HG10010656 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10010656
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionPyruvate dehydrogenase E1 component subunit alpha
LocationChr06: 24498966 .. 24501453 (-)
RNA-Seq ExpressionHG10010656
SyntenyHG10010656
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCTTCTCTGCATCACTCAAAATCCTCCAACCCCTTCCTCTCAACTCCACCAGATCCAATGAAAAACCCCTCTTCTTTGATCCCTTTAGATCCCCCTCCAAGTTTCTTGGATCTCGATTTCGCATCCCTTCTCTCTCTAAATCCAATACTCGCTCTCGATCCTCCCCTGCCGTTGCCGTTTCCGATGTTGTCAAGGAGAAGAAGCTCAAACCCAGTTCCAATATGGTACTCTTTCGAATTCTAGATGATGGATTTGTTGGGTTGATTGATTTGTTAATGGGTTTCTAATGTTATGGAAATCATGGGAGAATCGGGTAATGTAGCATGAAAATTTCGCTTTGTATGCCCGCATGGAATCATCTAGCTTTAACGATTTTGCTTTTGTTGGTTTTTTAATTTGTTGATTTTGGTCTCCCTGTTGTTGTTTTCTTTTGGAAATTTAGATGGTTTGAGATTGTCATTATGAAATTGGAGGTTATTATTTGTTCTTTTGCCCCACCAGTGGAATTAGCTTTGTTGAATTATTGAATTCGTGCAAAATTTGACTCTACTGGAAGCTTCTGTATGGCTTTGCTACATGAACTATTTTGTTTAAAAGTTCTTCCCTTTTTTTTTTGGTTCCCTTTATTGTCTCCACACAGCTGATTACCAAAGAAGAGGGTTTGGTACTCTACGAGGACATGATATTAGGCAGAGAGTTTGAGGATATGTGTGCACAGATGTATTACAGAGGCAAAATGTTTGGTTTTGTTCACCTTTACAATGGCCAAGAAGCTGTTTCCACCGGGTTCATCAAGCTTCTTACAAAGAGGGATACTGTAGTAAGCACTTATCGTGATCACGTTCATGCTCTCAGCAAGGGAGTTCCATCCCGTGAGGTCATGAGTGAGCTTTTTGGAAAGACCACTGGTTGCTGCCGTGGCCAAGGTGGCTCGATGCATATGTTCTCAAAAGAACATAATCTGATAGGTGGCTTTGCCTTCATCGGGGAAGGCATACCAGTGGCCACTGGTGCTGCATTCACATCTAAATACAAGAGAGAAGTTTTAAAAGAGGCAGACTGTGAAGATGTCACACTGGCGTTTTTCGGAGATGGGACTTGCAACAATGGCCAGTTCTTTGAGTGTTTGAACATGGCAGCTCTGTGGAAATTGCCAATTGTATTTGTTGTGGAGAATAATCTTTGGGCAATTGGAATGTCACATTTGAGGGCTACTTCAGATCCTGAGATTTGGAAGAAGGGTCCTGCATTTGGAATGCCTGGCGTTCATGTTGATGGTATGGATGTTTTGAAGGTGAGGGAGGTTGCAAAGGAGGCAATTGGAAGAGCCAGGAGAGGAGAAGGACCGACTCTCGTGGAATGCGAAACCTATAGGTTTAGAGGACACTCACTGGCTGATCCAGATGAACTCCGCGACCCTGGTTAGTGCTCTTAAATTCATACCTACATTTGGTTCTGTCATTTCTAAATATGATTTTGCTTGGCCTTGTTGACATTTATTATTACGGCTAGTCAAAGTTCCTTAATTCATTTCTCATGAATCATGATAGCCAATCTCTTAATCCGTACTCCATCATAGCATTTAAGGATAATTCATTCAGGGTTGTAATGAGCTGCACCACTATAAATTCACATAGTTATCTCATTTGTTCATTTATCATTTTTTATGAACAAAGAGGGAAATGAGTGTTATAGTTGATTTTACCAGTATTTAATCCAGAGTGCATTATCTGAGGCCAGATTACATTGTTCAATCTTCAAATATCAGTCTACTGGATACAATTAGTGGACTCATCTTGCAATGTTTACTCTATAGATTCTAAGTGAACCAAACATCATAGAAGTGGAAGTGACAACTGGTTTTTTCTTTTATTTCAAGTGAACATTGTAAAAGGAAGAAACCAGTCAAACTTGATGGACATAACTACTCCATTATGTGACTTTTATATGATTGCTCACAAGGAAAAACACTTGGAACTCATGTGATATATATTTAGCAAATTGGCATGATAATAATATGTAGATTTCTAATGTGGTGTCTTTAGAGTTCATGGACAGACTCGATTAGATGATAGAACTCAATGTCCCCATCAGGTTCTTCATTGTACTTTGCTTACTTACACACACAAATGTAGTAGTTTCTTATCCAAGTATAATAACTCTAGAAAGTACAAAGGATTAAGAATTCCCGATTTAACCGTGGCAGACGAGAAGGCCCGTTATGCTGCTAGAGATCCCATTGCAGCTTTGAAGAAATACTTGCTGGAGAATAATCTAGCCAGTGAACAGGAGCTAAAAGGGATAAAGGACAAGATAGTTGAGGTGGTTGAGGATGCAGTTAGTTTTGCAGATGAAAGTCCTCTTCCAGCCAGAAGCCAGTTATTAGAGAATGTGTTTGCTGATCCTAAAGGCTTTGGAATTGGGCCTGATGGAAAGTACAGATGTGAGGATCCCAAGTTCACAGAAGGCACTGCACATGTCTAA

mRNA sequence

ATGTCCTTCTCTGCATCACTCAAAATCCTCCAACCCCTTCCTCTCAACTCCACCAGATCCAATGAAAAACCCCTCTTCTTTGATCCCTTTAGATCCCCCTCCAAGTTTCTTGGATCTCGATTTCGCATCCCTTCTCTCTCTAAATCCAATACTCGCTCTCGATCCTCCCCTGCCGTTGCCGTTTCCGATGTTGTCAAGGAGAAGAAGCTCAAACCCAGTTCCAATATGCTGATTACCAAAGAAGAGGGTTTGGTACTCTACGAGGACATGATATTAGGCAGAGAGTTTGAGGATATGTGTGCACAGATGTATTACAGAGGCAAAATGTTTGGTTTTGTTCACCTTTACAATGGCCAAGAAGCTGTTTCCACCGGGTTCATCAAGCTTCTTACAAAGAGGGATACTGTAGTAAGCACTTATCGTGATCACGTTCATGCTCTCAGCAAGGGAGTTCCATCCCGTGAGGTCATGAGTGAGCTTTTTGGAAAGACCACTGGTTGCTGCCGTGGCCAAGGTGGCTCGATGCATATGTTCTCAAAAGAACATAATCTGATAGGTGGCTTTGCCTTCATCGGGGAAGGCATACCAGTGGCCACTGGTGCTGCATTCACATCTAAATACAAGAGAGAAGTTTTAAAAGAGGCAGACTGTGAAGATGTCACACTGGCGTTTTTCGGAGATGGGACTTGCAACAATGGCCAGTTCTTTGAGTGTTTGAACATGGCAGCTCTGTGGAAATTGCCAATTGTATTTGTTGTGGAGAATAATCTTTGGGCAATTGGAATGTCACATTTGAGGGCTACTTCAGATCCTGAGATTTGGAAGAAGGGTCCTGCATTTGGAATGCCTGGCGTTCATGTTGATGGTATGGATGTTTTGAAGGTGAGGGAGGTTGCAAAGGAGGCAATTGGAAGAGCCAGGAGAGGAGAAGGACCGACTCTCGTGGAATGCGAAACCTATAGGTTTAGAGGACACTCACTGGCTGATCCAGATGAACTCCGCGACCCTGACGAGAAGGCCCGTTATGCTGCTAGAGATCCCATTGCAGCTTTGAAGAAATACTTGCTGGAGAATAATCTAGCCAGTGAACAGGAGCTAAAAGGGATAAAGGACAAGATAGTTGAGGTGGTTGAGGATGCAGTTAGTTTTGCAGATGAAAGTCCTCTTCCAGCCAGAAGCCAGTTATTAGAGAATGTGTTTGCTGATCCTAAAGGCTTTGGAATTGGGCCTGATGGAAAGTACAGATGTGAGGATCCCAAGTTCACAGAAGGCACTGCACATGTCTAA

Coding sequence (CDS)

ATGTCCTTCTCTGCATCACTCAAAATCCTCCAACCCCTTCCTCTCAACTCCACCAGATCCAATGAAAAACCCCTCTTCTTTGATCCCTTTAGATCCCCCTCCAAGTTTCTTGGATCTCGATTTCGCATCCCTTCTCTCTCTAAATCCAATACTCGCTCTCGATCCTCCCCTGCCGTTGCCGTTTCCGATGTTGTCAAGGAGAAGAAGCTCAAACCCAGTTCCAATATGCTGATTACCAAAGAAGAGGGTTTGGTACTCTACGAGGACATGATATTAGGCAGAGAGTTTGAGGATATGTGTGCACAGATGTATTACAGAGGCAAAATGTTTGGTTTTGTTCACCTTTACAATGGCCAAGAAGCTGTTTCCACCGGGTTCATCAAGCTTCTTACAAAGAGGGATACTGTAGTAAGCACTTATCGTGATCACGTTCATGCTCTCAGCAAGGGAGTTCCATCCCGTGAGGTCATGAGTGAGCTTTTTGGAAAGACCACTGGTTGCTGCCGTGGCCAAGGTGGCTCGATGCATATGTTCTCAAAAGAACATAATCTGATAGGTGGCTTTGCCTTCATCGGGGAAGGCATACCAGTGGCCACTGGTGCTGCATTCACATCTAAATACAAGAGAGAAGTTTTAAAAGAGGCAGACTGTGAAGATGTCACACTGGCGTTTTTCGGAGATGGGACTTGCAACAATGGCCAGTTCTTTGAGTGTTTGAACATGGCAGCTCTGTGGAAATTGCCAATTGTATTTGTTGTGGAGAATAATCTTTGGGCAATTGGAATGTCACATTTGAGGGCTACTTCAGATCCTGAGATTTGGAAGAAGGGTCCTGCATTTGGAATGCCTGGCGTTCATGTTGATGGTATGGATGTTTTGAAGGTGAGGGAGGTTGCAAAGGAGGCAATTGGAAGAGCCAGGAGAGGAGAAGGACCGACTCTCGTGGAATGCGAAACCTATAGGTTTAGAGGACACTCACTGGCTGATCCAGATGAACTCCGCGACCCTGACGAGAAGGCCCGTTATGCTGCTAGAGATCCCATTGCAGCTTTGAAGAAATACTTGCTGGAGAATAATCTAGCCAGTGAACAGGAGCTAAAAGGGATAAAGGACAAGATAGTTGAGGTGGTTGAGGATGCAGTTAGTTTTGCAGATGAAAGTCCTCTTCCAGCCAGAAGCCAGTTATTAGAGAATGTGTTTGCTGATCCTAAAGGCTTTGGAATTGGGCCTGATGGAAAGTACAGATGTGAGGATCCCAAGTTCACAGAAGGCACTGCACATGTCTAA

Protein sequence

MSFSASLKILQPLPLNSTRSNEKPLFFDPFRSPSKFLGSRFRIPSLSKSNTRSRSSPAVAVSDVVKEKKLKPSSNMLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENNLASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPKFTEGTAHV
Homology
BLAST of HG10010656 vs. NCBI nr
Match: XP_038875269.1 (pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Benincasa hispida])

HSP 1 Score: 860.9 bits (2223), Expect = 4.6e-246
Identity = 423/428 (98.83%), Postives = 426/428 (99.53%), Query Frame = 0

Query: 1   MSFSASLKILQPLPLNSTRSNEKPLFFDPFRSPSKFLGSRFRIPSLSKSNTRSRSSPAVA 60
           MSFS+SLKILQPLPLNSTRSNEKPLFFDPFRS SKFLGSRFRIPSLSKSNTRSRSSPAVA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNEKPLFFDPFRSSSKFLGSRFRIPSLSKSNTRSRSSPAVA 60

Query: 61  VSDVVKEKKLKPSSNMLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSN+LITKEEGLVLYEDM+LGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENNL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPI ALKKYLLENNL
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIVALKKYLLENNL 360

Query: 361 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 429
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

BLAST of HG10010656 vs. NCBI nr
Match: XP_008459830.1 (PREDICTED: pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucumis melo] >KAA0039805.1 pyruvate dehydrogenase E1 component subunit alpha-3 [Cucumis melo var. makuwa] >TYK24692.1 pyruvate dehydrogenase E1 component subunit alpha-3 [Cucumis melo var. makuwa])

HSP 1 Score: 842.8 bits (2176), Expect = 1.3e-240
Identity = 412/428 (96.26%), Postives = 420/428 (98.13%), Query Frame = 0

Query: 1   MSFSASLKILQPLPLNSTRSNEKPLFFDPFRSPSKFLGSRFRIPSLSKSNTRSRSSPAVA 60
           MSFS+SLKILQPLPLNSTRSN+KPL FDPFRS SKFLGSRFR+PSLSKSNTR RSSP VA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPTVA 60

Query: 61  VSDVVKEKKLKPSSNMLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSN+LITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLT+RDTVVSTYRDHVHA+SKGVPSR+VMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTQRDTVVSTYRDHVHAISKGVPSRQVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKE DCEDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKETDCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENNL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLEN L
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL 360

Query: 361 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           A+EQELK IKDKIVEVVEDAV FADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ANEQELKAIKDKIVEVVEDAVQFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 429
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

BLAST of HG10010656 vs. NCBI nr
Match: XP_004140530.1 (pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucumis sativus] >KGN46471.1 hypothetical protein Csa_005756 [Cucumis sativus])

HSP 1 Score: 840.1 bits (2169), Expect = 8.4e-240
Identity = 411/428 (96.03%), Postives = 420/428 (98.13%), Query Frame = 0

Query: 1   MSFSASLKILQPLPLNSTRSNEKPLFFDPFRSPSKFLGSRFRIPSLSKSNTRSRSSPAVA 60
           MSFS+SLKILQPLPLNSTRSN+KPL FDPFRS SKFLGSRFR+PSLSKSNTR RSSP VA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNMLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSN+LITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLT+RDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADC+DVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENNL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKY+LEN L
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360

Query: 361 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           A+EQELK IKDKIVEVVE+AV FADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 429
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

BLAST of HG10010656 vs. NCBI nr
Match: KAG6575304.1 (Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 811.2 bits (2094), Expect = 4.2e-231
Identity = 400/428 (93.46%), Postives = 409/428 (95.56%), Query Frame = 0

Query: 1   MSFSASLKILQPLPLNSTRSNEKPLFFDPFRSPSKFLGSRFRIPSLSKSNTRSRSSPAVA 60
           MS SAS KILQPL LNS RSN+KPLFFDPFRS S FLGSRFR PS+SKS TRSRSSP VA
Sbjct: 1   MSLSASPKILQPLLLNSPRSNQKPLFFDPFRSTSNFLGSRFRFPSISKSTTRSRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNMLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLK  SN+LITKEEGLVLYEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKSGSNLLITKEEGLVLYEDMILGRVFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVP+REVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPAREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           EHN+IGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNVIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENNL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDP EKARYAARDPIAALKKYL+E NL
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPAEKARYAARDPIAALKKYLVEKNL 360

Query: 361 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           ASEQELK I+ +I EVVE+AV FADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ASEQELKAIEKRIDEVVEEAVEFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 429
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

BLAST of HG10010656 vs. NCBI nr
Match: XP_022992260.1 (pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucurbita maxima])

HSP 1 Score: 810.1 bits (2091), Expect = 9.3e-231
Identity = 399/428 (93.22%), Postives = 408/428 (95.33%), Query Frame = 0

Query: 1   MSFSASLKILQPLPLNSTRSNEKPLFFDPFRSPSKFLGSRFRIPSLSKSNTRSRSSPAVA 60
           MSFSAS KILQPL LNS RSNEKPLFFDPFRS S FLGSRFR PS+SKS TR RSSP VA
Sbjct: 1   MSFSASPKILQPLLLNSPRSNEKPLFFDPFRSTSNFLGSRFRFPSISKSTTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNMLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLK  SN+LITKEEGLV+YEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKSGSNLLITKEEGLVVYEDMILGRVFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVP+REVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPAREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           EHN+IGGFAFIGEGIPVATGAAFTSKYKREVLKE DCEDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNVIGGFAFIGEGIPVATGAAFTSKYKREVLKEGDCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENNL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDP EKARYAARDPIAALKKYL+E NL
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPAEKARYAARDPIAALKKYLVEKNL 360

Query: 361 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           ASEQELK I+ +I EVVE+AV FADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ASEQELKAIEKRIGEVVEEAVEFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 429
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

BLAST of HG10010656 vs. ExPASy Swiss-Prot
Match: O24457 (Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PDH-E1 ALPHA PE=2 SV=1)

HSP 1 Score: 677.9 bits (1748), Expect = 7.3e-194
Identity = 341/428 (79.67%), Postives = 372/428 (86.92%), Query Frame = 0

Query: 5   ASLKILQPLPLNSTRSNEKPLFFDPFR--SPSKFLGSRFRIPSLSKSN--TRSRSSPAVA 64
           A  K+   +PL+ +  N   L   P R   PS FLGS  R  SL + N    +R SP V+
Sbjct: 6   APTKLTATVPLHGSHENRLLL---PIRLAPPSSFLGST-RSLSLRRLNHSNATRRSPVVS 65

Query: 65  VSDVVKEKKLKPSSNMLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 124
           V +VVKEK+   ++++LITKEEGL LYEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 66  VQEVVKEKQSTNNTSLLITKEEGLELYEDMILGRSFEDMCAQMYYRGKMFGFVHLYNGQE 125

Query: 125 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 184
           AVSTGFIKLLTK D+VVSTYRDHVHALSKGV +R VMSELFGK TGCCRGQGGSMHMFSK
Sbjct: 126 AVSTGFIKLLTKSDSVVSTYRDHVHALSKGVSARAVMSELFGKVTGCCRGQGGSMHMFSK 185

Query: 185 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 244
           EHN++GGFAFIGEGIPVATGAAF+SKY+REVLK+ DC+DVT+AFFGDGTCNNGQFFECLN
Sbjct: 186 EHNMLGGFAFIGEGIPVATGAAFSSKYRREVLKQ-DCDDVTVAFFGDGTCNNGQFFECLN 245

Query: 245 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 304
           MAAL+KLPI+FVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 246 MAALYKLPIIFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 305

Query: 305 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENNL 364
           EA+ RARRGEGPTLVECETYRFRGHSLADPDELRD  EKA+YAARDPIAALKKYL+EN L
Sbjct: 306 EAVTRARRGEGPTLVECETYRFRGHSLADPDELRDAAEKAKYAARDPIAALKKYLIENKL 365

Query: 365 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 424
           A E ELK I+ KI E+VE+AV FAD SP P RSQLLENVFADPKGFGIGPDG+YRCEDPK
Sbjct: 366 AKEAELKSIEKKIDELVEEAVEFADASPQPGRSQLLENVFADPKGFGIGPDGRYRCEDPK 425

Query: 425 FTEGTAHV 429
           FTEGTA V
Sbjct: 426 FTEGTAQV 428

BLAST of HG10010656 vs. ExPASy Swiss-Prot
Match: Q7XTJ3 (Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0119400 PE=2 SV=2)

HSP 1 Score: 635.6 bits (1638), Expect = 4.1e-181
Identity = 322/431 (74.71%), Postives = 357/431 (82.83%), Query Frame = 0

Query: 2   SFSASLKILQPLPLNSTRSNEKPLFFDPFRSPSKFLGSRFRIPSLSKSNTRSRSSPAVAV 61
           SF+A+ K L P+   S    + PL   P  + +     R       K   R R++ AV+ 
Sbjct: 6   SFTAAAKFLAPVSARSAGDYKPPL---PLPASASLRPGR-------KPAPRLRTALAVS- 65

Query: 62  SDVVKEKKLKP--SSNMLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQ 121
           SDV+   K  P  +++  +T+EE L LYEDM+LGR FEDMCAQMYYRGKMFGFVHLYNGQ
Sbjct: 66  SDVLPGNKAAPAAAAHSAVTREEALELYEDMVLGRIFEDMCAQMYYRGKMFGFVHLYNGQ 125

Query: 122 EAVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFS 181
           EAVSTGFIKLL + D VVSTYRDHVHALSKGVP+R VM+ELFGK TGCCRGQGGSMHMFS
Sbjct: 126 EAVSTGFIKLLNQADCVVSTYRDHVHALSKGVPARSVMAELFGKATGCCRGQGGSMHMFS 185

Query: 182 KEHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEA--DCEDVTLAFFGDGTCNNGQFFE 241
           + HNL+GGFAFIGEGIPVATGAAF +KY+ EVLK++  D  DVTLAFFGDGTCNNGQFFE
Sbjct: 186 EPHNLLGGFAFIGEGIPVATGAAFAAKYRHEVLKQSSPDGLDVTLAFFGDGTCNNGQFFE 245

Query: 242 CLNMAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVRE 301
           CLNMA LWKLPIVFVVENNLWAIGMSHLRATSDPEI+KKGPAFGMPGVHVDGMDVLKVRE
Sbjct: 246 CLNMAQLWKLPIVFVVENNLWAIGMSHLRATSDPEIYKKGPAFGMPGVHVDGMDVLKVRE 305

Query: 302 VAKEAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLE 361
           VAKEAI RARRGEGPTLVECETYRFRGHSLADPDELR PDEK+ YAARDPI ALKKY++E
Sbjct: 306 VAKEAIERARRGEGPTLVECETYRFRGHSLADPDELRRPDEKSHYAARDPITALKKYIIE 365

Query: 362 NNLASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCE 421
            NLA+E ELK I+ KI +VVE+AV FAD SPLP RSQLLENVF+DPKGFGIGPDGKYRCE
Sbjct: 366 QNLATESELKSIEKKIDDVVEEAVEFADASPLPPRSQLLENVFSDPKGFGIGPDGKYRCE 425

Query: 422 DPKFTEGTAHV 429
           DP FT+GTA V
Sbjct: 426 DPLFTQGTAQV 425

BLAST of HG10010656 vs. ExPASy Swiss-Prot
Match: Q1XDM0 (Pyruvate dehydrogenase E1 component subunit alpha OS=Pyropia yezoensis OX=2788 GN=pdhA PE=3 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 1.4e-128
Identity = 223/330 (67.58%), Postives = 261/330 (79.09%), Query Frame = 0

Query: 73  SSNMLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTK 132
           S+ + + K   LVLYEDM+LGR FEDMCAQMYY+GKMFGFVHLYNGQEAVSTG IKLL  
Sbjct: 16  STGLNLNKSNLLVLYEDMLLGRNFEDMCAQMYYKGKMFGFVHLYNGQEAVSTGVIKLLNP 75

Query: 133 RDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIG 192
            D V STYRDHVHALSKGVPS+ VM+ELFGK TGC +G+GGSMH+FS  HN +GGFAFI 
Sbjct: 76  TDYVCSTYRDHVHALSKGVPSKNVMAELFGKETGCSKGRGGSMHIFSAPHNFLGGFAFIA 135

Query: 193 EGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFV 252
           EGIPVATGAAF S Y+++VLKE +   VT  FFGDGT NNGQFFECLNMA LWKLPI+FV
Sbjct: 136 EGIPVATGAAFQSIYRQQVLKETEDLRVTACFFGDGTTNNGQFFECLNMAVLWKLPIIFV 195

Query: 253 VENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGP 312
           VENN WAIGM+H R++S PEI KK  AFG+PG+ VDGMDVL VR+ AK+A+ RAR+G+GP
Sbjct: 196 VENNQWAIGMAHHRSSSIPEIHKKAEAFGLPGIEVDGMDVLAVRQAAKQAVQRARQGDGP 255

Query: 313 TLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENNLASEQELKGIKDK 372
           TL+E  TYRFRGHSLADPDELR   EK  + ARDPI  LKKY+L+N +A+  EL  I++ 
Sbjct: 256 TLIEALTYRFRGHSLADPDELRSRQEKEAWVARDPIKKLKKYILDNEIANIGELNEIQNA 315

Query: 373 IVEVVEDAVSFADESPLPARSQLLENVFAD 403
           +   +E AV FA  SP P  S+L   +FAD
Sbjct: 316 VKTELEQAVKFAISSPEPNMSELKRYLFAD 345

BLAST of HG10010656 vs. ExPASy Swiss-Prot
Match: P51267 (Pyruvate dehydrogenase E1 component subunit alpha OS=Porphyra purpurea OX=2787 GN=pdhA PE=3 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 4.1e-128
Identity = 225/336 (66.96%), Postives = 264/336 (78.57%), Query Frame = 0

Query: 67  EKKLKPSSNMLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGF 126
           E  L   + + +TK + LVLYEDM+LGR FEDMCAQMYY+GKMFGFVHLYNGQEAVSTG 
Sbjct: 8   ELPLTNCNQINLTKHKLLVLYEDMLLGRNFEDMCAQMYYKGKMFGFVHLYNGQEAVSTGV 67

Query: 127 IKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIG 186
           IKLL  +D V STYRDHVHALSKGVPS+ VM+ELFGK TGC RG+GGSMH+FS  HN +G
Sbjct: 68  IKLLDSKDYVCSTYRDHVHALSKGVPSQNVMAELFGKETGCSRGRGGSMHIFSAPHNFLG 127

Query: 187 GFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWK 246
           GFAFI EGIPVATGAAF S Y+++VLKE     VT  FFGDGT NNGQFFECLNMA LWK
Sbjct: 128 GFAFIAEGIPVATGAAFQSIYRQQVLKEPGELRVTACFFGDGTTNNGQFFECLNMAVLWK 187

Query: 247 LPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRA 306
           LPI+FVVENN WAIGM+H R++S PEI KK  AFG+PG+ VDGMDVL VR+VA++A+ RA
Sbjct: 188 LPIIFVVENNQWAIGMAHHRSSSIPEIHKKAEAFGLPGIEVDGMDVLAVRQVAEKAVERA 247

Query: 307 RRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENNLASEQEL 366
           R+G+GPTL+E  TYRFRGHSLADPDELR   EK  + ARDPI  LKK++L+N +AS  EL
Sbjct: 248 RQGQGPTLIEALTYRFRGHSLADPDELRSRQEKEAWVARDPIKKLKKHILDNQIASSDEL 307

Query: 367 KGIKDKIVEVVEDAVSFADESPLPARSQLLENVFAD 403
             I+  +   +E +V FA  SP P  S+L   +FAD
Sbjct: 308 NDIQSSVKIDLEQSVEFAMSSPEPNISELKRYLFAD 343

BLAST of HG10010656 vs. ExPASy Swiss-Prot
Match: Q4UKQ6 (Pyruvate dehydrogenase E1 component subunit alpha OS=Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) OX=315456 GN=pdhA PE=3 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 4.7e-76
Identity = 135/336 (40.18%), Postives = 207/336 (61.61%), Query Frame = 0

Query: 65  VKEKKLKPSSNMLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVST 124
           +K KK KP      TKEE +  ++DM+L R FE+ C Q+Y  G++ GF HLY GQEAV +
Sbjct: 3   IKPKKYKP------TKEEYIKSFKDMLLLRRFEEKCGQLYGMGEIGGFCHLYIGQEAVIS 62

Query: 125 GFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNL 184
               +  K D+ +++YRDH H +  G   + V++EL G+ TGC +G+GGSMH+F   +  
Sbjct: 63  AVDTVKQKGDSTITSYRDHAHIILAGTEPKYVLAELMGRATGCSKGKGGSMHLFDIPNKF 122

Query: 185 IGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAAL 244
            GG   +G  +P+ TG AF  KY           ++   F GDG  N GQ +E  NMAAL
Sbjct: 123 YGGHGIVGAQVPIGTGLAFAEKYN-------GTNNICFTFLGDGAVNQGQVYEAFNMAAL 182

Query: 245 WKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIG 304
           W LP+V+++ENN +++G S  R+T   +++KKG +FG+ G  +DGMD  ++   AK+A  
Sbjct: 183 WGLPVVYIIENNEYSMGTSVARSTFMRDLYKKGESFGIKGFQLDGMDFEEMYNGAKQAAE 242

Query: 305 RARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENNLASEQ 364
             R    P ++E +TYR+RGHS++DP + R  +E  +Y  RDP+  ++K +L+N  A+E 
Sbjct: 243 YVRENSFPLILEVKTYRYRGHSMSDPAKYRSKEEVEQYKERDPLVIIRKTILDNKYATEA 302

Query: 365 ELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVF 401
           +LK I+  + E+V++AV F++ SPLP   +L   V+
Sbjct: 303 DLKEIEQSVKEIVKEAVKFSENSPLPDEGELYTEVY 325

BLAST of HG10010656 vs. ExPASy TrEMBL
Match: A0A5A7TEC1 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G002450 PE=4 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 6.3e-241
Identity = 412/428 (96.26%), Postives = 420/428 (98.13%), Query Frame = 0

Query: 1   MSFSASLKILQPLPLNSTRSNEKPLFFDPFRSPSKFLGSRFRIPSLSKSNTRSRSSPAVA 60
           MSFS+SLKILQPLPLNSTRSN+KPL FDPFRS SKFLGSRFR+PSLSKSNTR RSSP VA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPTVA 60

Query: 61  VSDVVKEKKLKPSSNMLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSN+LITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLT+RDTVVSTYRDHVHA+SKGVPSR+VMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTQRDTVVSTYRDHVHAISKGVPSRQVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKE DCEDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKETDCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENNL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLEN L
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL 360

Query: 361 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           A+EQELK IKDKIVEVVEDAV FADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ANEQELKAIKDKIVEVVEDAVQFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 429
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

BLAST of HG10010656 vs. ExPASy TrEMBL
Match: A0A1S3CB66 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo OX=3656 GN=LOC103498837 PE=4 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 6.3e-241
Identity = 412/428 (96.26%), Postives = 420/428 (98.13%), Query Frame = 0

Query: 1   MSFSASLKILQPLPLNSTRSNEKPLFFDPFRSPSKFLGSRFRIPSLSKSNTRSRSSPAVA 60
           MSFS+SLKILQPLPLNSTRSN+KPL FDPFRS SKFLGSRFR+PSLSKSNTR RSSP VA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPTVA 60

Query: 61  VSDVVKEKKLKPSSNMLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSN+LITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLT+RDTVVSTYRDHVHA+SKGVPSR+VMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTQRDTVVSTYRDHVHAISKGVPSRQVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKE DCEDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKETDCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENNL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLEN L
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL 360

Query: 361 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           A+EQELK IKDKIVEVVEDAV FADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ANEQELKAIKDKIVEVVEDAVQFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 429
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

BLAST of HG10010656 vs. ExPASy TrEMBL
Match: A0A0A0KFF3 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis sativus OX=3659 GN=Csa_6G095870 PE=4 SV=1)

HSP 1 Score: 840.1 bits (2169), Expect = 4.1e-240
Identity = 411/428 (96.03%), Postives = 420/428 (98.13%), Query Frame = 0

Query: 1   MSFSASLKILQPLPLNSTRSNEKPLFFDPFRSPSKFLGSRFRIPSLSKSNTRSRSSPAVA 60
           MSFS+SLKILQPLPLNSTRSN+KPL FDPFRS SKFLGSRFR+PSLSKSNTR RSSP VA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNMLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSN+LITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLT+RDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADC+DVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENNL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKY+LEN L
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360

Query: 361 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           A+EQELK IKDKIVEVVE+AV FADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 429
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

BLAST of HG10010656 vs. ExPASy TrEMBL
Match: A0A6J1JV75 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita maxima OX=3661 GN=LOC111488634 PE=4 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 4.5e-231
Identity = 399/428 (93.22%), Postives = 408/428 (95.33%), Query Frame = 0

Query: 1   MSFSASLKILQPLPLNSTRSNEKPLFFDPFRSPSKFLGSRFRIPSLSKSNTRSRSSPAVA 60
           MSFSAS KILQPL LNS RSNEKPLFFDPFRS S FLGSRFR PS+SKS TR RSSP VA
Sbjct: 1   MSFSASPKILQPLLLNSPRSNEKPLFFDPFRSTSNFLGSRFRFPSISKSTTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNMLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLK  SN+LITKEEGLV+YEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKSGSNLLITKEEGLVVYEDMILGRVFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVP+REVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPAREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           EHN+IGGFAFIGEGIPVATGAAFTSKYKREVLKE DCEDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNVIGGFAFIGEGIPVATGAAFTSKYKREVLKEGDCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENNL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDP EKARYAARDPIAALKKYL+E NL
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPAEKARYAARDPIAALKKYLVEKNL 360

Query: 361 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           ASEQELK I+ +I EVVE+AV FADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ASEQELKAIEKRIGEVVEEAVEFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 429
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

BLAST of HG10010656 vs. ExPASy TrEMBL
Match: A0A6J1EPB1 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita moschata OX=3662 GN=LOC111436336 PE=4 SV=1)

HSP 1 Score: 808.1 bits (2086), Expect = 1.7e-230
Identity = 399/428 (93.22%), Postives = 407/428 (95.09%), Query Frame = 0

Query: 1   MSFSASLKILQPLPLNSTRSNEKPLFFDPFRSPSKFLGSRFRIPSLSKSNTRSRSSPAVA 60
           MS SAS KILQPL LNS RS EKPLFFDPFRS S FLGSRFR PS+SKS TR RSSP VA
Sbjct: 1   MSLSASPKILQPLLLNSPRSTEKPLFFDPFRSTSNFLGSRFRFPSISKSTTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNMLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLK  SN+LITKEEGLVLYEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKSGSNLLITKEEGLVLYEDMILGRVFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVP+REVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPAREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           EHN+IGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNVIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENNL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDP EKARYAARDPIAALKKYL+E NL
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPAEKARYAARDPIAALKKYLVEKNL 360

Query: 361 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           ASEQELK I+ +I EVVE+AV FADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ASEQELKAIEKRIDEVVEEAVEFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 429
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

BLAST of HG10010656 vs. TAIR 10
Match: AT1G01090.1 (pyruvate dehydrogenase E1 alpha )

HSP 1 Score: 677.9 bits (1748), Expect = 5.2e-195
Identity = 341/428 (79.67%), Postives = 372/428 (86.92%), Query Frame = 0

Query: 5   ASLKILQPLPLNSTRSNEKPLFFDPFR--SPSKFLGSRFRIPSLSKSN--TRSRSSPAVA 64
           A  K+   +PL+ +  N   L   P R   PS FLGS  R  SL + N    +R SP V+
Sbjct: 6   APTKLTATVPLHGSHENRLLL---PIRLAPPSSFLGST-RSLSLRRLNHSNATRRSPVVS 65

Query: 65  VSDVVKEKKLKPSSNMLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 124
           V +VVKEK+   ++++LITKEEGL LYEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 66  VQEVVKEKQSTNNTSLLITKEEGLELYEDMILGRSFEDMCAQMYYRGKMFGFVHLYNGQE 125

Query: 125 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 184
           AVSTGFIKLLTK D+VVSTYRDHVHALSKGV +R VMSELFGK TGCCRGQGGSMHMFSK
Sbjct: 126 AVSTGFIKLLTKSDSVVSTYRDHVHALSKGVSARAVMSELFGKVTGCCRGQGGSMHMFSK 185

Query: 185 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 244
           EHN++GGFAFIGEGIPVATGAAF+SKY+REVLK+ DC+DVT+AFFGDGTCNNGQFFECLN
Sbjct: 186 EHNMLGGFAFIGEGIPVATGAAFSSKYRREVLKQ-DCDDVTVAFFGDGTCNNGQFFECLN 245

Query: 245 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 304
           MAAL+KLPI+FVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 246 MAALYKLPIIFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 305

Query: 305 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENNL 364
           EA+ RARRGEGPTLVECETYRFRGHSLADPDELRD  EKA+YAARDPIAALKKYL+EN L
Sbjct: 306 EAVTRARRGEGPTLVECETYRFRGHSLADPDELRDAAEKAKYAARDPIAALKKYLIENKL 365

Query: 365 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 424
           A E ELK I+ KI E+VE+AV FAD SP P RSQLLENVFADPKGFGIGPDG+YRCEDPK
Sbjct: 366 AKEAELKSIEKKIDELVEEAVEFADASPQPGRSQLLENVFADPKGFGIGPDGRYRCEDPK 425

Query: 425 FTEGTAHV 429
           FTEGTA V
Sbjct: 426 FTEGTAQV 428

BLAST of HG10010656 vs. TAIR 10
Match: AT1G59900.1 (pyruvate dehydrogenase complex E1 alpha subunit )

HSP 1 Score: 261.2 bits (666), Expect = 1.5e-69
Identity = 143/348 (41.09%), Postives = 205/348 (58.91%), Query Frame = 0

Query: 72  PSSNMLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMF-GFVHLYNGQEAVSTGFIKLL 131
           PS ++  + +E L  +  M L R  E + A   Y+ K+  GF HLY+GQEAV+ G    +
Sbjct: 48  PSRSVESSSQELLDFFRTMALMRRME-IAADSLYKAKLIRGFCHLYDGQEAVAIGMEAAI 107

Query: 132 TKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAF 191
           TK+D +++ YRDH   L +G    EV SEL G+  GC +G+GGSMH + KE +  GG   
Sbjct: 108 TKKDAIITAYRDHCIFLGRGGSLHEVFSELMGRQAGCSKGKGGSMHFYKKESSFYGGHGI 167

Query: 192 IGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIV 251
           +G  +P+  G AF  KY +E       E VT A +GDG  N GQ FE LN++ALW LP +
Sbjct: 168 VGAQVPLGCGIAFAQKYNKE-------EAVTFALYGDGAANQGQLFEALNISALWDLPAI 227

Query: 252 FVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGE 311
            V ENN + +G +  RA   P  +K+G    +PG+ VDGMD   V++  K A   A   +
Sbjct: 228 LVCENNHYGMGTAEWRAAKSPSYYKRGDY--VPGLKVDGMDAFAVKQACKFAKQHALE-K 287

Query: 312 GPTLVECETYRFRGHSLADP-DELRDPDE-KARYAARDPIAALKKYLLENNLASEQELKG 371
           GP ++E +TYR+ GHS++DP    R  DE       RDPI  +KK +L ++LA+E+ELK 
Sbjct: 288 GPIILEMDTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERIKKLVLSHDLATEKELKD 347

Query: 372 IKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFG---IGPDGK 414
           ++ +I + V+DA++ A + P+P  S+L  NV+   KGFG    GPD K
Sbjct: 348 MEKEIRKEVDDAIAKAKDCPMPEPSELFTNVYV--KGFGTESFGPDRK 382

BLAST of HG10010656 vs. TAIR 10
Match: AT1G24180.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 252.3 bits (643), Expect = 7.0e-67
Identity = 144/377 (38.20%), Postives = 215/377 (57.03%), Query Frame = 0

Query: 43  IPSLSKSNTRSRSSPAVAVSDVVKEKKL--KPSSNMLITKEEGLVLYEDMILGREFEDMC 102
           +PS  + +  + SSP    + V     L   PS ++  + EE L  + DM   R  E + 
Sbjct: 21  LPSSIRRHVSTDSSPITIETAVPFTSHLCESPSRSVETSSEEILAFFRDMARMRRME-IA 80

Query: 103 AQMYYRGKMF-GFVHLYNGQEAVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSE 162
           A   Y+ K+  GF HLY+GQEA++ G    +TK+D ++++YRDH   + +G    +  SE
Sbjct: 81  ADSLYKAKLIRGFCHLYDGQEALAVGMEAAITKKDAIITSYRDHCTFIGRGGKLVDAFSE 140

Query: 163 LFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCED 222
           L G+ TGC  G+GGSMH + K+ +  GG   +G  IP+  G AF  KY ++       E 
Sbjct: 141 LMGRKTGCSHGKGGSMHFYKKDASFYGGHGIVGAQIPLGCGLAFAQKYNKD-------EA 200

Query: 223 VTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPA 282
           VT A +GDG  N GQ FE LN++ALW LP + V ENN + +G +  R+   P  +K+G  
Sbjct: 201 VTFALYGDGAANQGQLFEALNISALWDLPAILVCENNHYGMGTATWRSAKSPAYFKRGDY 260

Query: 283 FGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVECETYRFRGHSLADP-DELRDPDE 342
             +PG+ VDGMD L V++  K A   A +  GP ++E +TYR+ GHS++DP    R  DE
Sbjct: 261 --VPGLKVDGMDALAVKQACKFAKEHALK-NGPIILEMDTYRYHGHSMSDPGSTYRTRDE 320

Query: 343 -KARYAARDPIAALKKYLLENNLASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLE 402
                  RDPI  ++K LL +++A+E+ELK ++ +I + V+DAV+ A ESP+P  S+L  
Sbjct: 321 ISGVRQVRDPIERVRKLLLTHDIATEKELKDMEKEIRKEVDDAVAQAKESPIPDASELFT 380

Query: 403 NVFADPKGF-GIGPDGK 414
           N++    G    G D K
Sbjct: 381 NMYVKDCGVESFGADRK 386

BLAST of HG10010656 vs. TAIR 10
Match: AT5G09300.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 125.2 bits (313), Expect = 1.3e-28
Identity = 84/327 (25.69%), Postives = 158/327 (48.32%), Query Frame = 0

Query: 78  ITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTVV 137
           +++E  + +Y DM+  +  +++  +   +G++  F     G+EA++      LT +D + 
Sbjct: 125 VSEEVAVKIYSDMVTLQIMDNIFYEAQRQGRL-SFYATAIGEEAINIASAAALTPQDVIF 184

Query: 138 STYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIPV 197
             YR+    L +G   +E  ++ FG  +   +G+   +H  S + N     A I   +P 
Sbjct: 185 PQYREPGVLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPN 244

Query: 198 ATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNL 257
           A GAA++ K  ++       +   + +FGDG  + G F   LN+AA+ + P++F+  NN 
Sbjct: 245 AVGAAYSLKMDKK-------DACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNG 304

Query: 258 WAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVEC 317
           WAI            +  KG A+G+  + VDG D L +      A   A R + P L+E 
Sbjct: 305 WAISTPTSDQFRSDGVVVKGRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEA 364

Query: 318 ETYRFRGHSLADPD-ELRDPDEKARY-AARDPIAALKKYLLENNLASEQELKGIKDKIVE 377
            TYR   HS +D     R   E   +  AR+P++  + ++  N   S++    ++ +I +
Sbjct: 365 LTYRVGHHSTSDDSTRYRSAGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKK 424

Query: 378 VVEDAVSFADESPLPARSQLLENVFAD 403
            + +A+  A+++  P     L+N+F+D
Sbjct: 425 EMLEALRVAEKTEKPN----LQNMFSD 439

BLAST of HG10010656 vs. TAIR 10
Match: AT5G09300.2 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 125.2 bits (313), Expect = 1.3e-28
Identity = 84/327 (25.69%), Postives = 158/327 (48.32%), Query Frame = 0

Query: 78  ITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTVV 137
           +++E  + +Y DM+  +  +++  +   +G++  F     G+EA++      LT +D + 
Sbjct: 54  VSEEVAVKIYSDMVTLQIMDNIFYEAQRQGRL-SFYATAIGEEAINIASAAALTPQDVIF 113

Query: 138 STYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIPV 197
             YR+    L +G   +E  ++ FG  +   +G+   +H  S + N     A I   +P 
Sbjct: 114 PQYREPGVLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPN 173

Query: 198 ATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNL 257
           A GAA++ K  ++       +   + +FGDG  + G F   LN+AA+ + P++F+  NN 
Sbjct: 174 AVGAAYSLKMDKK-------DACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNG 233

Query: 258 WAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVEC 317
           WAI            +  KG A+G+  + VDG D L +      A   A R + P L+E 
Sbjct: 234 WAISTPTSDQFRSDGVVVKGRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEA 293

Query: 318 ETYRFRGHSLADPD-ELRDPDEKARY-AARDPIAALKKYLLENNLASEQELKGIKDKIVE 377
            TYR   HS +D     R   E   +  AR+P++  + ++  N   S++    ++ +I +
Sbjct: 294 LTYRVGHHSTSDDSTRYRSAGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKK 353

Query: 378 VVEDAVSFADESPLPARSQLLENVFAD 403
            + +A+  A+++  P     L+N+F+D
Sbjct: 354 EMLEALRVAEKTEKPN----LQNMFSD 368

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038875269.14.6e-24698.83pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Benincasa hi... [more]
XP_008459830.11.3e-24096.26PREDICTED: pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [C... [more]
XP_004140530.18.4e-24096.03pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucumis sati... [more]
KAG6575304.14.2e-23193.46Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic, partial [Cuc... [more]
XP_022992260.19.3e-23193.22pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucurbita ma... [more]
Match NameE-valueIdentityDescription
O244577.3e-19479.67Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic OS=Arabidopsi... [more]
Q7XTJ34.1e-18174.71Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic OS=Oryza sati... [more]
Q1XDM01.4e-12867.58Pyruvate dehydrogenase E1 component subunit alpha OS=Pyropia yezoensis OX=2788 G... [more]
P512674.1e-12866.96Pyruvate dehydrogenase E1 component subunit alpha OS=Porphyra purpurea OX=2787 G... [more]
Q4UKQ64.7e-7640.18Pyruvate dehydrogenase E1 component subunit alpha OS=Rickettsia felis (strain AT... [more]
Match NameE-valueIdentityDescription
A0A5A7TEC16.3e-24196.26Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo var. makuwa OX... [more]
A0A1S3CB666.3e-24196.26Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A0A0KFF34.1e-24096.03Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis sativus OX=3659 GN=... [more]
A0A6J1JV754.5e-23193.22Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1EPB11.7e-23093.22Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita moschata OX=3662 ... [more]
Match NameE-valueIdentityDescription
AT1G01090.15.2e-19579.67pyruvate dehydrogenase E1 alpha [more]
AT1G59900.11.5e-6941.09pyruvate dehydrogenase complex E1 alpha subunit [more]
AT1G24180.17.0e-6738.20Thiamin diphosphate-binding fold (THDP-binding) superfamily protein [more]
AT5G09300.11.3e-2825.69Thiamin diphosphate-binding fold (THDP-binding) superfamily protein [more]
AT5G09300.21.3e-2825.69Thiamin diphosphate-binding fold (THDP-binding) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.970coord: 43..410
e-value: 1.7E-121
score: 407.7
NoneNo IPR availablePANTHERPTHR11516:SF58PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHAcoord: 5..428
NoneNo IPR availablePANTHERPTHR11516PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLARcoord: 5..428
NoneNo IPR availableCDDcd02000TPP_E1_PDC_ADC_BCADCcoord: 86..384
e-value: 2.85714E-152
score: 431.149
IPR017597Pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit, subgroup yTIGRFAMTIGR03182TIGR03182coord: 81..401
e-value: 1.5E-139
score: 462.2
IPR001017Dehydrogenase, E1 componentPFAMPF00676E1_dhcoord: 89..390
e-value: 4.3E-80
score: 268.9
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 75..403

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10010656.1HG10010656.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006086 acetyl-CoA biosynthetic process from pyruvate
biological_process GO:0006096 glycolytic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0004739 pyruvate dehydrogenase (acetyl-transferring) activity
molecular_function GO:0016624 oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor