HG10010415 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10010415
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	enoyl-CoA hydratase/isomerase A
Location	Chr06: 21994244 .. 21996097 (+)
RNA-Seq Expression	HG10010415
Synteny	HG10010415

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCGTTCTTCACCGGAAAACCTCATTCTCGTCAACAGAGAATCAAACGGGATCGCATTCGTTACAATCAACCGCCCGAAGTCGTTGAATTCGTTAACGAAGCCGATGATGACAGATTTGGCTCAGGCGTTTAAGAGCTTAGACCGCGACGATTCCGTGCGAGTCATTGTCCTGTCTGGATCAGGACGAGCCTTCTGTTCCGGTGTAGATCTTACTGCGGCGGAGGACGTTTTCAAGGGAGATGTGAAGGATGTTGAATCTGATCCGGTTGCGCAAATGGAACGCTGCCGGAAGCCGATCATCGGAGCGATTTCAGGTTTCGCCATCACCGCTGGATTTGAGATGGCCTTGGCTTGTGATATACTCATCGCTGCCAAAGGAGCCAAGTTCATCGACACGCATGCTAGGTTTTTGTTTTTTCTTATACTAATTTCTGAAATGAAAATTGTTGAAATTACTAAAATCTATTGGCGATGCGATTCTGTTCTGATTTCTGATATTGAAATTTCTGGAATAAAAAAAAATTGTTGTGGCAGATTTGGGATATTTCCTTCATGGGGATTATCTCAGAAACTAGGAAGGATAATTGGAGTTAACAAAGCTCGTGAAGTATCGCTAACGGCGATGCCTCTAACAGCAGAGGAAGCAGAGAGGAGGGGATTGGTGAATCAGGTGGTTGAAGGAGATGAATTGTTGAAGAAAGCTCGAGAGGTTGCGGAGGCAATCTTGAAGAACAATCAGGATTTAGTATTGAGATACAAGTCAGTCATTAACGATGGGCTGAAGCTTGATCTTGGCCAAGCTCTTGCGCTGGAAAAGGTAATCATAGTCATTCCGAAACTCTGTTCCATCATTGAATCCTGATCTTTCTGGGAAATTAATCATGGTCCCCTGAATTCCGACCAGAATTTGGCCGGGATTCATTAATTTCAATACGGATTTGGACTGAAAATTGGGAATTCGAACCACATAACTCGTGGTTAGTAGTACACTCGTATGTTAATTAGGTTATGCTCGCTTTCGTGGTTTAGATTGACTTTGTAAGTGTTTGTAAACATTTTTTTCACTTATAAATACTTATTCAAACACTTAAAAAGTGAATCCAAATATACTCGAAGTATATGAATGTGGTCGTTACTGAACAGTGGAGATTATTAACACTTGAGTGTAAAATAACCTCAAATCATAGACTAAAATCACATGTTTTTAGTCCTTCAAACCTAATTTTGATTGAAAATTGCATTTAAAAGTATAAAATTGAAAATTAAATTAATTTTAAGTGATTAAAGTGTGTCTTTAAGTTATTTTAAAAATGAAAAAAGTGATTTTAACGATTTTAAAATCTAAAATCATTCTCAAATATGCACTTAGTTTGCTATGTTGCTTTAGATTCATAAATGGTTTTTTTTTTTTTAACTCATTTTAATAATCATGTAGTAGCTAATTACAAGTTGAATTTTTAAAATTAGTTAAAAGGGTCGAATTGAATTTTACCAGTTTTGGGAGTCCAATTTTAACCATGCTACAAGAGTGAGGATTCAAATTTTATCATTAAATGTTTGGGGTCAATTTTTACAACTACGTAAATTTCTGGGTCCAATTTTAACCTCTGACAAGAGCTCAATTTTTCTCTCAAAGCCTATTAGTGATGTCCTTGATTGGAATTTATGAGTGCTAGATTTTGATATATTTATAATTCTTTAAAAATATTGTTATACACTTAATTATTAGTTTGTTTTGGGAATGCAGGAGAGAGCTCATGTGTATTACAATGGAATGACGAAAGAGCAATTCCAGAAAATGCAAGAATTTATAGCTGGTCGGAGCTCCAAGAAACCTTCCAAGTTATAG

mRNA sequence

ATGGATCGTTCTTCACCGGAAAACCTCATTCTCGTCAACAGAGAATCAAACGGGATCGCATTCGTTACAATCAACCGCCCGAAGTCGTTGAATTCGTTAACGAAGCCGATGATGACAGATTTGGCTCAGGCGTTTAAGAGCTTAGACCGCGACGATTCCGTGCGAGTCATTGTCCTGTCTGGATCAGGACGAGCCTTCTGTTCCGGTGTAGATCTTACTGCGGCGGAGGACGTTTTCAAGGGAGATGTGAAGGATGTTGAATCTGATCCGGTTGCGCAAATGGAACGCTGCCGGAAGCCGATCATCGGAGCGATTTCAGGTTTCGCCATCACCGCTGGATTTGAGATGGCCTTGGCTTGTGATATACTCATCGCTGCCAAAGGAGCCAAGTTCATCGACACGCATGCTAGGTTTTTGTTTTTTCTTATACTAATTTCTGAAATGAAAATTGTTGAAATTACTAAAATCTATTGGCGATGCGATTCTGTTCTGATTTCTGATATTGAAATTTCTGGAATAAAAAAAAATTGTTGTGGCAGATTTGGGATATTTCCTTCATGGGGATTATCTCAGAAACTAGGAAGGATAATTGGAGTTAACAAAGCTCGTGAAGTATCGCTAACGGCGATGCCTCTAACAGCAGAGGAAGCAGAGAGGAGGGGATTGGTGAATCAGGTGGTTGAAGGAGATGAATTGTTGAAGAAAGCTCGAGAGGTTGCGGAGGCAATCTTGAAGAACAATCAGGATTTAGTATTGAGATACAAGTCAGTCATTAACGATGGGCTGAAGCTTGATCTTGGCCAAGCTCTTGCGCTGGAAAAGGAGAGAGCTCATGTGTATTACAATGGAATGACGAAAGAGCAATTCCAGAAAATGCAAGAATTTATAGCTGGTCGGAGCTCCAAGAAACCTTCCAAGTTATAG

Coding sequence (CDS)

ATGGATCGTTCTTCACCGGAAAACCTCATTCTCGTCAACAGAGAATCAAACGGGATCGCATTCGTTACAATCAACCGCCCGAAGTCGTTGAATTCGTTAACGAAGCCGATGATGACAGATTTGGCTCAGGCGTTTAAGAGCTTAGACCGCGACGATTCCGTGCGAGTCATTGTCCTGTCTGGATCAGGACGAGCCTTCTGTTCCGGTGTAGATCTTACTGCGGCGGAGGACGTTTTCAAGGGAGATGTGAAGGATGTTGAATCTGATCCGGTTGCGCAAATGGAACGCTGCCGGAAGCCGATCATCGGAGCGATTTCAGGTTTCGCCATCACCGCTGGATTTGAGATGGCCTTGGCTTGTGATATACTCATCGCTGCCAAAGGAGCCAAGTTCATCGACACGCATGCTAGGTTTTTGTTTTTTCTTATACTAATTTCTGAAATGAAAATTGTTGAAATTACTAAAATCTATTGGCGATGCGATTCTGTTCTGATTTCTGATATTGAAATTTCTGGAATAAAAAAAAATTGTTGTGGCAGATTTGGGATATTTCCTTCATGGGGATTATCTCAGAAACTAGGAAGGATAATTGGAGTTAACAAAGCTCGTGAAGTATCGCTAACGGCGATGCCTCTAACAGCAGAGGAAGCAGAGAGGAGGGGATTGGTGAATCAGGTGGTTGAAGGAGATGAATTGTTGAAGAAAGCTCGAGAGGTTGCGGAGGCAATCTTGAAGAACAATCAGGATTTAGTATTGAGATACAAGTCAGTCATTAACGATGGGCTGAAGCTTGATCTTGGCCAAGCTCTTGCGCTGGAAAAGGAGAGAGCTCATGTGTATTACAATGGAATGACGAAAGAGCAATTCCAGAAAATGCAAGAATTTATAGCTGGTCGGAGCTCCAAGAAACCTTCCAAGTTATAG

Protein sequence

MDRSSPENLILVNRESNGIAFVTINRPKSLNSLTKPMMTDLAQAFKSLDRDDSVRVIVLSGSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMERCRKPIIGAISGFAITAGFEMALACDILIAAKGAKFIDTHARFLFFLILISEMKIVEITKIYWRCDSVLISDIEISGIKKNCCGRFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAERRGLVNQVVEGDELLKKAREVAEAILKNNQDLVLRYKSVINDGLKLDLGQALALEKERAHVYYNGMTKEQFQKMQEFIAGRSSKKPSKL

Homology

[Close]

BLAST of HG10010415 vs. NCBI nr
Match: XP_008458352.1 (PREDICTED: probable enoyl-CoA hydratase 1, peroxisomal [Cucumis melo])

HSP 1 Score: 469.5 bits (1207), Expect = 2.1e-128
Identity = 252/307 (82.08%), Postives = 260/307 (84.69%), Query Frame = 0


Query: 1   MDRSSPENLILVNRESNGIAFVTINRPKSLNSLTKPMMTDLAQAFKSLDRDDSVRVIVLS 60
           MDRSSPENLILVNRESNGIAF+TINRPKSLNSLT+PMM DLA+AFKSLDRDDSVRVI+LS
Sbjct: 1   MDRSSPENLILVNRESNGIAFITINRPKSLNSLTRPMMIDLARAFKSLDRDDSVRVIILS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMERCRKPIIGAISGFAITAGFEMALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQME CRKPIIGAI+GFAITAGFE+ALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFLFFLILISEMKIVEITKIYWRCDSVLISDIEISGIKKNCCGR 180
           DILIAAKGAKFIDTHA                                           R
Sbjct: 121 DILIAAKGAKFIDTHA-------------------------------------------R 180

Query: 181 FGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAERRGLVNQVVEGDELLKKAREVA 240
           FGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAE+RGLVNQVVEG ELLKKAREVA
Sbjct: 181 FGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLVNQVVEGSELLKKAREVA 240

Query: 241 EAILKNNQDLVLRYKSVINDGLKLDLGQALALEKERAHVYYNGMTKEQFQKMQEFIAGRS 300
           EAILKNNQDLVLRYKSVINDGLKLDLGQALALEKERAH YYNGMTKEQFQKMQEFIAGRS
Sbjct: 241 EAILKNNQDLVLRYKSVINDGLKLDLGQALALEKERAHAYYNGMTKEQFQKMQEFIAGRS 264

Query: 301 SKKPSKL 308
           SK+PSKL
Sbjct: 301 SKRPSKL 264

[Close]

BLAST of HG10010415 vs. NCBI nr
Match: XP_038876166.1 (probable enoyl-CoA hydratase 1, peroxisomal [Benincasa hispida])

HSP 1 Score: 461.5 bits (1186), Expect = 5.8e-126
Identity = 249/307 (81.11%), Postives = 256/307 (83.39%), Query Frame = 0


Query: 1   MDRSSPENLILVNRESNGIAFVTINRPKSLNSLTKPMMTDLAQAFKSLDRDDSVRVIVLS 60
           MDRSSPENLILV RESNGIAFVTINRPKSLNSLT+ MMTDLAQAFKSLDRDDSVRVIVLS
Sbjct: 1   MDRSSPENLILVTRESNGIAFVTINRPKSLNSLTRSMMTDLAQAFKSLDRDDSVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMERCRKPIIGAISGFAITAGFEMALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVESDPV QMERCRKPIIGAI+GFAITAGFE+ALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVVQMERCRKPIIGAIAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFLFFLILISEMKIVEITKIYWRCDSVLISDIEISGIKKNCCGR 180
           DILIAAKGA FIDTHA                                           R
Sbjct: 121 DILIAAKGAMFIDTHA-------------------------------------------R 180

Query: 181 FGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAERRGLVNQVVEGDELLKKAREVA 240
           FGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAE+R LVN+VVEG ELLKKAR +A
Sbjct: 181 FGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRNLVNEVVEGGELLKKARAIA 240

Query: 241 EAILKNNQDLVLRYKSVINDGLKLDLGQALALEKERAHVYYNGMTKEQFQKMQEFIAGRS 300
           EAILKNNQD+VLRYKSVINDGLKLDLGQALALEKERAH YYNGMTKEQFQKMQEFIAGRS
Sbjct: 241 EAILKNNQDMVLRYKSVINDGLKLDLGQALALEKERAHAYYNGMTKEQFQKMQEFIAGRS 264

Query: 301 SKKPSKL 308
           SKKPSKL
Sbjct: 301 SKKPSKL 264

[Close]

BLAST of HG10010415 vs. NCBI nr
Match: XP_004148801.1 (probable enoyl-CoA hydratase 1, peroxisomal [Cucumis sativus] >KGN47460.1 hypothetical protein Csa_021779 [Cucumis sativus])

HSP 1 Score: 461.5 bits (1186), Expect = 5.8e-126
Identity = 249/307 (81.11%), Postives = 256/307 (83.39%), Query Frame = 0


Query: 1   MDRSSPENLILVNRESNGIAFVTINRPKSLNSLTKPMMTDLAQAFKSLDRDDSVRVIVLS 60
           MDRSS ENLILVNRESNGIAF+TINRPKSLNSLTK MM DLA AFKSLDRDDSVRVI+LS
Sbjct: 1   MDRSSSENLILVNRESNGIAFITINRPKSLNSLTKHMMADLAHAFKSLDRDDSVRVIILS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMERCRKPIIGAISGFAITAGFEMALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQME CRKPIIGAI+GFAITAGFE+ALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFLFFLILISEMKIVEITKIYWRCDSVLISDIEISGIKKNCCGR 180
           DILIAAKGAKFIDTHA                                           R
Sbjct: 121 DILIAAKGAKFIDTHA-------------------------------------------R 180

Query: 181 FGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAERRGLVNQVVEGDELLKKAREVA 240
           FGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAE+RGLVNQVVEG ELLKKAREVA
Sbjct: 181 FGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLVNQVVEGSELLKKAREVA 240

Query: 241 EAILKNNQDLVLRYKSVINDGLKLDLGQALALEKERAHVYYNGMTKEQFQKMQEFIAGRS 300
           EAILKNNQDLV+RYKSVINDGLKLDLGQAL LEKERAH YYNGMTKEQFQKMQEFIAGRS
Sbjct: 241 EAILKNNQDLVVRYKSVINDGLKLDLGQALTLEKERAHAYYNGMTKEQFQKMQEFIAGRS 264

Query: 301 SKKPSKL 308
           SK+PSKL
Sbjct: 301 SKRPSKL 264

[Close]

BLAST of HG10010415 vs. NCBI nr
Match: XP_022959404.1 (probable enoyl-CoA hydratase 1, peroxisomal [Cucurbita moschata])

HSP 1 Score: 450.7 bits (1158), Expect = 1.0e-122
Identity = 241/307 (78.50%), Postives = 253/307 (82.41%), Query Frame = 0


Query: 1   MDRSSPENLILVNRESNGIAFVTINRPKSLNSLTKPMMTDLAQAFKSLDRDDSVRVIVLS 60
           MDRSSPE LILVNRE NGIAFVTINRPKSLNSLT+ MM DLAQAFK+LDRD+SVRVIVLS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKTLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMERCRKPIIGAISGFAITAGFEMALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVE+DPV QMERCRKPIIGA++GFA+TAGFE+ALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAVTAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFLFFLILISEMKIVEITKIYWRCDSVLISDIEISGIKKNCCGR 180
           DILIAAKGAKFIDTHA                                           R
Sbjct: 121 DILIAAKGAKFIDTHA-------------------------------------------R 180

Query: 181 FGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAERRGLVNQVVEGDELLKKAREVA 240
           FGIFPSWGLSQKL RIIGVNKAREVSLTAMPLTAEEAER+GLVN VV+G ELLKK RE+A
Sbjct: 181 FGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLVNHVVDGGELLKKTREIA 240

Query: 241 EAILKNNQDLVLRYKSVINDGLKLDLGQALALEKERAHVYYNGMTKEQFQKMQEFIAGRS 300
           EAILKNNQDLVLRYKSVINDGLKLDLG ALALEKERAH YYNGMTKEQF+KMQEFIAGRS
Sbjct: 241 EAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNGMTKEQFKKMQEFIAGRS 264

Query: 301 SKKPSKL 308
           SKKPSKL
Sbjct: 301 SKKPSKL 264

[Close]

BLAST of HG10010415 vs. NCBI nr
Match: KAG6575117.1 (putative enoyl-CoA hydratase 1, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 448.7 bits (1153), Expect = 3.9e-122
Identity = 241/307 (78.50%), Postives = 252/307 (82.08%), Query Frame = 0


Query: 1   MDRSSPENLILVNRESNGIAFVTINRPKSLNSLTKPMMTDLAQAFKSLDRDDSVRVIVLS 60
           MDRSSPE LILVNRE NGIAFVTINRPKSLNSLT+ MM DLAQAFK+LDRD+SVRVIVLS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKTLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMERCRKPIIGAISGFAITAGFEMALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVE+DPV QMERCRKPIIGA++GFAITAGFE+ALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFLFFLILISEMKIVEITKIYWRCDSVLISDIEISGIKKNCCGR 180
           DILIAAKGAKFIDTHA                                           R
Sbjct: 121 DILIAAKGAKFIDTHA-------------------------------------------R 180

Query: 181 FGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAERRGLVNQVVEGDELLKKAREVA 240
           FGIFPSWGLSQKL RIIGVNKAREVSLTAMPLTAEEAER+GLVN VV+G ELLKK RE+A
Sbjct: 181 FGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLVNHVVDGGELLKKTREIA 240

Query: 241 EAILKNNQDLVLRYKSVINDGLKLDLGQALALEKERAHVYYNGMTKEQFQKMQEFIAGRS 300
           EAILKNNQDLVLRYKSVINDGLKLDLG ALALEKERAH YY GMTKEQF+KMQEFIAGRS
Sbjct: 241 EAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYTGMTKEQFKKMQEFIAGRS 264

Query: 301 SKKPSKL 308
           SKKPSKL
Sbjct: 301 SKKPSKL 264

[Close]

BLAST of HG10010415 vs. ExPASy Swiss-Prot
Match: Q6NL24 (Probable enoyl-CoA hydratase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECHIA PE=1 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 2.2e-104
Identity = 202/305 (66.23%), Postives = 230/305 (75.41%), Query Frame = 0


Query: 1   MDRSSPENLILVNRESNGIAFVTINRPKSLNSLTKPMMTDLAQAFKSLDRDDSVRVIVLS 60
           MD++  ENLI V +ES GIA +TINRPKSLNSLT+ MM DLA+AFK +D D+SV+V++ +
Sbjct: 1   MDQTVSENLIQVKKESGGIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFT 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMERCRKPIIGAISGFAITAGFEMALAC 120
           GSGR+FCSGVDLTAAE VFKGDVKD E+DPV QMER RKPIIGAI+GFAITAGFE+ALAC
Sbjct: 61  GSGRSFCSGVDLTAAESVFKGDVKDPETDPVVQMERLRKPIIGAINGFAITAGFELALAC 120

Query: 121 DILIAAKGAKFIDTHARFLFFLILISEMKIVEITKIYWRCDSVLISDIEISGIKKNCCGR 180
           DIL+A++GAKF+DTHA                                           R
Sbjct: 121 DILVASRGAKFMDTHA-------------------------------------------R 180

Query: 181 FGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAERRGLVNQVVEGDELLKKAREVA 240
           FGIFPSWGLSQKL RIIG NKAREVSLT+MPLTA+ A + G VN VVE  E LKKARE+A
Sbjct: 181 FGIFPSWGLSQKLSRIIGANKAREVSLTSMPLTADVAGKLGFVNHVVEEGEALKKAREIA 240

Query: 241 EAILKNNQDLVLRYKSVINDGLKLDLGQALALEKERAHVYYNGMTKEQFQKMQEFIAGRS 300
           EAI+KN Q +VLR KSVINDGLKLDLG AL LEKERAH YY+GMTKEQF+KMQEFIAGR 
Sbjct: 241 EAIIKNEQGMVLRIKSVINDGLKLDLGHALTLEKERAHAYYSGMTKEQFRKMQEFIAGRG 262

Query: 301 SKKPS 306
           SKKPS
Sbjct: 301 SKKPS 262

[Close]

BLAST of HG10010415 vs. ExPASy Swiss-Prot
Match: P52046 (Short-chain-enoyl-CoA hydratase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=crt PE=1 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.7e-27
Identity = 88/296 (29.73%), Postives = 150/296 (50.68%), Query Frame = 0


Query: 8   NLILVNRESNGIAFVTINRPKSLNSLTKPMMTDLAQAFKSLDRDDSVRVIVLSGSG-RAF 67
           N +++ +E   +A VTINRPK+LN+L    + ++      ++ D  V  ++L+G+G ++F
Sbjct: 4   NNVILEKEGK-VAVVTINRPKALNALNSDTLKEMDYVIGEIENDSEVLAVILTGAGEKSF 63

Query: 68  CSGVDLTAAEDV--FKGDVKDVESDPV-AQMERCRKPIIGAISGFAITAGFEMALACDIL 127
            +G D++  +++   +G    +  + V  ++E   KP+I A++GFA+  G E+A++CDI 
Sbjct: 64  VAGADISEMKEMNTIEGRKFGILGNKVFRRLELLEKPVIAAVNGFALGGGCEIAMSCDIR 123

Query: 128 IAAKGAKFIDTHARFLFFLILISEMKIVEITKIYWRCDSVLISDIEISGIKKNCCGRFGI 187
           IA+  A+F                                     E+           GI
Sbjct: 124 IASSNARF----------------------------------GQPEVG---------LGI 183

Query: 188 FPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAERRGLVNQVVEGDELLKKAREVAEAI 247
            P +G +Q+L R++G+  A+++  TA  + A+EA R GLVN+VVE  EL+  A+E+A  I
Sbjct: 184 TPGFGGTQRLSRLVGMGMAKQLIFTAQNIKADEALRIGLVNKVVEPSELMNTAKEIANKI 243

Query: 248 LKNNQDLVLRYKSVINDGLKLDLGQALALEKERAHVYYNGMTKEQFQKMQEFIAGR 300
           + N    V   K  IN G++ D+  ALA E E     ++  T++Q   M  FI  R
Sbjct: 244 VSNAPVAVKLSKQAINRGMQCDIDTALAFESEAFGECFS--TEDQKDAMTAFIEKR 253

[Close]

BLAST of HG10010415 vs. ExPASy Swiss-Prot
Match: Q1ZXF1 (Probable enoyl-CoA hydratase, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=echs1 PE=3 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 2.2e-27
Identity = 91/285 (31.93%), Postives = 141/285 (49.47%), Query Frame = 0


Query: 19  IAFVTINRPKSLNSLTKPMMTDLAQAFKSLDRDDSVRVIVLSGSGRAFCSGVDLTAAEDV 78
           +  VT+NRPKSLN+L+  +++++  A K    D  V  I+++GS +AF +G D+   E V
Sbjct: 34  VGLVTLNRPKSLNALSDGLISEINSAVKLFQEDKDVGSIIITGSEKAFAAGADIKEMEKV 93

Query: 79  FKGDVKDVESDPVAQ---MERCRKPIIGAISGFAITAGFEMALACDILIAAKGAKFIDTH 138
              D     +D +AQ   + + RKPII A++G+A+  G E+A+ CDI+IA + A F    
Sbjct: 94  TLPDA--YNNDLLAQWHDLTKIRKPIIAAVNGYALGGGCELAMMCDIIIAGEKAVF---- 153

Query: 139 ARFLFFLILISEMKIVEITKIYWRCDSVLISDIEISGIKKNCCGRFGIFPSWGLSQKLGR 198
                                            EI         + G  P  G +Q+L R
Sbjct: 154 ------------------------------GQPEI---------KLGTIPGCGGTQRLIR 213

Query: 199 IIGVNKAREVSLTAMPLTAEEAERRGLVNQVVEGDELLKKAREVAEAILKNNQDLVLRYK 258
            IG +KA E+ LT   LTA EAE+ GLV++VV  +ELL +A ++AE I   +Q  V   K
Sbjct: 214 AIGKSKAMELVLTGNNLTAVEAEKAGLVSKVVPVEELLTEATKMAEKIASYSQLTVAMAK 271

Query: 259 SVINDGLKLDLGQALALEKERAHVYYNGMTKEQFQKMQEFIAGRS 301
             +N   +L L + +  E+   H  +   T +Q + M  F+  R+
Sbjct: 274 EAVNASYELTLQEGIRFERRMFHSTFG--THDQKEGMNAFVEKRT 271

[Close]

BLAST of HG10010415 vs. ExPASy Swiss-Prot
Match: P64017 (Probable enoyl-CoA hydratase echA8 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=echA8 PE=3 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 8.4e-27
Identity = 87/292 (29.79%), Postives = 136/292 (46.58%), Query Frame = 0


Query: 10  ILVNRESNGIAFVTINRPKSLNSLTKPMMTDLAQAFKSLDRDDSVRVIVLSGSGRAFCSG 69
           ILV R+   +  +T+NRP++LN+L   +M ++  A   LD D  +  I+++GS +AF +G
Sbjct: 6   ILVERDQR-VGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSAKAFAAG 65

Query: 70  VDLTAAEDVFKGDVKDVESDPV-AQMERCRKPIIGAISGFAITAGFEMALACDILIAAKG 129
            D+    D+   D    +      ++   R P I A++G+A+  G E+A+ CD+LIAA  
Sbjct: 66  ADIKEMADLTFADAFTADFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADT 125

Query: 130 AKFIDTHARFLFFLILISEMKIVEITKIYWRCDSVLISDIEISGIKKNCCGRFGIFPSWG 189
           AKF                                     EI         + G+ P  G
Sbjct: 126 AKF----------------------------------GQPEI---------KLGVLPGMG 185

Query: 190 LSQKLGRIIGVNKAREVSLTAMPLTAEEAERRGLVNQVVEGDELLKKAREVAEAILKNNQ 249
            SQ+L R IG  KA ++ LT   + A EAER GLV++VV  D+LL +AR  A  I + + 
Sbjct: 186 GSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRVVPADDLLTEARATATTISQMSA 245

Query: 250 DLVLRYKSVINDGLKLDLGQALALEKERAHVYYNGMTKEQFQKMQEFIAGRS 301
                 K  +N   +  L + L  E+   H  +   T++Q + M  FI  R+
Sbjct: 246 SAARMAKEAVNRAFESSLSEGLLYERRLFHSAF--ATEDQSEGMAAFIEKRA 251

[Close]

BLAST of HG10010415 vs. ExPASy Swiss-Prot
Match: P9WNN8 (Probable enoyl-CoA hydratase echA8 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=echA8 PE=3 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 8.4e-27
Identity = 87/292 (29.79%), Postives = 136/292 (46.58%), Query Frame = 0


Query: 10  ILVNRESNGIAFVTINRPKSLNSLTKPMMTDLAQAFKSLDRDDSVRVIVLSGSGRAFCSG 69
           ILV R+   +  +T+NRP++LN+L   +M ++  A   LD D  +  I+++GS +AF +G
Sbjct: 6   ILVERDQR-VGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSAKAFAAG 65

Query: 70  VDLTAAEDVFKGDVKDVESDPV-AQMERCRKPIIGAISGFAITAGFEMALACDILIAAKG 129
            D+    D+   D    +      ++   R P I A++G+A+  G E+A+ CD+LIAA  
Sbjct: 66  ADIKEMADLTFADAFTADFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADT 125

Query: 130 AKFIDTHARFLFFLILISEMKIVEITKIYWRCDSVLISDIEISGIKKNCCGRFGIFPSWG 189
           AKF                                     EI         + G+ P  G
Sbjct: 126 AKF----------------------------------GQPEI---------KLGVLPGMG 185

Query: 190 LSQKLGRIIGVNKAREVSLTAMPLTAEEAERRGLVNQVVEGDELLKKAREVAEAILKNNQ 249
            SQ+L R IG  KA ++ LT   + A EAER GLV++VV  D+LL +AR  A  I + + 
Sbjct: 186 GSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRVVPADDLLTEARATATTISQMSA 245

Query: 250 DLVLRYKSVINDGLKLDLGQALALEKERAHVYYNGMTKEQFQKMQEFIAGRS 301
                 K  +N   +  L + L  E+   H  +   T++Q + M  FI  R+
Sbjct: 246 SAARMAKEAVNRAFESSLSEGLLYERRLFHSAF--ATEDQSEGMAAFIEKRA 251

[Close]

BLAST of HG10010415 vs. ExPASy TrEMBL
Match: A0A1S3C8X4 (probable enoyl-CoA hydratase 1, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103497788 PE=4 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 1.0e-128
Identity = 252/307 (82.08%), Postives = 260/307 (84.69%), Query Frame = 0


Query: 1   MDRSSPENLILVNRESNGIAFVTINRPKSLNSLTKPMMTDLAQAFKSLDRDDSVRVIVLS 60
           MDRSSPENLILVNRESNGIAF+TINRPKSLNSLT+PMM DLA+AFKSLDRDDSVRVI+LS
Sbjct: 1   MDRSSPENLILVNRESNGIAFITINRPKSLNSLTRPMMIDLARAFKSLDRDDSVRVIILS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMERCRKPIIGAISGFAITAGFEMALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQME CRKPIIGAI+GFAITAGFE+ALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFLFFLILISEMKIVEITKIYWRCDSVLISDIEISGIKKNCCGR 180
           DILIAAKGAKFIDTHA                                           R
Sbjct: 121 DILIAAKGAKFIDTHA-------------------------------------------R 180

Query: 181 FGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAERRGLVNQVVEGDELLKKAREVA 240
           FGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAE+RGLVNQVVEG ELLKKAREVA
Sbjct: 181 FGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLVNQVVEGSELLKKAREVA 240

Query: 241 EAILKNNQDLVLRYKSVINDGLKLDLGQALALEKERAHVYYNGMTKEQFQKMQEFIAGRS 300
           EAILKNNQDLVLRYKSVINDGLKLDLGQALALEKERAH YYNGMTKEQFQKMQEFIAGRS
Sbjct: 241 EAILKNNQDLVLRYKSVINDGLKLDLGQALALEKERAHAYYNGMTKEQFQKMQEFIAGRS 264

Query: 301 SKKPSKL 308
           SK+PSKL
Sbjct: 301 SKRPSKL 264

[Close]

BLAST of HG10010415 vs. ExPASy TrEMBL
Match: A0A0A0KCM0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G330970 PE=4 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 2.8e-126
Identity = 249/307 (81.11%), Postives = 256/307 (83.39%), Query Frame = 0


Query: 1   MDRSSPENLILVNRESNGIAFVTINRPKSLNSLTKPMMTDLAQAFKSLDRDDSVRVIVLS 60
           MDRSS ENLILVNRESNGIAF+TINRPKSLNSLTK MM DLA AFKSLDRDDSVRVI+LS
Sbjct: 1   MDRSSSENLILVNRESNGIAFITINRPKSLNSLTKHMMADLAHAFKSLDRDDSVRVIILS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMERCRKPIIGAISGFAITAGFEMALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQME CRKPIIGAI+GFAITAGFE+ALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFLFFLILISEMKIVEITKIYWRCDSVLISDIEISGIKKNCCGR 180
           DILIAAKGAKFIDTHA                                           R
Sbjct: 121 DILIAAKGAKFIDTHA-------------------------------------------R 180

Query: 181 FGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAERRGLVNQVVEGDELLKKAREVA 240
           FGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAE+RGLVNQVVEG ELLKKAREVA
Sbjct: 181 FGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLVNQVVEGSELLKKAREVA 240

Query: 241 EAILKNNQDLVLRYKSVINDGLKLDLGQALALEKERAHVYYNGMTKEQFQKMQEFIAGRS 300
           EAILKNNQDLV+RYKSVINDGLKLDLGQAL LEKERAH YYNGMTKEQFQKMQEFIAGRS
Sbjct: 241 EAILKNNQDLVVRYKSVINDGLKLDLGQALTLEKERAHAYYNGMTKEQFQKMQEFIAGRS 264

Query: 301 SKKPSKL 308
           SK+PSKL
Sbjct: 301 SKRPSKL 264

[Close]

BLAST of HG10010415 vs. ExPASy TrEMBL
Match: A0A6J1H7Z0 (probable enoyl-CoA hydratase 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111460389 PE=4 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 5.0e-123
Identity = 241/307 (78.50%), Postives = 253/307 (82.41%), Query Frame = 0


Query: 1   MDRSSPENLILVNRESNGIAFVTINRPKSLNSLTKPMMTDLAQAFKSLDRDDSVRVIVLS 60
           MDRSSPE LILVNRE NGIAFVTINRPKSLNSLT+ MM DLAQAFK+LDRD+SVRVIVLS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKTLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMERCRKPIIGAISGFAITAGFEMALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVE+DPV QMERCRKPIIGA++GFA+TAGFE+ALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAVTAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFLFFLILISEMKIVEITKIYWRCDSVLISDIEISGIKKNCCGR 180
           DILIAAKGAKFIDTHA                                           R
Sbjct: 121 DILIAAKGAKFIDTHA-------------------------------------------R 180

Query: 181 FGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAERRGLVNQVVEGDELLKKAREVA 240
           FGIFPSWGLSQKL RIIGVNKAREVSLTAMPLTAEEAER+GLVN VV+G ELLKK RE+A
Sbjct: 181 FGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLVNHVVDGGELLKKTREIA 240

Query: 241 EAILKNNQDLVLRYKSVINDGLKLDLGQALALEKERAHVYYNGMTKEQFQKMQEFIAGRS 300
           EAILKNNQDLVLRYKSVINDGLKLDLG ALALEKERAH YYNGMTKEQF+KMQEFIAGRS
Sbjct: 241 EAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNGMTKEQFKKMQEFIAGRS 264

Query: 301 SKKPSKL 308
           SKKPSKL
Sbjct: 301 SKKPSKL 264

[Close]

BLAST of HG10010415 vs. ExPASy TrEMBL
Match: A0A6J1L4H0 (probable enoyl-CoA hydratase 1, peroxisomal isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499066 PE=4 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 3.2e-122
Identity = 241/307 (78.50%), Postives = 252/307 (82.08%), Query Frame = 0


Query: 1   MDRSSPENLILVNRESNGIAFVTINRPKSLNSLTKPMMTDLAQAFKSLDRDDSVRVIVLS 60
           MDRSS E LILVNRE NGIAFVTINRPKSLNSLT+ MM DLAQAFKSLDRD+SVRVIVLS
Sbjct: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMERCRKPIIGAISGFAITAGFEMALAC 120
           G+GRAFCSGVDLTAAEDVFKGDVKDVE+DPV QMERCRKPIIGA++GFAITAGFE+ALAC
Sbjct: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFLFFLILISEMKIVEITKIYWRCDSVLISDIEISGIKKNCCGR 180
           DILIAAKGAKFIDTHA                                           R
Sbjct: 121 DILIAAKGAKFIDTHA-------------------------------------------R 180

Query: 181 FGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAERRGLVNQVVEGDELLKKAREVA 240
           FGIFPSWGLSQKL RIIGVNKAREVSLTAMPLTAEEAER+GLVN VV+G ELLKK RE+A
Sbjct: 181 FGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLVNHVVDGGELLKKTREIA 240

Query: 241 EAILKNNQDLVLRYKSVINDGLKLDLGQALALEKERAHVYYNGMTKEQFQKMQEFIAGRS 300
           EAILKNNQDLVLRYKSVINDGLKLDLG ALALEKERAH YYNGMTKEQF+KMQEFIAGRS
Sbjct: 241 EAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNGMTKEQFKKMQEFIAGRS 264

Query: 301 SKKPSKL 308
           SKKPSKL
Sbjct: 301 SKKPSKL 264

[Close]

BLAST of HG10010415 vs. ExPASy TrEMBL
Match: A0A6J1L1R6 (probable enoyl-CoA hydratase 1, peroxisomal isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499066 PE=4 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 2.7e-121
Identity = 240/307 (78.18%), Postives = 251/307 (81.76%), Query Frame = 0


Query: 1   MDRSSPENLILVNRESNGIAFVTINRPKSLNSLTKPMMTDLAQAFKSLDRDDSVRVIVLS 60
           MDRSS E LILVNRE NGIAFVTINRPKSLNSLT+ MM DLAQAFKSLDRD+SVRVIVLS
Sbjct: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMERCRKPIIGAISGFAITAGFEMALAC 120
           G+GRAFCSGVDLTAAEDVFKGDVKDVE+DPV QMERCRKPIIGA++GFAITAGFE+ALAC
Sbjct: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFLFFLILISEMKIVEITKIYWRCDSVLISDIEISGIKKNCCGR 180
           DILIAAKGAKFIDTHA                                           R
Sbjct: 121 DILIAAKGAKFIDTHA-------------------------------------------R 180

Query: 181 FGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAERRGLVNQVVEGDELLKKAREVA 240
           F IFPSWGLSQKL RIIGVNKAREVSLTAMPLTAEEAER+GLVN VV+G ELLKK RE+A
Sbjct: 181 FRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLVNHVVDGGELLKKTREIA 240

Query: 241 EAILKNNQDLVLRYKSVINDGLKLDLGQALALEKERAHVYYNGMTKEQFQKMQEFIAGRS 300
           EAILKNNQDLVLRYKSVINDGLKLDLG ALALEKERAH YYNGMTKEQF+KMQEFIAGRS
Sbjct: 241 EAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNGMTKEQFKKMQEFIAGRS 264

Query: 301 SKKPSKL 308
           SKKPSKL
Sbjct: 301 SKKPSKL 264

[Close]

BLAST of HG10010415 vs. TAIR 10
Match: AT4G16210.1 (enoyl-CoA hydratase/isomerase A )

HSP 1 Score: 380.2 bits (975), Expect = 1.6e-105
Identity = 202/305 (66.23%), Postives = 230/305 (75.41%), Query Frame = 0


Query: 1   MDRSSPENLILVNRESNGIAFVTINRPKSLNSLTKPMMTDLAQAFKSLDRDDSVRVIVLS 60
           MD++  ENLI V +ES GIA +TINRPKSLNSLT+ MM DLA+AFK +D D+SV+V++ +
Sbjct: 1   MDQTVSENLIQVKKESGGIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFT 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMERCRKPIIGAISGFAITAGFEMALAC 120
           GSGR+FCSGVDLTAAE VFKGDVKD E+DPV QMER RKPIIGAI+GFAITAGFE+ALAC
Sbjct: 61  GSGRSFCSGVDLTAAESVFKGDVKDPETDPVVQMERLRKPIIGAINGFAITAGFELALAC 120

Query: 121 DILIAAKGAKFIDTHARFLFFLILISEMKIVEITKIYWRCDSVLISDIEISGIKKNCCGR 180
           DIL+A++GAKF+DTHA                                           R
Sbjct: 121 DILVASRGAKFMDTHA-------------------------------------------R 180

Query: 181 FGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAERRGLVNQVVEGDELLKKAREVA 240
           FGIFPSWGLSQKL RIIG NKAREVSLT+MPLTA+ A + G VN VVE  E LKKARE+A
Sbjct: 181 FGIFPSWGLSQKLSRIIGANKAREVSLTSMPLTADVAGKLGFVNHVVEEGEALKKAREIA 240

Query: 241 EAILKNNQDLVLRYKSVINDGLKLDLGQALALEKERAHVYYNGMTKEQFQKMQEFIAGRS 300
           EAI+KN Q +VLR KSVINDGLKLDLG AL LEKERAH YY+GMTKEQF+KMQEFIAGR 
Sbjct: 241 EAIIKNEQGMVLRIKSVINDGLKLDLGHALTLEKERAHAYYSGMTKEQFRKMQEFIAGRG 262

Query: 301 SKKPS 306
           SKKPS
Sbjct: 301 SKKPS 262

[Close]

BLAST of HG10010415 vs. TAIR 10
Match: AT4G16800.1 (ATP-dependent caseinolytic (Clp) protease/crotonase family protein )

HSP 1 Score: 92.4 bits (228), Expect = 6.6e-19
Identity = 75/278 (26.98%), Postives = 126/278 (45.32%), Query Frame = 0


Query: 4   SSPENLILVNRES---NGIAFVTINRPKSLNSLTKPMMTDLAQAFKSLDRDDSVRVIVL- 63
           +SP   + +NR S   +GI  V ++RP + N++ K M+  L  AF+S+ +D+S RV+++ 
Sbjct: 37  TSPPEFVKLNRLSGSDSGIIEVNLDRPVTKNAINKEMLKSLQNAFESIHQDNSARVVMIR 96

Query: 64  SGSGRAFCSGVDLTAAEDVFKGDVKDVESD---PVAQMERCRKPIIGAISGFAITAGFEM 123
           S     FC+G DL     +   +V    +      + +E    P I AI G A+  G EM
Sbjct: 97  SLVPGVFCAGADLKERRTMSPSEVHTYVNSLRYMFSFIEALSIPTIAAIEGAALGGGLEM 156

Query: 124 ALACDILIAAKGAKFIDTHARFLFFLILISEMKIVEITKIYWRCDSVLISDIEISGIKKN 183
           ALACD+ I  + A F                                        G+ + 
Sbjct: 157 ALACDLRICGENAVF----------------------------------------GLPET 216

Query: 184 CCGRFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAERRGLVNQVVEGDELLKKA 243
                 I P  G +Q+L R++G + ++E+  T   + A EA  +GLVN  V   E  +KA
Sbjct: 217 ---GLAIIPGAGGTQRLSRLVGRSVSKELIFTGRKIDAIEAANKGLVNICVTAGEAHEKA 271

Query: 244 REVAEAILKNNQDLVLRYKSVINDGLKLDLGQALALEK 275
            E+A+ I +     +   K  I++G++ ++   L +E+
Sbjct: 277 IEMAQQINEKGPLAIKMAKKAIDEGIETNMASGLEVEE 271

[Close]

BLAST of HG10010415 vs. TAIR 10
Match: AT5G43280.1 (delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 )

HSP 1 Score: 87.4 bits (215), Expect = 2.1e-17
Identity = 76/288 (26.39%), Postives = 128/288 (44.44%), Query Frame = 0


Query: 1   MDRSSPENLILVNRESNGIAF-VTINRPKSLNSLTKPMMTDLAQAFKSLDRDDSVRVIVL 60
           M   S + L ++ + ++   F + INRP  LN+L+     +  +A  SLD++  V VI+L
Sbjct: 1   MTMESYKTLEIIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIIL 60

Query: 61  SGSGRAFCSGVDLTAAEDV----------------FKGDVKDVESDPVAQMERCRKPIIG 120
           SG+G+ FCSG+DL +   +                 +  +K +++  +  +E+CRKP+I 
Sbjct: 61  SGAGKHFCSGIDLNSLSSISTQSSSGNDRGRSSEQLRRKIKSMQA-AITAIEQCRKPVIA 120

Query: 121 AISGFAITAGFEMALACDILIAAKGAKFIDTHARFLFFLILISEMKIVEITKIYWRCDSV 180
           AI G  I  G ++  ACDI   ++ A F                  I E+       D  
Sbjct: 121 AIHGACIGGGVDLITACDIRYCSEDAFF-----------------SIKEV-------DLA 180

Query: 181 LISDIEISGIKKNCCGRFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAERRGLV 240
           +++D+                   G  Q+L  I+G   A E++LTA   +  EA+  GLV
Sbjct: 181 IVADL-------------------GTLQRLPSIVGYANAMELALTARRFSGSEAKDLGLV 240

Query: 241 NQVV-EGDELLKKAREVAEAILKNNQDLVLRYKSVINDGLKLDLGQAL 271
           ++V     EL      +AE I   +   V   K+V+    ++ + Q L
Sbjct: 241 SKVFGSKSELDNGVTTIAEGIGGKSPLAVTGTKAVLLRSREVSVEQGL 244

[Close]

BLAST of HG10010415 vs. TAIR 10
Match: AT5G43280.2 (delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 )

HSP 1 Score: 87.4 bits (215), Expect = 2.1e-17
Identity = 66/243 (27.16%), Postives = 111/243 (45.68%), Query Frame = 0


Query: 1   MDRSSPENLILVNRESNGIAF-VTINRPKSLNSLTKPMMTDLAQAFKSLDRDDSVRVIVL 60
           M   S + L ++ + ++   F + INRP  LN+L+     +  +A  SLD++  V VI+L
Sbjct: 1   MTMESYKTLEIIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIIL 60

Query: 61  SGSGRAFCSGVDLTAAEDV----------------FKGDVKDVESDPVAQMERCRKPIIG 120
           SG+G+ FCSG+DL +   +                 +  +K +++  +  +E+CRKP+I 
Sbjct: 61  SGAGKHFCSGIDLNSLSSISTQSSSGNDRGRSSEQLRRKIKSMQA-AITAIEQCRKPVIA 120

Query: 121 AISGFAITAGFEMALACDILIAAKGAKFIDTHARFLFFLILISEMKIVEITKIYWRCDSV 180
           AI G  I  G ++  ACDI   ++ A F                  I E+       D  
Sbjct: 121 AIHGACIGGGVDLITACDIRYCSEDAFF-----------------SIKEV-------DLA 180

Query: 181 LISDIEISGIKKNCCGRFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAERRGLV 227
           +++D+                   G  Q+L  I+G   A E++LTA   +  EA+  GLV
Sbjct: 181 IVADL-------------------GTLQRLPSIVGYANAMELALTARRFSGSEAKDLGLV 199

[Close]

BLAST of HG10010415 vs. TAIR 10
Match: AT4G29010.1 (Enoyl-CoA hydratase/isomerase family )

HSP 1 Score: 86.3 bits (212), Expect = 4.8e-17
Identity = 61/230 (26.52%), Postives = 114/230 (49.57%), Query Frame = 0


Query: 16  SNGIAFVTINRPKSLNSLTKPMMTDLAQAFKSLDRDDSVRVIVLSGSGRAFCSGVDLTAA 75
           ++G+A +TI+ P  +NSL  P+++ L + F+  ++ + V+ IVL G+   F  G D+   
Sbjct: 13  NDGVAVITISNP-PVNSLASPIISGLKEKFRDANQRNDVKAIVLIGNNGRFSGGFDINVF 72

Query: 76  EDVFK-GDVK---DVESDPVAQ-MERCRKPIIGAISGFAITAGFEMALACDILIAAKGAK 135
           + V K GD+    +V  + V   ME  RKP++ A+ G A+  G E+A+AC   +AA  A 
Sbjct: 73  QQVHKTGDLSLMPEVSVELVCNLMEDSRKPVVAAVEGLALGGGLELAMACHARVAAPKA- 132

Query: 136 FIDTHARFLFFLILISEMKIVEITKIYWRCDSVLISDIEISGIKKNCCGRFGIFPSWGLS 195
                           ++ + E+T                           G+ P +G +
Sbjct: 133 ----------------QLGLPELT--------------------------LGVIPGFGGT 192

Query: 196 QKLGRIIGVNKAREVSLTAMPLTAEEAERRGLVNQVVEGDELLKKAREVA 241
           Q+L R++G+ KA ++ L +  +++EE  + GL++ +V   ++L  +R+ A
Sbjct: 193 QRLPRLVGLAKATDMILLSKSISSEEGHKLGLIDALVPPGDVLSTSRKWA 198

The following BLAST results are available for this feature:

BLAST of HG10010415 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008458352.1	2.1e-128	82.08	PREDICTED: probable enoyl-CoA hydratase 1, peroxisomal [Cucumis melo]	[more]
XP_038876166.1	5.8e-126	81.11	probable enoyl-CoA hydratase 1, peroxisomal [Benincasa hispida]	[more]
XP_004148801.1	5.8e-126	81.11	probable enoyl-CoA hydratase 1, peroxisomal [Cucumis sativus] >KGN47460.1 hypoth...	[more]
XP_022959404.1	1.0e-122	78.50	probable enoyl-CoA hydratase 1, peroxisomal [Cucurbita moschata]	[more]
KAG6575117.1	3.9e-122	78.50	putative enoyl-CoA hydratase 1, peroxisomal, partial [Cucurbita argyrosperma sub...	[more]

BLAST of HG10010415 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NL24	2.2e-104	66.23	Probable enoyl-CoA hydratase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=E...	[more]
P52046	1.7e-27	29.73	Short-chain-enoyl-CoA hydratase OS=Clostridium acetobutylicum (strain ATCC 824 /...	[more]
Q1ZXF1	2.2e-27	31.93	Probable enoyl-CoA hydratase, mitochondrial OS=Dictyostelium discoideum OX=44689...	[more]
P64017	8.4e-27	29.79	Probable enoyl-CoA hydratase echA8 OS=Mycobacterium bovis (strain ATCC BAA-935 /...	[more]
P9WNN8	8.4e-27	29.79	Probable enoyl-CoA hydratase echA8 OS=Mycobacterium tuberculosis (strain CDC 155...	[more]

BLAST of HG10010415 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C8X4	1.0e-128	82.08	probable enoyl-CoA hydratase 1, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103497...	[more]
A0A0A0KCM0	2.8e-126	81.11	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G330970 PE=4 SV=1	[more]
A0A6J1H7Z0	5.0e-123	78.50	probable enoyl-CoA hydratase 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1L4H0	3.2e-122	78.50	probable enoyl-CoA hydratase 1, peroxisomal isoform X1 OS=Cucurbita maxima OX=36...	[more]
A0A6J1L1R6	2.7e-121	78.18	probable enoyl-CoA hydratase 1, peroxisomal isoform X2 OS=Cucurbita maxima OX=36...	[more]

BLAST of HG10010415 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16210.1	1.6e-105	66.23	enoyl-CoA hydratase/isomerase A	[more]
AT4G16800.1	6.6e-19	26.98	ATP-dependent caseinolytic (Clp) protease/crotonase family protein	[more]
AT5G43280.1	2.1e-17	26.39	delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1	[more]
AT5G43280.2	2.1e-17	27.16	delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1	[more]
AT4G29010.1	4.8e-17	26.52	Enoyl-CoA hydratase/isomerase family	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.90.226.10		coord: 159..303 e-value: 1.1E-22 score: 82.6
None	No IPR available	GENE3D	3.90.226.10		coord: 9..146 e-value: 6.2E-40 score: 139.1
None	No IPR available	PANTHER	PTHR43802	ENOYL-COA HYDRATASE	coord: 177..303 coord: 5..145
None	No IPR available	CDD	cd06558	crotonase-like	coord: 10..243 e-value: 1.00242E-62 score: 195.087
IPR001753	Enoyl-CoA hydratase/isomerase	PFAM	PF00378	ECH_1	coord: 15..137 e-value: 6.6E-32 score: 110.8 coord: 180..281 e-value: 1.2E-16 score: 60.8
IPR029045	ClpP/crotonase-like domain superfamily	SUPERFAMILY	52096	ClpP/crotonase	coord: 180..300
IPR029045	ClpP/crotonase-like domain superfamily	SUPERFAMILY	52096	ClpP/crotonase	coord: 8..138

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10010415.1	HG10010415.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003824	catalytic activity