HG10010356 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10010356
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionchaperone protein dnaJ 49-like
LocationChr06: 21422061 .. 21423119 (-)
RNA-Seq ExpressionHG10010356
SyntenyHG10010356
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGGGAATAAGGACGAAGCTTTAAGATGTATTCGCATGGCGGAAGAAGCAATTGCGTCGGGGAATAAAGAGCGAGCACTTAGATTCATTAAAATTGCTCGTCGCCTTAATCAGAGTTTGCAAGTCGACGAGCTATTGACGGCGTGTGAAGAGATCGGTTCGGGATCACCTTCATCTTCTTCCGATGAGAAACGCGCGGGAAAAGTTGAGAGTGTTTCTGGTTCAGTGAAACATGTTGATGGTTTGAATGGTGAACGTAATTACACTATAGAACACGTTCAATTGATTAGGCAGATAAAAACAACCGAGGACTATTACAGAATTCTTGGTGTGGAGAAAACTAGTTCGGCTGAAGAGATTAAGAGGGCTTATAAGAAACTGTCTTTGAAAGTTCACCCTGATAAAAACAAGGCTCCTGGTTCAGATGAAGCATTCAAGAAATTATCCAAGGCTTTCATGTGTTTGAGCGATGATACTTTGAGAAGGCAATATGATCACACGGGTTTGGTTGATCAGTATGAGTACAACCAACAGCATAATGTAAGGCATAGGAGAAGGAGAACGGGTCATGATTTGTTTGAAGAAAATTTTGATCCTGATGAGATATTCAGAGCGTTCTTTGGGCAGGGAAATATGTTTCAGACGAGTCGTGCTTATACTTATAGAACTGCAGGTGCGGGAAGCCAACAGAGAACAGAATCTGATGGGGGAGGTCCCAGCTTGTTGATTATTCTTCTAATGTTACCATTCTTGTTAATTTGCTTGTTGGCTTATATGCCATTTCCAGAGCCGGACTATGCTTTGCATAAAAACCTATCCTATAGTATTCCTATGACTACGGAGAAACATGGAGTGGAATTTTTTGTTAAATCATCTGATTTTGATGTAAGATATCCTCTTGGAAGCCCAGGTCGAGCTGAAATAGAGAATAGTGTGCTGAGGGATTATAGAAACATGGTTTGGCGTTATTGTCATGCAGAACTCCAGAGACGTCAGTGGAATAGAAATCTCCCAACTCCTCACTGTGAGAAGCTGAATAATCTCGGTGTAGCATAA

mRNA sequence

ATGGATGGGAATAAGGACGAAGCTTTAAGATGTATTCGCATGGCGGAAGAAGCAATTGCGTCGGGGAATAAAGAGCGAGCACTTAGATTCATTAAAATTGCTCGTCGCCTTAATCAGAGTTTGCAAGTCGACGAGCTATTGACGGCGTGTGAAGAGATCGGTTCGGGATCACCTTCATCTTCTTCCGATGAGAAACGCGCGGGAAAAGTTGAGAGTGTTTCTGGTTCAGTGAAACATGTTGATGGTTTGAATGGTGAACGTAATTACACTATAGAACACGTTCAATTGATTAGGCAGATAAAAACAACCGAGGACTATTACAGAATTCTTGGTGTGGAGAAAACTAGTTCGGCTGAAGAGATTAAGAGGGCTTATAAGAAACTGTCTTTGAAAGTTCACCCTGATAAAAACAAGGCTCCTGGTTCAGATGAAGCATTCAAGAAATTATCCAAGGCTTTCATGTGTTTGAGCGATGATACTTTGAGAAGGCAATATGATCACACGGGTTTGGTTGATCAGTATGAGTACAACCAACAGCATAATGTAAGGCATAGGAGAAGGAGAACGGGTCATGATTTGTTTGAAGAAAATTTTGATCCTGATGAGATATTCAGAGCGTTCTTTGGGCAGGGAAATATGTTTCAGACGAGTCGTGCTTATACTTATAGAACTGCAGGTGCGGGAAGCCAACAGAGAACAGAATCTGATGGGGGAGGTCCCAGCTTGTTGATTATTCTTCTAATGTTACCATTCTTGTTAATTTGCTTGTTGGCTTATATGCCATTTCCAGAGCCGGACTATGCTTTGCATAAAAACCTATCCTATAGTATTCCTATGACTACGGAGAAACATGGAGTGGAATTTTTTGTTAAATCATCTGATTTTGATGTAAGATATCCTCTTGGAAGCCCAGGTCGAGCTGAAATAGAGAATAGTGTGCTGAGGGATTATAGAAACATGGTTTGGCGTTATTGTCATGCAGAACTCCAGAGACGTCAGTGGAATAGAAATCTCCCAACTCCTCACTGTGAGAAGCTGAATAATCTCGGTGTAGCATAA

Coding sequence (CDS)

ATGGATGGGAATAAGGACGAAGCTTTAAGATGTATTCGCATGGCGGAAGAAGCAATTGCGTCGGGGAATAAAGAGCGAGCACTTAGATTCATTAAAATTGCTCGTCGCCTTAATCAGAGTTTGCAAGTCGACGAGCTATTGACGGCGTGTGAAGAGATCGGTTCGGGATCACCTTCATCTTCTTCCGATGAGAAACGCGCGGGAAAAGTTGAGAGTGTTTCTGGTTCAGTGAAACATGTTGATGGTTTGAATGGTGAACGTAATTACACTATAGAACACGTTCAATTGATTAGGCAGATAAAAACAACCGAGGACTATTACAGAATTCTTGGTGTGGAGAAAACTAGTTCGGCTGAAGAGATTAAGAGGGCTTATAAGAAACTGTCTTTGAAAGTTCACCCTGATAAAAACAAGGCTCCTGGTTCAGATGAAGCATTCAAGAAATTATCCAAGGCTTTCATGTGTTTGAGCGATGATACTTTGAGAAGGCAATATGATCACACGGGTTTGGTTGATCAGTATGAGTACAACCAACAGCATAATGTAAGGCATAGGAGAAGGAGAACGGGTCATGATTTGTTTGAAGAAAATTTTGATCCTGATGAGATATTCAGAGCGTTCTTTGGGCAGGGAAATATGTTTCAGACGAGTCGTGCTTATACTTATAGAACTGCAGGTGCGGGAAGCCAACAGAGAACAGAATCTGATGGGGGAGGTCCCAGCTTGTTGATTATTCTTCTAATGTTACCATTCTTGTTAATTTGCTTGTTGGCTTATATGCCATTTCCAGAGCCGGACTATGCTTTGCATAAAAACCTATCCTATAGTATTCCTATGACTACGGAGAAACATGGAGTGGAATTTTTTGTTAAATCATCTGATTTTGATGTAAGATATCCTCTTGGAAGCCCAGGTCGAGCTGAAATAGAGAATAGTGTGCTGAGGGATTATAGAAACATGGTTTGGCGTTATTGTCATGCAGAACTCCAGAGACGTCAGTGGAATAGAAATCTCCCAACTCCTCACTGTGAGAAGCTGAATAATCTCGGTGTAGCATAA

Protein sequence

MDGNKDEALRCIRMAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIGSGSPSSSSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTEDYYRILGVEKTSSAEEIKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQHNVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTAGAGSQQRTESDGGGPSLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSIPMTTEKHGVEFFVKSSDFDVRYPLGSPGRAEIENSVLRDYRNMVWRYCHAELQRRQWNRNLPTPHCEKLNNLGVA
Homology
BLAST of HG10010356 vs. NCBI nr
Match: XP_038874732.1 (chaperone protein dnaJ 49 [Benincasa hispida] >XP_038874733.1 chaperone protein dnaJ 49 [Benincasa hispida] >XP_038874734.1 chaperone protein dnaJ 49 [Benincasa hispida])

HSP 1 Score: 675.6 bits (1742), Expect = 2.3e-190
Identity = 337/352 (95.74%), Postives = 343/352 (97.44%), Query Frame = 0

Query: 1   MDGNKDEALRCIRMAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIGSGSPSS 60
           MDGNKDEALRCIR+AEEAIASGNKERALRFIKIARRLNQSLQVDELLT CEEIGSGSPSS
Sbjct: 1   MDGNKDEALRCIRIAEEAIASGNKERALRFIKIARRLNQSLQVDELLTVCEEIGSGSPSS 60

Query: 61  SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTEDYYRILGVEKTSSAEE 120
           S DEKRAGKVES SGSVKHVDGL+GERNY+IEHVQLIRQIKTTEDYYRILGVEKTSSAEE
Sbjct: 61  SFDEKRAGKVESASGSVKHVDGLSGERNYSIEHVQLIRQIKTTEDYYRILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 180
           IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLV QYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVAQYEYNQQH 180

Query: 181 NVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTAGAGSQQRTESDGGGP 240
           NVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSR+YTYRT GA SQQRTES GGGP
Sbjct: 181 NVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRSYTYRTGGARSQQRTESAGGGP 240

Query: 241 SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSIPMTTEKHGVEFFVKSSDFDVRYP 300
           SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSIP+ TEKHGVEFFVKSSDFDVRYP
Sbjct: 241 SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSIPVITEKHGVEFFVKSSDFDVRYP 300

Query: 301 LGSPGRAEIENSVLRDYRNMVWRYCHAELQRRQWNRNLPTPHCEKLNNLGVA 353
           LGSPGRAEIENSVLRDYRNMVWRYCH ELQRRQWN+NLPTPHCEKLNNLGVA
Sbjct: 301 LGSPGRAEIENSVLRDYRNMVWRYCHVELQRRQWNKNLPTPHCEKLNNLGVA 352

BLAST of HG10010356 vs. NCBI nr
Match: XP_016902270.1 (PREDICTED: chaperone protein dnaJ 49-like [Cucumis melo] >XP_016902271.1 PREDICTED: chaperone protein dnaJ 49-like [Cucumis melo] >XP_016902272.1 PREDICTED: chaperone protein dnaJ 49-like [Cucumis melo])

HSP 1 Score: 642.9 bits (1657), Expect = 1.6e-180
Identity = 321/352 (91.19%), Postives = 333/352 (94.60%), Query Frame = 0

Query: 1   MDGNKDEALRCIRMAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIGSGSPSS 60
           MDGNKDEALRCIR+AEE+IASGNKERALRFIKIARRLNQSLQ+DELLTACEE+GSG    
Sbjct: 1   MDGNKDEALRCIRIAEESIASGNKERALRFIKIARRLNQSLQIDELLTACEEMGSG---- 60

Query: 61  SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTEDYYRILGVEKTSSAEE 120
           SS EKRAGK ESVSGSVKHVDGLNGERNY++EHVQLIRQIKTT DYY ILGVEKTSSAEE
Sbjct: 61  SSAEKRAGKGESVSGSVKHVDGLNGERNYSMEHVQLIRQIKTTMDYYGILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 180
           IKRAY+KLSLKVHPDKNKAPGS+EAFKKLSKAF CLSDDTLRR+YD T LVDQYEYNQQH
Sbjct: 121 IKRAYRKLSLKVHPDKNKAPGSEEAFKKLSKAFSCLSDDTLRRRYDQTALVDQYEYNQQH 180

Query: 181 NVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTAGAGSQQRTESDGGGP 240
           NVRHRRRR GHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRT GAGSQQRTESDGGGP
Sbjct: 181 NVRHRRRRNGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGGAGSQQRTESDGGGP 240

Query: 241 SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSIPMTTEKHGVEFFVKSSDFDVRYP 300
           S LIILLMLPFLLICLLAYMPFPEP+YALHKNLSYSIPM TEKHGVEFFVKSSDF+VRYP
Sbjct: 241 SFLIILLMLPFLLICLLAYMPFPEPEYALHKNLSYSIPMATEKHGVEFFVKSSDFNVRYP 300

Query: 301 LGSPGRAEIENSVLRDYRNMVWRYCHAELQRRQWNRNLPTPHCEKLNNLGVA 353
           LGSPGR EIENSVLRDYRNMVWRYCH ELQRRQWN+NLPTPHCEKLN L VA
Sbjct: 301 LGSPGRVEIENSVLRDYRNMVWRYCHIELQRRQWNKNLPTPHCEKLNTLAVA 348

BLAST of HG10010356 vs. NCBI nr
Match: XP_004148209.1 (chaperone protein dnaJ 49 [Cucumis sativus] >XP_011657260.1 chaperone protein dnaJ 49 [Cucumis sativus] >XP_011657262.1 chaperone protein dnaJ 49 [Cucumis sativus] >KGN47372.1 hypothetical protein Csa_022981 [Cucumis sativus])

HSP 1 Score: 634.4 bits (1635), Expect = 5.8e-178
Identity = 317/352 (90.06%), Postives = 331/352 (94.03%), Query Frame = 0

Query: 1   MDGNKDEALRCIRMAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIGSGSPSS 60
           MDGNKDEALRCIR+AEE+IASGNKERALRFIKIARRLNQS+QVDELL ACEEIGSG    
Sbjct: 1   MDGNKDEALRCIRIAEESIASGNKERALRFIKIARRLNQSVQVDELLAACEEIGSG---- 60

Query: 61  SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTEDYYRILGVEKTSSAEE 120
           SS+EKRAGK ESVSGSVKH DGLNGERNY++EHVQLIRQIKTT+DYY ILGVEKTSSAEE
Sbjct: 61  SSEEKRAGKGESVSGSVKHGDGLNGERNYSMEHVQLIRQIKTTKDYYGILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 180
           IKRAY+KLSLKVHPDKNKAPGS+EAFKKLSKAF CLSDDTLRRQYDHT LVDQYEYNQQH
Sbjct: 121 IKRAYRKLSLKVHPDKNKAPGSEEAFKKLSKAFSCLSDDTLRRQYDHTPLVDQYEYNQQH 180

Query: 181 NVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTAGAGSQQRTESDGGGP 240
           NVR RRRR GHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRT GAGSQQRTES GGGP
Sbjct: 181 NVRQRRRRNGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGGAGSQQRTESYGGGP 240

Query: 241 SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSIPMTTEKHGVEFFVKSSDFDVRYP 300
           + LIILLMLPFLLICLLAYMPFPEP+YALHK+LSYSIPM TEKHGVEFFVKSSDFD RYP
Sbjct: 241 NFLIILLMLPFLLICLLAYMPFPEPEYALHKSLSYSIPMATEKHGVEFFVKSSDFDERYP 300

Query: 301 LGSPGRAEIENSVLRDYRNMVWRYCHAELQRRQWNRNLPTPHCEKLNNLGVA 353
           LGSPGR E+ENSVLRDYRNMVWRYCH ELQRRQWN+NLPTPHCEKLN L VA
Sbjct: 301 LGSPGRVELENSVLRDYRNMVWRYCHIELQRRQWNKNLPTPHCEKLNTLAVA 348

BLAST of HG10010356 vs. NCBI nr
Match: KAG6575070.1 (Chaperone protein dnaJ 49, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 615.1 bits (1585), Expect = 3.6e-172
Identity = 308/352 (87.50%), Postives = 327/352 (92.90%), Query Frame = 0

Query: 1   MDGNKDEALRCIRMAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIGSGSPSS 60
           MDGNKDEALRCIR+AEEAIASGNKERAL+FIKIA RLN+SL VDELL+ACEEI   SPSS
Sbjct: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLSACEEIDLKSPSS 60

Query: 61  SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTEDYYRILGVEKTSSAEE 120
           SSD KRAGKV SVSGS  HVDGLNGERNY+IEHVQLIRQIKT +DYY+ILGVE+TSSAEE
Sbjct: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVERTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 180
           IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDT RRQYDHT LVDQYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH 180

Query: 181 NVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTAGAGSQQRTESDGGGP 240
           NV  RRRRTG DLFEENFDPDEIFRAFFGQGNMFQTSRAYTY T GA SQ+RTESDGGGP
Sbjct: 181 NV--RRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGP 240

Query: 241 SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSIPMTTEKHGVEFFVKSSDFDVRYP 300
           +LLI+LLMLPFLLI LLAYMPFPEPDY+LHKNLSYSIPM TEKHGVEFFVKSSDFD RYP
Sbjct: 241 NLLILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYP 300

Query: 301 LGSPGRAEIENSVLRDYRNMVWRYCHAELQRRQWNRNLPTPHCEKLNNLGVA 353
           LGS  R+EIE+SV+RDY+NMVWRYCH ELQRR+WN+NLPTPHCEK+NNLG A
Sbjct: 301 LGSGARSEIESSVIRDYKNMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA 350

BLAST of HG10010356 vs. NCBI nr
Match: XP_022958924.1 (chaperone protein dnaJ 49 [Cucurbita moschata] >XP_022958925.1 chaperone protein dnaJ 49 [Cucurbita moschata])

HSP 1 Score: 614.8 bits (1584), Expect = 4.7e-172
Identity = 307/352 (87.22%), Postives = 329/352 (93.47%), Query Frame = 0

Query: 1   MDGNKDEALRCIRMAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIGSGSPSS 60
           MDGNKDEALRCIR+AEEAIASGNKERAL+FIKIA RLN+SL+V+ELL+ACE+I S SPSS
Sbjct: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLEVNELLSACEDIDSKSPSS 60

Query: 61  SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTEDYYRILGVEKTSSAEE 120
           SSD KRAGKV SVSGS  HVDGLNGERNY+IEHVQLIRQIKT +DYY+ILGVEKTSSAEE
Sbjct: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 180
           IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLS+DT RRQYDHT LVDQYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSNDTSRRQYDHTALVDQYEYNQQH 180

Query: 181 NVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTAGAGSQQRTESDGGGP 240
           NV  RRRRTG DLFEENFDPDEIFRAFFGQGNMFQTSRAYTY T GA SQ+RTESDGGGP
Sbjct: 181 NV--RRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGP 240

Query: 241 SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSIPMTTEKHGVEFFVKSSDFDVRYP 300
           +LLI+LLMLPFLLI LLAYMPFPEPDY+LHKNLSYSIPM TEKHGVEFFVKSSDFD RYP
Sbjct: 241 NLLILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYP 300

Query: 301 LGSPGRAEIENSVLRDYRNMVWRYCHAELQRRQWNRNLPTPHCEKLNNLGVA 353
           LGS  R+EIE+SV+RDY+NMVWRYCH ELQRR+WN+NLPTPHCEK+NNLG A
Sbjct: 301 LGSGARSEIESSVIRDYKNMVWRYCHIELQRRKWNKNLPTPHCEKMNNLGFA 350

BLAST of HG10010356 vs. ExPASy Swiss-Prot
Match: Q9FH28 (Chaperone protein dnaJ 49 OS=Arabidopsis thaliana OX=3702 GN=ATJ49 PE=2 SV=2)

HSP 1 Score: 346.3 bits (887), Expect = 4.1e-94
Identity = 184/354 (51.98%), Postives = 254/354 (71.75%), Query Frame = 0

Query: 1   MDGNKDEALRCIRMAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIGSGSPSS 60
           MDGNKD+A RC+R+AE+AI SG+KERAL+FI +A+RLN SL VDEL+ AC+ + S S +S
Sbjct: 1   MDGNKDDASRCLRIAEDAIVSGDKERALKFINMAKRLNPSLSVDELVAACDNLDSVSRNS 60

Query: 61  SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTEDYYRILGVEKTSSAEE 120
           S  E    K++++ G    ++   G+  YT E+V L+R I    DYY ILG+EK  S +E
Sbjct: 61  SVSE----KLKTMDGDDDKLE--TGKMKYTEENVDLVRNIIRNNDYYAILGLEKNCSVDE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 180
           I++AY+KLSLKVHPDKNKAPGS+EAFKK+SKAF CLSD   RRQ+D  G+VD++++ Q+ 
Sbjct: 121 IRKAYRKLSLKVHPDKNKAPGSEEAFKKVSKAFTCLSDGNSRRQFDQVGIVDEFDHVQRR 180

Query: 181 NVRHRRR-RTGHDLFEENFDPDEIFRAFFG-QGNMFQTSRAYTYRTAGAGSQQRTES-DG 240
           N R RRR  T +D F++ FDP+EIFR  FG Q  +F+ S A  YRT    +Q R E  + 
Sbjct: 181 NRRPRRRYNTRNDFFDDEFDPEEIFRTVFGQQREVFRASHA--YRTRQPRNQFREEEINV 240

Query: 241 GGPSLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSIPMTTEKHGVEFFVKS-SDFD 300
            GPS L I+ +LPF L+ LLAY+PF EPDY+LHKN SY IP TT+   + F+V+S S FD
Sbjct: 241 AGPSCLTIIQILPFFLLLLLAYLPFSEPDYSLHKNQSYQIPKTTQNTEISFYVRSASAFD 300

Query: 301 VRYPLGSPGRAEIENSVLRDYRNMVWRYCHAELQRRQWNRNLPTPHCEKLNNLG 351
            ++PL S  RA +E +V+++Y++ +++ C  ELQ+R+WN+ +PTPHC +L + G
Sbjct: 301 EKFPLSSSARANLEGNVIKEYKHFLFQSCRIELQKRRWNKKIPTPHCIELQDRG 346

BLAST of HG10010356 vs. ExPASy Swiss-Prot
Match: Q7ZXQ8 (DnaJ homolog subfamily B member 14 OS=Xenopus laevis OX=8355 GN=dnajb14 PE=2 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 7.9e-37
Identity = 112/348 (32.18%), Postives = 178/348 (51.15%), Query Frame = 0

Query: 1   MDGNKDEALRCIRMAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIGSGSPSS 60
           M+ N+DEA RC+R+ + AI +G+KE+A RF   A RL  S +   LL A E+  + +  +
Sbjct: 1   MESNRDEAERCVRIGKAAIEAGDKEKARRFFSKAERLYPSSEARVLLDALEK--NDTAGN 60

Query: 61  SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTEDYYRILGVEKTSSAEE 120
               ++  K      + K   G  G + +T + V  +++IK  + YY +LGV   +  E+
Sbjct: 61  GPQSEKMSKSTEQPKAEKDSSGDTG-KGHTQDQVDGVQRIKKCKTYYEVLGVSPDAGEED 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 180
           +K+AY+KL+LK HPDKN APG+ EAFKK+  A+  LS+   R+QYD TG  D        
Sbjct: 121 LKKAYRKLALKFHPDKNHAPGATEAFKKIGNAYAVLSNPEKRKQYDLTGSED-------- 180

Query: 181 NVRHRRRRTGHDL---FEENFDPDEIFRAFFGQGNMFQTSRAYTYRTA-----------G 240
           NV++  R  G D    FE +  P+++F  FFG G  F +   +T+               
Sbjct: 181 NVQNNHRNGGFDYHRGFEADITPEDLFNMFFGGG--FPSGSVHTFSNGRTRYSHHQHHHH 240

Query: 241 AGSQQRTESDGGGPSLLIILL-MLPFLLICLLAYMPFPEPDYALHKNLSYSIPMTTEKHG 300
           +G  +  E   GG S+ I L+ ++  +L+ LL+ +    P Y+L+     +I   TE   
Sbjct: 241 SGHDREEERADGGFSMFIQLMPIIVLILVSLLSQLMVSNPPYSLYPRSGQTIKRVTENLQ 300

Query: 301 VEFFVKSSDFDVRYPLGSPGRAEIENSVLRDYRNMVWRYCHAELQRRQ 334
           + ++V S DF   Y        ++E ++  DY   V   C  E Q++Q
Sbjct: 301 ISYYV-SKDFKSEY--NGMLLQKLEKNIEEDYVANVRNNCWRERQQKQ 332

BLAST of HG10010356 vs. ExPASy Swiss-Prot
Match: Q28I38 (DnaJ homolog subfamily B member 14 OS=Xenopus tropicalis OX=8364 GN=dnajb14 PE=2 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 1.0e-36
Identity = 116/344 (33.72%), Postives = 170/344 (49.42%), Query Frame = 0

Query: 1   MDGNKDEALRCIRMAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIGSGSPSS 60
           M+ N+DEA RC+R+A+ AI +G+KE+A RF+  A RL  S +   LL A E+  +     
Sbjct: 1   MESNRDEAERCVRIAKAAIEAGDKEKAKRFLSKAERLYPSSEARALLQAFEKNDTAGNGP 60

Query: 61  SSDEKRAG----KVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTEDYYRILGVEKTS 120
            S +   G    K E  S +    D   G     ++ VQ I++ KT   YY +LGV   +
Sbjct: 61  QSAKMAKGTEQPKAEKDSNASASSDTGKGHTQDQLDGVQRIKKCKT---YYEVLGVSTDA 120

Query: 121 SAEEIKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEY 180
             E++K+AY+KL+LK HPDKN APG+ EAFKK+  A+  LS+   R+QYD TG  DQ + 
Sbjct: 121 GEEDLKKAYRKLALKFHPDKNHAPGATEAFKKIGNAYAVLSNPEKRKQYDLTGSEDQMQN 180

Query: 181 NQQHNVRHRRRRTGHDL---FEENFDPDEIFRAFFGQGNMFQTSRAYTYRTAGA------ 240
           N         R  G D    FE +  P+++F  FFG G  F +   +T+    A      
Sbjct: 181 N--------HRNGGFDYHRGFEADITPEDLFNMFFGGG--FPSGSVHTFSNGRARYSHHQ 240

Query: 241 -----GSQQRTESDGGGPSLLIILL-MLPFLLICLLAYMPFPEPDYALHKNLSYSIPMTT 300
                G  +  E   GG S+ I L+ ++  +L+ LL+      P Y+L+     +    T
Sbjct: 241 HHHHSGHDREDERADGGFSMFIQLMPIIVLILVSLLSQFMVSNPPYSLYPRSGQATKRVT 300

Query: 301 EKHGVEFFVKSSDFDVRYP--LGSPGRAEIENSVLRDYRNMVWR 324
           E   + ++V S DF   Y   L       IE   + + RN  WR
Sbjct: 301 ENLQIAYYV-SKDFQSEYSGILLQKLEKNIEEDYVANVRNNCWR 330

BLAST of HG10010356 vs. ExPASy Swiss-Prot
Match: Q149L6 (DnaJ homolog subfamily B member 14 OS=Mus musculus OX=10090 GN=Dnajb14 PE=2 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 1.3e-36
Identity = 116/348 (33.33%), Postives = 186/348 (53.45%), Query Frame = 0

Query: 1   MDGNKDEALRCIRMAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIGSGSPSS 60
           M+GN+DEA +C+++A EA+++GN+++A RF++ A +L        LL    + GS + SS
Sbjct: 1   MEGNRDEAEKCVQIAREALSAGNRDKAQRFLQKAEKLYPLPAARALLEIIMKNGSTAGSS 60

Query: 61  SSDEKRAGKVESVSGSVKHVDGLNGE----RNYTIEHVQLIRQIKTTEDYYRILGVEKTS 120
           +   K  G  +    S    DG +G     + YT + V+ +  I   ++YY +LGV K +
Sbjct: 61  THCRKPPGSSDQSKPSCGK-DGTSGAGEGGKVYTKDQVEGVLSINKCKNYYEVLGVTKDA 120

Query: 121 SAEEIKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEY 180
             E++K+AY+KL+LK HPDKN APG+ +AFKK+  A+  LS+   R+QYD TG  +Q   
Sbjct: 121 GDEDLKKAYRKLALKFHPDKNHAPGATDAFKKIGNAYAVLSNPEKRKQYDLTGSEEQ-AC 180

Query: 181 NQQHNVRHRRRRTGHDLFEENFDPDEIFRAFFGQG------NMFQTSRA---YTYRTAGA 240
           N Q+N R    R      E +  P+++F  FFG G      + F   RA   + ++   +
Sbjct: 181 NHQNNGRFNFHRG----CEADITPEDLFNIFFGGGFPSGSVHSFSNGRAAYSHQHQHRHS 240

Query: 241 GSQQRTESDGGGPSLLIILL-MLPFLLICLLAYMPFPEPDYALH--KNLSYSIPMTTEKH 300
           G ++  E   GG S+ I L+ ++  +L+ LL+ +    P Y+L+       +I M TE  
Sbjct: 241 GHEREEERADGGFSVFIQLMPIIVLILVSLLSQLMVSNPPYSLYPRSGSGQTIKMQTENL 300

Query: 301 GVEFFVKSSDFDVRYPLGSPGRAEIENSVLRDYRNMVWRYCHAELQRR 333
           GV ++V S DF   Y        ++E SV  DY   +   C  E Q++
Sbjct: 301 GVVYYV-SKDFKSEYK--GTLLQKVEKSVEEDYVTNIRNNCWKERQQK 339

BLAST of HG10010356 vs. ExPASy Swiss-Prot
Match: Q8TBM8 (DnaJ homolog subfamily B member 14 OS=Homo sapiens OX=9606 GN=DNAJB14 PE=1 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.1e-35
Identity = 114/355 (32.11%), Postives = 182/355 (51.27%), Query Frame = 0

Query: 1   MDGNKDEALRCIRMAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIGSGS--- 60
           M+GN+DEA +C+ +A EA+ +GN+E+A RF++ A +L        LL    + GS +   
Sbjct: 1   MEGNRDEAEKCVEIAREALNAGNREKAQRFLQKAEKLYPLPSARALLEIIMKNGSTAGNS 60

Query: 61  -----PSSSSDEKRAG-KVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTEDYYRILG 120
                PS S D+ +     +S SGS +      G + YT + V  +  I   ++YY +LG
Sbjct: 61  PHCRKPSGSGDQSKPNCTKDSTSGSGE------GGKGYTKDQVDGVLSINKCKNYYEVLG 120

Query: 121 VEKTSSAEEIKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLV 180
           V K +  E++K+AY+KL+LK HPDKN APG+ +AFKK+  A+  LS+   R+QYD TG  
Sbjct: 121 VTKDAGDEDLKKAYRKLALKFHPDKNHAPGATDAFKKIGNAYAVLSNPEKRKQYDLTGNE 180

Query: 181 DQYEYNQQHNVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTAGAGSQQ 240
           +Q   N Q+N R    R      E +  P+++F  FFG G  F +   +++    AG  Q
Sbjct: 181 EQ-ACNHQNNGRFNFHRG----CEADITPEDLFNIFFGGG--FPSGSVHSFSNGRAGYSQ 240

Query: 241 RTE------------SDGGGPSLLIILLMLPFLLICLLAYMPFPEPDYALH--KNLSYSI 300
           + +             DGG    + ++ ++  +L+ LL+ +    P Y+L+       +I
Sbjct: 241 QHQHRHSGHEREEERGDGGFSVFIQLMPIIVLILVSLLSQLMVSNPPYSLYPRSGTGQTI 300

Query: 301 PMTTEKHGVEFFVKSSDFDVRYPLGSPGRAEIENSVLRDYRNMVWRYCHAELQRR 333
            M TE  GV ++V + DF   Y        ++E SV  DY   +   C  E Q++
Sbjct: 301 KMQTENLGVVYYV-NKDFKNEYK--GMLLQKVEKSVEEDYVTNIRNNCWKERQQK 339

BLAST of HG10010356 vs. ExPASy TrEMBL
Match: A0A1S4E2R1 (chaperone protein dnaJ 49-like OS=Cucumis melo OX=3656 GN=LOC103497850 PE=4 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 7.9e-181
Identity = 321/352 (91.19%), Postives = 333/352 (94.60%), Query Frame = 0

Query: 1   MDGNKDEALRCIRMAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIGSGSPSS 60
           MDGNKDEALRCIR+AEE+IASGNKERALRFIKIARRLNQSLQ+DELLTACEE+GSG    
Sbjct: 1   MDGNKDEALRCIRIAEESIASGNKERALRFIKIARRLNQSLQIDELLTACEEMGSG---- 60

Query: 61  SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTEDYYRILGVEKTSSAEE 120
           SS EKRAGK ESVSGSVKHVDGLNGERNY++EHVQLIRQIKTT DYY ILGVEKTSSAEE
Sbjct: 61  SSAEKRAGKGESVSGSVKHVDGLNGERNYSMEHVQLIRQIKTTMDYYGILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 180
           IKRAY+KLSLKVHPDKNKAPGS+EAFKKLSKAF CLSDDTLRR+YD T LVDQYEYNQQH
Sbjct: 121 IKRAYRKLSLKVHPDKNKAPGSEEAFKKLSKAFSCLSDDTLRRRYDQTALVDQYEYNQQH 180

Query: 181 NVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTAGAGSQQRTESDGGGP 240
           NVRHRRRR GHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRT GAGSQQRTESDGGGP
Sbjct: 181 NVRHRRRRNGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGGAGSQQRTESDGGGP 240

Query: 241 SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSIPMTTEKHGVEFFVKSSDFDVRYP 300
           S LIILLMLPFLLICLLAYMPFPEP+YALHKNLSYSIPM TEKHGVEFFVKSSDF+VRYP
Sbjct: 241 SFLIILLMLPFLLICLLAYMPFPEPEYALHKNLSYSIPMATEKHGVEFFVKSSDFNVRYP 300

Query: 301 LGSPGRAEIENSVLRDYRNMVWRYCHAELQRRQWNRNLPTPHCEKLNNLGVA 353
           LGSPGR EIENSVLRDYRNMVWRYCH ELQRRQWN+NLPTPHCEKLN L VA
Sbjct: 301 LGSPGRVEIENSVLRDYRNMVWRYCHIELQRRQWNKNLPTPHCEKLNTLAVA 348

BLAST of HG10010356 vs. ExPASy TrEMBL
Match: A0A0A0KG46 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G306340 PE=4 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 2.8e-178
Identity = 317/352 (90.06%), Postives = 331/352 (94.03%), Query Frame = 0

Query: 1   MDGNKDEALRCIRMAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIGSGSPSS 60
           MDGNKDEALRCIR+AEE+IASGNKERALRFIKIARRLNQS+QVDELL ACEEIGSG    
Sbjct: 1   MDGNKDEALRCIRIAEESIASGNKERALRFIKIARRLNQSVQVDELLAACEEIGSG---- 60

Query: 61  SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTEDYYRILGVEKTSSAEE 120
           SS+EKRAGK ESVSGSVKH DGLNGERNY++EHVQLIRQIKTT+DYY ILGVEKTSSAEE
Sbjct: 61  SSEEKRAGKGESVSGSVKHGDGLNGERNYSMEHVQLIRQIKTTKDYYGILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 180
           IKRAY+KLSLKVHPDKNKAPGS+EAFKKLSKAF CLSDDTLRRQYDHT LVDQYEYNQQH
Sbjct: 121 IKRAYRKLSLKVHPDKNKAPGSEEAFKKLSKAFSCLSDDTLRRQYDHTPLVDQYEYNQQH 180

Query: 181 NVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTAGAGSQQRTESDGGGP 240
           NVR RRRR GHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRT GAGSQQRTES GGGP
Sbjct: 181 NVRQRRRRNGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGGAGSQQRTESYGGGP 240

Query: 241 SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSIPMTTEKHGVEFFVKSSDFDVRYP 300
           + LIILLMLPFLLICLLAYMPFPEP+YALHK+LSYSIPM TEKHGVEFFVKSSDFD RYP
Sbjct: 241 NFLIILLMLPFLLICLLAYMPFPEPEYALHKSLSYSIPMATEKHGVEFFVKSSDFDERYP 300

Query: 301 LGSPGRAEIENSVLRDYRNMVWRYCHAELQRRQWNRNLPTPHCEKLNNLGVA 353
           LGSPGR E+ENSVLRDYRNMVWRYCH ELQRRQWN+NLPTPHCEKLN L VA
Sbjct: 301 LGSPGRVELENSVLRDYRNMVWRYCHIELQRRQWNKNLPTPHCEKLNTLAVA 348

BLAST of HG10010356 vs. ExPASy TrEMBL
Match: A0A6J1H4U8 (chaperone protein dnaJ 49 OS=Cucurbita moschata OX=3662 GN=LOC111460072 PE=4 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 2.3e-172
Identity = 307/352 (87.22%), Postives = 329/352 (93.47%), Query Frame = 0

Query: 1   MDGNKDEALRCIRMAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIGSGSPSS 60
           MDGNKDEALRCIR+AEEAIASGNKERAL+FIKIA RLN+SL+V+ELL+ACE+I S SPSS
Sbjct: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLEVNELLSACEDIDSKSPSS 60

Query: 61  SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTEDYYRILGVEKTSSAEE 120
           SSD KRAGKV SVSGS  HVDGLNGERNY+IEHVQLIRQIKT +DYY+ILGVEKTSSAEE
Sbjct: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 180
           IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLS+DT RRQYDHT LVDQYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSNDTSRRQYDHTALVDQYEYNQQH 180

Query: 181 NVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTAGAGSQQRTESDGGGP 240
           NV  RRRRTG DLFEENFDPDEIFRAFFGQGNMFQTSRAYTY T GA SQ+RTESDGGGP
Sbjct: 181 NV--RRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGP 240

Query: 241 SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSIPMTTEKHGVEFFVKSSDFDVRYP 300
           +LLI+LLMLPFLLI LLAYMPFPEPDY+LHKNLSYSIPM TEKHGVEFFVKSSDFD RYP
Sbjct: 241 NLLILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYP 300

Query: 301 LGSPGRAEIENSVLRDYRNMVWRYCHAELQRRQWNRNLPTPHCEKLNNLGVA 353
           LGS  R+EIE+SV+RDY+NMVWRYCH ELQRR+WN+NLPTPHCEK+NNLG A
Sbjct: 301 LGSGARSEIESSVIRDYKNMVWRYCHIELQRRKWNKNLPTPHCEKMNNLGFA 350

BLAST of HG10010356 vs. ExPASy TrEMBL
Match: A0A6J1KXF3 (chaperone protein dnaJ 49 OS=Cucurbita maxima OX=3661 GN=LOC111499080 PE=4 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 2.5e-171
Identity = 305/352 (86.65%), Postives = 327/352 (92.90%), Query Frame = 0

Query: 1   MDGNKDEALRCIRMAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIGSGSPSS 60
           MDGNKDEALRCIR+AEEAIASGNKERAL+FIKIA RLN+SL+VDELL+ACEEI S SPSS
Sbjct: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLEVDELLSACEEIDSKSPSS 60

Query: 61  SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTEDYYRILGVEKTSSAEE 120
           SSD KR GKV SVSGS  HVDGLNGERNY+IE+VQLIRQIKT +DYY+ILGVEKTSSAEE
Sbjct: 61  SSDGKRTGKVHSVSGSANHVDGLNGERNYSIENVQLIRQIKTAKDYYKILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 180
           IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDT RRQYDHT LVDQYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH 180

Query: 181 NVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTAGAGSQQRTESDGGGP 240
           NV  RRRRTG DLFEENFDPDEIFRAFFGQGNMFQTSRAYTY T GA SQ+RTESDGGGP
Sbjct: 181 NV--RRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGP 240

Query: 241 SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSIPMTTEKHGVEFFVKSSDFDVRYP 300
           +LL++LLMLPFLLI LLAYMPFPEPDY+LHKNLSY IPM TEKHGVEFFVKSSDFD RYP
Sbjct: 241 NLLVLLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYIIPMATEKHGVEFFVKSSDFDERYP 300

Query: 301 LGSPGRAEIENSVLRDYRNMVWRYCHAELQRRQWNRNLPTPHCEKLNNLGVA 353
           LGS  R+EIE+SV+RDY++MVWRYCH ELQRR+WN+NLPTPHCEK+NNLG A
Sbjct: 301 LGSRARSEIESSVIRDYKSMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA 350

BLAST of HG10010356 vs. ExPASy TrEMBL
Match: A0A6J1CB72 (chaperone protein dnaJ 49 OS=Momordica charantia OX=3673 GN=LOC111009903 PE=4 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 1.6e-170
Identity = 302/348 (86.78%), Postives = 324/348 (93.10%), Query Frame = 0

Query: 1   MDGNKDEALRCIRMAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIGSGSPSS 60
           MDGNKDEALRCI +AE AIASGNK RALRFIKIARRLN+SLQVDELL ACEE+ SGSP+S
Sbjct: 1   MDGNKDEALRCIHIAEAAIASGNKARALRFIKIARRLNRSLQVDELLKACEEVDSGSPTS 60

Query: 61  SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTEDYYRILGVEKTSSAEE 120
           SSDEKRAGKVESV GS KHV+GLNGERNYT EHV+LIRQI TT+DYYRILGVEKTSSAEE
Sbjct: 61  SSDEKRAGKVESVPGSAKHVEGLNGERNYTTEHVRLIRQINTTKDYYRILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 180
           IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDT RRQYDHT LVDQYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTDLVDQYEYNQQH 180

Query: 181 NVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMF-QTSRAYTYRTAGAGSQQRTESDGGG 240
           NVR RRRRTGHDLFEENFDP+EIFRAFFGQGNMF  +SRAYTYRT G GSQQR ES+GGG
Sbjct: 181 NVRQRRRRTGHDLFEENFDPEEIFRAFFGQGNMFHSSSRAYTYRTGGGGSQQRAESNGGG 240

Query: 241 PSLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSIPMTTEKHGVEFFVKSSDFDVRY 300
           P+LLI+LLMLPFLLI LLAY+PFPEPDY+LH NLSY+IPM TEKHGVEF+VKSSDFDV+Y
Sbjct: 241 PNLLILLLMLPFLLIFLLAYLPFPEPDYSLHTNLSYTIPMATEKHGVEFYVKSSDFDVKY 300

Query: 301 PLGSPGRAEIENSVLRDYRNMVWRYCHAELQRRQWNRNLPTPHCEKLN 348
           P GS  RAEIEN+VLRDY++MVWRYCH ELQRRQWN+NLPTPHCEK+N
Sbjct: 301 PPGSLARAEIENTVLRDYKSMVWRYCHIELQRRQWNKNLPTPHCEKMN 348

BLAST of HG10010356 vs. TAIR 10
Match: AT5G49060.1 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 346.3 bits (887), Expect = 2.9e-95
Identity = 184/354 (51.98%), Postives = 254/354 (71.75%), Query Frame = 0

Query: 1   MDGNKDEALRCIRMAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIGSGSPSS 60
           MDGNKD+A RC+R+AE+AI SG+KERAL+FI +A+RLN SL VDEL+ AC+ + S S +S
Sbjct: 1   MDGNKDDASRCLRIAEDAIVSGDKERALKFINMAKRLNPSLSVDELVAACDNLDSVSRNS 60

Query: 61  SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTEDYYRILGVEKTSSAEE 120
           S  E    K++++ G    ++   G+  YT E+V L+R I    DYY ILG+EK  S +E
Sbjct: 61  SVSE----KLKTMDGDDDKLE--TGKMKYTEENVDLVRNIIRNNDYYAILGLEKNCSVDE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 180
           I++AY+KLSLKVHPDKNKAPGS+EAFKK+SKAF CLSD   RRQ+D  G+VD++++ Q+ 
Sbjct: 121 IRKAYRKLSLKVHPDKNKAPGSEEAFKKVSKAFTCLSDGNSRRQFDQVGIVDEFDHVQRR 180

Query: 181 NVRHRRR-RTGHDLFEENFDPDEIFRAFFG-QGNMFQTSRAYTYRTAGAGSQQRTES-DG 240
           N R RRR  T +D F++ FDP+EIFR  FG Q  +F+ S A  YRT    +Q R E  + 
Sbjct: 181 NRRPRRRYNTRNDFFDDEFDPEEIFRTVFGQQREVFRASHA--YRTRQPRNQFREEEINV 240

Query: 241 GGPSLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSIPMTTEKHGVEFFVKS-SDFD 300
            GPS L I+ +LPF L+ LLAY+PF EPDY+LHKN SY IP TT+   + F+V+S S FD
Sbjct: 241 AGPSCLTIIQILPFFLLLLLAYLPFSEPDYSLHKNQSYQIPKTTQNTEISFYVRSASAFD 300

Query: 301 VRYPLGSPGRAEIENSVLRDYRNMVWRYCHAELQRRQWNRNLPTPHCEKLNNLG 351
            ++PL S  RA +E +V+++Y++ +++ C  ELQ+R+WN+ +PTPHC +L + G
Sbjct: 301 EKFPLSSSARANLEGNVIKEYKHFLFQSCRIELQKRRWNKKIPTPHCIELQDRG 346

BLAST of HG10010356 vs. TAIR 10
Match: AT3G57340.1 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 242.7 bits (618), Expect = 4.6e-64
Identity = 142/369 (38.48%), Postives = 218/369 (59.08%), Query Frame = 0

Query: 1   MDGNKDEALRCIRMAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIGSGSPSS 60
           MDGNKD+AL+C+++ + A+ +G++ RAL+F+  ARRL+ +L +D+L++      S  P S
Sbjct: 1   MDGNKDDALKCLKICKSAMEAGDRPRALKFLAKARRLDPNLPIDDLVSELNNNKSDEPGS 60

Query: 61  SSDEKRAGKVESVSGSVK--------HVDGLNGERNYTIEHVQLIRQIKTTEDYYRILGV 120
           +     A   +S + S +             +   +YT E + ++R+IK+ +DYY ILG+
Sbjct: 61  AKSPGSAAAKDSSNSSDRPSLRQRGSSTTSSSSSMSYTEEQISIVRKIKSKKDYYEILGL 120

Query: 121 EKTSSAEEIKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVD 180
           E   S +++++AY+KLSLKVHPDKN+APGS+EAFK +SKAF CLS+D  R++YD +G  D
Sbjct: 121 ESNCSVDDVRKAYRKLSLKVHPDKNQAPGSEEAFKSVSKAFQCLSNDEARKKYDVSG-SD 180

Query: 181 QYEYNQQHNVRHRRRRTGHDLFEENFDPDEIFRAF-----FGQGNMFQTSRAYTYRTAGA 240
           +  Y  + + R      G+  +E+ FDP+EIFR+F     FG G M   +  +     GA
Sbjct: 181 EPIYQPRRSARSNGFNGGY-YYEDEFDPNEIFRSFFGGGGFGGGGMPPATAQFRSFNFGA 240

Query: 241 GSQQRTESDGGGP----SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSIPMTTEK 300
            ++QRT ++   P    +  I+L +LP + I LL +MP  +P Y L     Y    TT+K
Sbjct: 241 -TRQRTANNNQAPDAGFNARILLQLLPVVFILLLNFMPSSQPVYQLSATYPYQYKFTTQK 300

Query: 301 HGVEFFVKSSDFDVRYPLGSPGRAEIENSVLRDYRNMVWRYCHAELQRRQWNRNLPTPHC 353
            GV +FVKSS F+  YP  S  R  +E  V RDY +++ + C  E+QR+QW     TPHC
Sbjct: 301 -GVNYFVKSSKFEQDYPRDSNDRHTLEEQVERDYVSILSQNCRYEMQRKQWGFVRETPHC 360

BLAST of HG10010356 vs. TAIR 10
Match: AT3G57340.2 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 242.7 bits (618), Expect = 4.6e-64
Identity = 142/369 (38.48%), Postives = 218/369 (59.08%), Query Frame = 0

Query: 1   MDGNKDEALRCIRMAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIGSGSPSS 60
           MDGNKD+AL+C+++ + A+ +G++ RAL+F+  ARRL+ +L +D+L++      S  P S
Sbjct: 1   MDGNKDDALKCLKICKSAMEAGDRPRALKFLAKARRLDPNLPIDDLVSELNNNKSDEPGS 60

Query: 61  SSDEKRAGKVESVSGSVK--------HVDGLNGERNYTIEHVQLIRQIKTTEDYYRILGV 120
           +     A   +S + S +             +   +YT E + ++R+IK+ +DYY ILG+
Sbjct: 61  AKSPGSAAAKDSSNSSDRPSLRQRGSSTTSSSSSMSYTEEQISIVRKIKSKKDYYEILGL 120

Query: 121 EKTSSAEEIKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVD 180
           E   S +++++AY+KLSLKVHPDKN+APGS+EAFK +SKAF CLS+D  R++YD +G  D
Sbjct: 121 ESNCSVDDVRKAYRKLSLKVHPDKNQAPGSEEAFKSVSKAFQCLSNDEARKKYDVSG-SD 180

Query: 181 QYEYNQQHNVRHRRRRTGHDLFEENFDPDEIFRAF-----FGQGNMFQTSRAYTYRTAGA 240
           +  Y  + + R      G+  +E+ FDP+EIFR+F     FG G M   +  +     GA
Sbjct: 181 EPIYQPRRSARSNGFNGGY-YYEDEFDPNEIFRSFFGGGGFGGGGMPPATAQFRSFNFGA 240

Query: 241 GSQQRTESDGGGP----SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSIPMTTEK 300
            ++QRT ++   P    +  I+L +LP + I LL +MP  +P Y L     Y    TT+K
Sbjct: 241 -TRQRTANNNQAPDAGFNARILLQLLPVVFILLLNFMPSSQPVYQLSATYPYQYKFTTQK 300

Query: 301 HGVEFFVKSSDFDVRYPLGSPGRAEIENSVLRDYRNMVWRYCHAELQRRQWNRNLPTPHC 353
            GV +FVKSS F+  YP  S  R  +E  V RDY +++ + C  E+QR+QW     TPHC
Sbjct: 301 -GVNYFVKSSKFEQDYPRDSNDRHTLEEQVERDYVSILSQNCRYEMQRKQWGFVRETPHC 360

BLAST of HG10010356 vs. TAIR 10
Match: AT5G05750.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 193.0 bits (489), Expect = 4.1e-49
Identity = 109/285 (38.25%), Postives = 175/285 (61.40%), Query Frame = 0

Query: 1   MDGNKDEALRCIRMAEEAIASGNKERALRFIKIARRLNQSLQVDELLT---------ACE 60
           MDGNKD+AL+C+++ ++AI +G++ RAL+F++ ARRL+ +L +D L++         A E
Sbjct: 1   MDGNKDDALKCLKIGKDAIEAGDRSRALKFLEKARRLDPNLPIDGLVSDLKKQSDEPAAE 60

Query: 61  EIGSGSPSSSSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTEDYYRILG 120
           E   GS ++ S +          GS     G +   + T E   ++R+IK+ +DYY ILG
Sbjct: 61  EDSPGSAANESSKPSDRPSLRQRGSSSSAAGSSSSSSSTEEQRTIVREIKSKKDYYEILG 120

Query: 121 VEKTSSAEEIKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLV 180
           ++   S E+++++Y+KLSLKVHPDKNKAPGS+EAFK +SKAF CLS++  RR+YD +G  
Sbjct: 121 LKSNCSVEDLRKSYRKLSLKVHPDKNKAPGSEEAFKSVSKAFQCLSNEDTRRKYDGSG-S 180

Query: 181 DQYEYNQQHNVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTAGAGSQQ 240
           D+  Y  + + R      G   +++ FD DEIFR+FFG G M   +  +     G G++ 
Sbjct: 181 DEPAYQPRRDARRNNGFNG--FYDDEFDADEIFRSFFGGGEMNPATTQFRSFNFGGGTRT 240

Query: 241 RTESDGGGPSLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYS 277
             ++   G +  ++L +LP + I LL ++P P+P Y+L   ++ S
Sbjct: 241 ANQASDTGFNPRVLLQILPVVFILLLNFLPSPQPIYSLSHRITTS 282

BLAST of HG10010356 vs. TAIR 10
Match: AT2G22360.1 (DNAJ heat shock family protein )

HSP 1 Score: 72.4 bits (176), Expect = 8.2e-13
Identity = 33/70 (47.14%), Postives = 46/70 (65.71%), Query Frame = 0

Query: 100 IKTTEDYYRILGVEKTSSAEEIKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDD 159
           ++   DYY +LGV K ++  EIK AY+KL+   HPD NK PG++E FK++S A+  LSDD
Sbjct: 81  VRADADYYSVLGVSKNATKAEIKSAYRKLARNYHPDVNKDPGAEEKFKEISNAYEVLSDD 140

Query: 160 TLRRQYDHTG 170
             +  YD  G
Sbjct: 141 EKKSLYDRYG 150

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038874732.12.3e-19095.74chaperone protein dnaJ 49 [Benincasa hispida] >XP_038874733.1 chaperone protein ... [more]
XP_016902270.11.6e-18091.19PREDICTED: chaperone protein dnaJ 49-like [Cucumis melo] >XP_016902271.1 PREDICT... [more]
XP_004148209.15.8e-17890.06chaperone protein dnaJ 49 [Cucumis sativus] >XP_011657260.1 chaperone protein dn... [more]
KAG6575070.13.6e-17287.50Chaperone protein dnaJ 49, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022958924.14.7e-17287.22chaperone protein dnaJ 49 [Cucurbita moschata] >XP_022958925.1 chaperone protein... [more]
Match NameE-valueIdentityDescription
Q9FH284.1e-9451.98Chaperone protein dnaJ 49 OS=Arabidopsis thaliana OX=3702 GN=ATJ49 PE=2 SV=2[more]
Q7ZXQ87.9e-3732.18DnaJ homolog subfamily B member 14 OS=Xenopus laevis OX=8355 GN=dnajb14 PE=2 SV=... [more]
Q28I381.0e-3633.72DnaJ homolog subfamily B member 14 OS=Xenopus tropicalis OX=8364 GN=dnajb14 PE=2... [more]
Q149L61.3e-3633.33DnaJ homolog subfamily B member 14 OS=Mus musculus OX=10090 GN=Dnajb14 PE=2 SV=1[more]
Q8TBM81.1e-3532.11DnaJ homolog subfamily B member 14 OS=Homo sapiens OX=9606 GN=DNAJB14 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S4E2R17.9e-18191.19chaperone protein dnaJ 49-like OS=Cucumis melo OX=3656 GN=LOC103497850 PE=4 SV=1[more]
A0A0A0KG462.8e-17890.06J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G306340 PE=4 SV=1[more]
A0A6J1H4U82.3e-17287.22chaperone protein dnaJ 49 OS=Cucurbita moschata OX=3662 GN=LOC111460072 PE=4 SV=... [more]
A0A6J1KXF32.5e-17186.65chaperone protein dnaJ 49 OS=Cucurbita maxima OX=3661 GN=LOC111499080 PE=4 SV=1[more]
A0A6J1CB721.6e-17086.78chaperone protein dnaJ 49 OS=Momordica charantia OX=3673 GN=LOC111009903 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT5G49060.12.9e-9551.98Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) [more]
AT3G57340.14.6e-6438.48Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) [more]
AT3G57340.24.6e-6438.48Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) [more]
AT5G05750.14.1e-4938.25DNAJ heat shock N-terminal domain-containing protein [more]
AT2G22360.18.2e-1347.14DNAJ heat shock family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001623DnaJ domainPRINTSPR00625JDOMAINcoord: 125..140
score: 51.05
coord: 107..125
score: 51.32
coord: 141..161
score: 31.96
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 104..161
e-value: 3.5E-24
score: 96.4
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 105..166
e-value: 5.5E-23
score: 80.9
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 105..169
score: 19.748743
IPR001623DnaJ domainCDDcd06257DnaJcoord: 105..158
e-value: 2.90733E-20
score: 81.0525
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 102..221
e-value: 3.3E-29
score: 103.7
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 101..212
IPR015399Domain of unknown function DUF1977, DnaJ-likePFAMPF09320DUF1977coord: 263..333
e-value: 1.3E-7
score: 31.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 53..73
NoneNo IPR availablePANTHERPTHR43908:SF5CHAPERONE PROTEIN DNAJ 49coord: 1..351
NoneNo IPR availablePANTHERPTHR43908AT29763P-RELATEDcoord: 1..351

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10010356.1HG10010356.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071218 cellular response to misfolded protein
biological_process GO:0051085 chaperone cofactor-dependent protein refolding
biological_process GO:0030433 ubiquitin-dependent ERAD pathway
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0030544 Hsp70 protein binding