HG10010035 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10010035
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Chaperone protein dnaJ 20
Location	Chr06: 17344475 .. 17345866 (-)
RNA-Seq Expression	HG10010035
Synteny	HG10010035

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGTAGCTGCGGTTTGGCTTTATCAGGCACCGATTCTCGTTACTATTTACCGGCGATGCCTCCGATTTTCTCTCCAAGAAGAGCCTCTCCGCTAGATGGAACTTCGCAGGTTCTGTTCCCTAAATCGGCACGGTCTCCTTCTGCTTGCATCAAAGTAAAGGCCGCTGCCGTCAACGACGCGGTTGTCTCCGTCGCCGCCGAGATGAGCTTCTACGAGCTTTTAGGAGTACCGGAGTTGGGGTCGATGGTGGAGATTAAGCAGGCCTACAAGCAGATGGCTCTGAAATATCATCCTGACGTTTCGCCGCCGGACCGGATCGAGGAGTATACGAGGAAGTTCATCAAGGTTCAGGAGGCGTACGAGACGTTATCCGATCCTAATATGAGAGCTTTGTATGATAGAGACATGGCGAGAGGTGTTCACCTTGCTTTTTCTGCTCGAAGACGCAGCTACAACCATGAGGTAGCTTTCTTGGCTATTTTTTAGCTCAAAATTGGTTGCGGAATCAATTAGGATGTTTTCTCTACCTTATTTTATTGCAATTTATTTTGTTATATTGAATTGATTTTGACCTGTTTTGAACAGTTTCGCCTCCTGATCATGAATTCTTGTTAATTTCTTTGACCTATTCGTTTCTTCAACCTGGATTACTCGCAAGCACGCAGTCTTGGTTTTCATTTGTTTCTGAATTATTGCTTTGTATTCCTCATTTGCCTACGCGTTCTGCTTCTACATGAATCGGCTTAGGAATTGAGCTGCAAAAATCCCTTGATTTGTTTTCTCATCCATTTACAGAAAGTATATCTTCTTGCTACTTTTAATTGAACTGAATTTACAAACTACAAAGGTCGATTTTCAATATGCAAATACTTTTGAGATTCTGTTTTTTCAAACAATAGATTAACCATAATTTTTTACTCTTATGATTCTTCTATTTTCTCTCTTTCTTAAGATTGCAGCATGCCTCTTAAATAATTTGCAACATGAAGAAATCATACAAGAGAAATAACCCTTTGAATGAGATTTCATGGCTTTTACTTTTTCATTTCACCATCCATTACTAAAAATTAAATCTAATCCTAATTCAAATGACTTATGTGAATCAAAACATGTATAATCTGGACCAAATCGTGACTTCTGCATGTAACTCATATGACAGATATTGGTTCTTTTAGTTCATATATCCAAATTTCTTATATTAAATCTGAACTTATCTTCTTTCCCTGCATTACTCAGGAAATGGAAGAGAAAAGTGGGTGGAAGAACCGTTGGCAGGATCAGTTATCAGGATTGAAGAGAAGAAGCATGAACAAAGATTCAGGAGCAAACATGTCATGGGGAGCTCGGATGCGCAGACAAAGGGACGAGCTTTATGACGACGATGTATAA

mRNA sequence

ATGCGTAGCTGCGGTTTGGCTTTATCAGGCACCGATTCTCGTTACTATTTACCGGCGATGCCTCCGATTTTCTCTCCAAGAAGAGCCTCTCCGCTAGATGGAACTTCGCAGGTTCTGTTCCCTAAATCGGCACGGTCTCCTTCTGCTTGCATCAAAGTAAAGGCCGCTGCCGTCAACGACGCGGTTGTCTCCGTCGCCGCCGAGATGAGCTTCTACGAGCTTTTAGGAGTACCGGAGTTGGGGTCGATGGTGGAGATTAAGCAGGCCTACAAGCAGATGGCTCTGAAATATCATCCTGACGTTTCGCCGCCGGACCGGATCGAGGAGTATACGAGGAAGTTCATCAAGGTTCAGGAGGCGTACGAGACGTTATCCGATCCTAATATGAGAGCTTTGTATGATAGAGACATGGCGAGAGGTGTTCACCTTGCTTTTTCTGCTCGAAGACGCAGCTACAACCATGAGGAAATGGAAGAGAAAAGTGGGTGGAAGAACCGTTGGCAGGATCAGTTATCAGGATTGAAGAGAAGAAGCATGAACAAAGATTCAGGAGCAAACATGTCATGGGGAGCTCGGATGCGCAGACAAAGGGACGAGCTTTATGACGACGATGTATAA

Coding sequence (CDS)

ATGCGTAGCTGCGGTTTGGCTTTATCAGGCACCGATTCTCGTTACTATTTACCGGCGATGCCTCCGATTTTCTCTCCAAGAAGAGCCTCTCCGCTAGATGGAACTTCGCAGGTTCTGTTCCCTAAATCGGCACGGTCTCCTTCTGCTTGCATCAAAGTAAAGGCCGCTGCCGTCAACGACGCGGTTGTCTCCGTCGCCGCCGAGATGAGCTTCTACGAGCTTTTAGGAGTACCGGAGTTGGGGTCGATGGTGGAGATTAAGCAGGCCTACAAGCAGATGGCTCTGAAATATCATCCTGACGTTTCGCCGCCGGACCGGATCGAGGAGTATACGAGGAAGTTCATCAAGGTTCAGGAGGCGTACGAGACGTTATCCGATCCTAATATGAGAGCTTTGTATGATAGAGACATGGCGAGAGGTGTTCACCTTGCTTTTTCTGCTCGAAGACGCAGCTACAACCATGAGGAAATGGAAGAGAAAAGTGGGTGGAAGAACCGTTGGCAGGATCAGTTATCAGGATTGAAGAGAAGAAGCATGAACAAAGATTCAGGAGCAAACATGTCATGGGGAGCTCGGATGCGCAGACAAAGGGACGAGCTTTATGACGACGATGTATAA

Protein sequence

MRSCGLALSGTDSRYYLPAMPPIFSPRRASPLDGTSQVLFPKSARSPSACIKVKAAAVNDAVVSVAAEMSFYELLGVPELGSMVEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPNMRALYDRDMARGVHLAFSARRRSYNHEEMEEKSGWKNRWQDQLSGLKRRSMNKDSGANMSWGARMRRQRDELYDDDV

Homology

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BLAST of HG10010035 vs. NCBI nr
Match: XP_038875872.1 (chaperone protein dnaJ 20, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 352.4 bits (903), Expect = 2.6e-93
Identity = 177/204 (86.76%), Postives = 189/204 (92.65%), Query Frame = 0


Query: 1   MRSCGLALSGTDSRYYLPAMPPIFSPRRASPLDGTSQVLFPKSARSPSACIKVKAAAVND 60
           MRSCGLALSGTDSR YLPAMPP FSPRRASP DGTSQVLFPKSAR PSAC++VKA  VN+
Sbjct: 1   MRSCGLALSGTDSRCYLPAMPPTFSPRRASPPDGTSQVLFPKSARHPSACVRVKATVVNE 60

Query: 61  AVVSVAAEMSFYELLGVPELGSMVEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEA 120
           AVVS   EMSFYE+LGVPE GSMVEIKQAYKQMALKYHPDVSP DRIEEYTRKFIKVQEA
Sbjct: 61  AVVSDDVEMSFYEVLGVPESGSMVEIKQAYKQMALKYHPDVSPRDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPNMRALYDRDMARGVHLAFSARRRSYNHEEMEEKSGWKNRWQDQLSGLKRRSMN 180
           YETLSDP+MRALYD DMA G+HLAFSARRR YNHEEMEEKSGWKNRW+DQLSGLK+RSMN
Sbjct: 121 YETLSDPSMRALYDSDMAIGLHLAFSARRRGYNHEEMEEKSGWKNRWKDQLSGLKKRSMN 180

Query: 181 KDSGANMSWGARMRRQRDELYDDD 205
           KDS A+MSWGARMRRQRD+L++DD
Sbjct: 181 KDSRASMSWGARMRRQRDKLHEDD 204

[Close]

BLAST of HG10010035 vs. NCBI nr
Match: XP_023549024.1 (chaperone protein dnaJ 20, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 345.9 bits (886), Expect = 2.4e-91
Identity = 174/204 (85.29%), Postives = 183/204 (89.71%), Query Frame = 0


Query: 1   MRSCGLALSGTDSRYYLPAMPPIFSPRRASPLDGTSQVLFPKSARSPSACIKVKAAAVND 60
           MRSCGLALSGTDSR YLPAMPPIFSP RA P +G SQVLFPK  R PSAC++ KA  VN+
Sbjct: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE 60

Query: 61  AVVSVAAEMSFYELLGVPELGSMVEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEA 120
           A+VS A E+SFYELLGVPE GSMVEIKQAYKQMA KYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPNMRALYDRDMARGVHLAFSARRRSYNHEEMEEKSGWKNRWQDQLSGLKRRSMN 180
           YETLSDP MR LYDRDMARG+HLAFSARRR YNHEEMEEK GWKNRWQDQLS LKRRSMN
Sbjct: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMEEKGGWKNRWQDQLSELKRRSMN 180

Query: 181 KDSGANMSWGARMRRQRDELYDDD 205
           K+S ANMSWGARMRRQRDEL+DDD
Sbjct: 181 KNSRANMSWGARMRRQRDELHDDD 204

[Close]

BLAST of HG10010035 vs. NCBI nr
Match: XP_023006292.1 (chaperone protein dnaJ 20, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 341.3 bits (874), Expect = 5.9e-90
Identity = 172/204 (84.31%), Postives = 181/204 (88.73%), Query Frame = 0


Query: 1   MRSCGLALSGTDSRYYLPAMPPIFSPRRASPLDGTSQVLFPKSARSPSACIKVKAAAVND 60
           MRSCGLALSGTDSR YLPAMPPIFSP RA PLDG SQVLFPK  R PSAC++ KA  V +
Sbjct: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPLDGNSQVLFPKLTRPPSACVRAKATVVKE 60

Query: 61  AVVSVAAEMSFYELLGVPELGSMVEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEA 120
            +VS A E+SFYELLGVPE GSMVEIKQAYKQMA KYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  TLVSDAIEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPNMRALYDRDMARGVHLAFSARRRSYNHEEMEEKSGWKNRWQDQLSGLKRRSMN 180
           YETLSDP MR LYDRDMARG+HLAFSARRR YNHEEMEEK GWK+RWQDQLS LKRRSMN
Sbjct: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMEEKGGWKSRWQDQLSELKRRSMN 180

Query: 181 KDSGANMSWGARMRRQRDELYDDD 205
           K+S ANMSWGARMRRQ DEL+DDD
Sbjct: 181 KNSRANMSWGARMRRQGDELHDDD 204

[Close]

BLAST of HG10010035 vs. NCBI nr
Match: XP_022959157.1 (chaperone protein dnaJ 20, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 340.9 bits (873), Expect = 7.7e-90
Identity = 171/204 (83.82%), Postives = 183/204 (89.71%), Query Frame = 0


Query: 1   MRSCGLALSGTDSRYYLPAMPPIFSPRRASPLDGTSQVLFPKSARSPSACIKVKAAAVND 60
           MRSCGLALSGTDSR YLPAMPPIFSP RA P +G SQVLFPK  R PSAC++ KA  VN+
Sbjct: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE 60

Query: 61  AVVSVAAEMSFYELLGVPELGSMVEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEA 120
           A+VS A E+SFYELLGVPE GSMVEIKQAYKQMA KYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPNMRALYDRDMARGVHLAFSARRRSYNHEEMEEKSGWKNRWQDQLSGLKRRSMN 180
           YETLSDP MR LYDRDMARG+HLAFSARRR YNHEEM+EK GWK+RWQDQLS LKRRSMN
Sbjct: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMDEKGGWKSRWQDQLSELKRRSMN 180

Query: 181 KDSGANMSWGARMRRQRDELYDDD 205
           K+S ANMSWGARMRRQRDEL++DD
Sbjct: 181 KNSRANMSWGARMRRQRDELHNDD 204

[Close]

BLAST of HG10010035 vs. NCBI nr
Match: KAG6574846.1 (Chaperone protein dnaJ 20, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013422.1 Chaperone protein dnaJ 20, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 337.0 bits (863), Expect = 1.1e-88
Identity = 169/204 (82.84%), Postives = 182/204 (89.22%), Query Frame = 0


Query: 1   MRSCGLALSGTDSRYYLPAMPPIFSPRRASPLDGTSQVLFPKSARSPSACIKVKAAAVND 60
           MRSCGLALSGTDSR YLPAMPPIFSP RA P +G SQVLFPK  R PSAC++ KA  VN+
Sbjct: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE 60

Query: 61  AVVSVAAEMSFYELLGVPELGSMVEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEA 120
           A+VS A E+SFYELLGVPE GSMVEIKQAYKQMA KYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPNMRALYDRDMARGVHLAFSARRRSYNHEEMEEKSGWKNRWQDQLSGLKRRSMN 180
           YETLSDP MR LYDRDMARG+HLAFSARRR YNHEEM+EK GWK+RWQDQLS LKRRSMN
Sbjct: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMDEKGGWKSRWQDQLSELKRRSMN 180

Query: 181 KDSGANMSWGARMRRQRDELYDDD 205
           K+S ANMSWGARMR QRDEL++D+
Sbjct: 181 KNSRANMSWGARMRSQRDELHNDE 204

[Close]

BLAST of HG10010035 vs. ExPASy Swiss-Prot
Match: Q9SDN0 (Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ20 PE=1 SV=2)

HSP 1 Score: 171.8 bits (434), Expect = 8.1e-42
Identity = 96/183 (52.46%), Postives = 126/183 (68.85%), Query Frame = 0


Query: 21  PPIFSPRRASPLDGTS---QVLFPKSARSPSACIKVKAAAVNDAVVSVAAEMSFYELLGV 80
           P  +  +  S L  TS    + +P   R  S   ++++   +D  V  + ++SFY+LLGV
Sbjct: 16  PFFYKQQPISSLQPTSIPTTISYPTRTRFSST--RIQSRLTHDDPVKQSEDLSFYDLLGV 75

Query: 81  PELGSMVEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPNMRALYDRDM 140
            E  ++ EIKQAYKQ+A KYHPDVSPPDR+EEYT +FI+VQEAYETLSDP  R LYDRD+
Sbjct: 76  TESVTLPEIKQAYKQLARKYHPDVSPPDRVEEYTDRFIRVQEAYETLSDPRRRVLYDRDL 135

Query: 141 ARGVHLAFSARRRS-YNHEEMEEKSGWKNRWQDQLSGLKRRSMNKDSGANMSWGARMRRQ 200
           + G   +FS RR++ Y+ E +EEKS WK +WQ QLSGL+RRS  KD+   MSW ARMRRQ
Sbjct: 136 SMGFSFSFSGRRQNRYDQEVVEEKSEWKAKWQTQLSGLRRRSNQKDNN-TMSWAARMRRQ 195

[Close]

BLAST of HG10010035 vs. ExPASy Swiss-Prot
Match: Q6RSN5 (Chaperone protein DnaJ OS=Rhizobium radiobacter OX=358 GN=dnaJ PE=1 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 6.7e-12
Identity = 35/69 (50.72%), Postives = 46/69 (66.67%), Query Frame = 0


Query: 67  AEMSFYELLGVPELGSMVEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEAYETLSD 126
           A+  FYE LGV +     E+K A++++A+KYHPD +P D   E  RKF ++ EAYETL D
Sbjct: 2   AKADFYETLGVSKTADEKELKSAFRKLAMKYHPDKNPDDADSE--RKFKEINEAYETLKD 61

Query: 127 PNMRALYDR 136
           P  RA YDR
Sbjct: 62  PQKRAAYDR 68

[Close]

BLAST of HG10010035 vs. ExPASy Swiss-Prot
Match: P53863 (J protein JJJ1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=JJJ1 PE=1 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 6.7e-12
Identity = 35/64 (54.69%), Postives = 47/64 (73.44%), Query Frame = 0


Query: 71  FYELLGVPELGSMVEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPNMR 130
           +YELLGV    S +E+K+AY++ AL+YHPD + PD +EE T+KF  ++ AYE LSDP  R
Sbjct: 5   YYELLGVETHASDLELKKAYRKKALQYHPDKN-PDNVEEATQKFAVIRAAYEVLSDPQER 64

Query: 131 ALYD 135
           A YD
Sbjct: 65  AWYD 67

[Close]

BLAST of HG10010035 vs. ExPASy Swiss-Prot
Match: B7IFE0 (Chaperone protein DnaJ OS=Thermosipho africanus (strain TCF52B) OX=484019 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 8.7e-12
Identity = 36/69 (52.17%), Postives = 48/69 (69.57%), Query Frame = 0


Query: 67  AEMSFYELLGVPELGSMVEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEAYETLSD 126
           A+  +YE+LGV    S  EI+QAYKQ+  K+HPD +  +R +E   KF ++QEAYE LSD
Sbjct: 2   AKKDYYEILGVSRNASQEEIRQAYKQLIKKWHPDRNHQNR-KEAEEKFKEIQEAYEVLSD 61

Query: 127 PNMRALYDR 136
           P  RA+YDR
Sbjct: 62  PEKRAMYDR 69

[Close]

BLAST of HG10010035 vs. ExPASy Swiss-Prot
Match: A1TLH8 (Chaperone protein DnaJ OS=Acidovorax citrulli (strain AAC00-1) OX=397945 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.9e-11
Identity = 35/75 (46.67%), Postives = 50/75 (66.67%), Query Frame = 0


Query: 67  AEMSFYELLGVPELGSMVEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEAYETLSD 126
           ++  FYE+LGVP+  S  EIK+AY+++A+K+HPD +  D  +    KF + +EAYE LSD
Sbjct: 2   SKRDFYEVLGVPKNASDEEIKKAYRKLAMKHHPDRNQGDAAKPAEEKFKEAKEAYEMLSD 61

Query: 127 PNMRALYDRDMARGV 142
           P  RA YD+    GV
Sbjct: 62  PQKRAAYDQFGHAGV 76

[Close]

BLAST of HG10010035 vs. ExPASy TrEMBL
Match: A0A6J1KVG4 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111499073 PE=4 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 2.9e-90
Identity = 172/204 (84.31%), Postives = 181/204 (88.73%), Query Frame = 0


Query: 1   MRSCGLALSGTDSRYYLPAMPPIFSPRRASPLDGTSQVLFPKSARSPSACIKVKAAAVND 60
           MRSCGLALSGTDSR YLPAMPPIFSP RA PLDG SQVLFPK  R PSAC++ KA  V +
Sbjct: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPLDGNSQVLFPKLTRPPSACVRAKATVVKE 60

Query: 61  AVVSVAAEMSFYELLGVPELGSMVEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEA 120
            +VS A E+SFYELLGVPE GSMVEIKQAYKQMA KYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  TLVSDAIEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPNMRALYDRDMARGVHLAFSARRRSYNHEEMEEKSGWKNRWQDQLSGLKRRSMN 180
           YETLSDP MR LYDRDMARG+HLAFSARRR YNHEEMEEK GWK+RWQDQLS LKRRSMN
Sbjct: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMEEKGGWKSRWQDQLSELKRRSMN 180

Query: 181 KDSGANMSWGARMRRQRDELYDDD 205
           K+S ANMSWGARMRRQ DEL+DDD
Sbjct: 181 KNSRANMSWGARMRRQGDELHDDD 204

[Close]

BLAST of HG10010035 vs. ExPASy TrEMBL
Match: A0A6J1H3S2 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111460232 PE=4 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 3.7e-90
Identity = 171/204 (83.82%), Postives = 183/204 (89.71%), Query Frame = 0


Query: 1   MRSCGLALSGTDSRYYLPAMPPIFSPRRASPLDGTSQVLFPKSARSPSACIKVKAAAVND 60
           MRSCGLALSGTDSR YLPAMPPIFSP RA P +G SQVLFPK  R PSAC++ KA  VN+
Sbjct: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE 60

Query: 61  AVVSVAAEMSFYELLGVPELGSMVEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEA 120
           A+VS A E+SFYELLGVPE GSMVEIKQAYKQMA KYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPNMRALYDRDMARGVHLAFSARRRSYNHEEMEEKSGWKNRWQDQLSGLKRRSMN 180
           YETLSDP MR LYDRDMARG+HLAFSARRR YNHEEM+EK GWK+RWQDQLS LKRRSMN
Sbjct: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMDEKGGWKSRWQDQLSELKRRSMN 180

Query: 181 KDSGANMSWGARMRRQRDELYDDD 205
           K+S ANMSWGARMRRQRDEL++DD
Sbjct: 181 KNSRANMSWGARMRRQRDELHNDD 204

[Close]

BLAST of HG10010035 vs. ExPASy TrEMBL
Match: A0A5A7SVL1 (Chaperone protein dnaJ 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold275G00560 PE=4 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 3.3e-86
Identity = 166/204 (81.37%), Postives = 182/204 (89.22%), Query Frame = 0


Query: 1   MRSCGLALSGTDSRYYLPAMPPIFSPRRASPLDGTSQVLFPKSARSPSACIKVKAAAVND 60
           MRSCGLALSGTDSR Y PA PPIFSPRRAS     SQV F KS R PSAC++VKAA +ND
Sbjct: 1   MRSCGLALSGTDSRCYFPATPPIFSPRRAS----NSQVSFLKSPRPPSACVRVKAAFIND 60

Query: 61  AVVSVAAEMSFYELLGVPELGSMVEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEA 120
           AVVS A E+SFYELLGVPE GSM+EIKQAYKQMALKYHPDVSPP RIEEYTRKFIK+QEA
Sbjct: 61  AVVSDAVEVSFYELLGVPESGSMLEIKQAYKQMALKYHPDVSPPGRIEEYTRKFIKLQEA 120

Query: 121 YETLSDPNMRALYDRDMARGVHLAFSARRRSYNHEEMEEKSGWKNRWQDQLSGLKRRSMN 180
           YETLSDPNMRALYDRD+ARG+  AFS+R+R Y+HEEMEEKSGW+NRWQDQLSGLKRRSMN
Sbjct: 121 YETLSDPNMRALYDRDLARGLQFAFSSRKRCYDHEEMEEKSGWRNRWQDQLSGLKRRSMN 180

Query: 181 KDSGANMSWGARMRRQRDELYDDD 205
           +DS ANMSWGARMRR+RDEL+ +D
Sbjct: 181 RDSKANMSWGARMRRERDELHKED 200

[Close]

BLAST of HG10010035 vs. ExPASy TrEMBL
Match: A0A1S3C500 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103496947 PE=4 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 3.3e-86
Identity = 166/204 (81.37%), Postives = 182/204 (89.22%), Query Frame = 0


Query: 1   MRSCGLALSGTDSRYYLPAMPPIFSPRRASPLDGTSQVLFPKSARSPSACIKVKAAAVND 60
           MRSCGLALSGTDSR Y PA PPIFSPRRAS     SQV F KS R PSAC++VKAA +ND
Sbjct: 1   MRSCGLALSGTDSRCYFPATPPIFSPRRAS----NSQVSFLKSPRPPSACVRVKAAFIND 60

Query: 61  AVVSVAAEMSFYELLGVPELGSMVEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEA 120
           AVVS A E+SFYELLGVPE GSM+EIKQAYKQMALKYHPDVSPP RIEEYTRKFIK+QEA
Sbjct: 61  AVVSDAVEVSFYELLGVPESGSMLEIKQAYKQMALKYHPDVSPPGRIEEYTRKFIKLQEA 120

Query: 121 YETLSDPNMRALYDRDMARGVHLAFSARRRSYNHEEMEEKSGWKNRWQDQLSGLKRRSMN 180
           YETLSDPNMRALYDRD+ARG+  AFS+R+R Y+HEEMEEKSGW+NRWQDQLSGLKRRSMN
Sbjct: 121 YETLSDPNMRALYDRDLARGLQFAFSSRKRCYDHEEMEEKSGWRNRWQDQLSGLKRRSMN 180

Query: 181 KDSGANMSWGARMRRQRDELYDDD 205
           +DS ANMSWGARMRR+RDEL+ +D
Sbjct: 181 RDSKANMSWGARMRRERDELHKED 200

[Close]

BLAST of HG10010035 vs. ExPASy TrEMBL
Match: A0A6J1KFW2 (chaperone protein dnaJ 20, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111494708 PE=4 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 5.6e-86
Identity = 166/204 (81.37%), Postives = 182/204 (89.22%), Query Frame = 0


Query: 1   MRSCGLALSGTDSRYYLPAMPPIFSPRRASPLDGTSQVLFPKSARSPSACIKVKAAAVND 60
           MR  GLALSGTDSR ++PAMPPIFSPRRASP  G SQVLFPKS R+PS C++ KAA VN+
Sbjct: 1   MRGYGLALSGTDSRCFVPAMPPIFSPRRASPSVGNSQVLFPKSTRNPSLCVRAKAAVVNE 60

Query: 61  AVVSVAAEMSFYELLGVPELGSMVEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEA 120
           A+VS A E+SFY+LLG+PE GSM EIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  ALVSDAVEISFYDLLGIPESGSMAEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPNMRALYDRDMARGVHLAFSARRRSYNHEEMEEKSGWKNRWQDQLSGLKRRSMN 180
           YETLSDP MRALYDRDM  G+HLAFSARRR  N+EEME KSGWK+ WQDQLSGL+RRSMN
Sbjct: 121 YETLSDPRMRALYDRDMLGGLHLAFSARRRG-NNEEMEVKSGWKSLWQDQLSGLRRRSMN 180

Query: 181 KDSGANMSWGARMRRQRDELYDDD 205
           KDSGA+MSWGARMRRQRDEL DD+
Sbjct: 181 KDSGASMSWGARMRRQRDELNDDE 203

[Close]

BLAST of HG10010035 vs. TAIR 10
Match: AT4G13830.2 (DNAJ-like 20 )

HSP 1 Score: 171.8 bits (434), Expect = 5.8e-43
Identity = 96/183 (52.46%), Postives = 126/183 (68.85%), Query Frame = 0


Query: 21  PPIFSPRRASPLDGTS---QVLFPKSARSPSACIKVKAAAVNDAVVSVAAEMSFYELLGV 80
           P  +  +  S L  TS    + +P   R  S   ++++   +D  V  + ++SFY+LLGV
Sbjct: 16  PFFYKQQPISSLQPTSIPTTISYPTRTRFSST--RIQSRLTHDDPVKQSEDLSFYDLLGV 75

Query: 81  PELGSMVEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPNMRALYDRDM 140
            E  ++ EIKQAYKQ+A KYHPDVSPPDR+EEYT +FI+VQEAYETLSDP  R LYDRD+
Sbjct: 76  TESVTLPEIKQAYKQLARKYHPDVSPPDRVEEYTDRFIRVQEAYETLSDPRRRVLYDRDL 135

Query: 141 ARGVHLAFSARRRS-YNHEEMEEKSGWKNRWQDQLSGLKRRSMNKDSGANMSWGARMRRQ 200
           + G   +FS RR++ Y+ E +EEKS WK +WQ QLSGL+RRS  KD+   MSW ARMRRQ
Sbjct: 136 SMGFSFSFSGRRQNRYDQEVVEEKSEWKAKWQTQLSGLRRRSNQKDNN-TMSWAARMRRQ 195

[Close]

BLAST of HG10010035 vs. TAIR 10
Match: AT4G13830.1 (DNAJ-like 20 )

HSP 1 Score: 123.6 bits (309), Expect = 1.8e-28
Identity = 66/134 (49.25%), Postives = 90/134 (67.16%), Query Frame = 0


Query: 21  PPIFSPRRASPLDGTS---QVLFPKSARSPSACIKVKAAAVNDAVVSVAAEMSFYELLGV 80
           P  +  +  S L  TS    + +P   R  S   ++++   +D  V  + ++SFY+LLGV
Sbjct: 16  PFFYKQQPISSLQPTSIPTTISYPTRTRFSST--RIQSRLTHDDPVKQSEDLSFYDLLGV 75

Query: 81  PELGSMVEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPNMRALYDRDM 140
            E  ++ EIKQAYKQ+A KYHPDVSPPDR+EEYT +FI+VQEAYETLSDP  R LYDRD+
Sbjct: 76  TESVTLPEIKQAYKQLARKYHPDVSPPDRVEEYTDRFIRVQEAYETLSDPRRRVLYDRDL 135

Query: 141 ARGVHLAFSARRRS 152
           + G   +FS RR++
Sbjct: 136 SMGFSFSFSGRRQN 147

[Close]

BLAST of HG10010035 vs. TAIR 10
Match: AT3G62600.1 (DNAJ heat shock family protein )

HSP 1 Score: 65.5 bits (158), Expect = 5.8e-11
Identity = 33/73 (45.21%), Postives = 50/73 (68.49%), Query Frame = 0


Query: 63  VSVAAEMSFYELLGVPELGSMVEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEAYE 122
           + V A  S+Y++L VP+  S  +IK+AY+++ALKYHPD +  +  EE TRKF ++  AYE
Sbjct: 19  ICVLAGKSYYDVLQVPKGASDEQIKRAYRKLALKYHPDKNQGN--EEATRKFAEINNAYE 78

Query: 123 TLSDPNMRALYDR 136
            LSD   R +Y++
Sbjct: 79  VLSDEEKREIYNK 89

[Close]

BLAST of HG10010035 vs. TAIR 10
Match: AT1G59980.1 (ARG1-like 2 )

HSP 1 Score: 63.9 bits (154), Expect = 1.7e-10
Identity = 32/63 (50.79%), Postives = 41/63 (65.08%), Query Frame = 0


Query: 72  YELLGVPELGSMVEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPNMRA 131
           YE+LG+P   +  EIK AY++MAL+YHPD +P D +      F +V  AYE LSDP  R 
Sbjct: 25  YEVLGIPSNSTDQEIKSAYRRMALRYHPDKNPDDPVA--AEMFKEVTFAYEVLSDPENRR 84

Query: 132 LYD 135
           LYD
Sbjct: 85  LYD 85

[Close]

BLAST of HG10010035 vs. TAIR 10
Match: AT4G39960.1 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 63.5 bits (153), Expect = 2.2e-10
Identity = 34/78 (43.59%), Postives = 47/78 (60.26%), Query Frame = 0


Query: 64  SVAAEMSFYELLGVPELGSMVEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEAYET 123
           +V A+  FY +LGV +  +  EIK AY+++A  YHPDV+     E+   KF ++  AYE 
Sbjct: 79  TVRADTDFYSVLGVSKNATKAEIKSAYRKLARSYHPDVNKDAGAED---KFKEISNAYEI 138

Query: 124 LSDPNMRALYDRDMARGV 142
           LSD   R+LYDR    GV
Sbjct: 139 LSDDEKRSLYDRYGEAGV 153

The following BLAST results are available for this feature:

BLAST of HG10010035 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038875872.1	2.6e-93	86.76	chaperone protein dnaJ 20, chloroplastic-like [Benincasa hispida]	[more]
XP_023549024.1	2.4e-91	85.29	chaperone protein dnaJ 20, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
XP_023006292.1	5.9e-90	84.31	chaperone protein dnaJ 20, chloroplastic-like [Cucurbita maxima]	[more]
XP_022959157.1	7.7e-90	83.82	chaperone protein dnaJ 20, chloroplastic-like [Cucurbita moschata]	[more]
KAG6574846.1	1.1e-88	82.84	Chaperone protein dnaJ 20, chloroplastic, partial [Cucurbita argyrosperma subsp....	[more]

BLAST of HG10010035 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SDN0	8.1e-42	52.46	Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ2...	[more]
Q6RSN5	6.7e-12	50.72	Chaperone protein DnaJ OS=Rhizobium radiobacter OX=358 GN=dnaJ PE=1 SV=1	[more]
P53863	6.7e-12	54.69	J protein JJJ1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=55929...	[more]
B7IFE0	8.7e-12	52.17	Chaperone protein DnaJ OS=Thermosipho africanus (strain TCF52B) OX=484019 GN=dna...	[more]
A1TLH8	1.9e-11	46.67	Chaperone protein DnaJ OS=Acidovorax citrulli (strain AAC00-1) OX=397945 GN=dnaJ...	[more]

BLAST of HG10010035 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KVG4	2.9e-90	84.31	chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1H3S2	3.7e-90	83.82	chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A5A7SVL1	3.3e-86	81.37	Chaperone protein dnaJ 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3C500	3.3e-86	81.37	chaperone protein dnaJ 20, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A6J1KFW2	5.6e-86	81.37	chaperone protein dnaJ 20, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC11149...	[more]

BLAST of HG10010035 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G13830.2	5.8e-43	52.46	DNAJ-like 20	[more]
AT4G13830.1	1.8e-28	49.25	DNAJ-like 20	[more]
AT3G62600.1	5.8e-11	45.21	DNAJ heat shock family protein	[more]
AT1G59980.1	1.7e-10	50.79	ARG1-like 2	[more]
AT4G39960.1	2.2e-10	43.59	Molecular chaperone Hsp40/DnaJ family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 109..129 score: 41.57 coord: 72..90 score: 46.77 coord: 90..105 score: 46.65
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 69..129 e-value: 2.6E-21 score: 86.8
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 71..134 e-value: 1.0E-20 score: 73.6
IPR001623	DnaJ domain	PROSITE	PS50076	DNAJ_2	coord: 70..137 score: 19.201035
IPR001623	DnaJ domain	CDD	cd06257	DnaJ	coord: 71..126 e-value: 3.25191E-19 score: 75.6597
IPR036869	Chaperone J-domain superfamily	GENE3D	1.10.287.110	DnaJ domain	coord: 56..172 e-value: 2.5E-22 score: 81.0
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	46565	Chaperone J-domain	coord: 65..141
None	No IPR available	PANTHER	PTHR45090:SF4	CHAPERONE PROTEIN DNAJ 20, CHLOROPLASTIC	coord: 44..200
None	No IPR available	PANTHER	PTHR45090	CHAPERONE PROTEIN DNAJ 20 CHLOROPLASTIC	coord: 44..200
IPR018253	DnaJ domain, conserved site	PROSITE	PS00636	DNAJ_1	coord: 114..133

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10010035.1	HG10010035.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0061077	chaperone-mediated protein folding
cellular_component	GO:0009507	chloroplast