HG10010216 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10010216
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionNAD(P)-linked oxidoreductase superfamily protein
LocationChr06: 19968085 .. 19970185 (+)
RNA-Seq ExpressionHG10010216
SyntenyHG10010216
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGGAAAGGTCTGTCAAAGGCAATCAGTGGGGAAAAGCAAAAAGATCATCATTCAGTTTAAAGTTTAAGAATAGAGAACCCAATAGCCTCGAAACTCCTCTTTTCTTTTTCACAGGCAATTGCAACTTCTGCAAACTAAGCTTTTTGATCATAGTGAAATGGAGGAAAAGCCTCAAAATCTGAGCCAAATCCCGAGAGTGAAGCTGGGTAGCCAAGGACTGGAGGTAAGCTTAATCACATTTGAGAAATAGGAACTAGGAAAAACAAACAATAAAAGTTGGGAGAATCCCAATATTTTGGTTTGATTGGGATTTACCAAGCAGAAGTTCGATCTTGATTTGATGGGATTTGAATATTATTCTTTGTGAAATGGTTGCAGGTTTCAAGGTTGGGTTTCGGATGTGCAGGACTATCGGGACTTTACAATGCTCCCCTAACTCATGAAGGTGGATGTCGCATGATTAAACAAGTTTTCACTAGAGGCATAACCTTTTTCGATACTTCCGATCTTTATGGAGCCGATCATGACAATGAAATCATGGTCGGAAAGGTGTGTTTTCTAATAACACAAACTTTGAAAAGTTGTGGAGTTTGAAACCTTACCAATTAGCTGAAGATGTTCATATGCCAGGCTTTAAAACAGCTTCCTCGAGAGAAGATCCAGTTAGCTACAAAATTTGGCATTGTGCCATTAGGAAGCTTTCAATTTGCAGTAAATGGGACACCTGAATATGTAAGGAAGTGCTGTGAAGCAAGTCTCGAGCGGCTTCAAGTTGATCACATTGACCTCTATTATCAGCATCGCATCGACCTATCTGTGCCAATTGAGGAAACTGTGAGCCCTCTCTTCCATGGACGACTATATTGAGCTTTCTTTTTCCCACTGTCTGAAAATAACAATCAACTTTTTGAAATTTTGATGAATGACTATGAAGATGGGAGAGTTGAAGAAGCTGGTGGAGGAGGGGAAGATAAAGTACATCGGTCTGTCTGAAGCGAGTGCAGACATGATCCGGAGAGCCCATGCAGTTCATCCAATCACTGCCTTACAGATGGAGTATTCCCTGTGGTGCCGTGAAACTGAAGATGAGATAATCCCACTTTGCAGGTGAGACAAGGCAACTAGATAATACAAAGTTATATCTTGACAGATTTAGTATATGAAGGGATGGTGTTTTGTAGAGAGCTTGGAATTGGGATAGTGGCTTACAGCCCTTTGGGTCGAGGCTTCTTTGGCGGAAAGGCGGTTATAGAGAGCTTGCCCAGTGAGAGCTTGCTGGTAAGTTTGTAAGAGGAAAATTCAGAGCAAGTTAATCTGCTTATCATAACTCGTGGATATGTTGTGTTGACAGGTTAAACATCCAAGGTTCAGCAAGGATAGTTTAAAAGTTAATGAAACTATCTATGAACGGCTTGTTGATCTGGCTGCCAAGCATCGCTGCACGACTGCTCAGTTGGCTTTAGCATGGCTGCTTCATCAGGGTATTGATATAGTTCCCGTCCCAGGTACGGTAAATATCTTCCTCTTCCTGACCAAAATGGAGTTACCAAAATGGAGTTGTAAGAATGTTAATTGTTGAAGAATGCCAATAACAGATAGCAAAGAGAGAGAAACGTTCACCATGACATCTTGAATGATAACATTTGAAATAAAAATAAGATTAAAGTTAATCTAATGGTGGCTAGTAGGCATAGAATATCAAAATAGGAGATCTAGAATTCAAATCTCAGCAATGTCATTCTCTCTCCATGTCACACTTACAGTCTATATATCAACTGATCTAGTAAGTAAAATTAATATAATGTAGAATGTTGAGAAAAGATTGAATTGTGTCCGATAGTTTGATTCTGTAAATGAAGGGTAAAGTTTGTTCTGTTGACAGTTCAAACTCGTTGTTTCATTTCCCATTCTGCAACAGGGACAACCAAAGTTGGAAACCTTGACAGCAACATTGGGTCACTAAGTGTGAAGCTAACAGAGAAGGATTTGAAAGAAATTAGTGATGCTGTGCCAATTGATGAAGTTAGAGGTGAGCGAGAATATGATGCATTCTCCAGCTATATGTGGAACTATGCAGGTTCCTCATCAAAGGGCTGA

mRNA sequence

ATGTTGGAAAGGCAATTGCAACTTCTGCAAACTAAGCTTTTTGATCATAGTGAAATGGAGGAAAAGCCTCAAAATCTGAGCCAAATCCCGAGAGTGAAGCTGGGTAGCCAAGGACTGGAGGTTTCAAGGTTGGGTTTCGGATGTGCAGGACTATCGGGACTTTACAATGCTCCCCTAACTCATGAAGGTGGATGTCGCATGATTAAACAAGTTTTCACTAGAGGCATAACCTTTTTCGATACTTCCGATCTTTATGGAGCCGATCATGACAATGAAATCATGGTCGGAAAGGCTTTAAAACAGCTTCCTCGAGAGAAGATCCAGTTAGCTACAAAATTTGGCATTGTGCCATTAGGAAGCTTTCAATTTGCAGTAAATGGGACACCTGAATATGTAAGGAAGTGCTGTGAAGCAAGTCTCGAGCGGCTTCAAGTTGATCACATTGACCTCTATTATCAGCATCGCATCGACCTATCTGTGCCAATTGAGGAAACTATGGGAGAGTTGAAGAAGCTGGTGGAGGAGGGGAAGATAAAGTACATCGGTCTGTCTGAAGCGAGTGCAGACATGATCCGGAGAGCCCATGCAGTTCATCCAATCACTGCCTTACAGATGGAGTATTCCCTGTGGTGCCGTGAAACTGAAGATGAGATAATCCCACTTTGCAGAGAGCTTGGAATTGGGATAGTGGCTTACAGCCCTTTGGGTCGAGGCTTCTTTGGCGGAAAGGCGGTTATAGAGAGCTTGCCCAGTGAGAGCTTGCTGGTTAAACATCCAAGGTTCAGCAAGGATAGTTTAAAAGTTAATGAAACTATCTATGAACGGCTTGTTGATCTGGCTGCCAAGCATCGCTGCACGACTGCTCAGTTGGCTTTAGCATGGCTGCTTCATCAGGGTATTGATATAGTTCCCGTCCCAGGGACAACCAAAGTTGGAAACCTTGACAGCAACATTGGGTCACTAAGTGTGAAGCTAACAGAGAAGGATTTGAAAGAAATTAGTGATGCTGTGCCAATTGATGAAGTTAGAGGTGAGCGAGAATATGATGCATTCTCCAGCTATATGTGGAACTATGCAGGTTCCTCATCAAAGGGCTGA

Coding sequence (CDS)

ATGTTGGAAAGGCAATTGCAACTTCTGCAAACTAAGCTTTTTGATCATAGTGAAATGGAGGAAAAGCCTCAAAATCTGAGCCAAATCCCGAGAGTGAAGCTGGGTAGCCAAGGACTGGAGGTTTCAAGGTTGGGTTTCGGATGTGCAGGACTATCGGGACTTTACAATGCTCCCCTAACTCATGAAGGTGGATGTCGCATGATTAAACAAGTTTTCACTAGAGGCATAACCTTTTTCGATACTTCCGATCTTTATGGAGCCGATCATGACAATGAAATCATGGTCGGAAAGGCTTTAAAACAGCTTCCTCGAGAGAAGATCCAGTTAGCTACAAAATTTGGCATTGTGCCATTAGGAAGCTTTCAATTTGCAGTAAATGGGACACCTGAATATGTAAGGAAGTGCTGTGAAGCAAGTCTCGAGCGGCTTCAAGTTGATCACATTGACCTCTATTATCAGCATCGCATCGACCTATCTGTGCCAATTGAGGAAACTATGGGAGAGTTGAAGAAGCTGGTGGAGGAGGGGAAGATAAAGTACATCGGTCTGTCTGAAGCGAGTGCAGACATGATCCGGAGAGCCCATGCAGTTCATCCAATCACTGCCTTACAGATGGAGTATTCCCTGTGGTGCCGTGAAACTGAAGATGAGATAATCCCACTTTGCAGAGAGCTTGGAATTGGGATAGTGGCTTACAGCCCTTTGGGTCGAGGCTTCTTTGGCGGAAAGGCGGTTATAGAGAGCTTGCCCAGTGAGAGCTTGCTGGTTAAACATCCAAGGTTCAGCAAGGATAGTTTAAAAGTTAATGAAACTATCTATGAACGGCTTGTTGATCTGGCTGCCAAGCATCGCTGCACGACTGCTCAGTTGGCTTTAGCATGGCTGCTTCATCAGGGTATTGATATAGTTCCCGTCCCAGGGACAACCAAAGTTGGAAACCTTGACAGCAACATTGGGTCACTAAGTGTGAAGCTAACAGAGAAGGATTTGAAAGAAATTAGTGATGCTGTGCCAATTGATGAAGTTAGAGGTGAGCGAGAATATGATGCATTCTCCAGCTATATGTGGAACTATGCAGGTTCCTCATCAAAGGGCTGA

Protein sequence

MLERQLQLLQTKLFDHSEMEEKPQNLSQIPRVKLGSQGLEVSRLGFGCAGLSGLYNAPLTHEGGCRMIKQVFTRGITFFDTSDLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLGSFQFAVNGTPEYVRKCCEASLERLQVDHIDLYYQHRIDLSVPIEETMGELKKLVEEGKIKYIGLSEASADMIRRAHAVHPITALQMEYSLWCRETEDEIIPLCRELGIGIVAYSPLGRGFFGGKAVIESLPSESLLVKHPRFSKDSLKVNETIYERLVDLAAKHRCTTAQLALAWLLHQGIDIVPVPGTTKVGNLDSNIGSLSVKLTEKDLKEISDAVPIDEVRGEREYDAFSSYMWNYAGSSSKG
Homology
BLAST of HG10010216 vs. NCBI nr
Match: XP_038906695.1 (LOW QUALITY PROTEIN: perakine reductase-like [Benincasa hispida])

HSP 1 Score: 671.8 bits (1732), Expect = 3.4e-189
Identity = 327/347 (94.24%), Postives = 339/347 (97.69%), Query Frame = 0

Query: 19  MEEKPQNLSQIPRVKLGSQGLEVSRLGFGCAGLSGLYNAPLTHEGGCRMIKQVFTRGITF 78
           MEEKPQN+SQIPRVKLGSQGLEVSRLGFGCAGLSGLYNAPLTHE GCRMIKQVFTRGITF
Sbjct: 1   MEEKPQNMSQIPRVKLGSQGLEVSRLGFGCAGLSGLYNAPLTHEDGCRMIKQVFTRGITF 60

Query: 79  FDTSDLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLGSFQFAVNGTPEYVRKCCEA 138
           FDTSDLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLGSFQFAVNGTPEYVRKCCEA
Sbjct: 61  FDTSDLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLGSFQFAVNGTPEYVRKCCEA 120

Query: 139 SLERLQVDHIDLYYQHRIDLSVPIEETMGELKKLVEEGKIKYIGLSEASADMIRRAHAVH 198
           SL+RLQVDHIDLYYQHR+DLSVPIEETMGELKKLVEEGKIKYIGLSEAS DMIRRAHAVH
Sbjct: 121 SLKRLQVDHIDLYYQHRVDLSVPIEETMGELKKLVEEGKIKYIGLSEASVDMIRRAHAVH 180

Query: 199 PITALQMEYSLWCRETEDEIIPLCRELGIGIVAYSPLGRGFFGGKAVIESLPSESLLVKH 258
           PITALQMEYSLW RETEDEIIPLCRELGIG+VAYSPLGRGFFGGKAV+E LPSESLLVKH
Sbjct: 181 PITALQMEYSLWSRETEDEIIPLCRELGIGVVAYSPLGRGFFGGKAVVECLPSESLLVKH 240

Query: 259 PRFSKDSLKVNETIYERLVDLAAKHRCTTAQLALAWLLHQGIDIVPVPGTTKVGNLDSNI 318
           PRFSK++L+VNET+Y+RLVDLAAKH CTTAQLALAWLLHQGIDIVP+PGTTKVGNLDSNI
Sbjct: 241 PRFSKENLEVNETMYKRLVDLAAKHSCTTAQLALAWLLHQGIDIVPIPGTTKVGNLDSNI 300

Query: 319 GSLSVKLTEKDLKEISDAVPIDEVRGEREYDAFSSYMWNYAGSSSKG 366
           GSL VKLTE+DLKEISDAVPIDEVRGEREYDAFS YMWNYA SSSKG
Sbjct: 301 GSLGVKLTEEDLKEISDAVPIDEVRGEREYDAFSRYMWNYADSSSKG 347

BLAST of HG10010216 vs. NCBI nr
Match: XP_022138494.1 (perakine reductase-like isoform X5 [Momordica charantia])

HSP 1 Score: 617.8 bits (1592), Expect = 5.8e-173
Identity = 298/342 (87.13%), Postives = 319/342 (93.27%), Query Frame = 0

Query: 23  PQNLSQIPRVKLGSQGLEVSRLGFGCAGLSGLYNAPLTHEGGCRMIKQVFTRGITFFDTS 82
           PQNL QIPRVKLGSQGLEVSRLGFGCAGLSG+YNAPLTH+ GC MIKQVFTRGITFFDTS
Sbjct: 2   PQNLIQIPRVKLGSQGLEVSRLGFGCAGLSGIYNAPLTHKDGCGMIKQVFTRGITFFDTS 61

Query: 83  DLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLGSFQFAVNGTPEYVRKCCEASLER 142
           DLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLG F+F+VNGTPEYVRK CEASLER
Sbjct: 62  DLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLGDFRFSVNGTPEYVRKSCEASLER 121

Query: 143 LQVDHIDLYYQHRIDLSVPIEETMGELKKLVEEGKIKYIGLSEASADMIRRAHAVHPITA 202
           LQVDHIDLYYQHR+D SVPIEETMGELKKLVEEGKIKYIGLSEASAD IRRAHAVHPITA
Sbjct: 122 LQVDHIDLYYQHRVDPSVPIEETMGELKKLVEEGKIKYIGLSEASADTIRRAHAVHPITA 181

Query: 203 LQMEYSLWCRETEDEIIPLCRELGIGIVAYSPLGRGFFGGKAVIESLPSESLLVKHPRFS 262
           LQMEYSLW R+ EDEIIPLCRELGIG+VAYSPLGRGFFGGKAV ESLPSESLLVKHPRF+
Sbjct: 182 LQMEYSLWSRDIEDEIIPLCRELGIGMVAYSPLGRGFFGGKAVAESLPSESLLVKHPRFA 241

Query: 263 KDSLKVNETIYERLVDLAAKHRCTTAQLALAWLLHQGIDIVPVPGTTKVGNLDSNIGSLS 322
           K+SL+ NE IY+RL+D+AAKHRC T QLALAWLLHQGIDIVP+PGTTKVGNLDSNI SL 
Sbjct: 242 KESLEENEAIYKRLLDMAAKHRCATVQLALAWLLHQGIDIVPIPGTTKVGNLDSNIRSLG 301

Query: 323 VKLTEKDLKEISDAVPIDEVRGEREYDAFSSYMWNYAGSSSK 365
           V+LT +DLKEI DAVP+DEVRG+REYD FS YMWN+A   ++
Sbjct: 302 VELTPEDLKEIGDAVPVDEVRGQREYDVFSKYMWNFADKGAE 343

BLAST of HG10010216 vs. NCBI nr
Match: XP_022138493.1 (perakine reductase-like isoform X4 [Momordica charantia])

HSP 1 Score: 609.0 bits (1569), Expect = 2.7e-170
Identity = 298/354 (84.18%), Postives = 319/354 (90.11%), Query Frame = 0

Query: 23  PQNLSQIPRVKLGSQGLEVSRLGFGCAGLSGLYNAPLTHEGGCRMIKQVFTRGITFFDTS 82
           PQNL QIPRVKLGSQGLEVSRLGFGCAGLSG+YNAPLTH+ GC MIKQVFTRGITFFDTS
Sbjct: 2   PQNLIQIPRVKLGSQGLEVSRLGFGCAGLSGIYNAPLTHKDGCGMIKQVFTRGITFFDTS 61

Query: 83  DLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLGSFQFAVNGTPEYVRKCCEASLER 142
           DLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLG F+F+VNGTPEYVRK CEASLER
Sbjct: 62  DLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLGDFRFSVNGTPEYVRKSCEASLER 121

Query: 143 LQVDHIDLYYQHRIDLSVPIEETMGELKKLVEEGKIKYIGLSEASADMIRRAHAVHPITA 202
           LQVDHIDLYYQHR+D SVPIEETMGELKKLVEEGKIKYIGLSEASAD IRRAHAVHPITA
Sbjct: 122 LQVDHIDLYYQHRVDPSVPIEETMGELKKLVEEGKIKYIGLSEASADTIRRAHAVHPITA 181

Query: 203 LQMEYSLWCRETEDEIIPLCRELGIGIVAYSPLGRGFFGGKAVIESLPSESLLVKHPRFS 262
           LQMEYSLW R+ EDEIIPLCRELGIG+VAYSPLGRGFFGGKAV ESLPSESLLVKHPRF+
Sbjct: 182 LQMEYSLWSRDIEDEIIPLCRELGIGMVAYSPLGRGFFGGKAVAESLPSESLLVKHPRFA 241

Query: 263 KDSLKVNETIYERLVDLAAKHRCTTAQLALAWLLHQGIDIVPVP------------GTTK 322
           K+SL+ NE IY+RL+D+AAKHRC T QLALAWLLHQGIDIVP+P            GTTK
Sbjct: 242 KESLEENEAIYKRLLDMAAKHRCATVQLALAWLLHQGIDIVPIPVPTLCFVFHSGKGTTK 301

Query: 323 VGNLDSNIGSLSVKLTEKDLKEISDAVPIDEVRGEREYDAFSSYMWNYAGSSSK 365
           VGNLDSNI SL V+LT +DLKEI DAVP+DEVRG+REYD FS YMWN+A   ++
Sbjct: 302 VGNLDSNIRSLGVELTPEDLKEIGDAVPVDEVRGQREYDVFSKYMWNFADKGAE 355

BLAST of HG10010216 vs. NCBI nr
Match: XP_022138492.1 (perakine reductase-like isoform X3 [Momordica charantia])

HSP 1 Score: 608.2 bits (1567), Expect = 4.6e-170
Identity = 298/356 (83.71%), Postives = 319/356 (89.61%), Query Frame = 0

Query: 23  PQNLSQIPRVKLGSQGLEVSRLGFGCAGLSGLYNAPLTHEGGCRMIKQVFTRGITFFDTS 82
           PQNL QIPRVKLGSQGLEVSRLGFGCAGLSG+YNAPLTH+ GC MIKQVFTRGITFFDTS
Sbjct: 2   PQNLIQIPRVKLGSQGLEVSRLGFGCAGLSGIYNAPLTHKDGCGMIKQVFTRGITFFDTS 61

Query: 83  DLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLGSFQFAVNGTPEYVRKCCEASLER 142
           DLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLG F+F+VNGTPEYVRK CEASLER
Sbjct: 62  DLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLGDFRFSVNGTPEYVRKSCEASLER 121

Query: 143 LQVDHIDLYYQHRIDLSVPIEET--------------MGELKKLVEEGKIKYIGLSEASA 202
           LQVDHIDLYYQHR+D SVPIEET              MGELKKLVEEGKIKYIGLSEASA
Sbjct: 122 LQVDHIDLYYQHRVDPSVPIEETVSSFCIEFSTAERKMGELKKLVEEGKIKYIGLSEASA 181

Query: 203 DMIRRAHAVHPITALQMEYSLWCRETEDEIIPLCRELGIGIVAYSPLGRGFFGGKAVIES 262
           D IRRAHAVHPITALQMEYSLW R+ EDEIIPLCRELGIG+VAYSPLGRGFFGGKAV ES
Sbjct: 182 DTIRRAHAVHPITALQMEYSLWSRDIEDEIIPLCRELGIGMVAYSPLGRGFFGGKAVAES 241

Query: 263 LPSESLLVKHPRFSKDSLKVNETIYERLVDLAAKHRCTTAQLALAWLLHQGIDIVPVPGT 322
           LPSESLLVKHPRF+K+SL+ NE IY+RL+D+AAKHRC T QLALAWLLHQGIDIVP+PGT
Sbjct: 242 LPSESLLVKHPRFAKESLEENEAIYKRLLDMAAKHRCATVQLALAWLLHQGIDIVPIPGT 301

Query: 323 TKVGNLDSNIGSLSVKLTEKDLKEISDAVPIDEVRGEREYDAFSSYMWNYAGSSSK 365
           TKVGNLDSNI SL V+LT +DLKEI DAVP+DEVRG+REYD FS YMWN+A   ++
Sbjct: 302 TKVGNLDSNIRSLGVELTPEDLKEIGDAVPVDEVRGQREYDVFSKYMWNFADKGAE 357

BLAST of HG10010216 vs. NCBI nr
Match: XP_022138490.1 (perakine reductase-like isoform X1 [Momordica charantia])

HSP 1 Score: 599.4 bits (1544), Expect = 2.1e-167
Identity = 298/368 (80.98%), Postives = 319/368 (86.68%), Query Frame = 0

Query: 23  PQNLSQIPRVKLGSQGLEVSRLGFGCAGLSGLYNAPLTHEGGCRMIKQVFTRGITFFDTS 82
           PQNL QIPRVKLGSQGLEVSRLGFGCAGLSG+YNAPLTH+ GC MIKQVFTRGITFFDTS
Sbjct: 2   PQNLIQIPRVKLGSQGLEVSRLGFGCAGLSGIYNAPLTHKDGCGMIKQVFTRGITFFDTS 61

Query: 83  DLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLGSFQFAVNGTPEYVRKCCEASLER 142
           DLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLG F+F+VNGTPEYVRK CEASLER
Sbjct: 62  DLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLGDFRFSVNGTPEYVRKSCEASLER 121

Query: 143 LQVDHIDLYYQHRIDLSVPIEET--------------MGELKKLVEEGKIKYIGLSEASA 202
           LQVDHIDLYYQHR+D SVPIEET              MGELKKLVEEGKIKYIGLSEASA
Sbjct: 122 LQVDHIDLYYQHRVDPSVPIEETVSSFCIEFSTAERKMGELKKLVEEGKIKYIGLSEASA 181

Query: 203 DMIRRAHAVHPITALQMEYSLWCRETEDEIIPLCRELGIGIVAYSPLGRGFFGGKAVIES 262
           D IRRAHAVHPITALQMEYSLW R+ EDEIIPLCRELGIG+VAYSPLGRGFFGGKAV ES
Sbjct: 182 DTIRRAHAVHPITALQMEYSLWSRDIEDEIIPLCRELGIGMVAYSPLGRGFFGGKAVAES 241

Query: 263 LPSESLLVKHPRFSKDSLKVNETIYERLVDLAAKHRCTTAQLALAWLLHQGIDIVPVP-- 322
           LPSESLLVKHPRF+K+SL+ NE IY+RL+D+AAKHRC T QLALAWLLHQGIDIVP+P  
Sbjct: 242 LPSESLLVKHPRFAKESLEENEAIYKRLLDMAAKHRCATVQLALAWLLHQGIDIVPIPVP 301

Query: 323 ----------GTTKVGNLDSNIGSLSVKLTEKDLKEISDAVPIDEVRGEREYDAFSSYMW 365
                     GTTKVGNLDSNI SL V+LT +DLKEI DAVP+DEVRG+REYD FS YMW
Sbjct: 302 TLCFVFHSGKGTTKVGNLDSNIRSLGVELTPEDLKEIGDAVPVDEVRGQREYDVFSKYMW 361

BLAST of HG10010216 vs. ExPASy Swiss-Prot
Match: C6TBN2 (Probable aldo-keto reductase 1 OS=Glycine max OX=3847 GN=AKR1 PE=2 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 4.8e-130
Identity = 220/338 (65.09%), Postives = 271/338 (80.18%), Query Frame = 0

Query: 27  SQIPRVKLGSQGLEVSRLGFGCAGLSGLYNAPLTHEGGCRMIKQVFTRGITFFDTSDLYG 86
           +QI  VKLG+QG EVS+LGFGC GL+G YN PL  + G  +IK  F++GITFFDT+D+YG
Sbjct: 4   AQIQPVKLGTQGFEVSKLGFGCMGLTGAYNDPLQEQDGISVIKYAFSKGITFFDTADVYG 63

Query: 87  ADHDNEIMVGKALKQLPREKIQLATKFGIVPLGSFQFAVNGTPEYVRKCCEASLERLQVD 146
           A + NE++VGKALKQLPREKIQ+ATKFGI   G     + G+PEYVR CCE  L+RL V+
Sbjct: 64  A-NANELLVGKALKQLPREKIQIATKFGIASRGFPDMKIEGSPEYVRSCCETGLKRLDVE 123

Query: 147 HIDLYYQHRIDLSVPIEETMGELKKLVEEGKIKYIGLSEASADMIRRAHAVHPITALQME 206
           +IDLYYQHR+D SVPIEET+GELKKLVEEGK+KYIGLSEAS D IRRAHA+HPITA+Q+E
Sbjct: 124 YIDLYYQHRVDTSVPIEETVGELKKLVEEGKVKYIGLSEASPDTIRRAHAIHPITAVQIE 183

Query: 207 YSLWCRETEDEIIPLCRELGIGIVAYSPLGRGFFGGKAVIESLPSESLLVKHPRFSKDSL 266
           +SLW R+ E+EI+PLCRELGIGIV YSPLGRGFFGGK V+E++P+ S L  HPRF  ++L
Sbjct: 184 WSLWTRDIEEEIVPLCRELGIGIVPYSPLGRGFFGGKGVVENVPTNSSLKAHPRFQAENL 243

Query: 267 KVNETIYERLVDLAAKHRCTTAQLALAWLLHQGIDIVPVPGTTKVGNLDSNIGSLSVKLT 326
             N+ IYER+  LA KH+ T AQLALAW+L QG D+VP+PGTTK+ NLD NIG+L+VKL+
Sbjct: 244 DKNKNIYERIEGLAKKHQATPAQLALAWVLQQGEDVVPIPGTTKIKNLDQNIGALAVKLS 303

Query: 327 EKDLKEISDAVPIDEVRGEREYDAFSSYMWNYAGSSSK 365
           EKDL+EI +AVPI +V G R Y+    + W YA +  K
Sbjct: 304 EKDLREIFEAVPIGDVAGGRYYNGLDHFSWKYANTPPK 340

BLAST of HG10010216 vs. ExPASy Swiss-Prot
Match: Q3L181 (Perakine reductase OS=Rauvolfia serpentina OX=4060 GN=PR PE=1 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 3.5e-128
Identity = 219/333 (65.77%), Postives = 265/333 (79.58%), Query Frame = 0

Query: 29  IPRVKLGSQGLEVSRLGFGCAGLSGLYNAPLTHEGGCRMIKQVFTRGITFFDTSDLYGAD 88
           +PRVKLG+QGLEVS+LGFGC GLSG YN  L  E G  +IK+ F  GITFFDTSD+YG +
Sbjct: 1   MPRVKLGTQGLEVSKLGFGCMGLSGDYNDALPEEQGIAVIKEAFNCGITFFDTSDIYGEN 60

Query: 89  HDNEIMVGKALKQLPREKIQLATKFGIVPLGSFQFAVNGTPEYVRKCCEASLERLQVDHI 148
             NE ++GKALKQLPREKIQ+ TKFGI  +G       GTP+YVR CCEASL+RL VD+I
Sbjct: 61  GSNEELLGKALKQLPREKIQVGTKFGIHEIGFSGVKAKGTPDYVRSCCEASLKRLDVDYI 120

Query: 149 DLYYQHRIDLSVPIEETMGELKKLVEEGKIKYIGLSEASADMIRRAHAVHPITALQMEYS 208
           DL+Y HRID +VPIE TMGELKKLVEEGKIKY+GLSEAS D IRRAHAVHP+TALQ+EYS
Sbjct: 121 DLFYIHRIDTTVPIEITMGELKKLVEEGKIKYVGLSEASPDTIRRAHAVHPVTALQIEYS 180

Query: 209 LWCRETEDEIIPLCRELGIGIVAYSPLGRGFFGGKAVIESLPSESLLVKHPRFSKDSLKV 268
           LW R+ EDEI+PLCR+LGIGIV YSP+GRG F GKA+ ESLP  S+L  HPRF  ++L+ 
Sbjct: 181 LWTRDIEDEIVPLCRQLGIGIVPYSPIGRGLFAGKAIKESLPENSVLTSHPRFVGENLEK 240

Query: 269 NETIYERLVDLAAKHRCTTAQLALAWLLHQGIDIVPVPGTTKVGNLDSNIGSLSVKLTEK 328
           N+ IY R+  L+ KH CT  QLALAW+LHQG D+VP+PGTTK+ NL +N+G+L VKLT++
Sbjct: 241 NKQIYYRIEALSQKHGCTPVQLALAWVLHQGEDVVPIPGTTKIKNLHNNVGALKVKLTKE 300

Query: 329 DLKEISDAVPIDEVRGEREYDAFSSYMWNYAGS 362
           DLKEISDAVP+DEV GE  ++  +   W +A +
Sbjct: 301 DLKEISDAVPLDEVAGESIHEVIAVTNWKFANT 333

BLAST of HG10010216 vs. ExPASy Swiss-Prot
Match: A2XRZ0 (Probable aldo-keto reductase 2 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_15387 PE=3 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 2.1e-101
Identity = 185/329 (56.23%), Postives = 233/329 (70.82%), Query Frame = 0

Query: 29  IPRVKLGSQGLEVSRLGFGCAGLSGLYNAPLTHEGGCRMIKQVFTRGITFFDTSDLYGAD 88
           + R+KLGSQGLEVS  G GC G+S  Y  P        +I      G+T  DTSD+YG  
Sbjct: 11  VRRMKLGSQGLEVSAQGLGCMGMSAFYGPPKPEPDMVALIHHAVAAGVTLLDTSDIYG-P 70

Query: 89  HDNEIMVGKALKQLPREKIQLATKFGIVPLGSFQFAVNGTPEYVRKCCEASLERLQVDHI 148
           H NE+++GKAL+   R+K++LATKFGI      +  V G P YVR  CE SL RL VD I
Sbjct: 71  HTNELLLGKALQGGVRDKVELATKFGIA-FEDGKRGVRGDPAYVRAACEGSLRRLGVDSI 130

Query: 149 DLYYQHRIDLSVPIEETMGELKKLVEEGKIKYIGLSEASADMIRRAHAVHPITALQMEYS 208
           DLYYQHR+D  VPIE T+GELKKLVEEGKIKYIGLSEASA  IRRAHAVHPITA+Q+E+S
Sbjct: 131 DLYYQHRVDKKVPIEVTIGELKKLVEEGKIKYIGLSEASASTIRRAHAVHPITAVQLEWS 190

Query: 209 LWCRETEDEIIPLCRELGIGIVAYSPLGRGFF-GGKAVIESLPSESLLVKHPRFSKDSLK 268
           LW R+ E++IIP CRELGIGIVAYSPLGRGFF  G  ++ESL  +      PRF +++L+
Sbjct: 191 LWSRDVEEDIIPTCRELGIGIVAYSPLGRGFFSAGAKLVESLSDQDFRKHIPRFQQENLE 250

Query: 269 VNETIYERLVDLAAKHRCTTAQLALAWLLHQGIDIVPVPGTTKVGNLDSNIGSLSVKLTE 328
            N  I+ER+  +AA+  CT +QLALAW+ HQG D+ P+PGTTK+ NL+ NIG+LSVKLT 
Sbjct: 251 KNAEIFERVNAMAARKGCTPSQLALAWVHHQGSDVCPIPGTTKIENLNQNIGALSVKLTP 310

Query: 329 KDLKEISDAVPIDEVRGEREYDAFSSYMW 357
           +++ E+      D+VRG+R   A ++  W
Sbjct: 311 EEMAELESYASTDDVRGDRYPQAMANTTW 337

BLAST of HG10010216 vs. ExPASy Swiss-Prot
Match: Q7XT99 (Probable aldo-keto reductase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0338000 PE=2 SV=2)

HSP 1 Score: 369.8 bits (948), Expect = 3.6e-101
Identity = 185/329 (56.23%), Postives = 233/329 (70.82%), Query Frame = 0

Query: 29  IPRVKLGSQGLEVSRLGFGCAGLSGLYNAPLTHEGGCRMIKQVFTRGITFFDTSDLYGAD 88
           + R+KLGSQGLEVS  G GC G+S  Y  P        +I      G+T  DTSD+YG  
Sbjct: 11  VRRMKLGSQGLEVSAQGLGCMGMSAFYGPPKPEPDMVALIHHAVAAGVTLLDTSDIYG-P 70

Query: 89  HDNEIMVGKALKQLPREKIQLATKFGIVPLGSFQFAVNGTPEYVRKCCEASLERLQVDHI 148
           H NE+++GKAL+   R+K++LATKFGI      +  V G P YVR  CE SL RL VD I
Sbjct: 71  HTNELLLGKALQGGVRDKVELATKFGIA-FEDGKRDVRGDPAYVRAACEGSLRRLGVDSI 130

Query: 149 DLYYQHRIDLSVPIEETMGELKKLVEEGKIKYIGLSEASADMIRRAHAVHPITALQMEYS 208
           DLYYQHR+D  VPIE T+GELKKLVEEGKIKYIGLSEASA  IRRAHAVHPITA+Q+E+S
Sbjct: 131 DLYYQHRVDKKVPIEVTIGELKKLVEEGKIKYIGLSEASASTIRRAHAVHPITAVQLEWS 190

Query: 209 LWCRETEDEIIPLCRELGIGIVAYSPLGRGFF-GGKAVIESLPSESLLVKHPRFSKDSLK 268
           LW R+ E++IIP CRELGIGIVAYSPLGRGFF  G  ++ESL  +      PRF +++L+
Sbjct: 191 LWSRDVEEDIIPTCRELGIGIVAYSPLGRGFFSAGAKLVESLSDQDFRKHIPRFQQENLE 250

Query: 269 VNETIYERLVDLAAKHRCTTAQLALAWLLHQGIDIVPVPGTTKVGNLDSNIGSLSVKLTE 328
            N  I+ER+  +AA+  CT +QLALAW+ HQG D+ P+PGTTK+ NL+ NIG+LSVKLT 
Sbjct: 251 KNAEIFERVNAMAARKGCTPSQLALAWVHHQGSDVCPIPGTTKIENLNQNIGALSVKLTP 310

Query: 329 KDLKEISDAVPIDEVRGEREYDAFSSYMW 357
           +++ E+      D+VRG+R   A ++  W
Sbjct: 311 EEMAELESYASTDDVRGDRYPQAMANTTW 337

BLAST of HG10010216 vs. ExPASy Swiss-Prot
Match: F4HPY8 (Probable aldo-keto reductase 6 OS=Arabidopsis thaliana OX=3702 GN=At1g60750 PE=3 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 2.0e-99
Identity = 184/322 (57.14%), Postives = 230/322 (71.43%), Query Frame = 0

Query: 28  QIPRVKLGSQGLEVSRLGFGCAGLSGLYNAPLTHEGGCRMIKQVFTRGITFFDTSDLYGA 87
           Q+ R+KLGSQGLEVS  G GC GLS  Y AP        +++     G+TF DTSD+YG 
Sbjct: 7   QVRRMKLGSQGLEVSAQGLGCMGLSDFYGAPTPETNAVALLRHAINAGVTFLDTSDIYGP 66

Query: 88  DHDNEIMVGKALKQLPREKIQLATKFGIVPLGSFQFAVNGTPEYVRKCCEASLERLQVDH 147
           +  NE+++GKALK   R+K++LATKFGI      +F   G PEYVR  CEASL+RL V  
Sbjct: 67  E-TNELLLGKALKDGLRDKVELATKFGITASEDGKFGFRGDPEYVRIACEASLKRLGVTC 126

Query: 148 IDLYYQHRIDLSVPIEETMGELKKLVEEGKIKYIGLSEASADMIRRAHAVHPITALQMEY 207
           IDLYYQHRID ++PIE T+GELKKLVEEGKIKYIGLSEASA  IRRAHAVHPITA+Q+E+
Sbjct: 127 IDLYYQHRIDTTLPIEITIGELKKLVEEGKIKYIGLSEASASTIRRAHAVHPITAVQIEW 186

Query: 208 SLWCRETEDEIIPLCRELGIGIVAYSPLGRGFFGGKAVIESLPSESLLVKHPRFSKDSLK 267
           SLW R+ E++IIP CRELGIGIVAYSPLGRGF G                 PRF +++L+
Sbjct: 187 SLWSRDVEEDIIPTCRELGIGIVAYSPLGRGFLG----------------LPRFQQENLE 246

Query: 268 VNETIYERLVDLAAKHRCTTAQLALAWLLHQGIDIVPVPGTTKVGNLDSNIGSLSVKLTE 327
            N+ +YE++  +A K  CT AQLALAW+ HQG D+ P+PGT+K+ NL+ NIG+LSVKLT 
Sbjct: 247 NNKILYEKVQAMATKKSCTPAQLALAWVHHQGDDVCPIPGTSKIQNLNQNIGALSVKLTP 306

Query: 328 KDLKEISDAVPIDEVRGEREYD 350
           +++ E+      D V+GER YD
Sbjct: 307 EEMVELEAIAQPDFVKGER-YD 310

BLAST of HG10010216 vs. ExPASy TrEMBL
Match: A0A6J1CA99 (perakine reductase-like isoform X5 OS=Momordica charantia OX=3673 GN=LOC111009653 PE=4 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 2.8e-173
Identity = 298/342 (87.13%), Postives = 319/342 (93.27%), Query Frame = 0

Query: 23  PQNLSQIPRVKLGSQGLEVSRLGFGCAGLSGLYNAPLTHEGGCRMIKQVFTRGITFFDTS 82
           PQNL QIPRVKLGSQGLEVSRLGFGCAGLSG+YNAPLTH+ GC MIKQVFTRGITFFDTS
Sbjct: 2   PQNLIQIPRVKLGSQGLEVSRLGFGCAGLSGIYNAPLTHKDGCGMIKQVFTRGITFFDTS 61

Query: 83  DLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLGSFQFAVNGTPEYVRKCCEASLER 142
           DLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLG F+F+VNGTPEYVRK CEASLER
Sbjct: 62  DLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLGDFRFSVNGTPEYVRKSCEASLER 121

Query: 143 LQVDHIDLYYQHRIDLSVPIEETMGELKKLVEEGKIKYIGLSEASADMIRRAHAVHPITA 202
           LQVDHIDLYYQHR+D SVPIEETMGELKKLVEEGKIKYIGLSEASAD IRRAHAVHPITA
Sbjct: 122 LQVDHIDLYYQHRVDPSVPIEETMGELKKLVEEGKIKYIGLSEASADTIRRAHAVHPITA 181

Query: 203 LQMEYSLWCRETEDEIIPLCRELGIGIVAYSPLGRGFFGGKAVIESLPSESLLVKHPRFS 262
           LQMEYSLW R+ EDEIIPLCRELGIG+VAYSPLGRGFFGGKAV ESLPSESLLVKHPRF+
Sbjct: 182 LQMEYSLWSRDIEDEIIPLCRELGIGMVAYSPLGRGFFGGKAVAESLPSESLLVKHPRFA 241

Query: 263 KDSLKVNETIYERLVDLAAKHRCTTAQLALAWLLHQGIDIVPVPGTTKVGNLDSNIGSLS 322
           K+SL+ NE IY+RL+D+AAKHRC T QLALAWLLHQGIDIVP+PGTTKVGNLDSNI SL 
Sbjct: 242 KESLEENEAIYKRLLDMAAKHRCATVQLALAWLLHQGIDIVPIPGTTKVGNLDSNIRSLG 301

Query: 323 VKLTEKDLKEISDAVPIDEVRGEREYDAFSSYMWNYAGSSSK 365
           V+LT +DLKEI DAVP+DEVRG+REYD FS YMWN+A   ++
Sbjct: 302 VELTPEDLKEIGDAVPVDEVRGQREYDVFSKYMWNFADKGAE 343

BLAST of HG10010216 vs. ExPASy TrEMBL
Match: A0A6J1CD50 (perakine reductase-like isoform X4 OS=Momordica charantia OX=3673 GN=LOC111009653 PE=4 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 1.3e-170
Identity = 298/354 (84.18%), Postives = 319/354 (90.11%), Query Frame = 0

Query: 23  PQNLSQIPRVKLGSQGLEVSRLGFGCAGLSGLYNAPLTHEGGCRMIKQVFTRGITFFDTS 82
           PQNL QIPRVKLGSQGLEVSRLGFGCAGLSG+YNAPLTH+ GC MIKQVFTRGITFFDTS
Sbjct: 2   PQNLIQIPRVKLGSQGLEVSRLGFGCAGLSGIYNAPLTHKDGCGMIKQVFTRGITFFDTS 61

Query: 83  DLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLGSFQFAVNGTPEYVRKCCEASLER 142
           DLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLG F+F+VNGTPEYVRK CEASLER
Sbjct: 62  DLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLGDFRFSVNGTPEYVRKSCEASLER 121

Query: 143 LQVDHIDLYYQHRIDLSVPIEETMGELKKLVEEGKIKYIGLSEASADMIRRAHAVHPITA 202
           LQVDHIDLYYQHR+D SVPIEETMGELKKLVEEGKIKYIGLSEASAD IRRAHAVHPITA
Sbjct: 122 LQVDHIDLYYQHRVDPSVPIEETMGELKKLVEEGKIKYIGLSEASADTIRRAHAVHPITA 181

Query: 203 LQMEYSLWCRETEDEIIPLCRELGIGIVAYSPLGRGFFGGKAVIESLPSESLLVKHPRFS 262
           LQMEYSLW R+ EDEIIPLCRELGIG+VAYSPLGRGFFGGKAV ESLPSESLLVKHPRF+
Sbjct: 182 LQMEYSLWSRDIEDEIIPLCRELGIGMVAYSPLGRGFFGGKAVAESLPSESLLVKHPRFA 241

Query: 263 KDSLKVNETIYERLVDLAAKHRCTTAQLALAWLLHQGIDIVPVP------------GTTK 322
           K+SL+ NE IY+RL+D+AAKHRC T QLALAWLLHQGIDIVP+P            GTTK
Sbjct: 242 KESLEENEAIYKRLLDMAAKHRCATVQLALAWLLHQGIDIVPIPVPTLCFVFHSGKGTTK 301

Query: 323 VGNLDSNIGSLSVKLTEKDLKEISDAVPIDEVRGEREYDAFSSYMWNYAGSSSK 365
           VGNLDSNI SL V+LT +DLKEI DAVP+DEVRG+REYD FS YMWN+A   ++
Sbjct: 302 VGNLDSNIRSLGVELTPEDLKEIGDAVPVDEVRGQREYDVFSKYMWNFADKGAE 355

BLAST of HG10010216 vs. ExPASy TrEMBL
Match: A0A6J1CB89 (perakine reductase-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111009653 PE=4 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 2.2e-170
Identity = 298/356 (83.71%), Postives = 319/356 (89.61%), Query Frame = 0

Query: 23  PQNLSQIPRVKLGSQGLEVSRLGFGCAGLSGLYNAPLTHEGGCRMIKQVFTRGITFFDTS 82
           PQNL QIPRVKLGSQGLEVSRLGFGCAGLSG+YNAPLTH+ GC MIKQVFTRGITFFDTS
Sbjct: 2   PQNLIQIPRVKLGSQGLEVSRLGFGCAGLSGIYNAPLTHKDGCGMIKQVFTRGITFFDTS 61

Query: 83  DLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLGSFQFAVNGTPEYVRKCCEASLER 142
           DLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLG F+F+VNGTPEYVRK CEASLER
Sbjct: 62  DLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLGDFRFSVNGTPEYVRKSCEASLER 121

Query: 143 LQVDHIDLYYQHRIDLSVPIEET--------------MGELKKLVEEGKIKYIGLSEASA 202
           LQVDHIDLYYQHR+D SVPIEET              MGELKKLVEEGKIKYIGLSEASA
Sbjct: 122 LQVDHIDLYYQHRVDPSVPIEETVSSFCIEFSTAERKMGELKKLVEEGKIKYIGLSEASA 181

Query: 203 DMIRRAHAVHPITALQMEYSLWCRETEDEIIPLCRELGIGIVAYSPLGRGFFGGKAVIES 262
           D IRRAHAVHPITALQMEYSLW R+ EDEIIPLCRELGIG+VAYSPLGRGFFGGKAV ES
Sbjct: 182 DTIRRAHAVHPITALQMEYSLWSRDIEDEIIPLCRELGIGMVAYSPLGRGFFGGKAVAES 241

Query: 263 LPSESLLVKHPRFSKDSLKVNETIYERLVDLAAKHRCTTAQLALAWLLHQGIDIVPVPGT 322
           LPSESLLVKHPRF+K+SL+ NE IY+RL+D+AAKHRC T QLALAWLLHQGIDIVP+PGT
Sbjct: 242 LPSESLLVKHPRFAKESLEENEAIYKRLLDMAAKHRCATVQLALAWLLHQGIDIVPIPGT 301

Query: 323 TKVGNLDSNIGSLSVKLTEKDLKEISDAVPIDEVRGEREYDAFSSYMWNYAGSSSK 365
           TKVGNLDSNI SL V+LT +DLKEI DAVP+DEVRG+REYD FS YMWN+A   ++
Sbjct: 302 TKVGNLDSNIRSLGVELTPEDLKEIGDAVPVDEVRGQREYDVFSKYMWNFADKGAE 357

BLAST of HG10010216 vs. ExPASy TrEMBL
Match: A0A6J1C9K6 (perakine reductase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111009653 PE=4 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 1.0e-167
Identity = 298/368 (80.98%), Postives = 319/368 (86.68%), Query Frame = 0

Query: 23  PQNLSQIPRVKLGSQGLEVSRLGFGCAGLSGLYNAPLTHEGGCRMIKQVFTRGITFFDTS 82
           PQNL QIPRVKLGSQGLEVSRLGFGCAGLSG+YNAPLTH+ GC MIKQVFTRGITFFDTS
Sbjct: 2   PQNLIQIPRVKLGSQGLEVSRLGFGCAGLSGIYNAPLTHKDGCGMIKQVFTRGITFFDTS 61

Query: 83  DLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLGSFQFAVNGTPEYVRKCCEASLER 142
           DLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLG F+F+VNGTPEYVRK CEASLER
Sbjct: 62  DLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLGDFRFSVNGTPEYVRKSCEASLER 121

Query: 143 LQVDHIDLYYQHRIDLSVPIEET--------------MGELKKLVEEGKIKYIGLSEASA 202
           LQVDHIDLYYQHR+D SVPIEET              MGELKKLVEEGKIKYIGLSEASA
Sbjct: 122 LQVDHIDLYYQHRVDPSVPIEETVSSFCIEFSTAERKMGELKKLVEEGKIKYIGLSEASA 181

Query: 203 DMIRRAHAVHPITALQMEYSLWCRETEDEIIPLCRELGIGIVAYSPLGRGFFGGKAVIES 262
           D IRRAHAVHPITALQMEYSLW R+ EDEIIPLCRELGIG+VAYSPLGRGFFGGKAV ES
Sbjct: 182 DTIRRAHAVHPITALQMEYSLWSRDIEDEIIPLCRELGIGMVAYSPLGRGFFGGKAVAES 241

Query: 263 LPSESLLVKHPRFSKDSLKVNETIYERLVDLAAKHRCTTAQLALAWLLHQGIDIVPVP-- 322
           LPSESLLVKHPRF+K+SL+ NE IY+RL+D+AAKHRC T QLALAWLLHQGIDIVP+P  
Sbjct: 242 LPSESLLVKHPRFAKESLEENEAIYKRLLDMAAKHRCATVQLALAWLLHQGIDIVPIPVP 301

Query: 323 ----------GTTKVGNLDSNIGSLSVKLTEKDLKEISDAVPIDEVRGEREYDAFSSYMW 365
                     GTTKVGNLDSNI SL V+LT +DLKEI DAVP+DEVRG+REYD FS YMW
Sbjct: 302 TLCFVFHSGKGTTKVGNLDSNIRSLGVELTPEDLKEIGDAVPVDEVRGQREYDVFSKYMW 361

BLAST of HG10010216 vs. ExPASy TrEMBL
Match: A0A6J1C9V6 (perakine reductase-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111009653 PE=4 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 3.1e-164
Identity = 295/368 (80.16%), Postives = 316/368 (85.87%), Query Frame = 0

Query: 23  PQNLSQIPRVKLGSQGLEVSRLGFGCAGLSGLYNAPLTHEGGCRMIKQVFTRGITFFDTS 82
           PQNL QIPRVKLGSQGLEVSRLGFGCAGLSG+YNAPLTH+ GC MIKQVFTRGITFFDTS
Sbjct: 2   PQNLIQIPRVKLGSQGLEVSRLGFGCAGLSGIYNAPLTHKDGCGMIKQVFTRGITFFDTS 61

Query: 83  DLYGADHDNEIMVGKALKQLPREKIQLATKFGIVPLGSFQFAVNGTPEYVRKCCEASLER 142
           DLYGADHDNEIM   ALKQLPREKIQLATKFGIVPLG F+F+VNGTPEYVRK CEASLER
Sbjct: 62  DLYGADHDNEIM---ALKQLPREKIQLATKFGIVPLGDFRFSVNGTPEYVRKSCEASLER 121

Query: 143 LQVDHIDLYYQHRIDLSVPIEET--------------MGELKKLVEEGKIKYIGLSEASA 202
           LQVDHIDLYYQHR+D SVPIEET              MGELKKLVEEGKIKYIGLSEASA
Sbjct: 122 LQVDHIDLYYQHRVDPSVPIEETVSSFCIEFSTAERKMGELKKLVEEGKIKYIGLSEASA 181

Query: 203 DMIRRAHAVHPITALQMEYSLWCRETEDEIIPLCRELGIGIVAYSPLGRGFFGGKAVIES 262
           D IRRAHAVHPITALQMEYSLW R+ EDEIIPLCRELGIG+VAYSPLGRGFFGGKAV ES
Sbjct: 182 DTIRRAHAVHPITALQMEYSLWSRDIEDEIIPLCRELGIGMVAYSPLGRGFFGGKAVAES 241

Query: 263 LPSESLLVKHPRFSKDSLKVNETIYERLVDLAAKHRCTTAQLALAWLLHQGIDIVPVP-- 322
           LPSESLLVKHPRF+K+SL+ NE IY+RL+D+AAKHRC T QLALAWLLHQGIDIVP+P  
Sbjct: 242 LPSESLLVKHPRFAKESLEENEAIYKRLLDMAAKHRCATVQLALAWLLHQGIDIVPIPVP 301

Query: 323 ----------GTTKVGNLDSNIGSLSVKLTEKDLKEISDAVPIDEVRGEREYDAFSSYMW 365
                     GTTKVGNLDSNI SL V+LT +DLKEI DAVP+DEVRG+REYD FS YMW
Sbjct: 302 TLCFVFHSGKGTTKVGNLDSNIRSLGVELTPEDLKEIGDAVPVDEVRGQREYDVFSKYMW 361

BLAST of HG10010216 vs. TAIR 10
Match: AT1G60750.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 364.0 bits (933), Expect = 1.4e-100
Identity = 184/322 (57.14%), Postives = 230/322 (71.43%), Query Frame = 0

Query: 28  QIPRVKLGSQGLEVSRLGFGCAGLSGLYNAPLTHEGGCRMIKQVFTRGITFFDTSDLYGA 87
           Q+ R+KLGSQGLEVS  G GC GLS  Y AP        +++     G+TF DTSD+YG 
Sbjct: 7   QVRRMKLGSQGLEVSAQGLGCMGLSDFYGAPTPETNAVALLRHAINAGVTFLDTSDIYGP 66

Query: 88  DHDNEIMVGKALKQLPREKIQLATKFGIVPLGSFQFAVNGTPEYVRKCCEASLERLQVDH 147
           +  NE+++GKALK   R+K++LATKFGI      +F   G PEYVR  CEASL+RL V  
Sbjct: 67  E-TNELLLGKALKDGLRDKVELATKFGITASEDGKFGFRGDPEYVRIACEASLKRLGVTC 126

Query: 148 IDLYYQHRIDLSVPIEETMGELKKLVEEGKIKYIGLSEASADMIRRAHAVHPITALQMEY 207
           IDLYYQHRID ++PIE T+GELKKLVEEGKIKYIGLSEASA  IRRAHAVHPITA+Q+E+
Sbjct: 127 IDLYYQHRIDTTLPIEITIGELKKLVEEGKIKYIGLSEASASTIRRAHAVHPITAVQIEW 186

Query: 208 SLWCRETEDEIIPLCRELGIGIVAYSPLGRGFFGGKAVIESLPSESLLVKHPRFSKDSLK 267
           SLW R+ E++IIP CRELGIGIVAYSPLGRGF G                 PRF +++L+
Sbjct: 187 SLWSRDVEEDIIPTCRELGIGIVAYSPLGRGFLG----------------LPRFQQENLE 246

Query: 268 VNETIYERLVDLAAKHRCTTAQLALAWLLHQGIDIVPVPGTTKVGNLDSNIGSLSVKLTE 327
            N+ +YE++  +A K  CT AQLALAW+ HQG D+ P+PGT+K+ NL+ NIG+LSVKLT 
Sbjct: 247 NNKILYEKVQAMATKKSCTPAQLALAWVHHQGDDVCPIPGTSKIQNLNQNIGALSVKLTP 306

Query: 328 KDLKEISDAVPIDEVRGEREYD 350
           +++ E+      D V+GER YD
Sbjct: 307 EEMVELEAIAQPDFVKGER-YD 310

BLAST of HG10010216 vs. TAIR 10
Match: AT1G60710.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 363.6 bits (932), Expect = 1.8e-100
Identity = 183/319 (57.37%), Postives = 227/319 (71.16%), Query Frame = 0

Query: 29  IPRVKLGSQGLEVSRLGFGCAGLSGLYNAPLTHEGGCRMIKQVFTRGITFFDTSDLYGAD 88
           + R+KLGSQGLEVS  G GC GLS  Y AP        +I      G+T  DTSD+YG +
Sbjct: 7   VRRMKLGSQGLEVSAQGLGCMGLSAFYGAPKPENEAIALIHHAIHSGVTLLDTSDIYGPE 66

Query: 89  HDNEIMVGKALKQLPREKIQLATKFGIVPLGSFQFAVNGTPEYVRKCCEASLERLQVDHI 148
             NE+++GKALK   REK++LATKFGI      +  V G PEYVR  CEASL+RL +  I
Sbjct: 67  -TNEVLLGKALKDGVREKVELATKFGI-SYAEGKREVRGDPEYVRAACEASLKRLDIACI 126

Query: 149 DLYYQHRIDLSVPIEETMGELKKLVEEGKIKYIGLSEASADMIRRAHAVHPITALQMEYS 208
           DLYYQHR+D  VPIE TMGELKKLVEEGKIKYIGLSEASA  IRRAHAVHPITA+Q+E+S
Sbjct: 127 DLYYQHRVDTRVPIEITMGELKKLVEEGKIKYIGLSEASASTIRRAHAVHPITAVQIEWS 186

Query: 209 LWCRETEDEIIPLCRELGIGIVAYSPLGRGFF-GGKAVIESLPSESLLVKHPRFSKDSLK 268
           LW R+ E+EIIP CRELGIGIVAYSPLGRGFF  G  ++E+L  +      PRF +++L 
Sbjct: 187 LWTRDVEEEIIPTCRELGIGIVAYSPLGRGFFASGPKLVENLEKDDFRKALPRFQEENLD 246

Query: 269 VNETIYERLVDLAAKHRCTTAQLALAWLLHQGIDIVPVPGTTKVGNLDSNIGSLSVKLTE 328
            N+ +YE++  ++ K  CT  QLALAW+ HQG D+ P+PGTTK+ NL  NIG+LSVKLT 
Sbjct: 247 HNKIVYEKVCAISEKKGCTPGQLALAWVHHQGDDVCPIPGTTKIENLKQNIGALSVKLTP 306

Query: 329 KDLKEISDAVPIDEVRGER 347
           +++ E+        V+G+R
Sbjct: 307 EEMTELEAIAQPGFVKGDR 323

BLAST of HG10010216 vs. TAIR 10
Match: AT1G60690.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 361.3 bits (926), Expect = 9.1e-100
Identity = 181/320 (56.56%), Postives = 228/320 (71.25%), Query Frame = 0

Query: 28  QIPRVKLGSQGLEVSRLGFGCAGLSGLYNAPLTHEGGCRMIKQVFTRGITFFDTSDLYGA 87
           ++ R+KLGSQGLEVS  G GC GL+G Y A         +I      G+TF DTSD+YG 
Sbjct: 6   RVRRIKLGSQGLEVSAQGLGCMGLTGHYGASKPETEAIALIHHAIHSGVTFLDTSDMYGP 65

Query: 88  DHDNEIMVGKALKQLPREKIQLATKFGIVPLGSFQFAVNGTPEYVRKCCEASLERLQVDH 147
           +  NEI++GKALK   REK++LATKFGI         + G P YVR  CEASL+RL V  
Sbjct: 66  E-TNEILLGKALKDGVREKVELATKFGI-SYAEGNREIKGDPAYVRAACEASLKRLDVTC 125

Query: 148 IDLYYQHRIDLSVPIEETMGELKKLVEEGKIKYIGLSEASADMIRRAHAVHPITALQMEY 207
           IDLYYQHRID  VPIE TMGELKKL+EEGKIKYIGLSEASA  IRRAH VHPITA+Q+E+
Sbjct: 126 IDLYYQHRIDTRVPIEITMGELKKLIEEGKIKYIGLSEASASTIRRAHTVHPITAVQLEW 185

Query: 208 SLWCRETEDEIIPLCRELGIGIVAYSPLGRGFF-GGKAVIESLPSESLLVKHPRFSKDSL 267
           SLW R+ E+EI+P CRELGIGIV+YSPLGRGFF  G  ++E+L +       PRF +++L
Sbjct: 186 SLWTRDVEEEIVPTCRELGIGIVSYSPLGRGFFASGPKLVENLDNNDFRKALPRFQQENL 245

Query: 268 KVNETIYERLVDLAAKHRCTTAQLALAWLLHQGIDIVPVPGTTKVGNLDSNIGSLSVKLT 327
             N+ +YE++  ++ K  CT AQLALAW+ HQG D+ P+PGTTK+ NL+ NI +LSVKLT
Sbjct: 246 DHNKILYEKVSAMSEKKGCTPAQLALAWVHHQGDDVCPIPGTTKIENLNQNIRALSVKLT 305

Query: 328 EKDLKEISDAVPIDEVRGER 347
            +++ E+      + V+GER
Sbjct: 306 PEEMSELETIAQPESVKGER 323

BLAST of HG10010216 vs. TAIR 10
Match: AT1G60680.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 360.9 bits (925), Expect = 1.2e-99
Identity = 180/328 (54.88%), Postives = 233/328 (71.04%), Query Frame = 0

Query: 28  QIPRVKLGSQGLEVSRLGFGCAGLSGLYNAPLTHEGGCRMIKQVFTRGITFFDTSDLYGA 87
           ++ R+KLGSQGLEVS  G GC  LS  Y AP        ++      G+TFFDTSD+YG 
Sbjct: 6   RVRRMKLGSQGLEVSAQGLGCMALSARYGAPKPETDAIALLHHAINSGVTFFDTSDMYGP 65

Query: 88  DHDNEIMVGKALKQLPREKIQLATKFGIVPLGSFQFAVNGTPEYVRKCCEASLERLQVDH 147
           +  NE+++GKALK   +EK++LATKFG   +      V G PEYVR  CEASL+RL +  
Sbjct: 66  E-TNELLLGKALKDGVKEKVELATKFGFFIVEGEISEVRGDPEYVRAACEASLKRLDIAC 125

Query: 148 IDLYYQHRIDLSVPIEETMGELKKLVEEGKIKYIGLSEASADMIRRAHAVHPITALQMEY 207
           IDLYYQHRID  VPIE TM ELKKLVEEGKIKYIGLSEASA  IRRAHAVHPITA+Q+E+
Sbjct: 126 IDLYYQHRIDTRVPIEITMRELKKLVEEGKIKYIGLSEASASTIRRAHAVHPITAVQIEW 185

Query: 208 SLWCRETEDEIIPLCRELGIGIVAYSPLGRGFF-GGKAVIESLPSESLLVKHPRFSKDSL 267
           SLW R+ E++IIP+CRELGIGIVAYSPLGRGF   G  + E+L ++      PRF ++++
Sbjct: 186 SLWSRDAEEDIIPICRELGIGIVAYSPLGRGFLAAGPKLAENLENDDFRKTLPRFQQENV 245

Query: 268 KVNETIYERLVDLAAKHRCTTAQLALAWLLHQGIDIVPVPGTTKVGNLDSNIGSLSVKLT 327
             N+ ++E++  +A K  CT AQLALAW+ HQG D+ P+PGTTK+ NL+ NI +LSVKLT
Sbjct: 246 DHNKILFEKVSAMAEKKGCTPAQLALAWVHHQGDDVCPIPGTTKIENLNQNIRALSVKLT 305

Query: 328 EKDLKEISDAVPIDEVRGEREYDAFSSY 355
            +++ E+      + V+GER   + S++
Sbjct: 306 PEEISELDSLAKPESVKGERYMASMSTF 332

BLAST of HG10010216 vs. TAIR 10
Match: AT1G60730.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 357.8 bits (917), Expect = 1.0e-98
Identity = 182/328 (55.49%), Postives = 231/328 (70.43%), Query Frame = 0

Query: 29  IPRVKLGSQGLEVSRLGFGCAGLSGLYNAPLTHEGGCRMIKQVFTRGITFFDTSDLYGAD 88
           + R+KLGSQGLEVS  G GC GLS  Y  P        +I      G+TF DTSD+YG +
Sbjct: 7   VRRIKLGSQGLEVSAQGLGCMGLSAFYGTPKPETEAIALIHHAIHSGVTFLDTSDIYGPE 66

Query: 89  HDNEIMVGKALKQLPREKIQLATKFGI-VPLGSFQFAVNGTPEYVRKCCEASLERLQVDH 148
             NE+++ KALK   REK++LATK+GI    G  +F   G P YVR  CEASL R+ V  
Sbjct: 67  -TNELLLSKALKDGVREKVELATKYGIRYAEGKVEF--KGDPAYVRAACEASLMRVDVAC 126

Query: 149 IDLYYQHRIDLSVPIEETMGELKKLVEEGKIKYIGLSEASADMIRRAHAVHPITALQMEY 208
           IDLYYQHRID  VPIE T+GELKKLVEEGKIKYIGLSEASA  IRRAHAVHPITALQ+E+
Sbjct: 127 IDLYYQHRIDTRVPIEITIGELKKLVEEGKIKYIGLSEASASTIRRAHAVHPITALQIEW 186

Query: 209 SLWCRETEDEIIPLCRELGIGIVAYSPLGRGFF-GGKAVIESLPSESLLVKHPRFSKDSL 268
           SLW R+ E++IIP CRELGIGIVAYSPLGRGFF  G  ++E+L +  +    PRF +++L
Sbjct: 187 SLWSRDVEEDIIPTCRELGIGIVAYSPLGRGFFASGPKLVENLDNNDVRKTLPRFQQENL 246

Query: 269 KVNETIYERLVDLAAKHRCTTAQLALAWLLHQGIDIVPVPGTTKVGNLDSNIGSLSVKLT 328
             N+ ++E++  ++ K  CT AQLALAW+ HQG D+ P+PGTTK+ NL+ NIG+LSVKLT
Sbjct: 247 DHNKILFEKVSAMSEKKGCTPAQLALAWVHHQGDDVCPIPGTTKIENLNQNIGALSVKLT 306

Query: 329 EKDLKEISDAVPIDEVRGEREYDAFSSY 355
            +++ E+        V+GER     +++
Sbjct: 307 PEEMSELESLAQPGFVKGERSISILTTF 331

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038906695.13.4e-18994.24LOW QUALITY PROTEIN: perakine reductase-like [Benincasa hispida][more]
XP_022138494.15.8e-17387.13perakine reductase-like isoform X5 [Momordica charantia][more]
XP_022138493.12.7e-17084.18perakine reductase-like isoform X4 [Momordica charantia][more]
XP_022138492.14.6e-17083.71perakine reductase-like isoform X3 [Momordica charantia][more]
XP_022138490.12.1e-16780.98perakine reductase-like isoform X1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
C6TBN24.8e-13065.09Probable aldo-keto reductase 1 OS=Glycine max OX=3847 GN=AKR1 PE=2 SV=1[more]
Q3L1813.5e-12865.77Perakine reductase OS=Rauvolfia serpentina OX=4060 GN=PR PE=1 SV=1[more]
A2XRZ02.1e-10156.23Probable aldo-keto reductase 2 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_153... [more]
Q7XT993.6e-10156.23Probable aldo-keto reductase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g... [more]
F4HPY82.0e-9957.14Probable aldo-keto reductase 6 OS=Arabidopsis thaliana OX=3702 GN=At1g60750 PE=3... [more]
Match NameE-valueIdentityDescription
A0A6J1CA992.8e-17387.13perakine reductase-like isoform X5 OS=Momordica charantia OX=3673 GN=LOC11100965... [more]
A0A6J1CD501.3e-17084.18perakine reductase-like isoform X4 OS=Momordica charantia OX=3673 GN=LOC11100965... [more]
A0A6J1CB892.2e-17083.71perakine reductase-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC11100965... [more]
A0A6J1C9K61.0e-16780.98perakine reductase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC11100965... [more]
A0A6J1C9V63.1e-16480.16perakine reductase-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC11100965... [more]
Match NameE-valueIdentityDescription
AT1G60750.11.4e-10057.14NAD(P)-linked oxidoreductase superfamily protein [more]
AT1G60710.11.8e-10057.37NAD(P)-linked oxidoreductase superfamily protein [more]
AT1G60690.19.1e-10056.56NAD(P)-linked oxidoreductase superfamily protein [more]
AT1G60680.11.2e-9954.88NAD(P)-linked oxidoreductase superfamily protein [more]
AT1G60730.11.0e-9855.49NAD(P)-linked oxidoreductase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023210NADP-dependent oxidoreductase domainPFAMPF00248Aldo_ket_redcoord: 44..334
e-value: 1.1E-71
score: 241.4
IPR036812NADP-dependent oxidoreductase domain superfamilyGENE3D3.20.20.100coord: 29..365
e-value: 1.6E-111
score: 374.4
IPR036812NADP-dependent oxidoreductase domain superfamilySUPERFAMILY51430NAD(P)-linked oxidoreductasecoord: 31..336
NoneNo IPR availablePANTHERPTHR43625:SF65ALDO-KETO REDUCTASE 4-RELATEDcoord: 19..364
NoneNo IPR availablePANTHERPTHR43625AFLATOXIN B1 ALDEHYDE REDUCTASEcoord: 19..364
NoneNo IPR availableCDDcd19145AKR_AKR13D1coord: 30..333
e-value: 0.0
score: 520.067

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10010216.1HG10010216.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0047834 D-threo-aldose 1-dehydrogenase activity