HG10010458 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10010458
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptiontRNA pseudouridine synthase
LocationChr06: 22397937 .. 22400581 (+)
RNA-Seq ExpressionHG10010458
SyntenyHG10010458
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGTGCTGGGCGGTGGGTTCGCCTGTTCTTCCTCCCATTTCCCTTCCCCTTTCACCAAGCATTCCATTTCCAAATGGCGCTTCATCTTCCTCCAGTCGTGGATTCCCGGTAACAACCATTCGTCTCTAATCTCTCAGTTCCTCTTTTCATTTTGGCGGTTTAATTGTGACGATGTTGATGAGATCGTTGCCTTGTCTTGATTCTTCAAAAGAATGTGGAGGATTCCTCTTATATCACAGAGCCCCCTTTCCCCAGCTTCAAATGGCGGTTGCTCATCGCTTATGATGGTACCCGATACGCAGGTCCAAGTTTCTTTTTATTATCTATAATTGTCTATTCGGGCGAATGAATTGAACGATTTTGTGTCCATATACCAGCTTTATCTTTTTGGGTTAACAGTAATTCTTACTGGCTTAAGCTTTTGGGGTTAGTCGTTGTTGCCTGTTGGATGTCTTTTTCATCATCAGTATCAATTCAAGAGAGCCCCATAATATCATAAATATAATATTATATCATCTAACCAAGAGAGCATCAAAATTGTGGCAGTGAGCACAAACTGCTCTGAGTTAACTCATAATAATAAGTAATTTTGAGGCTCTCTTGGTGTTAGATGATATAATATTATATTTATCTTTATCCATAAACTTAAGTTTTTGAATCAATGGGTGATTTAAGATGATATTAAAGCAGGTGGTCTAGGATGTCTTGTGTTCAAACTCTTACAATGTTATTTTCTCCTCAATGGGTCTTCTACATATTTCAAACCCACATGTGAGGAGAGTGTTAGATGATATAATATTAAATTTACCTTCACCCATAAACTTGAGCTTTTGGGTCAATCATTACTTTAAGACTTGGTCCAAAGGTTTGATAAAATAATTTTGAATATCTTGTTGTTAAACTAGGCTGGCAATATCAGCGGTCACCACCCACTATTCAGTGCCTCATCGAAGAAGCCTTGATTGGAGCAACAAAGCTTGAAAGGAAGGATCTCCATTTAGTTGGTGCAAGCAGAACAGATAAAGGAGTTCATGCCTGGGGTCAGGTCTGATGTGCTTTTATGGAGTTGGGTTAAAACACGTCATTGGAATACAATGCATGCTGTTGCTTACAACAAGTTGAGGTTTCAGGTGGCTCACTTTATTACGCCATTTAACTACGATAGCTTGGAAAACGTTCATGCTGCACTGAATGGTCTTCTTCCCGTGGATATAAGAGTTCGAGAGATCAGTCCTGCTGCGCCTGACTTTCATGCTCGGTTTTCTACGTTAAGCAAGGTTTATCACTACAAGATATATAATGGCGCAATCTTGGATCCATTTCAGCGTTGCTACACTTACCATTGTGTTTATAAACTCAACTATGCAGCCATGAGAGAAGCTGCAAATTATTTTGTTGGGAAGCATGATTTTTCTGCTTTTACAAATGCAGCACATAATGATCGAGTGGTAAATCCGGTGAAGGTCATTTTTCGTTTTGATGTCATAGAAATGGTAAAGTTTTAAGGTTCTTTAATCACTGATTTGTTTGTTTAAATTACAAATTTAGTCTATGAACTTTGAGATTTATGTCTATTTGGTCATCTTTCAAAAGTGTTTAATAGGTTGTTGAACCTTCAACCTTGTGCGTTATAGGACCTTGAACTTTAAAAAATATTGAAATGTTCTTAAACTTTCAATTTCATGTTCGTTAGATCACTTAATTTTCAATTGTGTTTGTACTTGACCTATTCGATACCTTTTAAAATTCACGGATCTATAGGCTTAAAATTGAAATTTTAGGGTTACATTTGACATAAAATTCAAATTTATATATTATAGATCGATTAGTTTTTAAAAAATTCGAAAATGCCAATCACCTATTAGACACAAAATTGAAAGTTCATGACCTATTAGCAATTTTTAACATTATGGGATATTTAGACACATTTGAAAGTTCAAGGCCAAATAGATACAAACCTTAAAGTTTATGACTAAATTTATAGTCTAACCGTAACCGATTTGTTTGTTTGTTTGTTTGTTCCTATGATGTGTCTGCTTCTCATGAGTCTAGCGTTGCTAATTCTGTATCTTCCTCTTTTGATCATCTGTGTGAATCATTCCGGTTTCAGCTTTATACAAAACCTATGAGGTTAAATGCTTTCTTTTGTTCAGGGACCTCTTGTGCAGCTAGAAGTTGAGGGGTCTGGGTTCTTGTATAGACAAGTTCGGAATATGGTAATTATTTTTGTTACATTTATCTTTTCAGTCAAAAGTCCCTTGTCTGATTATTTTATTTTTACTTGTGTTGGTTGCTAAAATGGCTTTATAGTAGAAAGTAAACTTCCTGGAAGCAGAGATTTAAGTTTGTAAAATCTGTTTCATGCAACTCAGGAACTGTAAGCATTTTCTCTAGTGCTTTTGCAGGTTGGCTTGTTGGTTCAAATTGGAAAGGAAGCAATTCCTCCTGATGTTGTTCCTAAGATTTTGGCTTCTCGAGATCGCAAGGAGCTTGCCAAATATGCGTCAGGCGTTCCTCCTCAGGGGCTGTGCCTTGTGAGTATTAATTATAAAGAGGACCATTTAAAGCTTCCATCTGATTGCCCTGGGACTAGTTTTGGTAGGAGTCGTAGTGTGAGCAAATGTAAACTCCCTTTTTATTGA

mRNA sequence

ATGAGGTGCTGGGCGGTGGGTTCGCCTGTTCTTCCTCCCATTTCCCTTCCCCTTTCACCAAGCATTCCATTTCCAAATGGCGCTTCATCTTCCTCCAGTCGTGGATTCCCGAATGTGGAGGATTCCTCTTATATCACAGAGCCCCCTTTCCCCAGCTTCAAATGGCGGTTGCTCATCGCTTATGATGGTACCCGATACGCAGGCTGGCAATATCAGCGGTCACCACCCACTATTCAGTGCCTCATCGAAGAAGCCTTGATTGGAGCAACAAAGCTTGAAAGGAAGGATCTCCATTTAGTTGGTGCAAGCAGAACAGATAAAGGAGTTCATGCCTGGGGTCAGGTGGCTCACTTTATTACGCCATTTAACTACGATAGCTTGGAAAACGTTCATGCTGCACTGAATGGTCTTCTTCCCGTGGATATAAGAGTTCGAGAGATCAGTCCTGCTGCGCCTGACTTTCATGCTCGGTTTTCTACGTTAAGCAAGGTTTATCACTACAAGATATATAATGGCGCAATCTTGGATCCATTTCAGCGTTGCTACACTTACCATTGTGTTTATAAACTCAACTATGCAGCCATGAGAGAAGCTGCAAATTATTTTGTTGGGAAGCATGATTTTTCTGCTTTTACAAATGCAGCACATAATGATCGAGTGGTAAATCCGGTGAAGGTCATTTTTCGTTTTGATGTCATAGAAATGGGACCTCTTGTGCAGCTAGAAGTTGAGGGGTCTGGGTTCTTGTATAGACAAGTTCGGAATATGGTTGGCTTGTTGGTTCAAATTGGAAAGGAAGCAATTCCTCCTGATGTTGTTCCTAAGATTTTGGCTTCTCGAGATCGCAAGGAGCTTGCCAAATATGCGTCAGGCGTTCCTCCTCAGGGGCTGTGCCTTGTGAGTATTAATTATAAAGAGGACCATTTAAAGCTTCCATCTGATTGCCCTGGGACTAGTTTTGGTAGGAGTCGTAGTGTGAGCAAATGTAAACTCCCTTTTTATTGA

Coding sequence (CDS)

ATGAGGTGCTGGGCGGTGGGTTCGCCTGTTCTTCCTCCCATTTCCCTTCCCCTTTCACCAAGCATTCCATTTCCAAATGGCGCTTCATCTTCCTCCAGTCGTGGATTCCCGAATGTGGAGGATTCCTCTTATATCACAGAGCCCCCTTTCCCCAGCTTCAAATGGCGGTTGCTCATCGCTTATGATGGTACCCGATACGCAGGCTGGCAATATCAGCGGTCACCACCCACTATTCAGTGCCTCATCGAAGAAGCCTTGATTGGAGCAACAAAGCTTGAAAGGAAGGATCTCCATTTAGTTGGTGCAAGCAGAACAGATAAAGGAGTTCATGCCTGGGGTCAGGTGGCTCACTTTATTACGCCATTTAACTACGATAGCTTGGAAAACGTTCATGCTGCACTGAATGGTCTTCTTCCCGTGGATATAAGAGTTCGAGAGATCAGTCCTGCTGCGCCTGACTTTCATGCTCGGTTTTCTACGTTAAGCAAGGTTTATCACTACAAGATATATAATGGCGCAATCTTGGATCCATTTCAGCGTTGCTACACTTACCATTGTGTTTATAAACTCAACTATGCAGCCATGAGAGAAGCTGCAAATTATTTTGTTGGGAAGCATGATTTTTCTGCTTTTACAAATGCAGCACATAATGATCGAGTGGTAAATCCGGTGAAGGTCATTTTTCGTTTTGATGTCATAGAAATGGGACCTCTTGTGCAGCTAGAAGTTGAGGGGTCTGGGTTCTTGTATAGACAAGTTCGGAATATGGTTGGCTTGTTGGTTCAAATTGGAAAGGAAGCAATTCCTCCTGATGTTGTTCCTAAGATTTTGGCTTCTCGAGATCGCAAGGAGCTTGCCAAATATGCGTCAGGCGTTCCTCCTCAGGGGCTGTGCCTTGTGAGTATTAATTATAAAGAGGACCATTTAAAGCTTCCATCTGATTGCCCTGGGACTAGTTTTGGTAGGAGTCGTAGTGTGAGCAAATGTAAACTCCCTTTTTATTGA

Protein sequence

MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPNVEDSSYITEPPFPSFKWRLLIAYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFITPFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQRCYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGPLVQLEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQGLCLVSINYKEDHLKLPSDCPGTSFGRSRSVSKCKLPFY
Homology
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BLAST of HG10010458 vs. NCBI nr
Match: XP_038875516.1 (tRNA pseudouridine synthase A 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 670.6 bits (1729), Expect = 6.9e-189
Identity = 321/334 (96.11%), Postives = 328/334 (98.20%), Query Frame = 0 

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPNVEDSSYITEPPFPSFKWRLLIA 60
           MRCWAVGSPVLPPISLPLSP+IPFPNGASSSSSRG PN EDSS I  PPFPSFKWRLLIA
Sbjct: 1   MRCWAVGSPVLPPISLPLSPAIPFPNGASSSSSRGSPNAEDSSCIIAPPFPSFKWRLLIA 60

Query: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120
           YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120

Query: 121 PFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR 180
           PFNYDSLE+VHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR
Sbjct: 121 PFNYDSLESVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR 180

Query: 181 CYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGPLVQ 240
           CYTYHCVYKLNYAAM+EAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI+MGPLVQ
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIDMGPLVQ 240

Query: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQGLCLV 300
           LEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILAS+DR+ELAKYASGVPPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASQDRRELAKYASGVPPQGLCLV 300

Query: 301 SINYKEDHLKLPSDCPGTSFGRSRSVSKCKLPFY 335
           SINYKEDHLKLPSDCPGTS GR+RSVSKCKLPFY
Sbjct: 301 SINYKEDHLKLPSDCPGTSVGRNRSVSKCKLPFY 334

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BLAST of HG10010458 vs. NCBI nr
Match: XP_008458279.1 (PREDICTED: tRNA pseudouridine synthase A 1 isoform X1 [Cucumis melo])

HSP 1 Score: 667.5 bits (1721), Expect = 5.8e-188
Identity = 317/334 (94.91%), Postives = 326/334 (97.60%), Query Frame = 0 

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPNVEDSSYITEPPFPSFKWRLLIA 60
           MRCWAVGSPVLPPISLP SP IP PNGASSSSSRGFPN EDSSYITEPPFPSFKWRLLIA
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA 60

Query: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120
           YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHF+T
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT 120

Query: 121 PFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR 180
           PFNYDSLE+VHAALNGLLPVDIRVRE+SPAAPDFHARFSTLSKVYHYKIYNG I+DPFQR
Sbjct: 121 PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR 180

Query: 181 CYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGPLVQ 240
           CYTYHCVYKLNYAAM+EAANYFVGKHDFSAFTNAAHN+RVVNPVKVIFRFDVIEMG LVQ
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ 240

Query: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQGLCLV 300
           LEVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILASRDRKELAKYASGVPPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV 300

Query: 301 SINYKEDHLKLPSDCPGTSFGRSRSVSKCKLPFY 335
           SINYKEDHLKLPSDCPGTS GRSRSV KCK+PFY
Sbjct: 301 SINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 334

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BLAST of HG10010458 vs. NCBI nr
Match: XP_004138518.2 (uncharacterized protein LOC101205797 [Cucumis sativus] >KGN45627.2 hypothetical protein Csa_005280 [Cucumis sativus])

HSP 1 Score: 656.0 bits (1691), Expect = 1.8e-184
Identity = 313/335 (93.43%), Postives = 325/335 (97.01%), Query Frame = 0 

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPNVE-DSSYITEPPFPSFKWRLLI 60
           MRCWAVGSPVLPPISLPLSP IP PNGASSSSSRGFPN E D+SYI EP FP FKWRLLI
Sbjct: 21  MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 80

Query: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFI 120
           AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHF+
Sbjct: 81  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 140

Query: 121 TPFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQ 180
           TPFNYDSLE+VHAALNGLLPVDIRVRE+SPA PDFHARFSTLSKVYHYKIY+G I+DPFQ
Sbjct: 141 TPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 200

Query: 181 RCYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGPLV 240
           RCYTYHCVYKLNYAAM+EAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMG LV
Sbjct: 201 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 260

Query: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQGLCL 300
           QLEVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILASRDR+ELAKYASGVPPQGLCL
Sbjct: 261 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 320

Query: 301 VSINYKEDHLKLPSDCPGTSFGRSRSVSKCKLPFY 335
           VSINYKEDHLKLPSDCPGTSFGR+RSVSKCK+PFY
Sbjct: 321 VSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 355

[Close]
BLAST of HG10010458 vs. NCBI nr
Match: XP_038875517.1 (tRNA pseudouridine synthase A 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 645.2 bits (1663), Expect = 3.1e-181
Identity = 312/334 (93.41%), Postives = 318/334 (95.21%), Query Frame = 0 

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPNVEDSSYITEPPFPSFKWRLLIA 60
           MRCWAVGSPVLPPISLPLSP+IPFPNGASSSSSRG PN EDSS I  PPFPSFKWRLLIA
Sbjct: 1   MRCWAVGSPVLPPISLPLSPAIPFPNGASSSSSRGSPNAEDSSCIIAPPFPSFKWRLLIA 60

Query: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120
           YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120

Query: 121 PFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR 180
           PFNYDSLE+VHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR
Sbjct: 121 PFNYDSLESVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR 180

Query: 181 CYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGPLVQ 240
           CYTYHCVYKLNYAAM+EAANYFVGKHDFSAFTNAAHNDRVVNPVK          GPLVQ
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVK----------GPLVQ 240

Query: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQGLCLV 300
           LEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILAS+DR+ELAKYASGVPPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASQDRRELAKYASGVPPQGLCLV 300

Query: 301 SINYKEDHLKLPSDCPGTSFGRSRSVSKCKLPFY 335
           SINYKEDHLKLPSDCPGTS GR+RSVSKCKLPFY
Sbjct: 301 SINYKEDHLKLPSDCPGTSVGRNRSVSKCKLPFY 324

[Close]
BLAST of HG10010458 vs. NCBI nr
Match: TYK02989.1 (protein TRIGALACTOSYLDIACYLGLYCEROL 4 [Cucumis melo var. makuwa])

HSP 1 Score: 643.3 bits (1658), Expect = 1.2e-180
Identity = 306/321 (95.33%), Postives = 314/321 (97.82%), Query Frame = 0 

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPNVEDSSYITEPPFPSFKWRLLIA 60
           MRCWAVGSPVLPPISLP SP IP PNGASSSSSRGFPN EDSSYITEPPFPSFKWRLLIA
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA 60

Query: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120
           YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHF+T
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT 120

Query: 121 PFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR 180
           PFNYDSLE+VHAALNGLLPVDIRVRE+SPAAPDFHARFSTLSKVYHYKIYNG I+DPFQR
Sbjct: 121 PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR 180

Query: 181 CYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGPLVQ 240
           CYTYHCVYKLNYAAM+EAANYFVGKHDFSAFTNAAHN+RVVNPVKVIFRFDVIEMG LVQ
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ 240

Query: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQGLCLV 300
           LEVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILASRDRKELAKYASGVPPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV 300

Query: 301 SINYKEDHLKLPSDCPGTSFG 322
           SINYKEDHLKLPSDCPGTS G
Sbjct: 301 SINYKEDHLKLPSDCPGTSLG 321

[Close]
BLAST of HG10010458 vs. ExPASy Swiss-Prot
Match: Q6MEE7 (tRNA pseudouridine synthase A 1 OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=truA1 PE=3 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.2e-53
Identity = 104/255 (40.78%), Postives = 164/255 (64.31%), Query Frame = 0 

Query: 55  WRLLIAYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQ 114
           ++L IAYDGT Y+GWQ Q +  +IQ  I+EAL     L+++ + LVG+ RTD GVHA GQ
Sbjct: 4   YKLTIAYDGTNYSGWQIQPNASSIQQKIQEAL--CILLKKEKVVLVGSGRTDAGVHAKGQ 63

Query: 115 VAHFITPFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAI 174
           VAHF    +Y  L  +H +LNGLLP DIR++ + P +P FH+++S + K YHY ++   +
Sbjct: 64  VAHFHFQ-DYIDLSRLHVSLNGLLPRDIRIKAVEPVSPRFHSQYSAIRKEYHYYLHLNKV 123

Query: 175 LDPFQRCYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVV-NPVKVIFRFDVI 234
           +DPFQR Y++H   K++   +++AA  F G HDF++F N AH      NPV+ ++R D+ 
Sbjct: 124 MDPFQRLYSWHFQRKIDVNILKKAAILFTGTHDFTSFANEAHRGTAAKNPVRTLYRLDIK 183

Query: 235 EMGPLVQLEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVP 294
                ++LE EG GFLY+ VRN+VG L+ +       + + +I A+++R++ +  A   P
Sbjct: 184 PNEGGLRLEFEGDGFLYKMVRNIVGTLMDVASHKRAIEEINQIFAAKNRRQASLAA---P 243

Query: 295 PQGLCLVSINYKEDH 309
           P+GL L+ + Y+ ++
Sbjct: 244 PEGLFLIQVFYENEN 252

[Close]
BLAST of HG10010458 vs. ExPASy Swiss-Prot
Match: B1I1B5 (tRNA pseudouridine synthase A OS=Desulforudis audaxviator (strain MP104C) OX=477974 GN=truA PE=3 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.9e-48
Identity = 109/251 (43.43%), Postives = 143/251 (56.97%), Query Frame = 0 

Query: 56  RLLIAYDGTRYAGWQYQRSP--PTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWG 115
           RL +AYDGT Y G+Q Q     PTIQ  +E  L    +L    L + GASRTD GVHA G
Sbjct: 5   RLTLAYDGTAYHGFQKQSGSGLPTIQETLERCL---AELSGAALKVTGASRTDAGVHARG 64

Query: 116 QVAHFITPFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGA 175
           QV +F+T       E + AALNG+LP DI   +      DFHAR+S ++K Y Y +YN  
Sbjct: 65  QVVNFVTGRWGIPTERIPAALNGVLPGDIAALDAREVPADFHARYSAVAKTYSYTLYNHP 124

Query: 176 ILDPFQRCYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI 235
           +  PFQR Y+ H    L+  AMR AA + +G+HDFS F  A     V + V+ + R D+ 
Sbjct: 125 VRSPFQRLYSLHVPRALDLRAMRLAARHLMGRHDFSTFQAAGR--PVQSAVRTLTRADIE 184

Query: 236 EMGPLVQLEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVP 295
              PLV+L     GFLY  VR M G L+++G E + P+ +P I+AS DR   A+    VP
Sbjct: 185 AQAPLVRLVFRADGFLYNMVRIMAGTLLEVGLERLDPEALPDIIASGDR---ARAGPTVP 244

Query: 296 PQGLCLVSINY 305
           P GLCL  + Y
Sbjct: 245 PHGLCLEEVEY 247

[Close]
BLAST of HG10010458 vs. ExPASy Swiss-Prot
Match: Q8R7Y7 (tRNA pseudouridine synthase A OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=truA PE=3 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 1.0e-46
Identity = 110/248 (44.35%), Postives = 146/248 (58.87%), Query Frame = 0 

Query: 57  LLIAYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVA 116
           L+I YDGT Y GWQYQ++  T+Q ++E+A+    K+  + ++L+G+SRTD GVHA  QVA
Sbjct: 2   LIIEYDGTNYHGWQYQKNAITVQEVLEKAI---EKVTGEKVNLIGSSRTDTGVHALYQVA 61

Query: 117 HFITPFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILD 176
           +F T       E +  ALN +LP D+ V +       FHAR+S  SK Y Y I N     
Sbjct: 62  NFKTKTKIPP-EKLPYALNAVLPDDVVVVKAKEVDKSFHARYSARSKRYRYLILNRQFPM 121

Query: 177 PFQRCYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMG 236
           P  R Y +H  Y L+  AM++AA +F G HDFSAF   A  ++ VNPVK IF   V + G
Sbjct: 122 PTMRNYCWHVPYPLDVEAMKKAAFFFRGTHDFSAF--KASGNKKVNPVKTIFDLTVEKEG 181

Query: 237 PLVQLEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQG 296
            L+++EVE  GFLY  VR +VG LV +G   I    +P I+ S+DR    K     PPQG
Sbjct: 182 DLIKIEVEADGFLYNMVRIIVGTLVYVGLGKIKAGEIPAIIESKDR---TKAGITAPPQG 240

Query: 297 LCLVSINY 305
           L LV I Y
Sbjct: 242 LYLVRIVY 240

[Close]
BLAST of HG10010458 vs. ExPASy Swiss-Prot
Match: A4J151 (tRNA pseudouridine synthase A OS=Desulfotomaculum reducens (strain MI-1) OX=349161 GN=truA PE=3 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 2.0e-45
Identity = 103/251 (41.04%), Postives = 147/251 (58.57%), Query Frame = 0 

Query: 56  RLLIAYDGTRYAGWQYQRSP--PTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWG 115
           +L +AYDGT Y G+Q QR     TIQ  +E+AL   + + +  + ++GA RTD GVHA G
Sbjct: 5   KLTLAYDGTNYHGFQEQRGTGLATIQEALEKAL---STIAQTPIQVIGAGRTDAGVHAQG 64

Query: 116 QVAHFITPFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGA 175
           QV +F +       E    ALN LLP DI+V +      DFHARFS ++K Y Y IY+  
Sbjct: 65  QVVNFRSEKWPVPAEKAPLALNVLLPGDIKVVKAEEVPMDFHARFSAVAKTYRYSIYHHR 124

Query: 176 ILDPFQRCYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI 235
           ++ PF R Y YH   +L+  AM+E A Y +G +DF +F   A    V + ++ I+R D+I
Sbjct: 125 VMSPFHRYYCYHEPRRLDVNAMQEGAAYLLGTYDFKSF--QAQGTPVKDTIRTIYRADII 184

Query: 236 EMGPLVQLEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVP 295
           E  P++ L + G+GFLY  VR + G L+ IG   I P+ + KI+ S++R      A   P
Sbjct: 185 EDAPVINLYLRGNGFLYNMVRIITGTLLNIGFGKIKPEDMVKIIESKNRTLAGTTA---P 244

Query: 296 PQGLCLVSINY 305
           PQGLCL+ + Y
Sbjct: 245 PQGLCLMEVEY 247

[Close]
BLAST of HG10010458 vs. ExPASy Swiss-Prot
Match: A3DJK8 (tRNA pseudouridine synthase A OS=Hungateiclostridium thermocellum (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=truA PE=3 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 2.0e-45
Identity = 103/249 (41.37%), Postives = 144/249 (57.83%), Query Frame = 0 

Query: 56  RLLIAYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQV 115
           +L+I YDGT Y GWQ Q++  T+Q  IE A+ G T    + + L+GASRTD GVHA GQV
Sbjct: 5   KLIIEYDGTNYHGWQKQKNAKTVQDTIERAIKGLT---GEKVDLIGASRTDFGVHALGQV 64

Query: 116 AHFITPFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAIL 175
           A+FIT       +    ALN +LP DI ++E      DFHAR+    K Y Y IYN    
Sbjct: 65  ANFITNSGIPG-DRFSYALNRMLPDDIVIKESQEVDMDFHARYKAKGKRYRYLIYNSQFP 124

Query: 176 DPFQRCYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEM 235
               R  TYH  +KL++ +M+ AA+YF+G HDFSAF ++  + +    V+ I    +   
Sbjct: 125 SALLRHRTYHVSHKLDFESMQRAASYFLGTHDFSAFRSSGSSAK--TSVRTITDVSLERE 184

Query: 236 GPLVQLEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQ 295
             +V+ E+ G GFLY  VR +VG L+++G   I  D +P+I+ SR RK   + A   P +
Sbjct: 185 DKIVKFEIAGDGFLYNMVRIIVGTLMEVGIGKIRADEIPQIIESRKRKRAGRTA---PAE 244

Query: 296 GLCLVSINY 305
           GL LV + Y
Sbjct: 245 GLYLVEVYY 244

[Close]
BLAST of HG10010458 vs. ExPASy TrEMBL
Match: A0A1S3C7L6 (tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497746 PE=3 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 2.8e-188
Identity = 317/334 (94.91%), Postives = 326/334 (97.60%), Query Frame = 0 

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPNVEDSSYITEPPFPSFKWRLLIA 60
           MRCWAVGSPVLPPISLP SP IP PNGASSSSSRGFPN EDSSYITEPPFPSFKWRLLIA
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA 60

Query: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120
           YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHF+T
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT 120

Query: 121 PFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR 180
           PFNYDSLE+VHAALNGLLPVDIRVRE+SPAAPDFHARFSTLSKVYHYKIYNG I+DPFQR
Sbjct: 121 PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR 180

Query: 181 CYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGPLVQ 240
           CYTYHCVYKLNYAAM+EAANYFVGKHDFSAFTNAAHN+RVVNPVKVIFRFDVIEMG LVQ
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ 240

Query: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQGLCLV 300
           LEVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILASRDRKELAKYASGVPPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV 300

Query: 301 SINYKEDHLKLPSDCPGTSFGRSRSVSKCKLPFY 335
           SINYKEDHLKLPSDCPGTS GRSRSV KCK+PFY
Sbjct: 301 SINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 334

[Close]
BLAST of HG10010458 vs. ExPASy TrEMBL
Match: A0A5D3BY40 (Protein TRIGALACTOSYLDIACYLGLYCEROL 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00780 PE=3 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 5.7e-181
Identity = 306/321 (95.33%), Postives = 314/321 (97.82%), Query Frame = 0 

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPNVEDSSYITEPPFPSFKWRLLIA 60
           MRCWAVGSPVLPPISLP SP IP PNGASSSSSRGFPN EDSSYITEPPFPSFKWRLLIA
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA 60

Query: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120
           YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHF+T
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT 120

Query: 121 PFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR 180
           PFNYDSLE+VHAALNGLLPVDIRVRE+SPAAPDFHARFSTLSKVYHYKIYNG I+DPFQR
Sbjct: 121 PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR 180

Query: 181 CYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGPLVQ 240
           CYTYHCVYKLNYAAM+EAANYFVGKHDFSAFTNAAHN+RVVNPVKVIFRFDVIEMG LVQ
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ 240

Query: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQGLCLV 300
           LEVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILASRDRKELAKYASGVPPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV 300

Query: 301 SINYKEDHLKLPSDCPGTSFG 322
           SINYKEDHLKLPSDCPGTS G
Sbjct: 301 SINYKEDHLKLPSDCPGTSLG 321

[Close]
BLAST of HG10010458 vs. ExPASy TrEMBL
Match: A0A0A0KB67 (tRNA pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_6G001730 PE=3 SV=1)

HSP 1 Score: 631.3 bits (1627), Expect = 2.2e-177
Identity = 302/322 (93.79%), Postives = 312/322 (96.89%), Query Frame = 0 

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPNVE-DSSYITEPPFPSFKWRLLI 60
           MRCWAVGSPVLPPISLPLSP IP PNGASSSSSRGFPN E D+SYI EP FP FKWRLLI
Sbjct: 1   MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 60

Query: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFI 120
           AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHF+
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120

Query: 121 TPFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQ 180
           TPFNYDSLE+VHAALNGLLPVDIRVRE+SPA PDFHARFSTLSKVYHYKIY+G I+DPFQ
Sbjct: 121 TPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 180

Query: 181 RCYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGPLV 240
           RCYTYHCVYKLNYAAM+EAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMG LV
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 240

Query: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQGLCL 300
           QLEVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILASRDR+ELAKYASGVPPQGLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 300

Query: 301 VSINYKEDHLKLPSDCPGTSFG 322
           VSINYKEDHLKLPSDCPGTSFG
Sbjct: 301 VSINYKEDHLKLPSDCPGTSFG 322

[Close]
BLAST of HG10010458 vs. ExPASy TrEMBL
Match: A0A6J1L0L1 (tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499282 PE=3 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 6.3e-172
Identity = 293/334 (87.72%), Postives = 305/334 (91.32%), Query Frame = 0 

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPNVEDSSYITEPPFPSFKWRLLIA 60
           M CWAVGS VLP +  PLSPSIP   G  SS+ RGFPN EDSS + EP FPSFKWRL+IA
Sbjct: 1   MSCWAVGSAVLPSVPRPLSPSIP-SRGTLSSTGRGFPNSEDSSCVPEPDFPSFKWRLVIA 60

Query: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120
           YDGTRYAGWQYQRSPPTIQCLIEEAL GAT+LERKDLHLVGASRTDKGVHAWGQVAHFIT
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALTGATRLERKDLHLVGASRTDKGVHAWGQVAHFIT 120

Query: 121 PFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR 180
           PFNYDSL+ +HAALNGLLP DIRVREISPA PDFHARFSTLSKVYHYKIYN AILDPFQR
Sbjct: 121 PFNYDSLDRIHAALNGLLPADIRVREISPAVPDFHARFSTLSKVYHYKIYNDAILDPFQR 180

Query: 181 CYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGPLVQ 240
           CYTYHCVYKLNYAAM+EAANYFVGKHDFSAF N AHNDRVVNPVKVIFRFDVIEMG L+Q
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGALIQ 240

Query: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQGLCLV 300
           LEVEGSGFLYRQVRNMV LL+QIGKEAIPPDVVPKILASRDRK LAKY + +PPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVALLLQIGKEAIPPDVVPKILASRDRKVLAKYTTCIPPQGLCLV 300

Query: 301 SINYKEDHLKLPSDCPGTSFGRSRSVSKCKLPFY 335
            INYKEDHLKLPSDCPGTSFGRS SVSKCKLPFY
Sbjct: 301 RINYKEDHLKLPSDCPGTSFGRSHSVSKCKLPFY 333

[Close]
BLAST of HG10010458 vs. ExPASy TrEMBL
Match: A0A6J1L2M8 (tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499282 PE=3 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 2.0e-170
Identity = 293/336 (87.20%), Postives = 305/336 (90.77%), Query Frame = 0 

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFP--NVEDSSYITEPPFPSFKWRLL 60
           M CWAVGS VLP +  PLSPSIP   G  SS+ RGFP  N EDSS + EP FPSFKWRL+
Sbjct: 1   MSCWAVGSAVLPSVPRPLSPSIP-SRGTLSSTGRGFPVMNSEDSSCVPEPDFPSFKWRLV 60

Query: 61  IAYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHF 120
           IAYDGTRYAGWQYQRSPPTIQCLIEEAL GAT+LERKDLHLVGASRTDKGVHAWGQVAHF
Sbjct: 61  IAYDGTRYAGWQYQRSPPTIQCLIEEALTGATRLERKDLHLVGASRTDKGVHAWGQVAHF 120

Query: 121 ITPFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPF 180
           ITPFNYDSL+ +HAALNGLLP DIRVREISPA PDFHARFSTLSKVYHYKIYN AILDPF
Sbjct: 121 ITPFNYDSLDRIHAALNGLLPADIRVREISPAVPDFHARFSTLSKVYHYKIYNDAILDPF 180

Query: 181 QRCYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGPL 240
           QRCYTYHCVYKLNYAAM+EAANYFVGKHDFSAF N AHNDRVVNPVKVIFRFDVIEMG L
Sbjct: 181 QRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGAL 240

Query: 241 VQLEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQGLC 300
           +QLEVEGSGFLYRQVRNMV LL+QIGKEAIPPDVVPKILASRDRK LAKY + +PPQGLC
Sbjct: 241 IQLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDVVPKILASRDRKVLAKYTTCIPPQGLC 300

Query: 301 LVSINYKEDHLKLPSDCPGTSFGRSRSVSKCKLPFY 335
           LV INYKEDHLKLPSDCPGTSFGRS SVSKCKLPFY
Sbjct: 301 LVRINYKEDHLKLPSDCPGTSFGRSHSVSKCKLPFY 335

[Close]
BLAST of HG10010458 vs. TAIR 10
Match: AT3G06950.1 (Pseudouridine synthase family protein )

HSP 1 Score: 427.6 bits (1098), Expect = 9.5e-120
Identity = 210/328 (64.02%), Postives = 248/328 (75.61%), Query Frame = 0 

Query: 8   SPVLPPISLPLSPSIPFPNGASSSSSRGFPNVEDSSYITEPPFPSFKWRLLIAYDGTRYA 67
           +P LPP SL  +  +  P   S ++      V DS         ++KWRL+IAYDGTR+A
Sbjct: 5   TPALPP-SLFTNGYLSSPQNISMTNLNSSVKVSDSG--------AYKWRLVIAYDGTRFA 64

Query: 68  GWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFITPFNYDSL 127
           GWQYQ SPPTIQ ++E+ALI  T+L RK+L L+GA RTD GVHAWGQVAHF+TPFNY SL
Sbjct: 65  GWQYQESPPTIQSMLEKALIQITELGRKELQLIGAGRTDAGVHAWGQVAHFVTPFNYTSL 124

Query: 128 ENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQRCYTYHCV 187
           ++ HAALNGLLP DIRVRE+S A P+FHARFS  SKVY Y+IYN   +DPFQR + YHC 
Sbjct: 125 DSFHAALNGLLPKDIRVRELSAAVPEFHARFSASSKVYRYQIYNDTFMDPFQRHWAYHCA 184

Query: 188 YKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGPLVQLEVEGSG 247
           YKLN + MREAAN FVGKHDFSAF NA   D V +P+K I RFDVI+MG L+QLEVEGSG
Sbjct: 185 YKLNASKMREAANLFVGKHDFSAFANATREDGVPDPLKTISRFDVIQMGSLLQLEVEGSG 244

Query: 248 FLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYAS-GVPPQGLCLVSINYKE 307
           FLYRQVRNMV LL+QIGKEA+  D+VP IL ++DR+ LAKY S    P GLCLVS+ YKE
Sbjct: 245 FLYRQVRNMVALLIQIGKEALDSDIVPMILETKDRRVLAKYTSLPASPHGLCLVSVKYKE 304

Query: 308 DHLKLPSDCPGTSFGRSRSVSKCKLPFY 335
           DHLKLP DCP TS GR  +++KCKLPFY
Sbjct: 305 DHLKLPLDCPVTSSGRHHTITKCKLPFY 323

[Close]
BLAST of HG10010458 vs. TAIR 10
Match: AT1G34150.1 (Pseudouridine synthase family protein )

HSP 1 Score: 82.8 bits (203), Expect = 5.7e-16
Identity = 80/268 (29.85%), Postives = 110/268 (41.04%), Query Frame = 0 

Query: 57  LLIAYDGTRYAGWQYQ-RSPPTIQCLIEEALIGATKL--ERKDLHLVGASRTDKGVHAWG 116
           L I Y G R+ G+  + +  P+I+  + +AL     L  ++KD       RTDKGV + G
Sbjct: 94  LKIMYFGKRFYGFSAEAQMEPSIESEVFKALERTRLLVGDKKDSCYSRCGRTDKGVSSTG 153

Query: 117 QVAHFITPFNY-----DSLENVHA------------ALNGLLPVDIRVREISPAAPDFHA 176
           QV              DS   V+              LN  LP DIRV   SPA  DFHA
Sbjct: 154 QVIALFLRSRLKSPPGDSKAQVNGRTGERPEYDYVRVLNRALPDDIRVIGWSPAPIDFHA 213

Query: 177 RFSTLSKVYHYKIYNGAILDPFQRCYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAH 236
           RFS  ++ Y Y  +                   LN +AM  A   F+G+HDF  F     
Sbjct: 214 RFSCYAREYKYFFWR----------------QNLNLSAMDFAGKKFIGEHDFRNFCKM-- 273

Query: 237 NDRVVNPVKVIFRFDVIEMGP---------LVQLEVEGSGFLYRQVRNMVGLLVQIGKEA 294
              V N      R    E+ P         L    + GS FL+ Q+R MV +L  IG+  
Sbjct: 274 --DVANVHCYTRRVTFFEVSPCQNSHEGDQLCTFTMRGSAFLWHQIRCMVAVLFMIGQGV 333

The following BLAST results are available for this feature:
BLAST of HG10010458 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038875516.16.9e-18996.11tRNA pseudouridine synthase A 1 isoform X1 [Benincasa hispida][more]
XP_008458279.15.8e-18894.91PREDICTED: tRNA pseudouridine synthase A 1 isoform X1 [Cucumis melo][more]
XP_004138518.21.8e-18493.43uncharacterized protein LOC101205797 [Cucumis sativus] >KGN45627.2 hypothetical ... [more]
XP_038875517.13.1e-18193.41tRNA pseudouridine synthase A 1 isoform X2 [Benincasa hispida][more]
TYK02989.11.2e-18095.33protein TRIGALACTOSYLDIACYLGLYCEROL 4 [Cucumis melo var. makuwa][more]
BLAST of HG10010458 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q6MEE71.2e-5340.78tRNA pseudouridine synthase A 1 OS=Protochlamydia amoebophila (strain UWE25) OX=... [more]
B1I1B51.9e-4843.43tRNA pseudouridine synthase A OS=Desulforudis audaxviator (strain MP104C) OX=477... [more]
Q8R7Y71.0e-4644.35tRNA pseudouridine synthase A OS=Caldanaerobacter subterraneus subsp. tengcongen... [more]
A4J1512.0e-4541.04tRNA pseudouridine synthase A OS=Desulfotomaculum reducens (strain MI-1) OX=3491... [more]
A3DJK82.0e-4541.37tRNA pseudouridine synthase A OS=Hungateiclostridium thermocellum (strain ATCC 2... [more]
BLAST of HG10010458 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S3C7L62.8e-18894.91tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497746 PE=3 SV=1[more]
A0A5D3BY405.7e-18195.33Protein TRIGALACTOSYLDIACYLGLYCEROL 4 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A0A0KB672.2e-17793.79tRNA pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_6G001730 PE=3 SV=1[more]
A0A6J1L0L16.3e-17287.72tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499282 PE=3 SV=... [more]
A0A6J1L2M82.0e-17087.20tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499282 PE=3 SV=... [more]
BLAST of HG10010458 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G06950.19.5e-12064.02Pseudouridine synthase family protein [more]
AT1G34150.15.7e-1629.85Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020095Pseudouridine synthase I, TruA, C-terminalGENE3D3.30.70.660coord: 161..303
e-value: 1.2E-39
score: 137.2
IPR020097Pseudouridine synthase I, TruA, alpha/beta domainPFAMPF01416PseudoU_synth_1coord: 60..158
e-value: 2.2E-5
score: 24.9
coord: 198..304
e-value: 3.2E-25
score: 88.7
IPR020094Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminalGENE3D3.30.70.580coord: 52..160
e-value: 4.7E-29
score: 102.8
IPR001406Pseudouridine synthase I, TruATIGRFAMTIGR00071TIGR00071coord: 57..289
e-value: 2.8E-57
score: 192.0
IPR001406Pseudouridine synthase I, TruAPANTHERPTHR11142PSEUDOURIDYLATE SYNTHASEcoord: 49..317
IPR001406Pseudouridine synthase I, TruAHAMAPMF_00171TruAcoord: 53..304
score: 32.077843
IPR001406Pseudouridine synthase I, TruACDDcd02570PseudoU_synth_EcTruAcoord: 57..304
e-value: 4.08291E-110
score: 318.263
NoneNo IPR availablePIRSRPIRSR001430-2PIRSR001430-2coord: 94..299
e-value: 1.6E-62
score: 208.5
NoneNo IPR availablePANTHERPTHR11142:SF0TRNA PSEUDOURIDINE SYNTHASE-LIKE 1coord: 49..317
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 53..310

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10010458.1HG10010458.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034196 acylglycerol transport
biological_process GO:1990052 ER to chloroplast lipid transport
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0008033 tRNA processing
biological_process GO:0009451 RNA modification
molecular_function GO:0070300 phosphatidic acid binding
molecular_function GO:0003723 RNA binding
molecular_function GO:0106029 tRNA pseudouridine synthase activity
molecular_function GO:0009982 pseudouridine synthase activity