HG10010067 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10010067
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptioncytochrome b5-like
LocationChr06: 17766080 .. 17769297 (+)
RNA-Seq ExpressionHG10010067
SyntenyHG10010067
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGGCGACGGAAAGCTCTTTACTTTGGCTGAAGTCTCTCAGCACAATAACCGCAAAGACTGTTGGTTGATCATCAATGCCAAGGTTTTTCTTCATACCCTTTTCTTTGTTTTTGGGGTTTCTTCTTTCATATGTCTCATTATGCCTGTAGGAGTTTGATTCTCTGAAGTTGTTTGTTCTTGTTCTGTTTTCGTTTGCTCTTGAAATCTCACATGCATTCTGGACAGACACATTGTTCTTCACTGTTGAATCAACATGTTTATCCATGGCTGCTAAACTGCTGCATGGTGAGCTTTACCAATGATGTTGTATAGGGCTGGCTAATGAAGGAAATTAGTTCCACATTATAGTGTAAATCGAACTATTTCTTAACCTTTTTTTCTTGAGGAATGCAAAGAGATTCAGGCTTAGTTTTGGATTTCGTAGCTTTTAAAATAATTGTTGCGAATGGTGCTTTTATAAAAATGAGCTGTACTACGAAGGAAATTGGTGTTTGGTAGATCTATAACTTAAACGTGGAAAATTAGGATAAAATAGGAACTTTTAGAAGATATCATATTATTTAGCATTATATAATGGGACAGTTGCAATATAGCAAACATGCCTAAAATATTTGCAAATATAGCACCATGCAAAAGAATATATCACCAATAAGAGTCTATCATTGATAGACCATATTGCTAATAGAAGTCTATCAGCGATAGACTATATTATTGATAGATTTTGCTATATTTGCAATTTTTTTAAAATGTTGCTATACACTTAGTTATTATCCCTACTATCCATTGCAATTACCCTTATATAATCAGGTTTACCAAACATTAAATACAGTTAAGATTCCCTCTAATCACTTTTAACATTGGCAAAATCAATTCCAAATGCAGCCTCAATCCTTGGAGAGTGATGTTTCATCCTCACAAGGGCTTAGATTGGTTCTTTGGTTGGGTGGCTTTTGTGTAGAAGTTGCTAGAGACTGACTGACTGAGTAAGGCTCAAATGCCTCAGTCACATCGTTTGTTTAAGTTGAAGAGCATGAGTTTGTGTAATATGTGTTAATAGGTTATGGAAAGGGATGAGCTCTATAGATGTGTATTCATATCACGTATGTTTGGTGTAGGAGTTTGTGATCTACAAAATTTTATATATAGTGGATTATATAAGCCTGAGAATGTGTAACATTGGTTTGTGTAGTTCACGAGATTAACGAACAAATTTGCAGACGAAAATAAACAAACTCACATGATAGCAAACATAGACGTGGCTTCTTAATTCCAGATTATACATTCCTTACTCTGCTCACCTTACCAAGTTTATAAGACTGGACTGGACTGGAGATTTATGATTTATGGATCTTAAAATGGGCTGTGTTTAGCATTTTGAATGGACTTATTATGTGTTTTTGATTCCTTTTAGGACCAGGATCTCATTGAATGGATAGAATCTGAGGCTTCTTTATATTTGGCAGTCCTGTTATGAAACAATGTTCTAGTGGATTGCAGTACTGGGGACTGCTTGATCACTGTGATTTTCATATTTTTACCGATGCATTGAAAGGAATACAAATATCAGTGTAGGAAAACTCCGTTGGGTGATTTACTTTTACATCTGAATAGTGGTTAATATGAAGATCACTCACACAAAAATGAAGTCCAGATCTCATTTTATGTTTGTGTGTGTGAATGCACTTGTTTACTGAGGGCACTCTTATGCATATCTTGTGAGAACTGGACTCCGATGGGGTTGGACATCCAACACCCTGTTCAAACTTTACCAATAAAAAATCAGGTGTCTGTTTGATGAATGGTCAACTTACATCGCTGCCATTCTGTCTGGTTAATGTCAGTCACTGGAGCCCTAATCTGCCATAAAGACTTTGTTCATTCTTGATTGTACTGATTCCGTTTCATGACTTCTTCGTTTGGCATTCATAATCCGATCTCAAAGCAATATCCTGAAGCCTCACAGTCTGCTGAATTTGCACATTCCATTGATGTAAAAAATCTCCTGCCAGGCATCATACTTATGTTCAGCCCTACTAAATCTTTAAGTAGGCTTCATCCACGTAGAGGCGAGATTCATGACATATGAAGAGTTTGAAAATATTGACTTCCCCTACCTAGGCTTGAATGTTGAACCTAGACATTTCAATTATAACTTCACGTCCTTTCCACTAGAGCCATCCTTTGGGTAAAATCAGAAATTTACCGTACCGAAATTAGTAGTTATAAGCTCTTGTTGGTTGTACTTGTTCGTCATTGTGATAGAAACTCTGGTTTCTTCCTCATTAATGTAATGAGAAATCTCTTTCTTGACCCCCATATCAAAAGGATAAAGATAATTGTAGAAATGCTTGTGTTGGAGAAAGTCAAGTACTAGTTTCTCAACCCAAGCTTGAGAACCATGATCATTAGCCCACTCAAATTTCTTTTAAGTATAAAGTAAGAAGCAGGAGGATAAACATGAAAGAGCATGAATCTTCCCTCAAGTTAACCCGATTCCTAACTTGGAAGTCAATATTTGGAATTGCACAAACTATCTGCAAAGAACAACTCTTGAGCATTTCAACTATTTTTTGTTCCTGAAATTTCTTAAGAACAGTAATAGAAAATCGAACCATTTTGATGTTCTCACTAGTTGTACTATGGTTGGTCGTTCAGCCCAGAATGCGATCGACAAGCGTTCAGTTGTTTTGGCAGTTGGCCTGGAATTAGACTAAATAAGTCATGCACAGATTTTATATTTGATGGGTACTGGTTTTTAGCTTGAGGTTATCATTTTGTCCATCTTGATTATCCCAACAGGTTTACAATGTAACCGAGTTCTTGGAAGACCATCCTGGTGGCGACGAGGTGTTGTTGTCCGGCACTGGTAATAAACACTTCCAGTCATATAGCTTTAGTTCTTTTCATTATACGTCGAGTGTTTGTGCATCACTTACATATCATGTAATTTTCATTGCAACAGGTAAGGATGCAACTGATGATTTTGAAGATGTGGGTCATAGTGAAAGTGCAAGAGAAATGATGAGCAAATACTACGTTGGAGAGATCGACATCTCGACGATCCCAGAACAGACGACATACACAACTCCAAAGCAGCCTCATTACAATCAGGATAAGACGACCGAGTTCATAATCAAGCTCCTCCAGTTCTTGGTTCCTCTCGCAATCTTGGGTTTGGCAGTCGGTATCCGCTTTTACACCAAATCAACCTGA

mRNA sequence

ATGGGTGGCGACGGAAAGCTCTTTACTTTGGCTGAAGTCTCTCAGCACAATAACCGCAAAGACTGTTGGTTGATCATCAATGCCAAGGTTTACAATGTAACCGAGTTCTTGGAAGACCATCCTGGTGGCGACGAGGTGTTGTTGTCCGGCACTGGTAAGGATGCAACTGATGATTTTGAAGATGTGGGTCATAGTGAAAGTGCAAGAGAAATGATGAGCAAATACTACGTTGGAGAGATCGACATCTCGACGATCCCAGAACAGACGACATACACAACTCCAAAGCAGCCTCATTACAATCAGGATAAGACGACCGAGTTCATAATCAAGCTCCTCCAGTTCTTGGTTCCTCTCGCAATCTTGGGTTTGGCAGTCGGTATCCGCTTTTACACCAAATCAACCTGA

Coding sequence (CDS)

ATGGGTGGCGACGGAAAGCTCTTTACTTTGGCTGAAGTCTCTCAGCACAATAACCGCAAAGACTGTTGGTTGATCATCAATGCCAAGGTTTACAATGTAACCGAGTTCTTGGAAGACCATCCTGGTGGCGACGAGGTGTTGTTGTCCGGCACTGGTAAGGATGCAACTGATGATTTTGAAGATGTGGGTCATAGTGAAAGTGCAAGAGAAATGATGAGCAAATACTACGTTGGAGAGATCGACATCTCGACGATCCCAGAACAGACGACATACACAACTCCAAAGCAGCCTCATTACAATCAGGATAAGACGACCGAGTTCATAATCAAGCTCCTCCAGTTCTTGGTTCCTCTCGCAATCTTGGGTTTGGCAGTCGGTATCCGCTTTTACACCAAATCAACCTGA

Protein sequence

MGGDGKLFTLAEVSQHNNRKDCWLIINAKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPEQTTYTTPKQPHYNQDKTTEFIIKLLQFLVPLAILGLAVGIRFYTKST
Homology
BLAST of HG10010067 vs. NCBI nr
Match: XP_008457249.1 (PREDICTED: cytochrome b5-like [Cucumis melo] >KAA0033284.1 cytochrome b5-like [Cucumis melo var. makuwa] >TYK14823.1 cytochrome b5-like [Cucumis melo var. makuwa])

HSP 1 Score: 261.9 bits (668), Expect = 3.0e-66
Identity = 126/134 (94.03%), Postives = 129/134 (96.27%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNRKDCWLIINAKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGKL+TLAEVSQHNN KDCWLIIN KVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGDGKLYTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPEQTTYTTPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHSESAREMMSKYY+GEIDISTIPEQ TYT PKQPHYNQDKTTEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSESAREMMSKYYIGEIDISTIPEQLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYTKST 135
           LGLAVGIRFY KS+
Sbjct: 121 LGLAVGIRFYNKSS 134

BLAST of HG10010067 vs. NCBI nr
Match: XP_038906915.1 (cytochrome b5-like [Benincasa hispida])

HSP 1 Score: 257.7 bits (657), Expect = 5.6e-65
Identity = 126/134 (94.03%), Postives = 129/134 (96.27%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNRKDCWLIINAKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           M GD KLFTLAEVSQHNNRKDCWLIIN KVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MDGDRKLFTLAEVSQHNNRKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPEQTTYTTPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHSESAREMMSKY VGEIDISTIPE++TYTT KQPHYNQDKTTEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSESAREMMSKYSVGEIDISTIPERSTYTTSKQPHYNQDKTTEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYTKST 135
           LGLAVGIRFY+KST
Sbjct: 121 LGLAVGIRFYSKST 134

BLAST of HG10010067 vs. NCBI nr
Match: XP_004138957.1 (cytochrome b5 [Cucumis sativus] >KGN61465.1 hypothetical protein Csa_005989 [Cucumis sativus])

HSP 1 Score: 257.3 bits (656), Expect = 7.3e-65
Identity = 124/134 (92.54%), Postives = 127/134 (94.78%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNRKDCWLIINAKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGKLFT AEVSQHNN KDCWLIIN KVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGDGKLFTFAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPEQTTYTTPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHSESARE MSKYYVGEIDIST+PE+ TYT PKQPHYNQDKTTEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYTKST 135
           LGLAVGIRFY KS+
Sbjct: 121 LGLAVGIRFYNKSS 134

BLAST of HG10010067 vs. NCBI nr
Match: XP_022138846.1 (cytochrome b5-like [Momordica charantia])

HSP 1 Score: 253.4 bits (646), Expect = 1.1e-63
Identity = 121/134 (90.30%), Postives = 127/134 (94.78%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNRKDCWLIINAKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGK+FTLAEVSQHN  KDCWLIIN KVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 50  MGGDGKIFTLAEVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 109

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPEQTTYTTPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHS+SARE M+KYYVGEIDISTIPE+ TY+  KQPHYNQDKTTEFI+KLLQFLVPLAI
Sbjct: 110 DVGHSDSARETMNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAI 169

Query: 121 LGLAVGIRFYTKST 135
           LGLAVGIRFYTKST
Sbjct: 170 LGLAVGIRFYTKST 183

BLAST of HG10010067 vs. NCBI nr
Match: XP_023006481.1 (cytochrome b5-like [Cucurbita maxima])

HSP 1 Score: 252.7 bits (644), Expect = 1.8e-63
Identity = 121/134 (90.30%), Postives = 127/134 (94.78%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNRKDCWLIINAKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGG GK+FTLAEVSQHN R+DCWLIIN KVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGHGKVFTLAEVSQHNTRQDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPEQTTYTTPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHSESARE M+KYYVGEIDISTIPE+ TYT  KQPHYNQDKTTEFI+KLLQFLVPLAI
Sbjct: 61  DVGHSESARESMNKYYVGEIDISTIPERLTYTASKQPHYNQDKTTEFIVKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYTKST 135
           LGLAVGIRFY+KST
Sbjct: 121 LGLAVGIRFYSKST 134

BLAST of HG10010067 vs. ExPASy Swiss-Prot
Match: O48845 (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 2.1e-59
Identity = 107/133 (80.45%), Postives = 120/133 (90.23%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNRKDCWLIINAKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MG + K+FTL+EVS+HN   DCW++IN KVYNVT+FLEDHPGGD+VLLS TGKDATDDFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPEQTTYTTPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHSESAREMM +YYVGEID +TIP++  YT PKQPHYNQDKT+EFIIKLLQFLVPLAI
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYTKS 134
           LGLAVGIR YTKS
Sbjct: 121 LGLAVGIRIYTKS 133

BLAST of HG10010067 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 1.5e-57
Identity = 102/134 (76.12%), Postives = 119/134 (88.81%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNRKDCWLIINAKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGG+ K+FTLAEVSQHNN KDCWL+I+ KVY+VT+FL+DHPGGDEVLLS TGKDATDDFE
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPEQTTYTTPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHS SAR M+ +YYVG+ID +TIP +T YT P QPHYNQDKT+EF++KLLQFLVPL I
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYNQDKTSEFVVKLLQFLVPLII 120

Query: 121 LGLAVGIRFYTKST 135
           LG+A GIRFYTK +
Sbjct: 121 LGVAFGIRFYTKQS 134

BLAST of HG10010067 vs. ExPASy Swiss-Prot
Match: P49099 (Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 2.6e-57
Identity = 103/134 (76.87%), Postives = 115/134 (85.82%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNRKDCWLIINAKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGG  K+FTLAEVS HNN KDCWLII+ KVYNVT+FLEDHPGG EVLLS TGKDATDDFE
Sbjct: 1   MGGQSKVFTLAEVSNHNNAKDCWLIISGKVYNVTKFLEDHPGGGEVLLSATGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPEQTTYTTPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           D+GHS SAR M+ +YYVG+ID STIP +  YT PKQPHYNQDKTTEFI+KLLQFLVPL I
Sbjct: 61  DIGHSSSARAMLDEYYVGDIDSSTIPTKVKYTPPKQPHYNQDKTTEFIVKLLQFLVPLII 120

Query: 121 LGLAVGIRFYTKST 135
           LG+A G+ FYTK +
Sbjct: 121 LGVAFGVHFYTKQS 134

BLAST of HG10010067 vs. ExPASy Swiss-Prot
Match: O04354 (Cytochrome b5 OS=Borago officinalis OX=13363 PE=2 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 2.1e-54
Identity = 99/130 (76.15%), Postives = 113/130 (86.92%), Query Frame = 0

Query: 5   GKLFTLAEVSQHNNRKDCWLIINAKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGH 64
           GK+FTLAEV+QHNN KDCWLIIN KVY+VT+FLEDHPGGD+VLLS TGKDATDDFED+GH
Sbjct: 2   GKIFTLAEVAQHNNSKDCWLIINGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFEDIGH 61

Query: 65  SESAREMMSKYYVGEIDISTIPEQTTYTTPKQPHYNQDKTTEFIIKLLQFLVPLAILGLA 124
           S SA+ M+ +YYVG+ID S+IP Q  YT PKQP YN DKT EF+IKLLQFLVPL IL  A
Sbjct: 62  SSSAKAMLDEYYVGDIDSSSIPSQVKYTPPKQPLYNPDKTREFVIKLLQFLVPLVILAGA 121

Query: 125 VGIRFYTKST 135
           +GIRFYTKS+
Sbjct: 122 IGIRFYTKSS 131

BLAST of HG10010067 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 210.3 bits (534), Expect = 1.3e-53
Identity = 98/128 (76.56%), Postives = 112/128 (87.50%), Query Frame = 0

Query: 6   KLFTLAEVSQHNNRKDCWLIINAKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHS 65
           K++TL EV++HN++ DCWLII  KVYNV++FLEDHPGGD+VLLS TGKDATDDFEDVGHS
Sbjct: 7   KVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFEDVGHS 66

Query: 66  ESAREMMSKYYVGEIDISTIPEQTTYTTPKQPHYNQDKTTEFIIKLLQFLVPLAILGLAV 125
            +AR MM +YYVG+ID STIP +T Y  PKQPHYNQDKT EFIIK+LQFLVPLAILGLAV
Sbjct: 67  TTARAMMDEYYVGDIDTSTIPARTKYVPPKQPHYNQDKTPEFIIKILQFLVPLAILGLAV 126

Query: 126 GIRFYTKS 134
            IR YTKS
Sbjct: 127 AIRIYTKS 134

BLAST of HG10010067 vs. ExPASy TrEMBL
Match: A0A5D3CSF5 (Cytochrome b5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold472G00160 PE=3 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 1.4e-66
Identity = 126/134 (94.03%), Postives = 129/134 (96.27%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNRKDCWLIINAKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGKL+TLAEVSQHNN KDCWLIIN KVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGDGKLYTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPEQTTYTTPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHSESAREMMSKYY+GEIDISTIPEQ TYT PKQPHYNQDKTTEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSESAREMMSKYYIGEIDISTIPEQLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYTKST 135
           LGLAVGIRFY KS+
Sbjct: 121 LGLAVGIRFYNKSS 134

BLAST of HG10010067 vs. ExPASy TrEMBL
Match: A0A1S3C5R9 (cytochrome b5-like OS=Cucumis melo OX=3656 GN=LOC103496974 PE=3 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 1.4e-66
Identity = 126/134 (94.03%), Postives = 129/134 (96.27%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNRKDCWLIINAKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGKL+TLAEVSQHNN KDCWLIIN KVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGDGKLYTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPEQTTYTTPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHSESAREMMSKYY+GEIDISTIPEQ TYT PKQPHYNQDKTTEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSESAREMMSKYYIGEIDISTIPEQLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYTKST 135
           LGLAVGIRFY KS+
Sbjct: 121 LGLAVGIRFYNKSS 134

BLAST of HG10010067 vs. ExPASy TrEMBL
Match: A0A0A0LHS9 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G138200 PE=3 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 3.5e-65
Identity = 124/134 (92.54%), Postives = 127/134 (94.78%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNRKDCWLIINAKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGKLFT AEVSQHNN KDCWLIIN KVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGDGKLFTFAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPEQTTYTTPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHSESARE MSKYYVGEIDIST+PE+ TYT PKQPHYNQDKTTEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYTKST 135
           LGLAVGIRFY KS+
Sbjct: 121 LGLAVGIRFYNKSS 134

BLAST of HG10010067 vs. ExPASy TrEMBL
Match: A0A6J1CAX1 (cytochrome b5-like OS=Momordica charantia OX=3673 GN=LOC111009917 PE=3 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 5.1e-64
Identity = 121/134 (90.30%), Postives = 127/134 (94.78%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNRKDCWLIINAKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGK+FTLAEVSQHN  KDCWLIIN KVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 50  MGGDGKIFTLAEVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 109

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPEQTTYTTPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHS+SARE M+KYYVGEIDISTIPE+ TY+  KQPHYNQDKTTEFI+KLLQFLVPLAI
Sbjct: 110 DVGHSDSARETMNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAI 169

Query: 121 LGLAVGIRFYTKST 135
           LGLAVGIRFYTKST
Sbjct: 170 LGLAVGIRFYTKST 183

BLAST of HG10010067 vs. ExPASy TrEMBL
Match: A0A6J1KXV9 (cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111499200 PE=3 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 8.7e-64
Identity = 121/134 (90.30%), Postives = 127/134 (94.78%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNRKDCWLIINAKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGG GK+FTLAEVSQHN R+DCWLIIN KVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGHGKVFTLAEVSQHNTRQDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPEQTTYTTPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHSESARE M+KYYVGEIDISTIPE+ TYT  KQPHYNQDKTTEFI+KLLQFLVPLAI
Sbjct: 61  DVGHSESARESMNKYYVGEIDISTIPERLTYTASKQPHYNQDKTTEFIVKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYTKST 135
           LGLAVGIRFY+KST
Sbjct: 121 LGLAVGIRFYSKST 134

BLAST of HG10010067 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 229.6 bits (584), Expect = 1.5e-60
Identity = 107/133 (80.45%), Postives = 120/133 (90.23%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNRKDCWLIINAKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MG + K+FTL+EVS+HN   DCW++IN KVYNVT+FLEDHPGGD+VLLS TGKDATDDFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPEQTTYTTPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHSESAREMM +YYVGEID +TIP++  YT PKQPHYNQDKT+EFIIKLLQFLVPLAI
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYTKS 134
           LGLAVGIR YTKS
Sbjct: 121 LGLAVGIRIYTKS 133

BLAST of HG10010067 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 195.7 bits (496), Expect = 2.4e-50
Identity = 89/132 (67.42%), Postives = 109/132 (82.58%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNRKDCWLIINAKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           M  D K+ +  EVS+HN  KDCWLII+ KVY+VT F++DHPGGDEVLLS TGKDAT+DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPEQTTYTTPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHS++AR+MM KY++GEID S++P   TY  P+QP YNQDKT EFIIK+LQFLVP+ I
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPILI 120

Query: 121 LGLAVGIRFYTK 133
           LGLA+ +R YTK
Sbjct: 121 LGLALVVRHYTK 132

BLAST of HG10010067 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 190.3 bits (482), Expect = 1.0e-48
Identity = 86/135 (63.70%), Postives = 113/135 (83.70%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNRKDCWLIINAKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGK+FTL+EVSQH++ KDCW++I+ KVY+VT+FL+DHPGGDEV+L+ TGKDATDDFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  DVGHSESAREMMSKYYVGEIDISTIPEQTTYTTPKQPH--YNQDKTTEFIIKLLQFLVPL 120
           DVGHS +A+ M+ +YYVG+ID +T+P +  +  P        QDK+++F+IKLLQFLVPL
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPL 120

Query: 121 AILGLAVGIRFYTKS 134
            ILGLA GIR+YTK+
Sbjct: 121 LILGLAFGIRYYTKT 135

BLAST of HG10010067 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 127.1 bits (318), Expect = 1.1e-29
Identity = 59/126 (46.83%), Postives = 88/126 (69.84%), Query Frame = 0

Query: 7   LFTLAEVSQHNNRKDCWLIINAKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHSE 66
           L +  +V++H  + DCW++I+ KVY+++ F+++HPGGD VLL+ TGKDA+ DFEDV HS+
Sbjct: 4   LISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSK 63

Query: 67  SAREMMSKYYVGEIDISTIPEQTTYTTPKQPHYNQDKTT--EFIIKLLQFLVPLAILGLA 126
            A+E+M KY +G++D ST+P    Y  P +      +TT  E   KLL +L+PL ILG+A
Sbjct: 64  DAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILGVA 123

Query: 127 VGIRFY 131
             +RFY
Sbjct: 124 FALRFY 129

BLAST of HG10010067 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 125.2 bits (313), Expect = 4.0e-29
Identity = 55/127 (43.31%), Postives = 91/127 (71.65%), Query Frame = 0

Query: 6   KLFTLAEVSQHNNRKDCWLIINAKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHS 65
           KL+++ E + HN + DCW++I+ KVY+V+ ++++HPGGD+VLL+  GKDATDDFED GHS
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 66  ESAREMMSKYYVGEIDISTIPEQTTYTTPKQPHYNQDKTTEFIIKLLQ-----FLVPLAI 125
           + ARE+M KY++GE+D S++PE      P+   Y +D+  + + KL       ++VP++I
Sbjct: 66  KDARELMEKYFIGELDESSLPE-----IPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSI 125

Query: 126 LGLAVGI 128
           + ++V +
Sbjct: 126 ITISVAV 127

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008457249.13.0e-6694.03PREDICTED: cytochrome b5-like [Cucumis melo] >KAA0033284.1 cytochrome b5-like [C... [more]
XP_038906915.15.6e-6594.03cytochrome b5-like [Benincasa hispida][more]
XP_004138957.17.3e-6592.54cytochrome b5 [Cucumis sativus] >KGN61465.1 hypothetical protein Csa_005989 [Cuc... [more]
XP_022138846.11.1e-6390.30cytochrome b5-like [Momordica charantia][more]
XP_023006481.11.8e-6390.30cytochrome b5-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O488452.1e-5980.45Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1[more]
P490981.5e-5776.12Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
P490992.6e-5776.87Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
O043542.1e-5476.15Cytochrome b5 OS=Borago officinalis OX=13363 PE=2 SV=1[more]
P491001.3e-5376.56Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A5D3CSF51.4e-6694.03Cytochrome b5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold472G0... [more]
A0A1S3C5R91.4e-6694.03cytochrome b5-like OS=Cucumis melo OX=3656 GN=LOC103496974 PE=3 SV=1[more]
A0A0A0LHS93.5e-6592.54Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ... [more]
A0A6J1CAX15.1e-6490.30cytochrome b5-like OS=Momordica charantia OX=3673 GN=LOC111009917 PE=3 SV=1[more]
A0A6J1KXV98.7e-6490.30cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111499200 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G32720.11.5e-6080.45cytochrome B5 isoform B [more]
AT5G53560.12.4e-5067.42cytochrome B5 isoform E [more]
AT5G48810.11.0e-4863.70cytochrome B5 isoform D [more]
AT2G46650.11.1e-2946.83cytochrome B5 isoform C [more]
AT1G26340.14.0e-2943.31cytochrome B5 isoform A [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001199Cytochrome b5-like heme/steroid binding domainPRINTSPR00363CYTOCHROMEB5coord: 68..80
score: 39.04
coord: 40..54
score: 53.54
coord: 30..40
score: 61.02
coord: 55..62
score: 48.11
IPR001199Cytochrome b5-like heme/steroid binding domainSMARTSM01117Cyt_b5_2coord: 8..81
e-value: 1.4E-33
score: 127.5
IPR001199Cytochrome b5-like heme/steroid binding domainPFAMPF00173Cyt-b5coord: 9..80
e-value: 1.1E-26
score: 92.7
IPR001199Cytochrome b5-like heme/steroid binding domainPROSITEPS50255CYTOCHROME_B5_2coord: 5..81
score: 29.152353
IPR036400Cytochrome b5-like heme/steroid binding domain superfamilyGENE3D3.10.120.10coord: 3..83
e-value: 1.7E-34
score: 119.7
IPR036400Cytochrome b5-like heme/steroid binding domain superfamilySUPERFAMILY55856Cytochrome b5-like heme/steroid binding domaincoord: 6..91
NoneNo IPR availablePANTHERPTHR19359CYTOCHROME B5coord: 1..132
NoneNo IPR availablePANTHERPTHR19359:SF110BNAC04G43300D PROTEINcoord: 1..132
IPR018506Cytochrome b5, heme-binding sitePROSITEPS00191CYTOCHROME_B5_1coord: 36..43

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10010067.1HG10010067.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043447 alkane biosynthetic process
biological_process GO:0042761 very long-chain fatty acid biosynthetic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0020037 heme binding
molecular_function GO:0046872 metal ion binding