HG10010417 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10010417
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionUBC core domain-containing protein
LocationChr06: 22010389 .. 22011306 (+)
RNA-Seq ExpressionHG10010417
SyntenyHG10010417
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCTCCTCCTCTCTGCTATTCACACGACGACAAAGACAAACATCCGGCCACCATAGACGGTGGTGACGAACCTGAGAGATCGCCTCCGCCTTCGTTCTCCTTCAAACGGTTCGATATCGTCACCGACTACTCTGATCACCATTACGCCGATTCGAACCTGTTCGCGAACTGCATTGGATCGGATGTCTCAAAGAAGATCATGCGAGAATGGAAGATTCTGGAAAGAAATCTTCCGGAATCGATTTTCGTTCGTGCTTACGAAGAGAGAATCGATCTTCTAAGAGCAGTAATCGTCGGTCCAGCCGGAACTCCGTATCACGATGGATTGTTCTTCTTCGACCTAGCTTTTACTTCCGATTATCCGATTAGTCCTCCGATGGTGTATTACCATTCCTACGGACTGAGATTAAACCCTAACCTGTACGAAGACGGCTATGTGTGCTTGAGTTTGATAAATACCTGGGATGGAAAAGAAGAAGAGAGATGGAATCCAGCGATTTCGACAGTTTTACAGATTCTAATATCGATTCAAGCACTGGTGTTGAACGAGAAGCCGTATTTCAACGAACCGGAACTAGAAGAGGAAGCGGAGGCGGAGATTTACTCAGATGCATACAATAAGGAAGCGTACTTGTTCTGTTGCAAGATGATGATGAGATTAATGAAGAAAGCGCCGAAGAATTTCGAGGAATTTGTGAGGAATTACTTTGGGGAGAAAGGGAAGGTGATACTGGCGGCGTGCAAGGCTTACTCCGATGGCCGTATTAGGGTTGGGCGGTTCAATGAAAAGGAGAATAGTGAAAGTGAAAGTGATTATTGTAACAGAGCGGACGTTTCTGCCATTTTTAAGGCGTTAATGGAGAAGATGCATCGGGATCTGCACGCAGCTTTCCTCCTCATCTGCCCCTCCTAG

mRNA sequence

ATGGCTTCTCCTCCTCTCTGCTATTCACACGACGACAAAGACAAACATCCGGCCACCATAGACGGTGGTGACGAACCTGAGAGATCGCCTCCGCCTTCGTTCTCCTTCAAACGGTTCGATATCGTCACCGACTACTCTGATCACCATTACGCCGATTCGAACCTGTTCGCGAACTGCATTGGATCGGATGTCTCAAAGAAGATCATGCGAGAATGGAAGATTCTGGAAAGAAATCTTCCGGAATCGATTTTCGTTCGTGCTTACGAAGAGAGAATCGATCTTCTAAGAGCAGTAATCGTCGGTCCAGCCGGAACTCCGTATCACGATGGATTGTTCTTCTTCGACCTAGCTTTTACTTCCGATTATCCGATTAGTCCTCCGATGGTGTATTACCATTCCTACGGACTGAGATTAAACCCTAACCTGTACGAAGACGGCTATGTGTGCTTGAGTTTGATAAATACCTGGGATGGAAAAGAAGAAGAGAGATGGAATCCAGCGATTTCGACAGTTTTACAGATTCTAATATCGATTCAAGCACTGGTGTTGAACGAGAAGCCGTATTTCAACGAACCGGAACTAGAAGAGGAAGCGGAGGCGGAGATTTACTCAGATGCATACAATAAGGAAGCGTACTTGTTCTGTTGCAAGATGATGATGAGATTAATGAAGAAAGCGCCGAAGAATTTCGAGGAATTTGTGAGGAATTACTTTGGGGAGAAAGGGAAGGTGATACTGGCGGCGTGCAAGGCTTACTCCGATGGCCGTATTAGGGTTGGGCGGTTCAATGAAAAGGAGAATAGTGAAAGTGAAAGTGATTATTGTAACAGAGCGGACGTTTCTGCCATTTTTAAGGCGTTAATGGAGAAGATGCATCGGGATCTGCACGCAGCTTTCCTCCTCATCTGCCCCTCCTAG

Coding sequence (CDS)

ATGGCTTCTCCTCCTCTCTGCTATTCACACGACGACAAAGACAAACATCCGGCCACCATAGACGGTGGTGACGAACCTGAGAGATCGCCTCCGCCTTCGTTCTCCTTCAAACGGTTCGATATCGTCACCGACTACTCTGATCACCATTACGCCGATTCGAACCTGTTCGCGAACTGCATTGGATCGGATGTCTCAAAGAAGATCATGCGAGAATGGAAGATTCTGGAAAGAAATCTTCCGGAATCGATTTTCGTTCGTGCTTACGAAGAGAGAATCGATCTTCTAAGAGCAGTAATCGTCGGTCCAGCCGGAACTCCGTATCACGATGGATTGTTCTTCTTCGACCTAGCTTTTACTTCCGATTATCCGATTAGTCCTCCGATGGTGTATTACCATTCCTACGGACTGAGATTAAACCCTAACCTGTACGAAGACGGCTATGTGTGCTTGAGTTTGATAAATACCTGGGATGGAAAAGAAGAAGAGAGATGGAATCCAGCGATTTCGACAGTTTTACAGATTCTAATATCGATTCAAGCACTGGTGTTGAACGAGAAGCCGTATTTCAACGAACCGGAACTAGAAGAGGAAGCGGAGGCGGAGATTTACTCAGATGCATACAATAAGGAAGCGTACTTGTTCTGTTGCAAGATGATGATGAGATTAATGAAGAAAGCGCCGAAGAATTTCGAGGAATTTGTGAGGAATTACTTTGGGGAGAAAGGGAAGGTGATACTGGCGGCGTGCAAGGCTTACTCCGATGGCCGTATTAGGGTTGGGCGGTTCAATGAAAAGGAGAATAGTGAAAGTGAAAGTGATTATTGTAACAGAGCGGACGTTTCTGCCATTTTTAAGGCGTTAATGGAGAAGATGCATCGGGATCTGCACGCAGCTTTCCTCCTCATCTGCCCCTCCTAG

Protein sequence

MASPPLCYSHDDKDKHPATIDGGDEPERSPPPSFSFKRFDIVTDYSDHHYADSNLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTSDYPISPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPELEEEAEAEIYSDAYNKEAYLFCCKMMMRLMKKAPKNFEEFVRNYFGEKGKVILAACKAYSDGRIRVGRFNEKENSESESDYCNRADVSAIFKALMEKMHRDLHAAFLLICPS
Homology
BLAST of HG10010417 vs. NCBI nr
Match: XP_038907021.1 (probable ubiquitin-conjugating enzyme E2 25 [Benincasa hispida])

HSP 1 Score: 583.2 bits (1502), Expect = 1.3e-162
Identity = 281/303 (92.74%), Postives = 290/303 (95.71%), Query Frame = 0

Query: 2   ASPPLCYSHDDKDKHPATIDGGDEPERSPPPS-FSFKRFDIVTDYSDHHYADSNLFANCI 61
           +S PLCYSHD +DKHPAT DGGDEP  SPPP+ FSFK+FDIV+DYSDHHYADSNLFANCI
Sbjct: 5   SSSPLCYSHDGEDKHPATEDGGDEPNGSPPPTPFSFKQFDIVSDYSDHHYADSNLFANCI 64

Query: 62  GSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTS 121
           GSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTS
Sbjct: 65  GSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTS 124

Query: 122 DYPISPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILISIQA 181
           DYP SPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILISIQA
Sbjct: 125 DYPNSPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILISIQA 184

Query: 182 LVLNEKPYFNEPELEEEAEAEIYSDAYNKEAYLFCCKMMMRLMKKAPKNFEEFVRNYFGE 241
           LVLNEKPYFNEPELEEE EAEIYSDAYNKEAYLFCCKMMMRLMK  PKN+EEFV+NYFGE
Sbjct: 185 LVLNEKPYFNEPELEEEVEAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNYEEFVKNYFGE 244

Query: 242 KGKVILAACKAYSDGRIRVGRFNEKENSESESDYCNRADVSAIFKALMEKMHRDLHAAFL 301
           KGK ILAACKAYSDGR+RVGRFNEKENSESESD CNRA+VSAIFKALMEKMHRDLHAAFL
Sbjct: 245 KGKGILAACKAYSDGRVRVGRFNEKENSESESDDCNRAEVSAIFKALMEKMHRDLHAAFL 304

Query: 302 LIC 304
           L C
Sbjct: 305 LFC 307

BLAST of HG10010417 vs. NCBI nr
Match: XP_004148800.1 (probable ubiquitin-conjugating enzyme E2 25 [Cucumis sativus] >KGN47459.1 hypothetical protein Csa_021762 [Cucumis sativus])

HSP 1 Score: 520.4 bits (1339), Expect = 1.1e-143
Identity = 258/317 (81.39%), Postives = 275/317 (86.75%), Query Frame = 0

Query: 1   MASPPLCYSH---DDKDKHPATIDGGDEPERS----PPPSFSFKRFDIVTDYSDHHYADS 60
           MASP L  S    D +DKHP T+DGGDEP RS     PPSFSFKRFDIVTDYSDHHYADS
Sbjct: 1   MASPSLPPSPPPLDGEDKHPTTVDGGDEPNRSLSSPSPPSFSFKRFDIVTDYSDHHYADS 60

Query: 61  NLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFF 120
           NLF NCIGSDVSKKIMREWK+LERNLPESIFVRAYEERIDLLRAVIVGP+GTPYHDGLFF
Sbjct: 61  NLFPNCIGSDVSKKIMREWKVLERNLPESIFVRAYEERIDLLRAVIVGPSGTPYHDGLFF 120

Query: 121 FDLAFTSDYPISPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQ 180
           FDLAFTSDYP +PPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNP+ISTVLQ
Sbjct: 121 FDLAFTSDYPNTPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPSISTVLQ 180

Query: 181 ILISIQALVLNEKPYFNEPELE--EEAEAEIYSDAYNKEAYLFCCKMMMRLMKKAPKNFE 240
           ILISIQ LVLNEKPYFNEPELE  E  + EIYSDAYNKE YL CC+MMMRLMK   KN+E
Sbjct: 181 ILISIQGLVLNEKPYFNEPELEKDEADQGEIYSDAYNKEVYLLCCEMMMRLMKNPVKNYE 240

Query: 241 EFVRNYFGEKGKVILAACKAYSDGRIRVGRFNEKENSESES---DYCNRADVSAIFKALM 300
           EFVRNYFG KG+ ILAAC+AYSDGR+RVGR+NEKEN+ + +   + C R DVSAIFK LM
Sbjct: 241 EFVRNYFGGKGERILAACEAYSDGRVRVGRYNEKENNNNNNNNGENCKRGDVSAIFKTLM 300

Query: 301 EKMHRDLHAAFLLICPS 306
           EKMHRDLHAAFL  C S
Sbjct: 301 EKMHRDLHAAFLHFCTS 317

BLAST of HG10010417 vs. NCBI nr
Match: XP_008458344.1 (PREDICTED: putative ubiquitin-conjugating enzyme E2 39 [Cucumis melo] >TYK02958.1 putative ubiquitin-conjugating enzyme E2 39 [Cucumis melo var. makuwa])

HSP 1 Score: 519.6 bits (1337), Expect = 1.8e-143
Identity = 258/320 (80.62%), Postives = 274/320 (85.62%), Query Frame = 0

Query: 3   SPPLCYSHDDKDKHPATIDGGDEPERS------PPPSFSFKRFDIVTDYSDHHYADSNLF 62
           SPPL    D +DKHP T+DGGDEP RS       PPSFSFKRFDIV+DYSDHHYADSNLF
Sbjct: 9   SPPL----DGEDKHPTTVDGGDEPNRSLSSPSPSPPSFSFKRFDIVSDYSDHHYADSNLF 68

Query: 63  ANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDL 122
            NCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGP+GTPYHDGLFFFDL
Sbjct: 69  PNCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPSGTPYHDGLFFFDL 128

Query: 123 AFTSDYPISPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILI 182
           AFTSDYPI+PPMVY HSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNP+ISTVLQILI
Sbjct: 129 AFTSDYPITPPMVYCHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPSISTVLQILI 188

Query: 183 SIQALVLNEKPYFNEPELEEEA---EAEIYSDAYNKEAYLFCCKMMMRLMKKAPKNFEEF 242
           SIQALVLNEKPYFNEPELEEE    + EIYSDAYNKE YL CC+MMMRLMK   KN+EEF
Sbjct: 189 SIQALVLNEKPYFNEPELEEEEADDQGEIYSDAYNKEVYLLCCEMMMRLMKNPVKNYEEF 248

Query: 243 VRNYFGEKGKVILAACKAYSDGRIRVGRFNEKE--------NSESESDYCNRADVSAIFK 302
           VRNYFGEKG+ ILAAC+AYSDGR+RVGR+NE E        N+ +  + C R DVSAIFK
Sbjct: 249 VRNYFGEKGERILAACEAYSDGRVRVGRYNENEKENNNINNNNNNNGETCKRGDVSAIFK 308

Query: 303 ALMEKMHRDLHAAFLLICPS 306
           ALMEK HRDLHAAFL  C S
Sbjct: 309 ALMEKTHRDLHAAFLHFCTS 324

BLAST of HG10010417 vs. NCBI nr
Match: XP_022930350.1 (probable ubiquitin-conjugating enzyme E2 25 [Cucurbita moschata])

HSP 1 Score: 491.5 bits (1264), Expect = 5.2e-135
Identity = 247/299 (82.61%), Postives = 263/299 (87.96%), Query Frame = 0

Query: 6   LCYSHDDKDKHPATIDGGDEPERSPPPSFSFKRFDIVTDYSDHHYADSNLFANCIGSDVS 65
           LCYSHD +DK P T     EPERSPP    FKRFDIVTD+SDHHYA SNLF NCIGSDVS
Sbjct: 5   LCYSHDAEDKQPPT---ALEPERSPP----FKRFDIVTDFSDHHYAHSNLFPNCIGSDVS 64

Query: 66  KKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTSDYPIS 125
           KKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAF+SDYPI 
Sbjct: 65  KKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPIC 124

Query: 126 PPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILISIQALVLNE 185
           PP+VYYHSYGLRLNPNLYEDGYVCLSLINTWDGK+EERWNPAISTVLQILISIQALVLNE
Sbjct: 125 PPIVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALVLNE 184

Query: 186 KPYFNEPELEEEAEAEIYSDAYNKEAYLFCCKMMMRLMKKAPKNFEEFVRNYFGEKGKVI 245
           +PYFNEPEL+E AEAEIYSDAYNKEAYLFCCKM MRL++  PKNFEEFVR+YF ++GK I
Sbjct: 185 RPYFNEPELDEAAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKGI 244

Query: 246 LAACKAYSDGRIRVGRF--NEKEN---SESESDYCNRADVSAIFKALMEKMHRDLHAAF 300
           LAACKAYS+GR+RVGRF  +E EN   S S S+  N   VSAIFKALMEK HRDL AAF
Sbjct: 245 LAACKAYSEGRVRVGRFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEKTHRDLQAAF 296

BLAST of HG10010417 vs. NCBI nr
Match: XP_022999956.1 (probable ubiquitin-conjugating enzyme E2 25 [Cucurbita maxima])

HSP 1 Score: 491.1 bits (1263), Expect = 6.8e-135
Identity = 247/299 (82.61%), Postives = 263/299 (87.96%), Query Frame = 0

Query: 6   LCYSHDDKDKHPATIDGGDEPERSPPPSFSFKRFDIVTDYSDHHYADSNLFANCIGSDVS 65
           LCYSH  +DK P T     EPERSPP    FKRFDIVTD+SDHHYA SNLF NCIGSDVS
Sbjct: 5   LCYSHHAEDKQPPT---PHEPERSPP----FKRFDIVTDFSDHHYAHSNLFPNCIGSDVS 64

Query: 66  KKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTSDYPIS 125
           KKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAF+SDYPIS
Sbjct: 65  KKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPIS 124

Query: 126 PPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILISIQALVLNE 185
           PP+VYYHSYGLRLNPNLYEDGYVCLSLINTWDGK+EERWNPAISTVLQILISIQALVLNE
Sbjct: 125 PPIVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALVLNE 184

Query: 186 KPYFNEPELEEEAEAEIYSDAYNKEAYLFCCKMMMRLMKKAPKNFEEFVRNYFGEKGKVI 245
           +PYFNEPEL+E AEAEIYSDAYNKEAYLFCCKM MRL++  PKNFEEFVR+YF ++GK I
Sbjct: 185 RPYFNEPELDEAAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKGI 244

Query: 246 LAACKAYSDGRIRVGRF--NEKEN---SESESDYCNRADVSAIFKALMEKMHRDLHAAF 300
           LAACKAYS+GR+RVGRF  +E EN   S S S+  N   VSAIFKALMEK HRDL AAF
Sbjct: 245 LAACKAYSEGRVRVGRFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEKTHRDLQAAF 296

BLAST of HG10010417 vs. ExPASy Swiss-Prot
Match: Q9LUQ5 (Probable ubiquitin-conjugating enzyme E2 25 OS=Arabidopsis thaliana OX=3702 GN=UBC25 PE=2 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 1.2e-68
Identity = 130/262 (49.62%), Postives = 170/262 (64.89%), Query Frame = 0

Query: 36  FKRFDIVTDYSDHHYADSNLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLL 95
           FKRFDIV D+SDHHYA     +     D +K+I  EW+ILE++LPE IFVRAYE R+DLL
Sbjct: 305 FKRFDIVEDFSDHHYASKGTTSKQHSKDWAKRIQDEWRILEKDLPEMIFVRAYESRMDLL 364

Query: 96  RAVIVGPAGTPYHDGLFFFDLAFTSDYPISPPMVYYHSYGLRLNPNLYEDGYVCLSLINT 155
           RAVI+G  GTPYHDGLFFFD+ F   YP +PP+V+YHS GLR+NPNLY  G VCLSL+ T
Sbjct: 365 RAVIIGAQGTPYHDGLFFFDIFFPDTYPSTPPIVHYHSGGLRINPNLYNCGKVCLSLLGT 424

Query: 156 WDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPELEE---EAEAEIYSDAYNKEAY 215
           W G + E+W P  ST+LQ+L+SIQ L+LN+KPYFNEP  E     A  E  S AY++  +
Sbjct: 425 WSGNQREKWIPNTSTMLQVLVSIQGLILNQKPYFNEPGYERSAGSAHGESTSKAYSENTF 484

Query: 216 LFCCKMMMRLMKKAPKNFEEFVRNYFGEKGKVILAACKAYSDGR----IRVGRFNEKENS 275
           +   K M+  M++ PK FE+F   +F      +L AC AY +G     +  G  + +ENS
Sbjct: 485 ILSLKTMVYTMRRPPKYFEDFAYGHFFSCAHDVLKACNAYRNGATPGYLVKGAPDVEENS 544

Query: 276 ESESDYCNRADVSAIFKALMEK 291
              S    R DV+   + ++ K
Sbjct: 545 AGMSSLKFRTDVATFVETVLLK 566

BLAST of HG10010417 vs. ExPASy Swiss-Prot
Match: Q9ZVX1 (Probable ubiquitin-conjugating enzyme E2 23 OS=Arabidopsis thaliana OX=3702 GN=UBC23 PE=1 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 1.3e-67
Identity = 138/287 (48.08%), Postives = 179/287 (62.37%), Query Frame = 0

Query: 20   IDGGDEPERSPPPSFSFKRFDIVTDYSDHHYADSNLFANCIGSDVS-----KKIMREWKI 79
            ++  D+P  S   S SF+RFDI  D  DHH      F    G         KK+ ++WKI
Sbjct: 808  LEDEDKPVPSEGDSCSFRRFDISQDPLDHH------FLGVDGQKTKERQWFKKVDQDWKI 867

Query: 80   LERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTSDYPISPPMVYYHSY 139
            L+ NLP+ IFVRAYE+R+DLLRAVIVG  GTPY DGLFFFD    SDYP  PP  YYHS 
Sbjct: 868  LQNNLPDGIFVRAYEDRMDLLRAVIVGAFGTPYQDGLFFFDFHLPSDYPSVPPSAYYHSG 927

Query: 140  GLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPEL 199
            G RLNPNLYE+G VCLSL+NTW G+  E W+P  S++LQ+L+S+Q LVLN KPYFNE   
Sbjct: 928  GWRLNPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGY 987

Query: 200  EEE---AEAEIYSDAYNKEAYLFCCKMMMRLMKKAPKNFEEFVRNYFGEKGKVILAACKA 259
            +++   AE E  S  YN+  +L  CK MM LM+K PK+FEE ++++F ++G  IL AC A
Sbjct: 988  DKQVGTAEGEKNSLGYNENTFLLNCKTMMYLMRKPPKDFEELIKDHFRKRGYYILKACDA 1047

Query: 260  YSDGRIRVGRFNEKENSESESDYCNRADVSAIFKALMEKMHRDLHAA 299
            Y  G + +G   +  +   E    N   V   FK ++ K+   L +A
Sbjct: 1048 YMKGYL-IGSLTKDASVIDERSSANSTSVG--FKLMLAKIAPKLFSA 1085

BLAST of HG10010417 vs. ExPASy Swiss-Prot
Match: Q8VY10 (Probable ubiquitin-conjugating enzyme E2 24 OS=Arabidopsis thaliana OX=3702 GN=UBC24 PE=1 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 3.7e-67
Identity = 131/269 (48.70%), Postives = 176/269 (65.43%), Query Frame = 0

Query: 36  FKRFDIVTDYSDHHY--ADSNLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERID 95
           F++FD+V D SDHH+  +D  L  + +     KK+ +EW  LE NLP +I+VR  EER+D
Sbjct: 633 FRQFDMVNDCSDHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVCEERMD 692

Query: 96  LLRAVIVGPAGTPYHDGLFFFDLAFTSDYPISPPMVYYHSYGLRLNPNLYEDGYVCLSLI 155
           LLRA +VG  GTPYHDGLFFFD+     YP  PPMV+YHS G+RLNPNLYE G VCLSL+
Sbjct: 693 LLRAALVGAPGTPYHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNLYESGRVCLSLL 752

Query: 156 NTWDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPELEEE---AEAEIYSDAYNKE 215
           NTW G   E WN   S++LQ+L+S QALVLNEKPYFNE   +++   AE E  S +YN+ 
Sbjct: 753 NTWSGSGTEVWNAGSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAEGEKNSVSYNEN 812

Query: 216 AYLFCCKMMMRLMKKAPKNFEEFVRNYFGEKGKVILAACKAYSDGRIRVGRFNEKENSES 275
           A+L  CK M+ +++K PK+FE  V+++F  + + +LAACKAY +G + VG         S
Sbjct: 813 AFLITCKSMISMLRKPPKHFEMLVKDHFTHRAQHVLAACKAYMEG-VPVG--------SS 872

Query: 276 ESDYCNRADVSAIFKALMEKMHRDLHAAF 300
            +   N    S  FK ++ K++  L  AF
Sbjct: 873 ANLQGNSTTNSTGFKIMLSKLYPKLLEAF 892

BLAST of HG10010417 vs. ExPASy Swiss-Prot
Match: Q9C918 (Putative ubiquitin-conjugating enzyme E2 38 OS=Arabidopsis thaliana OX=3702 GN=UBC38 PE=3 SV=2)

HSP 1 Score: 244.2 bits (622), Expect = 1.9e-63
Identity = 123/263 (46.77%), Postives = 166/263 (63.12%), Query Frame = 0

Query: 35  SFKRFDIVTDYSDHHYADSNLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDL 94
           +FKRFD V D+SDHHY      +     +  KK+  EWKIL +NLPE+IFVRA E R+DL
Sbjct: 26  NFKRFDSVEDFSDHHYVSKGKASKQHSKNWVKKVQDEWKILNQNLPETIFVRACESRMDL 85

Query: 95  LRAVIVGPAGTPYHDGLFFFDLAFTSDYPISPPMVYYHSYGLRLNPNLYEDGYVCLSLIN 154
           LRAVI+G  GTPYHDGLFFFD+ F   YP  PP VYYHS GLR+NPNLY  G VCLSL+ 
Sbjct: 86  LRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVYYHSGGLRINPNLYNCGKVCLSLLG 145

Query: 155 TWDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPE---LEEEAEAEIYSDAYNKEA 214
           TW G   + W P  ST+LQ+L+SIQALVLNE+PYFNEP    ++     E  S  Y++  
Sbjct: 146 TWHGNARQSWLPKESTMLQVLVSIQALVLNEQPYFNEPGYGLIKGTWLGESKSKVYSENV 205

Query: 215 YLFCCKMMMRLMKKAPKNFEEFVRNYFGEKGKVILAACKAYSD----GRIRVGRFNEKEN 274
           +L   K M+  M+K P++FEE+V+N++  +   I+ AC AY      G +  G   + E 
Sbjct: 206 FLLSLKTMVYSMRKPPQHFEEYVQNHYFVRSHDIVKACNAYKAGAPLGSMVKGGVQDLEQ 265

Query: 275 SESESDYCNRADVSAIFKALMEK 291
           +        + DV++  + ++++
Sbjct: 266 ARQSGSKKFKTDVASFMQTVVDE 288

BLAST of HG10010417 vs. ExPASy Swiss-Prot
Match: F4HPP7 (Putative ubiquitin-conjugating enzyme E2 39 OS=Arabidopsis thaliana OX=3702 GN=UBC39 PE=3 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 2.5e-63
Identity = 131/270 (48.52%), Postives = 171/270 (63.33%), Query Frame = 0

Query: 36  FKRFDIVTDYSDHHYADSNLFANCI---------GSDVSKKIMREWKILERNLPESIFVR 95
           FKRFD V D+SDHHYA     A              +  K I +EWKIL++NLPE+IFVR
Sbjct: 21  FKRFDTVEDFSDHHYAAEGSPAKLSLWHLKFKGRPKNWVKDIQKEWKILDKNLPETIFVR 80

Query: 96  AYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTSDYPISPPMVYYHSYGLRLNPNLYEDG 155
           A E RIDLLRAVI+G  GTPYHDGLFFFD+ F   YP  PP V+YHS GLR+NPNLY+ G
Sbjct: 81  ACESRIDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPKVHYHSGGLRINPNLYKCG 140

Query: 156 YVCLSLINTWDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPELEEEAE---AEIY 215
            VCLSLI+TW GK+ E+W P  ST+LQ+L+SIQAL+LNEKPY+NEP  E+       E Y
Sbjct: 141 KVCLSLISTWTGKKREKWLPKESTMLQLLVSIQALILNEKPYYNEPGYEKSMGTPLGESY 200

Query: 216 SDAYNKEAYLFCCKMMMRLMKKAPKNFEEFVRNYFGEKGKVILAACKAYSDGRIRVGRFN 275
           S  Y++  ++F  K M         +FEEFVR++F  +   I+ AC AY DG   VG  +
Sbjct: 201 SKDYSENVFVFSLKTM---------HFEEFVRSHFFVRSHDIVKACNAYKDG-APVGSID 260

Query: 276 E---KENSESESDYCNRADVSAIFKALMEK 291
           +   K+ +        R +V++  K ++++
Sbjct: 261 KGGVKKQTRQRGSLKFRINVTSFMKTVVDE 280

BLAST of HG10010417 vs. ExPASy TrEMBL
Match: A0A0A0KF71 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G330470 PE=4 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 5.1e-144
Identity = 258/317 (81.39%), Postives = 275/317 (86.75%), Query Frame = 0

Query: 1   MASPPLCYSH---DDKDKHPATIDGGDEPERS----PPPSFSFKRFDIVTDYSDHHYADS 60
           MASP L  S    D +DKHP T+DGGDEP RS     PPSFSFKRFDIVTDYSDHHYADS
Sbjct: 1   MASPSLPPSPPPLDGEDKHPTTVDGGDEPNRSLSSPSPPSFSFKRFDIVTDYSDHHYADS 60

Query: 61  NLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFF 120
           NLF NCIGSDVSKKIMREWK+LERNLPESIFVRAYEERIDLLRAVIVGP+GTPYHDGLFF
Sbjct: 61  NLFPNCIGSDVSKKIMREWKVLERNLPESIFVRAYEERIDLLRAVIVGPSGTPYHDGLFF 120

Query: 121 FDLAFTSDYPISPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQ 180
           FDLAFTSDYP +PPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNP+ISTVLQ
Sbjct: 121 FDLAFTSDYPNTPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPSISTVLQ 180

Query: 181 ILISIQALVLNEKPYFNEPELE--EEAEAEIYSDAYNKEAYLFCCKMMMRLMKKAPKNFE 240
           ILISIQ LVLNEKPYFNEPELE  E  + EIYSDAYNKE YL CC+MMMRLMK   KN+E
Sbjct: 181 ILISIQGLVLNEKPYFNEPELEKDEADQGEIYSDAYNKEVYLLCCEMMMRLMKNPVKNYE 240

Query: 241 EFVRNYFGEKGKVILAACKAYSDGRIRVGRFNEKENSESES---DYCNRADVSAIFKALM 300
           EFVRNYFG KG+ ILAAC+AYSDGR+RVGR+NEKEN+ + +   + C R DVSAIFK LM
Sbjct: 241 EFVRNYFGGKGERILAACEAYSDGRVRVGRYNEKENNNNNNNNGENCKRGDVSAIFKTLM 300

Query: 301 EKMHRDLHAAFLLICPS 306
           EKMHRDLHAAFL  C S
Sbjct: 301 EKMHRDLHAAFLHFCTS 317

BLAST of HG10010417 vs. ExPASy TrEMBL
Match: A0A5D3BVE0 (Putative ubiquitin-conjugating enzyme E2 39 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00410 PE=4 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 8.7e-144
Identity = 258/320 (80.62%), Postives = 274/320 (85.62%), Query Frame = 0

Query: 3   SPPLCYSHDDKDKHPATIDGGDEPERS------PPPSFSFKRFDIVTDYSDHHYADSNLF 62
           SPPL    D +DKHP T+DGGDEP RS       PPSFSFKRFDIV+DYSDHHYADSNLF
Sbjct: 9   SPPL----DGEDKHPTTVDGGDEPNRSLSSPSPSPPSFSFKRFDIVSDYSDHHYADSNLF 68

Query: 63  ANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDL 122
            NCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGP+GTPYHDGLFFFDL
Sbjct: 69  PNCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPSGTPYHDGLFFFDL 128

Query: 123 AFTSDYPISPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILI 182
           AFTSDYPI+PPMVY HSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNP+ISTVLQILI
Sbjct: 129 AFTSDYPITPPMVYCHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPSISTVLQILI 188

Query: 183 SIQALVLNEKPYFNEPELEEEA---EAEIYSDAYNKEAYLFCCKMMMRLMKKAPKNFEEF 242
           SIQALVLNEKPYFNEPELEEE    + EIYSDAYNKE YL CC+MMMRLMK   KN+EEF
Sbjct: 189 SIQALVLNEKPYFNEPELEEEEADDQGEIYSDAYNKEVYLLCCEMMMRLMKNPVKNYEEF 248

Query: 243 VRNYFGEKGKVILAACKAYSDGRIRVGRFNEKE--------NSESESDYCNRADVSAIFK 302
           VRNYFGEKG+ ILAAC+AYSDGR+RVGR+NE E        N+ +  + C R DVSAIFK
Sbjct: 249 VRNYFGEKGERILAACEAYSDGRVRVGRYNENEKENNNINNNNNNNGETCKRGDVSAIFK 308

Query: 303 ALMEKMHRDLHAAFLLICPS 306
           ALMEK HRDLHAAFL  C S
Sbjct: 309 ALMEKTHRDLHAAFLHFCTS 324

BLAST of HG10010417 vs. ExPASy TrEMBL
Match: A0A1S3C8W9 (putative ubiquitin-conjugating enzyme E2 39 OS=Cucumis melo OX=3656 GN=LOC103497784 PE=4 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 8.7e-144
Identity = 258/320 (80.62%), Postives = 274/320 (85.62%), Query Frame = 0

Query: 3   SPPLCYSHDDKDKHPATIDGGDEPERS------PPPSFSFKRFDIVTDYSDHHYADSNLF 62
           SPPL    D +DKHP T+DGGDEP RS       PPSFSFKRFDIV+DYSDHHYADSNLF
Sbjct: 9   SPPL----DGEDKHPTTVDGGDEPNRSLSSPSPSPPSFSFKRFDIVSDYSDHHYADSNLF 68

Query: 63  ANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDL 122
            NCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGP+GTPYHDGLFFFDL
Sbjct: 69  PNCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPSGTPYHDGLFFFDL 128

Query: 123 AFTSDYPISPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILI 182
           AFTSDYPI+PPMVY HSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNP+ISTVLQILI
Sbjct: 129 AFTSDYPITPPMVYCHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPSISTVLQILI 188

Query: 183 SIQALVLNEKPYFNEPELEEEA---EAEIYSDAYNKEAYLFCCKMMMRLMKKAPKNFEEF 242
           SIQALVLNEKPYFNEPELEEE    + EIYSDAYNKE YL CC+MMMRLMK   KN+EEF
Sbjct: 189 SIQALVLNEKPYFNEPELEEEEADDQGEIYSDAYNKEVYLLCCEMMMRLMKNPVKNYEEF 248

Query: 243 VRNYFGEKGKVILAACKAYSDGRIRVGRFNEKE--------NSESESDYCNRADVSAIFK 302
           VRNYFGEKG+ ILAAC+AYSDGR+RVGR+NE E        N+ +  + C R DVSAIFK
Sbjct: 249 VRNYFGEKGERILAACEAYSDGRVRVGRYNENEKENNNINNNNNNNGETCKRGDVSAIFK 308

Query: 303 ALMEKMHRDLHAAFLLICPS 306
           ALMEK HRDLHAAFL  C S
Sbjct: 309 ALMEKTHRDLHAAFLHFCTS 324

BLAST of HG10010417 vs. ExPASy TrEMBL
Match: A0A6J1EWP5 (probable ubiquitin-conjugating enzyme E2 25 OS=Cucurbita moschata OX=3662 GN=LOC111436820 PE=4 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 2.5e-135
Identity = 247/299 (82.61%), Postives = 263/299 (87.96%), Query Frame = 0

Query: 6   LCYSHDDKDKHPATIDGGDEPERSPPPSFSFKRFDIVTDYSDHHYADSNLFANCIGSDVS 65
           LCYSHD +DK P T     EPERSPP    FKRFDIVTD+SDHHYA SNLF NCIGSDVS
Sbjct: 5   LCYSHDAEDKQPPT---ALEPERSPP----FKRFDIVTDFSDHHYAHSNLFPNCIGSDVS 64

Query: 66  KKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTSDYPIS 125
           KKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAF+SDYPI 
Sbjct: 65  KKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPIC 124

Query: 126 PPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILISIQALVLNE 185
           PP+VYYHSYGLRLNPNLYEDGYVCLSLINTWDGK+EERWNPAISTVLQILISIQALVLNE
Sbjct: 125 PPIVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALVLNE 184

Query: 186 KPYFNEPELEEEAEAEIYSDAYNKEAYLFCCKMMMRLMKKAPKNFEEFVRNYFGEKGKVI 245
           +PYFNEPEL+E AEAEIYSDAYNKEAYLFCCKM MRL++  PKNFEEFVR+YF ++GK I
Sbjct: 185 RPYFNEPELDEAAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKGI 244

Query: 246 LAACKAYSDGRIRVGRF--NEKEN---SESESDYCNRADVSAIFKALMEKMHRDLHAAF 300
           LAACKAYS+GR+RVGRF  +E EN   S S S+  N   VSAIFKALMEK HRDL AAF
Sbjct: 245 LAACKAYSEGRVRVGRFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEKTHRDLQAAF 296

BLAST of HG10010417 vs. ExPASy TrEMBL
Match: A0A6J1KEJ5 (probable ubiquitin-conjugating enzyme E2 25 OS=Cucurbita maxima OX=3661 GN=LOC111494283 PE=4 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 3.3e-135
Identity = 247/299 (82.61%), Postives = 263/299 (87.96%), Query Frame = 0

Query: 6   LCYSHDDKDKHPATIDGGDEPERSPPPSFSFKRFDIVTDYSDHHYADSNLFANCIGSDVS 65
           LCYSH  +DK P T     EPERSPP    FKRFDIVTD+SDHHYA SNLF NCIGSDVS
Sbjct: 5   LCYSHHAEDKQPPT---PHEPERSPP----FKRFDIVTDFSDHHYAHSNLFPNCIGSDVS 64

Query: 66  KKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTSDYPIS 125
           KKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAF+SDYPIS
Sbjct: 65  KKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPIS 124

Query: 126 PPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILISIQALVLNE 185
           PP+VYYHSYGLRLNPNLYEDGYVCLSLINTWDGK+EERWNPAISTVLQILISIQALVLNE
Sbjct: 125 PPIVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALVLNE 184

Query: 186 KPYFNEPELEEEAEAEIYSDAYNKEAYLFCCKMMMRLMKKAPKNFEEFVRNYFGEKGKVI 245
           +PYFNEPEL+E AEAEIYSDAYNKEAYLFCCKM MRL++  PKNFEEFVR+YF ++GK I
Sbjct: 185 RPYFNEPELDEAAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKGI 244

Query: 246 LAACKAYSDGRIRVGRF--NEKEN---SESESDYCNRADVSAIFKALMEKMHRDLHAAF 300
           LAACKAYS+GR+RVGRF  +E EN   S S S+  N   VSAIFKALMEK HRDL AAF
Sbjct: 245 LAACKAYSEGRVRVGRFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEKTHRDLQAAF 296

BLAST of HG10010417 vs. TAIR 10
Match: AT3G15355.1 (ubiquitin-conjugating enzyme 25 )

HSP 1 Score: 261.5 bits (667), Expect = 8.2e-70
Identity = 130/262 (49.62%), Postives = 170/262 (64.89%), Query Frame = 0

Query: 36  FKRFDIVTDYSDHHYADSNLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLL 95
           FKRFDIV D+SDHHYA     +     D +K+I  EW+ILE++LPE IFVRAYE R+DLL
Sbjct: 305 FKRFDIVEDFSDHHYASKGTTSKQHSKDWAKRIQDEWRILEKDLPEMIFVRAYESRMDLL 364

Query: 96  RAVIVGPAGTPYHDGLFFFDLAFTSDYPISPPMVYYHSYGLRLNPNLYEDGYVCLSLINT 155
           RAVI+G  GTPYHDGLFFFD+ F   YP +PP+V+YHS GLR+NPNLY  G VCLSL+ T
Sbjct: 365 RAVIIGAQGTPYHDGLFFFDIFFPDTYPSTPPIVHYHSGGLRINPNLYNCGKVCLSLLGT 424

Query: 156 WDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPELEE---EAEAEIYSDAYNKEAY 215
           W G + E+W P  ST+LQ+L+SIQ L+LN+KPYFNEP  E     A  E  S AY++  +
Sbjct: 425 WSGNQREKWIPNTSTMLQVLVSIQGLILNQKPYFNEPGYERSAGSAHGESTSKAYSENTF 484

Query: 216 LFCCKMMMRLMKKAPKNFEEFVRNYFGEKGKVILAACKAYSDGR----IRVGRFNEKENS 275
           +   K M+  M++ PK FE+F   +F      +L AC AY +G     +  G  + +ENS
Sbjct: 485 ILSLKTMVYTMRRPPKYFEDFAYGHFFSCAHDVLKACNAYRNGATPGYLVKGAPDVEENS 544

Query: 276 ESESDYCNRADVSAIFKALMEK 291
              S    R DV+   + ++ K
Sbjct: 545 AGMSSLKFRTDVATFVETVLLK 566

BLAST of HG10010417 vs. TAIR 10
Match: AT2G16920.1 (ubiquitin-conjugating enzyme 23 )

HSP 1 Score: 258.1 bits (658), Expect = 9.1e-69
Identity = 138/287 (48.08%), Postives = 179/287 (62.37%), Query Frame = 0

Query: 20   IDGGDEPERSPPPSFSFKRFDIVTDYSDHHYADSNLFANCIGSDVS-----KKIMREWKI 79
            ++  D+P  S   S SF+RFDI  D  DHH      F    G         KK+ ++WKI
Sbjct: 808  LEDEDKPVPSEGDSCSFRRFDISQDPLDHH------FLGVDGQKTKERQWFKKVDQDWKI 867

Query: 80   LERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTSDYPISPPMVYYHSY 139
            L+ NLP+ IFVRAYE+R+DLLRAVIVG  GTPY DGLFFFD    SDYP  PP  YYHS 
Sbjct: 868  LQNNLPDGIFVRAYEDRMDLLRAVIVGAFGTPYQDGLFFFDFHLPSDYPSVPPSAYYHSG 927

Query: 140  GLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPEL 199
            G RLNPNLYE+G VCLSL+NTW G+  E W+P  S++LQ+L+S+Q LVLN KPYFNE   
Sbjct: 928  GWRLNPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGY 987

Query: 200  EEE---AEAEIYSDAYNKEAYLFCCKMMMRLMKKAPKNFEEFVRNYFGEKGKVILAACKA 259
            +++   AE E  S  YN+  +L  CK MM LM+K PK+FEE ++++F ++G  IL AC A
Sbjct: 988  DKQVGTAEGEKNSLGYNENTFLLNCKTMMYLMRKPPKDFEELIKDHFRKRGYYILKACDA 1047

Query: 260  YSDGRIRVGRFNEKENSESESDYCNRADVSAIFKALMEKMHRDLHAA 299
            Y  G + +G   +  +   E    N   V   FK ++ K+   L +A
Sbjct: 1048 YMKGYL-IGSLTKDASVIDERSSANSTSVG--FKLMLAKIAPKLFSA 1085

BLAST of HG10010417 vs. TAIR 10
Match: AT2G33770.1 (phosphate 2 )

HSP 1 Score: 256.5 bits (654), Expect = 2.6e-68
Identity = 131/269 (48.70%), Postives = 176/269 (65.43%), Query Frame = 0

Query: 36  FKRFDIVTDYSDHHY--ADSNLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERID 95
           F++FD+V D SDHH+  +D  L  + +     KK+ +EW  LE NLP +I+VR  EER+D
Sbjct: 633 FRQFDMVNDCSDHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVCEERMD 692

Query: 96  LLRAVIVGPAGTPYHDGLFFFDLAFTSDYPISPPMVYYHSYGLRLNPNLYEDGYVCLSLI 155
           LLRA +VG  GTPYHDGLFFFD+     YP  PPMV+YHS G+RLNPNLYE G VCLSL+
Sbjct: 693 LLRAALVGAPGTPYHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNLYESGRVCLSLL 752

Query: 156 NTWDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPELEEE---AEAEIYSDAYNKE 215
           NTW G   E WN   S++LQ+L+S QALVLNEKPYFNE   +++   AE E  S +YN+ 
Sbjct: 753 NTWSGSGTEVWNAGSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAEGEKNSVSYNEN 812

Query: 216 AYLFCCKMMMRLMKKAPKNFEEFVRNYFGEKGKVILAACKAYSDGRIRVGRFNEKENSES 275
           A+L  CK M+ +++K PK+FE  V+++F  + + +LAACKAY +G + VG         S
Sbjct: 813 AFLITCKSMISMLRKPPKHFEMLVKDHFTHRAQHVLAACKAYMEG-VPVG--------SS 872

Query: 276 ESDYCNRADVSAIFKALMEKMHRDLHAAF 300
            +   N    S  FK ++ K++  L  AF
Sbjct: 873 ANLQGNSTTNSTGFKIMLSKLYPKLLEAF 892

BLAST of HG10010417 vs. TAIR 10
Match: AT1G53023.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 243.8 bits (621), Expect = 1.8e-64
Identity = 131/270 (48.52%), Postives = 171/270 (63.33%), Query Frame = 0

Query: 36  FKRFDIVTDYSDHHYADSNLFANCI---------GSDVSKKIMREWKILERNLPESIFVR 95
           FKRFD V D+SDHHYA     A              +  K I +EWKIL++NLPE+IFVR
Sbjct: 21  FKRFDTVEDFSDHHYAAEGSPAKLSLWHLKFKGRPKNWVKDIQKEWKILDKNLPETIFVR 80

Query: 96  AYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTSDYPISPPMVYYHSYGLRLNPNLYEDG 155
           A E RIDLLRAVI+G  GTPYHDGLFFFD+ F   YP  PP V+YHS GLR+NPNLY+ G
Sbjct: 81  ACESRIDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPKVHYHSGGLRINPNLYKCG 140

Query: 156 YVCLSLINTWDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPELEEEAE---AEIY 215
            VCLSLI+TW GK+ E+W P  ST+LQ+L+SIQAL+LNEKPY+NEP  E+       E Y
Sbjct: 141 KVCLSLISTWTGKKREKWLPKESTMLQLLVSIQALILNEKPYYNEPGYEKSMGTPLGESY 200

Query: 216 SDAYNKEAYLFCCKMMMRLMKKAPKNFEEFVRNYFGEKGKVILAACKAYSDGRIRVGRFN 275
           S  Y++  ++F  K M         +FEEFVR++F  +   I+ AC AY DG   VG  +
Sbjct: 201 SKDYSENVFVFSLKTM---------HFEEFVRSHFFVRSHDIVKACNAYKDG-APVGSID 260

Query: 276 E---KENSESESDYCNRADVSAIFKALMEK 291
           +   K+ +        R +V++  K ++++
Sbjct: 261 KGGVKKQTRQRGSLKFRINVTSFMKTVVDE 280

BLAST of HG10010417 vs. TAIR 10
Match: AT1G53025.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 242.7 bits (618), Expect = 4.0e-64
Identity = 121/262 (46.18%), Postives = 166/262 (63.36%), Query Frame = 0

Query: 36  FKRFDIVTDYSDHHYADSNLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLL 95
           FKRFD V D+SDHHYA     +     +  KK+  +WKILE +LPE+I VRA E R+DLL
Sbjct: 244 FKRFDTVEDFSDHHYASKGKSSKQHSKNWVKKVQADWKILENDLPEAISVRACESRMDLL 303

Query: 96  RAVIVGPAGTPYHDGLFFFDLAFTSDYPISPPMVYYHSYGLRLNPNLYEDGYVCLSLINT 155
           RAVI+G  GTPYHDGLFFFD+ F   YP  PP V+YHS GLR+NPNLY  G VCLSL+ T
Sbjct: 304 RAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVHYHSGGLRINPNLYNCGKVCLSLLGT 363

Query: 156 WDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPELEEE---AEAEIYSDAYNKEAY 215
           W G   E+W P  ST+LQ+L+SIQAL+LNEKPYFNEP   +    A  E  S  Y++  +
Sbjct: 364 WAGSAREKWLPNESTMLQLLVSIQALILNEKPYFNEPGYVQSAGTASGESKSKVYSENVF 423

Query: 216 LFCCKMMMRLMKKAPKNFEEFVRNYFGEKGKVILAACKAYSD----GRIRVGRFNEKENS 275
           L   K M+  +++ P++FEE+V+N++  +   I+ AC AY      G +  G   + E +
Sbjct: 424 LLSLKTMVYSIRRPPQHFEEYVQNHYFVRSHDIVKACNAYKAGAPLGSMVKGGVQDLEEA 483

Query: 276 ESESDYCNRADVSAIFKALMEK 291
                   + DV++  + ++++
Sbjct: 484 RQSGSKKFKTDVASFMQTVVDE 505

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038907021.11.3e-16292.74probable ubiquitin-conjugating enzyme E2 25 [Benincasa hispida][more]
XP_004148800.11.1e-14381.39probable ubiquitin-conjugating enzyme E2 25 [Cucumis sativus] >KGN47459.1 hypoth... [more]
XP_008458344.11.8e-14380.63PREDICTED: putative ubiquitin-conjugating enzyme E2 39 [Cucumis melo] >TYK02958.... [more]
XP_022930350.15.2e-13582.61probable ubiquitin-conjugating enzyme E2 25 [Cucurbita moschata][more]
XP_022999956.16.8e-13582.61probable ubiquitin-conjugating enzyme E2 25 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LUQ51.2e-6849.62Probable ubiquitin-conjugating enzyme E2 25 OS=Arabidopsis thaliana OX=3702 GN=U... [more]
Q9ZVX11.3e-6748.08Probable ubiquitin-conjugating enzyme E2 23 OS=Arabidopsis thaliana OX=3702 GN=U... [more]
Q8VY103.7e-6748.70Probable ubiquitin-conjugating enzyme E2 24 OS=Arabidopsis thaliana OX=3702 GN=U... [more]
Q9C9181.9e-6346.77Putative ubiquitin-conjugating enzyme E2 38 OS=Arabidopsis thaliana OX=3702 GN=U... [more]
F4HPP72.5e-6348.52Putative ubiquitin-conjugating enzyme E2 39 OS=Arabidopsis thaliana OX=3702 GN=U... [more]
Match NameE-valueIdentityDescription
A0A0A0KF715.1e-14481.39UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G330470 PE... [more]
A0A5D3BVE08.7e-14480.63Putative ubiquitin-conjugating enzyme E2 39 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3C8W98.7e-14480.63putative ubiquitin-conjugating enzyme E2 39 OS=Cucumis melo OX=3656 GN=LOC103497... [more]
A0A6J1EWP52.5e-13582.61probable ubiquitin-conjugating enzyme E2 25 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1KEJ53.3e-13582.61probable ubiquitin-conjugating enzyme E2 25 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT3G15355.18.2e-7049.62ubiquitin-conjugating enzyme 25 [more]
AT2G16920.19.1e-6948.08ubiquitin-conjugating enzyme 23 [more]
AT2G33770.12.6e-6848.70phosphate 2 [more]
AT1G53023.11.8e-6448.52Ubiquitin-conjugating enzyme family protein [more]
AT1G53025.14.0e-6446.18Ubiquitin-conjugating enzyme family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 66..220
e-value: 5.4E-25
score: 99.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 8..23
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..33
NoneNo IPR availablePANTHERPTHR46116(E3-INDEPENDENT) E2 UBIQUITIN-CONJUGATING ENZYMEcoord: 35..299
NoneNo IPR availablePANTHERPTHR46116:SF19UBIQUITIN-CONJUGATING ENZYME E2 38-RELATEDcoord: 35..299
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 68..205
e-value: 8.3E-25
score: 87.1
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 63..220
score: 37.942875
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 65..234
e-value: 1.07413E-38
score: 131.556
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 31..297
e-value: 1.0E-78
score: 266.6
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 52..224

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10010417.1HG10010417.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0061631 ubiquitin conjugating enzyme activity