HG10010367 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10010367 vs. NCBI nr
Match: XP_038875843.1 (probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida])

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 534/575 (92.87%), Postives = 558/575 (97.04%), Query Frame = 0

Query: 1   MSNGFANAEGDKQKKKIAIISVSSLILVAMVVAVTVGVNNAPHEEHDDGHGGETHEISST 60
           MSNGFANAEGDK+KKK+AIISVSSLILVAMVVAVTVGVNNAP EEH DGHGGETHEISST
Sbjct: 1   MSNGFANAEGDKKKKKMAIISVSSLILVAMVVAVTVGVNNAPKEEHGDGHGGETHEISST 60

Query: 61  TKAIKAICQPTDYKESCEKSLLAEAGNTTDPKELVKVGFKIATRNLNEAIRNSTTLKELA 120
           TKAI+AICQPTDYK+ CEK+L+AEAGNTTDPKEL KVG +IATRNLNEAIRNSTTL+ELA
Sbjct: 61  TKAIQAICQPTDYKDVCEKNLMAEAGNTTDPKELAKVGLRIATRNLNEAIRNSTTLQELA 120

Query: 121 KDPRTNQALQNCHELLEYAIDDLNESFERIGTFQMSKLDEFVGDLKIWLSGALTYEQTCL 180
           KDPRTNQALQNC ELLEYAIDDLNESFERIGTFQMSKLDEFV DLKIWLSGALTYEQTCL
Sbjct: 121 KDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQTCL 180

Query: 181 DGFENTTGDAGVRMQEFLKSAQEMTSNGLAIVNELSSILGSLQIPGMTGRRLLEEDDEEM 240
           DGFENTTGDAGVRMQEFLKS+Q+MT+NGLAIVNELS +LGSLQIPGMTGRRLLE+D +EM
Sbjct: 181 DGFENTTGDAGVRMQEFLKSSQQMTTNGLAIVNELSGMLGSLQIPGMTGRRLLEDDADEM 240

Query: 241 PSWVSDGKRRLMQMQAAGSSVKPDLVVAQDGSGKYNTINAALADVPLKSNKTFVIYVKAG 300
           PSWVS+GKRRL  MQAA +S+KPDLVVAQDGSGKY TINAALADVPLKSNKTFVIYVKAG
Sbjct: 241 PSWVSEGKRRL--MQAAAASMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIYVKAG 300

Query: 301 IYKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNFIDGVQTFKTATFSAIGANFYARDMGFE 360
           IYKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNFIDG+QTFKTATF+AIGANFYARDMGFE
Sbjct: 301 IYKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNFIDGIQTFKTATFAAIGANFYARDMGFE 360

Query: 361 NTAGAAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAA 420
           NTAGAAKHQAVALRVQSDRSIFYNC+IDGYQDTLYAHAHRQFYRDC ISGTIDFVFGNAA
Sbjct: 361 NTAGAAKHQAVALRVQSDRSIFYNCKIDGYQDTLYAHAHRQFYRDCIISGTIDFVFGNAA 420

Query: 421 TIFQNCKLVVRKPMDNQQCIVTAHGRLERREPTALIFQSCHFMGDPAYLPFKNVNKAYLG 480
           TIFQNCKLVVRKPMDNQQCIVTAHGRL R+EPTALIFQSCHFMGDPAYLPFKN+NKAYLG
Sbjct: 421 TIFQNCKLVVRKPMDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKNINKAYLG 480

Query: 481 RPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNTGPGADESKRVKWRGI 540
           RPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQN GPGADESKRVKWRGI
Sbjct: 481 RPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGPGADESKRVKWRGI 540

Query: 541 KHITPQHAADFTPRRFIHGDAWIPAKGIPYSSGMM 576
           +HITPQHAAD+TPRRFI GDAWIPAKGIPYSSGMM
Sbjct: 541 RHITPQHAADYTPRRFIDGDAWIPAKGIPYSSGMM 573

BLAST of HG10010367 vs. NCBI nr
Match: XP_008458409.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo])

HSP 1 Score: 1074.7 bits (2778), Expect = 4.4e-310
Identity = 528/577 (91.51%), Postives = 553/577 (95.84%), Query Frame = 0

Query: 1   MSNGFANAEGDKQKKKIAIISVSSLILVAMVVAVTVGVNNAPHEEHDDGHGGE--THEIS 60
           MSNGFANAEGDK+KKKIAIISVSSLILVAMVVAVTVG+NNAP+EEH +G  G   T EIS
Sbjct: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGINNAPYEEHGEGDQGSKTTPEIS 60

Query: 61  STTKAIKAICQPTDYKESCEKSLLAEAGNTTDPKELVKVGFKIATRNLNEAIRNSTTLKE 120
           S+TKAI+AICQPTDYKE+CEKSL+AEAGNTTDPKELVKVGFKIATR+LNEAIRNSTTL+E
Sbjct: 61  SSTKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQE 120

Query: 121 LAKDPRTNQALQNCHELLEYAIDDLNESFERIGTFQMSKLDEFVGDLKIWLSGALTYEQT 180
           L+KDPRTNQALQNC ELLEYAIDDLNESFERIGTFQMSKLDEFV DLKIWLSGALTYEQT
Sbjct: 121 LSKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQT 180

Query: 181 CLDGFENTTGDAGVRMQEFLKSAQEMTSNGLAIVNELSSILGSLQIPGMTGRRLLEEDDE 240
           CLDGFENTTGDAG RMQEFLKSAQEMT+NGL IVNELS+ILGSLQIPGMTGRRLLE+DD 
Sbjct: 181 CLDGFENTTGDAGTRMQEFLKSAQEMTTNGLGIVNELSTILGSLQIPGMTGRRLLEDDDH 240

Query: 241 EMPSWVSDGKRRLMQMQAAGSSVKPDLVVAQDGSGKYNTINAALADVPLKSNKTFVIYVK 300
           EMPSW+SDGKRRLMQ  AAG  +KPDL+VAQDGSGKY TINAALADVPLKSNKTFVIYVK
Sbjct: 241 EMPSWISDGKRRLMQAGAAG--MKPDLIVAQDGSGKYKTINAALADVPLKSNKTFVIYVK 300

Query: 301 AGIYKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNFIDGVQTFKTATFSAIGANFYARDMG 360
           AGIYKEIVVIPK MTHLTMYGDGPTKT+VTGSLNFIDG+QTFKTATFSAIGANFYARDMG
Sbjct: 301 AGIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMG 360

Query: 361 FENTAGAAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGN 420
           FENTAG+AKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGN
Sbjct: 361 FENTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGN 420

Query: 421 AATIFQNCKLVVRKPMDNQQCIVTAHGRLERREPTALIFQSCHFMGDPAYLPFKNVNKAY 480
           AAT FQNCKLVVRKPMDNQQCIVTAHGR+ R+EPTALIFQ CHFMGDPAYLPFK++NKAY
Sbjct: 421 AATNFQNCKLVVRKPMDNQQCIVTAHGRMNRKEPTALIFQGCHFMGDPAYLPFKSINKAY 480

Query: 481 LGRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNTGPGADESKRVKWR 540
           LGRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQN GPGA E+KRVKWR
Sbjct: 481 LGRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGPGAVETKRVKWR 540

Query: 541 GIKHITPQHAADFTPRRFIHGDAWIPAKGIPYSSGMM 576
           GIKHITPQHAADFTPRRFI GDAWIPAKGIPYSSGMM
Sbjct: 541 GIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 575

BLAST of HG10010367 vs. NCBI nr
Match: XP_004150503.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucumis sativus] >KGN47423.1 hypothetical protein Csa_021732 [Cucumis sativus])

HSP 1 Score: 1065.4 bits (2754), Expect = 1.7e-307
Identity = 528/578 (91.35%), Postives = 554/578 (95.85%), Query Frame = 0

Query: 1   MSNGFANAEGDKQKKKIAIISVSSLILVAMVVAVTVGVNNAPH-EEH-DDGHGGE-THEI 60
           MSNGFANAEGDK+KKKIAIISVSSLILVAMVVAVTVGVNNAPH EEH DD HG E T EI
Sbjct: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEI 60

Query: 61  SSTTKAIKAICQPTDYKESCEKSLLAEAGNTTDPKELVKVGFKIATRNLNEAIRNSTTLK 120
           +S+TKAI+AICQPTDYKE+CEKSL AEAGNTTDPKELVKVGFKIATR+LNEAI+NSTTLK
Sbjct: 61  TSSTKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLK 120

Query: 121 ELAKDPRTNQALQNCHELLEYAIDDLNESFERIGTFQMSKLDEFVGDLKIWLSGALTYEQ 180
           ELAKDPRTNQALQNC ELLEYAIDDLN+SFERIGTFQMSKLD+FV DLKIWLSGALTYEQ
Sbjct: 121 ELAKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQ 180

Query: 181 TCLDGFENTTGDAGVRMQEFLKSAQEMTSNGLAIVNELSSILGSLQIPGMTGRRLLEEDD 240
           TCLDGFENTTGDAGVRMQEFLKSAQ+MT+NGL IVNELS+ILGSLQ+PGM+GRRLLE+DD
Sbjct: 181 TCLDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSGRRLLEDDD 240

Query: 241 EEMPSWVSDGKRRLMQMQAAGSSVKPDLVVAQDGSGKYNTINAALADVPLKSNKTFVIYV 300
            EMPSWVSDGKRRL  MQA  +++KPDLVVAQDGSGKY TINAALADVPLKSNKTFVI+V
Sbjct: 241 REMPSWVSDGKRRL--MQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHV 300

Query: 301 KAGIYKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNFIDGVQTFKTATFSAIGANFYARDM 360
           KAGIYKEIVVIPK MTHLTMYGDGPTKT+VTGSLNFIDG+QTFKTATFSAIGANFYARDM
Sbjct: 301 KAGIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDM 360

Query: 361 GFENTAGAAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFG 420
           GFENTAGAAKHQAVALRVQSDRSIF+NCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFG
Sbjct: 361 GFENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFG 420

Query: 421 NAATIFQNCKLVVRKPMDNQQCIVTAHGRLERREPTALIFQSCHFMGDPAYLPFKNVNKA 480
           NAAT FQNCKLVVRKP+DNQQCIVTAHGRL R+EPTALIFQSCHFMGDPAYLPFK +NKA
Sbjct: 421 NAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKA 480

Query: 481 YLGRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNTGPGADESKRVKW 540
           YLGRPWK+YSRTIIIGS IDDIIQPEGWLPWMGDFGLNTLFYAEVQN G GADESKRVKW
Sbjct: 481 YLGRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKW 540

Query: 541 RGIKHITPQHAADFTPRRFIHGDAWIPAKGIPYSSGMM 576
           RGIKHITPQHAADFTPRRFI GDAWIPAKGIPYSSGMM
Sbjct: 541 RGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576

BLAST of HG10010367 vs. NCBI nr
Match: XP_023549205.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 964.5 bits (2492), Expect = 4.0e-277
Identity = 472/585 (80.68%), Postives = 518/585 (88.55%), Query Frame = 0

Query: 1   MSNGFANAEGDKQKKKIAIISVSSLILVAMVVAVTVGVNNAPHEEHDDG----------H 60
           MSNGF +AEGDK+KKKIA+ISVSSLILVAMVVAVTVGVNNAP E+ DDG          H
Sbjct: 1   MSNGF-DAEGDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDH 60

Query: 61  GGETHEISSTTKAIKAICQPTDYKESCEKSLLAEAGNTTDPKELVKVGFKIATRNLNEAI 120
           G +  ++SS+TKAIK ICQPTDYKE+CE+SL+ EAGNTTDPKELVK GFKIA   LN AI
Sbjct: 61  GDDGEKVSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAI 120

Query: 121 RNSTTLKELAKDPRTNQALQNCHELLEYAIDDLNESFERIGTFQMSKLDEFVGDLKIWLS 180
           RNSTTL+ELA+DPR NQALQNC ELLEYAIDDL++S+ RIGTF +SK +EFV DLKIWLS
Sbjct: 121 RNSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLS 180

Query: 181 GALTYEQTCLDGFENTTGDAGVRMQEFLKSAQEMTSNGLAIVNELSSILGSLQIPGMTGR 240
            ALTYEQTCLDGFENTTGD+G RM+EFLKSAQEMT+NGL IV+ELS+ILG+L  P  TGR
Sbjct: 181 AALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSNILGTLHAPAATGR 240

Query: 241 RLLEEDDEEMPSWVSDGKRRLMQMQAAGSSVKPDLVVAQDGSGKYNTINAALADVPLKSN 300
           RLL   D +MP WVSDGKRRLMQM     ++KPDLVVAQDGSGKY TIN AL DVP+KSN
Sbjct: 241 RLL---DTDMPLWVSDGKRRLMQM--GTGAIKPDLVVAQDGSGKYKTINEALVDVPMKSN 300

Query: 301 KTFVIYVKAGIYKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNFIDGVQTFKTATFSAIGA 360
           KTFVIYVKAGIYKEIVVIPK MTHLTMYGDGPTK++VTG+LNF+DG+QTFKTATF+AIGA
Sbjct: 301 KTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGA 360

Query: 361 NFYARDMGFENTAGAAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISG 420
           NF+ RDMGF NTAG  KHQAVALRVQSDR+I YNCQIDGYQDTLYAHA+RQFYRDC ISG
Sbjct: 361 NFFVRDMGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISG 420

Query: 421 TIDFVFGNAATIFQNCKLVVRKPMDNQQCIVTAHGRLERREPTALIFQSCHFMGDPAYLP 480
           TIDFVFGN+A IFQNCKLVVRKP+DNQQCIVTAHGRL RREPTALIFQ CHFMGDPAYLP
Sbjct: 421 TIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPAYLP 480

Query: 481 FKNVNKAYLGRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNTGPGAD 540
           FK VNKAYLGRPWKQYSRTII+GSQIDD+IQPEGWLPWMGDF LNTLFYAEVQN GPGAD
Sbjct: 481 FKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGAD 540

Query: 541 ESKRVKWRGIKHITPQHAADFTPRRFIHGDAWIPAKGIPYSSGMM 576
           +S RVKWRGI+HITPQHAA +T RRFI GDAWIP +G+PYSSGMM
Sbjct: 541 QSHRVKWRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579

BLAST of HG10010367 vs. NCBI nr
Match: XP_023006255.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita maxima])

HSP 1 Score: 959.1 bits (2478), Expect = 1.7e-275
Identity = 472/606 (77.89%), Postives = 521/606 (85.97%), Query Frame = 0

Query: 1   MSNGFANAEGDKQKKKIAIISVSSLILVAMVVAVTVGVNNAPHEEHDDG----------- 60
           MSNGF +AEGDK+KKKIA+ISVSSLILVAMVVAVTVGVNNAPHE+ DDG           
Sbjct: 1   MSNGF-DAEGDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPHEDLDDGGGGGDLHDHGD 60

Query: 61  --------------------HGGETHEISSTTKAIKAICQPTDYKESCEKSLLAEAGNTT 120
                               HG +  +ISS+TKAIK ICQPTDYKE+CE+SL+ EAGNTT
Sbjct: 61  DGGDVHDHGDYGGGGGDAHDHGDDGEKISSSTKAIKTICQPTDYKETCEESLMVEAGNTT 120

Query: 121 DPKELVKVGFKIATRNLNEAIRNSTTLKELAKDPRTNQALQNCHELLEYAIDDLNESFER 180
           D KELVK GFKIA   LN AIRNSTTL+ELAKDPR NQALQNC ELLEYAIDDL++S+ R
Sbjct: 121 DLKELVKAGFKIAVEKLNAAIRNSTTLQELAKDPRANQALQNCRELLEYAIDDLHDSYHR 180

Query: 181 IGTFQMSKLDEFVGDLKIWLSGALTYEQTCLDGFENTTGDAGVRMQEFLKSAQEMTSNGL 240
           IGTF +SK +EFV DLKIWLS ALTYEQTCLDGFENTTGD+G RM+EFLKSAQEMT+NGL
Sbjct: 181 IGTFHVSKFEEFVNDLKIWLSAALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGL 240

Query: 241 AIVNELSSILGSLQIPGMTGRRLLEEDDEEMPSWVSDGKRRLMQMQAAGSSVKPDLVVAQ 300
            IV+ELS+ILG+L  P  TGRRLL   D +MP WVSDGKRRLMQ++    ++KPDLVVAQ
Sbjct: 241 GIVSELSNILGTLHEPAATGRRLL---DTDMPLWVSDGKRRLMQLET--GAIKPDLVVAQ 300

Query: 301 DGSGKYNTINAALADVPLKSNKTFVIYVKAGIYKEIVVIPKAMTHLTMYGDGPTKTIVTG 360
           DGSGKY TIN AL DVP+KSNKTFVIYVKAGIYKEIVVIPK MTHLTMYGDGPTK++VTG
Sbjct: 301 DGSGKYKTINEALVDVPMKSNKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTG 360

Query: 361 SLNFIDGVQTFKTATFSAIGANFYARDMGFENTAGAAKHQAVALRVQSDRSIFYNCQIDG 420
           +LNF+DG+QTFKTATF+AIGANF+ RDMGF N+AG  KHQAVALR+QSDR+I YNCQIDG
Sbjct: 361 NLNFVDGIQTFKTATFAAIGANFFVRDMGFVNSAGPTKHQAVALRIQSDRAIIYNCQIDG 420

Query: 421 YQDTLYAHAHRQFYRDCTISGTIDFVFGNAATIFQNCKLVVRKPMDNQQCIVTAHGRLER 480
           +QDTLYAHA+RQFYRDCTISGTIDFVFGN+A IFQNCKLVVRKP+DNQQCIVTAHGRL R
Sbjct: 421 FQDTLYAHANRQFYRDCTISGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNR 480

Query: 481 REPTALIFQSCHFMGDPAYLPFKNVNKAYLGRPWKQYSRTIIIGSQIDDIIQPEGWLPWM 540
           REPTALIFQ CHFMGDPAYLPFK VNKAYLGRPWKQYSRTII+GSQIDD+IQPEGWLPWM
Sbjct: 481 REPTALIFQGCHFMGDPAYLPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWM 540

Query: 541 GDFGLNTLFYAEVQNTGPGADESKRVKWRGIKHITPQHAADFTPRRFIHGDAWIPAKGIP 576
           GDFGLNTLFYAEVQN GPGAD+S RVKWRGI+HITPQHAA +T RRFI GDAWIP +G+P
Sbjct: 541 GDFGLNTLFYAEVQNRGPGADQSHRVKWRGIRHITPQHAASYTARRFIDGDAWIPVRGVP 600

BLAST of HG10010367 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 670.6 bits (1729), Expect = 1.6e-191
Identity = 312/575 (54.26%), Postives = 426/575 (74.09%), Query Frame = 0

Query: 1   MSNGFANAEGDKQKKKIAIISVSSLILVAMVVAVTVGVNNAPHEEHDDGHGGETHEISST 60
           MS G+   +  K+K++  +I++SS++L++MVVAVTVGV+   H    DG      E++++
Sbjct: 1   MSYGY--DDESKRKRRYIVITISSVLLISMVVAVTVGVSLNKH----DGDSKGKAEVNAS 60

Query: 61  TKAIKAICQPTDYKESCEKSLLAEAGNTTDPKELVKVGFKIATRNLNEAIRNSTTLKELA 120
            KA+K +C PTDY+++CE +L+    NTTDP ELVK  F +  + + +A + S T+ EL 
Sbjct: 61  VKAVKDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQ 120

Query: 121 KDPRTNQALQNCHELLEYAIDDLNESFERIGTFQMSKLDEFVGDLKIWLSGALTYEQTCL 180
           KD RT  AL  C EL++YA+D+L+ SFE +G F+   LDE + +L+IWLS A+++E+TCL
Sbjct: 121 KDSRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCL 180

Query: 181 DGFENTTGDAGVRMQEFLKSAQEMTSNGLAIVNELSSILGSLQIPGMTGRRLLEEDDEEM 240
           +GF+ T G+AG  M++ LK+A E+T NGLAI++E+S+ +G +QIPG+  RRLL E     
Sbjct: 181 EGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLAEG---F 240

Query: 241 PSWVSDGKRRLMQMQAAGSSVKPDLVVAQDGSGKYNTINAALADVPLKSNKTFVIYVKAG 300
           PSWV    R+L+Q  AA S VKPD+VVAQDGSG+Y TIN AL  VP K N TFV+++KAG
Sbjct: 241 PSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAG 300

Query: 301 IYKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNFIDGVQTFKTATFSAIGANFYARDMGFE 360
           +YKE V + K M+HL   GDGP KTI++G+ N+ DG+ T++TAT + +G  F A+++GFE
Sbjct: 301 LYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFE 360

Query: 361 NTAGAAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAA 420
           NTAGA KHQAVA+RVQSD SIF+NC+ DGYQDTLY H+HRQF+RDCTISGTIDF+FG+AA
Sbjct: 361 NTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAA 420

Query: 421 TIFQNCKLVVRKPMDNQQCIVTAHGRLERREPTALIFQSCHFMGDPAYLPFKNVNKAYLG 480
            +FQNC L+VRKP+ NQ C +TAHGR + RE T  +FQ C   G+P YL  K  +KAYLG
Sbjct: 421 AVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLG 480

Query: 481 RPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNTGPGADESKRVKWRGI 540
           RPWK+YSRTII+ + I D +QP+GW PW+GDFGL TLFY+EVQNTGPG+  + RV W GI
Sbjct: 481 RPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGI 540

Query: 541 KHITPQHAADFTPRRFIHGDAWIPAKGIPYSSGMM 576
           K ++ +    FTP ++I GD WIP KG+PY++G++
Sbjct: 541 KTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

BLAST of HG10010367 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 4.0e-187
Identity = 316/574 (55.05%), Postives = 421/574 (73.34%), Query Frame = 0

Query: 1   MSNGFANAEGDKQKKKIAIISVSSLILVAMVVAVTVGVNNAPHEEHDDGHGGETHEISST 60
           MS G+ + E  K+KK+  IIS+SS++L++MVVAVT+GV+        D  G E  EI+++
Sbjct: 1   MSYGY-DDEDAKRKKRYVIISISSVLLISMVVAVTIGVS----VNKSDNAGDE--EITTS 60

Query: 61  TKAIKAICQPTDYKESCEKSLLAEAGNTTDPKELVKVGFKIATRNLNEAIRNSTTLKELA 120
            KAIK +C PTDYKE+CE +L  +A +T+DP ELVK  F    + +++  + S T+ EL 
Sbjct: 61  VKAIKDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQ 120

Query: 121 KDPRTNQALQNCHELLEYAIDDLNESFERIGTFQMSKLDEFVGDLKIWLSGALTYEQTCL 180
           KDPR   AL  C EL++YAI +L++SFE +G F+  K+DE +  L+IWLS  +++EQTCL
Sbjct: 121 KDPRAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCL 180

Query: 181 DGFENTTGDAGVRMQEFLKSAQEMTSNGLAIVNELSSILGSLQIPGMTGRRLLEEDDEEM 240
           DGF+ T G+AG  +++ LK+A ++T NGLA+V E+S+ LG +QIP M  RRLL    +E 
Sbjct: 181 DGFQGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLL---SQEF 240

Query: 241 PSWVSDGKRRLMQMQAAGSSVKPDLVVAQDGSGKYNTINAALADVPLKSNKTFVIYVKAG 300
           PSW+    RRL  + A  S VKPD+VVAQDGSG+Y TIN AL  VP K N TFV+++K G
Sbjct: 241 PSWMDARARRL--LNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEG 300

Query: 301 IYKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNFIDGVQTFKTATFSAIGANFYARDMGFE 360
           IYKE V + ++MTHL   GDGP KT+++GS ++ DG+ T+KTAT + +G +F A+++ FE
Sbjct: 301 IYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFE 360

Query: 361 NTAGAAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAA 420
           NTAGA KHQAVA+RV +D SIFYNC+ DGYQDTLYAH+HRQFYRDCTISGTIDF+FG+AA
Sbjct: 361 NTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAA 420

Query: 421 TIFQNCKLVVRKPMDNQQCIVTAHGRLERREPTALIFQSCHFMGDPAYLPFKNVNKAYLG 480
            +FQNC L+VRKP+ NQ C +TAHGR + RE T  + Q C  +G+P YL  K  +K YLG
Sbjct: 421 AVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLG 480

Query: 481 RPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNTGPGADESKRVKWRGI 540
           RPWK+YSRTII+ + I D + PEGW PW+G+FGLNTLFY+EVQNTGPGA  +KRV W GI
Sbjct: 481 RPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGI 540

Query: 541 KHITPQHAADFTPRRFIHGDAWIPAKGIPYSSGM 575
           K ++ +    FTP ++I GDAWIP KG+PY  G+
Sbjct: 541 KKLSDEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of HG10010367 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 4.9e-169
Identity = 282/571 (49.39%), Postives = 400/571 (70.05%), Query Frame = 0

Query: 9   EGDKQKKKIAIISVSSLILVAMVVAVTVGVNNAPHEEHDDGHGGETHEISSTTKAIKAIC 68
           +G+ +KKK  I  V + +LV MVVA  VG+  + +  H +       +I + T A++A+C
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVA--VGITTSRNTSHSEKI--VPVQIKTATTAVEAVC 63

Query: 69  QPTDYKESCEKSLLAEAGNTTDPKELVKVGFKIATRNLNEAIRNSTT--LKELAKDPRTN 128
            PTDYKE+C  SL+  + ++T P +L+K+GF +  R++ ++I+ ++     + A D  T 
Sbjct: 64  APTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTK 123

Query: 129 QALQNCHELLEYAIDDLNESFERIGTFQMSKLDEFVGDLKIWLSGALTYEQTCLDGFENT 188
            AL+ C +L+  A DDL +  +    F + ++++FV DL++WLSG++ Y+QTC+D FE T
Sbjct: 124 GALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEET 183

Query: 189 TGDAGVRMQEFLKSAQEMTSNGLAIVNELSSILGSLQIPGMTG------RRLLEEDDEEM 248
                  MQ+  K+++E+TSNGLA++  +S++LG   + G+TG      R+LL  +D  +
Sbjct: 184 NSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAED-GI 243

Query: 249 PSWVSDGKRRLMQMQAAGSSVKPDLVVAQDGSGKYNTINAALADVPLKSNKTFVIYVKAG 308
           PSWV    RRLM   A    VK ++VVA DGSG+Y TIN AL  VP  + K FVIY+K G
Sbjct: 244 PSWVGPNTRRLM---ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQG 303

Query: 309 IYKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNFIDG-VQTFKTATFSAIGANFYARDMGF 368
           +Y E V + K MTH+T  GDGPTKT +TGSLN+  G V+T+ TAT +  G NF A+++GF
Sbjct: 304 VYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGF 363

Query: 369 ENTAGAAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA 428
           ENTAG   HQAVALRV +D ++FYNCQIDGYQDTLY H+HRQF+RDCT+SGT+DF+FG+ 
Sbjct: 364 ENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDG 423

Query: 429 ATIFQNCKLVVRKPMDNQQCIVTAHGRLERREPTALIFQSCHFMGDPAYLPFKNVNKAYL 488
             + QNC +VVRKPM +Q C++TA GR ++RE T L+ Q+CH  G+PAY+P K++NKAYL
Sbjct: 424 IVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYL 483

Query: 489 GRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNTGPGADESKRVKWRG 548
           GRPWK++SRTII+G+ IDD+I P GWLPW GDF LNTL+YAE +N GPG+++++RVKW G
Sbjct: 484 GRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPG 543

Query: 549 IKHITPQHAADFTPRRFIHGDAWIPAKGIPY 571
           IK ++P+ A  FTP RF+ G+ WIP   +PY
Sbjct: 544 IKKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

BLAST of HG10010367 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 554.3 bits (1427), Expect = 1.6e-156
Identity = 276/575 (48.00%), Postives = 393/575 (68.35%), Query Frame = 0

Query: 8   AEGDKQKKKIAIISVSSLILVAMVVAVTVGVNNAPHEEHDDGHGGETHEISSTTKAIKAI 67
           ++GDK+KK I   SVS  +   +++ V+V V  + H   DD    E H I  TTKA++A+
Sbjct: 3   SDGDKKKKFIVAGSVSGFL---VIMVVSVAVVTSKHSPKDD----ENH-IRKTTKAVQAV 62

Query: 68  CQPTDYKESCEKSLLAEAGNTTDPKELVKVGFKIATRNLNEAI-RNSTTLKELA-KDPRT 127
           C PTD+K++C  SL+  + ++ DP +L+K+GFK+  +++NE++ + S  +K  A K+P  
Sbjct: 63  CAPTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEA 122

Query: 128 NQALQNCHELLEYAIDDLNESFERIGTFQMSKLDEFVGDLKIWLSGALTYEQTCLDGFEN 187
             A + C +L+  AIDDL +  +    F + +++ FV DL++WLSG++ ++QTC+D F  
Sbjct: 123 KGAFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGE 182

Query: 188 TTGDAGVRMQEFLKSAQEMTSNGLAIVNELSSILGSLQIPGMTG------RRLLEEDDEE 247
              +    M +  K+++E++SN LA+V  +S+++ +  + G+TG      R+LL  +D  
Sbjct: 183 IKSNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTED-S 242

Query: 248 MPSWVSDGKRRLMQMQAAG-SSVKPDLVVAQDGSGKYNTINAALADVPLKSNKTFVIYVK 307
           +P+WV    RRLM  Q  G   VK + VVAQDG+G++ TI  AL  VP  +   F+I++K
Sbjct: 243 IPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFIIHIK 302

Query: 308 AGIYKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNF-IDGVQTFKTATFSAIGANFYARDM 367
            GIYKE V + K M H+T  GDGP KT++TGSLNF I  V+TF TAT +  G +F A+++
Sbjct: 303 EGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNI 362

Query: 368 GFENTAGAAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFG 427
           G ENTAG    QAVALRV +D ++F++CQIDG+QDTLY H+HRQFYRDCT+SGT+DF+FG
Sbjct: 363 GIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFG 422

Query: 428 NAATIFQNCKLVVRKPMDNQQCIVTAHGRLERREPTALIFQSCHFMGDPAYLPFKNVNKA 487
           +A  I QNCK+VVRKP   Q C+VTA GR   RE T L+   CH  GDPAY+P K+VNKA
Sbjct: 423 DAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKA 482

Query: 488 YLGRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNTGPGADESKRVKW 547
           YLGRPWK++SRTII+ + IDD+I P GWLPW GDF L TL+YAE  NTGPG+++++RVKW
Sbjct: 483 YLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKW 542

Query: 548 RGIKHITPQHAADFTPRRFIHGDAWIPAKGIPYSS 573
            GIK +TPQ A  +T  RF+ GD WIP   +PY++
Sbjct: 543 PGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

BLAST of HG10010367 vs. ExPASy Swiss-Prot
Match: O23447 (Putative pectinesterase/pectinesterase inhibitor 43 OS=Arabidopsis thaliana OX=3702 GN=PME43 PE=2 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 2.7e-135
Identity = 264/703 (37.55%), Postives = 379/703 (53.91%), Query Frame = 0

Query: 16  KIAIISVSSLILVAMVVAVTVGVNNAPHEEHDDGHGGETHEISSTTKAIKAICQPTDYKE 75
           K  ++ V++LI+ AMV+ V     N+P  + ++ H      I+ TT +I  IC  TDYK+
Sbjct: 3   KYVLLGVTALIM-AMVICVEANDGNSPSRKMEEVHRESKLMITKTTVSI--ICASTDYKQ 62

Query: 76  SCEKSLLAEAGNTTDPKELVKVGFKIATRNLNEAI-RNSTTLKELA-KDPRTNQALQNCH 135
            C  SL      + DP+ L++  F +A  ++   I R    LK  A  D  T +AL  C 
Sbjct: 63  DCTTSLATV--RSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTCR 122

Query: 136 ELLEYAIDDLNESFERIGTFQMSKLDEFVGDLKIWLSGALTYEQTCLDGFENTTGDAGVR 195
           EL++ AIDDL ++ ++   F  ++L +FV DL +WLSG++TY+QTC+DGFE    +A V 
Sbjct: 123 ELMDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAVM 182

Query: 196 MQEFLKSAQEMTSNGLAIVNELSSILGSLQIP---------------------------- 255
           M+  ++  Q +TSNGLAI   L  +L + +IP                            
Sbjct: 183 MERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGSSQ 242

Query: 256 -----------------------------------------------------------G 315
                                                                       
Sbjct: 243 DSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKKSA 302

Query: 316 MTGRRLLEE--------------------------------------------------- 375
            +G + L++                                                   
Sbjct: 303 FSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDSLK 362

Query: 376 -----DDEEMPSWVSDGKRRLMQMQAAGSSVKPDLVVAQDGSGKYNTINAALADVPLKSN 435
                ++ E P WV+   RRL+  +   + +K ++VVA+DGSGK  TI  ALA VP+K+ 
Sbjct: 363 DRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVPMKNT 422

Query: 436 KTFVIYVKAGIYKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNFI-DGVQTFKTATFSAIG 495
           K FVI++K G+YKE V + K M H+   GDGPTKT++TG + F+ D V T++TA+ +  G
Sbjct: 423 KKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAVNG 482

Query: 496 ANFYARDMGFENTAGAAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTIS 555
             F A+D+GFENTAGAA+HQAVALRV +D ++F+NC ++GYQDTLY H HRQFYR+C +S
Sbjct: 483 DYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCRVS 542

Query: 556 GTIDFVFGNAATIFQNCKLVVRKPMDNQQCIVTAHGRLERREPTALIFQSCHFMGDPAYL 573
           GTIDFVFG+A  +FQNC+ V+R+PM++QQCIVTA GR +RRE T ++  +    GD +YL
Sbjct: 543 GTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDASYL 602

BLAST of HG10010367 vs. ExPASy TrEMBL
Match: A0A1S3C7B6 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497833 PE=3 SV=1)

HSP 1 Score: 1074.7 bits (2778), Expect = 2.1e-310
Identity = 528/577 (91.51%), Postives = 553/577 (95.84%), Query Frame = 0

Query: 1   MSNGFANAEGDKQKKKIAIISVSSLILVAMVVAVTVGVNNAPHEEHDDGHGGE--THEIS 60
           MSNGFANAEGDK+KKKIAIISVSSLILVAMVVAVTVG+NNAP+EEH +G  G   T EIS
Sbjct: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGINNAPYEEHGEGDQGSKTTPEIS 60

Query: 61  STTKAIKAICQPTDYKESCEKSLLAEAGNTTDPKELVKVGFKIATRNLNEAIRNSTTLKE 120
           S+TKAI+AICQPTDYKE+CEKSL+AEAGNTTDPKELVKVGFKIATR+LNEAIRNSTTL+E
Sbjct: 61  SSTKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQE 120

Query: 121 LAKDPRTNQALQNCHELLEYAIDDLNESFERIGTFQMSKLDEFVGDLKIWLSGALTYEQT 180
           L+KDPRTNQALQNC ELLEYAIDDLNESFERIGTFQMSKLDEFV DLKIWLSGALTYEQT
Sbjct: 121 LSKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQT 180

Query: 181 CLDGFENTTGDAGVRMQEFLKSAQEMTSNGLAIVNELSSILGSLQIPGMTGRRLLEEDDE 240
           CLDGFENTTGDAG RMQEFLKSAQEMT+NGL IVNELS+ILGSLQIPGMTGRRLLE+DD 
Sbjct: 181 CLDGFENTTGDAGTRMQEFLKSAQEMTTNGLGIVNELSTILGSLQIPGMTGRRLLEDDDH 240

Query: 241 EMPSWVSDGKRRLMQMQAAGSSVKPDLVVAQDGSGKYNTINAALADVPLKSNKTFVIYVK 300
           EMPSW+SDGKRRLMQ  AAG  +KPDL+VAQDGSGKY TINAALADVPLKSNKTFVIYVK
Sbjct: 241 EMPSWISDGKRRLMQAGAAG--MKPDLIVAQDGSGKYKTINAALADVPLKSNKTFVIYVK 300

Query: 301 AGIYKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNFIDGVQTFKTATFSAIGANFYARDMG 360
           AGIYKEIVVIPK MTHLTMYGDGPTKT+VTGSLNFIDG+QTFKTATFSAIGANFYARDMG
Sbjct: 301 AGIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMG 360

Query: 361 FENTAGAAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGN 420
           FENTAG+AKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGN
Sbjct: 361 FENTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGN 420

Query: 421 AATIFQNCKLVVRKPMDNQQCIVTAHGRLERREPTALIFQSCHFMGDPAYLPFKNVNKAY 480
           AAT FQNCKLVVRKPMDNQQCIVTAHGR+ R+EPTALIFQ CHFMGDPAYLPFK++NKAY
Sbjct: 421 AATNFQNCKLVVRKPMDNQQCIVTAHGRMNRKEPTALIFQGCHFMGDPAYLPFKSINKAY 480

Query: 481 LGRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNTGPGADESKRVKWR 540
           LGRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQN GPGA E+KRVKWR
Sbjct: 481 LGRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGPGAVETKRVKWR 540

Query: 541 GIKHITPQHAADFTPRRFIHGDAWIPAKGIPYSSGMM 576
           GIKHITPQHAADFTPRRFI GDAWIPAKGIPYSSGMM
Sbjct: 541 GIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 575

BLAST of HG10010367 vs. ExPASy TrEMBL
Match: A0A0A0KD24 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G318700 PE=3 SV=1)

HSP 1 Score: 1065.4 bits (2754), Expect = 8.0e-308
Identity = 528/578 (91.35%), Postives = 554/578 (95.85%), Query Frame = 0

Query: 1   MSNGFANAEGDKQKKKIAIISVSSLILVAMVVAVTVGVNNAPH-EEH-DDGHGGE-THEI 60
           MSNGFANAEGDK+KKKIAIISVSSLILVAMVVAVTVGVNNAPH EEH DD HG E T EI
Sbjct: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEI 60

Query: 61  SSTTKAIKAICQPTDYKESCEKSLLAEAGNTTDPKELVKVGFKIATRNLNEAIRNSTTLK 120
           +S+TKAI+AICQPTDYKE+CEKSL AEAGNTTDPKELVKVGFKIATR+LNEAI+NSTTLK
Sbjct: 61  TSSTKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLK 120

Query: 121 ELAKDPRTNQALQNCHELLEYAIDDLNESFERIGTFQMSKLDEFVGDLKIWLSGALTYEQ 180
           ELAKDPRTNQALQNC ELLEYAIDDLN+SFERIGTFQMSKLD+FV DLKIWLSGALTYEQ
Sbjct: 121 ELAKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQ 180

Query: 181 TCLDGFENTTGDAGVRMQEFLKSAQEMTSNGLAIVNELSSILGSLQIPGMTGRRLLEEDD 240
           TCLDGFENTTGDAGVRMQEFLKSAQ+MT+NGL IVNELS+ILGSLQ+PGM+GRRLLE+DD
Sbjct: 181 TCLDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSGRRLLEDDD 240

Query: 241 EEMPSWVSDGKRRLMQMQAAGSSVKPDLVVAQDGSGKYNTINAALADVPLKSNKTFVIYV 300
            EMPSWVSDGKRRL  MQA  +++KPDLVVAQDGSGKY TINAALADVPLKSNKTFVI+V
Sbjct: 241 REMPSWVSDGKRRL--MQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHV 300

Query: 301 KAGIYKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNFIDGVQTFKTATFSAIGANFYARDM 360
           KAGIYKEIVVIPK MTHLTMYGDGPTKT+VTGSLNFIDG+QTFKTATFSAIGANFYARDM
Sbjct: 301 KAGIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDM 360

Query: 361 GFENTAGAAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFG 420
           GFENTAGAAKHQAVALRVQSDRSIF+NCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFG
Sbjct: 361 GFENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFG 420

Query: 421 NAATIFQNCKLVVRKPMDNQQCIVTAHGRLERREPTALIFQSCHFMGDPAYLPFKNVNKA 480
           NAAT FQNCKLVVRKP+DNQQCIVTAHGRL R+EPTALIFQSCHFMGDPAYLPFK +NKA
Sbjct: 421 NAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKA 480

Query: 481 YLGRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNTGPGADESKRVKW 540
           YLGRPWK+YSRTIIIGS IDDIIQPEGWLPWMGDFGLNTLFYAEVQN G GADESKRVKW
Sbjct: 481 YLGRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKW 540

Query: 541 RGIKHITPQHAADFTPRRFIHGDAWIPAKGIPYSSGMM 576
           RGIKHITPQHAADFTPRRFI GDAWIPAKGIPYSSGMM
Sbjct: 541 RGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576

BLAST of HG10010367 vs. ExPASy TrEMBL
Match: A0A6J1KZN0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499042 PE=3 SV=1)

HSP 1 Score: 959.1 bits (2478), Expect = 8.1e-276
Identity = 472/606 (77.89%), Postives = 521/606 (85.97%), Query Frame = 0

Query: 1   MSNGFANAEGDKQKKKIAIISVSSLILVAMVVAVTVGVNNAPHEEHDDG----------- 60
           MSNGF +AEGDK+KKKIA+ISVSSLILVAMVVAVTVGVNNAPHE+ DDG           
Sbjct: 1   MSNGF-DAEGDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPHEDLDDGGGGGDLHDHGD 60

Query: 61  --------------------HGGETHEISSTTKAIKAICQPTDYKESCEKSLLAEAGNTT 120
                               HG +  +ISS+TKAIK ICQPTDYKE+CE+SL+ EAGNTT
Sbjct: 61  DGGDVHDHGDYGGGGGDAHDHGDDGEKISSSTKAIKTICQPTDYKETCEESLMVEAGNTT 120

Query: 121 DPKELVKVGFKIATRNLNEAIRNSTTLKELAKDPRTNQALQNCHELLEYAIDDLNESFER 180
           D KELVK GFKIA   LN AIRNSTTL+ELAKDPR NQALQNC ELLEYAIDDL++S+ R
Sbjct: 121 DLKELVKAGFKIAVEKLNAAIRNSTTLQELAKDPRANQALQNCRELLEYAIDDLHDSYHR 180

Query: 181 IGTFQMSKLDEFVGDLKIWLSGALTYEQTCLDGFENTTGDAGVRMQEFLKSAQEMTSNGL 240
           IGTF +SK +EFV DLKIWLS ALTYEQTCLDGFENTTGD+G RM+EFLKSAQEMT+NGL
Sbjct: 181 IGTFHVSKFEEFVNDLKIWLSAALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGL 240

Query: 241 AIVNELSSILGSLQIPGMTGRRLLEEDDEEMPSWVSDGKRRLMQMQAAGSSVKPDLVVAQ 300
            IV+ELS+ILG+L  P  TGRRLL   D +MP WVSDGKRRLMQ++    ++KPDLVVAQ
Sbjct: 241 GIVSELSNILGTLHEPAATGRRLL---DTDMPLWVSDGKRRLMQLET--GAIKPDLVVAQ 300

Query: 301 DGSGKYNTINAALADVPLKSNKTFVIYVKAGIYKEIVVIPKAMTHLTMYGDGPTKTIVTG 360
           DGSGKY TIN AL DVP+KSNKTFVIYVKAGIYKEIVVIPK MTHLTMYGDGPTK++VTG
Sbjct: 301 DGSGKYKTINEALVDVPMKSNKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTG 360

Query: 361 SLNFIDGVQTFKTATFSAIGANFYARDMGFENTAGAAKHQAVALRVQSDRSIFYNCQIDG 420
           +LNF+DG+QTFKTATF+AIGANF+ RDMGF N+AG  KHQAVALR+QSDR+I YNCQIDG
Sbjct: 361 NLNFVDGIQTFKTATFAAIGANFFVRDMGFVNSAGPTKHQAVALRIQSDRAIIYNCQIDG 420

Query: 421 YQDTLYAHAHRQFYRDCTISGTIDFVFGNAATIFQNCKLVVRKPMDNQQCIVTAHGRLER 480
           +QDTLYAHA+RQFYRDCTISGTIDFVFGN+A IFQNCKLVVRKP+DNQQCIVTAHGRL R
Sbjct: 421 FQDTLYAHANRQFYRDCTISGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNR 480

Query: 481 REPTALIFQSCHFMGDPAYLPFKNVNKAYLGRPWKQYSRTIIIGSQIDDIIQPEGWLPWM 540
           REPTALIFQ CHFMGDPAYLPFK VNKAYLGRPWKQYSRTII+GSQIDD+IQPEGWLPWM
Sbjct: 481 REPTALIFQGCHFMGDPAYLPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWM 540

Query: 541 GDFGLNTLFYAEVQNTGPGADESKRVKWRGIKHITPQHAADFTPRRFIHGDAWIPAKGIP 576
           GDFGLNTLFYAEVQN GPGAD+S RVKWRGI+HITPQHAA +T RRFI GDAWIP +G+P
Sbjct: 541 GDFGLNTLFYAEVQNRGPGADQSHRVKWRGIRHITPQHAASYTARRFIDGDAWIPVRGVP 600

BLAST of HG10010367 vs. ExPASy TrEMBL
Match: A0A6J1H4H8 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460411 PE=3 SV=1)

HSP 1 Score: 956.1 bits (2470), Expect = 6.9e-275
Identity = 468/585 (80.00%), Postives = 515/585 (88.03%), Query Frame = 0

Query: 1   MSNGFANAEGDKQKKKIAIISVSSLILVAMVVAVTVGVNNAPHEEHDDG----------H 60
           MSNGF +AE DK+KKKIA+ISVSSLILVAMVVAVTVGVNNAP E+ DDG          H
Sbjct: 1   MSNGF-DAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDH 60

Query: 61  GGETHEISSTTKAIKAICQPTDYKESCEKSLLAEAGNTTDPKELVKVGFKIATRNLNEAI 120
           G +  +ISS+TKAIK ICQPTDYKE+CE+SL+ EAGNTTDPKELVK GFKIA   LN AI
Sbjct: 61  GDDAEKISSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAI 120

Query: 121 RNSTTLKELAKDPRTNQALQNCHELLEYAIDDLNESFERIGTFQMSKLDEFVGDLKIWLS 180
           RNSTTL+ELA+DPR NQALQNC ELLEYAIDDL++S+ RIGTF +SK +EFV DLKIWLS
Sbjct: 121 RNSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLS 180

Query: 181 GALTYEQTCLDGFENTTGDAGVRMQEFLKSAQEMTSNGLAIVNELSSILGSLQIPGMTGR 240
            ALTYEQTCLDGFENTTGD+G RM+EFLKSAQEMT+NGL IV+ELS ILG+L  P  TGR
Sbjct: 181 AALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLDAPAATGR 240

Query: 241 RLLEEDDEEMPSWVSDGKRRLMQMQAAGSSVKPDLVVAQDGSGKYNTINAALADVPLKSN 300
           RLL   D ++P WVSDGKRRLMQ+     ++KPDLVVAQDGSGKY TIN AL  VP+KSN
Sbjct: 241 RLL---DTDIPLWVSDGKRRLMQV--GTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSN 300

Query: 301 KTFVIYVKAGIYKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNFIDGVQTFKTATFSAIGA 360
           KTFVIYVKAGIYKEIVVIPK MTHLTMYGDGPTK++VTG+LNF+DG+QTFKTATF+AIGA
Sbjct: 301 KTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGA 360

Query: 361 NFYARDMGFENTAGAAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISG 420
           NF+ RDMGF NTAG  KHQAVALRVQSDR+I YNCQIDGYQDTLYAHA+RQFYRDC ISG
Sbjct: 361 NFFVRDMGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISG 420

Query: 421 TIDFVFGNAATIFQNCKLVVRKPMDNQQCIVTAHGRLERREPTALIFQSCHFMGDPAYLP 480
           TIDFVFGN+A IFQNCKLVVRKP+DNQQCIVTAHGRL RREPTALIFQ CHFMGDP+YLP
Sbjct: 421 TIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLP 480

Query: 481 FKNVNKAYLGRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNTGPGAD 540
           FK VNKAYLGRPWKQYSRTII+GSQIDD+IQPEGWLPWMGDF LNTLFYAEVQN GPGAD
Sbjct: 481 FKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGAD 540

Query: 541 ESKRVKWRGIKHITPQHAADFTPRRFIHGDAWIPAKGIPYSSGMM 576
           +S RVKWRGI+HITPQHAA +T RRFI GDAWIP +G+PYSSGMM
Sbjct: 541 QSHRVKWRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579

BLAST of HG10010367 vs. ExPASy TrEMBL
Match: A0A6J1KJ31 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111494433 PE=3 SV=1)

HSP 1 Score: 890.6 bits (2300), Expect = 3.5e-255
Identity = 438/577 (75.91%), Postives = 494/577 (85.62%), Query Frame = 0

Query: 1   MSNGFANAEGDKQKKKIAIISVSSLILVAMVVAVTVGVNNAPH--EEHDDGHGGETHEIS 60
           MSNGF +AEGD +KKK+A + VSSL LVAMVVAVTVGVNN+ H  E   DGHG    EIS
Sbjct: 1   MSNGF-DAEGDNKKKKMAFVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEIS 60

Query: 61  STTKAIKAICQPTDYKESCEKSLLAEAGNTTDPKELVKVGFKIATRNLNEAIRNSTTLKE 120
           S+TKAI+ IC+PTDYKE+CEKSL++E  NTTDP+ELVK+GFK+    LN AIRNSTTL+ 
Sbjct: 61  SSTKAIQTICRPTDYKETCEKSLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQA 120

Query: 121 LAKDPRTNQALQNCHELLEYAIDDLNESFERIGTFQMSKLDEFVGDLKIWLSGALTYEQT 180
           LAK+PRTNQALQNC ELLEYAIDD+ +SF RIGTFQM+KLD+FV DL IWLSGALTYEQT
Sbjct: 121 LAKEPRTNQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSIWLSGALTYEQT 180

Query: 181 CLDGFENTTGDAGVRMQEFLKSAQEMTSNGLAIVNELSSILGSLQIPGMTGRRLLEEDDE 240
           CLDGFENTTGDAG RM+ FLKSAQEMTSNGLAIV EL ++LGSLQ     GRRLL  D  
Sbjct: 181 CLDGFENTTGDAGERMKMFLKSAQEMTSNGLAIVTELKNMLGSLQ-GSKAGRRLLAGD-- 240

Query: 241 EMPSWVSDGKRRLMQMQAAGSSVKPDLVVAQDGSGKYNTINAALADVPLKSNKTFVIYVK 300
           E+PSWVS  KRRLMQ+    S++ P++VVAQDGSGKY TIN AL DVP KSN TFVIYVK
Sbjct: 241 EVPSWVSSEKRRLMQLPR--SAITPNVVVAQDGSGKYKTINEALVDVPKKSNITFVIYVK 300

Query: 301 AGIYKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNFIDGVQTFKTATFSAIGANFYARDMG 360
           AGIYKE V+IPKA+TH+TMYGDGPTKTIV+G+LNF+DG+QTFKTATF+ +GANF+ RDMG
Sbjct: 301 AGIYKETVLIPKALTHVTMYGDGPTKTIVSGALNFVDGIQTFKTATFAVVGANFFCRDMG 360

Query: 361 FENTAGAAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGN 420
           FENTAG+AKHQAVALRVQSDRSIF+NCQ+DGYQDTLYA  HRQFYRDC +SGTIDFVFGN
Sbjct: 361 FENTAGSAKHQAVALRVQSDRSIFFNCQMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGN 420

Query: 421 AATIFQNCKLVVRKPMDNQQCIVTAHGRLERREPTALIFQSCHFMGDPAYLPFKNVNKAY 480
           +A +FQNC LVVRKP+ NQQCIVTA GRLE REPT  +FQ CH MGDPAYLPFK VNKAY
Sbjct: 421 SAAVFQNCNLVVRKPLANQQCIVTAQGRLEAREPTGFVFQKCHLMGDPAYLPFKAVNKAY 480

Query: 481 LGRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNTGPGADESKRVKWR 540
           LGRPWKQYSRTII+GS+IDD+IQPEGWLPWMGDFGLN+LFYAE QN GPGAD + RVKW+
Sbjct: 481 LGRPWKQYSRTIIMGSEIDDLIQPEGWLPWMGDFGLNSLFYAEFQNKGPGADLTNRVKWQ 540

Query: 541 GIKHITPQHAADFTPRRFIHGDAWIPAKGIPYSSGMM 576
           GIK +T Q AADFTP+ FI GDAWI   G+PYS GMM
Sbjct: 541 GIKTLTAQQAADFTPKVFIDGDAWILNTGVPYSPGMM 571

BLAST of HG10010367 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 670.6 bits (1729), Expect = 1.1e-192
Identity = 312/575 (54.26%), Postives = 426/575 (74.09%), Query Frame = 0

Query: 1   MSNGFANAEGDKQKKKIAIISVSSLILVAMVVAVTVGVNNAPHEEHDDGHGGETHEISST 60
           MS G+   +  K+K++  +I++SS++L++MVVAVTVGV+   H    DG      E++++
Sbjct: 1   MSYGY--DDESKRKRRYIVITISSVLLISMVVAVTVGVSLNKH----DGDSKGKAEVNAS 60

Query: 61  TKAIKAICQPTDYKESCEKSLLAEAGNTTDPKELVKVGFKIATRNLNEAIRNSTTLKELA 120
            KA+K +C PTDY+++CE +L+    NTTDP ELVK  F +  + + +A + S T+ EL 
Sbjct: 61  VKAVKDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQ 120

Query: 121 KDPRTNQALQNCHELLEYAIDDLNESFERIGTFQMSKLDEFVGDLKIWLSGALTYEQTCL 180
           KD RT  AL  C EL++YA+D+L+ SFE +G F+   LDE + +L+IWLS A+++E+TCL
Sbjct: 121 KDSRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCL 180

Query: 181 DGFENTTGDAGVRMQEFLKSAQEMTSNGLAIVNELSSILGSLQIPGMTGRRLLEEDDEEM 240
           +GF+ T G+AG  M++ LK+A E+T NGLAI++E+S+ +G +QIPG+  RRLL E     
Sbjct: 181 EGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLAEG---F 240

Query: 241 PSWVSDGKRRLMQMQAAGSSVKPDLVVAQDGSGKYNTINAALADVPLKSNKTFVIYVKAG 300
           PSWV    R+L+Q  AA S VKPD+VVAQDGSG+Y TIN AL  VP K N TFV+++KAG
Sbjct: 241 PSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAG 300

Query: 301 IYKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNFIDGVQTFKTATFSAIGANFYARDMGFE 360
           +YKE V + K M+HL   GDGP KTI++G+ N+ DG+ T++TAT + +G  F A+++GFE
Sbjct: 301 LYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFE 360

Query: 361 NTAGAAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAA 420
           NTAGA KHQAVA+RVQSD SIF+NC+ DGYQDTLY H+HRQF+RDCTISGTIDF+FG+AA
Sbjct: 361 NTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAA 420

Query: 421 TIFQNCKLVVRKPMDNQQCIVTAHGRLERREPTALIFQSCHFMGDPAYLPFKNVNKAYLG 480
            +FQNC L+VRKP+ NQ C +TAHGR + RE T  +FQ C   G+P YL  K  +KAYLG
Sbjct: 421 AVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLG 480

Query: 481 RPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNTGPGADESKRVKWRGI 540
           RPWK+YSRTII+ + I D +QP+GW PW+GDFGL TLFY+EVQNTGPG+  + RV W GI
Sbjct: 481 RPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGI 540

Query: 541 KHITPQHAADFTPRRFIHGDAWIPAKGIPYSSGMM 576
           K ++ +    FTP ++I GD WIP KG+PY++G++
Sbjct: 541 KTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

BLAST of HG10010367 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 656.0 bits (1691), Expect = 2.8e-188
Identity = 316/574 (55.05%), Postives = 421/574 (73.34%), Query Frame = 0

Query: 1   MSNGFANAEGDKQKKKIAIISVSSLILVAMVVAVTVGVNNAPHEEHDDGHGGETHEISST 60
           MS G+ + E  K+KK+  IIS+SS++L++MVVAVT+GV+        D  G E  EI+++
Sbjct: 1   MSYGY-DDEDAKRKKRYVIISISSVLLISMVVAVTIGVS----VNKSDNAGDE--EITTS 60

Query: 61  TKAIKAICQPTDYKESCEKSLLAEAGNTTDPKELVKVGFKIATRNLNEAIRNSTTLKELA 120
            KAIK +C PTDYKE+CE +L  +A +T+DP ELVK  F    + +++  + S T+ EL 
Sbjct: 61  VKAIKDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQ 120

Query: 121 KDPRTNQALQNCHELLEYAIDDLNESFERIGTFQMSKLDEFVGDLKIWLSGALTYEQTCL 180
           KDPR   AL  C EL++YAI +L++SFE +G F+  K+DE +  L+IWLS  +++EQTCL
Sbjct: 121 KDPRAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCL 180

Query: 181 DGFENTTGDAGVRMQEFLKSAQEMTSNGLAIVNELSSILGSLQIPGMTGRRLLEEDDEEM 240
           DGF+ T G+AG  +++ LK+A ++T NGLA+V E+S+ LG +QIP M  RRLL    +E 
Sbjct: 181 DGFQGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLL---SQEF 240

Query: 241 PSWVSDGKRRLMQMQAAGSSVKPDLVVAQDGSGKYNTINAALADVPLKSNKTFVIYVKAG 300
           PSW+    RRL  + A  S VKPD+VVAQDGSG+Y TIN AL  VP K N TFV+++K G
Sbjct: 241 PSWMDARARRL--LNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEG 300

Query: 301 IYKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNFIDGVQTFKTATFSAIGANFYARDMGFE 360
           IYKE V + ++MTHL   GDGP KT+++GS ++ DG+ T+KTAT + +G +F A+++ FE
Sbjct: 301 IYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFE 360

Query: 361 NTAGAAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAA 420
           NTAGA KHQAVA+RV +D SIFYNC+ DGYQDTLYAH+HRQFYRDCTISGTIDF+FG+AA
Sbjct: 361 NTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAA 420

Query: 421 TIFQNCKLVVRKPMDNQQCIVTAHGRLERREPTALIFQSCHFMGDPAYLPFKNVNKAYLG 480
            +FQNC L+VRKP+ NQ C +TAHGR + RE T  + Q C  +G+P YL  K  +K YLG
Sbjct: 421 AVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLG 480

Query: 481 RPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNTGPGADESKRVKWRGI 540
           RPWK+YSRTII+ + I D + PEGW PW+G+FGLNTLFY+EVQNTGPGA  +KRV W GI
Sbjct: 481 RPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGI 540

Query: 541 KHITPQHAADFTPRRFIHGDAWIPAKGIPYSSGM 575
           K ++ +    FTP ++I GDAWIP KG+PY  G+
Sbjct: 541 KKLSDEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of HG10010367 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 595.9 bits (1535), Expect = 3.5e-170
Identity = 282/571 (49.39%), Postives = 400/571 (70.05%), Query Frame = 0

Query: 9   EGDKQKKKIAIISVSSLILVAMVVAVTVGVNNAPHEEHDDGHGGETHEISSTTKAIKAIC 68
           +G+ +KKK  I  V + +LV MVVA  VG+  + +  H +       +I + T A++A+C
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVA--VGITTSRNTSHSEKI--VPVQIKTATTAVEAVC 63

Query: 69  QPTDYKESCEKSLLAEAGNTTDPKELVKVGFKIATRNLNEAIRNSTT--LKELAKDPRTN 128
            PTDYKE+C  SL+  + ++T P +L+K+GF +  R++ ++I+ ++     + A D  T 
Sbjct: 64  APTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTK 123

Query: 129 QALQNCHELLEYAIDDLNESFERIGTFQMSKLDEFVGDLKIWLSGALTYEQTCLDGFENT 188
            AL+ C +L+  A DDL +  +    F + ++++FV DL++WLSG++ Y+QTC+D FE T
Sbjct: 124 GALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEET 183

Query: 189 TGDAGVRMQEFLKSAQEMTSNGLAIVNELSSILGSLQIPGMTG------RRLLEEDDEEM 248
                  MQ+  K+++E+TSNGLA++  +S++LG   + G+TG      R+LL  +D  +
Sbjct: 184 NSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAED-GI 243

Query: 249 PSWVSDGKRRLMQMQAAGSSVKPDLVVAQDGSGKYNTINAALADVPLKSNKTFVIYVKAG 308
           PSWV    RRLM   A    VK ++VVA DGSG+Y TIN AL  VP  + K FVIY+K G
Sbjct: 244 PSWVGPNTRRLM---ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQG 303

Query: 309 IYKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNFIDG-VQTFKTATFSAIGANFYARDMGF 368
           +Y E V + K MTH+T  GDGPTKT +TGSLN+  G V+T+ TAT +  G NF A+++GF
Sbjct: 304 VYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGF 363

Query: 369 ENTAGAAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA 428
           ENTAG   HQAVALRV +D ++FYNCQIDGYQDTLY H+HRQF+RDCT+SGT+DF+FG+ 
Sbjct: 364 ENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDG 423

Query: 429 ATIFQNCKLVVRKPMDNQQCIVTAHGRLERREPTALIFQSCHFMGDPAYLPFKNVNKAYL 488
             + QNC +VVRKPM +Q C++TA GR ++RE T L+ Q+CH  G+PAY+P K++NKAYL
Sbjct: 424 IVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYL 483

Query: 489 GRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNTGPGADESKRVKWRG 548
           GRPWK++SRTII+G+ IDD+I P GWLPW GDF LNTL+YAE +N GPG+++++RVKW G
Sbjct: 484 GRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPG 543

Query: 549 IKHITPQHAADFTPRRFIHGDAWIPAKGIPY 571
           IK ++P+ A  FTP RF+ G+ WIP   +PY
Sbjct: 544 IKKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

BLAST of HG10010367 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 554.3 bits (1427), Expect = 1.2e-157
Identity = 276/575 (48.00%), Postives = 393/575 (68.35%), Query Frame = 0

Query: 8   AEGDKQKKKIAIISVSSLILVAMVVAVTVGVNNAPHEEHDDGHGGETHEISSTTKAIKAI 67
           ++GDK+KK I   SVS  +   +++ V+V V  + H   DD    E H I  TTKA++A+
Sbjct: 3   SDGDKKKKFIVAGSVSGFL---VIMVVSVAVVTSKHSPKDD----ENH-IRKTTKAVQAV 62

Query: 68  CQPTDYKESCEKSLLAEAGNTTDPKELVKVGFKIATRNLNEAI-RNSTTLKELA-KDPRT 127
           C PTD+K++C  SL+  + ++ DP +L+K+GFK+  +++NE++ + S  +K  A K+P  
Sbjct: 63  CAPTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEA 122

Query: 128 NQALQNCHELLEYAIDDLNESFERIGTFQMSKLDEFVGDLKIWLSGALTYEQTCLDGFEN 187
             A + C +L+  AIDDL +  +    F + +++ FV DL++WLSG++ ++QTC+D F  
Sbjct: 123 KGAFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGE 182

Query: 188 TTGDAGVRMQEFLKSAQEMTSNGLAIVNELSSILGSLQIPGMTG------RRLLEEDDEE 247
              +    M +  K+++E++SN LA+V  +S+++ +  + G+TG      R+LL  +D  
Sbjct: 183 IKSNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTED-S 242

Query: 248 MPSWVSDGKRRLMQMQAAG-SSVKPDLVVAQDGSGKYNTINAALADVPLKSNKTFVIYVK 307
           +P+WV    RRLM  Q  G   VK + VVAQDG+G++ TI  AL  VP  +   F+I++K
Sbjct: 243 IPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFIIHIK 302

Query: 308 AGIYKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNF-IDGVQTFKTATFSAIGANFYARDM 367
            GIYKE V + K M H+T  GDGP KT++TGSLNF I  V+TF TAT +  G +F A+++
Sbjct: 303 EGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNI 362

Query: 368 GFENTAGAAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFG 427
           G ENTAG    QAVALRV +D ++F++CQIDG+QDTLY H+HRQFYRDCT+SGT+DF+FG
Sbjct: 363 GIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFG 422

Query: 428 NAATIFQNCKLVVRKPMDNQQCIVTAHGRLERREPTALIFQSCHFMGDPAYLPFKNVNKA 487
           +A  I QNCK+VVRKP   Q C+VTA GR   RE T L+   CH  GDPAY+P K+VNKA
Sbjct: 423 DAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKA 482

Query: 488 YLGRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNTGPGADESKRVKW 547
           YLGRPWK++SRTII+ + IDD+I P GWLPW GDF L TL+YAE  NTGPG+++++RVKW
Sbjct: 483 YLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKW 542

Query: 548 RGIKHITPQHAADFTPRRFIHGDAWIPAKGIPYSS 573
            GIK +TPQ A  +T  RF+ GD WIP   +PY++
Sbjct: 543 PGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

BLAST of HG10010367 vs. TAIR 10
Match: AT4G15980.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 483.8 bits (1244), Expect = 1.9e-136
Identity = 264/703 (37.55%), Postives = 379/703 (53.91%), Query Frame = 0

Query: 16  KIAIISVSSLILVAMVVAVTVGVNNAPHEEHDDGHGGETHEISSTTKAIKAICQPTDYKE 75
           K  ++ V++LI+ AMV+ V     N+P  + ++ H      I+ TT +I  IC  TDYK+
Sbjct: 3   KYVLLGVTALIM-AMVICVEANDGNSPSRKMEEVHRESKLMITKTTVSI--ICASTDYKQ 62

Query: 76  SCEKSLLAEAGNTTDPKELVKVGFKIATRNLNEAI-RNSTTLKELA-KDPRTNQALQNCH 135
            C  SL      + DP+ L++  F +A  ++   I R    LK  A  D  T +AL  C 
Sbjct: 63  DCTTSLATV--RSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTCR 122

Query: 136 ELLEYAIDDLNESFERIGTFQMSKLDEFVGDLKIWLSGALTYEQTCLDGFENTTGDAGVR 195
           EL++ AIDDL ++ ++   F  ++L +FV DL +WLSG++TY+QTC+DGFE    +A V 
Sbjct: 123 ELMDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAVM 182

Query: 196 MQEFLKSAQEMTSNGLAIVNELSSILGSLQIP---------------------------- 255
           M+  ++  Q +TSNGLAI   L  +L + +IP                            
Sbjct: 183 MERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGSSQ 242

Query: 256 -----------------------------------------------------------G 315
                                                                       
Sbjct: 243 DSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKKSA 302

Query: 316 MTGRRLLEE--------------------------------------------------- 375
            +G + L++                                                   
Sbjct: 303 FSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDSLK 362

Query: 376 -----DDEEMPSWVSDGKRRLMQMQAAGSSVKPDLVVAQDGSGKYNTINAALADVPLKSN 435
                ++ E P WV+   RRL+  +   + +K ++VVA+DGSGK  TI  ALA VP+K+ 
Sbjct: 363 DRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVPMKNT 422

Query: 436 KTFVIYVKAGIYKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNFI-DGVQTFKTATFSAIG 495
           K FVI++K G+YKE V + K M H+   GDGPTKT++TG + F+ D V T++TA+ +  G
Sbjct: 423 KKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAVNG 482

Query: 496 ANFYARDMGFENTAGAAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTIS 555
             F A+D+GFENTAGAA+HQAVALRV +D ++F+NC ++GYQDTLY H HRQFYR+C +S
Sbjct: 483 DYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCRVS 542

Query: 556 GTIDFVFGNAATIFQNCKLVVRKPMDNQQCIVTAHGRLERREPTALIFQSCHFMGDPAYL 573
           GTIDFVFG+A  +FQNC+ V+R+PM++QQCIVTA GR +RRE T ++  +    GD +YL
Sbjct: 543 GTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDASYL 602