HG10010533 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10010533 vs. NCBI nr
Match: XP_038906710.1 (uncharacterized protein LOC120092637 [Benincasa hispida])

HSP 1 Score: 1007.3 bits (2603), Expect = 5.2e-290
Identity = 523/563 (92.90%), Postives = 540/563 (95.91%), Query Frame = 0

Query: 1   MDNGCGIAKTICSICYEDLKPIIEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC 60
           MDNGCG AKTICSICYEDLKPIIEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC
Sbjct: 1   MDNGCGFAKTICSICYEDLKPIIEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC 60

Query: 61  KQTCVGKDVARLYFQSVGDANDSMLAKKPADWVEKDPELLRSEVKRLEMKVSVLNSALET 120
           KQTC+GKDVARLYFQSVGDANDSMLAKKPADWVEKDPELLRSEVKRLEMKVSVLNS LET
Sbjct: 61  KQTCMGKDVARLYFQSVGDANDSMLAKKPADWVEKDPELLRSEVKRLEMKVSVLNSTLET 120

Query: 121 QGKDLKELHEELQLCQDELKKEVGLRNDALKQKTSMSQLLHLKSEELEKSTVECKRLHER 180
           QGKDLKELHEELQLCQDELKKEVGLRNDA+KQKTSMSQLLHLK+EELEKSTVECKRLHER
Sbjct: 121 QGKDLKELHEELQLCQDELKKEVGLRNDAMKQKTSMSQLLHLKTEELEKSTVECKRLHER 180

Query: 181 TMSLAKELAAFKLVSDVDLDEEEVLKLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAK 240
           +M+LAKELAAFKLVS+VDLDEEEVLKLSSLG GADNKD IDILRKSLVLRNRSYKELMAK
Sbjct: 181 SMALAKELAAFKLVSEVDLDEEEVLKLSSLGGGADNKDAIDILRKSLVLRNRSYKELMAK 240

Query: 241 CNLLGRGEARSCKKLEKANEKINKLKVRVQELEKAIEDKDNEVLRGLKDANKQGVQGSVC 300
           CNLLGRGEARSCKKLEKANEKINKLKVRVQELEKAIEDKDNEVLRGLKDANKQG+QGSVC
Sbjct: 241 CNLLGRGEARSCKKLEKANEKINKLKVRVQELEKAIEDKDNEVLRGLKDANKQGIQGSVC 300

Query: 301 CKCNMVSAKNKPAASPDASGSTASQINEPLSSRKGKVLDLASDVRTSDLTLVDTAKDMSF 360
           CKCNMVSAKNKPAASPDASGSTASQINEPLSSRKGKVLDLASDVRTS LTLVDTA+D+  
Sbjct: 301 CKCNMVSAKNKPAASPDASGSTASQINEPLSSRKGKVLDLASDVRTSFLTLVDTAEDIRS 360

Query: 361 SMSKKTSELSHIEHANLNSTIECQISEEGNRMSYHPQLTSCNLVRKKAPERTTNPVNTIP 420
           SM KKT E S +E+A  +STIE QI+EEGNRMSYHPQLTSCNLVRKKAPERTTN VN +P
Sbjct: 361 SMDKKTFEFSQVENAK-DSTIERQINEEGNRMSYHPQLTSCNLVRKKAPERTTNSVNRMP 420

Query: 421 PTATVNNIVRTAVPGDECMPLVVEDTAEPEPVLINIRRESSSHISLNKP-GISFSGGLLG 480
            T TVNNIV TA+PGDECMPL+VEDTAEPEPVL+NIRRESSSHISLNKP GISFSGGLLG
Sbjct: 421 STTTVNNIVGTALPGDECMPLLVEDTAEPEPVLVNIRRESSSHISLNKPGGISFSGGLLG 480

Query: 481 PDGSNRYLGKWCKRGLTNDSSAMQGSRSGELIVVGADGRGGQIKVLRSQNESSLSDTTSA 540
           PDGSNRYLGKWCKRG TN SSAMQGSRSGELIVVGADGRGGQIKVLRSQNESSLSD TSA
Sbjct: 481 PDGSNRYLGKWCKRGQTNGSSAMQGSRSGELIVVGADGRGGQIKVLRSQNESSLSDATSA 540

Query: 541 GAKRFKNGVKNVNMRIENFFSRC 563
           GAKRFKNGVKNVNMRIENFFSRC
Sbjct: 541 GAKRFKNGVKNVNMRIENFFSRC 562

BLAST of HG10010533 vs. NCBI nr
Match: XP_004138487.2 (E3 ubiquitin-protein ligase TRAIP isoform X1 [Cucumis sativus])

HSP 1 Score: 947.6 bits (2448), Expect = 4.9e-272
Identity = 495/563 (87.92%), Postives = 522/563 (92.72%), Query Frame = 0

Query: 1   MDNGCGIAKTICSICYEDLKPIIEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC 60
           MDNGCG AKTICSICYEDLKPIIEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCP+C
Sbjct: 1   MDNGCGFAKTICSICYEDLKPIIEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPVC 60

Query: 61  KQTCVGKDVARLYFQSVGDANDSMLAKKPADWVEKDPELLRSEVKRLEMKVSVLNSALET 120
           KQTCVGKDVARLYFQSVGDANDSMLAKKPADW+EKDPELLRSEVKRLEM+VSVLNS LET
Sbjct: 61  KQTCVGKDVARLYFQSVGDANDSMLAKKPADWIEKDPELLRSEVKRLEMRVSVLNSTLET 120

Query: 121 QGKDLKELHEELQLCQDELKKEVGLRNDALKQKTSMSQLLHLKSEELEKSTVECKRLHER 180
           QGKD+KELHEELQLCQDELKKEVGLRNDA+KQKTSMSQLLHLKSEELE +TVECKRLHER
Sbjct: 121 QGKDIKELHEELQLCQDELKKEVGLRNDAMKQKTSMSQLLHLKSEELENTTVECKRLHER 180

Query: 181 TMSLAKELAAFKLVSDVDLDEEEVLKLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAK 240
           +M+LAKELAAFKL+S+VDLDEEEVLKLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAK
Sbjct: 181 SMALAKELAAFKLISEVDLDEEEVLKLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAK 240

Query: 241 CNLLGRGEARSCKKLEKANEKINKLKVRVQELEKAIEDKDNEVLRGLKDANKQGVQGSVC 300
           CNLLGRGEARSCKKLEKANEKINKLKVRVQELEKAIE+KDNEVLRGLKDA K GVQGSVC
Sbjct: 241 CNLLGRGEARSCKKLEKANEKINKLKVRVQELEKAIEEKDNEVLRGLKDAKKLGVQGSVC 300

Query: 301 CKCNMVSAKNKPAASPDASGSTASQINEPLSSRKGKVLDLASDVRTSDLTLVDTAKDMSF 360
           CKCNMVSAKNK AA+ D   STASQINEPLSSRK K+ D  SD RTS LT VD A++   
Sbjct: 301 CKCNMVSAKNKSAAATDTLDSTASQINEPLSSRKKKIYDFPSDTRTSHLTTVDIAENTCS 360

Query: 361 SMSKKTSELSHIEHANLNSTIECQISEEGNRMSYHPQLTSCNLVRKKAPERTTNPVNTIP 420
           SM+KKTSE S +E+A  +ST+E QISEEGNR+SYHPQLTSCNLVRKKA ERTTN VN +P
Sbjct: 361 SMNKKTSECSQVENAK-DSTMESQISEEGNRVSYHPQLTSCNLVRKKASERTTNSVNPMP 420

Query: 421 PTATVNNIVRTAVPGDECMPLVVEDTAEPEPVLINIRRESSSHISLNK-PGISFSGGLLG 480
            T TV+NIVRTAVPGD CMPL+VED+AEPE VLINIRRESSSHISLNK  G+SFSGGLLG
Sbjct: 421 ST-TVSNIVRTAVPGDACMPLLVEDSAEPEAVLINIRRESSSHISLNKLGGMSFSGGLLG 480

Query: 481 PDGSNRYLGKWCKRGLTNDSSAMQGSRSGELIVVGADGRGGQIKVLRSQNESSLSDTTSA 540
           P GSN YLGKWCKRG TNDSSAMQGSRSGELI VGADGRGGQIKVLRSQNESSLSD TSA
Sbjct: 481 PSGSNMYLGKWCKRGQTNDSSAMQGSRSGELIAVGADGRGGQIKVLRSQNESSLSDATSA 540

Query: 541 GAKRFKNGVKNVNMRIENFFSRC 563
           G KRFKNGVKNVNMRIENFFS+C
Sbjct: 541 GTKRFKNGVKNVNMRIENFFSKC 561

BLAST of HG10010533 vs. NCBI nr
Match: XP_008458166.1 (PREDICTED: E3 ubiquitin-protein ligase TRAIP-like [Cucumis melo])

HSP 1 Score: 930.6 bits (2404), Expect = 6.2e-267
Identity = 489/563 (86.86%), Postives = 515/563 (91.47%), Query Frame = 0

Query: 1   MDNGCGIAKTICSICYEDLKPIIEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC 60
           MDNGCGIAKTICSICYEDLKPIIEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC
Sbjct: 1   MDNGCGIAKTICSICYEDLKPIIEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC 60

Query: 61  KQTCVGKDVARLYFQSVGDANDSMLAKKPADWVEKDPELLRSEVKRLEMKVSVLNSALET 120
           KQTCVGKDVARLYFQSVGDAND MLAKKPADW+EKDPELLRSE+KRLEM+VSVLNS LET
Sbjct: 61  KQTCVGKDVARLYFQSVGDANDPMLAKKPADWIEKDPELLRSEIKRLEMRVSVLNSTLET 120

Query: 121 QGKDLKELHEELQLCQDELKKEVGLRNDALKQKTSMSQLLHLKSEELEKSTVECKRLHER 180
           QGKD+KELHEELQLCQDELKKEVGLRNDA+KQK  MSQLLHLKSEELE STVECKRLHER
Sbjct: 121 QGKDIKELHEELQLCQDELKKEVGLRNDAMKQKILMSQLLHLKSEELENSTVECKRLHER 180

Query: 181 TMSLAKELAAFKLVSDVDLDEEEVLKLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAK 240
           +M+LAKELAAFKL+S+VDLDEEEVLKLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAK
Sbjct: 181 SMALAKELAAFKLISEVDLDEEEVLKLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAK 240

Query: 241 CNLLGRGEARSCKKLEKANEKINKLKVRVQELEKAIEDKDNEVLRGLKDANKQGVQGSVC 300
           CNLLGRGEARSCKKLEKAN KINKLKVRVQELEKAIEDKDNEVLRGLKDA K GVQGSVC
Sbjct: 241 CNLLGRGEARSCKKLEKANGKINKLKVRVQELEKAIEDKDNEVLRGLKDAKKLGVQGSVC 300

Query: 301 CKCNMVSAKNKPAASPDASGSTASQINEPLSSRKGKVLDLASDVRTSDLTLVDTAKDMSF 360
           CKCNMVSAKNK AA+ D   STASQINEPLSSRK K+ D  S   TS LT VD A+++  
Sbjct: 301 CKCNMVSAKNKSAAAADTLPSTASQINEPLSSRKEKIYDFPSGTHTSYLTTVDIAENICS 360

Query: 361 SMSKKTSELSHIEHANLNSTIECQISEEGNRMSYHPQLTSCNLVRKKAPERTTNPVNTIP 420
           S++KKTSE S +E+A  +ST+E QISEEGNRMSYHPQLTSCNLVRKKA E+TTN VN +P
Sbjct: 361 SVNKKTSECSQVENAK-DSTMESQISEEGNRMSYHPQLTSCNLVRKKASEKTTNSVNPMP 420

Query: 421 PTATVNNIVRTAVPGDECMPLVVEDTAEPEPVLINIRRESSSHISLNKP-GISFSGGLLG 480
            T T    VRTAVPGDE MPL+VED+AEPE VLINIRRESSSHISLNKP GISFSGGLLG
Sbjct: 421 STTT----VRTAVPGDEGMPLLVEDSAEPEAVLINIRRESSSHISLNKPGGISFSGGLLG 480

Query: 481 PDGSNRYLGKWCKRGLTNDSSAMQGSRSGELIVVGADGRGGQIKVLRSQNESSLSDTTSA 540
           P+GSN YLGKWCKRG TN SSAMQGSRSGELI VGADGRGGQIKVLRSQNESSLSD TSA
Sbjct: 481 PNGSNMYLGKWCKRGQTNGSSAMQGSRSGELIAVGADGRGGQIKVLRSQNESSLSDATSA 540

Query: 541 GAKRFKNGVKNVNMRIENFFSRC 563
           G KRFKNGVKNVNMRIENFFS+C
Sbjct: 541 GTKRFKNGVKNVNMRIENFFSKC 558

BLAST of HG10010533 vs. NCBI nr
Match: XP_023547669.1 (uncharacterized protein LOC111806541 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 915.6 bits (2365), Expect = 2.1e-262
Identity = 480/562 (85.41%), Postives = 515/562 (91.64%), Query Frame = 0

Query: 1   MDNGCGIAKTICSICYEDLKPIIEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC 60
           MDNGCGI+KTICSICYEDLKPI+EDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC
Sbjct: 1   MDNGCGISKTICSICYEDLKPIVEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC 60

Query: 61  KQTCVGKDVARLYFQSVGDANDSMLAKKPADWVEKDPELLRSEVKRLEMKVSVLNSALET 120
           KQ CV KDVARLYFQSVGDANDSMLAKKPADWVEKDPELLRSEV+RLEMKV+VLNS +ET
Sbjct: 61  KQNCVAKDVARLYFQSVGDANDSMLAKKPADWVEKDPELLRSEVRRLEMKVTVLNSTMET 120

Query: 121 QGKDLKELHEELQLCQDELKKEVGLRNDALKQKTSMSQLLHLKSEELEKSTVECKRLHER 180
           QGKDLKEL EELQ CQDE+KKEVGLRN+A+KQKTSMSQLL+LKSEELEKSTVECKRL+ER
Sbjct: 121 QGKDLKELIEELQFCQDEVKKEVGLRNEAMKQKTSMSQLLNLKSEELEKSTVECKRLNER 180

Query: 181 TMSLAKELAAFKLVSDVDLDEEEVLKLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAK 240
           +M+LAKELAAFKLVS+VDLDEEEV KLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAK
Sbjct: 181 SMALAKELAAFKLVSEVDLDEEEVRKLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAK 240

Query: 241 CNLLGRGEARSCKKLEKANEKINKLKVRVQELEKAIEDKDNEVLRGLKDANKQGVQGSVC 300
           CNLLGRGEAR CKKLEKA EKINKLKVRVQELEK+IEDKDNE LRGLK+A K GVQGSVC
Sbjct: 241 CNLLGRGEARYCKKLEKAKEKINKLKVRVQELEKSIEDKDNEALRGLKEAKKSGVQGSVC 300

Query: 301 CKCNMVSAKNKPAASPDASGSTASQINEPLSSRKGKVLDLASDVRTSDLTLVDTAKDMSF 360
           CKCN V+AKNK AASPDASGSTASQ+NEPLSSRKGK  D ASDV  +DLTLVD+AK M  
Sbjct: 301 CKCNTVAAKNKLAASPDASGSTASQMNEPLSSRKGKNCDFASDVHITDLTLVDSAKSMCS 360

Query: 361 SMSKKTSELSHIEHANLNSTIECQIS-EEGNRMSYHPQLTSCNLVRKKAPERTTNPVNTI 420
           SM+KK+S+ S I+ ANL+S IE Q+S EE ++ SYHPQLT CNLVRKKA ERTTN VNTI
Sbjct: 361 SMNKKSSDFSQIKEANLDSNIERQVSEEEADQRSYHPQLTGCNLVRKKAAERTTNLVNTI 420

Query: 421 PPTATVNNIVRTAVPGDECMPLVVEDTAEPEPVLINIRRESSSHISLNKPGISFSGGLLG 480
           PPTATVNN   TAVPGDECMPLV+EDTAE EPVL NIRRESSSH+S NKPGISFSGG+LG
Sbjct: 421 PPTATVNNTSTTAVPGDECMPLVIEDTAEFEPVL-NIRRESSSHLSHNKPGISFSGGILG 480

Query: 481 PDGSNRYLGKWCKRGLTNDSSAMQGSRSGELIVVGADGRGGQIKVLRSQNESSLSDTTSA 540
           PDGSNR+LGKWCKRGLTN   AM GSRSG+LI VGADGRGGQIKVLRSQ+ESS  DT SA
Sbjct: 481 PDGSNRFLGKWCKRGLTN-GGAMPGSRSGDLIAVGADGRGGQIKVLRSQHESSRIDTVSA 540

Query: 541 GAKRFKNGVKNVNMRIENFFSR 562
           GAKR KNGVK+VNMRIE+FFSR
Sbjct: 541 GAKRHKNGVKDVNMRIEHFFSR 560

BLAST of HG10010533 vs. NCBI nr
Match: XP_022958831.1 (uncharacterized protein LOC111459984 isoform X1 [Cucurbita moschata])

HSP 1 Score: 910.6 bits (2352), Expect = 6.7e-261
Identity = 478/562 (85.05%), Postives = 514/562 (91.46%), Query Frame = 0

Query: 1   MDNGCGIAKTICSICYEDLKPIIEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC 60
           MDNGCGI+KTICSICYEDLKPI+EDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC
Sbjct: 1   MDNGCGISKTICSICYEDLKPIVEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC 60

Query: 61  KQTCVGKDVARLYFQSVGDANDSMLAKKPADWVEKDPELLRSEVKRLEMKVSVLNSALET 120
           KQ CV KDVARLYFQSVGDANDSMLAKKPADWVEKDPELLRSEV+RLEMKV+VLNS LET
Sbjct: 61  KQNCVAKDVARLYFQSVGDANDSMLAKKPADWVEKDPELLRSEVRRLEMKVTVLNSTLET 120

Query: 121 QGKDLKELHEELQLCQDELKKEVGLRNDALKQKTSMSQLLHLKSEELEKSTVECKRLHER 180
           QGKDLKEL EELQ CQDE+KKEVGLRN+A+KQKTSMSQLL+LKSEELEKSTVEC+RLH+R
Sbjct: 121 QGKDLKELIEELQFCQDEVKKEVGLRNEAMKQKTSMSQLLNLKSEELEKSTVECERLHDR 180

Query: 181 TMSLAKELAAFKLVSDVDLDEEEVLKLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAK 240
           +M+LAKELAAFKLVS+VDLDEEEV KLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAK
Sbjct: 181 SMALAKELAAFKLVSEVDLDEEEVRKLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAK 240

Query: 241 CNLLGRGEARSCKKLEKANEKINKLKVRVQELEKAIEDKDNEVLRGLKDANKQGVQGSVC 300
           CNLLGRGEAR CKKLEKA EKINKLKVRVQELEK+IEDKDNE LRGLK+A K GVQGSVC
Sbjct: 241 CNLLGRGEARYCKKLEKAKEKINKLKVRVQELEKSIEDKDNEALRGLKEAKKSGVQGSVC 300

Query: 301 CKCNMVSAKNKPAASPDASGSTASQINEPLSSRKGKVLDLASDVRTSDLTLVDTAKDMSF 360
           CKCN V+AKNK AASPDASGS ASQ+NEPLSSRKGK  D ASDV  +DLTLVD+AK+M  
Sbjct: 301 CKCNTVTAKNKLAASPDASGSAASQMNEPLSSRKGKNCDFASDVLITDLTLVDSAKNMFS 360

Query: 361 SMSKKTSELSHIEHANLNSTIECQIS-EEGNRMSYHPQLTSCNLVRKKAPERTTNPVNTI 420
           SM+KK+S+ S I+ ANL+S IE QIS EE ++ SYHPQLT CNLVRKKA ERTTN VNTI
Sbjct: 361 SMNKKSSDFSQIKEANLDSNIERQISEEEADQRSYHPQLTGCNLVRKKAAERTTNLVNTI 420

Query: 421 PPTATVNNIVRTAVPGDECMPLVVEDTAEPEPVLINIRRESSSHISLNKPGISFSGGLLG 480
           PPTATVNN   TAVPGDECMPLV+ED+AE EPVL NIRRESSSH+S NKPGISFSGG+LG
Sbjct: 421 PPTATVNNTSTTAVPGDECMPLVIEDSAEFEPVL-NIRRESSSHLSHNKPGISFSGGILG 480

Query: 481 PDGSNRYLGKWCKRGLTNDSSAMQGSRSGELIVVGADGRGGQIKVLRSQNESSLSDTTSA 540
           PDGSNR+LGKWCKRGLTN   AM GSRSG+LI VGADGRGGQIKVLRSQ+ESS  D  SA
Sbjct: 481 PDGSNRFLGKWCKRGLTN-GGAMPGSRSGDLIAVGADGRGGQIKVLRSQHESSRFDAVSA 540

Query: 541 GAKRFKNGVKNVNMRIENFFSR 562
           GAKR KNGVK+VNMRIE+FFSR
Sbjct: 541 GAKRHKNGVKDVNMRIEHFFSR 560

BLAST of HG10010533 vs. ExPASy Swiss-Prot
Match: Q8VIG6 (E3 ubiquitin-protein ligase TRAIP OS=Mus musculus OX=10090 GN=Traip PE=1 SV=2)

HSP 1 Score: 47.4 bits (111), Expect = 6.3e-04
Identity = 58/278 (20.86%), Postives = 127/278 (45.68%), Query Frame = 0

Query: 9   KTICSICYEDLKPIIEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMCKQTCVGKD 68
           + +C+IC  D      D+ +I  CGH FH  CL QWFE   +     +CP C+     K 
Sbjct: 4   RALCTIC-SDFFDHSRDVAAIH-CGHTFHLQCLIQWFETAPS----RTCPQCRIQVGKKT 63

Query: 69  VARLYFQSVGDANDSMLAKKPADWVEKDPELLRSEVKRLEMKVSVLNSALETQGKDLKEL 128
           +    F  +    +++L    A++++ + + +++++ + + +     + ++T    L+E 
Sbjct: 64  IINKLFFDLAQEEENVL---DAEFLKNELDSVKAQLSQKDREKRDSQAIIDTLRDTLEER 123

Query: 129 HEELQLCQDELKKEVGLRNDALKQKTSMSQLLHLKSEELEKSTVECKRLHERTMSLAK-E 188
           +  ++  Q+ L K   L +   KQ   + Q    + +E +++  E  RL  +  ++ + E
Sbjct: 124 NATVESLQNALNKAEMLCSTLKKQMKFLEQ----RQDETKQAREEAHRLKCKMKTMEQIE 183

Query: 189 LAAFKLVSDVDLDEEEVLKLSSLGSGADNKDTIDI--LRKSLVLRNRSYKELMAKCNLLG 248
           L      S+V    EE+++   +G  A  +  +    L+K       + K      + L 
Sbjct: 184 LLLQSQRSEV----EEMIRDMGVGQSAVEQLAVYCVSLKKEYENLKEARKATGELADRLK 243

Query: 249 RGEARSCKKLEKANEKINKLKVRVQELEKAIEDKDNEV 284
           +    S  KL+  N ++++ K+ ++  +K ++  D E+
Sbjct: 244 KDLVSSRSKLKTLNTELDQAKLELRSAQKDLQSADQEI 264

BLAST of HG10010533 vs. ExPASy TrEMBL
Match: A0A1S3C7C7 (E3 ubiquitin-protein ligase TRAIP-like OS=Cucumis melo OX=3656 GN=LOC103497686 PE=4 SV=1)

HSP 1 Score: 930.6 bits (2404), Expect = 3.0e-267
Identity = 489/563 (86.86%), Postives = 515/563 (91.47%), Query Frame = 0

Query: 1   MDNGCGIAKTICSICYEDLKPIIEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC 60
           MDNGCGIAKTICSICYEDLKPIIEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC
Sbjct: 1   MDNGCGIAKTICSICYEDLKPIIEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC 60

Query: 61  KQTCVGKDVARLYFQSVGDANDSMLAKKPADWVEKDPELLRSEVKRLEMKVSVLNSALET 120
           KQTCVGKDVARLYFQSVGDAND MLAKKPADW+EKDPELLRSE+KRLEM+VSVLNS LET
Sbjct: 61  KQTCVGKDVARLYFQSVGDANDPMLAKKPADWIEKDPELLRSEIKRLEMRVSVLNSTLET 120

Query: 121 QGKDLKELHEELQLCQDELKKEVGLRNDALKQKTSMSQLLHLKSEELEKSTVECKRLHER 180
           QGKD+KELHEELQLCQDELKKEVGLRNDA+KQK  MSQLLHLKSEELE STVECKRLHER
Sbjct: 121 QGKDIKELHEELQLCQDELKKEVGLRNDAMKQKILMSQLLHLKSEELENSTVECKRLHER 180

Query: 181 TMSLAKELAAFKLVSDVDLDEEEVLKLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAK 240
           +M+LAKELAAFKL+S+VDLDEEEVLKLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAK
Sbjct: 181 SMALAKELAAFKLISEVDLDEEEVLKLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAK 240

Query: 241 CNLLGRGEARSCKKLEKANEKINKLKVRVQELEKAIEDKDNEVLRGLKDANKQGVQGSVC 300
           CNLLGRGEARSCKKLEKAN KINKLKVRVQELEKAIEDKDNEVLRGLKDA K GVQGSVC
Sbjct: 241 CNLLGRGEARSCKKLEKANGKINKLKVRVQELEKAIEDKDNEVLRGLKDAKKLGVQGSVC 300

Query: 301 CKCNMVSAKNKPAASPDASGSTASQINEPLSSRKGKVLDLASDVRTSDLTLVDTAKDMSF 360
           CKCNMVSAKNK AA+ D   STASQINEPLSSRK K+ D  S   TS LT VD A+++  
Sbjct: 301 CKCNMVSAKNKSAAAADTLPSTASQINEPLSSRKEKIYDFPSGTHTSYLTTVDIAENICS 360

Query: 361 SMSKKTSELSHIEHANLNSTIECQISEEGNRMSYHPQLTSCNLVRKKAPERTTNPVNTIP 420
           S++KKTSE S +E+A  +ST+E QISEEGNRMSYHPQLTSCNLVRKKA E+TTN VN +P
Sbjct: 361 SVNKKTSECSQVENAK-DSTMESQISEEGNRMSYHPQLTSCNLVRKKASEKTTNSVNPMP 420

Query: 421 PTATVNNIVRTAVPGDECMPLVVEDTAEPEPVLINIRRESSSHISLNKP-GISFSGGLLG 480
            T T    VRTAVPGDE MPL+VED+AEPE VLINIRRESSSHISLNKP GISFSGGLLG
Sbjct: 421 STTT----VRTAVPGDEGMPLLVEDSAEPEAVLINIRRESSSHISLNKPGGISFSGGLLG 480

Query: 481 PDGSNRYLGKWCKRGLTNDSSAMQGSRSGELIVVGADGRGGQIKVLRSQNESSLSDTTSA 540
           P+GSN YLGKWCKRG TN SSAMQGSRSGELI VGADGRGGQIKVLRSQNESSLSD TSA
Sbjct: 481 PNGSNMYLGKWCKRGQTNGSSAMQGSRSGELIAVGADGRGGQIKVLRSQNESSLSDATSA 540

Query: 541 GAKRFKNGVKNVNMRIENFFSRC 563
           G KRFKNGVKNVNMRIENFFS+C
Sbjct: 541 GTKRFKNGVKNVNMRIENFFSKC 558

BLAST of HG10010533 vs. ExPASy TrEMBL
Match: A0A6J1H385 (uncharacterized protein LOC111459984 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459984 PE=4 SV=1)

HSP 1 Score: 910.6 bits (2352), Expect = 3.2e-261
Identity = 478/562 (85.05%), Postives = 514/562 (91.46%), Query Frame = 0

Query: 1   MDNGCGIAKTICSICYEDLKPIIEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC 60
           MDNGCGI+KTICSICYEDLKPI+EDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC
Sbjct: 1   MDNGCGISKTICSICYEDLKPIVEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC 60

Query: 61  KQTCVGKDVARLYFQSVGDANDSMLAKKPADWVEKDPELLRSEVKRLEMKVSVLNSALET 120
           KQ CV KDVARLYFQSVGDANDSMLAKKPADWVEKDPELLRSEV+RLEMKV+VLNS LET
Sbjct: 61  KQNCVAKDVARLYFQSVGDANDSMLAKKPADWVEKDPELLRSEVRRLEMKVTVLNSTLET 120

Query: 121 QGKDLKELHEELQLCQDELKKEVGLRNDALKQKTSMSQLLHLKSEELEKSTVECKRLHER 180
           QGKDLKEL EELQ CQDE+KKEVGLRN+A+KQKTSMSQLL+LKSEELEKSTVEC+RLH+R
Sbjct: 121 QGKDLKELIEELQFCQDEVKKEVGLRNEAMKQKTSMSQLLNLKSEELEKSTVECERLHDR 180

Query: 181 TMSLAKELAAFKLVSDVDLDEEEVLKLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAK 240
           +M+LAKELAAFKLVS+VDLDEEEV KLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAK
Sbjct: 181 SMALAKELAAFKLVSEVDLDEEEVRKLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAK 240

Query: 241 CNLLGRGEARSCKKLEKANEKINKLKVRVQELEKAIEDKDNEVLRGLKDANKQGVQGSVC 300
           CNLLGRGEAR CKKLEKA EKINKLKVRVQELEK+IEDKDNE LRGLK+A K GVQGSVC
Sbjct: 241 CNLLGRGEARYCKKLEKAKEKINKLKVRVQELEKSIEDKDNEALRGLKEAKKSGVQGSVC 300

Query: 301 CKCNMVSAKNKPAASPDASGSTASQINEPLSSRKGKVLDLASDVRTSDLTLVDTAKDMSF 360
           CKCN V+AKNK AASPDASGS ASQ+NEPLSSRKGK  D ASDV  +DLTLVD+AK+M  
Sbjct: 301 CKCNTVTAKNKLAASPDASGSAASQMNEPLSSRKGKNCDFASDVLITDLTLVDSAKNMFS 360

Query: 361 SMSKKTSELSHIEHANLNSTIECQIS-EEGNRMSYHPQLTSCNLVRKKAPERTTNPVNTI 420
           SM+KK+S+ S I+ ANL+S IE QIS EE ++ SYHPQLT CNLVRKKA ERTTN VNTI
Sbjct: 361 SMNKKSSDFSQIKEANLDSNIERQISEEEADQRSYHPQLTGCNLVRKKAAERTTNLVNTI 420

Query: 421 PPTATVNNIVRTAVPGDECMPLVVEDTAEPEPVLINIRRESSSHISLNKPGISFSGGLLG 480
           PPTATVNN   TAVPGDECMPLV+ED+AE EPVL NIRRESSSH+S NKPGISFSGG+LG
Sbjct: 421 PPTATVNNTSTTAVPGDECMPLVIEDSAEFEPVL-NIRRESSSHLSHNKPGISFSGGILG 480

Query: 481 PDGSNRYLGKWCKRGLTNDSSAMQGSRSGELIVVGADGRGGQIKVLRSQNESSLSDTTSA 540
           PDGSNR+LGKWCKRGLTN   AM GSRSG+LI VGADGRGGQIKVLRSQ+ESS  D  SA
Sbjct: 481 PDGSNRFLGKWCKRGLTN-GGAMPGSRSGDLIAVGADGRGGQIKVLRSQHESSRFDAVSA 540

Query: 541 GAKRFKNGVKNVNMRIENFFSR 562
           GAKR KNGVK+VNMRIE+FFSR
Sbjct: 541 GAKRHKNGVKDVNMRIEHFFSR 560

BLAST of HG10010533 vs. ExPASy TrEMBL
Match: A0A6J1L583 (uncharacterized protein LOC111499239 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499239 PE=4 SV=1)

HSP 1 Score: 904.0 bits (2335), Expect = 3.0e-259
Identity = 476/562 (84.70%), Postives = 512/562 (91.10%), Query Frame = 0

Query: 1   MDNGCGIAKTICSICYEDLKPIIEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC 60
           MDNGCGI+KTICSICYEDLKPI+EDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC
Sbjct: 1   MDNGCGISKTICSICYEDLKPIVEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC 60

Query: 61  KQTCVGKDVARLYFQSVGDANDSMLAKKPADWVEKDPELLRSEVKRLEMKVSVLNSALET 120
           KQ CV KDVARLYFQSVGDANDSMLAKKPADWVEKDPELLRSEV+RLEMKV+VLNS LE+
Sbjct: 61  KQNCVAKDVARLYFQSVGDANDSMLAKKPADWVEKDPELLRSEVRRLEMKVTVLNSTLES 120

Query: 121 QGKDLKELHEELQLCQDELKKEVGLRNDALKQKTSMSQLLHLKSEELEKSTVECKRLHER 180
           QGKDLKEL EELQ CQDE+KKEVGLRN+A+KQKTSMSQLL+LKSEELEKSTVECKRLHER
Sbjct: 121 QGKDLKELIEELQFCQDEVKKEVGLRNEAMKQKTSMSQLLNLKSEELEKSTVECKRLHER 180

Query: 181 TMSLAKELAAFKLVSDVDLDEEEVLKLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAK 240
           +M+LAKELAAFKLVS+VDLDEEEV KLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAK
Sbjct: 181 SMALAKELAAFKLVSEVDLDEEEVRKLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAK 240

Query: 241 CNLLGRGEARSCKKLEKANEKINKLKVRVQELEKAIEDKDNEVLRGLKDANKQGVQGSVC 300
           CNLLGRGEAR CKKLEKA EKINKL VRVQELEK+IEDKDNE LRGLK+A K GVQGSVC
Sbjct: 241 CNLLGRGEARYCKKLEKAKEKINKLMVRVQELEKSIEDKDNEALRGLKEAKKSGVQGSVC 300

Query: 301 CKCNMVSAKNKPAASPDASGSTASQINEPLSSRKGKVLDLASDVRTSDLTLVDTAKDMSF 360
           CKCN V+AKNK AASPDASGSTASQ+NEPLSSRKGK  D ASDV  +DLTLVD+AK+M  
Sbjct: 301 CKCNTVAAKNKLAASPDASGSTASQMNEPLSSRKGKNCDFASDVLITDLTLVDSAKNMCS 360

Query: 361 SMSKKTSELSHIEHANLNSTIECQIS-EEGNRMSYHPQLTSCNLVRKKAPERTTNPVNTI 420
           SM+KK+S+ S I+ ANL+S IE Q+S EE ++ SYHPQLT CNLVRKKA ERTTN VNTI
Sbjct: 361 SMNKKSSDFSQIKEANLDSNIERQVSEEEADQRSYHPQLTGCNLVRKKAVERTTNLVNTI 420

Query: 421 PPTATVNNIVRTAVPGDECMPLVVEDTAEPEPVLINIRRESSSHISLNKPGISFSGGLLG 480
           P TATVNN   TAVPGDECMPLV+ +TAE EPVL NIRRESSSH+S NKPGISFSGG+LG
Sbjct: 421 PATATVNNTSTTAVPGDECMPLVIVETAEFEPVL-NIRRESSSHLSHNKPGISFSGGILG 480

Query: 481 PDGSNRYLGKWCKRGLTNDSSAMQGSRSGELIVVGADGRGGQIKVLRSQNESSLSDTTSA 540
           PDGSNR+LGKWCKRGLTN   AM GSRSG+LI VGADGRGGQIKVLRSQ+ESS  D  SA
Sbjct: 481 PDGSNRFLGKWCKRGLTN-GGAMPGSRSGDLIAVGADGRGGQIKVLRSQHESSRIDAVSA 540

Query: 541 GAKRFKNGVKNVNMRIENFFSR 562
           GAKR KNGVK+VNMRIE+FFSR
Sbjct: 541 GAKRPKNGVKDVNMRIEHFFSR 560

BLAST of HG10010533 vs. ExPASy TrEMBL
Match: A0A6J1D0D3 (uncharacterized protein LOC111016223 OS=Momordica charantia OX=3673 GN=LOC111016223 PE=4 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 4.7e-244
Identity = 455/569 (79.96%), Postives = 503/569 (88.40%), Query Frame = 0

Query: 1   MDNGCGIAKTICSICYEDLKPIIEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC 60
           MDNGCGIAKTICSICYEDLKPI+EDLQSI+ICGHVFHELCLQQWFEYCS+GKKKYSCPMC
Sbjct: 1   MDNGCGIAKTICSICYEDLKPIVEDLQSIAICGHVFHELCLQQWFEYCSSGKKKYSCPMC 60

Query: 61  KQTCVGKDVARLYFQSVGDA-NDSMLAKKPADWVEKDPELLRSEVKRLEMKVSVLNSALE 120
           KQTC  KDVARLYFQSVGDA NDS+L +KP DWVE+DPE LR EVKRLEMKVSVLNSALE
Sbjct: 61  KQTCGAKDVARLYFQSVGDASNDSVLTQKPRDWVERDPEALRIEVKRLEMKVSVLNSALE 120

Query: 121 TQGKDLKELHEELQLCQDELKKEVGLRNDALKQKTSMSQLLHLKSEELEKSTVECKRLHE 180
            QGKDLK+L EEL +CQDE+KKEVGLRN+A+KQK  MSQ+LHLKSEEL+KSTVECKRLHE
Sbjct: 121 GQGKDLKQLSEELHICQDEVKKEVGLRNEAVKQKALMSQMLHLKSEELDKSTVECKRLHE 180

Query: 181 RTMSLAKELAAFKLVSDVDLDEEEVLKLSSLGSGADNKDTIDILRKSLVLRNRSYKELMA 240
           R+M+LAKELAAFKLV+++DLDEEEVLKLSSLGSGADNKDTIDILRKSLV+RNRSYKELM 
Sbjct: 181 RSMALAKELAAFKLVTELDLDEEEVLKLSSLGSGADNKDTIDILRKSLVMRNRSYKELMT 240

Query: 241 KCNLLGRGEARSCKKLEKANEKINKLKVRVQELEKAIEDKDNEVLRGLKD---ANKQGVQ 300
           KCNLLGRGEA SCKKL KA EKI KLK RVQELEKA EDK+NEVLRGLKD   A+ QGVQ
Sbjct: 241 KCNLLGRGEAHSCKKLGKAKEKIIKLKARVQELEKAGEDKENEVLRGLKDSKNASIQGVQ 300

Query: 301 GSVCCKCNMVSAKNKPAASPDASGSTASQINEPLSSRKGKVLDLASDVRTSDLTLVDTAK 360
             VCCKCNM+SAKN+PAA PDASGSTASQIN+ LSSRKGK+L+    V TS+L LVDT K
Sbjct: 301 DFVCCKCNMISAKNEPAAPPDASGSTASQINDLLSSRKGKILEF---VGTSNLNLVDTTK 360

Query: 361 DMSFSMSKKTSELSHIEHANLNSTIECQIS-EEGNRMSYHPQLTSCNLVRKKAPERTTNP 420
           ++S SM KKTSE S IE+AN+N  +  QI+ EE ++ SYHPQL +C L+RK+A ERTTNP
Sbjct: 361 NISSSMDKKTSEFSQIENANVNPNLGSQINEEETDQRSYHPQLKTCILIRKRAAERTTNP 420

Query: 421 VNTIPPTATVNNIVRT-AVPGDECMPLVVEDTAEPEPVLINIRRESSSHISLNKPG-ISF 480
           VN +P TATVNN   T AVPGD+CMPLV+EDTA+ EPVL NIRRESSSH+ LNKPG I F
Sbjct: 421 VNVMPSTATVNNTSMTSAVPGDKCMPLVIEDTAQCEPVL-NIRRESSSHLPLNKPGDICF 480

Query: 481 SGGLLGPDGSNRYLGKWCKRGLTNDSSAMQGSRSGELIVVGADGRGGQIKVLRSQNESSL 540
           SGGLLGPDGS+RYLGKWCKRG  N S A+QGSR+GELIVVGADGRGGQIKVLRSQNESSL
Sbjct: 481 SGGLLGPDGSSRYLGKWCKRGQINGSDAIQGSRTGELIVVGADGRGGQIKVLRSQNESSL 540

Query: 541 SDTTSAGAKRFKNGVKNVNMRIENFFSRC 563
            +TT AGAKR KNGVKNVNMRIE+FFSRC
Sbjct: 541 INTTLAGAKRCKNGVKNVNMRIEHFFSRC 565

BLAST of HG10010533 vs. ExPASy TrEMBL
Match: A0A5D3CRM0 (E3 ubiquitin-protein ligase TRAIP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold268G00290 PE=4 SV=1)

HSP 1 Score: 850.1 bits (2195), Expect = 5.2e-243
Identity = 464/605 (76.69%), Postives = 499/605 (82.48%), Query Frame = 0

Query: 1   MDNGCGIAKTICSICYEDLKPIIEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC 60
           MDNGCGIAKTICSICYEDLKPIIEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC
Sbjct: 1   MDNGCGIAKTICSICYEDLKPIIEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMC 60

Query: 61  KQTCVGKDVARLYFQSVGDANDSMLAKKPADWVEKDPELLRSEVKRLEMKVSVLNSALET 120
           KQTCVGKDVARLYFQSVGDAND MLAKKPADW+EKDPELLRSE+KRLEM+VSVLNS LET
Sbjct: 61  KQTCVGKDVARLYFQSVGDANDPMLAKKPADWIEKDPELLRSEIKRLEMRVSVLNSTLET 120

Query: 121 QGKDLKELHEE--------------------------LQLCQDELKKEVGLRNDALKQKT 180
           QGKD+KELHEE                          + LC   +   +GL     +  +
Sbjct: 121 QGKDIKELHEESLKRFSLHFVFSIESFHTVVEFGLIVIWLCLVLVVLSLGLYKWFWEDIS 180

Query: 181 SMSQLLHLK----------------SEELEKSTVECKRLHERTMSLAKELAAFKLVSDVD 240
           S+S+ +  +                 +ELE STVECKRLHER+M+LAKELAAFKL+S+VD
Sbjct: 181 SISKSIDNERSNPSYEIVRKAMLGLCKELENSTVECKRLHERSMALAKELAAFKLISEVD 240

Query: 241 LDEEEVLKLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAKCNLLGRGEARSCKKLEKA 300
           LDEEEVLKLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAKCNLLGRGEARSCKKLEKA
Sbjct: 241 LDEEEVLKLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAKCNLLGRGEARSCKKLEKA 300

Query: 301 NEKINKLKVRVQELEKAIEDKDNEVLRGLKDANKQGVQGSVCCKCNMVSAKNKPAASPDA 360
           N KINKLKVRVQELEKAIEDKDNEVLRGLKDA K GVQGSVCCKCNMVSAKNK AA+ D 
Sbjct: 301 NGKINKLKVRVQELEKAIEDKDNEVLRGLKDAKKLGVQGSVCCKCNMVSAKNKSAAAADT 360

Query: 361 SGSTASQINEPLSSRKGKVLDLASDVRTSDLTLVDTAKDMSFSMSKKTSELSHIEHANLN 420
             STASQINEPLSSRK K+ D  S   TS LT VD A+++  S++KKTSE S +E+A  +
Sbjct: 361 LPSTASQINEPLSSRKEKIYDFPSGTHTSYLTTVDIAENICSSVNKKTSECSQVENAK-D 420

Query: 421 STIECQISEEGNRMSYHPQLTSCNLVRKKAPERTTNPVNTIPPTATVNNIVRTAVPGDEC 480
           ST+E QISEEGNRMSYHPQLTSCNLVRKKA E+TTN VN +P T T    VRTAVPGDE 
Sbjct: 421 STMESQISEEGNRMSYHPQLTSCNLVRKKASEKTTNSVNPMPSTTT----VRTAVPGDEG 480

Query: 481 MPLVVEDTAEPEPVLINIRRESSSHISLNKP-GISFSGGLLGPDGSNRYLGKWCKRGLTN 540
           MPL+VED+AEPE VLINIRRESSSHISLNKP GISFSGGLLGP+GSN YLGKWCKRG TN
Sbjct: 481 MPLLVEDSAEPEAVLINIRRESSSHISLNKPGGISFSGGLLGPNGSNMYLGKWCKRGQTN 540

Query: 541 DSSAMQGSRSGELIVVGADGRGGQIKVLRSQNESSLSDTTSAGAKRFKNGVKNVNMRIEN 563
            SSAMQGSRSGELI VGADGRGGQIKVLRSQNESSLSD TSAG KRFKNGVKNVNMRIEN
Sbjct: 541 GSSAMQGSRSGELIAVGADGRGGQIKVLRSQNESSLSDATSAGTKRFKNGVKNVNMRIEN 600

BLAST of HG10010533 vs. TAIR 10
Match: AT4G01270.1 (RING/U-box superfamily protein )

HSP 1 Score: 332.8 bits (852), Expect = 5.3e-91
Identity = 236/571 (41.33%), Postives = 330/571 (57.79%), Query Frame = 0

Query: 2   DNGCGIAKTICSICYEDLKPIIEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMCK 61
           +N  G A  ICSICYEDLKP++E+LQSIS CGHVFHELCLQQWFEYC +  K+ +CP+CK
Sbjct: 4   ENAAGNA--ICSICYEDLKPVVENLQSISACGHVFHELCLQQWFEYCPSTNKR-NCPICK 63

Query: 62  QTCVGKDVARLYFQSVGDANDSMLAKKPADWVEKDPELLRSEVKRLEMKVSVLNSALETQ 121
           Q C  KD  RLYFQS G+  DS+ + K    +E+DP LLR EVKRLE KV  L SALE +
Sbjct: 64  QKCSLKDPCRLYFQSSGNQTDSIASDKVVG-IEEDPVLLRGEVKRLEGKVQNLTSALEAK 123

Query: 122 GKDLKELHEELQLCQDELKKEVGLRNDALKQKTSMSQLLHLKSEELEKSTVECKRLHERT 181
            K+  E+ ++L  C ++LK++   R +AL++ ++   LL LKSEE  +   +C +L ERT
Sbjct: 124 KKENVEVSDKLHQCNEQLKEDKVKRWEALQEISTTQHLLKLKSEECIQLNSQCVKLQERT 183

Query: 182 MSLAKELAAFKLVSDVDLDEEEVLKLSSLGSGADNKDTIDILRKSLVLRNRSYKELMAKC 241
           ++LAKELA+ KLVSD+ L+E++VLKL+ LG+ A  KDTID L KSLV+RNRSYKEL+AKC
Sbjct: 184 VALAKELASLKLVSDLSLEEDDVLKLALLGNNAKTKDTIDTLVKSLVIRNRSYKELLAKC 243

Query: 242 NLLGRGEARSCKKLEKANEKINKLKVRVQELEKAIEDKDNEVLRGLKDANKQGVQGSVCC 301
           N LGRGEARS +KLEKA EKI KLK R++ELE   E+++N  LR +          +V  
Sbjct: 244 NQLGRGEARSSEKLEKALEKIEKLKKRMRELELITEERENRALRDI----------NVSK 303

Query: 302 KCNMVSAKNKPAASPDASGSTASQIN--EPLSSRKGKVLDLASDVRTSDLTLVDTAKDMS 361
           KC+     ++PA    +S    S  N  E +S+  GK+ +   D  T   + +   +D  
Sbjct: 304 KCSYTEV-SEPAIESMSSFRMLSSDNKVEKISTPPGKLEE--KDGFTIQGSCLRGRED-- 363

Query: 362 FSMSKKTSELSHIEHANLNSTIECQISEEGNRMSYHPQLTSCNLVRKKAPERTTNPVNTI 421
            S   +T  +  ++                    Y P+  +  +       R  N     
Sbjct: 364 -SFVSRTDSVIDVDD------------------DYVPETNTSGI-------RDWN----- 423

Query: 422 PPTATVNNIVRTAVPGDECMPLVVEDTAEPEPVLINIRRESSSHISLNKPGISFSGGLLG 481
                  NI      GD  M   V+D      +  NIR++ +S +S   P  + SG +  
Sbjct: 424 ------TNIEE---KGDNSM---VKD------IKFNIRKDPTSSVS---PYSNGSGNIWQ 483

Query: 482 PDGSNRYLGKWCKRGLTNDSSAMQGS---RSGELIVVGADGRGGQIKVLRSQNESSLSDT 541
             G+NR LG+W K G  N+++   G    R  +LI +G DG+GG+IKVLRS+ + S ++ 
Sbjct: 484 SSGTNRNLGRWSKHGERNEATPSLGGSVPRKDDLISIGPDGKGGRIKVLRSKPQISKTNA 503

Query: 542 TSAGAKRFKNGVKNVN------MRIENFFSR 562
           +S   KRFK G K         ++IE++F +
Sbjct: 544 SSGSGKRFKLGTKTSGSSSQGCLQIEHYFGK 503

BLAST of HG10010533 vs. TAIR 10
Match: AT4G01245.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: RING/U-box superfamily protein (TAIR:AT4G01270.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 60.5 bits (145), Expect = 5.1e-09
Identity = 35/59 (59.32%), Postives = 43/59 (72.88%), Query Frame = 0

Query: 233 SYKELMAKCNLLGRGEARSCKKLEKANEKINKLKVRVQELEKAIEDKDNEVLRGLKDAN 292
           SYKEL+AKCN LGRGEA+S +KLEKA EKI KLK R++ELE   E+      R ++D N
Sbjct: 34  SYKELLAKCNQLGRGEAQSSEKLEKALEKIEKLKKRMRELELITEEN-----RAIRDIN 87

BLAST of HG10010533 vs. TAIR 10
Match: AT1G57730.1 (RING/U-box superfamily protein )

HSP 1 Score: 44.7 bits (104), Expect = 2.9e-04
Identity = 18/57 (31.58%), Postives = 29/57 (50.88%), Query Frame = 0

Query: 12  CSICYEDLKPIIEDLQSISICGHVFHELCLQQWFEYCSNGKKKYSCPMCKQTCVGKD 69
           C+IC ED+   + D Q +  C HVFH  C+ +W  + +       CP+C+     +D
Sbjct: 116 CAICLEDMSQDVHDYQEMPNCPHVFHNDCIYKWLGHSN------LCPLCRTVLEDED 166