSRTTSAA LRDCWIQASNE+GIPISLETKDA VS 
Sbjct: 481 FATEFLQGGKLESIVLSEANIGGSKSRTTSAALLRDCWIQASNEMGIPISLETKDAPVSS 540

Query: 541 TSKLENRPLLTTEPSLLHAIKIAAEILKQRTEGRRGLVIVTGSLHAVSMVLSSLHS 597
           TSKLENRP+LTTE SLL AIKIAAEILKQR +GRRGLV+VTGSLHAVSMVLSSLHS
Sbjct: 541 TSKLENRPVLTTETSLLRAIKIAAEILKQRIDGRRGLVVVTGSLHAVSMVLSSLHS 548

[Close]

BLAST of HG10010998 vs. ExPASy TrEMBL
Match: A0A6J1J6Z7 (dihydrofolate synthetase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483133 PE=3 SV=1)

HSP 1 Score: 885.9 bits (2288), Expect = 9.0e-254
Identity = 471/596 (79.03%), Postives = 497/596 (83.39%), Query Frame = 0


Query: 1   MNVFKYHHRCRSQILRSLLLNHFVREWPSISTRIGSKQCFGTHSEDQQITEFMEYLDSLK 60
           MNVFKYHH  R QIL  LLLN+FV E PSIS+RIGSKQCFG HSEDQQ+TEFMEYLDSLK
Sbjct: 1   MNVFKYHHHFRLQILGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQQMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGTDS+DGFDLGRM+RLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGQTGEMVSGKALNSLFKRNEEFFDQSVELENGRISY 180
           RAEGYSVGCYTSPHIETIRERISLG++GEMVSGKALN LFKRN+E  DQSV+LENGRIS+
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVFTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEV TAMAFSLFAQENVDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSILAAAVITSIGEEHMAALGGS 240

Query: 241 LESIARAKAGIIKRGCPTILGGPFLPNIECILRDKALFMSSPVISASGPGNRSTIKGVSL 300
           LESIA AKAGIIK GCPTILGGPF+PNIECILRDKAL MSSPV+SAS PGNRSTIKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSTIKGVSL 300

Query: 301 LNGGLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360
           LNG LCQCCD++IQ D+EFIEL DVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI
Sbjct: 301 LNGRLCQCCDLIIQTDDEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 361 RSGLEQTFLVGRSHFLPAKEAGVLGLPGATILLDGGAFIFLFTNVKFGLLGPENCACAAS 420
           RSGLE+TFLVGRSHFL AKEAG+LGLPG TILLDG                         
Sbjct: 361 RSGLEKTFLVGRSHFLSAKEAGMLGLPGTTILLDG------------------------- 420

Query: 421 SLPYCTERLLVDVQYIGTLLAVIAHTKDSAKALVDTIQMAFPEDRLALVVAMANDKDHVG 480
                                  AHTKDSAKALVDTIQM+FP+ +LALVVAMA+DKDH G
Sbjct: 421 -----------------------AHTKDSAKALVDTIQMSFPDAQLALVVAMASDKDHNG 480

Query: 481 FAREFLRGGKLESIVLTEAHIYGGKSRTTSAAFLRDCWIQASNELGIPISLETKDALVSF 540
           FA EFL+ GKLESIVL+EA+I GGKSRTTSAA LRDCWIQASNE+GIP            
Sbjct: 481 FATEFLQCGKLESIVLSEANIGGGKSRTTSAALLRDCWIQASNEMGIPTQTS-------- 532

Query: 541 TSKLENRPLLTTEPSLLHAIKIAAEILKQRTEGRRGLVIVTGSLHAVSMVLSSLHS 597
                   LLTTE SLL AIKIAAEILKQRTEGRRGLV+VTGSLHAVSMVLSSLHS
Sbjct: 541 --------LLTTETSLLRAIKIAAEILKQRTEGRRGLVVVTGSLHAVSMVLSSLHS 532

[Close]

BLAST of HG10010998 vs. TAIR 10
Match: AT5G41480.1 (Folylpolyglutamate synthetase family protein )

HSP 1 Score: 537.7 bits (1384), Expect = 1.2e-152
Identity = 311/557 (55.83%), Postives = 381/557 (68.40%), Query Frame = 0


Query: 44  SEDQQITEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHI 103
           +ED ++ +F+ +L+SLKNYEK GVP+GAGTDSDDGFDLGRM+RLM RL NP  K+K +H+
Sbjct: 40  TEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNPHYKYKVVHV 99

Query: 104 AGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGQTGEMVSGKALNSLFKRN 163
           AGTKGKGST+AFLSNILRA GYSVGCY+SPHI +I+ERIS    GE VS   LN LF   
Sbjct: 100 AGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGEPVSASTLNDLFYSV 159

Query: 164 EEFFDQSVELENGRISYFEVFTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAA 223
           +   +QS++ ENG +S+FE+ T +AFSLF +ENVD+AVIEAGLGGARDATN+I SS LAA
Sbjct: 160 KPILEQSIQEENGSLSHFEILTGIAFSLFEKENVDIAVIEAGLGGARDATNVIESSNLAA 219

Query: 224 AVITSIGEEHMAALGGSLESIARAKAGIIKRGCPTILGGPFLPNIECILRDKALFMSSPV 283
           +VIT+IGEEHMAALGGSLESIA AK+GIIK G P +LGGPFLP+IE ILR KA  +SS V
Sbjct: 220 SVITTIGEEHMAALGGSLESIAEAKSGIIKHGRPVVLGGPFLPHIEGILRSKAASVSSSV 279

Query: 284 ISASGPGNRSTIKGVSLLNG-GLCQCCDIVIQ---IDNEFIELLDVNLRMLGRHQLQNAA 343
           I AS  G+ S+IKG+   NG GLCQ CDIVIQ    D   +EL DVNLRMLG HQLQNA 
Sbjct: 280 ILASNIGSSSSIKGIINKNGIGLCQSCDIVIQNEKDDQPIVELSDVNLRMLGHHQLQNAV 339

Query: 344 TATCVILTLRNLG-WRISDASIRSGLEQTFLVGRSHFLPAKEAGVLGLPGATILLDGGAF 403
           TATCV L LR+ G  R++D +IR GLE T L+GRS FL  KEA  L LPGAT+LLDG   
Sbjct: 340 TATCVSLCLRDQGCGRVTDEAIRIGLENTRLLGRSQFLTPKEAETLLLPGATVLLDG--- 399

Query: 404 IFLFTNVKFGLLGPENCACAASSLPYCTERLLVDVQYIGTLLAVIAHTKDSAKALVDTIQ 463
                                                        AHTK+SA+AL + I+
Sbjct: 400 ---------------------------------------------AHTKESARALKEMIK 459

Query: 464 MAFPEDRLALVVAMANDKDHVGFAREFLRGGKLESIVLTEAHIYGGKSRTTSAAFLRDCW 523
             FPE RL  VVAMA+DKDHV FA+E L G K E+++LTEA I GGK R+T ++ L++ W
Sbjct: 460 KDFPEKRLVFVVAMASDKDHVSFAKELLSGLKPEAVILTEADIGGGKIRSTESSVLKESW 519

Query: 524 IQASNELGIPISLETKDALVSFTSKLENRPLLTTEPSLLHAIKIAAEILK-QRTEGRRGL 583
           I+A++ELG   S+E            EN+       ++L ++K+A +IL    T    G+
Sbjct: 520 IKAADELG-SRSMEAS----------ENK-------TVLGSLKLAYKILSDDTTSSDSGM 528

Query: 584 VIVTGSLHAVSMVLSSL 595
           VIVTGSLH VS VL+SL
Sbjct: 580 VIVTGSLHIVSSVLASL 528

[Close]

BLAST of HG10010998 vs. TAIR 10
Match: AT3G55630.3 (DHFS-FPGS homolog D )

HSP 1 Score: 115.2 bits (287), Expect = 1.9e-25
Identity = 92/297 (30.98%), Postives = 135/297 (45.45%), Query Frame = 0


Query: 96  SKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGQTGEMVSGKA 155
           S+ K IH+AGTKGKGST  F  +ILR  G   G +TSPH+  +RER  L   G  +S + 
Sbjct: 56  SQMKIIHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRL--NGIEISQEK 115

Query: 156 LNSLFKRNEEFFDQSVELENGRISYFEVFTAMAFSLFAQENVDVAVIEAGLGGARDATNI 215
             + F        +    E    +YF     +AF +F  E VDV ++E GLGG  DATN+
Sbjct: 116 FVNYFWCCFHKLKEKTSNEVPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNV 175

Query: 216 ICSSRLAAAVITSIGEEHMAALGGSLESIARAKAGIIKRGCPTILGGPFLPNIECILRDK 275
           I   +     I+S+G +HM  LG +L  IA  KAGI K G P                  
Sbjct: 176 I--QKPVVCGISSLGYDHMEILGYTLAEIAAEKAGIFKSGVP------------------ 235

Query: 276 ALFMSSPVISASGPGNRSTIKGVSLLNGGLCQCCDIVIQIDNEFIELLDVNLR--MLGRH 335
           A  ++ P             + + +LN    +     ++++ + +E LD + R  + G H
Sbjct: 236 AFTVAQP------------DEAMRVLNEKASK-----LEVNLQVVEPLDSSQRLGLQGEH 295

Query: 336 QLQNAATATCVILT-LRNLGWRISDA---------SIRSGLEQTFLVGRSHFLPAKE 381
           Q  NA  A  +  T L+ +G    +             SGL   +L+GR+  +P  E
Sbjct: 296 QYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMIVPDSE 313

[Close]

BLAST of HG10010998 vs. TAIR 10
Match: AT3G55630.2 (DHFS-FPGS homolog D )

HSP 1 Score: 113.6 bits (283), Expect = 5.4e-25
Identity = 92/297 (30.98%), Postives = 134/297 (45.12%), Query Frame = 0


Query: 96  SKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGQTGEMVSGKA 155
           S+ K IH+AGTKGKGST  F  +ILR  G   G +TSPH+  +RER  L   G  +S + 
Sbjct: 56  SQMKIIHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRL--NGIEISQEK 115

Query: 156 LNSLFKRNEEFFDQSVELENGRISYFEVFTAMAFSLFAQENVDVAVIEAGLGGARDATNI 215
             + F        +    E    +YF     +AF +F  E VDV ++E GLGG  DATNI
Sbjct: 116 FVNYFWCCFHKLKEKTSNEVPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNI 175

Query: 216 ICSSRLAAAVITSIGEEHMAALGGSLESIARAKAGIIKRGCPTILGGPFLPNIECILRDK 275
               +     I+S+G +HM  LG +L  IA  KAGI K G P                  
Sbjct: 176 ---QKPVVCGISSLGYDHMEILGYTLAEIAAEKAGIFKSGVP------------------ 235

Query: 276 ALFMSSPVISASGPGNRSTIKGVSLLNGGLCQCCDIVIQIDNEFIELLDVNLR--MLGRH 335
           A  ++ P             + + +LN    +     ++++ + +E LD + R  + G H
Sbjct: 236 AFTVAQP------------DEAMRVLNEKASK-----LEVNLQVVEPLDSSQRLGLQGEH 295

Query: 336 QLQNAATATCVILT-LRNLGWRISDA---------SIRSGLEQTFLVGRSHFLPAKE 381
           Q  NA  A  +  T L+ +G    +             SGL   +L+GR+  +P  E
Sbjct: 296 QYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMIVPDSE 312

[Close]

BLAST of HG10010998 vs. TAIR 10
Match: AT5G05980.1 (DHFS-FPGS homolog B )

HSP 1 Score: 112.8 bits (281), Expect = 9.2e-25
Identity = 86/258 (33.33%), Postives = 120/258 (46.51%), Query Frame = 0


Query: 10  CRSQILRSLLLNHFVREWPSISTRIGSKQCFGTHSEDQQITEFME----YLDSLKNYEKL 69
           C+  I  +L L+H    +      + S   F  HS  +QI    +    Y ++L     L
Sbjct: 21  CQLSIPLTLRLHH---HYQHHQPHLPSPLSFQIHSLRKQIDMAAQGGDSYEEALAALSSL 80

Query: 70  GVPRGAGTDSDDG------FDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNI 129
              R     S+ G      FD  ++  L E +     K   IH+AGTKGKGST  F  +I
Sbjct: 81  ITKRSRADKSNKGDRFELVFDYLKLLDLEEDI----LKMNVIHVAGTKGKGSTCTFTESI 140

Query: 130 LRAEGYSVGCYTSPHIETIRERISLGQTGEMVSGKALNSLFKRNEEFFDQSVELENGRIS 189
           +R  G+  G +TSPH+  +RER  L   G  +S +     F        +    E    +
Sbjct: 141 IRNYGFRTGLFTSPHLIDVRERFRL--DGVDISEEKFLGYFWWCYNRLKERTNEEIPMPT 200

Query: 190 YFEVFTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGG 249
           YF     +AF +FA E VD A++E GLGG  DATN +   +     I+S+G +HM  LG 
Sbjct: 201 YFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAV--QKPVVCGISSLGYDHMEILGD 260

Query: 250 SLESIARAKAGIIKRGCP 258
           +L  IA  KAGI K G P
Sbjct: 261 TLGKIAGEKAGIFKLGVP 267

[Close]

BLAST of HG10010998 vs. TAIR 10
Match: AT5G05980.2 (DHFS-FPGS homolog B )

HSP 1 Score: 109.4 bits (272), Expect = 1.0e-23
Identity = 65/161 (40.37%), Postives = 86/161 (53.42%), Query Frame = 0


Query: 97  KFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGQTGEMVSGKAL 156
           K   IH+AGTKGKGST  F  +I+R  G+  G +TSPH+  +RER  L   G  +S +  
Sbjct: 53  KMNVIHVAGTKGKGSTCTFTESIIRNYGFRTGLFTSPHLIDVRERFRL--DGVDISEEKF 112

Query: 157 NSLFKRNEEFFDQSVELENGRISYFEVFTAMAFSLFAQENVDVAVIEAGLGGARDATNII 216
              F        +    E    +YF     +AF +FA E VD A++E GLGG  DATN +
Sbjct: 113 LGYFWWCYNRLKERTNEEIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAV 172

Query: 217 CSSRLAAAVITSIGEEHMAALGGSLESIARAKAGIIKRGCP 258
              +     I+S+G +HM  LG +L  IA  KAGI K G P
Sbjct: 173 --QKPVVCGISSLGYDHMEILGDTLGKIAGEKAGIFKLGVP 209

The following BLAST results are available for this feature:

BLAST of HG10010998 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905573.1	2.3e-275	83.89	dihydrofolate synthetase [Benincasa hispida]	[more]
XP_008465388.1	2.2e-270	82.89	PREDICTED: dihydrofolate synthetase isoform X1 [Cucumis melo]	[more]
XP_022141068.1	9.2e-269	83.05	dihydrofolate synthetase isoform X1 [Momordica charantia]	[more]
XP_004149457.1	2.7e-268	82.21	dihydrofolate synthetase isoform X2 [Cucumis sativus]	[more]
XP_023553540.1	4.3e-266	81.71	dihydrofolate synthetase [Cucurbita pepo subsp. pepo]	[more]

BLAST of HG10010998 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JYE9	1.6e-151	55.83	Dihydrofolate synthetase OS=Arabidopsis thaliana OX=3702 GN=DHFS PE=1 SV=1	[more]
Q05865	2.7e-37	37.29	Dihydrofolate synthase/folylpolyglutamate synthase OS=Bacillus subtilis (strain ...	[more]
P15925	5.6e-27	28.97	Folylpolyglutamate synthase OS=Lactobacillus casei OX=1582 GN=fpgS PE=1 SV=1	[more]
A6H751	1.6e-26	38.31	Folylpolyglutamate synthase, mitochondrial OS=Bos taurus OX=9913 GN=FPGS PE=2 SV...	[more]
Q05932	1.6e-26	32.99	Folylpolyglutamate synthase, mitochondrial OS=Homo sapiens OX=9606 GN=FPGS PE=1 ...	[more]

BLAST of HG10010998 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CNR7	1.1e-270	82.89	dihydrofolate synthetase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503019 PE=3...	[more]
A0A6J1CJG1	4.5e-269	83.05	dihydrofolate synthetase isoform X1 OS=Momordica charantia OX=3673 GN=LOC1110115...	[more]
A0A0A0L7A4	1.3e-268	82.21	Mur_ligase_M domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G35454...	[more]
A0A6J1E819	2.1e-263	80.70	dihydrofolate synthetase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11143073...	[more]
A0A6J1J6Z7	9.0e-254	79.03	dihydrofolate synthetase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483133 ...	[more]

BLAST of HG10010998 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G41480.1	1.2e-152	55.83	Folylpolyglutamate synthetase family protein	[more]
AT3G55630.3	1.9e-25	30.98	DHFS-FPGS homolog D	[more]
AT3G55630.2	5.4e-25	30.98	DHFS-FPGS homolog D	[more]
AT5G05980.1	9.2e-25	33.33	DHFS-FPGS homolog B	[more]
AT5G05980.2	1.0e-23	40.37	DHFS-FPGS homolog B	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013221	Mur ligase, central	PFAM	PF08245	Mur_ligase_M	coord: 103..254 e-value: 1.7E-8 score: 34.6
IPR036615	Mur ligase, C-terminal domain superfamily	GENE3D	3.90.190.20		coord: 436..596 e-value: 1.9E-13 score: 52.8
IPR036615	Mur ligase, C-terminal domain superfamily	SUPERFAMILY	53244	MurD-like peptide ligases, peptide-binding domain	coord: 444..594
IPR036565	Mur-like, catalytic domain superfamily	GENE3D	3.40.1190.10		coord: 38..368 e-value: 8.1E-86 score: 289.7
IPR036565	Mur-like, catalytic domain superfamily	SUPERFAMILY	53623	MurD-like peptide ligases, catalytic domain	coord: 78..368
IPR001645	Folylpolyglutamate synthetase	TIGRFAM	TIGR01499	TIGR01499	coord: 81..396 e-value: 3.7E-76 score: 255.0
IPR001645	Folylpolyglutamate synthetase	PANTHER	PTHR11136	FOLYLPOLYGLUTAMATE SYNTHASE-RELATED	coord: 46..396 coord: 443..594
None	No IPR available	PANTHER	PTHR11136:SF0	DIHYDROFOLATE SYNTHETASE-RELATED	coord: 46..396 coord: 443..594
IPR018109	Folylpolyglutamate synthetase, conserved site	PROSITE	PS01011	FOLYLPOLYGLU_SYNT_1	coord: 101..124
IPR018109	Folylpolyglutamate synthetase, conserved site	PROSITE	PS01012	FOLYLPOLYGLU_SYNT_2	coord: 201..216

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10010998.1	HG10010998.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006761	dihydrofolate biosynthetic process
biological_process	GO:0009793	embryo development ending in seed dormancy
biological_process	GO:0046901	tetrahydrofolylpolyglutamate biosynthetic process
biological_process	GO:0009058	biosynthetic process
biological_process	GO:0009396	folic acid-containing compound biosynthetic process
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005759	mitochondrial matrix
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0008841	dihydrofolate synthase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004326	tetrahydrofolylpolyglutamate synthase activity
molecular_function	GO:0016874	ligase activity