HG10010041 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10010041
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionSerine hydroxymethyltransferase
LocationChr06: 17448090 .. 17450578 (-)
RNA-Seq ExpressionHG10010041
SyntenyHG10010041
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCCGTTAGTTCTTGGGGCAACACTCCTCTCAACACCGTCGACCCTGAGATTTTCGATCTCATCGAGAAGGAGAAGCGTCGACAATGCCGCGGAATCGAGCTCATTGCTTCTGAGAATTTCACTTCCTTCGCCGTCATCGAGGCCCTCGGCAGCGCCTTGACCAACAAGTACTCCGAGGGTATGCCGGGAAATCGATACTACGGTGGAAACGAGTTCATCGACGAGATCGAGAATCTCTGCCGTTCTCGAGCTCTCGAGGCCTTCCATGTCGATCCGGCGAAATGGGGCGTTAATGTCCAGCCCTACTCTGGTTCTCCGGCGAATTTCGCCGCTTACACCGCTTTGCTTCAGCCACATGACCGGATTATGGGGCTGGATCTTCCTTCTGGTGGCCATTTGACGCATGGTTACTACACCTCCGGTGGAAAGAAGATCTCCGCTACCTCGATTTACTTCGAGAGTTTGCCTTACAAGGTTGATTCCACCACTGGATATATTGATTACGATAAGTTGGAGGAGAAGGCTTTGGATTTCAGGCCGAAGTTGATTATCTGTGGTGGCAGTGCGTACCCGAGGGACTGGGATTATGCCAGATTCAGGGCAATTGCTGATAAATGCGGTGCTCTTCTTCTCTGTGATATGGCTCACATTAGTGGACTTGTTGCTGCTCAGGTATATATATAAACAAACGAAATCTATGGATTGAGTTCAGATCTTTTGTCATTTTGTCTTCCGGATGTTGGCAATTAGATGTTGTTTTCCATTTCTGGATTTGGGATCTGCTAGTTTATGTTGAATTTGCTTTCTCTTTGATCTATATGCAAATGATATGCTTGATTTGTTCATACGTCTCCTTCGTATCTGTGTTCAGTGTTCAGTGTTCAGTGCATTGATTAGTAATGGTTAGAAATGACACAGGTTTTTAGATCCATAATTGTTTGAGCATCTTACGTAACCGAATCTAGATTTGTAAAAAAACTTATTTAATGTCTAATTATCTATGCTACATTGCTTCTTTGTAATGGATTGTGTTCTTTGGATTGATAGAACCGTTATGTTTTTCATTGTTTCCGACAAATATTTTGCATTCTTTGTCTTTAAGATCCAGTTTTAAATGCTAGATTAAAATTGAAATGAAGATCTACTTAGAGGAAATTAATACATTTCTGCTTCTTGTAGAATTCAGTTTCAATGTAAAAAAGTCCTGTATTCAATAATCTTTTTGTTTTGGACTTACATTACGGTGTCGCTTGAATCTTTAGTATTCTCTTATTTATTACACGTGTGATAGTATTTAGGCAGAATTTGGTAATTCATTGTAACTATAAAACTGAAGGCATATTTCTTCTAAGACATTATGATTGTTTATGATCTCTGTTTCTTTCCTTGCAACTTAAGCAATTTGGGTCTGATTGGTATTGTAAAATATTAATGTCCAATGATTGTTTGCAGGAAGCTGCAAACCCGTTCGAGTATTGTGATGTCGTGACGACCACAACTCACAAGAGTTTGAGGGGTCCGAGGGCTGGTATGATCTTCTATAGGAAGGGTCCAAAGCCACCTAAGAAGGGTCAGCCTGAGGATGCTGTTTATGACTTTGAAGACAAGATCAACTTCTCTGTCTTCCCAGCCCTTCAGGGTGGCCCTCACAATCACCAAATTGGTGCTTTAGCCGTTGCCTTGAAGCAGGCCATGTCCCCCGGGTTCAAGGCCTATGCAAAGCAAGTTAAGGCCAATGCCGTGGCCCTCGGAAATTACCTGATGAACAAGGGTTACAAGCTTGTTACGGGAGGAACCGAGAACCACCTTGTTCTCTGGGATCTTCGTCCTTTGGGATTGACTGGTAATTTCATGGCGAAACAACTTATGAAAAAACTAACATGACATAGAAGTTTTAACAGTTTCTCTCGGCATCTGTCTCTTACGCCATTTCATATTGAGAAGCGGTCTTTCTGTTTTGGTCCTGTAATAACAATGTTGTGGTAATTCATTTTGTAGGCAACAAGGTTGAGAAGCTTTGCGACTTGTGCAACATCACCGTAAACAAAAATGCAGTGTTCGGCGACAGCAGTGCATTGGCTCCCGGAGGTGTTCGAATTGGTAAGTACCGCGATATCGTTGGAGGATGATTCCATCATTCTTAGTCCTTCCTTAAATTGTTTTTCCTGCATTCAAACAAATGAGTAAAAATTTCAAAAGTGTGATTAACAAGACATCAAATTGTGATGATATAATTAGGTGCACCTGCCATGACTTCAAGAGGTTTGGTTGAGAAAGACTTCGAACAGATCGCGGAGTTCCTTCACCGTGCTGTAACCATCACCTTGAATGTCCAGAAGGAATTTGGAAAGCTGTTGAAGGACTTCAACAAGGGTCTGGTAAACAACAAGGAAATCGAAGAGCTCAAGGCCGATGTCGAGAAATTTTCCGGCTCTTTCGACATGCCGGGTTTCCTCATGTCCGAGATGAAGTACAAGGACTAG

mRNA sequence

ATGGATTCCGTTAGTTCTTGGGGCAACACTCCTCTCAACACCGTCGACCCTGAGATTTTCGATCTCATCGAGAAGGAGAAGCGTCGACAATGCCGCGGAATCGAGCTCATTGCTTCTGAGAATTTCACTTCCTTCGCCGTCATCGAGGCCCTCGGCAGCGCCTTGACCAACAAGTACTCCGAGGGTATGCCGGGAAATCGATACTACGGTGGAAACGAGTTCATCGACGAGATCGAGAATCTCTGCCGTTCTCGAGCTCTCGAGGCCTTCCATGTCGATCCGGCGAAATGGGGCGTTAATGTCCAGCCCTACTCTGGTTCTCCGGCGAATTTCGCCGCTTACACCGCTTTGCTTCAGCCACATGACCGGATTATGGGGCTGGATCTTCCTTCTGGTGGCCATTTGACGCATGGTTACTACACCTCCGGTGGAAAGAAGATCTCCGCTACCTCGATTTACTTCGAGAGTTTGCCTTACAAGGTTGATTCCACCACTGGATATATTGATTACGATAAGTTGGAGGAGAAGGCTTTGGATTTCAGGCCGAAGTTGATTATCTGTGGTGGCAGTGCGTACCCGAGGGACTGGGATTATGCCAGATTCAGGGCAATTGCTGATAAATGCGGTGCTCTTCTTCTCTGTGATATGGCTCACATTAGTGGACTTGTTGCTGCTCAGGAAGCTGCAAACCCGTTCGAGTATTGTGATGTCGTGACGACCACAACTCACAAGAGTTTGAGGGGTCCGAGGGCTGGTATGATCTTCTATAGGAAGGGTCCAAAGCCACCTAAGAAGGGTCAGCCTGAGGATGCTGTTTATGACTTTGAAGACAAGATCAACTTCTCTGTCTTCCCAGCCCTTCAGGGTGGCCCTCACAATCACCAAATTGGTGCTTTAGCCGTTGCCTTGAAGCAGGCCATGTCCCCCGGGTTCAAGGCCTATGCAAAGCAAGTTAAGGCCAATGCCGTGGCCCTCGGAAATTACCTGATGAACAAGGGTTACAAGCTTGTTACGGGAGGAACCGAGAACCACCTTGTTCTCTGGGATCTTCGTCCTTTGGGATTGACTGGCAACAAGGTTGAGAAGCTTTGCGACTTGTGCAACATCACCGTAAACAAAAATGCAGTGTTCGGCGACAGCAGTGCATTGGCTCCCGGAGGTGTTCGAATTGGTGCACCTGCCATGACTTCAAGAGGTTTGGTTGAGAAAGACTTCGAACAGATCGCGGAGTTCCTTCACCGTGCTGTAACCATCACCTTGAATGTCCAGAAGGAATTTGGAAAGCTGTTGAAGGACTTCAACAAGGGTCTGGTAAACAACAAGGAAATCGAAGAGCTCAAGGCCGATGTCGAGAAATTTTCCGGCTCTTTCGACATGCCGGGTTTCCTCATGTCCGAGATGAAGTACAAGGACTAG

Coding sequence (CDS)

ATGGATTCCGTTAGTTCTTGGGGCAACACTCCTCTCAACACCGTCGACCCTGAGATTTTCGATCTCATCGAGAAGGAGAAGCGTCGACAATGCCGCGGAATCGAGCTCATTGCTTCTGAGAATTTCACTTCCTTCGCCGTCATCGAGGCCCTCGGCAGCGCCTTGACCAACAAGTACTCCGAGGGTATGCCGGGAAATCGATACTACGGTGGAAACGAGTTCATCGACGAGATCGAGAATCTCTGCCGTTCTCGAGCTCTCGAGGCCTTCCATGTCGATCCGGCGAAATGGGGCGTTAATGTCCAGCCCTACTCTGGTTCTCCGGCGAATTTCGCCGCTTACACCGCTTTGCTTCAGCCACATGACCGGATTATGGGGCTGGATCTTCCTTCTGGTGGCCATTTGACGCATGGTTACTACACCTCCGGTGGAAAGAAGATCTCCGCTACCTCGATTTACTTCGAGAGTTTGCCTTACAAGGTTGATTCCACCACTGGATATATTGATTACGATAAGTTGGAGGAGAAGGCTTTGGATTTCAGGCCGAAGTTGATTATCTGTGGTGGCAGTGCGTACCCGAGGGACTGGGATTATGCCAGATTCAGGGCAATTGCTGATAAATGCGGTGCTCTTCTTCTCTGTGATATGGCTCACATTAGTGGACTTGTTGCTGCTCAGGAAGCTGCAAACCCGTTCGAGTATTGTGATGTCGTGACGACCACAACTCACAAGAGTTTGAGGGGTCCGAGGGCTGGTATGATCTTCTATAGGAAGGGTCCAAAGCCACCTAAGAAGGGTCAGCCTGAGGATGCTGTTTATGACTTTGAAGACAAGATCAACTTCTCTGTCTTCCCAGCCCTTCAGGGTGGCCCTCACAATCACCAAATTGGTGCTTTAGCCGTTGCCTTGAAGCAGGCCATGTCCCCCGGGTTCAAGGCCTATGCAAAGCAAGTTAAGGCCAATGCCGTGGCCCTCGGAAATTACCTGATGAACAAGGGTTACAAGCTTGTTACGGGAGGAACCGAGAACCACCTTGTTCTCTGGGATCTTCGTCCTTTGGGATTGACTGGCAACAAGGTTGAGAAGCTTTGCGACTTGTGCAACATCACCGTAAACAAAAATGCAGTGTTCGGCGACAGCAGTGCATTGGCTCCCGGAGGTGTTCGAATTGGTGCACCTGCCATGACTTCAAGAGGTTTGGTTGAGAAAGACTTCGAACAGATCGCGGAGTTCCTTCACCGTGCTGTAACCATCACCTTGAATGTCCAGAAGGAATTTGGAAAGCTGTTGAAGGACTTCAACAAGGGTCTGGTAAACAACAAGGAAATCGAAGAGCTCAAGGCCGATGTCGAGAAATTTTCCGGCTCTTTCGACATGCCGGGTTTCCTCATGTCCGAGATGAAGTACAAGGACTAG

Protein sequence

MDSVSSWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDFRPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALAVALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITLNVQKEFGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD
Homology
BLAST of HG10010041 vs. NCBI nr
Match: XP_038875387.1 (serine hydroxymethyltransferase 4 [Benincasa hispida])

HSP 1 Score: 960.7 bits (2482), Expect = 4.7e-276
Identity = 465/471 (98.73%), Postives = 469/471 (99.58%), Query Frame = 0

Query: 1   MDSVSSWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+SV++WGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MESVNAWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRAL+AFHVDPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIG PAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGTPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEFGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           NVQKE GKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD
Sbjct: 421 NVQKEHGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 471

BLAST of HG10010041 vs. NCBI nr
Match: XP_004138967.1 (serine hydroxymethyltransferase 4 [Cucumis sativus] >KGN61489.1 hypothetical protein Csa_006275 [Cucumis sativus])

HSP 1 Score: 955.3 bits (2468), Expect = 2.0e-274
Identity = 461/471 (97.88%), Postives = 467/471 (99.15%), Query Frame = 0

Query: 1   MDSVSSWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MD V+ WGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRAL+AFH DPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDS TGYIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYD+EDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAM+PGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMAPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEFGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           NVQKE+GKLLKDFNKGLVNNKEIE+LKADVEKFSGSFDMPGFLMSEMKYKD
Sbjct: 421 NVQKEYGKLLKDFNKGLVNNKEIEKLKADVEKFSGSFDMPGFLMSEMKYKD 471

BLAST of HG10010041 vs. NCBI nr
Match: XP_008457222.1 (PREDICTED: serine hydroxymethyltransferase 4 [Cucumis melo] >KAA0033337.1 serine hydroxymethyltransferase 4 [Cucumis melo var. makuwa] >TYK21570.1 serine hydroxymethyltransferase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 954.9 bits (2467), Expect = 2.6e-274
Identity = 461/471 (97.88%), Postives = 466/471 (98.94%), Query Frame = 0

Query: 1   MDSVSSWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MD V+ WGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRAL+AF  DP KWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFRCDPTKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYD+EDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEFGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           N+QKE+GKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD
Sbjct: 421 NIQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 471

BLAST of HG10010041 vs. NCBI nr
Match: XP_022138598.1 (serine hydroxymethyltransferase 4 [Momordica charantia])

HSP 1 Score: 949.1 bits (2452), Expect = 1.4e-272
Identity = 458/471 (97.24%), Postives = 464/471 (98.51%), Query Frame = 0

Query: 1   MDSVSSWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+ V++WGNTPL  VDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MEPVNAWGNTPLKAVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRALEAFH DPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHADPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDS TGYIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEFGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           N+QKE+GKLLKDFNKGLVNNK+IE LKADVEKFSGSFDMPGF MSEMKYKD
Sbjct: 421 NIQKEYGKLLKDFNKGLVNNKDIEGLKADVEKFSGSFDMPGFAMSEMKYKD 471

BLAST of HG10010041 vs. NCBI nr
Match: XP_022959093.1 (serine hydroxymethyltransferase 4-like [Cucurbita moschata] >KAG6574849.1 Serine hydroxymethyltransferase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 946.8 bits (2446), Expect = 7.0e-272
Identity = 459/471 (97.45%), Postives = 464/471 (98.51%), Query Frame = 0

Query: 1   MDSVSSWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MD V++WGNTPL  VDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDS TG+IDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPE+AVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFK YAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEFGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           NVQKE GKLLKDFNKGLVNNKEIE LKADVEKFSGSF+MPGFLMSEMKYKD
Sbjct: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 471

BLAST of HG10010041 vs. ExPASy Swiss-Prot
Match: O23254 (Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 SV=1)

HSP 1 Score: 901.4 bits (2328), Expect = 4.4e-261
Identity = 433/471 (91.93%), Postives = 455/471 (96.60%), Query Frame = 0

Query: 1   MDSVSSWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+ VSSWGNT L +VDPEI DLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MEPVSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EG+PGNRYYGGNEFIDEIENLCRSRALEAFH DPA WGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGIPGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKV+ TTGYIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKL+ICGGSAYPRDWDYARFRAIADK GALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPE AVYDFEDKINF+VFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEGAVYDFEDKINFAVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQA +PGFK YAKQVKANAVALGNYLM+KGY++VT GTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLC+IT+NKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQI EFL RAVT+TL
Sbjct: 361 EKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTLTL 420

Query: 421 NVQKEFGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           ++QK +GKLLKDFNKGLVNNK++++LKADVEKFS S++MPGFLMSEMKYKD
Sbjct: 421 DIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPGFLMSEMKYKD 471

BLAST of HG10010041 vs. ExPASy Swiss-Prot
Match: Q9SVM4 (Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 1.4e-235
Identity = 384/468 (82.05%), Postives = 430/468 (91.88%), Query Frame = 0

Query: 1   MDSVSSWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+ V SWGNT L+ VDPEI+DLIEKEK RQCRGIELIA+ENFTS AV+EALGS LTNKYS
Sbjct: 1   MEPVYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGG EFIDEIE+LCRSR+LEAFH +P KWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGH+THGYY+SGGK ISATSIYFE+LPYKVDS TGYIDYDKLEEKA+DF
Sbjct: 121 HDRIMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGG++YPR+WDYARFRA+ADK GA LLCDMAH S LVAAQEAA+PFEYCDVVTT
Sbjct: 181 RPKLIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           +THKSLRGPRAGMIFYRKGPKP KKGQPE  VYDF+ KIN +VFPALQ GPHN++IGALA
Sbjct: 241 STHKSLRGPRAGMIFYRKGPKPAKKGQPEGEVYDFDAKINSAVFPALQSGPHNNKIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQ M+P FK YAKQVKANA  L +YL+NKGY LVT GT+NHL+LWDLRPLGLTGNKV
Sbjct: 301 VALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EK+C+LC IT+N+NAVFGD+S LAPGGVRIG PAMTSRGLVEKDFE+I EFLHRAVTITL
Sbjct: 361 EKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTITL 420

Query: 421 NVQKEFGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMK 469
           ++Q+++GK++KDFNKGLVNNKEI+E+KADVE+F+  FDMPGF +SE +
Sbjct: 421 DIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPGFFISESR 468

BLAST of HG10010041 vs. ExPASy Swiss-Prot
Match: Q84WV0 (Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 2.5e-179
Identity = 295/467 (63.17%), Postives = 371/467 (79.44%), Query Frame = 0

Query: 3   SVSSWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEG 62
           +V +WG+ P++  DP+I +L+EKEK+RQ RGIELIASENF   AV+EALGS LTNKYSEG
Sbjct: 129 AVRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSHLTNKYSEG 188

Query: 63  MPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHD 122
           MPG RYY GN++ID+IENLC  RAL AF ++  KWGVNVQPYS + ANFA YT LL P +
Sbjct: 189 MPGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAVYTGLLLPGE 248

Query: 123 RIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDFRP 182
           RIMGLD PSGGH++HGY T GGKKISA SI+FES PYKV+  TGYIDYDKLE+KALD+RP
Sbjct: 249 RIMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKLEDKALDYRP 308

Query: 183 KLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTT 242
           K++ICGGS+YPRDWD+AR R IADKCGA+L+CDMAHISGLVA +E +NPF++CD+VT+TT
Sbjct: 309 KILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFDHCDIVTSTT 368

Query: 243 HKSLRGPRAGMIFYRKGPKPPKKGQ-----PEDAVYDFEDKINFSVFPALQGGPHNHQIG 302
           HK LRGPR G+IFYR+GPK  K+G           YD E+KINF+VFP+LQGGPHN+ I 
Sbjct: 369 HKGLRGPRGGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSLQGGPHNNHIA 428

Query: 303 ALAVALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTG 362
           ALA+ALKQ  +P +KAY +Q+K NA AL   L+ +  +LVTGGT+NHL+LWDL P+GLTG
Sbjct: 429 ALAIALKQVATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLLWDLTPMGLTG 488

Query: 363 NKVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVT 422
              EK+C++C+IT+NK A+FGD+  ++PGGVRIG PAMT+RG +E DFE +A+FL +A  
Sbjct: 489 KVYEKVCEMCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFETMADFLIKAAQ 548

Query: 423 ITLNVQKEFGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLM 465
           IT  +Q+E GK  K+F K L  NK+I EL+  VE F+  ++MP  L+
Sbjct: 549 ITSALQREHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMPASLI 595

BLAST of HG10010041 vs. ExPASy Swiss-Prot
Match: Q9LM59 (Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 2.3e-177
Identity = 296/466 (63.52%), Postives = 368/466 (78.97%), Query Frame = 0

Query: 4   VSSWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGM 63
           V +WGN  +   DPEI + +EKEK+RQ RGIELIASENF   AV+EALGS LTNKYSEGM
Sbjct: 134 VRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHLTNKYSEGM 193

Query: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHDR 123
           PG RYY GN++ID+IE LC+ RAL AF ++  KWGVNVQPYS + ANFA +T LL P +R
Sbjct: 194 PGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVFTGLLMPGER 253

Query: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDFRPK 183
           IMGLD PSGGH++HGYYT GGKK+S  SI+FES PYKVD  TGYIDYDKLEEKALD+RPK
Sbjct: 254 IMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLEEKALDYRPK 313

Query: 184 LIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243
           ++ICGGS+YPRDW++ RFR IADKCGA+L+ DMA ISGLVAA+E+ NPF+YCD+VT+TTH
Sbjct: 314 ILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDYCDIVTSTTH 373

Query: 244 KSLRGPRAGMIFYRKGPKPPKKG-----QPEDAVYDFEDKINFSVFPALQGGPHNHQIGA 303
           KSLRGPR G+IFY++G KP K+         +  YDFE+KINFSVFP+LQGGPHN+ I A
Sbjct: 374 KSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSLQGGPHNNHIAA 433

Query: 304 LAVALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGN 363
           LA+ALKQA SP +K Y +QVK NA AL + L+++  KL+TGGT+NHL+LWDL PLGLTG 
Sbjct: 434 LAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWDLTPLGLTGK 493

Query: 364 KVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTI 423
             EK+C++C+ITVNK A+F ++  ++PGGVRIG+PAMTSRG +E +FE +A+FL+RA  I
Sbjct: 494 VYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMADFLYRAAQI 553

Query: 424 TLNVQKEFGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLM 465
               Q+E GKL K+  K + + KEI +L+  VE F+  F MP F M
Sbjct: 554 ASAAQREHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFDM 599

BLAST of HG10010041 vs. ExPASy Swiss-Prot
Match: Q54Z26 (Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=shmt1 PE=1 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 4.9e-175
Identity = 304/461 (65.94%), Postives = 367/461 (79.61%), Query Frame = 0

Query: 1   MDSVSSWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M SVS  GNTPL  VD EIF+L+ +EK RQ +G+ELIASENFTS AV+EALGS  TNKY+
Sbjct: 1   MGSVS--GNTPLKEVDNEIFELMNREKDRQFKGLELIASENFTSRAVMEALGSHFTNKYA 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EG PG+RYYGG E +DE+E LC+ RAL+AF +D +KWGVNVQPYSGSPANFA YTALL+P
Sbjct: 61  EGYPGSRYYGGTEVVDELETLCQKRALKAFRLDESKWGVNVQPYSGSPANFAVYTALLRP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGY T   KKISA+SI+FES+PY++    G IDY +LEE AL F
Sbjct: 121 HDRIMGLDLPSGGHLTHGYQTD-KKKISASSIFFESMPYQI-GADGLIDYQRLEENALLF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           +PKLII G SAYPR+WDY R RAIADK GA L+CDMAH SGLVAAQ   +PF+YCDVVT+
Sbjct: 181 KPKLIISGASAYPREWDYKRMRAIADKVGAYLMCDMAHYSGLVAAQLLDSPFDYCDVVTS 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHK+LRGPR+G+IF+R+G +    G+ E   YD E KINF+VFP+LQGGPH + I  +A
Sbjct: 241 TTHKTLRGPRSGIIFFRRGKRVDGNGK-EIEEYDIESKINFAVFPSLQGGPHENVIAGVA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALK+A S  FK YA QVK NA A+GN LMNKGYKLVT GT+NHL+LWDLRP  LTGNK 
Sbjct: 301 VALKEADSQEFKEYALQVKKNAAAIGNALMNKGYKLVTNGTDNHLILWDLRPKELTGNKF 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EK  D+ NITVNKNAV GD++A++PGG+RIG+ A+TSRGL E DFE+IA+FL R V+I+L
Sbjct: 361 EKAADIANITVNKNAVHGDTNAISPGGIRIGSSALTSRGLKEADFEKIADFLDRIVSISL 420

Query: 421 NVQKEFGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPG 462
            +Q   GK L DF   +  +KE+ +L+ +VE+FS  F +PG
Sbjct: 421 EIQGRVGKKLVDFVVEINKSKELLDLRKEVEEFSSKFTLPG 456

BLAST of HG10010041 vs. ExPASy TrEMBL
Match: A0A0A0LKC8 (Serine hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G145880 PE=3 SV=1)

HSP 1 Score: 955.3 bits (2468), Expect = 9.6e-275
Identity = 461/471 (97.88%), Postives = 467/471 (99.15%), Query Frame = 0

Query: 1   MDSVSSWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MD V+ WGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRAL+AFH DPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDS TGYIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYD+EDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAM+PGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMAPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEFGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           NVQKE+GKLLKDFNKGLVNNKEIE+LKADVEKFSGSFDMPGFLMSEMKYKD
Sbjct: 421 NVQKEYGKLLKDFNKGLVNNKEIEKLKADVEKFSGSFDMPGFLMSEMKYKD 471

BLAST of HG10010041 vs. ExPASy TrEMBL
Match: A0A5A7SVK0 (Serine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold275G00690 PE=3 SV=1)

HSP 1 Score: 954.9 bits (2467), Expect = 1.3e-274
Identity = 461/471 (97.88%), Postives = 466/471 (98.94%), Query Frame = 0

Query: 1   MDSVSSWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MD V+ WGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRAL+AF  DP KWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFRCDPTKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYD+EDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEFGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           N+QKE+GKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD
Sbjct: 421 NIQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 471

BLAST of HG10010041 vs. ExPASy TrEMBL
Match: A0A1S3C536 (Serine hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=6F11 PE=3 SV=1)

HSP 1 Score: 954.9 bits (2467), Expect = 1.3e-274
Identity = 461/471 (97.88%), Postives = 466/471 (98.94%), Query Frame = 0

Query: 1   MDSVSSWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MD V+ WGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRAL+AF  DP KWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFRCDPTKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYD+EDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEFGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           N+QKE+GKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD
Sbjct: 421 NIQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 471

BLAST of HG10010041 vs. ExPASy TrEMBL
Match: A0A6J1CDF6 (Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111009716 PE=3 SV=1)

HSP 1 Score: 949.1 bits (2452), Expect = 6.9e-273
Identity = 458/471 (97.24%), Postives = 464/471 (98.51%), Query Frame = 0

Query: 1   MDSVSSWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+ V++WGNTPL  VDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MEPVNAWGNTPLKAVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRALEAFH DPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHADPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDS TGYIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEFGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           N+QKE+GKLLKDFNKGLVNNK+IE LKADVEKFSGSFDMPGF MSEMKYKD
Sbjct: 421 NIQKEYGKLLKDFNKGLVNNKDIEGLKADVEKFSGSFDMPGFAMSEMKYKD 471

BLAST of HG10010041 vs. ExPASy TrEMBL
Match: A0A6J1H4Y7 (Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111460192 PE=3 SV=1)

HSP 1 Score: 946.8 bits (2446), Expect = 3.4e-272
Identity = 459/471 (97.45%), Postives = 464/471 (98.51%), Query Frame = 0

Query: 1   MDSVSSWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MD V++WGNTPL  VDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDS TG+IDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPE+AVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFK YAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEFGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           NVQKE GKLLKDFNKGLVNNKEIE LKADVEKFSGSF+MPGFLMSEMKYKD
Sbjct: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 471

BLAST of HG10010041 vs. TAIR 10
Match: AT4G13930.1 (serine hydroxymethyltransferase 4 )

HSP 1 Score: 901.4 bits (2328), Expect = 3.2e-262
Identity = 433/471 (91.93%), Postives = 455/471 (96.60%), Query Frame = 0

Query: 1   MDSVSSWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+ VSSWGNT L +VDPEI DLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MEPVSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EG+PGNRYYGGNEFIDEIENLCRSRALEAFH DPA WGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGIPGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKV+ TTGYIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKL+ICGGSAYPRDWDYARFRAIADK GALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPE AVYDFEDKINF+VFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEGAVYDFEDKINFAVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQA +PGFK YAKQVKANAVALGNYLM+KGY++VT GTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLC+IT+NKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQI EFL RAVT+TL
Sbjct: 361 EKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTLTL 420

Query: 421 NVQKEFGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           ++QK +GKLLKDFNKGLVNNK++++LKADVEKFS S++MPGFLMSEMKYKD
Sbjct: 421 DIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPGFLMSEMKYKD 471

BLAST of HG10010041 vs. TAIR 10
Match: AT4G13890.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 816.6 bits (2108), Expect = 1.0e-236
Identity = 384/468 (82.05%), Postives = 430/468 (91.88%), Query Frame = 0

Query: 1   MDSVSSWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+ V SWGNT L+ VDPEI+DLIEKEK RQCRGIELIA+ENFTS AV+EALGS LTNKYS
Sbjct: 1   MEPVYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGG EFIDEIE+LCRSR+LEAFH +P KWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGH+THGYY+SGGK ISATSIYFE+LPYKVDS TGYIDYDKLEEKA+DF
Sbjct: 121 HDRIMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGG++YPR+WDYARFRA+ADK GA LLCDMAH S LVAAQEAA+PFEYCDVVTT
Sbjct: 181 RPKLIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           +THKSLRGPRAGMIFYRKGPKP KKGQPE  VYDF+ KIN +VFPALQ GPHN++IGALA
Sbjct: 241 STHKSLRGPRAGMIFYRKGPKPAKKGQPEGEVYDFDAKINSAVFPALQSGPHNNKIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQ M+P FK YAKQVKANA  L +YL+NKGY LVT GT+NHL+LWDLRPLGLTGNKV
Sbjct: 301 VALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EK+C+LC IT+N+NAVFGD+S LAPGGVRIG PAMTSRGLVEKDFE+I EFLHRAVTITL
Sbjct: 361 EKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTITL 420

Query: 421 NVQKEFGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMK 469
           ++Q+++GK++KDFNKGLVNNKEI+E+KADVE+F+  FDMPGF +SE +
Sbjct: 421 DIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPGFFISESR 468

BLAST of HG10010041 vs. TAIR 10
Match: AT1G36370.1 (serine hydroxymethyltransferase 7 )

HSP 1 Score: 629.8 bits (1623), Expect = 1.8e-180
Identity = 295/467 (63.17%), Postives = 371/467 (79.44%), Query Frame = 0

Query: 3   SVSSWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEG 62
           +V +WG+ P++  DP+I +L+EKEK+RQ RGIELIASENF   AV+EALGS LTNKYSEG
Sbjct: 129 AVRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSHLTNKYSEG 188

Query: 63  MPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHD 122
           MPG RYY GN++ID+IENLC  RAL AF ++  KWGVNVQPYS + ANFA YT LL P +
Sbjct: 189 MPGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAVYTGLLLPGE 248

Query: 123 RIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDFRP 182
           RIMGLD PSGGH++HGY T GGKKISA SI+FES PYKV+  TGYIDYDKLE+KALD+RP
Sbjct: 249 RIMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKLEDKALDYRP 308

Query: 183 KLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTT 242
           K++ICGGS+YPRDWD+AR R IADKCGA+L+CDMAHISGLVA +E +NPF++CD+VT+TT
Sbjct: 309 KILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFDHCDIVTSTT 368

Query: 243 HKSLRGPRAGMIFYRKGPKPPKKGQ-----PEDAVYDFEDKINFSVFPALQGGPHNHQIG 302
           HK LRGPR G+IFYR+GPK  K+G           YD E+KINF+VFP+LQGGPHN+ I 
Sbjct: 369 HKGLRGPRGGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSLQGGPHNNHIA 428

Query: 303 ALAVALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTG 362
           ALA+ALKQ  +P +KAY +Q+K NA AL   L+ +  +LVTGGT+NHL+LWDL P+GLTG
Sbjct: 429 ALAIALKQVATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLLWDLTPMGLTG 488

Query: 363 NKVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVT 422
              EK+C++C+IT+NK A+FGD+  ++PGGVRIG PAMT+RG +E DFE +A+FL +A  
Sbjct: 489 KVYEKVCEMCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFETMADFLIKAAQ 548

Query: 423 ITLNVQKEFGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLM 465
           IT  +Q+E GK  K+F K L  NK+I EL+  VE F+  ++MP  L+
Sbjct: 549 ITSALQREHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMPASLI 595

BLAST of HG10010041 vs. TAIR 10
Match: AT1G22020.1 (serine hydroxymethyltransferase 6 )

HSP 1 Score: 623.2 bits (1606), Expect = 1.7e-178
Identity = 296/466 (63.52%), Postives = 368/466 (78.97%), Query Frame = 0

Query: 4   VSSWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGM 63
           V +WGN  +   DPEI + +EKEK+RQ RGIELIASENF   AV+EALGS LTNKYSEGM
Sbjct: 134 VRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHLTNKYSEGM 193

Query: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHDR 123
           PG RYY GN++ID+IE LC+ RAL AF ++  KWGVNVQPYS + ANFA +T LL P +R
Sbjct: 194 PGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVFTGLLMPGER 253

Query: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDFRPK 183
           IMGLD PSGGH++HGYYT GGKK+S  SI+FES PYKVD  TGYIDYDKLEEKALD+RPK
Sbjct: 254 IMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLEEKALDYRPK 313

Query: 184 LIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243
           ++ICGGS+YPRDW++ RFR IADKCGA+L+ DMA ISGLVAA+E+ NPF+YCD+VT+TTH
Sbjct: 314 ILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDYCDIVTSTTH 373

Query: 244 KSLRGPRAGMIFYRKGPKPPKKG-----QPEDAVYDFEDKINFSVFPALQGGPHNHQIGA 303
           KSLRGPR G+IFY++G KP K+         +  YDFE+KINFSVFP+LQGGPHN+ I A
Sbjct: 374 KSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSLQGGPHNNHIAA 433

Query: 304 LAVALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGN 363
           LA+ALKQA SP +K Y +QVK NA AL + L+++  KL+TGGT+NHL+LWDL PLGLTG 
Sbjct: 434 LAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWDLTPLGLTGK 493

Query: 364 KVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTI 423
             EK+C++C+ITVNK A+F ++  ++PGGVRIG+PAMTSRG +E +FE +A+FL+RA  I
Sbjct: 494 VYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMADFLYRAAQI 553

Query: 424 TLNVQKEFGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLM 465
               Q+E GKL K+  K + + KEI +L+  VE F+  F MP F M
Sbjct: 554 ASAAQREHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFDM 599

BLAST of HG10010041 vs. TAIR 10
Match: AT4G37930.1 (serine transhydroxymethyltransferase 1 )

HSP 1 Score: 562.0 bits (1447), Expect = 4.5e-160
Identity = 279/468 (59.62%), Postives = 351/468 (75.00%), Query Frame = 0

Query: 9   NTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRY 68
           N PL  VDPEI D+IE EK RQ +G+ELI SENFTS +V++A+GS +TNKYSEG PG RY
Sbjct: 52  NAPLEEVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARY 111

Query: 69  YGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHDRIMGLD 128
           YGGNE+ID  E LC+ RALEAF +DP KWGVNVQP SGSPANF  YTALL+PH+RIM LD
Sbjct: 112 YGGNEYIDMAETLCQKRALEAFRLDPEKWGVNVQPLSGSPANFHVYTALLKPHERIMALD 171

Query: 129 LPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDFRPKLIICG 188
           LP GGHL+HGY T   KKISA SI+FE++PY++D +TGYIDYD++E+ A  FRPKLI+ G
Sbjct: 172 LPHGGHLSHGYQTD-TKKISAVSIFFETMPYRLDESTGYIDYDQMEKSATLFRPKLIVAG 231

Query: 189 GSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTHKSLRG 248
            SAY R +DYAR R + +K  A++L DMAHISGLVAA    +PF+Y DVVTTTTHKSLRG
Sbjct: 232 ASAYARLYDYARIRKVCNKQKAVMLADMAHISGLVAANVIPSPFDYADVVTTTTHKSLRG 291

Query: 249 PRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALAVALKQAMS 308
           PR  MIF+RKG K   K Q ++ +YDFEDKIN +VFP LQGGPHNH I  LAVALKQA +
Sbjct: 292 PRGAMIFFRKGVKEINK-QGKEVLYDFEDKINQAVFPGLQGGPHNHTITGLAVALKQATT 351

Query: 309 PGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCN 368
             +KAY +QV +N+      LM +GY+LV+GGT+NHLVL +L+P G+ G++VEK+ +  +
Sbjct: 352 SEYKAYQEQVLSNSAKFAQTLMERGYELVSGGTDNHLVLVNLKPKGIDGSRVEKVLEAVH 411

Query: 369 ITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITLNVQKEF-G 428
           I  NKN V GD SA+ PGG+R+G PA+TSRG VE+DF ++AE+  +AVTI L V+ E  G
Sbjct: 412 IASNKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEYFDKAVTIALKVKSEAQG 471

Query: 429 KLLKDFNKGLVNN----KEIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
             LKDF   + ++     EI +L+ +VE+F+  F   GF    MKYK+
Sbjct: 472 TKLKDFVSAMESSSTIQSEIAKLRHEVEEFAKQFPTIGFEKETMKYKN 517

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038875387.14.7e-27698.73serine hydroxymethyltransferase 4 [Benincasa hispida][more]
XP_004138967.12.0e-27497.88serine hydroxymethyltransferase 4 [Cucumis sativus] >KGN61489.1 hypothetical pro... [more]
XP_008457222.12.6e-27497.88PREDICTED: serine hydroxymethyltransferase 4 [Cucumis melo] >KAA0033337.1 serine... [more]
XP_022138598.11.4e-27297.24serine hydroxymethyltransferase 4 [Momordica charantia][more]
XP_022959093.17.0e-27297.45serine hydroxymethyltransferase 4-like [Cucurbita moschata] >KAG6574849.1 Serine... [more]
Match NameE-valueIdentityDescription
O232544.4e-26191.93Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 S... [more]
Q9SVM41.4e-23582.05Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 S... [more]
Q84WV02.5e-17963.17Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 S... [more]
Q9LM592.3e-17763.52Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 S... [more]
Q54Z264.9e-17565.94Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=shmt1 ... [more]
Match NameE-valueIdentityDescription
A0A0A0LKC89.6e-27597.88Serine hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G145880 PE=3 ... [more]
A0A5A7SVK01.3e-27497.88Serine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3C5361.3e-27497.88Serine hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=6F11 PE=3 SV=1[more]
A0A6J1CDF66.9e-27397.24Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111009716 P... [more]
A0A6J1H4Y73.4e-27297.45Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111460192 PE... [more]
Match NameE-valueIdentityDescription
AT4G13930.13.2e-26291.93serine hydroxymethyltransferase 4 [more]
AT4G13890.11.0e-23682.05Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT1G36370.11.8e-18063.17serine hydroxymethyltransferase 7 [more]
AT1G22020.11.7e-17863.52serine hydroxymethyltransferase 6 [more]
AT4G37930.14.5e-16059.62serine transhydroxymethyltransferase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 41..316
e-value: 2.7E-215
score: 716.8
IPR001085Serine hydroxymethyltransferasePIRSFPIRSF000412SHMTcoord: 1..466
e-value: 3.6E-189
score: 626.6
IPR001085Serine hydroxymethyltransferasePANTHERPTHR11680SERINE HYDROXYMETHYLTRANSFERASEcoord: 1..470
IPR001085Serine hydroxymethyltransferaseHAMAPMF_00051SHMTcoord: 11..461
score: 42.853245
IPR001085Serine hydroxymethyltransferaseCDDcd00378SHMTcoord: 15..453
e-value: 0.0
score: 621.529
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 16..460
e-value: 2.7E-215
score: 716.8
IPR039429Serine hydroxymethyltransferase-like domainPFAMPF00464SHMTcoord: 12..411
e-value: 2.4E-192
score: 638.9
NoneNo IPR availablePANTHERPTHR11680:SF33SERINE HYDROXYMETHYLTRANSFERASEcoord: 1..470
IPR019798Serine hydroxymethyltransferase, pyridoxal phosphate binding sitePROSITEPS00096SHMTcoord: 236..252
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 6..463

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10010041.1HG10010041.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046655 folic acid metabolic process
biological_process GO:0019264 glycine biosynthetic process from serine
biological_process GO:0006565 L-serine catabolic process
biological_process GO:0032259 methylation
biological_process GO:0035999 tetrahydrofolate interconversion
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004372 glycine hydroxymethyltransferase activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0070905 serine binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003824 catalytic activity