HG10010134 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10010134
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionprotein NRT1/ PTR FAMILY 5.2-like
LocationChr06: 18810594 .. 18813580 (-)
RNA-Seq ExpressionHG10010134
SyntenyHG10010134
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGTCTCACTCCCAACCCTAAGGCCACCTCCGTGCGCGGAACCGACCTCCGGTCATTGCGAGAAGGCTTCCACACTGCAACTTGCCGTCTACTTTGGCGCTCTCTACACCCTCGCACTCGGCACGGGTGGAACAAAGCCGAATATATCGACCATCGGTGCAGACCAATTTGACGACTTCCACCCGAAGGAGAAGAAGCAAAAGCTCTCCTTTTTCAATTGGTGGATGTTCAGCATTTTCTTTGGCACTCTTTTTGCTAACATAATTCTTGTTTACATTCAAGACAACATTGGTTGGACCCTTGGTTATGGCCTTCCCACAATTGGCCTCGCCATTTCTATTGGTATTTTTGTCTCCGGAATTCCGTTCTACCGCCATAAGGTTCCAGCGGGAAGTCCGTTTACTCGAATGGCTAAGGTTATCGTCGCCGCCGTGAGGAAGCGGAAACTGCCGCTGCCCCAGGAGGCTAAGGAATTGTATGAGTTGGATTTGGAGGAGTATACGAAGAAGAGGAAGTTTAGGATGGAGGCCACTCCCTCTTTAAGGTATGTTTGACAAATTTATTTCAAACGTTTTTTTTATTTGAAAAAAAATAAATTTAAAGTTAAATTATAGGAAAATTTTTCCAAATAGAAAAAATATCAAACTATTTACAAAAAATAGTAAAAAAAAATACTGATAGACATTGATAGACTTCTATTAGCATCTATTAGTGATAGACTTCTATCGTTTCTATCACTGATAGACCCTCATAGATTTCTATTAAGGTCTATCACAACTATCTAAAAATTTTGCTATTTTGTATAAATAGTTTCCCTTATTTTTCTATTTTTAAAATTTACCTAAATTATAAATTTGATTTTGTTTAGTTTTAATACTTTGCCTTTTTTTAAAGAAATTTCTAATAGATCCTTGAACCTCGGTTATAGTTAGTCTTCATACTTTAAAAAAAAATCTACTAAGTCCATGATTTTGTTTTTGATAGGCATGGTGTTGGGCTAATGGTATATTTTTTAAACATTAGACTAAATGCAATTTTGGAGTCATGATCAATTTTATATCTTATCAACTCTCTACAATTGTGGATCCGATTAGTTTACAACTCCTTAAACTTTATAACTCCGTAGTCTTCACTATTCAATTCTTTACCCCAAGCACCCGTGAAAAGAAAATATTCTCCATTTGACCTTAGCTAACATGAATATAGCTCAATTAACATGAACTTGTATTATCAACTTCGAGGTCTGATGTTCGATCTTCACGCCACATATTGTAAAAGAAAAATGACCTTAGGACACGCTAGAGTTGAATCTTTGTTGAAATTTGTTAATTAAAGTTTTGAATGAGTTCTAATTGAAAGTTATTGTCTTCTCCAACATCAGCTTCCTGAACAAAGCTTCAATAAAAACTGGTTCAACTGATCCATGGCGGCTATGCACAGTAACAGAAGTGGAAGAAACAAAGCAAATGATCAGAATGATTCCAATTCTGTTTGCAACCTTTGTCCCAAGCATCATGTTGGCTCAAGTCAACACTCTGTTCATAAAACAGGGCACCACTCTGGATCGAAAAATCGGCAACTTTAACATCCCGCCCGCTAGCTTGAGCGGTTTCGTCACCGCTTCATTGCTCATAAGCGTCGTACTTTACGATCGAGTCTTCGTCAAAATCATGAGAAAATTCACCAAAAATCCAAGGGGAATCACTCTGCTTCAGCGGATGGGAATTGGGATTGTCCTCCATATGTTAATCATGGTCGTCTGTTCTCTCGTTGAGCGGCGCCGCTTGACCGTCGCCAGAGAGCACGGCGTTGTTCAGAGTGGCGGCCAAGTTCCATTGAGTATCTTCATTTTGCTGCCACAGTTTATTCTCATGGGGACTGCCGATGCCTTCATGGAGGTCTCCAAAATTGAATTCTTTTATGATCAGGTACTATCTCGTTTGCTAACAATTATGTTTGTAATTTTAAAAATTTTATTTATACGTCGACTACATCAACTTATTTTTGGAAAAATTAACTTAAAGTTTGAATATATACCCACTAAAAGAAGTGAAATTAGTATCTATAAGTTTAATTTTAAGAACATAACATTAAATTTATACTTTCTATGTTAAATGTAGTCTAATTTATGCTAGTTGAGCTTACCACCGGTCACTTTATGAATTGATTTAATTTTTGAGAAGAAATTATACTACATTAGATAAGAAAATTTCCTAATTTTTCTTCTAAATTTCTTTCTAAGAAGTTGCCTAAGTAAATCACTACGATTCGGGTAGTTAAGGTTACCACCAATTACATTTAACATCTGACAACATAAACAAGACAATTAGAAGTGCCATCAATATTAAAAAGACAAAAGAAAAGAGTTTCTCACATGTCAAACTTTGGTGGAACTAAAAACAAGTTCAACCTATGATGCATGCAAGTATTGAACACATCTCGGGTGACACTTATCTTTGTACAATAATTTATCTCATGACAAATTCTTCTTACTATTTTATTAAATATTTTAATTATTTTGTTGTGTGTGTGATGAGAATGAATGCATAAAGATAGGTATATCTTAGGATGCACCCAATATTGCTCTTTTTTCAAGTTAAAAAGTGTAAATAGATTATTAAAAAATCTAATCATTTATTTATATTCTTAACACTAGGCGCCAGAGAGCATGAAAAGTCTCGGGACTTCTTACTCGACGACAGCGATCGGGACAGGGAATTTCCTAAGTAGCTTCCTCCTCTCGACAGTTTCCGACCTTACCAGACGGCACGGGCGGAAGGGATGGATCTTGAACAACCTAAACGCATCTCATCTTGACTACTACTATGGCTTTTTCGCCATTCTCAACTTTTTAAACTTCATCTTCTTCTTGGTGGTTTCAAGGTATTATGTATACAAAGCTGAAGTTTCGGACTCCATAAGATTGCTTACTGAAGAACTGAAGGAGAAGGCACCATCTAAAGAATCAAACAACCCACATTGA

mRNA sequence

ATGGCAGTCTCACTCCCAACCCTAAGGCCACCTCCGTGCGCGGAACCGACCTCCGGTCATTGCGAGAAGGCTTCCACACTGCAACTTGCCGTCTACTTTGGCGCTCTCTACACCCTCGCACTCGGCACGGGTGGAACAAAGCCGAATATATCGACCATCGGTGCAGACCAATTTGACGACTTCCACCCGAAGGAGAAGAAGCAAAAGCTCTCCTTTTTCAATTGGTGGATGTTCAGCATTTTCTTTGGCACTCTTTTTGCTAACATAATTCTTGTTTACATTCAAGACAACATTGGTTGGACCCTTGGTTATGGCCTTCCCACAATTGGCCTCGCCATTTCTATTGGTATTTTTGTCTCCGGAATTCCGTTCTACCGCCATAAGGTTCCAGCGGGAAGTCCGTTTACTCGAATGGCTAAGGTTATCGTCGCCGCCGTGAGGAAGCGGAAACTGCCGCTGCCCCAGGAGGCTAAGGAATTGTATGAGTTGGATTTGGAGGAGTATACGAAGAAGAGGAAGTTTAGGATGGAGGCCACTCCCTCTTTAAGCTTCCTGAACAAAGCTTCAATAAAAACTGGTTCAACTGATCCATGGCGGCTATGCACAGTAACAGAAGTGGAAGAAACAAAGCAAATGATCAGAATGATTCCAATTCTGTTTGCAACCTTTGTCCCAAGCATCATGTTGGCTCAAGTCAACACTCTGTTCATAAAACAGGGCACCACTCTGGATCGAAAAATCGGCAACTTTAACATCCCGCCCGCTAGCTTGAGCGGTTTCGTCACCGCTTCATTGCTCATAAGCGTCGTACTTTACGATCGAGTCTTCGTCAAAATCATGAGAAAATTCACCAAAAATCCAAGGGGAATCACTCTGCTTCAGCGGATGGGAATTGGGATTGTCCTCCATATGTTAATCATGGTCGTCTGTTCTCTCGTTGAGCGGCGCCGCTTGACCGTCGCCAGAGAGCACGGCGTTGTTCAGAGTGGCGGCCAAGTTCCATTGAGTATCTTCATTTTGCTGCCACAGTTTATTCTCATGGGGACTGCCGATGCCTTCATGGAGGTCTCCAAAATTGAATTCTTTTATGATCAGGCGCCAGAGAGCATGAAAAGTCTCGGGACTTCTTACTCGACGACAGCGATCGGGACAGGGAATTTCCTAAGTAGCTTCCTCCTCTCGACAGTTTCCGACCTTACCAGACGGCACGGGCGGAAGGGATGGATCTTGAACAACCTAAACGCATCTCATCTTGACTACTACTATGGCTTTTTCGCCATTCTCAACTTTTTAAACTTCATCTTCTTCTTGGTGGTTTCAAGGTATTATGTATACAAAGCTGAAGTTTCGGACTCCATAAGATTGCTTACTGAAGAACTGAAGGAGAAGGCACCATCTAAAGAATCAAACAACCCACATTGA

Coding sequence (CDS)

ATGGCAGTCTCACTCCCAACCCTAAGGCCACCTCCGTGCGCGGAACCGACCTCCGGTCATTGCGAGAAGGCTTCCACACTGCAACTTGCCGTCTACTTTGGCGCTCTCTACACCCTCGCACTCGGCACGGGTGGAACAAAGCCGAATATATCGACCATCGGTGCAGACCAATTTGACGACTTCCACCCGAAGGAGAAGAAGCAAAAGCTCTCCTTTTTCAATTGGTGGATGTTCAGCATTTTCTTTGGCACTCTTTTTGCTAACATAATTCTTGTTTACATTCAAGACAACATTGGTTGGACCCTTGGTTATGGCCTTCCCACAATTGGCCTCGCCATTTCTATTGGTATTTTTGTCTCCGGAATTCCGTTCTACCGCCATAAGGTTCCAGCGGGAAGTCCGTTTACTCGAATGGCTAAGGTTATCGTCGCCGCCGTGAGGAAGCGGAAACTGCCGCTGCCCCAGGAGGCTAAGGAATTGTATGAGTTGGATTTGGAGGAGTATACGAAGAAGAGGAAGTTTAGGATGGAGGCCACTCCCTCTTTAAGCTTCCTGAACAAAGCTTCAATAAAAACTGGTTCAACTGATCCATGGCGGCTATGCACAGTAACAGAAGTGGAAGAAACAAAGCAAATGATCAGAATGATTCCAATTCTGTTTGCAACCTTTGTCCCAAGCATCATGTTGGCTCAAGTCAACACTCTGTTCATAAAACAGGGCACCACTCTGGATCGAAAAATCGGCAACTTTAACATCCCGCCCGCTAGCTTGAGCGGTTTCGTCACCGCTTCATTGCTCATAAGCGTCGTACTTTACGATCGAGTCTTCGTCAAAATCATGAGAAAATTCACCAAAAATCCAAGGGGAATCACTCTGCTTCAGCGGATGGGAATTGGGATTGTCCTCCATATGTTAATCATGGTCGTCTGTTCTCTCGTTGAGCGGCGCCGCTTGACCGTCGCCAGAGAGCACGGCGTTGTTCAGAGTGGCGGCCAAGTTCCATTGAGTATCTTCATTTTGCTGCCACAGTTTATTCTCATGGGGACTGCCGATGCCTTCATGGAGGTCTCCAAAATTGAATTCTTTTATGATCAGGCGCCAGAGAGCATGAAAAGTCTCGGGACTTCTTACTCGACGACAGCGATCGGGACAGGGAATTTCCTAAGTAGCTTCCTCCTCTCGACAGTTTCCGACCTTACCAGACGGCACGGGCGGAAGGGATGGATCTTGAACAACCTAAACGCATCTCATCTTGACTACTACTATGGCTTTTTCGCCATTCTCAACTTTTTAAACTTCATCTTCTTCTTGGTGGTTTCAAGGTATTATGTATACAAAGCTGAAGTTTCGGACTCCATAAGATTGCTTACTGAAGAACTGAAGGAGAAGGCACCATCTAAAGAATCAAACAACCCACATTGA

Protein sequence

MAVSLPTLRPPPCAEPTSGHCEKASTLQLAVYFGALYTLALGTGGTKPNISTIGADQFDDFHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAISIGIFVSGIPFYRHKVPAGSPFTRMAKVIVAAVRKRKLPLPQEAKELYELDLEEYTKKRKFRMEATPSLSFLNKASIKTGSTDPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQGTTLDRKIGNFNIPPASLSGFVTASLLISVVLYDRVFVKIMRKFTKNPRGITLLQRMGIGIVLHMLIMVVCSLVERRRLTVAREHGVVQSGGQVPLSIFILLPQFILMGTADAFMEVSKIEFFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVSDLTRRHGRKGWILNNLNASHLDYYYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEELKEKAPSKESNNPH
Homology
BLAST of HG10010134 vs. NCBI nr
Match: XP_038874759.1 (protein NRT1/ PTR FAMILY 5.2-like [Benincasa hispida])

HSP 1 Score: 895.6 bits (2313), Expect = 1.9e-256
Identity = 456/473 (96.41%), Postives = 465/473 (98.31%), Query Frame = 0

Query: 1   MAVSLPTLRPPPCAEPTSGHCEKASTLQLAVYFGALYTLALGTGGTKPNISTIGADQFDD 60
           MAVSLPTLRPPPCA+PTSGHCE+ASTLQLAVYFGALY LALGTGGTKPNISTIGADQFDD
Sbjct: 119 MAVSLPTLRPPPCAQPTSGHCERASTLQLAVYFGALYILALGTGGTKPNISTIGADQFDD 178

Query: 61  FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAISIGIFVS 120
           FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPT+GLA+SIGIFV+
Sbjct: 179 FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTLGLAVSIGIFVA 238

Query: 121 GIPFYRHKVPAGSPFTRMAKVIVAAVRKRKLPLPQEAKELYELDLEEYTKKRKFRMEATP 180
           GIPFYRHKVPAGSPFTRMAKV+VAAV+KRKLPLP+EAKELYELDLEEYTKKRKFRMEATP
Sbjct: 239 GIPFYRHKVPAGSPFTRMAKVLVAAVKKRKLPLPREAKELYELDLEEYTKKRKFRMEATP 298

Query: 181 SLSFLNKASIKTGSTDPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG 240
           SLSFLNKAS+KTGST PWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG
Sbjct: 299 SLSFLNKASVKTGSTHPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG 358

Query: 241 TTLDRKIGNFNIPPASLSGFVTASLLISVVLYDRVFVKIMRKFTKNPRGITLLQRMGIGI 300
           TTLDRKIG FNIPPASLSGFVT SLLISVVLYDRVFVKIMRKFTKNPRGITLLQRMGIGI
Sbjct: 359 TTLDRKIGKFNIPPASLSGFVTVSLLISVVLYDRVFVKIMRKFTKNPRGITLLQRMGIGI 418

Query: 301 VLHMLIMVVCSLVERRRLTVAREHGVVQSGGQVPLSIFILLPQFILMGTADAFMEVSKIE 360
           VLHMLIMVVCSLVER RL VAREHGVVQSGGQVPLSIFILLPQFILMGTADAFMEVSKIE
Sbjct: 419 VLHMLIMVVCSLVERHRLAVAREHGVVQSGGQVPLSIFILLPQFILMGTADAFMEVSKIE 478

Query: 361 FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVSDLTRRHGRKGWILNNLNASHLDY 420
           FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVSDLT RHGRKGWILNNLNASHLDY
Sbjct: 479 FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVSDLTSRHGRKGWILNNLNASHLDY 538

Query: 421 YYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEELKEKAPSKESNNPH 474
           YYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEELKEK PSKESNNPH
Sbjct: 539 YYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEELKEKTPSKESNNPH 591

BLAST of HG10010134 vs. NCBI nr
Match: XP_008460668.1 (PREDICTED: protein NRT1/ PTR FAMILY 5.2-like [Cucumis melo] >KAA0050720.1 protein NRT1/ PTR FAMILY 5.2-like [Cucumis melo var. makuwa] >TYK01452.1 protein NRT1/ PTR FAMILY 5.2-like [Cucumis melo var. makuwa])

HSP 1 Score: 883.2 bits (2281), Expect = 9.6e-253
Identity = 449/473 (94.93%), Postives = 463/473 (97.89%), Query Frame = 0

Query: 1   MAVSLPTLRPPPCAEPTSGHCEKASTLQLAVYFGALYTLALGTGGTKPNISTIGADQFDD 60
           MAVSLPTLRPPPCAEPTSGHCEKAS+LQLAVYFGALY LALGTGGTKPNISTIGADQFDD
Sbjct: 119 MAVSLPTLRPPPCAEPTSGHCEKASSLQLAVYFGALYILALGTGGTKPNISTIGADQFDD 178

Query: 61  FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAISIGIFVS 120
           FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPT+GLAISIGIFV+
Sbjct: 179 FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTLGLAISIGIFVA 238

Query: 121 GIPFYRHKVPAGSPFTRMAKVIVAAVRKRKLPLPQEAKELYELDLEEYTKKRKFRMEATP 180
           GIPFYRHKVPAGSPF+RM KV+VAA RKRKLPLP++ +ELYELDLEEYTKKRKFRMEATP
Sbjct: 239 GIPFYRHKVPAGSPFSRMGKVLVAAARKRKLPLPRDVEELYELDLEEYTKKRKFRMEATP 298

Query: 181 SLSFLNKASIKTGSTDPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG 240
           SLSFLNKASIKTGST PWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG
Sbjct: 299 SLSFLNKASIKTGSTHPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG 358

Query: 241 TTLDRKIGNFNIPPASLSGFVTASLLISVVLYDRVFVKIMRKFTKNPRGITLLQRMGIGI 300
           TTLDRKIGNF IPPASLSGFVT SLL+S+ LYDRVFVKIMRKFTKNPRGITLLQRMGIGI
Sbjct: 359 TTLDRKIGNFEIPPASLSGFVTVSLLVSIGLYDRVFVKIMRKFTKNPRGITLLQRMGIGI 418

Query: 301 VLHMLIMVVCSLVERRRLTVAREHGVVQSGGQVPLSIFILLPQFILMGTADAFMEVSKIE 360
           VLHMLIMVVCSLVERRRL VA+EHGVVQSG QVPLSIFILLPQFILMGTADAFMEVSKIE
Sbjct: 419 VLHMLIMVVCSLVERRRLAVAKEHGVVQSGAQVPLSIFILLPQFILMGTADAFMEVSKIE 478

Query: 361 FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVSDLTRRHGRKGWILNNLNASHLDY 420
           FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTV+DLT+R+GRKGWILNNLNASHLDY
Sbjct: 479 FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVADLTKRNGRKGWILNNLNASHLDY 538

Query: 421 YYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEELKEKAPSKESNNPH 474
           YYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEELKEKAPSKESNNPH
Sbjct: 539 YYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEELKEKAPSKESNNPH 591

BLAST of HG10010134 vs. NCBI nr
Match: XP_004147160.1 (protein NRT1/ PTR FAMILY 5.2 [Cucumis sativus] >KGN61581.1 hypothetical protein Csa_006774 [Cucumis sativus])

HSP 1 Score: 877.1 bits (2265), Expect = 6.9e-251
Identity = 448/474 (94.51%), Postives = 464/474 (97.89%), Query Frame = 0

Query: 1   MAVSLPTLRPPPCAEPTSGHCEKASTLQLAVYFGALYTLALGTGGTKPNISTIGADQFDD 60
           MAVSLPTLRPPPCA+PTSGHCEKAS+LQLAVYFGALY LA+GTGGTKPNISTIGADQFDD
Sbjct: 119 MAVSLPTLRPPPCADPTSGHCEKASSLQLAVYFGALYILAIGTGGTKPNISTIGADQFDD 178

Query: 61  FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAISIGIFVS 120
           FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPT+GLAISIGIFV+
Sbjct: 179 FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTLGLAISIGIFVA 238

Query: 121 GIPFYRHKVPAGSPFTRMAKVIVAAVRKRKLPLPQEAKELYELDLEEYTKKRKFRMEATP 180
           GIPFYRHKVPAGSPF+RM KV+VAA+RKRKLPLP++ KELYELDLEEYTKKRKFRMEATP
Sbjct: 239 GIPFYRHKVPAGSPFSRMGKVLVAAIRKRKLPLPRDVKELYELDLEEYTKKRKFRMEATP 298

Query: 181 SLSFLNKASIKTGSTDPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG 240
           SLSFLNKASIKTGST PWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG
Sbjct: 299 SLSFLNKASIKTGSTHPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG 358

Query: 241 TTLDRKIGNFNIPPASLSGFVTASLLISVVLYDRVFVKIMRKFTKNPRGITLLQRMGIGI 300
           TTL+RKIGNF IPPASLSGFVT SLLIS+ LYDRVFVKIMRKFTKNPRGITLLQRMGIGI
Sbjct: 359 TTLNRKIGNFEIPPASLSGFVTVSLLISIGLYDRVFVKIMRKFTKNPRGITLLQRMGIGI 418

Query: 301 VLHMLIMVVCSLVERRRLTVAREHGVVQSGGQVPLSIFILLPQFILMGTADAFMEVSKIE 360
           VLHMLIMVVCSLVERRRL VA+EHGVVQSG QVPLSIFILLPQFILMGTADAFMEVSKIE
Sbjct: 419 VLHMLIMVVCSLVERRRLAVAKEHGVVQSGAQVPLSIFILLPQFILMGTADAFMEVSKIE 478

Query: 361 FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVSDLTRRHGRKGWILNNLNASHLDY 420
           FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTV++LTRR+GRKGWILNNLNASHLDY
Sbjct: 479 FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVANLTRRNGRKGWILNNLNASHLDY 538

Query: 421 YYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEELKEKAPSKE-SNNPH 474
           YYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEELKEKAPSKE SNNPH
Sbjct: 539 YYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEELKEKAPSKESSNNPH 592

BLAST of HG10010134 vs. NCBI nr
Match: XP_022138841.1 (protein NRT1/ PTR FAMILY 5.2-like [Momordica charantia])

HSP 1 Score: 841.3 bits (2172), Expect = 4.2e-240
Identity = 431/470 (91.70%), Postives = 450/470 (95.74%), Query Frame = 0

Query: 1   MAVSLPTLRPPPCAEPTSGHCEKASTLQLAVYFGALYTLALGTGGTKPNISTIGADQFDD 60
           MAVSLPTLRPPPCAEPT+G+C KASTLQLAV++GALYTLA+GTGGTKPNISTIGADQFD+
Sbjct: 120 MAVSLPTLRPPPCAEPTTGNCGKASTLQLAVFYGALYTLAVGTGGTKPNISTIGADQFDE 179

Query: 61  FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAISIGIFVS 120
           F PKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAISIGIFV 
Sbjct: 180 FDPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAISIGIFVV 239

Query: 121 GIPFYRHKVPAGSPFTRMAKVIVAAVRKRKLPLPQEAKELYELDLEEYTKKRKFRMEATP 180
           GIPFYRHKVPAGSPFTRMAKVIVAA+R RKLPLP EAKELYELD+EEYTKK+KFRME TP
Sbjct: 240 GIPFYRHKVPAGSPFTRMAKVIVAAIRNRKLPLPVEAKELYELDVEEYTKKKKFRMEFTP 299

Query: 181 SLSFLNKASIKTGSTDPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG 240
           +LS LNKASI+TGST PWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG
Sbjct: 300 TLSILNKASIQTGSTHPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG 359

Query: 241 TTLDRKIGNFNIPPASLSGFVTASLLISVVLYDRVFVKIMRKFTKNPRGITLLQRMGIGI 300
           TTLDR+IGNF+IPPASLSG VT SLL+SVV+YDRVFV+IMRKFTKNPRGITLLQRMGIGI
Sbjct: 360 TTLDRRIGNFSIPPASLSGVVTLSLLVSVVVYDRVFVRIMRKFTKNPRGITLLQRMGIGI 419

Query: 301 VLHMLIMVVCSLVERRRLTVAREHGVVQSGGQVPLSIFILLPQFILMGTADAFMEVSKIE 360
           VLH LIM V SLVERRRL  AREHGVV SG QVPLSIFILLPQFILMGTADAFMEVSKIE
Sbjct: 420 VLHTLIMTVSSLVERRRLAAAREHGVVDSGAQVPLSIFILLPQFILMGTADAFMEVSKIE 479

Query: 361 FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVSDLTRRHGRKGWILNNLNASHLDY 420
           FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLL+TVSDLT RHGRKGWILNNLNASHLDY
Sbjct: 480 FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLATVSDLTSRHGRKGWILNNLNASHLDY 539

Query: 421 YYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEELKEKAPSKESN 471
           YYGFFAILN LNFIFFLVVSR+YVYKAEVSDSIRLLTEELK+K PSKESN
Sbjct: 540 YYGFFAILNCLNFIFFLVVSRFYVYKAEVSDSIRLLTEELKDKTPSKESN 589

BLAST of HG10010134 vs. NCBI nr
Match: XP_023515133.1 (protein NRT1/ PTR FAMILY 5.2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 836.6 bits (2160), Expect = 1.0e-238
Identity = 425/473 (89.85%), Postives = 448/473 (94.71%), Query Frame = 0

Query: 1   MAVSLPTLRPPPCAEPTSGHCEKASTLQLAVYFGALYTLALGTGGTKPNISTIGADQFDD 60
           MAVSLPTLRPP C +P SGHC+KASTLQLAVYFGALY LA+GTGGTKPNISTIGADQFDD
Sbjct: 119 MAVSLPTLRPPSCDQPNSGHCQKASTLQLAVYFGALYILAVGTGGTKPNISTIGADQFDD 178

Query: 61  FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAISIGIFVS 120
           FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLA+SIGIFV 
Sbjct: 179 FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAVSIGIFVV 238

Query: 121 GIPFYRHKVPAGSPFTRMAKVIVAAVRKRKLPLPQEAKELYELDLEEYTKKRKFRMEATP 180
           GIPFYRHKVPAGSPFTRMAKVIVAA+  RKLPLP++AKELYE + EEYTKK+KFRMEATP
Sbjct: 239 GIPFYRHKVPAGSPFTRMAKVIVAAINNRKLPLPRDAKELYEWNFEEYTKKKKFRMEATP 298

Query: 181 SLSFLNKASIKTGSTDPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG 240
           +LS LNKASI+TGSTDPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG
Sbjct: 299 TLSSLNKASIQTGSTDPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG 358

Query: 241 TTLDRKIGNFNIPPASLSGFVTASLLISVVLYDRVFVKIMRKFTKNPRGITLLQRMGIGI 300
           TTLDRK+GNF IPPASLS FVTA+LL+SVV+YDR FVKIMRKFTKNPRGITLLQRMG GI
Sbjct: 359 TTLDRKVGNFKIPPASLSAFVTATLLVSVVIYDRFFVKIMRKFTKNPRGITLLQRMGTGI 418

Query: 301 VLHMLIMVVCSLVERRRLTVAREHGVVQSGGQVPLSIFILLPQFILMGTADAFMEVSKIE 360
           VLH LIMVV  LVERRRL  AREHGVVQSG +VPLSIFILLPQFILMG ADAFMEVSKIE
Sbjct: 419 VLHTLIMVVSCLVERRRLAAAREHGVVQSGSEVPLSIFILLPQFILMGVADAFMEVSKIE 478

Query: 361 FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVSDLTRRHGRKGWILNNLNASHLDY 420
           FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLL+TVSD+T R+GRKGWILNNLNASHLDY
Sbjct: 479 FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLATVSDITSRNGRKGWILNNLNASHLDY 538

Query: 421 YYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEELKEKAPSKESNNPH 474
           YY FFAILN LNFIFFL VSRYYVYKAEVSDSI+LL+E+LKEKAPSKESNNP+
Sbjct: 539 YYAFFAILNCLNFIFFLFVSRYYVYKAEVSDSIKLLSEQLKEKAPSKESNNPY 591

BLAST of HG10010134 vs. ExPASy Swiss-Prot
Match: Q9FNL7 (Protein NRT1/ PTR FAMILY 5.2 OS=Arabidopsis thaliana OX=3702 GN=NPF5.2 PE=2 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 5.2e-185
Identity = 321/464 (69.18%), Postives = 394/464 (84.91%), Query Frame = 0

Query: 1   MAVSLPTLRPPPCAEPTSGHCEKASTLQLAVYFGALYTLALGTGGTKPNISTIGADQFDD 60
           ++V++P ++PP C+     +CEKAS LQLAV+FGALYTLA+GTGGTKPNISTIGADQFD 
Sbjct: 118 LSVTIPGIKPPECSTTNVENCEKASVLQLAVFFGALYTLAIGTGGTKPNISTIGADQFDV 177

Query: 61  FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAISIGIFVS 120
           F PKEK QKLSFFNWWMFSIFFGTLFAN +LVY+QDN+GWTLGYGLPT+GLAISI IF+ 
Sbjct: 178 FDPKEKTQKLSFFNWWMFSIFFGTLFANTVLVYVQDNVGWTLGYGLPTLGLAISITIFLL 237

Query: 121 GIPFYRHKVPAGSPFTRMAKVIVAAVRKRKLPLPQEAKELYELDLEEYTKKRKFRMEATP 180
           G PFYRHK+P GSPFT+MA+VIVA+ RK   P+  +    +EL   EY +K  F +  TP
Sbjct: 238 GTPFYRHKLPTGSPFTKMARVIVASFRKANAPMTHDITSFHELPSLEYERKGAFPIHPTP 297

Query: 181 SLSFLNKASIKTGSTDPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG 240
           SL FL++AS+KTG+   W LCT TEVEETKQM+RM+P+LF TFVPS+MLAQ+NTLF+KQG
Sbjct: 298 SLRFLDRASLKTGTNHKWNLCTTTEVEETKQMLRMLPVLFITFVPSMMLAQINTLFVKQG 357

Query: 241 TTLDRKI-GNFNIPPASLSGFVTASLLISVVLYDRVFVKIMRKFTKNPRGITLLQRMGIG 300
           TTLDRK+ G+F+IPPASLSGFVT S+LIS+VLYDRVFVKI RKFT NPRGITLLQRMGIG
Sbjct: 358 TTLDRKVTGSFSIPPASLSGFVTLSMLISIVLYDRVFVKITRKFTGNPRGITLLQRMGIG 417

Query: 301 IVLHMLIMVVCSLVERRRLTVAREHGVV-QSGGQVPLSIFILLPQFILMGTADAFMEVSK 360
           ++ H+LIM+V S+ ER RL VA +HG++ Q+G ++PL+IF LLPQF+LMG AD+F+EV+K
Sbjct: 418 LIFHILIMIVASVTERYRLKVAADHGLIHQTGVKLPLTIFALLPQFVLMGMADSFLEVAK 477

Query: 361 IEFFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVSDLTRRHGRKGWILNNLNASHL 420
           +EFFYDQAPESMKSLGTSYSTT++  GNF+SSFLLSTVS++T++ GR GWILNNLN S L
Sbjct: 478 LEFFYDQAPESMKSLGTSYSTTSLAIGNFMSSFLLSTVSEITKKRGR-GWILNNLNESRL 537

Query: 421 DYYYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEELKE 463
           DYYY FFA+LN +NF+ FLVV ++YVY+AEV+DS+ +   E+KE
Sbjct: 538 DYYYLFFAVLNLVNFVLFLVVVKFYVYRAEVTDSVDVKEVEMKE 580

BLAST of HG10010134 vs. ExPASy Swiss-Prot
Match: Q9FNL8 (Protein NRT1/ PTR FAMILY 5.3 OS=Arabidopsis thaliana OX=3702 GN=NPF5.3 PE=2 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 1.9e-171
Identity = 301/461 (65.29%), Postives = 375/461 (81.34%), Query Frame = 0

Query: 1   MAVSLPTLRPPPCAEPTSGHCEKASTLQLAVYFGALYTLALGTGGTKPNISTIGADQFDD 60
           ++VSLP L+PP C+     +CEKAS +QLAV+FGALYTLA+GTGGTKPNISTIGADQFD+
Sbjct: 118 LSVSLPGLKPPKCSTANVENCEKASVIQLAVFFGALYTLAIGTGGTKPNISTIGADQFDE 177

Query: 61  FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAISIGIFVS 120
           F PK+K  K SFFNWWMFSIFFGT FA  +LVY+QDN+GW +GYGL T+GLA SI IF+ 
Sbjct: 178 FDPKDKIHKHSFFNWWMFSIFFGTFFATTVLVYVQDNVGWAIGYGLSTLGLAFSIFIFLL 237

Query: 121 GIPFYRHKVPAGSPFTRMAKVIVAAVRKRKLPLPQEAKELYELDLEEYTKKRKFRMEATP 180
           G   YRHK+P GSPFT+MA+VIVA++RK + P+  ++   YEL   EY  KR F + +T 
Sbjct: 238 GTRLYRHKLPMGSPFTKMARVIVASLRKAREPMSSDSTRFYELPPMEYASKRAFPIHSTS 297

Query: 181 SLSFLNKASIKTGSTDPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG 240
           SL FLN+AS+KTGST  WRLCT+TEVEETKQM++M+P+LF TFVPS+MLAQ+ TLFIKQG
Sbjct: 298 SLRFLNRASLKTGSTHKWRLCTITEVEETKQMLKMLPVLFVTFVPSMMLAQIMTLFIKQG 357

Query: 241 TTLDRKI-GNFNIPPASLSGFVTASLLISVVLYDRVFVKIMRKFTKNPRGITLLQRMGIG 300
           TTLDR++  NF+IPPASL GF T S+L+S+V+YDRVFVK MRK T NPRGITLLQRMGIG
Sbjct: 358 TTLDRRLTNNFSIPPASLLGFTTFSMLVSIVIYDRVFVKFMRKLTGNPRGITLLQRMGIG 417

Query: 301 IVLHMLIMVVCSLVERRRLTVAREHGVV-QSGGQVPLSIFILLPQFILMGTADAFMEVSK 360
           ++LH+LIM++ S+ ER RL VA EHG+  Q+   +PLSIF LLPQ++LMG ADAF+E++K
Sbjct: 418 MILHILIMIIASITERYRLKVAAEHGLTHQTAVPIPLSIFTLLPQYVLMGLADAFIEIAK 477

Query: 361 IEFFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVSDLTRRHGRKGWILNNLNASHL 420
           +EFFYDQAPESMKSLGTSY++T++  G F+SS LLS+VS +T++ GR GWI NNLN S L
Sbjct: 478 LEFFYDQAPESMKSLGTSYTSTSMAVGYFMSSILLSSVSQITKKQGR-GWIQNNLNESRL 537

Query: 421 DYYYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEE 460
           D YY FFA+LN LNFI FLVV R+Y Y+A+V+ S  +  +E
Sbjct: 538 DNYYMFFAVLNLLNFILFLVVIRFYEYRADVTQSANVEQKE 577

BLAST of HG10010134 vs. ExPASy Swiss-Prot
Match: Q8VZR7 (Protein NRT1/ PTR FAMILY 5.1 OS=Arabidopsis thaliana OX=3702 GN=NPF5.1 PE=2 SV=2)

HSP 1 Score: 443.4 bits (1139), Expect = 3.3e-123
Identity = 234/450 (52.00%), Postives = 313/450 (69.56%), Query Frame = 0

Query: 1   MAVSLPTLRPPPCAEPTSGHCEKASTLQLAVYFGALYTLALGTGGTKPNISTIGADQFDD 60
           MAV++ +LR P C    +G C KAS+LQ+  ++ +LYT+A+G GGTKPNIST GADQFD 
Sbjct: 115 MAVTVKSLR-PTC---ENGVCNKASSLQVTFFYISLYTIAIGAGGTKPNISTFGADQFDS 174

Query: 61  FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAISIGIFVS 120
           +  +EKKQK+SFFNWWMFS F G LFA + LVYIQ+N+GW LGYG+PT+GL +S+ +F  
Sbjct: 175 YSIEEKKQKVSFFNWWMFSSFLGALFATLGLVYIQENLGWGLGYGIPTVGLLVSLVVFYI 234

Query: 121 GIPFYRHKVPAGSPFTR-MAKVIVAAVRKRKLPLPQEAKELYELDLEEYTKKRKFRMEAT 180
           G PFYRHKV       + + +V +AA + RKL  P +  ELYELD   Y    K ++  T
Sbjct: 235 GTPFYRHKVIKTDNLAKDLVQVPIAAFKNRKLQCPDDHLELYELDSHYYKSNGKHQVHHT 294

Query: 181 PSLSFLNKASIKTGSTDPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQ 240
           P   FL+KA+IKT S  P   CTVT+VE  K+++ +I I   T +PS + AQVNTLF+KQ
Sbjct: 295 PVFRFLDKAAIKTSSRVP---CTVTKVEVAKRVLGLIFIWLVTLIPSTLWAQVNTLFVKQ 354

Query: 241 GTTLDRKIG-NFNIPPASLSGFVTASLLISVVLYDRVFVKIMRKFTKNPRGITLLQRMGI 300
           GTTLDRKIG NF IP ASL  FVT S+L+SV +YD+ FV  MRK T NPRGITLLQR+G+
Sbjct: 355 GTTLDRKIGSNFQIPAASLGSFVTLSMLLSVPMYDQSFVPFMRKKTGNPRGITLLQRLGV 414

Query: 301 GIVLHMLIMVVCSLVERRRLTVAREHGVVQSGGQVPLSIFILLPQFILMGTADAFMEVSK 360
           G  + ++ + + S VE +R+ V +E  +      VP+SIF LLPQ+ L+G  D F  +  
Sbjct: 415 GFAIQIVAIAIASAVEVKRMRVIKEFHITSPTQVVPMSIFWLLPQYSLLGIGDVFNAIGL 474

Query: 361 IEFFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVSDLTRRHGRKGWILNNLNASHL 420
           +EFFYDQ+PE M+SLGT++ T+ IG GNFL+SFL++ +  +T + G K WI NNLN S L
Sbjct: 475 LEFFYDQSPEEMQSLGTTFFTSGIGLGNFLNSFLVTMIDKITSKGGGKSWIGNNLNDSRL 534

Query: 421 DYYYGFFAILNFLNFIFFLVVSRYYVYKAE 449
           DYYYGF  +++ +N   F+  +  YVYK++
Sbjct: 535 DYYYGFLVVISIVNMGLFVWAASKYVYKSD 557

BLAST of HG10010134 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 5.2e-100
Identity = 193/455 (42.42%), Postives = 300/455 (65.93%), Query Frame = 0

Query: 1   MAVSLPTLRPPPCAEPTSGHCEKASTLQLAVYFGALYTLALGTGGTKPNISTIGADQFDD 60
           ++ S+P L+P  C       C  A+  Q A++FG LY +ALGTGG KP +S+ GADQFDD
Sbjct: 132 LSASVPALKPAEC---IGDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDD 191

Query: 61  FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAISIGIFVS 120
              +E+ +K SFFNW+ FSI  G L ++ +LV+IQ+N GW LG+G+PT+ + ++I  F  
Sbjct: 192 TDSRERVRKASFFNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFF 251

Query: 121 GIPFYRHKVPAGSPFTRMAKVIVAAVRKRKLPLPQEAKELYELDLEEYTKKRKFRMEATP 180
           G P YR + P GSP TR+++V+VA+ RK  + +P++A  LYE   +        ++E T 
Sbjct: 252 GTPLYRFQKPGGSPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTD 311

Query: 181 SLSFLNKASI------KTGS-TDPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVN 240
              +L+KA++      K+G  ++ WRLCTVT+VEE K +IRM PI  +  + S + AQ++
Sbjct: 312 DCQYLDKAAVISEEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMS 371

Query: 241 TLFIKQGTTLDRKIGNFNIPPASLSGFVTASLLISVVLYDRVFVKIMRKFTKNPRGITLL 300
           T+F++QG  ++ KIG+F +PPA+L  F TAS++I V LYDR  V + RKFT   +G T +
Sbjct: 372 TMFVQQGRAMNCKIGSFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEI 431

Query: 301 QRMGIGIVLHMLIMVVCSLVERRRLTVAREHGVVQSGGQVPLSIFILLPQFILMGTADAF 360
           QRMGIG+ + +L M   ++VE  RL +A + G+V+SG  VP+S+   +PQ+ ++G A+ F
Sbjct: 432 QRMGIGLFVSVLCMAAAAIVEIIRLHMANDLGLVESGAPVPISVLWQIPQYFILGAAEVF 491

Query: 361 MEVSKIEFFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVSDLTRRHGRKGWILNNL 420
             + ++EFFYDQ+P++M+SL ++ +      GN+LSS +L+ V+  T R+G++GWI +NL
Sbjct: 492 YFIGQLEFFYDQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNL 551

Query: 421 NASHLDYYYGFFAILNFLNF-IFFLVVSRYYVYKA 448
           N+ HLDY++   A L+ +N  ++F   +RY   KA
Sbjct: 552 NSGHLDYFFWLLAGLSLVNMAVYFFSAARYKQKKA 583

BLAST of HG10010134 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 1.7e-98
Identity = 198/457 (43.33%), Postives = 295/457 (64.55%), Query Frame = 0

Query: 1   MAVSLPTLRPPPCAEPTSGHCEKASTLQLAVYFGALYTLALGTGGTKPNISTIGADQFDD 60
           ++ S+P L+P  C   T   C   S+ Q AV+F ALY +ALGTGG KP +S+ GADQFD+
Sbjct: 115 LSASVPGLKPGNCNADT---CHPNSS-QTAVFFVALYMIALGTGGIKPCVSSFGADQFDE 174

Query: 61  FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAISIGIFVS 120
               EK +K SFFNW+ FSI  G L A  +LV+IQ N+GW  G+G+PT+ + I++  F  
Sbjct: 175 NDENEKIKKSSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFF 234

Query: 121 GIPFYRHKVPAGSPFTRMAKVIVAAVRKRKLPLPQEAKELYELDLEEYTKKRKFRMEATP 180
           G  FYR + P GSP TR+ +VIVAA RK  + +P++   L+E   +E   K   ++  T 
Sbjct: 235 GSRFYRLQRPGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTD 294

Query: 181 SLSFLNKA-------SIKTGSTDPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVN 240
           +L F +KA       SIK G  +PWRLC+VT+VEE K +I ++P+     V + + +Q++
Sbjct: 295 NLKFFDKAAVESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMS 354

Query: 241 TLFIKQGTTLDRKIG-NFNIPPASLSGFVTASLLISVVLYDRVFVKIMRKFTKNPRGITL 300
           T+F+ QG T+D+ +G NF IP ASLS F T S+L    +YD+  + + RKFT+N RG T 
Sbjct: 355 TMFVLQGNTMDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQ 414

Query: 301 LQRMGIGIVLHMLIMVVCSLVERRRLTVAREHGVVQSGGQVPLSIFILLPQFILMGTADA 360
           LQRMGIG+V+ +  M+   ++E  RL   + H       Q+ +SIF  +PQ++L+G A+ 
Sbjct: 415 LQRMGIGLVVSIFAMITAGVLEVVRLDYVKTHNAYDQ-KQIHMSIFWQIPQYLLIGCAEV 474

Query: 361 FMEVSKIEFFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVSDLTRRHGRKGWILNN 420
           F  + ++EFFYDQAP++M+SL ++ S T +  GN+LS+ L++ V  +T+++G+ GWI +N
Sbjct: 475 FTFIGQLEFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDN 534

Query: 421 LNASHLDYYYGFFAILNFLNFIFFLVVSRYYVYKAEV 450
           LN  HLDY++   A L+FLNF+ +L +S+ Y YK  V
Sbjct: 535 LNRGHLDYFFYLLATLSFLNFLVYLWISKRYKYKKAV 566

BLAST of HG10010134 vs. ExPASy TrEMBL
Match: A0A5D3BRP9 (Protein NRT1/ PTR FAMILY 5.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold772G00450 PE=3 SV=1)

HSP 1 Score: 883.2 bits (2281), Expect = 4.6e-253
Identity = 449/473 (94.93%), Postives = 463/473 (97.89%), Query Frame = 0

Query: 1   MAVSLPTLRPPPCAEPTSGHCEKASTLQLAVYFGALYTLALGTGGTKPNISTIGADQFDD 60
           MAVSLPTLRPPPCAEPTSGHCEKAS+LQLAVYFGALY LALGTGGTKPNISTIGADQFDD
Sbjct: 119 MAVSLPTLRPPPCAEPTSGHCEKASSLQLAVYFGALYILALGTGGTKPNISTIGADQFDD 178

Query: 61  FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAISIGIFVS 120
           FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPT+GLAISIGIFV+
Sbjct: 179 FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTLGLAISIGIFVA 238

Query: 121 GIPFYRHKVPAGSPFTRMAKVIVAAVRKRKLPLPQEAKELYELDLEEYTKKRKFRMEATP 180
           GIPFYRHKVPAGSPF+RM KV+VAA RKRKLPLP++ +ELYELDLEEYTKKRKFRMEATP
Sbjct: 239 GIPFYRHKVPAGSPFSRMGKVLVAAARKRKLPLPRDVEELYELDLEEYTKKRKFRMEATP 298

Query: 181 SLSFLNKASIKTGSTDPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG 240
           SLSFLNKASIKTGST PWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG
Sbjct: 299 SLSFLNKASIKTGSTHPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG 358

Query: 241 TTLDRKIGNFNIPPASLSGFVTASLLISVVLYDRVFVKIMRKFTKNPRGITLLQRMGIGI 300
           TTLDRKIGNF IPPASLSGFVT SLL+S+ LYDRVFVKIMRKFTKNPRGITLLQRMGIGI
Sbjct: 359 TTLDRKIGNFEIPPASLSGFVTVSLLVSIGLYDRVFVKIMRKFTKNPRGITLLQRMGIGI 418

Query: 301 VLHMLIMVVCSLVERRRLTVAREHGVVQSGGQVPLSIFILLPQFILMGTADAFMEVSKIE 360
           VLHMLIMVVCSLVERRRL VA+EHGVVQSG QVPLSIFILLPQFILMGTADAFMEVSKIE
Sbjct: 419 VLHMLIMVVCSLVERRRLAVAKEHGVVQSGAQVPLSIFILLPQFILMGTADAFMEVSKIE 478

Query: 361 FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVSDLTRRHGRKGWILNNLNASHLDY 420
           FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTV+DLT+R+GRKGWILNNLNASHLDY
Sbjct: 479 FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVADLTKRNGRKGWILNNLNASHLDY 538

Query: 421 YYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEELKEKAPSKESNNPH 474
           YYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEELKEKAPSKESNNPH
Sbjct: 539 YYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEELKEKAPSKESNNPH 591

BLAST of HG10010134 vs. ExPASy TrEMBL
Match: A0A1S3CCI5 (protein NRT1/ PTR FAMILY 5.2-like OS=Cucumis melo OX=3656 GN=LOC103499437 PE=3 SV=1)

HSP 1 Score: 883.2 bits (2281), Expect = 4.6e-253
Identity = 449/473 (94.93%), Postives = 463/473 (97.89%), Query Frame = 0

Query: 1   MAVSLPTLRPPPCAEPTSGHCEKASTLQLAVYFGALYTLALGTGGTKPNISTIGADQFDD 60
           MAVSLPTLRPPPCAEPTSGHCEKAS+LQLAVYFGALY LALGTGGTKPNISTIGADQFDD
Sbjct: 119 MAVSLPTLRPPPCAEPTSGHCEKASSLQLAVYFGALYILALGTGGTKPNISTIGADQFDD 178

Query: 61  FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAISIGIFVS 120
           FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPT+GLAISIGIFV+
Sbjct: 179 FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTLGLAISIGIFVA 238

Query: 121 GIPFYRHKVPAGSPFTRMAKVIVAAVRKRKLPLPQEAKELYELDLEEYTKKRKFRMEATP 180
           GIPFYRHKVPAGSPF+RM KV+VAA RKRKLPLP++ +ELYELDLEEYTKKRKFRMEATP
Sbjct: 239 GIPFYRHKVPAGSPFSRMGKVLVAAARKRKLPLPRDVEELYELDLEEYTKKRKFRMEATP 298

Query: 181 SLSFLNKASIKTGSTDPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG 240
           SLSFLNKASIKTGST PWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG
Sbjct: 299 SLSFLNKASIKTGSTHPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG 358

Query: 241 TTLDRKIGNFNIPPASLSGFVTASLLISVVLYDRVFVKIMRKFTKNPRGITLLQRMGIGI 300
           TTLDRKIGNF IPPASLSGFVT SLL+S+ LYDRVFVKIMRKFTKNPRGITLLQRMGIGI
Sbjct: 359 TTLDRKIGNFEIPPASLSGFVTVSLLVSIGLYDRVFVKIMRKFTKNPRGITLLQRMGIGI 418

Query: 301 VLHMLIMVVCSLVERRRLTVAREHGVVQSGGQVPLSIFILLPQFILMGTADAFMEVSKIE 360
           VLHMLIMVVCSLVERRRL VA+EHGVVQSG QVPLSIFILLPQFILMGTADAFMEVSKIE
Sbjct: 419 VLHMLIMVVCSLVERRRLAVAKEHGVVQSGAQVPLSIFILLPQFILMGTADAFMEVSKIE 478

Query: 361 FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVSDLTRRHGRKGWILNNLNASHLDY 420
           FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTV+DLT+R+GRKGWILNNLNASHLDY
Sbjct: 479 FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVADLTKRNGRKGWILNNLNASHLDY 538

Query: 421 YYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEELKEKAPSKESNNPH 474
           YYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEELKEKAPSKESNNPH
Sbjct: 539 YYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEELKEKAPSKESNNPH 591

BLAST of HG10010134 vs. ExPASy TrEMBL
Match: A0A0A0LNM7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G172510 PE=3 SV=1)

HSP 1 Score: 877.1 bits (2265), Expect = 3.3e-251
Identity = 448/474 (94.51%), Postives = 464/474 (97.89%), Query Frame = 0

Query: 1   MAVSLPTLRPPPCAEPTSGHCEKASTLQLAVYFGALYTLALGTGGTKPNISTIGADQFDD 60
           MAVSLPTLRPPPCA+PTSGHCEKAS+LQLAVYFGALY LA+GTGGTKPNISTIGADQFDD
Sbjct: 119 MAVSLPTLRPPPCADPTSGHCEKASSLQLAVYFGALYILAIGTGGTKPNISTIGADQFDD 178

Query: 61  FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAISIGIFVS 120
           FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPT+GLAISIGIFV+
Sbjct: 179 FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTLGLAISIGIFVA 238

Query: 121 GIPFYRHKVPAGSPFTRMAKVIVAAVRKRKLPLPQEAKELYELDLEEYTKKRKFRMEATP 180
           GIPFYRHKVPAGSPF+RM KV+VAA+RKRKLPLP++ KELYELDLEEYTKKRKFRMEATP
Sbjct: 239 GIPFYRHKVPAGSPFSRMGKVLVAAIRKRKLPLPRDVKELYELDLEEYTKKRKFRMEATP 298

Query: 181 SLSFLNKASIKTGSTDPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG 240
           SLSFLNKASIKTGST PWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG
Sbjct: 299 SLSFLNKASIKTGSTHPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG 358

Query: 241 TTLDRKIGNFNIPPASLSGFVTASLLISVVLYDRVFVKIMRKFTKNPRGITLLQRMGIGI 300
           TTL+RKIGNF IPPASLSGFVT SLLIS+ LYDRVFVKIMRKFTKNPRGITLLQRMGIGI
Sbjct: 359 TTLNRKIGNFEIPPASLSGFVTVSLLISIGLYDRVFVKIMRKFTKNPRGITLLQRMGIGI 418

Query: 301 VLHMLIMVVCSLVERRRLTVAREHGVVQSGGQVPLSIFILLPQFILMGTADAFMEVSKIE 360
           VLHMLIMVVCSLVERRRL VA+EHGVVQSG QVPLSIFILLPQFILMGTADAFMEVSKIE
Sbjct: 419 VLHMLIMVVCSLVERRRLAVAKEHGVVQSGAQVPLSIFILLPQFILMGTADAFMEVSKIE 478

Query: 361 FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVSDLTRRHGRKGWILNNLNASHLDY 420
           FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTV++LTRR+GRKGWILNNLNASHLDY
Sbjct: 479 FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVANLTRRNGRKGWILNNLNASHLDY 538

Query: 421 YYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEELKEKAPSKE-SNNPH 474
           YYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEELKEKAPSKE SNNPH
Sbjct: 539 YYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEELKEKAPSKESSNNPH 592

BLAST of HG10010134 vs. ExPASy TrEMBL
Match: A0A6J1CAW6 (protein NRT1/ PTR FAMILY 5.2-like OS=Momordica charantia OX=3673 GN=LOC111009912 PE=3 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 2.0e-240
Identity = 431/470 (91.70%), Postives = 450/470 (95.74%), Query Frame = 0

Query: 1   MAVSLPTLRPPPCAEPTSGHCEKASTLQLAVYFGALYTLALGTGGTKPNISTIGADQFDD 60
           MAVSLPTLRPPPCAEPT+G+C KASTLQLAV++GALYTLA+GTGGTKPNISTIGADQFD+
Sbjct: 120 MAVSLPTLRPPPCAEPTTGNCGKASTLQLAVFYGALYTLAVGTGGTKPNISTIGADQFDE 179

Query: 61  FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAISIGIFVS 120
           F PKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAISIGIFV 
Sbjct: 180 FDPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAISIGIFVV 239

Query: 121 GIPFYRHKVPAGSPFTRMAKVIVAAVRKRKLPLPQEAKELYELDLEEYTKKRKFRMEATP 180
           GIPFYRHKVPAGSPFTRMAKVIVAA+R RKLPLP EAKELYELD+EEYTKK+KFRME TP
Sbjct: 240 GIPFYRHKVPAGSPFTRMAKVIVAAIRNRKLPLPVEAKELYELDVEEYTKKKKFRMEFTP 299

Query: 181 SLSFLNKASIKTGSTDPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG 240
           +LS LNKASI+TGST PWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG
Sbjct: 300 TLSILNKASIQTGSTHPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG 359

Query: 241 TTLDRKIGNFNIPPASLSGFVTASLLISVVLYDRVFVKIMRKFTKNPRGITLLQRMGIGI 300
           TTLDR+IGNF+IPPASLSG VT SLL+SVV+YDRVFV+IMRKFTKNPRGITLLQRMGIGI
Sbjct: 360 TTLDRRIGNFSIPPASLSGVVTLSLLVSVVVYDRVFVRIMRKFTKNPRGITLLQRMGIGI 419

Query: 301 VLHMLIMVVCSLVERRRLTVAREHGVVQSGGQVPLSIFILLPQFILMGTADAFMEVSKIE 360
           VLH LIM V SLVERRRL  AREHGVV SG QVPLSIFILLPQFILMGTADAFMEVSKIE
Sbjct: 420 VLHTLIMTVSSLVERRRLAAAREHGVVDSGAQVPLSIFILLPQFILMGTADAFMEVSKIE 479

Query: 361 FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVSDLTRRHGRKGWILNNLNASHLDY 420
           FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLL+TVSDLT RHGRKGWILNNLNASHLDY
Sbjct: 480 FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLATVSDLTSRHGRKGWILNNLNASHLDY 539

Query: 421 YYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEELKEKAPSKESN 471
           YYGFFAILN LNFIFFLVVSR+YVYKAEVSDSIRLLTEELK+K PSKESN
Sbjct: 540 YYGFFAILNCLNFIFFLVVSRFYVYKAEVSDSIRLLTEELKDKTPSKESN 589

BLAST of HG10010134 vs. ExPASy TrEMBL
Match: A0A6J1KMR2 (protein NRT1/ PTR FAMILY 5.2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111494736 PE=3 SV=1)

HSP 1 Score: 835.1 bits (2156), Expect = 1.5e-238
Identity = 425/473 (89.85%), Postives = 448/473 (94.71%), Query Frame = 0

Query: 1   MAVSLPTLRPPPCAEPTSGHCEKASTLQLAVYFGALYTLALGTGGTKPNISTIGADQFDD 60
           MAVSLPTLRPP C +P SGHC+KASTLQLAVYFGALY LA+GTGGTKPNISTIGADQFDD
Sbjct: 119 MAVSLPTLRPPSCDQPNSGHCQKASTLQLAVYFGALYILAVGTGGTKPNISTIGADQFDD 178

Query: 61  FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAISIGIFVS 120
           FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLA+SIGIFV 
Sbjct: 179 FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAVSIGIFVV 238

Query: 121 GIPFYRHKVPAGSPFTRMAKVIVAAVRKRKLPLPQEAKELYELDLEEYTKKRKFRMEATP 180
           GIPFYRHKVPAGSPFTRMAKVIVAA+  RKLPLP++AKELYE + EEYTKK+KFRMEATP
Sbjct: 239 GIPFYRHKVPAGSPFTRMAKVIVAAMNNRKLPLPRDAKELYEWNFEEYTKKKKFRMEATP 298

Query: 181 SLSFLNKASIKTGSTDPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG 240
           +LS LNKASI+TGSTDPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG
Sbjct: 299 TLSSLNKASIQTGSTDPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG 358

Query: 241 TTLDRKIGNFNIPPASLSGFVTASLLISVVLYDRVFVKIMRKFTKNPRGITLLQRMGIGI 300
           TTLDRK+GNF IPPASLS FVTA+LL+SVV+YDR FVKIMRKFTKNPRGITLLQRMG GI
Sbjct: 359 TTLDRKVGNFKIPPASLSAFVTATLLVSVVIYDRFFVKIMRKFTKNPRGITLLQRMGTGI 418

Query: 301 VLHMLIMVVCSLVERRRLTVAREHGVVQSGGQVPLSIFILLPQFILMGTADAFMEVSKIE 360
           VLH LIMVV  LVERRRL  AREHGVV+SG +VPLSIFILLPQFILMG ADAFMEVSKIE
Sbjct: 419 VLHTLIMVVSCLVERRRLAAAREHGVVESGREVPLSIFILLPQFILMGVADAFMEVSKIE 478

Query: 361 FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVSDLTRRHGRKGWILNNLNASHLDY 420
           FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLL+TVSD+T R+GRKGWILNNLNASHLDY
Sbjct: 479 FFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLATVSDITSRNGRKGWILNNLNASHLDY 538

Query: 421 YYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEELKEKAPSKESNNPH 474
           YY FFAILN LNFIFFL VSRYYVYKAEVSDSI+LL+EELKEKAPSKESNNP+
Sbjct: 539 YYAFFAILNCLNFIFFLFVSRYYVYKAEVSDSIKLLSEELKEKAPSKESNNPY 591

BLAST of HG10010134 vs. TAIR 10
Match: AT5G46050.1 (peptide transporter 3 )

HSP 1 Score: 648.7 bits (1672), Expect = 3.7e-186
Identity = 321/464 (69.18%), Postives = 394/464 (84.91%), Query Frame = 0

Query: 1   MAVSLPTLRPPPCAEPTSGHCEKASTLQLAVYFGALYTLALGTGGTKPNISTIGADQFDD 60
           ++V++P ++PP C+     +CEKAS LQLAV+FGALYTLA+GTGGTKPNISTIGADQFD 
Sbjct: 118 LSVTIPGIKPPECSTTNVENCEKASVLQLAVFFGALYTLAIGTGGTKPNISTIGADQFDV 177

Query: 61  FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAISIGIFVS 120
           F PKEK QKLSFFNWWMFSIFFGTLFAN +LVY+QDN+GWTLGYGLPT+GLAISI IF+ 
Sbjct: 178 FDPKEKTQKLSFFNWWMFSIFFGTLFANTVLVYVQDNVGWTLGYGLPTLGLAISITIFLL 237

Query: 121 GIPFYRHKVPAGSPFTRMAKVIVAAVRKRKLPLPQEAKELYELDLEEYTKKRKFRMEATP 180
           G PFYRHK+P GSPFT+MA+VIVA+ RK   P+  +    +EL   EY +K  F +  TP
Sbjct: 238 GTPFYRHKLPTGSPFTKMARVIVASFRKANAPMTHDITSFHELPSLEYERKGAFPIHPTP 297

Query: 181 SLSFLNKASIKTGSTDPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG 240
           SL FL++AS+KTG+   W LCT TEVEETKQM+RM+P+LF TFVPS+MLAQ+NTLF+KQG
Sbjct: 298 SLRFLDRASLKTGTNHKWNLCTTTEVEETKQMLRMLPVLFITFVPSMMLAQINTLFVKQG 357

Query: 241 TTLDRKI-GNFNIPPASLSGFVTASLLISVVLYDRVFVKIMRKFTKNPRGITLLQRMGIG 300
           TTLDRK+ G+F+IPPASLSGFVT S+LIS+VLYDRVFVKI RKFT NPRGITLLQRMGIG
Sbjct: 358 TTLDRKVTGSFSIPPASLSGFVTLSMLISIVLYDRVFVKITRKFTGNPRGITLLQRMGIG 417

Query: 301 IVLHMLIMVVCSLVERRRLTVAREHGVV-QSGGQVPLSIFILLPQFILMGTADAFMEVSK 360
           ++ H+LIM+V S+ ER RL VA +HG++ Q+G ++PL+IF LLPQF+LMG AD+F+EV+K
Sbjct: 418 LIFHILIMIVASVTERYRLKVAADHGLIHQTGVKLPLTIFALLPQFVLMGMADSFLEVAK 477

Query: 361 IEFFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVSDLTRRHGRKGWILNNLNASHL 420
           +EFFYDQAPESMKSLGTSYSTT++  GNF+SSFLLSTVS++T++ GR GWILNNLN S L
Sbjct: 478 LEFFYDQAPESMKSLGTSYSTTSLAIGNFMSSFLLSTVSEITKKRGR-GWILNNLNESRL 537

Query: 421 DYYYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEELKE 463
           DYYY FFA+LN +NF+ FLVV ++YVY+AEV+DS+ +   E+KE
Sbjct: 538 DYYYLFFAVLNLVNFVLFLVVVKFYVYRAEVTDSVDVKEVEMKE 580

BLAST of HG10010134 vs. TAIR 10
Match: AT5G46040.1 (Major facilitator superfamily protein )

HSP 1 Score: 603.6 bits (1555), Expect = 1.4e-172
Identity = 301/461 (65.29%), Postives = 375/461 (81.34%), Query Frame = 0

Query: 1   MAVSLPTLRPPPCAEPTSGHCEKASTLQLAVYFGALYTLALGTGGTKPNISTIGADQFDD 60
           ++VSLP L+PP C+     +CEKAS +QLAV+FGALYTLA+GTGGTKPNISTIGADQFD+
Sbjct: 118 LSVSLPGLKPPKCSTANVENCEKASVIQLAVFFGALYTLAIGTGGTKPNISTIGADQFDE 177

Query: 61  FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAISIGIFVS 120
           F PK+K  K SFFNWWMFSIFFGT FA  +LVY+QDN+GW +GYGL T+GLA SI IF+ 
Sbjct: 178 FDPKDKIHKHSFFNWWMFSIFFGTFFATTVLVYVQDNVGWAIGYGLSTLGLAFSIFIFLL 237

Query: 121 GIPFYRHKVPAGSPFTRMAKVIVAAVRKRKLPLPQEAKELYELDLEEYTKKRKFRMEATP 180
           G   YRHK+P GSPFT+MA+VIVA++RK + P+  ++   YEL   EY  KR F + +T 
Sbjct: 238 GTRLYRHKLPMGSPFTKMARVIVASLRKAREPMSSDSTRFYELPPMEYASKRAFPIHSTS 297

Query: 181 SLSFLNKASIKTGSTDPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQG 240
           SL FLN+AS+KTGST  WRLCT+TEVEETKQM++M+P+LF TFVPS+MLAQ+ TLFIKQG
Sbjct: 298 SLRFLNRASLKTGSTHKWRLCTITEVEETKQMLKMLPVLFVTFVPSMMLAQIMTLFIKQG 357

Query: 241 TTLDRKI-GNFNIPPASLSGFVTASLLISVVLYDRVFVKIMRKFTKNPRGITLLQRMGIG 300
           TTLDR++  NF+IPPASL GF T S+L+S+V+YDRVFVK MRK T NPRGITLLQRMGIG
Sbjct: 358 TTLDRRLTNNFSIPPASLLGFTTFSMLVSIVIYDRVFVKFMRKLTGNPRGITLLQRMGIG 417

Query: 301 IVLHMLIMVVCSLVERRRLTVAREHGVV-QSGGQVPLSIFILLPQFILMGTADAFMEVSK 360
           ++LH+LIM++ S+ ER RL VA EHG+  Q+   +PLSIF LLPQ++LMG ADAF+E++K
Sbjct: 418 MILHILIMIIASITERYRLKVAAEHGLTHQTAVPIPLSIFTLLPQYVLMGLADAFIEIAK 477

Query: 361 IEFFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVSDLTRRHGRKGWILNNLNASHL 420
           +EFFYDQAPESMKSLGTSY++T++  G F+SS LLS+VS +T++ GR GWI NNLN S L
Sbjct: 478 LEFFYDQAPESMKSLGTSYTSTSMAVGYFMSSILLSSVSQITKKQGR-GWIQNNLNESRL 537

Query: 421 DYYYGFFAILNFLNFIFFLVVSRYYVYKAEVSDSIRLLTEE 460
           D YY FFA+LN LNFI FLVV R+Y Y+A+V+ S  +  +E
Sbjct: 538 DNYYMFFAVLNLLNFILFLVVIRFYEYRADVTQSANVEQKE 577

BLAST of HG10010134 vs. TAIR 10
Match: AT2G40460.1 (Major facilitator superfamily protein )

HSP 1 Score: 443.4 bits (1139), Expect = 2.4e-124
Identity = 234/450 (52.00%), Postives = 313/450 (69.56%), Query Frame = 0

Query: 1   MAVSLPTLRPPPCAEPTSGHCEKASTLQLAVYFGALYTLALGTGGTKPNISTIGADQFDD 60
           MAV++ +LR P C    +G C KAS+LQ+  ++ +LYT+A+G GGTKPNIST GADQFD 
Sbjct: 115 MAVTVKSLR-PTC---ENGVCNKASSLQVTFFYISLYTIAIGAGGTKPNISTFGADQFDS 174

Query: 61  FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAISIGIFVS 120
           +  +EKKQK+SFFNWWMFS F G LFA + LVYIQ+N+GW LGYG+PT+GL +S+ +F  
Sbjct: 175 YSIEEKKQKVSFFNWWMFSSFLGALFATLGLVYIQENLGWGLGYGIPTVGLLVSLVVFYI 234

Query: 121 GIPFYRHKVPAGSPFTR-MAKVIVAAVRKRKLPLPQEAKELYELDLEEYTKKRKFRMEAT 180
           G PFYRHKV       + + +V +AA + RKL  P +  ELYELD   Y    K ++  T
Sbjct: 235 GTPFYRHKVIKTDNLAKDLVQVPIAAFKNRKLQCPDDHLELYELDSHYYKSNGKHQVHHT 294

Query: 181 PSLSFLNKASIKTGSTDPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVNTLFIKQ 240
           P   FL+KA+IKT S  P   CTVT+VE  K+++ +I I   T +PS + AQVNTLF+KQ
Sbjct: 295 PVFRFLDKAAIKTSSRVP---CTVTKVEVAKRVLGLIFIWLVTLIPSTLWAQVNTLFVKQ 354

Query: 241 GTTLDRKIG-NFNIPPASLSGFVTASLLISVVLYDRVFVKIMRKFTKNPRGITLLQRMGI 300
           GTTLDRKIG NF IP ASL  FVT S+L+SV +YD+ FV  MRK T NPRGITLLQR+G+
Sbjct: 355 GTTLDRKIGSNFQIPAASLGSFVTLSMLLSVPMYDQSFVPFMRKKTGNPRGITLLQRLGV 414

Query: 301 GIVLHMLIMVVCSLVERRRLTVAREHGVVQSGGQVPLSIFILLPQFILMGTADAFMEVSK 360
           G  + ++ + + S VE +R+ V +E  +      VP+SIF LLPQ+ L+G  D F  +  
Sbjct: 415 GFAIQIVAIAIASAVEVKRMRVIKEFHITSPTQVVPMSIFWLLPQYSLLGIGDVFNAIGL 474

Query: 361 IEFFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVSDLTRRHGRKGWILNNLNASHL 420
           +EFFYDQ+PE M+SLGT++ T+ IG GNFL+SFL++ +  +T + G K WI NNLN S L
Sbjct: 475 LEFFYDQSPEEMQSLGTTFFTSGIGLGNFLNSFLVTMIDKITSKGGGKSWIGNNLNDSRL 534

Query: 421 DYYYGFFAILNFLNFIFFLVVSRYYVYKAE 449
           DYYYGF  +++ +N   F+  +  YVYK++
Sbjct: 535 DYYYGFLVVISIVNMGLFVWAASKYVYKSD 557

BLAST of HG10010134 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 366.3 bits (939), Expect = 3.7e-101
Identity = 193/455 (42.42%), Postives = 300/455 (65.93%), Query Frame = 0

Query: 1   MAVSLPTLRPPPCAEPTSGHCEKASTLQLAVYFGALYTLALGTGGTKPNISTIGADQFDD 60
           ++ S+P L+P  C       C  A+  Q A++FG LY +ALGTGG KP +S+ GADQFDD
Sbjct: 132 LSASVPALKPAEC---IGDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDD 191

Query: 61  FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAISIGIFVS 120
              +E+ +K SFFNW+ FSI  G L ++ +LV+IQ+N GW LG+G+PT+ + ++I  F  
Sbjct: 192 TDSRERVRKASFFNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFF 251

Query: 121 GIPFYRHKVPAGSPFTRMAKVIVAAVRKRKLPLPQEAKELYELDLEEYTKKRKFRMEATP 180
           G P YR + P GSP TR+++V+VA+ RK  + +P++A  LYE   +        ++E T 
Sbjct: 252 GTPLYRFQKPGGSPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTD 311

Query: 181 SLSFLNKASI------KTGS-TDPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVN 240
              +L+KA++      K+G  ++ WRLCTVT+VEE K +IRM PI  +  + S + AQ++
Sbjct: 312 DCQYLDKAAVISEEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMS 371

Query: 241 TLFIKQGTTLDRKIGNFNIPPASLSGFVTASLLISVVLYDRVFVKIMRKFTKNPRGITLL 300
           T+F++QG  ++ KIG+F +PPA+L  F TAS++I V LYDR  V + RKFT   +G T +
Sbjct: 372 TMFVQQGRAMNCKIGSFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEI 431

Query: 301 QRMGIGIVLHMLIMVVCSLVERRRLTVAREHGVVQSGGQVPLSIFILLPQFILMGTADAF 360
           QRMGIG+ + +L M   ++VE  RL +A + G+V+SG  VP+S+   +PQ+ ++G A+ F
Sbjct: 432 QRMGIGLFVSVLCMAAAAIVEIIRLHMANDLGLVESGAPVPISVLWQIPQYFILGAAEVF 491

Query: 361 MEVSKIEFFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVSDLTRRHGRKGWILNNL 420
             + ++EFFYDQ+P++M+SL ++ +      GN+LSS +L+ V+  T R+G++GWI +NL
Sbjct: 492 YFIGQLEFFYDQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNL 551

Query: 421 NASHLDYYYGFFAILNFLNF-IFFLVVSRYYVYKA 448
           N+ HLDY++   A L+ +N  ++F   +RY   KA
Sbjct: 552 NSGHLDYFFWLLAGLSLVNMAVYFFSAARYKQKKA 583

BLAST of HG10010134 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 361.3 bits (926), Expect = 1.2e-99
Identity = 198/457 (43.33%), Postives = 295/457 (64.55%), Query Frame = 0

Query: 1   MAVSLPTLRPPPCAEPTSGHCEKASTLQLAVYFGALYTLALGTGGTKPNISTIGADQFDD 60
           ++ S+P L+P  C   T   C   S+ Q AV+F ALY +ALGTGG KP +S+ GADQFD+
Sbjct: 115 LSASVPGLKPGNCNADT---CHPNSS-QTAVFFVALYMIALGTGGIKPCVSSFGADQFDE 174

Query: 61  FHPKEKKQKLSFFNWWMFSIFFGTLFANIILVYIQDNIGWTLGYGLPTIGLAISIGIFVS 120
               EK +K SFFNW+ FSI  G L A  +LV+IQ N+GW  G+G+PT+ + I++  F  
Sbjct: 175 NDENEKIKKSSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFF 234

Query: 121 GIPFYRHKVPAGSPFTRMAKVIVAAVRKRKLPLPQEAKELYELDLEEYTKKRKFRMEATP 180
           G  FYR + P GSP TR+ +VIVAA RK  + +P++   L+E   +E   K   ++  T 
Sbjct: 235 GSRFYRLQRPGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTD 294

Query: 181 SLSFLNKA-------SIKTGSTDPWRLCTVTEVEETKQMIRMIPILFATFVPSIMLAQVN 240
           +L F +KA       SIK G  +PWRLC+VT+VEE K +I ++P+     V + + +Q++
Sbjct: 295 NLKFFDKAAVESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMS 354

Query: 241 TLFIKQGTTLDRKIG-NFNIPPASLSGFVTASLLISVVLYDRVFVKIMRKFTKNPRGITL 300
           T+F+ QG T+D+ +G NF IP ASLS F T S+L    +YD+  + + RKFT+N RG T 
Sbjct: 355 TMFVLQGNTMDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQ 414

Query: 301 LQRMGIGIVLHMLIMVVCSLVERRRLTVAREHGVVQSGGQVPLSIFILLPQFILMGTADA 360
           LQRMGIG+V+ +  M+   ++E  RL   + H       Q+ +SIF  +PQ++L+G A+ 
Sbjct: 415 LQRMGIGLVVSIFAMITAGVLEVVRLDYVKTHNAYDQ-KQIHMSIFWQIPQYLLIGCAEV 474

Query: 361 FMEVSKIEFFYDQAPESMKSLGTSYSTTAIGTGNFLSSFLLSTVSDLTRRHGRKGWILNN 420
           F  + ++EFFYDQAP++M+SL ++ S T +  GN+LS+ L++ V  +T+++G+ GWI +N
Sbjct: 475 FTFIGQLEFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDN 534

Query: 421 LNASHLDYYYGFFAILNFLNFIFFLVVSRYYVYKAEV 450
           LN  HLDY++   A L+FLNF+ +L +S+ Y YK  V
Sbjct: 535 LNRGHLDYFFYLLATLSFLNFLVYLWISKRYKYKKAV 566

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038874759.11.9e-25696.41protein NRT1/ PTR FAMILY 5.2-like [Benincasa hispida][more]
XP_008460668.19.6e-25394.93PREDICTED: protein NRT1/ PTR FAMILY 5.2-like [Cucumis melo] >KAA0050720.1 protei... [more]
XP_004147160.16.9e-25194.51protein NRT1/ PTR FAMILY 5.2 [Cucumis sativus] >KGN61581.1 hypothetical protein ... [more]
XP_022138841.14.2e-24091.70protein NRT1/ PTR FAMILY 5.2-like [Momordica charantia][more]
XP_023515133.11.0e-23889.85protein NRT1/ PTR FAMILY 5.2-like isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9FNL75.2e-18569.18Protein NRT1/ PTR FAMILY 5.2 OS=Arabidopsis thaliana OX=3702 GN=NPF5.2 PE=2 SV=1[more]
Q9FNL81.9e-17165.29Protein NRT1/ PTR FAMILY 5.3 OS=Arabidopsis thaliana OX=3702 GN=NPF5.3 PE=2 SV=1[more]
Q8VZR73.3e-12352.00Protein NRT1/ PTR FAMILY 5.1 OS=Arabidopsis thaliana OX=3702 GN=NPF5.1 PE=2 SV=2[more]
P460325.2e-10042.42Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1[more]
Q9M3901.7e-9843.33Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3BRP94.6e-25394.93Protein NRT1/ PTR FAMILY 5.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3CCI54.6e-25394.93protein NRT1/ PTR FAMILY 5.2-like OS=Cucumis melo OX=3656 GN=LOC103499437 PE=3 S... [more]
A0A0A0LNM73.3e-25194.51Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G172510 PE=3 SV=1[more]
A0A6J1CAW62.0e-24091.70protein NRT1/ PTR FAMILY 5.2-like OS=Momordica charantia OX=3673 GN=LOC111009912... [more]
A0A6J1KMR21.5e-23889.85protein NRT1/ PTR FAMILY 5.2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT5G46050.13.7e-18669.18peptide transporter 3 [more]
AT5G46040.11.4e-17265.29Major facilitator superfamily protein [more]
AT2G40460.12.4e-12452.00Major facilitator superfamily protein [more]
AT2G02040.13.7e-10142.42peptide transporter 2 [more]
AT3G54140.11.2e-9943.33peptide transporter 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 5..409
e-value: 4.4E-97
score: 325.4
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 1..456
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 1..451
e-value: 2.4E-147
score: 493.3
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 29..442
NoneNo IPR availablePANTHERPTHR11654:SF453PROTEIN NRT1/ PTR FAMILY 5.2-RELATEDcoord: 1..456

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10010134.1HG10010134.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035442 dipeptide transmembrane transport
biological_process GO:0042939 tripeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0071916 dipeptide transmembrane transporter activity
molecular_function GO:0042937 tripeptide transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity