HG10010285 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10010285 vs. NCBI nr
Match: KAG6593916.1 (hypothetical protein SDJN03_13392, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 568.5 bits (1464), Expect = 4.1e-158
Identity = 300/314 (95.54%), Postives = 303/314 (96.50%), Query Frame = 0

Query: 56  RVGNLANLGGLRARLSLKHSVSDHNDDEEECRPWRRRLSMAFTWRSALKITLLLLLLAAA 115
           R  NLANLGGLRARLSLKHSVSDHNDDEE CRPWRRRL MAFTWRSALKIT  LLLLAA 
Sbjct: 62  RPRNLANLGGLRARLSLKHSVSDHNDDEEVCRPWRRRLFMAFTWRSALKITAALLLLAAV 121

Query: 116 VTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSVAYIPLTVLAVPASILTLGGGYLFGLP 175
           VTACFTLPIEKILKDFLLWV +DLGAWGPLVLSVAYIPLTVLAVPASILTLGGGYLFGLP
Sbjct: 122 VTACFTLPIEKILKDFLLWVDQDLGAWGPLVLSVAYIPLTVLAVPASILTLGGGYLFGLP 181

Query: 176 VGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHSVAIAINRSGFKIVLLLRLV 235
           VGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHSVAIAINRSGFKIVLLLRLV
Sbjct: 182 VGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHSVAIAINRSGFKIVLLLRLV 241

Query: 236 PLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVYVGTTLKDLSDVTHGWGEFSKT 295
           PLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVYVGTTLKDLSDVTHGWGEFSKT
Sbjct: 242 PLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVYVGTTLKDLSDVTHGWGEFSKT 301

Query: 296 RWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDAVDYLGLSSQLPIVAES-PVDLNQ 355
           RWAFI+LGLVVSVVLAYCVTRVAKAALEKALAENGDAVDYLG+SSQLPIVAES PVDLNQ
Sbjct: 302 RWAFIMLGLVVSVVLAYCVTRVAKAALEKALAENGDAVDYLGISSQLPIVAESAPVDLNQ 361

Query: 356 PLIIKIDSPQDDRK 369
           PLIIKIDSP DD K
Sbjct: 362 PLIIKIDSPPDDCK 375

BLAST of HG10010285 vs. NCBI nr
Match: KAG6575014.1 (hypothetical protein SDJN03_25653, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 554.7 bits (1428), Expect = 6.1e-154
Identity = 288/309 (93.20%), Postives = 297/309 (96.12%), Query Frame = 0

Query: 59  NLANLGGLRARLSLKHSVSDHNDDEEECRPWRRRLSMAFTWRSALKITLLLLLLAAAVTA 118
           NLANLGGLRARLSLKHSVSDHND+EEECRPWRRRL MAFTWRSALKIT LL+L+AAAV A
Sbjct: 10  NLANLGGLRARLSLKHSVSDHNDEEEECRPWRRRLFMAFTWRSALKITFLLILVAAAVIA 69

Query: 119 CFTLPIEKILKDFLLWVHEDLGAWGPLVLSVAYIPLTVLAVPASILTLGGGYLFGLPVGF 178
           CFTLPIEKILKDFLLWV +DLGAWGPLVLS+AYIPLTVLAVPASILTLGGGYLFGLPVGF
Sbjct: 70  CFTLPIEKILKDFLLWVDQDLGAWGPLVLSLAYIPLTVLAVPASILTLGGGYLFGLPVGF 129

Query: 179 VADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHSVAIAINRSGFKIVLLLRLVPLL 238
           VADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFH VAIAINRSGFKIVLLLRLVPLL
Sbjct: 130 VADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHLVAIAINRSGFKIVLLLRLVPLL 189

Query: 239 PFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVYVGTTLKDLSDVTHGWGEFSKTRWA 298
           PFNMLNYLLSVTPVSLGEYMLASWLGMMPIT ALVYVGTTLKDLSDVTHGWGE SKTR A
Sbjct: 190 PFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVYVGTTLKDLSDVTHGWGELSKTRLA 249

Query: 299 FIILGLVVSVVLAYCVTRVAKAALEKALAENGDAVDYLGLSSQLPIVAESPVDLNQPLII 358
           FIILGLVVS +LAYC+TRVAKAALEKALAENGDAVDYLGLS +LP+ AESPVDLN PLII
Sbjct: 250 FIILGLVVSALLAYCITRVAKAALEKALAENGDAVDYLGLSPRLPVAAESPVDLNLPLII 309

Query: 359 KIDSPQDDR 368
           KIDSPQDDR
Sbjct: 310 KIDSPQDDR 318

BLAST of HG10010285 vs. NCBI nr
Match: KAG7013587.1 (hypothetical protein SDJN02_23754, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 538.5 bits (1386), Expect = 4.5e-149
Identity = 288/343 (83.97%), Postives = 298/343 (86.88%), Query Frame = 0

Query: 59  NLANLGGLRARLSLKHSVSDHNDDEEECRPWRRRLSMAFTWRSALKITLLLLLLAAAVTA 118
           NLANLGGLRARLSLKHSVSDHND+EEECRPWRRRL MAFTWRSALKIT LL+L+AAAV A
Sbjct: 9   NLANLGGLRARLSLKHSVSDHNDEEEECRPWRRRLFMAFTWRSALKITFLLILVAAAVIA 68

Query: 119 CFTLPIEK----------------------------------ILKDFLLWVHEDLGAWGP 178
           CFTLPIEK                                  ILKDFLLWV +DLGAWGP
Sbjct: 69  CFTLPIEKAGRFMTNYSFLIFNLMSYDLYRIDHIFENLTPLQILKDFLLWVDQDLGAWGP 128

Query: 179 LVLSVAYIPLTVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFV 238
           LVLS+AYIPLTVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFV
Sbjct: 129 LVLSLAYIPLTVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFV 188

Query: 239 VSKLKDYPQFHSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLG 298
           VSKLKDYPQFHSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLG
Sbjct: 189 VSKLKDYPQFHSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLG 248

Query: 299 MMPITFALVYVGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEK 358
           MMPIT ALVYVGTTLKDLSDVTHGWGE SKTR AFIILGLVVS +LAYC+TRVAKAALEK
Sbjct: 249 MMPITLALVYVGTTLKDLSDVTHGWGELSKTRLAFIILGLVVSALLAYCITRVAKAALEK 308

Query: 359 ALAENGDAVDYLGLSSQLPIVAESPVDLNQPLIIKIDSPQDDR 368
           ALAENGDAVDYLGLS +LP+ AE+PVDLN PLIIKIDSPQDDR
Sbjct: 309 ALAENGDAVDYLGLSPRLPVAAEAPVDLNLPLIIKIDSPQDDR 351

BLAST of HG10010285 vs. NCBI nr
Match: XP_008458555.1 (PREDICTED: TVP38/TMEM64 family membrane protein slr0305-like [Cucumis melo] >XP_008458556.1 PREDICTED: TVP38/TMEM64 family membrane protein slr0305-like [Cucumis melo])

HSP 1 Score: 509.6 bits (1311), Expect = 2.2e-140
Identity = 270/275 (98.18%), Postives = 271/275 (98.55%), Query Frame = 0

Query: 95  MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSVAYIPL 154
           MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLS+AYIPL
Sbjct: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60

Query: 155 TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 214
           TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 215 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVY 274
           HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPIT ALVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 180

Query: 275 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDAVD 334
           VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGD VD
Sbjct: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240

Query: 335 YLGLSSQLPIVAESPVDLNQPLIIKIDSPQ-DDRK 369
           YLGLSSQLPIV ESPVDLNQPLIIKIDSPQ DDRK
Sbjct: 241 YLGLSSQLPIVDESPVDLNQPLIIKIDSPQVDDRK 275

BLAST of HG10010285 vs. NCBI nr
Match: XP_004150898.1 (uncharacterized protein LOC101204495 [Cucumis sativus] >XP_011657201.1 uncharacterized protein LOC101204495 [Cucumis sativus] >KGN47260.1 hypothetical protein Csa_017041 [Cucumis sativus])

HSP 1 Score: 508.8 bits (1309), Expect = 3.8e-140
Identity = 267/272 (98.16%), Postives = 268/272 (98.53%), Query Frame = 0

Query: 95  MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSVAYIPL 154
           MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLS+AYIPL
Sbjct: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60

Query: 155 TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 214
           TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 215 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVY 274
           HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPIT  LVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVY 180

Query: 275 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDAVD 334
           VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGD VD
Sbjct: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240

Query: 335 YLGLSSQLPIVAESPVDLNQPLIIKIDSPQDD 367
           YLGLSSQLPIV ESPVDLNQPLIIKIDSPQDD
Sbjct: 241 YLGLSSQLPIVDESPVDLNQPLIIKIDSPQDD 272

BLAST of HG10010285 vs. ExPASy Swiss-Prot
Match: Q55909 (TVP38/TMEM64 family membrane protein slr0305 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr0305 PE=3 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.7e-29
Identity = 76/200 (38.00%), Postives = 122/200 (61.00%), Query Frame = 0

Query: 128 LKDFLLWVHEDLGAWGPLVLSVAYIPLTVLAVPASILTLGGGYLFGLPVGFVADSIGATV 187
           L + L W+ + LG W  +   + Y   TV+ +P SILTLG G +FG+ +G +   IGAT+
Sbjct: 5   LLNALQWI-DGLGTWAAIAFMLLYTVATVVFLPGSILTLGAGVVFGVILGSIYVFIGATL 64

Query: 188 GAGAAFLLGRTIGKSFVVSKLKDYPQFHSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLL 247
           GA AAFL+GR + + +V  K+    +F ++  A+ + G KIV+L RL P+ PFN+LNY  
Sbjct: 65  GATAAFLVGRYLARGWVAKKIAGNQKFKAIDEAVGKEGLKIVILTRLSPVFPFNLLNYAY 124

Query: 248 SVTPVSLGEYMLASWLGMMPITFALVYVGTTLKDLSDVTHGWGEFSKT-RWAFIILGLVV 307
            +T VSL +Y++ S LGM+P T   VY+G+    L+ +     + + T +W   I+G + 
Sbjct: 125 GITNVSLKDYVIGS-LGMIPGTIMYVYIGSLAGSLATLGTATNQANPTLQWTIRIVGFIA 184

Query: 308 SVVLAYCVTRVAKAALEKAL 327
           +V +   VT++A+ AL +A+
Sbjct: 185 TVAVTIYVTKIARKALNEAI 202

BLAST of HG10010285 vs. ExPASy Swiss-Prot
Match: Q6YI46 (Transmembrane protein 64 OS=Homo sapiens OX=9606 GN=TMEM64 PE=2 SV=2)

HSP 1 Score: 78.6 bits (192), Expect = 1.7e-13
Identity = 70/247 (28.34%), Postives = 123/247 (49.80%), Query Frame = 0

Query: 85  ECRPWRRRLSMAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGP 144
           E R WR     +  W  +L   +L+ +LAA   A   L + + L   LLWV       G 
Sbjct: 105 EVRNWRCCCLGSTCWCRSL---VLVCVLAALCFASLAL-VRRYLHHLLLWVESLDSLLGV 164

Query: 145 LVLSVAYIPLTVLAVPAS----ILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIG 204
           L+  V +I   V++ P      +L +  GYL+G  +G     +G  +G   A ++ + + 
Sbjct: 165 LLFVVGFI---VVSFPCGWGYIVLNVAAGYLYGFVLGMGLMMVGVLIGTFIAHVVCKRLL 224

Query: 205 KSFVVSKLKDYPQFHSV-AIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYML 264
            ++V ++++   +  +V  +    SG K+V L RL P +PF + N + S+T +SL  Y++
Sbjct: 225 TAWVAARIQSSEKLSAVIRVVEGGSGLKVVALARLTP-IPFGLQNAVFSITDLSLPNYLM 284

Query: 265 ASWLGMMPITFALVYVGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAK 324
           AS +G++P      Y+GTTL+ + DV     E S + +    L +++S+ L + V   A+
Sbjct: 285 ASSVGLLPTQLLNSYLGTTLRTMEDVI---AEQSVSGYFVFCLQIIISIGLMFYVVHRAQ 340

Query: 325 AALEKAL 327
             L  A+
Sbjct: 345 VELNAAI 340

BLAST of HG10010285 vs. ExPASy Swiss-Prot
Match: P76219 (TVP38/TMEM64 family membrane protein YdjX OS=Escherichia coli (strain K12) OX=83333 GN=ydjX PE=3 SV=2)

HSP 1 Score: 69.7 bits (169), Expect = 7.8e-11
Identity = 48/166 (28.92%), Postives = 83/166 (50.00%), Query Frame = 0

Query: 104 KITLLLLLLAAAVTACFTLPIEKILKDF--LLWVHEDLGAWGPLVLSVAYIPLTVLAVPA 163
           K     L+ A  + A     +  +L D   L  +    G +G  +  + +I  T+L +P 
Sbjct: 6   KFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSGFFGYSLYILLFIIATLLLLPG 65

Query: 164 SILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHSVAIAI 223
           SIL + GG +FG  +G +   I AT+ +  +FLL R +G+  ++  +     F ++   I
Sbjct: 66  SILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARWLGRDLLLKYVGHSNTFQAIEKGI 125

Query: 224 NRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMP 268
            R+G   ++L RL+PL P+N+ NY   +T ++   Y L S L  +P
Sbjct: 126 ARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWPYTLISALTTLP 171

BLAST of HG10010285 vs. ExPASy Swiss-Prot
Match: P9WFS2 (TVP38/TMEM64 family membrane protein MT1538 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT1538 PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 3.6e-08
Identity = 50/171 (29.24%), Postives = 83/171 (48.54%), Query Frame = 0

Query: 106 TLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSVAYIPLTVLAVPASILT 165
           T++ + +   V     +P    L+D   W  + LGAW PL   + +  +TV   P +  T
Sbjct: 35  TVVGITVLVVVALLVPVPTAVELRD---WA-KSLGAWFPLAFLLVHTVVTVPPFPRTAFT 94

Query: 166 LGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHSVAIAINRSG 225
           L  G LFG  VG     +G+T  A  A LL R  G  + ++ L      + +   +   G
Sbjct: 95  LAAGLLFGSVVGVFIAVVGSTASAVIAMLLVRATG--WQLNSLVRRRAINRLDERLRERG 154

Query: 226 FKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVYVG 277
           +  +L LRL+P++PF  +NY    + V +  +  A+  G++P T A+V +G
Sbjct: 155 WLAILSLRLIPVVPFAAINYAAGASGVRILSFAWATLAGLLPGTAAVVILG 199

BLAST of HG10010285 vs. ExPASy Swiss-Prot
Match: P9WFS3 (TVP38/TMEM64 family membrane protein Rv1491c OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv1491c PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 3.6e-08
Identity = 50/171 (29.24%), Postives = 83/171 (48.54%), Query Frame = 0

Query: 106 TLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSVAYIPLTVLAVPASILT 165
           T++ + +   V     +P    L+D   W  + LGAW PL   + +  +TV   P +  T
Sbjct: 35  TVVGITVLVVVALLVPVPTAVELRD---WA-KSLGAWFPLAFLLVHTVVTVPPFPRTAFT 94

Query: 166 LGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHSVAIAINRSG 225
           L  G LFG  VG     +G+T  A  A LL R  G  + ++ L      + +   +   G
Sbjct: 95  LAAGLLFGSVVGVFIAVVGSTASAVIAMLLVRATG--WQLNSLVRRRAINRLDERLRERG 154

Query: 226 FKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVYVG 277
           +  +L LRL+P++PF  +NY    + V +  +  A+  G++P T A+V +G
Sbjct: 155 WLAILSLRLIPVVPFAAINYAAGASGVRILSFAWATLAGLLPGTAAVVILG 199

BLAST of HG10010285 vs. ExPASy TrEMBL
Match: A0A1S3C8Q0 (TVP38/TMEM64 family membrane protein slr0305-like OS=Cucumis melo OX=3656 GN=LOC103497924 PE=4 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 1.1e-140
Identity = 270/275 (98.18%), Postives = 271/275 (98.55%), Query Frame = 0

Query: 95  MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSVAYIPL 154
           MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLS+AYIPL
Sbjct: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60

Query: 155 TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 214
           TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 215 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVY 274
           HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPIT ALVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 180

Query: 275 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDAVD 334
           VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGD VD
Sbjct: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240

Query: 335 YLGLSSQLPIVAESPVDLNQPLIIKIDSPQ-DDRK 369
           YLGLSSQLPIV ESPVDLNQPLIIKIDSPQ DDRK
Sbjct: 241 YLGLSSQLPIVDESPVDLNQPLIIKIDSPQVDDRK 275

BLAST of HG10010285 vs. ExPASy TrEMBL
Match: A0A0A0KC10 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G237630 PE=4 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 1.9e-140
Identity = 267/272 (98.16%), Postives = 268/272 (98.53%), Query Frame = 0

Query: 95  MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSVAYIPL 154
           MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLS+AYIPL
Sbjct: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60

Query: 155 TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 214
           TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 215 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVY 274
           HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPIT  LVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVY 180

Query: 275 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDAVD 334
           VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGD VD
Sbjct: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240

Query: 335 YLGLSSQLPIVAESPVDLNQPLIIKIDSPQDD 367
           YLGLSSQLPIV ESPVDLNQPLIIKIDSPQDD
Sbjct: 241 YLGLSSQLPIVDESPVDLNQPLIIKIDSPQDD 272

BLAST of HG10010285 vs. ExPASy TrEMBL
Match: A0A6J1KD88 (uncharacterized protein LOC111494568 OS=Cucurbita maxima OX=3661 GN=LOC111494568 PE=4 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 1.1e-137
Identity = 265/275 (96.36%), Postives = 268/275 (97.45%), Query Frame = 0

Query: 95  MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSVAYIPL 154
           MAFTWRSALKIT  LLLLAA VTACFTLPIEKILKDFLLWV +DLGAWGPLVLSVAYIPL
Sbjct: 1   MAFTWRSALKITAALLLLAAVVTACFTLPIEKILKDFLLWVDQDLGAWGPLVLSVAYIPL 60

Query: 155 TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 214
           TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 215 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVY 274
           HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVY 180

Query: 275 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDAVD 334
           VGTTLKDLSDVTHGWGEFSKTRWAFI+LGLVVSVVLAYCVTRVAKAALEKALAENGDAVD
Sbjct: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIMLGLVVSVVLAYCVTRVAKAALEKALAENGDAVD 240

Query: 335 YLGLSSQLPIVAES-PVDLNQPLIIKIDSPQDDRK 369
           YLG+SSQLPIVAES PVDLNQPLIIKIDSP DD K
Sbjct: 241 YLGISSQLPIVAESAPVDLNQPLIIKIDSPPDDSK 275

BLAST of HG10010285 vs. ExPASy TrEMBL
Match: A0A6J1EQJ6 (uncharacterized protein LOC111436796 OS=Cucurbita moschata OX=3662 GN=LOC111436796 PE=4 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 1.9e-137
Identity = 265/275 (96.36%), Postives = 268/275 (97.45%), Query Frame = 0

Query: 95  MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSVAYIPL 154
           MAFTWRSALKIT  LLLLAA VTACFTLPIEKILKDFLLWV +DLGAWGPLVLSVAYIPL
Sbjct: 1   MAFTWRSALKITAALLLLAAVVTACFTLPIEKILKDFLLWVDQDLGAWGPLVLSVAYIPL 60

Query: 155 TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 214
           TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 215 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVY 274
           HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVY 180

Query: 275 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDAVD 334
           VGTTLKDLSDVTHGWGEFSKTRWAFI+LGLVVSVVLAYCVTRVAKAALEKALAENGDAVD
Sbjct: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIMLGLVVSVVLAYCVTRVAKAALEKALAENGDAVD 240

Query: 335 YLGLSSQLPIVAES-PVDLNQPLIIKIDSPQDDRK 369
           YLG+SSQLPIVAES PVDLNQPLIIKIDSP DD K
Sbjct: 241 YLGISSQLPIVAESAPVDLNQPLIIKIDSPPDDCK 275

BLAST of HG10010285 vs. ExPASy TrEMBL
Match: A0A498IBM5 (Uncharacterized protein OS=Malus domestica OX=3750 GN=DVH24_041506 PE=4 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 9.5e-137
Identity = 263/338 (77.81%), Postives = 294/338 (86.98%), Query Frame = 0

Query: 31  GRCGPNQQAQKAEWKEKETGNLERQRVG---NLANLGGLRARLSLKHSVSDHNDDEEECR 90
           GR   N + QK +W  ++    +    G   NLANLGGLR RLSLK  +  +NDD+EECR
Sbjct: 32  GREDKNARKQK-QWVPRKKRGRKLTSFGAESNLANLGGLRPRLSLKQPI--YNDDDEECR 91

Query: 91  PWRRRLSMAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVL 150
           PWRRR SMAFTW SAL+I LLLLLLAA  TACFTLP+EKILKDFLLWV +DLG WGPLVL
Sbjct: 92  PWRRRFSMAFTWGSALRIALLLLLLAAVATACFTLPVEKILKDFLLWVEQDLGPWGPLVL 151

Query: 151 SVAYIPLTVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSK 210
           +VAYIPLTVLAVPAS+LTLGGGYLFGLPVGFVADSIGAT+GAGAAFLLGRTIG+SFVVS+
Sbjct: 152 AVAYIPLTVLAVPASVLTLGGGYLFGLPVGFVADSIGATLGAGAAFLLGRTIGRSFVVSR 211

Query: 211 LKDYPQFHSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMP 270
           LKDYPQF +VAIAI RSGFKIVLLLRLVPLLPFNMLNYLLSVTPV +G+YMLASW+GMMP
Sbjct: 212 LKDYPQFRAVAIAIRRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVPIGQYMLASWIGMMP 271

Query: 271 ITFALVYVGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALA 330
           ITFALVYVGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSV+L  CVTRVAKAALEKALA
Sbjct: 272 ITFALVYVGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVILMICVTRVAKAALEKALA 331

Query: 331 ENGDAVDYLGLSSQLPIVAESPVDLNQPLIIKIDSPQD 366
           EN D +D + +  +LP+V+E+PVDL+QPL+IKID+  D
Sbjct: 332 ENED-IDNIDIIPELPVVSETPVDLDQPLLIKIDASDD 365

BLAST of HG10010285 vs. TAIR 10
Match: AT5G19070.1 (SNARE associated Golgi protein family )

HSP 1 Score: 382.1 bits (980), Expect = 5.0e-106
Identity = 197/274 (71.90%), Postives = 233/274 (85.04%), Query Frame = 0

Query: 95  MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSVAYIPL 154
           MAFTW SAL+I++LL+L+AA V AC+ LP+EK+LKDFLLWV +DLG WGP  L+VAYIPL
Sbjct: 1   MAFTWGSALRISVLLILVAAIVLACYFLPVEKLLKDFLLWVEQDLGPWGPFALAVAYIPL 60

Query: 155 TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 214
           TVLAVPAS+LTLGGGYLFGLP+GFVADS+GAT+G+GAAFLLGRTIGK FVV+KLKDYPQF
Sbjct: 61  TVLAVPASVLTLGGGYLFGLPIGFVADSVGATLGSGAAFLLGRTIGKPFVVAKLKDYPQF 120

Query: 215 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVY 274
            SVA+AI +SGFKI LLLRL PLLPF+MLNYLLSVTP+ LG Y+L+SWLGMMPIT ALVY
Sbjct: 121 QSVALAIEKSGFKICLLLRLAPLLPFSMLNYLLSVTPIRLGPYLLSSWLGMMPITLALVY 180

Query: 275 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAEN-GDAV 334
           VGTTLKDLSDVTH W EFS  RWAF+I  LV+SV+L  CVT+VAK AL KALAE+ GD  
Sbjct: 181 VGTTLKDLSDVTHKWSEFSPGRWAFLISSLVISVILMVCVTKVAKDALRKALAEHGGDMN 240

Query: 335 DYLGLSSQLPIVAESPVDLNQPLIIKIDS--PQD 366
           + +    +L +  ++  DLN+PL+IKID+  PQD
Sbjct: 241 EAVAALPELTVTDDASTDLNEPLLIKIDAQQPQD 274

BLAST of HG10010285 vs. TAIR 10
Match: AT1G03260.1 (SNARE associated Golgi protein family )

HSP 1 Score: 349.4 bits (895), Expect = 3.6e-96
Identity = 184/270 (68.15%), Postives = 227/270 (84.07%), Query Frame = 0

Query: 94  SMAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSVAYIP 153
           +M+FT  S  +I + LLLL A V+A   LP+E+ LKDFLLW+ EDLG +GPL L++AYIP
Sbjct: 3   AMSFT-PSTFRIAISLLLLVAIVSAVIFLPVEQKLKDFLLWIKEDLGPFGPLALALAYIP 62

Query: 154 LTVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQ 213
           LT++AVPAS+LTLGGGYLFGLPVGFVADS+GAT+GA AAFLLGRTIGKS+V SK+K YP+
Sbjct: 63  LTIVAVPASVLTLGGGYLFGLPVGFVADSLGATLGATAAFLLGRTIGKSYVTSKIKHYPK 122

Query: 214 FHSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALV 273
           F +V++AI +SGFKIVLLLR+VP+LPFNMLNYLLSVTPV LGEYMLA+WLGMMPITFALV
Sbjct: 123 FQAVSVAIQKSGFKIVLLLRVVPILPFNMLNYLLSVTPVRLGEYMLATWLGMMPITFALV 182

Query: 274 YVGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDAV 333
           YVGTTLKDLSD+THGW E S  RW  +++G+ ++V+L  C+TRVAK++L+KALAENG  +
Sbjct: 183 YVGTTLKDLSDITHGWHEVSVFRWVIMMVGVALAVILIICITRVAKSSLDKALAENGTEL 242

Query: 334 D--YLGLSSQLPIVAESPVDLNQPLIIKID 362
           D      +S LPI AE P DL +PL+I+ID
Sbjct: 243 DGKKNDDASVLPI-AEPPPDLQEPLVIRID 270

BLAST of HG10010285 vs. TAIR 10
Match: AT1G22850.1 (SNARE associated Golgi protein family )

HSP 1 Score: 95.5 bits (236), Expect = 9.4e-20
Identity = 78/234 (33.33%), Postives = 125/234 (53.42%), Query Frame = 0

Query: 101 SALKITLL--LLLLA-----AAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSVAYIP 160
           +A+K T+L  +LL+      A V   +   I   L  F  ++ E  G  G  +    Y  
Sbjct: 104 AAIKGTILAGVLLIGTVGGFAGVGYVYRDQINTFLTQFSTYI-EGYGTAGYALFIAVYAG 163

Query: 161 LTVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQ 220
           L +LA+PA  LT+  G LFG  +G +  SI  T+ A  AFL+ R   +  ++  ++D  +
Sbjct: 164 LEILAIPALPLTMSAGLLFGPLIGTIIVSISGTMAASVAFLIARYFARERILKLVEDNKK 223

Query: 221 FHSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALV 280
           F ++  AI  +GF++V LLRL PLLPF++ NYL  +T V    Y+L SWLGM+P ++A V
Sbjct: 224 FLAIDKAIGENGFRVVTLLRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGSWAYV 283

Query: 281 YVG----TTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALE 324
             G      +++ S+V    G     +   + +GL+V+ +    VT +AK A++
Sbjct: 284 SAGAFGRAIIQEESNVGLPGG---NGQLLTLGVGLLVTALAGTYVTSLAKDAIK 333

BLAST of HG10010285 vs. TAIR 10
Match: AT1G12450.1 (SNARE associated Golgi protein family )

HSP 1 Score: 58.5 bits (140), Expect = 1.3e-08
Identity = 64/202 (31.68%), Postives = 93/202 (46.04%), Query Frame = 0

Query: 124 IEKILKDFLLWVHE--DLGAWGPLVL-SVAYIPLTVLAVPASILTLGGGYLFGLPVGFVA 183
           IEK L  F+ WV     +   G L+  SVA  P  +L  P+S      G  FG   GF+ 
Sbjct: 85  IEKELIPFINWVRNTFSIPVLGLLLFASVALFPSILL--PSSPSMWMAGLTFGYGKGFLL 144

Query: 184 DSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHSVAIAINRS----GFKIVLLLRLVP 243
               A++G    FL+G       +   LK YP+  ++  A         F+ V L+R+ P
Sbjct: 145 ILSAASIGVTLPFLIGHLFLHK-MQEWLKKYPKKAAILRAAGEGTWFHQFQAVTLIRVSP 204

Query: 244 LLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVYVGTTLKDLSDVTHGWGEFSKTR 303
             P+ + NY    T V  G Y+L S +GM+P  F  +Y G  L+ L+         S TR
Sbjct: 205 -FPYIIYNYCALATGVHYGPYILGSLVGMVPEIFVSIYTGIMLRTLAVA-------SDTR 264

Query: 304 WAFIILGLVVSVVLAYCVTRVA 319
               ++ +VV+ VL +CVT  A
Sbjct: 265 HTLSVVEIVVN-VLGFCVTASA 274

BLAST of HG10010285 vs. TAIR 10
Match: AT4G09580.1 (SNARE associated Golgi protein family )

HSP 1 Score: 55.1 bits (131), Expect = 1.4e-07
Identity = 57/223 (25.56%), Postives = 94/223 (42.15%), Query Frame = 0

Query: 80  NDDEEECRPWRRRLSMAFTWRSALKITLLLLLLAAAVTACFTLP--------IEKILKDF 139
           NDD    +  +        W  A+  T+ L+          T+P        + + + D 
Sbjct: 34  NDDSPTGKRTKSERFPLSRWEFAVFFTVFLVFTTGLFCIYLTMPAAEYGKLKVPRTISDL 93

Query: 140 LLWVHEDLGAWGP-------LVLSVAYIPLTVLAVPASI-LTLGGGYLFGLPVGFVADSI 199
            L + E+LG++         L     YI +    +P +I ++L  G LFG+  GFV   +
Sbjct: 94  RL-LKENLGSYASEYQARFILGYCSTYIFMQTFMIPGTIFMSLLAGALFGVVRGFVLVVL 153

Query: 200 GATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHSVAIAINRSG-FKIVLLLRLVPLLPFNM 259
            AT GA + F L + +G+  V     +  +F    IA  R      +L LR+ P LP   
Sbjct: 154 NATAGACSCFFLSKLVGRPLVNWLWPEKLRFFQAEIAKRRDRLLNYMLFLRITPTLPNLF 213

Query: 260 LNYLLSVTPVSLGEYMLASWLGMMPITFALVYVGTTLKDLSDV 286
           +N    +  +    + LA+ +G+MP ++  V  G  L DL  V
Sbjct: 214 INLSSPIVDIPFHVFFLATLVGLMPASYITVRAGLALGDLRSV 255