HG10009882 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10009882
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionNitrate reductase
LocationChr06: 12657684 .. 12662936 (+)
RNA-Seq ExpressionHG10009882
SyntenyHG10009882
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCTGCTGTTAATCACAAAACCCTCGACTTGGAACCGGTCTCCAATAATGCCAATTTTCCGGTTCAACGCTGCAACTCATTCCGGTCCAGTAGCTTCAAATTCAAGCGCCAAGCCGAAAAACCGCTCATTATTGACGATGACGATTCCTCCTTTGATCGTGACGATGAGAACAGTAATTACTTCCGGGAATTGATCCAGAAGGGGAAAGAGGAAATCGAGCCGTCCATTCTTGATCCGAGGGATGAGGGGACGGGGGATAGTGGGATCATGAGAAATCCTTGTATGGTCCGACTTACCGGAAAACACCCTTTCAACTCTGAGCCGCCGTTGGACCGTCTCATGCGCCACGGGTTCATTACTCCGGCCCCACTTCACTATGTTCGTAACCATGGTCCGGTTCCCAAGACTGATTGGTCCAGCTGGACGATTGACGTCACTGGACTCGTTGAAAACCCCACATCCTTCACTCTTGACCAACTTGTCAATGACTTCAGGTCCTTAAATAGCCATTAAACTCAAAACCCTCAAAACTTGTTTCTAAATGATTTTCTTTTTTCTAATTATTTTTATAAAAATTATTTATTTTGTCTTTTCTATTTTTCTACTTTGCAAACTAAAAAAAAAAAAATACATAGTTTTTAAAAAGTTGATTTTTTATTTTTAGAATTCGAATAAAAATGCAATACTCTCAAAAACAATGTTAGAAAATTGGGATCAAGAAAACAAAAATTAACTTTAAAAAATTATGATGGCTTTTTCCTCAGCATTTAATTTTCAGTTTTTAATTTTTGTACATTAATCTCATTTCACCTTTAAATTTCTTCTTTTTGTTATCTACTTTCTATTAATATTTTCAAAAACTAAGTCAAATTTTTAAAATTAAAAAATATAGTTTTTAAAAATTTATTTTCTAAAATTTAATTACTAATTCAGGTTCATAAACCAAATGGGTTACCAAACCTTAATTTCCAAATTTTAAACTTACAAATCATCTTGGTTTCTTTTCATTTTCTTTAATAAGGAATTTTCTTAAGATTTTTGAGCCACTTTTAAATGTATCATATCAAAAAGTTAGCAGGAGCTTCAACTAACTTGTGTGTCAGCAATTCATTTCCTTCTTTAGGATTTTTTTTTCTACTTTTTTTTGTCTTTTTAGTTATTAGAATTAGGTTTACATTTTGAAAACGTTTTGTTAATAATAATACAAAAGAAAGTAGAGTTATGACAAAATATGACTAGAACAAAATCTTCATAAGCTTATTTTATTTTTTTAGAAATACGAGGTTTAAAAAATAGAACAAAACCCCACAATTGATTATTAATATATATATGAAATTGAAATAAAATGAAATTACAAACATATTCAACAACTTAAATATGTGGTATATATCTATTTCTAACTCTTTATTCATATTCTTAACACAGATCTCTGGAGTTTCCGGTGACCATCATGTGCTCCGGCAACCGACGGAAGGAGCAAAACATGGTTAAACAAACCATCGGATTCCATTGGGGAGCCGCCGTGACCTCCACCTCACTGTGGCGAGGAGTGCCATTACGCGAAGTACTGAAACGGTGCGGAATTTTCAGCCGTCAAAGAGGAGGCTTATACATATGGATGGAAGGCGCCGACGATCTCCCCGGCGGCGGCGGCTGCAGCTACGGCACCAGCATAAGAAGAGAAATGGCAATGGACCCAGCAAGAGACATCATAATAGCCTACAAGCAAAACGGCGACCCTCTCCCGCCGGACCACGGATTTCCGGTTAGGATGATCGTTCCGGGGTTCACCGGCGGCCGCATGGTCAAGTGGCTCAAACGAATCATCGTCACCAATAGAGAATCCGACAATTTTTATCATTTTATGGACAACCGTGTGCTTCCATCCCACGTGGCCGATTCCGAGCTGGCACATGCTGAAGGTAATACATTTATTTATTTTGATTAAAAATTGAATTAAAATTTAGTTTTAAACTTCCAGTGTCTAAATATTTAATAAATTACTAAGAGCTCGTTTCATAAATCATTTATTTTTGGTTTCTCATCTTTAAAAATTAAGCTTATAAAAATATTTTTCACTATAAGTTTATTTGTTTTGATTTTCATTTTTTATCATTATTTTCAAAAACATATTTTGTGTTTAGAATTTGACTGCGAATTGAAGTCTTTCTTATAAGAAATATTAAAAAAAATCACAATTTTTAGAAGGAAATGAATATAAATTTCAAAATAGAAAACTAAAAACGAGATTGATATTGAATGTAGCTTCAAATTTTAATTTTATATCTACTAAAATTTTCAAACCTCTAATTTTGTATTTAACAAGTTACTAATCTAATTGACATTTTTTAAAATTCATCTATTTATTGGATATAAAATTAAAAGTTTGATGATTTATTAGATATATGAAATTAAATTTTTTGTATGATAGATTCATTCTTTTAAAAAAGAATGTTGAATATGTCATAAACAAATTGGTTACAAAAACTAAAAGTTACTCCACATTCTTAAAATTTAAAAACCTATTAGGTATAATTTTAGTAAAAATGGATTATTCATAAAAAATAAAAATAAAAACAATTAAAATGATATAAGTGGGTGATGTTATTTAGGTTGGTGGTACAAACCGGAGTATGTAATCTACGAGATGAACATAAACTCCGTGATAACGACGCCGTCACACGACGAGACACTGCCGATCAATTCACAAACGACGCAATCCACGTACACGTTGAGGGGGTACGCATATTCCGGTGAGCCCTAATAATAATTTTCCACGTATTTTCGTAGTTATTTTCCACGTGAGATTAATTTTATTAAATGATTTATTTTTTTCCGTCCAGGTGGTGGGAAGAAAGTGACACGTGTGCAGGTGACGTTGGACGGTGGAGAGACGTGGAAAATATGTAATTTGGAATACCATGAAAAACCAAACAAGTATGGGAAATATTGGTGCTGGATTTTCTGGTCAGTGGAGGTGGAAATTGTGGACCTGTTTGGCTCCAAGGAGATTGCTGTCCGAGCATGGGATGAGACCAACAACACACAACCAGAAAACCTCAATTGGAATCTCATGGTAATTAAGTTTCTATTTCTACATTAAAATAATTATAATTAATTAACTCCTTATCGTCACAAAATATCTTACAAAATCTATGGCCTATACACATATGCAACTAAAATAATAAAAATTTAACTAAATCATAAGAAACCTGACATATATCACGTCTCTTTCTTCCATTATTATAATATCTTCAACTATCTATTTTTTTTTTTTTAAATAGAAATATTAGCACTATTTTACTTCCTCCCACCTTTTAGATTGACTTTTCTATTTCTTCCCCTCTGATCCATATGATTTACCTAAGAAAAAAAAATTGCTTATAAAATAATCATTAATTAAACTGCAATATGTTTAGCGAGACTCAATCATTTGAAAAATTAAACAAAACATAGGGTTTTTACAATAAAAGTAAATGCATACTGAATAATAATAAATTGATAATATTTTTGAAAAATGACATAAAAGGTAAGAATGAGTACATTATCATGTAATTAAAAATTTGTAAAAGTGAACACGAAGTGAAGAACTGGTAAATTTGAGGGAAAAAATCAGACACAAATGAATGCCCTTTTGTATAGCGATAATAATAATATTAATTATATAGTTTATTTCAAACAATACTCTATCAATAGTACCAAAATATTATTTATATTTGTGATGTACGACTTCAACGTTATCATACAAAAAATATAAAATCTCTCTTAAATAATATAGTAATGTGTAATGTCCTCAATATATATATATATCAATTATAGTCTCTCGACGTTAGTTTTCATCCAAATTGATTATATTATAATAGTACTAATTTTCAGGCAAGAAATAGAAAAATTAGTTTTAGTGGAAAGACTCGTAGATAATAAAAGTAGCCAAGTAGGGGACTAAAATTAAATATTTAAAAGTTAAAAAATATTTGGATGCAGGGCATGATGAACAACTCATGGTACCGAATCAAGATAAACATATGCAAACCCCAAGAAGGAGAGATCGGTATCGTGTTCCGCCACCCAACTCTTGCCGGCAACCAATCCGGCGGCTGGAAGGAAAAGGGCAAACCCATTCCGACCAACTCCTCTTCCCAACCCTCAAACAATAAGACAACCACCGCGGCCACCACCACCACCTTCCCCATGTCGGAAGTCAAAACCCACAACTCGCCGGAATCCGCCTGGATCGTCGTCCACAACCAAGTATACGACTGCACTCCTTTCCTCAAAGCCCACCCAGGCGGCGCCGACAGCATCCTCATCAACGCCGGCACCGACTGCACTGAAGAGTTCGACGCCATCCACTCCGAGAAAGCCATAAAAATGCTCGAAGACTACAAAATCGGTGACTTATTGATCCTAAACGAAAACAAAGACGCGACGTCATCACCCAAATTAATTAGTGGACACGTGGCACTGGTCCCACGTGAGAAAATTCCCGTGAAATTGATATCGAAGATTTCAATTTCTCACAACACGAGAATTTTCAGATTTGGTCTTTCTTCAGAAGATCAAACTTTAGGACTACCCGTTGGAAAGCACATATTTTTATGCGCTAATATCGGCGGGAAGCTCTGTATGAGAGCCTACACGCCGTCCAGCGCCGTTGACCAAATGGGTTACTTTGACTTGGTCGTAAAAATTTACTTCAAGAACGTCCATCCTGATTTCCCCAACGGCGGCCTCATGTCACAGTATCTAGACTCGCTTCCATTGGGCTCAATTGTGGACGTAAAAGGCCCATTGGGCCACATCGAGTACATGGGCCGCGGCAACTTCACGGTCCACGGAAAGCCCAAATTCGCGAAGAAGCTTGCCATGGTGGCAGGTGGGACCGGGATAACTCCTATCTATCAAGTGGTGCAGGCGATTTTGAAAGATCCGGAGGACGAGACGGAGATGTTTGTTGTGTATGCGAATCGGACGGAGGAGGATATTTTGCTGAGGGAAGAGTTGGACACGTGGGCGAAGGAGAATGAGAGATTGAAGATTTGGTACGTGGTGAAGGAGTGTGTTAAAGAAGGGTGGCCGTACAGTGTAGGGTATATTAAGGAGAGTGTTTTGAGAGAGCATATTCCGATGGGGTCGCCGGAGACTTTGGCTTTGGTGTGTGGGCCGCCGCCCATGATTGAGCTCACAGTTAAGCCCATATTGAAGGATCTGGAGTATGATTTGGAGAGTTCGTTGCTGGTGTTTTGA

mRNA sequence

ATGGCGGCTGCTGTTAATCACAAAACCCTCGACTTGGAACCGGTCTCCAATAATGCCAATTTTCCGGTTCAACGCTGCAACTCATTCCGGTCCAGTAGCTTCAAATTCAAGCGCCAAGCCGAAAAACCGCTCATTATTGACGATGACGATTCCTCCTTTGATCGTGACGATGAGAACAGTAATTACTTCCGGGAATTGATCCAGAAGGGGAAAGAGGAAATCGAGCCGTCCATTCTTGATCCGAGGGATGAGGGGACGGGGGATAGTGGGATCATGAGAAATCCTTGTATGGTCCGACTTACCGGAAAACACCCTTTCAACTCTGAGCCGCCGTTGGACCGTCTCATGCGCCACGGGTTCATTACTCCGGCCCCACTTCACTATGTTCGTAACCATGGTCCGGTTCCCAAGACTGATTGGTCCAGCTGGACGATTGACGTCACTGGACTCGTTGAAAACCCCACATCCTTCACTCTTGACCAACTTGTCAATGACTTCAGATCTCTGGAGTTTCCGGTGACCATCATGTGCTCCGGCAACCGACGGAAGGAGCAAAACATGGTTAAACAAACCATCGGATTCCATTGGGGAGCCGCCGTGACCTCCACCTCACTGTGGCGAGGAGTGCCATTACGCGAAGTACTGAAACGGTGCGGAATTTTCAGCCGTCAAAGAGGAGGCTTATACATATGGATGGAAGGCGCCGACGATCTCCCCGGCGGCGGCGGCTGCAGCTACGGCACCAGCATAAGAAGAGAAATGGCAATGGACCCAGCAAGAGACATCATAATAGCCTACAAGCAAAACGGCGACCCTCTCCCGCCGGACCACGGATTTCCGGTTAGGATGATCGTTCCGGGGTTCACCGGCGGCCGCATGGTCAAGTGGCTCAAACGAATCATCGTCACCAATAGAGAATCCGACAATTTTTATCATTTTATGGACAACCGTGTGCTTCCATCCCACGTGGCCGATTCCGAGCTGGCACATGCTGAAGGTTGGTGGTACAAACCGGAGTATGTAATCTACGAGATGAACATAAACTCCGTGATAACGACGCCGTCACACGACGAGACACTGCCGATCAATTCACAAACGACGCAATCCACGTACACGTTGAGGGGGTACGCATATTCCGGTGGTGGGAAGAAAGTGACACGTGTGCAGGTGACGTTGGACGGTGGAGAGACGTGGAAAATATGTAATTTGGAATACCATGAAAAACCAAACAAGTATGGGAAATATTGGTGCTGGATTTTCTGGTCAGTGGAGGTGGAAATTGTGGACCTGTTTGGCTCCAAGGAGATTGCTGTCCGAGCATGGGATGAGACCAACAACACACAACCAGAAAACCTCAATTGGAATCTCATGGGCATGATGAACAACTCATGGTACCGAATCAAGATAAACATATGCAAACCCCAAGAAGGAGAGATCGGTATCGTGTTCCGCCACCCAACTCTTGCCGGCAACCAATCCGGCGGCTGGAAGGAAAAGGGCAAACCCATTCCGACCAACTCCTCTTCCCAACCCTCAAACAATAAGACAACCACCGCGGCCACCACCACCACCTTCCCCATGTCGGAAGTCAAAACCCACAACTCGCCGGAATCCGCCTGGATCGTCGTCCACAACCAAGTATACGACTGCACTCCTTTCCTCAAAGCCCACCCAGGCGGCGCCGACAGCATCCTCATCAACGCCGGCACCGACTGCACTGAAGAGTTCGACGCCATCCACTCCGAGAAAGCCATAAAAATGCTCGAAGACTACAAAATCGGTGACTTATTGATCCTAAACGAAAACAAAGACGCGACGTCATCACCCAAATTAATTAGTGGACACGTGGCACTGGTCCCACGTGAGAAAATTCCCGTGAAATTGATATCGAAGATTTCAATTTCTCACAACACGAGAATTTTCAGATTTGGTCTTTCTTCAGAAGATCAAACTTTAGGACTACCCGTTGGAAAGCACATATTTTTATGCGCTAATATCGGCGGGAAGCTCTGTATGAGAGCCTACACGCCGTCCAGCGCCGTTGACCAAATGGGTTACTTTGACTTGGTCGTAAAAATTTACTTCAAGAACGTCCATCCTGATTTCCCCAACGGCGGCCTCATGTCACAGTATCTAGACTCGCTTCCATTGGGCTCAATTGTGGACGTAAAAGGCCCATTGGGCCACATCGAGTACATGGGCCGCGGCAACTTCACGGTCCACGGAAAGCCCAAATTCGCGAAGAAGCTTGCCATGGTGGCAGGTGGGACCGGGATAACTCCTATCTATCAAGTGGTGCAGGCGATTTTGAAAGATCCGGAGGACGAGACGGAGATGTTTGTTGTGTATGCGAATCGGACGGAGGAGGATATTTTGCTGAGGGAAGAGTTGGACACGTGGGCGAAGGAGAATGAGAGATTGAAGATTTGGTACGTGGTGAAGGAGTGTGTTAAAGAAGGGTGGCCGTACAGTGTAGGGTATATTAAGGAGAGTGTTTTGAGAGAGCATATTCCGATGGGGTCGCCGGAGACTTTGGCTTTGGTGTGTGGGCCGCCGCCCATGATTGAGCTCACAGTTAAGCCCATATTGAAGGATCTGGAGTATGATTTGGAGAGTTCGTTGCTGGTGTTTTGA

Coding sequence (CDS)

ATGGCGGCTGCTGTTAATCACAAAACCCTCGACTTGGAACCGGTCTCCAATAATGCCAATTTTCCGGTTCAACGCTGCAACTCATTCCGGTCCAGTAGCTTCAAATTCAAGCGCCAAGCCGAAAAACCGCTCATTATTGACGATGACGATTCCTCCTTTGATCGTGACGATGAGAACAGTAATTACTTCCGGGAATTGATCCAGAAGGGGAAAGAGGAAATCGAGCCGTCCATTCTTGATCCGAGGGATGAGGGGACGGGGGATAGTGGGATCATGAGAAATCCTTGTATGGTCCGACTTACCGGAAAACACCCTTTCAACTCTGAGCCGCCGTTGGACCGTCTCATGCGCCACGGGTTCATTACTCCGGCCCCACTTCACTATGTTCGTAACCATGGTCCGGTTCCCAAGACTGATTGGTCCAGCTGGACGATTGACGTCACTGGACTCGTTGAAAACCCCACATCCTTCACTCTTGACCAACTTGTCAATGACTTCAGATCTCTGGAGTTTCCGGTGACCATCATGTGCTCCGGCAACCGACGGAAGGAGCAAAACATGGTTAAACAAACCATCGGATTCCATTGGGGAGCCGCCGTGACCTCCACCTCACTGTGGCGAGGAGTGCCATTACGCGAAGTACTGAAACGGTGCGGAATTTTCAGCCGTCAAAGAGGAGGCTTATACATATGGATGGAAGGCGCCGACGATCTCCCCGGCGGCGGCGGCTGCAGCTACGGCACCAGCATAAGAAGAGAAATGGCAATGGACCCAGCAAGAGACATCATAATAGCCTACAAGCAAAACGGCGACCCTCTCCCGCCGGACCACGGATTTCCGGTTAGGATGATCGTTCCGGGGTTCACCGGCGGCCGCATGGTCAAGTGGCTCAAACGAATCATCGTCACCAATAGAGAATCCGACAATTTTTATCATTTTATGGACAACCGTGTGCTTCCATCCCACGTGGCCGATTCCGAGCTGGCACATGCTGAAGGTTGGTGGTACAAACCGGAGTATGTAATCTACGAGATGAACATAAACTCCGTGATAACGACGCCGTCACACGACGAGACACTGCCGATCAATTCACAAACGACGCAATCCACGTACACGTTGAGGGGGTACGCATATTCCGGTGGTGGGAAGAAAGTGACACGTGTGCAGGTGACGTTGGACGGTGGAGAGACGTGGAAAATATGTAATTTGGAATACCATGAAAAACCAAACAAGTATGGGAAATATTGGTGCTGGATTTTCTGGTCAGTGGAGGTGGAAATTGTGGACCTGTTTGGCTCCAAGGAGATTGCTGTCCGAGCATGGGATGAGACCAACAACACACAACCAGAAAACCTCAATTGGAATCTCATGGGCATGATGAACAACTCATGGTACCGAATCAAGATAAACATATGCAAACCCCAAGAAGGAGAGATCGGTATCGTGTTCCGCCACCCAACTCTTGCCGGCAACCAATCCGGCGGCTGGAAGGAAAAGGGCAAACCCATTCCGACCAACTCCTCTTCCCAACCCTCAAACAATAAGACAACCACCGCGGCCACCACCACCACCTTCCCCATGTCGGAAGTCAAAACCCACAACTCGCCGGAATCCGCCTGGATCGTCGTCCACAACCAAGTATACGACTGCACTCCTTTCCTCAAAGCCCACCCAGGCGGCGCCGACAGCATCCTCATCAACGCCGGCACCGACTGCACTGAAGAGTTCGACGCCATCCACTCCGAGAAAGCCATAAAAATGCTCGAAGACTACAAAATCGGTGACTTATTGATCCTAAACGAAAACAAAGACGCGACGTCATCACCCAAATTAATTAGTGGACACGTGGCACTGGTCCCACGTGAGAAAATTCCCGTGAAATTGATATCGAAGATTTCAATTTCTCACAACACGAGAATTTTCAGATTTGGTCTTTCTTCAGAAGATCAAACTTTAGGACTACCCGTTGGAAAGCACATATTTTTATGCGCTAATATCGGCGGGAAGCTCTGTATGAGAGCCTACACGCCGTCCAGCGCCGTTGACCAAATGGGTTACTTTGACTTGGTCGTAAAAATTTACTTCAAGAACGTCCATCCTGATTTCCCCAACGGCGGCCTCATGTCACAGTATCTAGACTCGCTTCCATTGGGCTCAATTGTGGACGTAAAAGGCCCATTGGGCCACATCGAGTACATGGGCCGCGGCAACTTCACGGTCCACGGAAAGCCCAAATTCGCGAAGAAGCTTGCCATGGTGGCAGGTGGGACCGGGATAACTCCTATCTATCAAGTGGTGCAGGCGATTTTGAAAGATCCGGAGGACGAGACGGAGATGTTTGTTGTGTATGCGAATCGGACGGAGGAGGATATTTTGCTGAGGGAAGAGTTGGACACGTGGGCGAAGGAGAATGAGAGATTGAAGATTTGGTACGTGGTGAAGGAGTGTGTTAAAGAAGGGTGGCCGTACAGTGTAGGGTATATTAAGGAGAGTGTTTTGAGAGAGCATATTCCGATGGGGTCGCCGGAGACTTTGGCTTTGGTGTGTGGGCCGCCGCCCATGATTGAGCTCACAGTTAAGCCCATATTGAAGGATCTGGAGTATGATTTGGAGAGTTCGTTGCTGGTGTTTTGA

Protein sequence

MAAAVNHKTLDLEPVSNNANFPVQRCNSFRSSSFKFKRQAEKPLIIDDDDSSFDRDDENSNYFRELIQKGKEEIEPSILDPRDEGTGDSGIMRNPCMVRLTGKHPFNSEPPLDRLMRHGFITPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFTLDQLVNDFRSLEFPVTIMCSGNRRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGGLYIWMEGADDLPGGGGCSYGTSIRREMAMDPARDIIIAYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKWLKRIIVTNRESDNFYHFMDNRVLPSHVADSELAHAEGWWYKPEYVIYEMNINSVITTPSHDETLPINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWCWIFWSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQEGEIGIVFRHPTLAGNQSGGWKEKGKPIPTNSSSQPSNNKTTTAATTTTFPMSEVKTHNSPESAWIVVHNQVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSEKAIKMLEDYKIGDLLILNENKDATSSPKLISGHVALVPREKIPVKLISKISISHNTRIFRFGLSSEDQTLGLPVGKHIFLCANIGGKLCMRAYTPSSAVDQMGYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLPLGSIVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEEDILLREELDTWAKENERLKIWYVVKECVKEGWPYSVGYIKESVLREHIPMGSPETLALVCGPPPMIELTVKPILKDLEYDLESSLLVF
Homology
BLAST of HG10009882 vs. NCBI nr
Match: XP_038875756.1 (nitrate reductase [NADH]-like [Benincasa hispida])

HSP 1 Score: 1733.0 bits (4487), Expect = 0.0e+00
Identity = 832/877 (94.87%), Postives = 852/877 (97.15%), Query Frame = 0

Query: 1   MAAAVNHKTLDLEPVSNNANFPVQRCNSFRSSSFKFKRQAEKPLIIDDDDSSFDRDDENS 60
           MAA VN K+LDLEPVS N NFPVQRCNSFRSSSFK KR AEKPLI+DDDDSSFDRDDEN 
Sbjct: 1   MAATVNPKSLDLEPVS-NGNFPVQRCNSFRSSSFKLKRHAEKPLIVDDDDSSFDRDDENI 60

Query: 61  NYFRELIQKGKEEIEPSILDPRDEGTGDSGIMRNPCMVRLTGKHPFNSEPPLDRLMRHGF 120
           +YFR+LI+KGKEEIE SILDPRDEGT DSGIMRNPCMVRLTGKHPFNSEPPL+RLMRHGF
Sbjct: 61  HYFRDLIRKGKEEIEASILDPRDEGTADSGIMRNPCMVRLTGKHPFNSEPPLERLMRHGF 120

Query: 121 ITPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFTLDQLVNDFRSLEFPVTIMCSGN 180
           ITPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFTLDQL+NDFRSLEFPVTIMCSGN
Sbjct: 121 ITPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFTLDQLLNDFRSLEFPVTIMCSGN 180

Query: 181 RRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGGLYIWMEGADDLPG 240
           RRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRG LYIWMEGADDLPG
Sbjct: 181 RRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALYIWMEGADDLPG 240

Query: 241 GGGCSYGTSIRREMAMDPARDIIIAYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKWLKRI 300
           GGGCSYGTSIRREMAMDP+RDIIIAYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKWLKRI
Sbjct: 241 GGGCSYGTSIRREMAMDPSRDIIIAYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKWLKRI 300

Query: 301 IVTNRESDNFYHFMDNRVLPSHVADSELAHAEGWWYKPEYVIYEMNINSVITTPSHDETL 360
           IVTNRESDN+YHFMDNRVLPSHVADSELAHAEGWWYKPEYVIYEMNINSVITTPSHDETL
Sbjct: 301 IVTNRESDNYYHFMDNRVLPSHVADSELAHAEGWWYKPEYVIYEMNINSVITTPSHDETL 360

Query: 361 PINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWCWIF 420
           PINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEY+EKPNKYGKYWCWIF
Sbjct: 361 PINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYYEKPNKYGKYWCWIF 420

Query: 421 WSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQEGEIG 480
           WS+EVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQ+GEIG
Sbjct: 421 WSIEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQKGEIG 480

Query: 481 IVFRHPTLAGNQSGGWKEKGKPIPTNSSSQPSNNKTTTAATTTTFPMSEVKTHNSPESAW 540
           IVFRHPTLAGNQSGGWKEK KPIPTN SSQPSNNKTTT  TTTTF MSEVKTHNS ESAW
Sbjct: 481 IVFRHPTLAGNQSGGWKEKDKPIPTNYSSQPSNNKTTT-TTTTTFSMSEVKTHNSLESAW 540

Query: 541 IVVHNQVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSEKAIKMLEDYKIGDLLILN 600
           IVVHN VYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHS+KAIKML+DYKIGDLLILN
Sbjct: 541 IVVHNHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAIKMLQDYKIGDLLILN 600

Query: 601 ENKDATSSPKLISGHVALVPREKIPVKLISKISISHNTRIFRFGLSSEDQTLGLPVGKHI 660
           +NKD TSSPK I+ HVALVPREKIPVKLISKISISHNTRIFRF LSSEDQTLGLPVGKHI
Sbjct: 601 DNKDDTSSPKPITKHVALVPREKIPVKLISKISISHNTRIFRFSLSSEDQTLGLPVGKHI 660

Query: 661 FLCANIGGKLCMRAYTPSSAVDQMGYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLPLGSI 720
           FLCANI GKLCMRAYTPSSAVDQMGYFDLVVKIYFKN+HPDFPNGGLMSQYLDSLPL SI
Sbjct: 661 FLCANIDGKLCMRAYTPSSAVDQMGYFDLVVKIYFKNIHPDFPNGGLMSQYLDSLPLSSI 720

Query: 721 VDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPEDETEMF 780
           VD+KGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPEDETEMF
Sbjct: 721 VDIKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPEDETEMF 780

Query: 781 VVYANRTEEDILLREELDTWAKENERLKIWYVVKECVKEGWPYSVGYIKESVLREHIPMG 840
           VVYANRTEEDILLREELDTWAKEN+RLKIWYVVKE VKEGWPYSVGYI+E VLREHIPMG
Sbjct: 781 VVYANRTEEDILLREELDTWAKENQRLKIWYVVKEGVKEGWPYSVGYIRECVLREHIPMG 840

Query: 841 SPETLALVCGPPPMIELTVKPILKDLEYDLESSLLVF 878
           SPETLALVCGPPPMIELTVKPILKDLEYDLESSLLVF
Sbjct: 841 SPETLALVCGPPPMIELTVKPILKDLEYDLESSLLVF 875

BLAST of HG10009882 vs. NCBI nr
Match: NP_001267688.1 (nitrate reductase [NAD(P)H]-like [Cucumis sativus] >AFH74423.1 cucumber nitrate reductase 3 [Cucumis sativus] >KGN50981.1 hypothetical protein Csa_008642 [Cucumis sativus])

HSP 1 Score: 1669.1 bits (4321), Expect = 0.0e+00
Identity = 793/878 (90.32%), Postives = 838/878 (95.44%), Query Frame = 0

Query: 1   MAAAVNHKTLDLEPVSNNANFPVQRCNSFRSSSFKFKRQAEKPLIIDDDDSSFDRDDENS 60
           MA AVNHKTLDLEPVS+  NFPVQRCNSFRS+SFKFKRQAEKPL+IDDDDSSFDRD+EN 
Sbjct: 1   MATAVNHKTLDLEPVSSTGNFPVQRCNSFRSNSFKFKRQAEKPLLIDDDDSSFDRDEENI 60

Query: 61  NYFRELIQKGKEEIEPSILDPRDEGTGDSGIMRNPCMVRLTGKHPFNSEPPLDRLMRHGF 120
           +YFR+LI+KGKEEIEPSILDPRD+GT DSGIMRNPCMVRLTGKHPFNSEPPLD LMRHGF
Sbjct: 61  DYFRDLIRKGKEEIEPSILDPRDQGTADSGIMRNPCMVRLTGKHPFNSEPPLDHLMRHGF 120

Query: 121 ITPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFTLDQLVNDFRSLEFPVTIMCSGN 180
           ITPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFTLDQLVN+FRSLEFPVTIMCSGN
Sbjct: 121 ITPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFTLDQLVNNFRSLEFPVTIMCSGN 180

Query: 181 RRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGGLYIWMEGADDLPG 240
           RRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRG LY+WMEGAD+LPG
Sbjct: 181 RRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALYVWMEGADNLPG 240

Query: 241 GGGCSYGTSIRREMAMDPARDIIIAYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKWLKRI 300
           GGGCSYGTSIRREMAMDP+RDIIIAYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKWLKRI
Sbjct: 241 GGGCSYGTSIRREMAMDPSRDIIIAYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKWLKRI 300

Query: 301 IVTNRESDNFYHFMDNRVLPSHVADSELAHAEGWWYKPEYVIYEMNINSVITTPSHDETL 360
           IVTNRESDN+YHFMDNRVLPSHV  SELAHAEGWWYKPEYVIYEMNINSVITTPSH+ETL
Sbjct: 301 IVTNRESDNYYHFMDNRVLPSHVDSSELAHAEGWWYKPEYVIYEMNINSVITTPSHNETL 360

Query: 361 PINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWCWIF 420
            INS+TT STYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWCWIF
Sbjct: 361 LINSETTLSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWCWIF 420

Query: 421 WSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQEGEIG 480
           WSV+VEI+DLFGSK+IAVRAWDET NTQPENLNWNLMGMMNNSWYRIKINICKPQ+GEIG
Sbjct: 421 WSVDVEILDLFGSKQIAVRAWDETTNTQPENLNWNLMGMMNNSWYRIKINICKPQKGEIG 480

Query: 481 IVFRHPTLAGNQSGGWKEKGKPIPTNSS-SQPSNNKTTTAATTTTFPMSEVKTHNSPESA 540
           IVFRHPT+AGN +GGW+EK KP PT+ S SQ  +NK     +TTTF MSEVKTHNS ESA
Sbjct: 481 IVFRHPTVAGNNAGGWQEKDKPNPTDESPSQQKDNK-----STTTFSMSEVKTHNSSESA 540

Query: 541 WIVVHNQVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSEKAIKMLEDYKIGDLLIL 600
           WIVV N VYDCTPFLK HPGGADSILINAGTDCTEEF+AIHS+KAIKMLEDYKIGDLLIL
Sbjct: 541 WIVVQNHVYDCTPFLKDHPGGADSILINAGTDCTEEFEAIHSDKAIKMLEDYKIGDLLIL 600

Query: 601 NENKDATSSPKLISGHVALVPREKIPVKLISKISISHNTRIFRFGLSSEDQTLGLPVGKH 660
           N+NKDATSSPK ++ HVAL+PREKIPVKLISK +ISHNTRIFRFGLSSEDQ LGLPVGKH
Sbjct: 601 NDNKDATSSPKAVTQHVALIPREKIPVKLISKTTISHNTRIFRFGLSSEDQRLGLPVGKH 660

Query: 661 IFLCANIGGKLCMRAYTPSSAVDQMGYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLPLGS 720
           IFLCAN+ GKLCMRAYTPSSAVDQ GYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLPLGS
Sbjct: 661 IFLCANVDGKLCMRAYTPSSAVDQTGYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLPLGS 720

Query: 721 IVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPEDETEM 780
           +V+VKGPLGHIEYMGRGNF V+GKP+ AKKLAMVAGGTGITPIYQVVQAILKDPEDETEM
Sbjct: 721 MVEVKGPLGHIEYMGRGNFRVNGKPRLAKKLAMVAGGTGITPIYQVVQAILKDPEDETEM 780

Query: 781 FVVYANRTEEDILLREELDTWAKENERLKIWYVVKECVKEGWPYSVGYIKESVLREHIPM 840
           FVVYANR+EEDILLREEL+TWAKENERLKIWYVVK+ V+EGWPYSVGYI+E +LREHIPM
Sbjct: 781 FVVYANRSEEDILLREELETWAKENERLKIWYVVKDGVQEGWPYSVGYIREDILREHIPM 840

Query: 841 GSPETLALVCGPPPMIELTVKPILKDLEYDLESSLLVF 878
           GSP+TLALVCGPPPMIELTVKPILK+LEYDLE SLLVF
Sbjct: 841 GSPQTLALVCGPPPMIELTVKPILKNLEYDLEGSLLVF 873

BLAST of HG10009882 vs. NCBI nr
Match: KAE8652087.1 (hypothetical protein Csa_018542 [Cucumis sativus])

HSP 1 Score: 1649.4 bits (4270), Expect = 0.0e+00
Identity = 787/879 (89.53%), Postives = 833/879 (94.77%), Query Frame = 0

Query: 1   MAAAVNHKTLDLEPVSNNANFPVQRCNSFRSSSFKFKRQAEKPLIIDDDDSSFDRDDENS 60
           MA AVNHKTLDLEPVS+  NFPVQRCNSFRS+SFKFKRQAEKPL+IDDDDSSFDRD+EN 
Sbjct: 1   MATAVNHKTLDLEPVSSTGNFPVQRCNSFRSNSFKFKRQAEKPLLIDDDDSSFDRDEENI 60

Query: 61  NYFRELIQKGKEEIEPSILDPRDEGTGDSGIMRNPCMVRLTGKHPFNSEPPLDRLMRHGF 120
           +YFR+LI+KGKEEIEPSILDPRD+GT DSGIMRNPCMVRLTGKHPFNSEPPLD LMRHGF
Sbjct: 61  DYFRDLIRKGKEEIEPSILDPRDQGTADSGIMRNPCMVRLTGKHPFNSEPPLDHLMRHGF 120

Query: 121 ITPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFTLDQLVNDFRSLEFPVTIMCSGN 180
           ITPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFTLDQLVN+FRSLEFPVTIMCSGN
Sbjct: 121 ITPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFTLDQLVNNFRSLEFPVTIMCSGN 180

Query: 181 RRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGGLYIWMEGADDLPG 240
           RRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRG LY+WMEGAD+LPG
Sbjct: 181 RRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALYVWMEGADNLPG 240

Query: 241 GGGCSYGTSIRREMAMDPARDIIIAYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKWLKRI 300
           GGGCSYGTSIRREMAMDP+RDIIIAYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKWLKRI
Sbjct: 241 GGGCSYGTSIRREMAMDPSRDIIIAYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKWLKRI 300

Query: 301 IVTNRESDNFYHFMDNRVLPSHVADSELAHAEGWWYKPEYVIYEMNINSVITTPSHDETL 360
           IVTNRESDN+YHFMDNRVLPSHV  SELAHAEGWWYKPEYVIYEMNINSVITTPSH+ETL
Sbjct: 301 IVTNRESDNYYHFMDNRVLPSHVDSSELAHAEGWWYKPEYVIYEMNINSVITTPSHNETL 360

Query: 361 PINSQTTQSTYTLRG-YAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWCWI 420
            INS+TT STYTLR  +    GGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWCWI
Sbjct: 361 LINSETTLSTYTLRDTHIPVSGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWCWI 420

Query: 421 FWSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQEGEI 480
           FWSV+VEI+DLFGSK+IAVRAWDET NTQPENLNWNLMGMMNNSWYRIKINICKPQ+GEI
Sbjct: 421 FWSVDVEILDLFGSKQIAVRAWDETTNTQPENLNWNLMGMMNNSWYRIKINICKPQKGEI 480

Query: 481 GIVFRHPTLAGNQSGGWKEKGKPIPTNSS-SQPSNNKTTTAATTTTFPMSEVKTHNSPES 540
           GIVFRHPT+AGN +GGW+EK KP PT+ S SQ  +NK     +TTTF MSEVKTHNS ES
Sbjct: 481 GIVFRHPTVAGNNAGGWQEKDKPNPTDESPSQQKDNK-----STTTFSMSEVKTHNSSES 540

Query: 541 AWIVVHNQVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSEKAIKMLEDYKIGDLLI 600
           AWIVV N VYDCTPFLK HPGGADSILINAGTDCTEEF+AIHS+KAIKMLEDYKIGDLLI
Sbjct: 541 AWIVVQNHVYDCTPFLKDHPGGADSILINAGTDCTEEFEAIHSDKAIKMLEDYKIGDLLI 600

Query: 601 LNENKDATSSPKLISGHVALVPREKIPVKLISKISISHNTRIFRFGLSSEDQTLGLPVGK 660
           LN+NKDATSSPK ++ HVAL+PREKIPVKLISK +ISHNTRIFRFGLSSEDQ LGLPVGK
Sbjct: 601 LNDNKDATSSPKAVTQHVALIPREKIPVKLISKTTISHNTRIFRFGLSSEDQRLGLPVGK 660

Query: 661 HIFLCANIGGKLCMRAYTPSSAVDQMGYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLPLG 720
           HIFLCAN+ GKLCMRAYTPSSAVDQ GYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLPLG
Sbjct: 661 HIFLCANVDGKLCMRAYTPSSAVDQTGYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLPLG 720

Query: 721 SIVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPEDETE 780
           S+V+VKGPLGHIEYMGRGNF V+GKP+ AKKLAMVAGGTGITPIYQVVQAILKDPEDETE
Sbjct: 721 SMVEVKGPLGHIEYMGRGNFRVNGKPRLAKKLAMVAGGTGITPIYQVVQAILKDPEDETE 780

Query: 781 MFVVYANRTEEDILLREELDTWAKENERLKIWYVVKECVKEGWPYSVGYIKESVLREHIP 840
           MFVVYANR+EEDILLREEL+TWAKENERLKIWYVVK+ V+EGWPYSVGYI+E +LREHIP
Sbjct: 781 MFVVYANRSEEDILLREELETWAKENERLKIWYVVKDGVQEGWPYSVGYIREDILREHIP 840

Query: 841 MGSPETLALVCGPPPMIELTVKPILKDLEYDLESSLLVF 878
           MGSP+TLALVCGPPPMIELTVKPILK+LEYDLE SLLVF
Sbjct: 841 MGSPQTLALVCGPPPMIELTVKPILKNLEYDLEGSLLVF 874

BLAST of HG10009882 vs. NCBI nr
Match: XP_023551317.1 (nitrate reductase [NADH]-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1604.3 bits (4153), Expect = 0.0e+00
Identity = 768/882 (87.07%), Postives = 820/882 (92.97%), Query Frame = 0

Query: 1   MAAAVNHKTLDLEPVSNNANFPVQRCNSFRSSSFKFKRQAEKPLII----DDDDSSFDRD 60
           MAA V+HKTLD+EP   N NF  QRCNSFRS SFK KR  E  L I    DDDDSSFDR 
Sbjct: 1   MAATVDHKTLDMEP-PTNGNFLAQRCNSFRSPSFKIKRHPEITLTINDDDDDDDSSFDRH 60

Query: 61  DENSNYFRELIQKGKEEIEPSILDPRDEGTGDSGIMRNPCMVRLTGKHPFNSEPPLDRLM 120
           D+N++YFR+LI+KGKEEIEPS+LDPRDEGT D+GI+RNP M+RLTGKHPFNSEPPLDRLM
Sbjct: 61  DDNNDYFRDLIRKGKEEIEPSVLDPRDEGTADNGIIRNPSMIRLTGKHPFNSEPPLDRLM 120

Query: 121 RHGFITPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFTLDQLVNDFRSLEFPVTIM 180
           RHGFITPAPLHYVRNHGPVPK D S+WTI++ GL+ENPT+FTL QLVNDFRS+EFPVTI 
Sbjct: 121 RHGFITPAPLHYVRNHGPVPKADSSNWTIEIIGLLENPTTFTLQQLVNDFRSIEFPVTIT 180

Query: 181 CSGNRRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGGLYIWMEGAD 240
           CSGNRRKEQNM KQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSR RG LY+WMEGAD
Sbjct: 181 CSGNRRKEQNMFKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRHRGALYVWMEGAD 240

Query: 241 DLPGGGGCSYGTSIRREMAMDPARDIIIAYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKW 300
           DLPGGGGCSYGTSIRREMAMDP+RDIIIAY QNGDP+PPDHGFPVRMIVPGFTGGRMVKW
Sbjct: 241 DLPGGGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRMIVPGFTGGRMVKW 300

Query: 301 LKRIIVTNRESDNFYHFMDNRVLPSHVADSELAHAEGWWYKPEYVIYEMNINSVITTPSH 360
           LKRIIVTNRESDN YHFMDNRVLPSHV DSELA+AEGWWYKPEYVIYEMNINSVITTPSH
Sbjct: 301 LKRIIVTNRESDNHYHFMDNRVLPSHV-DSELANAEGWWYKPEYVIYEMNINSVITTPSH 360

Query: 361 DETLPINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYW 420
           DE L INSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYW
Sbjct: 361 DEMLSINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYW 420

Query: 421 CWIFWSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQE 480
           CWIFWSVEVEIV+LFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSW+RIKIN+CKPQ 
Sbjct: 421 CWIFWSVEVEIVELFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWFRIKINVCKPQN 480

Query: 481 GEIGIVFRHPTLAGNQSGGWKEKGKPIPTN-SSSQPSNNKTTTAATTTTFPMSEVKTHNS 540
           GE+GIVFRHPTL GNQSGGWKEK K  PT+  SS+P+N+K  T A   TFPMSEVKTHN+
Sbjct: 481 GELGIVFRHPTLPGNQSGGWKEKDKINPTDPPSSKPTNDKNKTTA-AKTFPMSEVKTHNT 540

Query: 541 PESAWIVVHNQVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSEKAIKMLEDYKIGD 600
           PES+WIVVH  VYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHS+KAI MLEDY IGD
Sbjct: 541 PESSWIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAINMLEDYMIGD 600

Query: 601 LLILNENKDATSSPKLISGHVALVPREKIPVKLISKISISHNTRIFRFGLSSEDQTLGLP 660
           LL  N++++ TSS + +  HVALVP +KIPVKLISKISISHNTRIFRFGLSS+DQ LGLP
Sbjct: 601 LLTENDSEEKTSSSEAVEQHVALVPGKKIPVKLISKISISHNTRIFRFGLSSKDQVLGLP 660

Query: 661 VGKHIFLCANIGGKLCMRAYTPSSAVDQMGYFDLVVKIYFKNVHPDFPNGGLMSQYLDSL 720
           VGKHIFLCANI GKLCMRAYTPSS+VD+MG+FDLVVK+YFKN+HPDFPNGGLMSQYLDSL
Sbjct: 661 VGKHIFLCANIEGKLCMRAYTPSSSVDEMGFFDLVVKVYFKNMHPDFPNGGLMSQYLDSL 720

Query: 721 PLGSIVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPED 780
           PLGSIVDVKGPLGHIEY+GRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPED
Sbjct: 721 PLGSIVDVKGPLGHIEYLGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPED 780

Query: 781 ETEMFVVYANRTEEDILLREELDTWAKENERLKIWYVVKECVKEGWPYSVGYIKESVLRE 840
           ETEM+VVYANRTEEDILLREELDTWAKENERLKIWYVVK+ VKEGWPYSVGYIKESVLRE
Sbjct: 781 ETEMYVVYANRTEEDILLREELDTWAKENERLKIWYVVKDGVKEGWPYSVGYIKESVLRE 840

Query: 841 HIPMGSPETLALVCGPPPMIELTVKPILKDLEYDLESSLLVF 878
           HIPMGSPE+LALVCGPPPMIELTVKPILK++EYDLE+SLLVF
Sbjct: 841 HIPMGSPESLALVCGPPPMIELTVKPILKEMEYDLETSLLVF 879

BLAST of HG10009882 vs. NCBI nr
Match: KAG6578997.1 (hypothetical protein SDJN03_23445, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1591.6 bits (4120), Expect = 0.0e+00
Identity = 766/884 (86.65%), Postives = 818/884 (92.53%), Query Frame = 0

Query: 1   MAAAVNHKTLDLEPVSNNANFPVQRCNSFRSSSFKFKRQAEKPLII------DDDDSSFD 60
           MAA V+HKTLD+EP   N +F  QRCNSFRS SFK KRQ E  L I      DDDDSSFD
Sbjct: 1   MAAVVDHKTLDMEP-PTNGSFLAQRCNSFRSPSFKIKRQPEITLTIDDDDDDDDDDSSFD 60

Query: 61  RDDENSNYFRELIQKGKEEIEPSILDPRDEGTGDSGIMRNPCMVRLTGKHPFNSEPPLDR 120
           R D+N++YFR+LI+KGKEEIEPSILDPRDEGT D+GI+RNP M+RLTGKHPFNSEPPLDR
Sbjct: 61  RHDDNNDYFRDLIRKGKEEIEPSILDPRDEGTADNGILRNPSMIRLTGKHPFNSEPPLDR 120

Query: 121 LMRHGFITPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFTLDQLVNDFRSLEFPVT 180
           LMRHGFITPAPLHYVRNHGPVPK D S+WTI++TGL+ENPT+FTL QLVNDFRS+EFPVT
Sbjct: 121 LMRHGFITPAPLHYVRNHGPVPKADSSNWTIEITGLLENPTTFTLHQLVNDFRSIEFPVT 180

Query: 181 IMCSGNRRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGGLYIWMEG 240
           I CSGNRRKEQNM KQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSR RG LY+WMEG
Sbjct: 181 ITCSGNRRKEQNMFKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRHRGALYVWMEG 240

Query: 241 ADDLPGGGGCSYGTSIRREMAMDPARDIIIAYKQNGDPLPPDHGFPVRMIVPGFTGGRMV 300
           ADDLPGGGGCSYGTSIRREMAMDP+RDIIIAY QNGDP+PPDHGFPVR+IVPGFTGGRMV
Sbjct: 241 ADDLPGGGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRLIVPGFTGGRMV 300

Query: 301 KWLKRIIVTNRESDNFYHFMDNRVLPSHVADSELAHAEGWWYKPEYVIYEMNINSVITTP 360
           KWLKRIIVTNRESDN YHFMDNRVLPSHV DSELA+AEGWWYKPEYVIYEMNINSVITTP
Sbjct: 301 KWLKRIIVTNRESDNHYHFMDNRVLPSHV-DSELANAEGWWYKPEYVIYEMNINSVITTP 360

Query: 361 SHDETLPINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGK 420
           SHDE L I SQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGK
Sbjct: 361 SHDEMLSIKSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGK 420

Query: 421 YWCWIFWSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKP 480
           YWCWIFWSVEVEIV+LFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSW+RIKIN+CKP
Sbjct: 421 YWCWIFWSVEVEIVELFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWFRIKINVCKP 480

Query: 481 QEGEIGIVFRHPTLAGNQSGGWKEKGKPIPTN-SSSQPSNNKTTTAATTTTFPMSEVKTH 540
           Q GE+GIVFRHPTL GNQSGGWKEK K  PT+ SSS+P+N+K  T A + TF MSEVKTH
Sbjct: 481 QNGELGIVFRHPTLPGNQSGGWKEKDKINPTDPSSSKPTNDKNKTTA-SKTFAMSEVKTH 540

Query: 541 NSPESAWIVVHNQVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSEKAIKMLEDYKI 600
           N+PES+WIVVH  VYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHS+KAI ML+DY I
Sbjct: 541 NTPESSWIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAINMLQDYII 600

Query: 601 GDLLILNENKDATSSPKLISGHVALVPREKIPVKLISKISISHNTRIFRFGLSSEDQTLG 660
           GDLL  N+N++ TSS + +  HVALVP +KIPVKLISKISIS NTRIFRFGLSS+DQ LG
Sbjct: 601 GDLLTENDNEENTSSSEPVEQHVALVPGKKIPVKLISKISISQNTRIFRFGLSSKDQVLG 660

Query: 661 LPVGKHIFLCANIGGKLCMRAYTPSSAVDQMGYFDLVVKIYFKNVHPDFPNGGLMSQYLD 720
           LPVGKHIFLCA I GKLCMRAYTPSS+VD+MG+FDLVVK+YFKNVHPDFPNGGLMSQYLD
Sbjct: 661 LPVGKHIFLCAIIEGKLCMRAYTPSSSVDEMGFFDLVVKVYFKNVHPDFPNGGLMSQYLD 720

Query: 721 SLPLGSIVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDP 780
           SLPLGS VDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDP
Sbjct: 721 SLPLGSTVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDP 780

Query: 781 EDETEMFVVYANRTEEDILLREELDTWAKENERLKIWYVVKECVKEGWPYSVGYIKESVL 840
           ED+TEM+VVYANRT EDILLREELDTWAKENERLKIWYVVKE VKEGWPYSVGYIKESVL
Sbjct: 781 EDKTEMYVVYANRTVEDILLREELDTWAKENERLKIWYVVKEGVKEGWPYSVGYIKESVL 840

Query: 841 REHIPMGSPETLALVCGPPPMIELTVKPILKDLEYDLESSLLVF 878
           REHIPMGSPE+LALVCGPPPMIELTVKPILK++EYDLE+SLLVF
Sbjct: 841 REHIPMGSPESLALVCGPPPMIELTVKPILKEMEYDLETSLLVF 881

BLAST of HG10009882 vs. ExPASy Swiss-Prot
Match: P17569 (Nitrate reductase [NADH] OS=Cucurbita maxima OX=3661 PE=2 SV=1)

HSP 1 Score: 1242.6 bits (3214), Expect = 0.0e+00
Identity = 586/897 (65.33%), Postives = 714/897 (79.60%), Query Frame = 0

Query: 16  SNNANFPVQRCNSFRS-----------SSFKFKRQAEKPLIIDDDDSSFDRDDENSNYFR 75
           ++ A+ PV+ CN   S           SS K +  A + +   +D  S   +DEN N FR
Sbjct: 29  NHRADSPVRGCNFPNSNVDYNNNRPLKSSVKIQEAAAEEM---EDSCS---EDENENEFR 88

Query: 76  ELIQKGKEEIEPSILDPRDEGTGDSGIMRNPCMVRLTGKHPFNSEPPLDRLMRHGFITPA 135
           +LI KG  E+EPSILD RDEGT D+ I RN  MVRLTGKHPFNSEPPL+RLM HGFITP 
Sbjct: 89  DLIVKGNRELEPSILDHRDEGTADNWIERNASMVRLTGKHPFNSEPPLNRLMHHGFITPV 148

Query: 136 PLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFTLDQLVNDFRSLEFPVTIMCSGNRRKE 195
           PLHYVRNHG VPK  W+ WT++V GLV+ P  FT+DQL+N+FR  EFP T++C+GNRRKE
Sbjct: 149 PLHYVRNHGVVPKAKWADWTVEVCGLVKRPAKFTMDQLLNEFRFREFPATLVCAGNRRKE 208

Query: 196 QNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGGLYIWMEGADDLPGGGGC 255
           QNMVKQ+IGF+WGAA  STS+WR VPL ++LKRCGI SR++G L +  EGA+DLPGGGG 
Sbjct: 209 QNMVKQSIGFNWGAAGVSTSVWRRVPLCDLLKRCGILSRKKGALNVCFEGAEDLPGGGGS 268

Query: 256 SYGTSIRREMAMDPARDIIIAYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKWLKRIIVTN 315
            YGTSI++E+AMDPARDII+AY QNG+ L PDHGFPVRMI+PGF GGRMVKWLKRIIVT 
Sbjct: 269 KYGTSIKKELAMDPARDIILAYMQNGEQLAPDHGFPVRMIIPGFIGGRMVKWLKRIIVTT 328

Query: 316 RESDNFYHFMDNRVLPSHVADSELAHAEGWWYKPEYVIYEMNINSVITTPSHDETLPINS 375
           +ES+N+YHF DNRVLPSHV D+++A+AE WWYKPE++I E+NINSVITTP H+E LPIN+
Sbjct: 329 KESENYYHFKDNRVLPSHV-DADVANAEAWWYKPEHIINELNINSVITTPCHEEILPINA 388

Query: 376 QTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWCWIFWSVE 435
            TTQ  YTLRGY+YSGGGKKVTRV+VT+D GETW++C L++ EK NKYGKYWCW FWS+E
Sbjct: 389 WTTQRPYTLRGYSYSGGGKKVTRVEVTMDSGETWQVCTLDHPEKANKYGKYWCWCFWSLE 448

Query: 436 VEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQEGEIGIVFR 495
           VE++DL  +KEIAVRAWDET+NTQPE L WNLMGMMNN W+R+K N+CKP +GEIGIVF 
Sbjct: 449 VEVLDLLSAKEIAVRAWDETHNTQPEKLIWNLMGMMNNCWFRVKTNMCKPHKGEIGIVFE 508

Query: 496 HPTLAGNQSGGWKEKGKPIPTNSSSQPSNNKTTTA----ATTTTFPMSEVKTHNSPESAW 555
           HPT  GNQSGGW ++ + +  ++ S  +  K+ +       + T+ +SEVK HNSP+SAW
Sbjct: 509 HPTQPGNQSGGWMDRERHLEISTESNQTLKKSVSTPFMNTASNTYTLSEVKKHNSPQSAW 568

Query: 556 IVVHNQVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSEKAIKMLEDYKIGDLLILN 615
           I+VH  VYDCT FLK HPGG+DSILINAGTDCTEEFDAIHS+KA KMLEDY+IG+L+   
Sbjct: 569 IIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDKAKKMLEDYRIGELITTG 628

Query: 616 ENKDATSSPKLISGH-------------------VALVPREKIPVKLISKISISHNTRIF 675
              D++S+    S H                   VAL P EKIP KLISK SISH+ R+F
Sbjct: 629 YASDSSSNSPNNSTHGASNFSHLAPIREAPVSRRVALAPNEKIPCKLISKTSISHDVRVF 688

Query: 676 RFGL-SSEDQTLGLPVGKHIFLCANIGGKLCMRAYTPSSAVDQMGYFDLVVKIYFKNVHP 735
           RF L   +DQ LGLPVGKHIF+CA + GKLCMRAYTP+S++D+MG+F+LVVK+YFK VHP
Sbjct: 689 RFALPGGQDQALGLPVGKHIFICATVDGKLCMRAYTPTSSIDEMGFFELVVKVYFKGVHP 748

Query: 736 DFPNGGLMSQYLDSLPLGSIVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGIT 795
            FPNGG+MSQYLDS+ +GS +DVKGPLGHIEY GRGNF VHGKP+FA++LAM+AGGTGIT
Sbjct: 749 KFPNGGIMSQYLDSMEVGSTLDVKGPLGHIEYTGRGNFMVHGKPRFARRLAMLAGGTGIT 808

Query: 796 PIYQVVQAILKDPEDETEMFVVYANRTEEDILLREELDTWAKENERLKIWYVVKECVKEG 855
           PIYQVVQAILKDPEDETEM+VVYANRTE+DILLR+ELDTWAK+N+RLK+WYVV+E ++EG
Sbjct: 809 PIYQVVQAILKDPEDETEMYVVYANRTEDDILLRDELDTWAKKNQRLKVWYVVQESIREG 868

Query: 856 WPYSVGYIKESVLREHIPMGSPETLALVCGPPPMIELTVKPILKDLEYDLESSLLVF 878
           W YSVG+I E++LREHIP  + +TLAL CGPP MI+  V+P L+ + YD ++SLLVF
Sbjct: 869 WEYSVGFITENILREHIPAAAEDTLALACGPPAMIQFAVQPNLEKMNYDTKNSLLVF 918

BLAST of HG10009882 vs. ExPASy Swiss-Prot
Match: P27783 (Nitrate reductase [NAD(P)H] OS=Betula pendula OX=3505 GN=NIA1 PE=2 SV=1)

HSP 1 Score: 1237.6 bits (3201), Expect = 0.0e+00
Identity = 587/873 (67.24%), Postives = 700/873 (80.18%), Query Frame = 0

Query: 27  NSFRSSSFKFKRQ---AEKPLIIDDDDSSFDRDDENSNYFRELIQKGKEEIEPSILDPRD 86
           NS RS S    RQ   + K  +   +DSS   +DEN N ++ELIQKG  E+EPSILDPRD
Sbjct: 29  NSHRSDSPDLGRQIPSSPKKQVATGEDSS--SEDENENDYKELIQKGNGELEPSILDPRD 88

Query: 87  EGTGDSGIMRNPCMVRLTGKHPFNSEPPLDRLMRHGFITPAPLHYVRNHGPVPKTDWSSW 146
           E T D+ + RN  MVRLTGKHPFNSE PL RLM HGFITPAPLHYVRNHGPVPK  W  W
Sbjct: 89  EATADNWVERNATMVRLTGKHPFNSEAPLTRLMHHGFITPAPLHYVRNHGPVPKARWEDW 148

Query: 147 TIDVTGLVENPTSFTLDQLVNDFRSLEFPVTIMCSGNRRKEQNMVKQTIGFHWGAAVTST 206
           +++V GLV+ P  FT+D+LV +FRS EFPVT++C+GNRRKEQNMVK+TIGF+WGAA  ST
Sbjct: 149 SVEVCGLVKRPARFTMDRLVTEFRSREFPVTLVCAGNRRKEQNMVKKTIGFNWGAAGVST 208

Query: 207 SLWRGVPLREVLKRCGIFSRQRGGLYIWMEGADDLPGGGGCSYGTSIRREMAMDPARDII 266
           S+WRGVPLR+VLKRCGIFSR RG   +  EGA+DLPGGGG  YGTS++ EMAMDPARDII
Sbjct: 209 SVWRGVPLRDVLKRCGIFSRGRGAFNVCFEGAEDLPGGGGSKYGTSVKYEMAMDPARDII 268

Query: 267 IAYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKWLKRIIVTNRESDNFYHFMDNRVLPSHV 326
           + Y QNG+ L PDHGFPVRMI+PGF GGRMVKWLKRIIVT +ESDN+YH+ DNRVLPSHV
Sbjct: 269 LGYMQNGERLSPDHGFPVRMIIPGFIGGRMVKWLKRIIVTTKESDNYYHYNDNRVLPSHV 328

Query: 327 ADSELAHAEGWWYKPEYVIYEMNINSVITTPSHDETLPINSQTTQSTYTLRGYAYSGGGK 386
            D+++A AE WWYKPE++I E+NINSVITTP H+E LPINS TTQ  YTLRGYAYSGGG+
Sbjct: 329 -DADVAKAEAWWYKPEHIINELNINSVITTPCHEEILPINSWTTQRPYTLRGYAYSGGGR 388

Query: 387 KVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWCWIFWSVEVEIVDLFGSKEIAVRAWDE 446
           KVTRV++T++GGE W++C L++ EKPNKYGKYWCW FWS+EVE++DL G+KEIAVRAWDE
Sbjct: 389 KVTRVEITMNGGEKWRVCALDHPEKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDE 448

Query: 447 TNNTQPENLNWNLMGMMNNSWYRIKINICKPQEGEIGIVFRHPTLAGNQSGGWKEKGKPI 506
            +NTQPE L WN+MGMMNN W+R+K N+CK   GEIGI F HPT+ GN+SGGW  + K +
Sbjct: 449 AHNTQPEKLIWNVMGMMNNCWFRVKTNVCKAHMGEIGIAFEHPTVPGNESGGWMAREKNL 508

Query: 507 PTNSSSQPSNNKTTTAATTTT----FPMSEVKTHNSPESAWIVVHNQVYDCTPFLKAHPG 566
            T+S +  S  K+ ++    T    F MSEVK HNS ESAWI+VH  +YDCT FLK HPG
Sbjct: 509 ETSSDANQSLKKSVSSPFMNTSSKMFSMSEVKKHNSAESAWIIVHGHIYDCTHFLKDHPG 568

Query: 567 GADSILINAGTDCTEEFDAIHSEKAIKMLEDYKIGDLLILNENKDATSSP---------- 626
           GADSILINAGTDCTEEFDAIHS+KA KMLEDY+IG+L+      D+ +S           
Sbjct: 569 GADSILINAGTDCTEEFDAIHSDKAKKMLEDYRIGELITTGYVSDSPNSTVHGASNTSHL 628

Query: 627 ---KLIS--GHVALVPREKIPVKLISKISISHNTRIFRFGLSSEDQTLGLPVGKHIFLCA 686
              K I+   +VAL+P  KIP KL+ K S+SH+ R+FR  L S+DQ LGLPVGKH+FLCA
Sbjct: 629 APIKEIAPLRNVALIPGAKIPTKLVYKKSLSHDVRLFRLALPSDDQVLGLPVGKHVFLCA 688

Query: 687 NIGGKLCMRAYTPSSAVDQMGYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLPLGSIVDVK 746
            I  KLCMRAYTP+S +D++GY DLVVKIYFKN +P FPNGGLMSQ+LDSLP+GS++ VK
Sbjct: 689 TIDDKLCMRAYTPTSTIDEVGYLDLVVKIYFKNSNPRFPNGGLMSQHLDSLPIGSVLHVK 748

Query: 747 GPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPEDETEMFVVYA 806
           GPLGH+EY GRGNF VHG+PKFAK+LAMVAGGTGITPIYQV+QAILKDPEDETEMFVVYA
Sbjct: 749 GPLGHVEYTGRGNFLVHGEPKFAKRLAMVAGGTGITPIYQVIQAILKDPEDETEMFVVYA 808

Query: 807 NRTEEDILLREELDTWAKENERLKIWYVVKECVKEGWPYSVGYIKESVLREHIPMGSPET 866
           NRTE+DILLREELD WAK++E+LK+WYVVKE  +EGW YSVGYI+ES+LREHIP GS + 
Sbjct: 809 NRTEDDILLREELDDWAKKHEKLKVWYVVKESKREGWEYSVGYIRESILREHIPEGSDDV 868

Query: 867 LALVCGPPPMIELTVKPILKDLEYDLESSLLVF 878
           LAL CG P MIE  V+  L+ + YD ++SL++F
Sbjct: 869 LALACGAPSMIEEAVRLNLEKMNYDTKNSLIIF 898

BLAST of HG10009882 vs. ExPASy Swiss-Prot
Match: P39865 (Nitrate reductase [NADH] 1 OS=Phaseolus vulgaris OX=3885 GN=NIA1 PE=3 SV=1)

HSP 1 Score: 1226.1 bits (3171), Expect = 0.0e+00
Identity = 568/892 (63.68%), Postives = 714/892 (80.04%), Query Frame = 0

Query: 1   MAAAVNHKTLDLEPVSNNANFPVQRCNSFRSSSFKFKRQAEKPLIIDDDDSSFDRDDENS 60
           MAA+V H+     P +++ +  V+   S+       K    +PL     DS+ D D+E +
Sbjct: 1   MAASVEHR-----PFTSHQHGVVRSFKSYPDVPRPKKLPLPQPL----SDSTNDNDEE-A 60

Query: 61  NYFRELIQKGKEEIEPSILDPRDEGTGDSGIMRNPCMVRLTGKHPFNSEPPLDRLMRHGF 120
             ++EL+ K    +EPSI DPRD+GT D  I RN  M+RLTGKHPFN+EPPL RLM+HGF
Sbjct: 61  AVWKELVLKSSAGVEPSIFDPRDDGTSDQWIKRNASMIRLTGKHPFNAEPPLPRLMQHGF 120

Query: 121 ITPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFTLDQLVNDFRSLEFPVTIMCSGN 180
           ITP+P+HYVR+HGPVP   W  WT++VTGLV  PT FT++QLVNDF S EFP T++C+GN
Sbjct: 121 ITPSPIHYVRSHGPVPHARWEDWTVEVTGLVTRPTCFTMEQLVNDFPSHEFPATLVCAGN 180

Query: 181 RRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGGLYIWMEGADDLPG 240
           RRKEQNMVKQ+IGF+WGA   STS+WRGV LR +LKRCGI+SR +G L++  EGA+DLPG
Sbjct: 181 RRKEQNMVKQSIGFNWGAGGVSTSVWRGVSLRSLLKRCGIYSRAKGALHVCFEGAEDLPG 240

Query: 241 GGGCSYGTSIRREMAMDPARDIIIAYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKWLKRI 300
           GGG +YGTS+ RE+A+DP+RDII+AY QNG+ L PDHGFPVRMI+PGF GGRMVKWLKRI
Sbjct: 241 GGGSNYGTSLMREVALDPSRDIILAYMQNGELLSPDHGFPVRMIIPGFIGGRMVKWLKRI 300

Query: 301 IVTNRESDNFYHFMDNRVLPSHVADSELAHAEGWWYKPEYVIYEMNINSVITTPSHDETL 360
           +V+N++S + YH+ DN++ PSHV D+ELA+ E WWYKPEY+I E+NINSVITTPSH E L
Sbjct: 301 VVSNQQSQSHYHYKDNKLFPSHV-DAELANEEDWWYKPEYIINEVNINSVITTPSHQEIL 360

Query: 361 PINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWCWIF 420
           PINS TTQ  Y++RGYAYSGGG+KVTRV+VTLDGGETW++C++E  EKPNKYGKYWCW F
Sbjct: 361 PINSWTTQMPYSMRGYAYSGGGRKVTRVEVTLDGGETWQVCSVERLEKPNKYGKYWCWCF 420

Query: 421 WSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQEGEIG 480
           WS+EVE++D+ G+KEIAVRAWDE  NTQPE L WN MGM+NN W+R+K N+CKP++GEIG
Sbjct: 421 WSLEVEVLDILGAKEIAVRAWDEAQNTQPEKLIWNTMGMINNCWFRVKTNVCKPKKGEIG 480

Query: 481 IVFRHPTLAGNQSGGWKEKGKPIPTNSSSQPSNNKTTTA----ATTTTFPMSEVKTHNSP 540
           IVF HPT  GNQSGGW  + K +  +S S P   K+ ++      T ++ +SEV+ HN+ 
Sbjct: 481 IVFEHPTQPGNQSGGWMAREKQLEKSSESNPILKKSVSSPFMNTATKSYSLSEVRRHNNR 540

Query: 541 ESAWIVVHNQVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSEKAIKMLEDYKIGDL 600
           +SAWI+V+  VYDCT FLK HPGG DSIL+NAGTDCTEEF+AIHS+KA KMLEDY+IG+L
Sbjct: 541 DSAWIIVNGHVYDCTRFLKDHPGGEDSILLNAGTDCTEEFEAIHSDKAKKMLEDYRIGEL 600

Query: 601 LILNENKDATSSPKLISGH-----------VALVPREKIPVKLISKISISHNTRIFRFGL 660
           +  +   D++SS   + G+           VAL PREKIP KL+SK SISH+ R+ RF L
Sbjct: 601 MTTDYTSDSSSSNNSVHGNSETTHLAPIREVALNPREKIPCKLLSKTSISHDVRLLRFAL 660

Query: 661 SSEDQTLGLPVGKHIFLCANIGGKLCMRAYTPSSAVDQMGYFDLVVKIYFKNVHPDFPNG 720
            +EDQ +GLPVG H+FLCA +  KLCMRAYTP+S+VD++G+FDLVVK+YFK VHP+FPNG
Sbjct: 661 PAEDQVMGLPVGNHVFLCATVDEKLCMRAYTPTSSVDEVGFFDLVVKVYFKGVHPNFPNG 720

Query: 721 GLMSQYLDSLPLGSIVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQV 780
           G+MSQ+LDSLP+GS+VDVKGPLGHIEY GRGNF VHGKP+FAK+L M+AGGTGITPIYQV
Sbjct: 721 GIMSQHLDSLPIGSVVDVKGPLGHIEYTGRGNFLVHGKPRFAKRLTMLAGGTGITPIYQV 780

Query: 781 VQAILKDPEDETEMFVVYANRTEEDILLREELDTWAKENERLKIWYVVKECVKEGWPYSV 840
           VQAILKDPED TEM+VVYANRTE+DILL+EELD WAK+++RLK+WYV++  ++EGW YSV
Sbjct: 781 VQAILKDPEDRTEMYVVYANRTEDDILLKEELDEWAKKHDRLKVWYVLQANIREGWEYSV 840

Query: 841 GYIKESVLREHIPMGSPETLALVCGPPPMIELTVKPILKDLEYDLESSLLVF 878
           G+I ES+LREH+P+ SP+TLAL CGPPPMI+  V+P L+ L YD+++ LLVF
Sbjct: 841 GFITESILREHVPLASPDTLALTCGPPPMIQFAVQPNLEKLGYDIQNDLLVF 881

BLAST of HG10009882 vs. ExPASy Swiss-Prot
Match: P08509 (Nitrate reductase [NADH] 2 OS=Nicotiana tabacum OX=4097 GN=NIA2 PE=2 SV=2)

HSP 1 Score: 1225.3 bits (3169), Expect = 0.0e+00
Identity = 573/878 (65.26%), Postives = 694/878 (79.04%), Query Frame = 0

Query: 22  PVQRCNSFRSSSFKFKRQAEKPLIIDDDDSSFDRDDENSNYFRELIQKGKEEIEPSILDP 81
           PV+ CN    +S  F+++    + +D   S  D DD+  N + ++I+KG  E+EPS+ D 
Sbjct: 28  PVRGCNFPSPNSTNFQKKPNSTIYLDYSSSEDDDDDDEKNEYLQMIKKGNSELEPSVHDT 87

Query: 82  RDEGTGDSGIMRNPCMVRLTGKHPFNSEPPLDRLMRHGFITPAPLHYVRNHGPVPKTDWS 141
           RDEGT D+ I RN  M+RLTGKHPFNSEPPL+RLM HGFITP PLHYVRNHGPVPK  W 
Sbjct: 88  RDEGTADNWIERNFSMIRLTGKHPFNSEPPLNRLMHHGFITPVPLHYVRNHGPVPKGTWD 147

Query: 142 SWTIDVTGLVENPTSFTLDQLVNDFRSLEFPVTIMCSGNRRKEQNMVKQTIGFHWGAAVT 201
            WT++VTGLV+ P  FT+DQLVN+F   E PVT++C+GNRRKEQNMVKQTIGF+WGAA  
Sbjct: 148 DWTVEVTGLVKRPMKFTMDQLVNEFPCRELPVTLVCAGNRRKEQNMVKQTIGFNWGAAAV 207

Query: 202 STSLWRGVPLREVLKRCGIFSRQRGGLYIWMEGADDLPGGGGCSYGTSIRREMAMDPARD 261
           ST++WRGVPLR +LKRCG+FS+ +G L +  EGAD LPGGGG  YGTSI++E AMDPARD
Sbjct: 208 STTIWRGVPLRALLKRCGVFSKNKGALNVCFEGADVLPGGGGSKYGTSIKKEFAMDPARD 267

Query: 262 IIIAYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKWLKRIIVTNRESDNFYHFMDNRVLPS 321
           II+AY QNG+ L PDHGFPVRMI+PGF GGRMVKW+KRIIVT +ESD++YHF DNRVLP 
Sbjct: 268 IIVAYMQNGEKLAPDHGFPVRMIIPGFIGGRMVKWIKRIIVTTQESDSYYHFKDNRVLPP 327

Query: 322 HVADSELAHAEGWWYKPEYVIYEMNINSVITTPSHDETLPINSQTTQSTYTLRGYAYSGG 381
           HV D+ELA+ E WWYKPEY+I E+NINSVITTP H+E LPIN+ TTQ  YTLRGY+YSGG
Sbjct: 328 HV-DAELANTEAWWYKPEYIINELNINSVITTPCHEEILPINAWTTQRPYTLRGYSYSGG 387

Query: 382 GKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWCWIFWSVEVEIVDLFGSKEIAVRAW 441
           GKKVTRV+VTLDGGETW++  L++ EKP KYGKYWCW FWS+EVE++DL  +KEIAVRAW
Sbjct: 388 GKKVTRVEVTLDGGETWQVSTLDHPEKPTKYGKYWCWCFWSLEVEVLDLLSAKEIAVRAW 447

Query: 442 DETNNTQPENLNWNLMGMMNNSWYRIKINICKPQEGEIGIVFRHPTLAGNQSGGWKEKGK 501
           DET NTQPE L WN+MGMMNN W+R+K+N+CKP +GEIGIVF HPT  GNQSGGW  K +
Sbjct: 448 DETLNTQPEKLIWNVMGMMNNCWFRVKMNVCKPHKGEIGIVFEHPTQPGNQSGGWMAKER 507

Query: 502 PIPTNSSSQPSNNKTTTAATTTT----FPMSEVKTHNSPESAWIVVHNQVYDCTPFLKAH 561
            +  ++ +  +  K+ +     T    + MSEV+ H+S +SAWI+VH  +YD T FLK H
Sbjct: 508 HLEISAEAPQTLKKSISTPFMNTASKMYSMSEVRKHSSADSAWIIVHGHIYDATRFLKDH 567

Query: 562 PGGADSILINAGTDCTEEFDAIHSEKAIKMLEDYKIGDLLILNENKDATSS--------- 621
           PGG DSILINAGTDCTEEFDAIHS+KA K+LED++IG+L+      D+  +         
Sbjct: 568 PGGTDSILINAGTDCTEEFDAIHSDKAKKLLEDFRIGELITTGYTSDSPGNSVHGSSSFS 627

Query: 622 ------PKLISGH--VALVPREKIPVKLISKISISHNTRIFRFGLSSEDQTLGLPVGKHI 681
                  +L+     VAL+PREKIP KLI K SISH+ R FRF L SEDQ LGLPVGKHI
Sbjct: 628 SFLAPIKELVPAQRSVALIPREKIPCKLIDKQSISHDVRKFRFALPSEDQVLGLPVGKHI 687

Query: 682 FLCANIGGKLCMRAYTPSSAVDQMGYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLPLGSI 741
           FLCA I  KLCMRAYTP+S +D++GYF+LVVKIYFK +HP FPNGG MSQYLDS+PLGS 
Sbjct: 688 FLCAVIDDKLCMRAYTPTSTIDEVGYFELVVKIYFKGIHPKFPNGGQMSQYLDSMPLGSF 747

Query: 742 VDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPEDETEMF 801
           +DVKGPLGHIEY G+GNF VHGK KFAKKLAM+AGGTGITP+YQV+QAILKDPED+TEM+
Sbjct: 748 LDVKGPLGHIEYQGKGNFLVHGKQKFAKKLAMIAGGTGITPVYQVMQAILKDPEDDTEMY 807

Query: 802 VVYANRTEEDILLREELDTWA-KENERLKIWYVVKECVKEGWPYSVGYIKESVLREHIPM 861
           VVYANRTE+DILL+EELD+WA K  ER+K+WYVV++ +KEGW YS+G+I E++LREHIP 
Sbjct: 808 VVYANRTEDDILLKEELDSWAEKIPERVKVWYVVQDSIKEGWKYSIGFITEAILREHIPE 867

Query: 862 GSPETLALVCGPPPMIELTVKPILKDLEYDLESSLLVF 878
            S  TLAL CGPPPMI+  V P L+ + YD++ SLLVF
Sbjct: 868 PSHTTLALACGPPPMIQFAVNPNLEKMGYDIKDSLLVF 904

BLAST of HG10009882 vs. ExPASy Swiss-Prot
Match: P39870 (Inducible nitrate reductase [NADH] 2 OS=Glycine max OX=3847 GN=INR2 PE=2 SV=1)

HSP 1 Score: 1224.5 bits (3167), Expect = 0.0e+00
Identity = 571/861 (66.32%), Postives = 690/861 (80.14%), Query Frame = 0

Query: 39  QAEKPLIIDDDDSSFDRDDENSNYFRELIQKGKEEIEPSILDPRDEGTGDSGIMRNPCMV 98
           QA  PL     DSS D +++ +   ++LI+KG  E+E SI DPRD+GT D  I RN  +V
Sbjct: 35  QAPPPL----SDSSSDEEEDTTLNLKDLIRKGTTEVESSIFDPRDDGTSDHWIQRNSSLV 94

Query: 99  RLTGKHPFNSEPPLDRLMRHGFITPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFT 158
           RLTGKHPFNSEPPL RLM HGFITP PLHYVRNHGPVP+  W  WT++VTGLV  PT FT
Sbjct: 95  RLTGKHPFNSEPPLPRLMHHGFITPVPLHYVRNHGPVPRARWEDWTVEVTGLVTRPTCFT 154

Query: 159 LDQLVNDFRSLEFPVTIMCSGNRRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRC 218
           ++QL++DF S EFP T++C+GNRRKEQNMVKQ+IGF+WGAA  STS+WRGVPLR +LK C
Sbjct: 155 MEQLLHDFPSREFPATLVCAGNRRKEQNMVKQSIGFNWGAAAISTSVWRGVPLRTLLKSC 214

Query: 219 GIFSRQRGGLYIWMEGADDLPGGGGCSYGTSIRREMAMDPARDIIIAYKQNGDPLPPDHG 278
           GI++R +G L++  EGA+DLPGGGG  YGTSI RE+A+DP+RDII+AY QNG+PL PDHG
Sbjct: 215 GIYTRTKGALHVCFEGAEDLPGGGGSKYGTSILREVALDPSRDIILAYMQNGEPLSPDHG 274

Query: 279 FPVRMIVPGFTGGRMVKWLKRIIVTNRESDNFYHFMDNRVLPSHVADSELAHAEGWWYKP 338
           FPVRMI+PGF GGRMVKWLKRIIVT  +S N+YH+ DNRVLPSHV D+ELA+A+ WWYKP
Sbjct: 275 FPVRMIIPGFIGGRMVKWLKRIIVTTDQSQNYYHYKDNRVLPSHV-DAELANAQAWWYKP 334

Query: 339 EYVIYEMNINSVITTPSHDETLPINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETW 398
           +Y+I E+NINSVITTP H+E LPINS TTQ  Y +RGYAYSGGG+KVTRV+VTLDGGETW
Sbjct: 335 DYIINELNINSVITTPCHEEILPINSWTTQMPYFIRGYAYSGGGRKVTRVEVTLDGGETW 394

Query: 399 KICNLEYHEKPNKYGKYWCWIFWSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMG 458
           ++C L+  EKPNKYGKYWCW  WSVEVE++DL G++EIAVRAWDE  NTQPE L WN+MG
Sbjct: 395 QVCTLDCPEKPNKYGKYWCWCLWSVEVEVLDLLGAREIAVRAWDEALNTQPEKLIWNVMG 454

Query: 459 MMNNSWYRIKINICKPQEGEIGIVFRHPTLAGNQSGGWKEKGKPIPTNSSSQPSNNKTTT 518
           MMNN W+R+K N+C+P +GEIGIVF HPT  GNQSGGW  K K +  +S S P+  K+ +
Sbjct: 455 MMNNCWFRVKTNVCRPHKGEIGIVFEHPTQPGNQSGGWMAKEKHLEKSSESNPTLKKSVS 514

Query: 519 A----ATTTTFPMSEVKTHNSPESAWIVVHNQVYDCTPFLKAHPGGADSILINAGTDCTE 578
           +     T+ T+ MSEV+ HN+ +SAWI+VH  VYDCT FLK HPGG DSILINAGTDCTE
Sbjct: 515 SPFMNTTSKTYTMSEVRRHNNADSAWIIVHGHVYDCTRFLKDHPGGTDSILINAGTDCTE 574

Query: 579 EFDAIHSEKAIKMLEDYKIGDLLILNENKDATSSPKLISG------------------HV 638
           EF+AIHS+KA +MLEDY+IG+L     N D++SS   + G                   V
Sbjct: 575 EFEAIHSDKAKQMLEDYRIGELTTTCYNSDSSSSNPSVHGSSDTIPLTPIKEVITPMRSV 634

Query: 639 ALVPREKIPVKLISKISISHNTRIFRFGLSSEDQTLGLPVGKHIFLCANIGGKLCMRAYT 698
           AL PREKIP KLISK SISH+ R+FRF L S+D  +GLPVGKHIFLCA +  KLCMRAYT
Sbjct: 635 ALNPREKIPCKLISKTSISHDVRLFRFALPSDDLLMGLPVGKHIFLCATVDEKLCMRAYT 694

Query: 699 PSSAVDQMGYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLPLGSIVDVKGPLGHIEYMGRG 758
           P+S+V ++GYFDLVVK+YFK VHP FP GG+MSQ+LDSLP+GS++DVKGPLGHI Y GRG
Sbjct: 695 PTSSVHEVGYFDLVVKVYFKGVHPKFPTGGIMSQHLDSLPIGSVLDVKGPLGHIVYTGRG 754

Query: 759 NFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEEDILLREE 818
           NF VHGKP+FA +LAM+AGGTGITPIYQVV+AILKDPED TEM VVYANRTE+DILL+EE
Sbjct: 755 NFLVHGKPRFATRLAMLAGGTGITPIYQVVRAILKDPEDCTEMHVVYANRTEDDILLKEE 814

Query: 819 LDTWAKENERLKIWYVVKECVKEGWPYSVGYIKESVLREHIPMGSPETLALVCGPPPMIE 878
           LD WAK+ +RLK+WYV++  ++EGW YSVG+I ES+L EHIP  SP+TLAL CGPPPMI+
Sbjct: 815 LDEWAKKYDRLKVWYVIQASIREGWEYSVGFITESILTEHIPNASPDTLALTCGPPPMIQ 874

BLAST of HG10009882 vs. ExPASy TrEMBL
Match: I0CCB0 (Nitrate reductase OS=Cucumis sativus OX=3659 GN=NR3 PE=2 SV=1)

HSP 1 Score: 1669.1 bits (4321), Expect = 0.0e+00
Identity = 793/878 (90.32%), Postives = 838/878 (95.44%), Query Frame = 0

Query: 1   MAAAVNHKTLDLEPVSNNANFPVQRCNSFRSSSFKFKRQAEKPLIIDDDDSSFDRDDENS 60
           MA AVNHKTLDLEPVS+  NFPVQRCNSFRS+SFKFKRQAEKPL+IDDDDSSFDRD+EN 
Sbjct: 1   MATAVNHKTLDLEPVSSTGNFPVQRCNSFRSNSFKFKRQAEKPLLIDDDDSSFDRDEENI 60

Query: 61  NYFRELIQKGKEEIEPSILDPRDEGTGDSGIMRNPCMVRLTGKHPFNSEPPLDRLMRHGF 120
           +YFR+LI+KGKEEIEPSILDPRD+GT DSGIMRNPCMVRLTGKHPFNSEPPLD LMRHGF
Sbjct: 61  DYFRDLIRKGKEEIEPSILDPRDQGTADSGIMRNPCMVRLTGKHPFNSEPPLDHLMRHGF 120

Query: 121 ITPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFTLDQLVNDFRSLEFPVTIMCSGN 180
           ITPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFTLDQLVN+FRSLEFPVTIMCSGN
Sbjct: 121 ITPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFTLDQLVNNFRSLEFPVTIMCSGN 180

Query: 181 RRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGGLYIWMEGADDLPG 240
           RRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRG LY+WMEGAD+LPG
Sbjct: 181 RRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALYVWMEGADNLPG 240

Query: 241 GGGCSYGTSIRREMAMDPARDIIIAYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKWLKRI 300
           GGGCSYGTSIRREMAMDP+RDIIIAYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKWLKRI
Sbjct: 241 GGGCSYGTSIRREMAMDPSRDIIIAYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKWLKRI 300

Query: 301 IVTNRESDNFYHFMDNRVLPSHVADSELAHAEGWWYKPEYVIYEMNINSVITTPSHDETL 360
           IVTNRESDN+YHFMDNRVLPSHV  SELAHAEGWWYKPEYVIYEMNINSVITTPSH+ETL
Sbjct: 301 IVTNRESDNYYHFMDNRVLPSHVDSSELAHAEGWWYKPEYVIYEMNINSVITTPSHNETL 360

Query: 361 PINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWCWIF 420
            INS+TT STYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWCWIF
Sbjct: 361 LINSETTLSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWCWIF 420

Query: 421 WSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQEGEIG 480
           WSV+VEI+DLFGSK+IAVRAWDET NTQPENLNWNLMGMMNNSWYRIKINICKPQ+GEIG
Sbjct: 421 WSVDVEILDLFGSKQIAVRAWDETTNTQPENLNWNLMGMMNNSWYRIKINICKPQKGEIG 480

Query: 481 IVFRHPTLAGNQSGGWKEKGKPIPTNSS-SQPSNNKTTTAATTTTFPMSEVKTHNSPESA 540
           IVFRHPT+AGN +GGW+EK KP PT+ S SQ  +NK     +TTTF MSEVKTHNS ESA
Sbjct: 481 IVFRHPTVAGNNAGGWQEKDKPNPTDESPSQQKDNK-----STTTFSMSEVKTHNSSESA 540

Query: 541 WIVVHNQVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSEKAIKMLEDYKIGDLLIL 600
           WIVV N VYDCTPFLK HPGGADSILINAGTDCTEEF+AIHS+KAIKMLEDYKIGDLLIL
Sbjct: 541 WIVVQNHVYDCTPFLKDHPGGADSILINAGTDCTEEFEAIHSDKAIKMLEDYKIGDLLIL 600

Query: 601 NENKDATSSPKLISGHVALVPREKIPVKLISKISISHNTRIFRFGLSSEDQTLGLPVGKH 660
           N+NKDATSSPK ++ HVAL+PREKIPVKLISK +ISHNTRIFRFGLSSEDQ LGLPVGKH
Sbjct: 601 NDNKDATSSPKAVTQHVALIPREKIPVKLISKTTISHNTRIFRFGLSSEDQRLGLPVGKH 660

Query: 661 IFLCANIGGKLCMRAYTPSSAVDQMGYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLPLGS 720
           IFLCAN+ GKLCMRAYTPSSAVDQ GYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLPLGS
Sbjct: 661 IFLCANVDGKLCMRAYTPSSAVDQTGYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLPLGS 720

Query: 721 IVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPEDETEM 780
           +V+VKGPLGHIEYMGRGNF V+GKP+ AKKLAMVAGGTGITPIYQVVQAILKDPEDETEM
Sbjct: 721 MVEVKGPLGHIEYMGRGNFRVNGKPRLAKKLAMVAGGTGITPIYQVVQAILKDPEDETEM 780

Query: 781 FVVYANRTEEDILLREELDTWAKENERLKIWYVVKECVKEGWPYSVGYIKESVLREHIPM 840
           FVVYANR+EEDILLREEL+TWAKENERLKIWYVVK+ V+EGWPYSVGYI+E +LREHIPM
Sbjct: 781 FVVYANRSEEDILLREELETWAKENERLKIWYVVKDGVQEGWPYSVGYIREDILREHIPM 840

Query: 841 GSPETLALVCGPPPMIELTVKPILKDLEYDLESSLLVF 878
           GSP+TLALVCGPPPMIELTVKPILK+LEYDLE SLLVF
Sbjct: 841 GSPQTLALVCGPPPMIELTVKPILKNLEYDLEGSLLVF 873

BLAST of HG10009882 vs. ExPASy TrEMBL
Match: A0A6J1FF98 (Nitrate reductase OS=Cucurbita moschata OX=3662 GN=LOC111444943 PE=3 SV=1)

HSP 1 Score: 1590.5 bits (4117), Expect = 0.0e+00
Identity = 765/882 (86.73%), Postives = 817/882 (92.63%), Query Frame = 0

Query: 1   MAAAVNHKTLDLEPVSNNANFPVQRCNSFRSSSFKFKRQAEKPLII----DDDDSSFDRD 60
           MAA V+HKTLD+EP   N +F  QRCNSFRS SFK KRQ E  L I    DDDDSSFDR 
Sbjct: 1   MAAVVDHKTLDMEP-PTNGSFLAQRCNSFRSPSFKIKRQPEITLTIDDDDDDDDSSFDRH 60

Query: 61  DENSNYFRELIQKGKEEIEPSILDPRDEGTGDSGIMRNPCMVRLTGKHPFNSEPPLDRLM 120
           D+N++YFR+LI+KGKEEIE SILDPRDEGT D+GI+RNP M+RLTGKHPFNSEPPLDRLM
Sbjct: 61  DDNNDYFRDLIRKGKEEIELSILDPRDEGTADNGILRNPSMIRLTGKHPFNSEPPLDRLM 120

Query: 121 RHGFITPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFTLDQLVNDFRSLEFPVTIM 180
           RHGFITPAPLHYVRNHGPVPK D S+WTI++TGL+ENPT+FTL QLVNDFRS+EFPVTI 
Sbjct: 121 RHGFITPAPLHYVRNHGPVPKADSSNWTIEITGLLENPTTFTLHQLVNDFRSIEFPVTIT 180

Query: 181 CSGNRRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGGLYIWMEGAD 240
           CSGNRRKEQNM KQTIGFHWGAAVTSTSLWRGV LREVLKRCGIFSR RG LY+WMEGAD
Sbjct: 181 CSGNRRKEQNMFKQTIGFHWGAAVTSTSLWRGVSLREVLKRCGIFSRHRGALYVWMEGAD 240

Query: 241 DLPGGGGCSYGTSIRREMAMDPARDIIIAYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKW 300
           DLPGGGGCSYGTSIRREMAMDP+RDIIIAY QNGDP+PPDHGFPVRMIVPGFTGGRMVKW
Sbjct: 241 DLPGGGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRMIVPGFTGGRMVKW 300

Query: 301 LKRIIVTNRESDNFYHFMDNRVLPSHVADSELAHAEGWWYKPEYVIYEMNINSVITTPSH 360
           LKRIIVTNRESDN YHFMDNRVLPSHV DSELA+AEGWWYKPEYVIYEMNINSVITTPSH
Sbjct: 301 LKRIIVTNRESDNHYHFMDNRVLPSHV-DSELANAEGWWYKPEYVIYEMNINSVITTPSH 360

Query: 361 DETLPINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYW 420
           DE L INSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYW
Sbjct: 361 DEMLSINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYW 420

Query: 421 CWIFWSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQE 480
           CWIFWSVEVEIV+LFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSW+RIKIN+CKPQ 
Sbjct: 421 CWIFWSVEVEIVELFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWFRIKINVCKPQN 480

Query: 481 GEIGIVFRHPTLAGNQSGGWKEKGKPIPTN-SSSQPSNNKTTTAATTTTFPMSEVKTHNS 540
           GE+GIVFRHPTL GNQSGGWKEK K  PT+  SS+P+N+K  T A + TF  SEVKTHN+
Sbjct: 481 GELGIVFRHPTLPGNQSGGWKEKDKINPTDPPSSKPTNDKNKTTA-SKTFSRSEVKTHNT 540

Query: 541 PESAWIVVHNQVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSEKAIKMLEDYKIGD 600
           PES+WIVVH  VYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHS+KAI ML+DY IGD
Sbjct: 541 PESSWIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAINMLQDYIIGD 600

Query: 601 LLILNENKDATSSPKLISGHVALVPREKIPVKLISKISISHNTRIFRFGLSSEDQTLGLP 660
           LL  N+N++ TSS + +  HVALVP +KIPVKLISKISISHNTRIFRFGLSS+DQ LGLP
Sbjct: 601 LLTENDNEEKTSSSEAVEQHVALVPGKKIPVKLISKISISHNTRIFRFGLSSKDQVLGLP 660

Query: 661 VGKHIFLCANIGGKLCMRAYTPSSAVDQMGYFDLVVKIYFKNVHPDFPNGGLMSQYLDSL 720
           VGKHIFLCA I GKLCMRAYTPSS+VD+MG+FDLVVK+YFKNVHPDFPNGGLMSQYLDSL
Sbjct: 661 VGKHIFLCAIIEGKLCMRAYTPSSSVDEMGFFDLVVKVYFKNVHPDFPNGGLMSQYLDSL 720

Query: 721 PLGSIVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPED 780
           PLGS VDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDP+D
Sbjct: 721 PLGSTVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPQD 780

Query: 781 ETEMFVVYANRTEEDILLREELDTWAKENERLKIWYVVKECVKEGWPYSVGYIKESVLRE 840
           +TEM+VVYANRTEEDILLREELDTWAKENERLKIWYVVKE VKEGWPYSVGYIKESVLRE
Sbjct: 781 KTEMYVVYANRTEEDILLREELDTWAKENERLKIWYVVKEGVKEGWPYSVGYIKESVLRE 840

Query: 841 HIPMGSPETLALVCGPPPMIELTVKPILKDLEYDLESSLLVF 878
           HIPMGSPE+LALVCGPPPMIELTVKPILK++EYDLE+SLLVF
Sbjct: 841 HIPMGSPESLALVCGPPPMIELTVKPILKEMEYDLETSLLVF 879

BLAST of HG10009882 vs. ExPASy TrEMBL
Match: A0A6J1FFD6 (Nitrate reductase OS=Cucurbita moschata OX=3662 GN=LOC111444945 PE=3 SV=1)

HSP 1 Score: 1579.3 bits (4088), Expect = 0.0e+00
Identity = 757/881 (85.93%), Postives = 817/881 (92.74%), Query Frame = 0

Query: 1   MAAAVNHKTLDLEPVSNNANFPVQRCNSFRSSSFKFKRQAEKPLII---DDDDSSFDRDD 60
           MAA V+ K LD+E  S N  FPVQRCNSFRSSSFK KR+ E  L I   DDDDSSFDR+D
Sbjct: 1   MAATVDRKALDVETAS-NGGFPVQRCNSFRSSSFKLKRKPEISLSIDDDDDDDSSFDRND 60

Query: 61  ENSNYFRELIQKGKEEIEPSILDPRDEGTGDSGIMRNPCMVRLTGKHPFNSEPPLDRLMR 120
           EN NY+RELI+KGKEEIEPSILDPRDEGT D+G+MRNP ++RLTGKHP N+EPPLDRLMR
Sbjct: 61  ENVNYYRELIRKGKEEIEPSILDPRDEGTADNGVMRNPSILRLTGKHPLNAEPPLDRLMR 120

Query: 121 HGFITPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFTLDQLVNDFRSLEFPVTIMC 180
           HGFITPAPLHYVRNHGPVPK D S+WT+++TGLV+NPTS+TL QL+NDFRS+EFPVTIMC
Sbjct: 121 HGFITPAPLHYVRNHGPVPKVDSSNWTVEITGLVQNPTSYTLHQLLNDFRSIEFPVTIMC 180

Query: 181 SGNRRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGGLYIWMEGADD 240
           S NRRKE NMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSR RG LYIWMEGADD
Sbjct: 181 SANRRKELNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRHRGALYIWMEGADD 240

Query: 241 LPGGGGCSYGTSIRREMAMDPARDIIIAYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKWL 300
           LPGGGGC+YGTSI REMAMDP+RDIIIAY QNG+P+PPDHGFPVRMIVPGFTGGRMVKWL
Sbjct: 241 LPGGGGCTYGTSISREMAMDPSRDIIIAYMQNGEPIPPDHGFPVRMIVPGFTGGRMVKWL 300

Query: 301 KRIIVTNRESDNFYHFMDNRVLPSHVADSELAHAEGWWYKPEYVIYEMNINSVITTPSHD 360
           KRIIVTNRESDN+YHFMDNRVLPSHV DSELA+AEGWWYKPEYV+YEMNINSVITTPSHD
Sbjct: 301 KRIIVTNRESDNYYHFMDNRVLPSHV-DSELANAEGWWYKPEYVVYEMNINSVITTPSHD 360

Query: 361 ETLPINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWC 420
           ETL I+S+ TQSTYTLRGYA+SGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWC
Sbjct: 361 ETLLISSKKTQSTYTLRGYAFSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWC 420

Query: 421 WIFWSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQEG 480
           WIFWSVEVEIV+LFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSW+RIKIN+CKPQ G
Sbjct: 421 WIFWSVEVEIVELFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWFRIKINVCKPQNG 480

Query: 481 EIGIVFRHPTLAGNQSGGWKEKGKPIPTN-SSSQPSNNKTTTAATTTTFPMSEVKTHNSP 540
           E+GIVFRHPTL G+QSGGWK K K  PT+  SS+P+N+K  T A + TF MSEVKTHN+P
Sbjct: 481 ELGIVFRHPTLPGHQSGGWKGKDKINPTDPPSSKPTNDKNKTTA-SKTFSMSEVKTHNTP 540

Query: 541 ESAWIVVHNQVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSEKAIKMLEDYKIGDL 600
           ES+WIVVH  VYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHS+KAI MLEDY IGDL
Sbjct: 541 ESSWIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAINMLEDYIIGDL 600

Query: 601 LILNENKDATSSPKLISGHVALVPREKIPVKLISKISISHNTRIFRFGLSSEDQTLGLPV 660
           L  N+N++ TSS + +  HVALVP +KIPVKLISKISISHNTRIFRFGLSS+DQ LGLPV
Sbjct: 601 LTENDNEEKTSSSEAVEQHVALVPGKKIPVKLISKISISHNTRIFRFGLSSKDQVLGLPV 660

Query: 661 GKHIFLCANIGGKLCMRAYTPSSAVDQMGYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLP 720
           GKHIFLCA I GKLCMRAYTPSS+VD+M +FDLVVK+YFKNVHPDFPNGGLMSQYLDSLP
Sbjct: 661 GKHIFLCAIIEGKLCMRAYTPSSSVDEMSFFDLVVKVYFKNVHPDFPNGGLMSQYLDSLP 720

Query: 721 LGSIVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPEDE 780
           LGS VDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDP+D+
Sbjct: 721 LGSTVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPQDK 780

Query: 781 TEMFVVYANRTEEDILLREELDTWAKENERLKIWYVVKECVKEGWPYSVGYIKESVLREH 840
           TEM+VVYANRTEEDILLREELDTWAKENERLKIWYVVKE VKEGWPYSVGYIKESVLREH
Sbjct: 781 TEMYVVYANRTEEDILLREELDTWAKENERLKIWYVVKEGVKEGWPYSVGYIKESVLREH 840

Query: 841 IPMGSPETLALVCGPPPMIELTVKPILKDLEYDLESSLLVF 878
           IPMGSPE+LALVCGPPPMIELTVKPILK++EYDLE+SLLVF
Sbjct: 841 IPMGSPESLALVCGPPPMIELTVKPILKEMEYDLEASLLVF 878

BLAST of HG10009882 vs. ExPASy TrEMBL
Match: A0A6J1JZH5 (Nitrate reductase OS=Cucurbita maxima OX=3661 GN=LOC111489277 PE=3 SV=1)

HSP 1 Score: 1573.5 bits (4073), Expect = 0.0e+00
Identity = 753/882 (85.37%), Postives = 814/882 (92.29%), Query Frame = 0

Query: 1   MAAAVNHKTLDLEPVSNNANFPVQRCNSFRSSSFKFKRQAEKPLII----DDDDSSFDRD 60
           MAAAV+HK LDLE    N +F VQRCNSFRS SFK KRQ E  L I    DDDDSS DR 
Sbjct: 1   MAAAVDHKALDLE-TPTNGSFLVQRCNSFRSPSFKIKRQPEITLTIDDDDDDDDSSLDRH 60

Query: 61  DENSNYFRELIQKGKEEIEPSILDPRDEGTGDSGIMRNPCMVRLTGKHPFNSEPPLDRLM 120
           D+N++YF +LI+KGKEEIEPS+LDPRDEGT D+GIMRNP M+RLTGKHPFNSEPPLDRLM
Sbjct: 61  DDNNDYFWDLIRKGKEEIEPSVLDPRDEGTADNGIMRNPSMIRLTGKHPFNSEPPLDRLM 120

Query: 121 RHGFITPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFTLDQLVNDFRSLEFPVTIM 180
           RHGFITPAPLHYVRNHGPVPK D S+WTI++TGL+ENPT+FTL QLVNDFRS+EFPVT+M
Sbjct: 121 RHGFITPAPLHYVRNHGPVPKADSSNWTIEITGLLENPTTFTLHQLVNDFRSIEFPVTVM 180

Query: 181 CSGNRRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGGLYIWMEGAD 240
           CSGNRRKEQNM KQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSR RG LY+WMEGAD
Sbjct: 181 CSGNRRKEQNMFKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRHRGALYVWMEGAD 240

Query: 241 DLPGGGGCSYGTSIRREMAMDPARDIIIAYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKW 300
           DLPGGGGCSYGTSIRREMAMDP+RDIIIAY QNGDP+PPDHGFPVRMIVPGFTGGRMVKW
Sbjct: 241 DLPGGGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRMIVPGFTGGRMVKW 300

Query: 301 LKRIIVTNRESDNFYHFMDNRVLPSHVADSELAHAEGWWYKPEYVIYEMNINSVITTPSH 360
           LKRIIVTNRESDN YHFMDNRVLPSHV DSELA+AEGWWYKPEYVIYEMNINSVITTPSH
Sbjct: 301 LKRIIVTNRESDNHYHFMDNRVLPSHV-DSELANAEGWWYKPEYVIYEMNINSVITTPSH 360

Query: 361 DETLPINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYW 420
           DE L INSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYW
Sbjct: 361 DEMLSINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYW 420

Query: 421 CWIFWSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQE 480
           CWIFWSVEVE+V+LFGSKEIAVRAWDETNNTQPENLNWNL+GMMNNSW+RIKIN+CKPQ 
Sbjct: 421 CWIFWSVEVEVVELFGSKEIAVRAWDETNNTQPENLNWNLVGMMNNSWFRIKINVCKPQN 480

Query: 481 GEIGIVFRHPTLAGNQSGGWKEKGKPIPTN-SSSQPSNNKTTTAATTTTFPMSEVKTHNS 540
           GE+GIVFRHPTL GNQSGGWKEK K  P +  SS+P+N+K  T A + TF MSEVKTHN+
Sbjct: 481 GELGIVFRHPTLPGNQSGGWKEKDKINPPDPPSSKPTNDKNRTTA-SKTFSMSEVKTHNT 540

Query: 541 PESAWIVVHNQVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSEKAIKMLEDYKIGD 600
           PES+WIVVH  VYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHS+KAI MLEDY IGD
Sbjct: 541 PESSWIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAINMLEDYIIGD 600

Query: 601 LLILNENKDATSSPKLISGHVALVPREKIPVKLISKISISHNTRIFRFGLSSEDQTLGLP 660
           LL  N++++ TSS + +  HV LVP ++IPVKLISKISISHNTRIFRFGLSS+D+ LGLP
Sbjct: 601 LLTENDSEEKTSSSEPVEQHVELVPGKRIPVKLISKISISHNTRIFRFGLSSKDRVLGLP 660

Query: 661 VGKHIFLCANIGGKLCMRAYTPSSAVDQMGYFDLVVKIYFKNVHPDFPNGGLMSQYLDSL 720
           VGKHIFLCAN+ GK CMRAYTPSS+VD+MG+FDLVVK+YFKN+HPDFPNGGL+SQYLDSL
Sbjct: 661 VGKHIFLCANMEGKPCMRAYTPSSSVDEMGFFDLVVKVYFKNMHPDFPNGGLLSQYLDSL 720

Query: 721 PLGSIVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPED 780
           PLGS VDVKGPLGHIEYMGRGNFTVHGKPKFA+KLAMVAGGTGITPIYQVVQAILKDPED
Sbjct: 721 PLGSTVDVKGPLGHIEYMGRGNFTVHGKPKFAQKLAMVAGGTGITPIYQVVQAILKDPED 780

Query: 781 ETEMFVVYANRTEEDILLREELDTWAKENERLKIWYVVKECVKEGWPYSVGYIKESVLRE 840
           +TEM+VVYANRTEEDILLREELD WA+ENERLKIWYVV E VKEGWPYSVG+IKESVLRE
Sbjct: 781 KTEMYVVYANRTEEDILLREELDRWAEENERLKIWYVVNEGVKEGWPYSVGHIKESVLRE 840

Query: 841 HIPMGSPETLALVCGPPPMIELTVKPILKDLEYDLESSLLVF 878
           HIPMGSPE LALVCGPPPMI+LTVKPILK++EYDLE+SLLVF
Sbjct: 841 HIPMGSPEILALVCGPPPMIDLTVKPILKEMEYDLETSLLVF 879

BLAST of HG10009882 vs. ExPASy TrEMBL
Match: A0A6J1FK45 (Nitrate reductase OS=Cucurbita moschata OX=3662 GN=LOC111444944 PE=3 SV=1)

HSP 1 Score: 1571.6 bits (4068), Expect = 0.0e+00
Identity = 754/881 (85.58%), Postives = 814/881 (92.40%), Query Frame = 0

Query: 1   MAAAVNHKTLDLEPVSNNANFPVQRCNSFRSSSFKFKRQAEKPLII---DDDDSSFDRDD 60
           MAA V+ K LD+E  S N  FPVQRCNSFRSSSFK KR+ E  L I   DDDDSSFDR+D
Sbjct: 1   MAATVDRKALDVETAS-NGGFPVQRCNSFRSSSFKLKRKPEISLSIDDDDDDDSSFDRND 60

Query: 61  ENSNYFRELIQKGKEEIEPSILDPRDEGTGDSGIMRNPCMVRLTGKHPFNSEPPLDRLMR 120
           EN NY+RELI+KGKEEIEPSILDPRDEGT D+G+MRNP ++RLTGKHP N+EPPLDRLMR
Sbjct: 61  ENVNYYRELIRKGKEEIEPSILDPRDEGTADNGVMRNPSILRLTGKHPLNAEPPLDRLMR 120

Query: 121 HGFITPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFTLDQLVNDFRSLEFPVTIMC 180
           HGFITPAPLHYVRNHGPVPK D S+WT+++TGLV+NPTS+TL QL+NDFRS+EFPVTIMC
Sbjct: 121 HGFITPAPLHYVRNHGPVPKVDSSNWTVEITGLVQNPTSYTLHQLLNDFRSIEFPVTIMC 180

Query: 181 SGNRRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGGLYIWMEGADD 240
           S NRRKE NMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSR RG LYIWMEGADD
Sbjct: 181 SANRRKELNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRHRGALYIWMEGADD 240

Query: 241 LPGGGGCSYGTSIRREMAMDPARDIIIAYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKWL 300
           LPGGGGC+YGTSIRREMAMDP+RDIIIAY QNG+ +PPDHGFPVRMIVPGFTGGRMVKWL
Sbjct: 241 LPGGGGCTYGTSIRREMAMDPSRDIIIAYMQNGEHIPPDHGFPVRMIVPGFTGGRMVKWL 300

Query: 301 KRIIVTNRESDNFYHFMDNRVLPSHVADSELAHAEGWWYKPEYVIYEMNINSVITTPSHD 360
           KRIIVTNRES+N+YHFMDNRVLPSHV DSELA+AEGWWYKPEYV+YEMNINSVITTPSHD
Sbjct: 301 KRIIVTNRESNNYYHFMDNRVLPSHV-DSELANAEGWWYKPEYVVYEMNINSVITTPSHD 360

Query: 361 ETLPINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWC 420
           ETL I+S+ TQSTYTLRGYA+SGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWC
Sbjct: 361 ETLLISSKKTQSTYTLRGYAFSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWC 420

Query: 421 WIFWSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQEG 480
           WIFWSVEVEIV+LFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSW+RIKIN+CKPQ G
Sbjct: 421 WIFWSVEVEIVELFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWFRIKINVCKPQNG 480

Query: 481 EIGIVFRHPTLAGNQSGGWKEKGKPIPTN-SSSQPSNNKTTTAATTTTFPMSEVKTHNSP 540
           E+GIVFRHPTL GNQSGGWKEK K  PT+  SS+P+N+K  T A + TF MSEVKTHN+P
Sbjct: 481 ELGIVFRHPTLPGNQSGGWKEKDKINPTDPPSSKPTNDKNKTTA-SKTFSMSEVKTHNTP 540

Query: 541 ESAWIVVHNQVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSEKAIKMLEDYKIGDL 600
           ES+WIVVH  VYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHS+KAI MLEDY IGDL
Sbjct: 541 ESSWIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAINMLEDYIIGDL 600

Query: 601 LILNENKDATSSPKLISGHVALVPREKIPVKLISKISISHNTRIFRFGLSSEDQTLGLPV 660
           L  N+N++ TSS + +  HVALVP +KIPVKLISKISISHNTRIFRFGLSS+DQ LGLPV
Sbjct: 601 LTENDNEEKTSSSEAVEQHVALVPGKKIPVKLISKISISHNTRIFRFGLSSKDQVLGLPV 660

Query: 661 GKHIFLCANIGGKLCMRAYTPSSAVDQMGYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLP 720
           GKHIFLCA I GKLCMRAYTPSS+VD+ G+FDLVVK+YFKNVHPDFPNGGLMSQYLDSLP
Sbjct: 661 GKHIFLCAIIEGKLCMRAYTPSSSVDETGFFDLVVKVYFKNVHPDFPNGGLMSQYLDSLP 720

Query: 721 LGSIVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPEDE 780
           LGS VDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPED+
Sbjct: 721 LGSTVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPEDK 780

Query: 781 TEMFVVYANRTEEDILLREELDTWAKENERLKIWYVVKECVKEGWPYSVGYIKESVLREH 840
           TEM+VVYANRTEEDILLREELDTWAKENERLKIWYVVK+ VKEGWPYSVG+I ES+LREH
Sbjct: 781 TEMYVVYANRTEEDILLREELDTWAKENERLKIWYVVKDGVKEGWPYSVGHINESILREH 840

Query: 841 IPMGSPETLALVCGPPPMIELTVKPILKDLEYDLESSLLVF 878
           IP GSPE LALVCGPPPMIELTVKPILKDLEY+LE+ LLVF
Sbjct: 841 IPTGSPECLALVCGPPPMIELTVKPILKDLEYELETWLLVF 878

BLAST of HG10009882 vs. TAIR 10
Match: AT1G77760.1 (nitrate reductase 1 )

HSP 1 Score: 1194.9 bits (3090), Expect = 0.0e+00
Identity = 559/855 (65.38%), Postives = 688/855 (80.47%), Query Frame = 0

Query: 49  DDSSFDRDDENSN----YFRELIQKGKEEIEPSILDPRDEGTGDSGIMRNPCMVRLTGKH 108
           DDSS D +DE+ N    Y++EL++K   ++EPSILDPRDE T DS I RN  M+RLTGKH
Sbjct: 65  DDSSSDDEDESHNRNVPYYKELVKKSNSDLEPSILDPRDESTADSWIQRNSSMLRLTGKH 124

Query: 109 PFNSEPPLDRLMRHGFITPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFTLDQLVN 168
           PFN+E PL RLM HGFITP PLHYVRNHG VPK +WS W+I++TGLV+ P  FT+++L++
Sbjct: 125 PFNAEAPLPRLMHHGFITPVPLHYVRNHGAVPKANWSDWSIEITGLVKRPAKFTMEELIS 184

Query: 169 DFRSLEFPVTIMCSGNRRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQ 228
           +F S EFPVT++C+GNRRKEQNMVKQTIGF+WG+A  STSLW+G+PL E+L+RCGI+SR+
Sbjct: 185 EFPSREFPVTLVCAGNRRKEQNMVKQTIGFNWGSAGVSTSLWKGIPLSEILRRCGIYSRR 244

Query: 229 RGGLYIWMEGADDLPGGGGCSYGTSIRREMAMDPARDIIIAYKQNGDPLPPDHGFPVRMI 288
            G L +  EGA+DLPGGGG  YGTSI++EMAMDPARDII+AY QNG+ L PDHGFPVR+I
Sbjct: 245 GGALNVCFEGAEDLPGGGGSKYGTSIKKEMAMDPARDIILAYMQNGELLTPDHGFPVRVI 304

Query: 289 VPGFTGGRMVKWLKRIIVTNRESDNFYHFMDNRVLPSHVADSELAHAEGWWYKPEYVIYE 348
           VPGF GGRMVKWLKRIIVT +ESD++YH+ DNRVLPS + D+ELA++E WWYKPEY+I E
Sbjct: 305 VPGFIGGRMVKWLKRIIVTPQESDSYYHYKDNRVLPS-LVDAELANSEAWWYKPEYIINE 364

Query: 349 MNINSVITTPSHDETLPINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLE 408
           +NINSVITTP H E LPIN+ TTQ  YTL+GYAYSGGGKKVTRV+VTLDGG+TW +C L+
Sbjct: 365 LNINSVITTPGHAEILPINAFTTQKPYTLKGYAYSGGGKKVTRVEVTLDGGDTWSVCELD 424

Query: 409 YHEKPNKYGKYWCWIFWSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSW 468
           + EKPNKYGK+WCW FWS++VE++DL  +K++AVRAWDE+ NTQP+ L WNLMGMMNN W
Sbjct: 425 HQEKPNKYGKFWCWCFWSLDVEVLDLLSAKDVAVRAWDESFNTQPDKLIWNLMGMMNNCW 484

Query: 469 YRIKINICKPQEGEIGIVFRHPTLAGNQSGGWKEKGKPIPTNSSSQPSNNKTTTAATTTT 528
           +RI+ N+CKP  GEIGIVF HPT  GNQSGGW  K + +  +S S  +  K+ ++    T
Sbjct: 485 FRIRTNVCKPHRGEIGIVFEHPTRPGNQSGGWMAKERQLEISSESNNTLKKSVSSPFMNT 544

Query: 529 ----FPMSEVKTHNSPESAWIVVHNQVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIH 588
               + +SEV+ HN+ +SAWI+VH  +YDCT FLK HPGG DSILINAGTDCTEEF+AIH
Sbjct: 545 ASKMYSISEVRKHNTADSAWIIVHGHIYDCTRFLKDHPGGTDSILINAGTDCTEEFEAIH 604

Query: 589 SEKAIKMLEDYKIGDLLILNENKDATSSPKLISG---------------HVALV-PREKI 648
           S+KA K+LEDY+IG+L+    +     S    S                ++ALV PREKI
Sbjct: 605 SDKAKKLLEDYRIGELITTGYDSSPNVSVHGASNFGPLLAPIKELTPQKNIALVNPREKI 664

Query: 649 PVKLISKISISHNTRIFRFGLSSEDQTLGLPVGKHIFLCANIGGKLCMRAYTPSSAVDQM 708
           PV+LI K SISH+ R FRF L SEDQ LGLPVGKH+F+CANI  KLC+RAYTP+SA+D +
Sbjct: 665 PVRLIEKTSISHDVRKFRFALPSEDQQLGLPVGKHVFVCANINDKLCLRAYTPTSAIDAV 724

Query: 709 GYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLPLGSIVDVKGPLGHIEYMGRGNFTVHGKP 768
           G+ DLVVK+YFK+VHP FPNGGLMSQ+LDSLP+GS++D+KGPLGHIEY G+GNF V GKP
Sbjct: 725 GHIDLVVKVYFKDVHPRFPNGGLMSQHLDSLPIGSMIDIKGPLGHIEYKGKGNFLVSGKP 784

Query: 769 KFAKKLAMVAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEEDILLREELDTWA-KE 828
           KFAKKLAM+AGGTGITPIYQ++Q+IL DPEDETEM+VVYANRTE+DIL+REEL+ WA K 
Sbjct: 785 KFAKKLAMLAGGTGITPIYQIIQSILSDPEDETEMYVVYANRTEDDILVREELEGWASKH 844

Query: 829 NERLKIWYVVKECVKEGWPYSVGYIKESVLREHIPMG-SPETLALVCGPPPMIELTVKPI 878
            ERLKIWYVV E  KEGW YS G+I E+VLREHIP G   E+LAL CGPPPMI+  ++P 
Sbjct: 845 KERLKIWYVV-EIAKEGWSYSTGFITEAVLREHIPEGLEGESLALACGPPPMIQFALQPN 904

BLAST of HG10009882 vs. TAIR 10
Match: AT1G37130.1 (nitrate reductase 2 )

HSP 1 Score: 1190.6 bits (3079), Expect = 0.0e+00
Identity = 559/870 (64.25%), Postives = 683/870 (78.51%), Query Frame = 0

Query: 42  KPLIIDDDDSSFDRDDENSN------YFRELIQKGKEEIEPSILDPRDEGTGDSGIMRNP 101
           KP  + DDD     +DEN        Y++E+I+K   E+EPS+LDPRDE T DS I RNP
Sbjct: 50  KPAKVHDDDEDVSSEDENETHNSNAVYYKEMIRKSNAELEPSVLDPRDEYTADSWIERNP 109

Query: 102 CMVRLTGKHPFNSEPPLDRLMRHGFITPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPT 161
            MVRLTGKHPFNSE PL+RLM HGFITP PLHYVRNHG VPK  W+ WT++VTG V+ P 
Sbjct: 110 SMVRLTGKHPFNSEAPLNRLMHHGFITPVPLHYVRNHGHVPKAQWAEWTVEVTGFVKRPM 169

Query: 162 SFTLDQLVNDFRSLEFPVTIMCSGNRRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVL 221
            FT+DQLV++F   EF  T++C+GNRRKEQNMVK++ GF+WG+A  STS+WRGVPL +VL
Sbjct: 170 KFTMDQLVSEFAYREFAATLVCAGNRRKEQNMVKKSKGFNWGSAGVSTSVWRGVPLCDVL 229

Query: 222 KRCGIFSRQRGGLYIWMEGADDLPGGGGCS---YGTSIRREMAMDPARDIIIAYKQNGDP 281
           +RCGIFSR+ G L +  EG++DLPGG G +   YGTSI++E AMDP+RDII+AY QNG+ 
Sbjct: 230 RRCGIFSRKGGALNVCFEGSEDLPGGAGTAGSKYGTSIKKEYAMDPSRDIILAYMQNGEY 289

Query: 282 LPPDHGFPVRMIVPGFTGGRMVKWLKRIIVTNRESDNFYHFMDNRVLPSHVADSELAHAE 341
           L PDHGFPVR+I+PGF GGRMVKWLKRIIVT +ESDNFYHF DNRVLPS + D+ELA  E
Sbjct: 290 LTPDHGFPVRIIIPGFIGGRMVKWLKRIIVTTKESDNFYHFKDNRVLPS-LVDAELADEE 349

Query: 342 GWWYKPEYVIYEMNINSVITTPSHDETLPINSQTTQSTYTLRGYAYSGGGKKVTRVQVTL 401
           GWWYKPEY+I E+NINSVITTP H+E LPIN+ TTQ  YTL+GYAYSGGGKKVTRV+VT+
Sbjct: 350 GWWYKPEYIINELNINSVITTPCHEEILPINAFTTQRPYTLKGYAYSGGGKKVTRVEVTV 409

Query: 402 DGGETWKICNLEYHEKPNKYGKYWCWIFWSVEVEIVDLFGSKEIAVRAWDETNNTQPENL 461
           DGGETW +C L++ EKPNKYGK+WCW FWS+EVE++DL  +KEIAVRAWDET NTQPE +
Sbjct: 410 DGGETWNVCALDHQEKPNKYGKFWCWCFWSLEVEVLDLLSAKEIAVRAWDETLNTQPEKM 469

Query: 462 NWNLMGMMNNSWYRIKINICKPQEGEIGIVFRHPTLAGNQSGGWKEKGKPIPTNSSSQPS 521
            WNLMGMMNN W+R+K N+CKP +GEIGIVF HPTL GN+SGGW  K + +  ++ + PS
Sbjct: 470 IWNLMGMMNNCWFRVKTNVCKPHKGEIGIVFEHPTLPGNESGGWMAKERHLEKSADAPPS 529

Query: 522 NNKTTTA----ATTTTFPMSEVKTHNSPESAWIVVHNQVYDCTPFLKAHPGGADSILINA 581
             K+ +      T   + MSEVK HNS +S WI+VH  +YDCT FL  HPGG+DSILINA
Sbjct: 530 LKKSVSTPFMNTTAKMYSMSEVKKHNSADSCWIIVHGHIYDCTRFLMDHPGGSDSILINA 589

Query: 582 GTDCTEEFDAIHSEKAIKMLEDYKIGDLLILNENKDATSSPKLISG-------------- 641
           GTDCTEEF+AIHS+KA KMLEDY+IG+L+    + D++S    + G              
Sbjct: 590 GTDCTEEFEAIHSDKAKKMLEDYRIGELITTGYSSDSSSPNNSVHGSSAVFSLLAPIGEA 649

Query: 642 ----HVALV-PREKIPVKLISKISISHNTRIFRFGLSSEDQTLGLPVGKHIFLCANIGGK 701
               ++ALV PR K+PV+L+ K SISH+ R FRF L  ED  LGLPVGKHIFLCA I  K
Sbjct: 650 TPVRNLALVNPRAKVPVQLVEKTSISHDVRKFRFALPVEDMVLGLPVGKHIFLCATINDK 709

Query: 702 LCMRAYTPSSAVDQMGYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLPLGSIVDVKGPLGH 761
           LC+RAYTPSS VD +GYF+LVVKIYF  VHP FPNGGLMSQYLDSLP+GS +++KGPLGH
Sbjct: 710 LCLRAYTPSSTVDVVGYFELVVKIYFGGVHPRFPNGGLMSQYLDSLPIGSTLEIKGPLGH 769

Query: 762 IEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEE 821
           +EY+G+G+FTVHGKPKFA KLAM+AGGTGITP+YQ++QAILKDPEDETEM+V+YANRTEE
Sbjct: 770 VEYLGKGSFTVHGKPKFADKLAMLAGGTGITPVYQIIQAILKDPEDETEMYVIYANRTEE 829

Query: 822 DILLREELDTWAKE-NERLKIWYVVKECVKEGWPYSVGYIKESVLREHIPMG-SPETLAL 878
           DILLREELD WA++  +RLK+WYVV E  KEGW YS G+I E+++REHIP G     LA+
Sbjct: 830 DILLREELDGWAEQYPDRLKVWYVV-ESAKEGWAYSTGFISEAIMREHIPDGLDGSALAM 889

BLAST of HG10009882 vs. TAIR 10
Match: AT3G01910.1 (sulfite oxidase )

HSP 1 Score: 212.6 bits (540), Expect = 1.3e-54
Identity = 128/405 (31.60%), Postives = 192/405 (47.41%), Query Frame = 0

Query: 93  RNPCMVRLTGKHPFNSEPPLDRLMRHGFITPAPLHYVRNHGPVPKTD-WSSWTIDVTGLV 152
           R+P + ++  K PFN+EPP   L+   ++TP  L Y RNHGP+P  D   S+++ +TGL+
Sbjct: 16  RHPSL-KVNAKEPFNAEPPRSALV-SSYVTPVDLFYKRNHGPIPIVDHLQSYSVTLTGLI 75

Query: 153 ENPTSFTLDQLVNDFRSL---EFPVTIMCSGNRRKEQNMVKQTIGFHWGAAVTSTSLWRG 212
           +NP        + D RSL       T+ C+GNRR   + V+   G  W  +    ++W G
Sbjct: 76  QNPRKL----FIKDIRSLPKYNVTATLQCAGNRRTAMSKVRNVRGVGWDVSAIGNAVWGG 135

Query: 213 VPLREVLKRCGI----FSRQRGGLYIWMEGADDLPGGGGCSYGTSIRREMAMDPARDIII 272
             L +VL+  GI     S   G  ++     D      G  Y  SI    A +P  D+++
Sbjct: 136 AKLADVLELVGIPKLTASTNLGARHVEFVSVDRCKEENGGPYKASITLSQATNPEADVLL 195

Query: 273 AYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKWLKRIIVTNRESDNFYHFMDNRVLPSHVA 332
           AY+ NG+ L  DHGFP+R++VPG  G R VKWL  I V   ES  F+   D ++ P  V 
Sbjct: 196 AYEMNGETLNRDHGFPLRVVVPGVIGARSVKWLDSINVIAEESQGFFMQKDYKMFPPSV- 255

Query: 333 DSELAHAEGWWYKPEYVIYEMNINSVITTPSHDETLPINS---------QTTQSTYTLRG 392
                    W           NIN     P  D   P+ S                +++G
Sbjct: 256 --------NW----------DNINWSSRRPQMD--FPVQSAICSVEDVQMVKPGKVSIKG 315

Query: 393 YAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKY-------GKYWCWIFWSVEVEIV 452
           YA SGGG+ + RV ++LDGG+ W        ++P K           W W+ +      +
Sbjct: 316 YAVSGGGRGIERVDISLDGGKNW--VEASRTQEPGKQYISEHSSSDKWAWVLFEA---TI 375

Query: 453 DLFGSKEIAVRAWDETNNTQPENLN--WNLMGMMNNSWYRIKINI 472
           D+  + E+  +A D   N QPEN+   WNL G++N SW+R+ + +
Sbjct: 376 DVSQTTEVIAKAVDSAANVQPENVESVWNLRGVLNTSWHRVLLRL 388

BLAST of HG10009882 vs. TAIR 10
Match: AT5G20080.1 (FAD/NAD(P)-binding oxidoreductase )

HSP 1 Score: 167.2 bits (422), Expect = 6.1e-41
Identity = 109/295 (36.95%), Postives = 160/295 (54.24%), Query Frame = 0

Query: 595 DLLILNENKDATSSPKLISGHVALVPREKIPVKLISKISISHNTRIFRFGLSSEDQTLGL 654
           +L+ L++ K+ T  PK      AL P + +  KL     +SHNT++FRF      + LGL
Sbjct: 56  NLVYLDQAKEET-GPK-----TALNPDKWLEFKLQDTARVSHNTQLFRFSFDPSAE-LGL 115

Query: 655 PVGKHIFLCANIG------GKLCMRAYTPSSAVDQMGYFDLVVKIYFKNVHPDFPNGGLM 714
            V   +   A +G       K  +R YTP S  +  GYFDL++K+Y     PD    G M
Sbjct: 116 HVASCLLTRAPLGYNAEGKTKYVIRPYTPISDPEAKGYFDLLIKVY-----PD----GKM 175

Query: 715 SQYLDSLPLGSIVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQA 774
           SQ+  SL  G +++VKGP+   +Y           P   K + M+AGG+GITP+ QV+ A
Sbjct: 176 SQHFASLKPGDVLEVKGPVEKFKY----------SPNMKKHIGMIAGGSGITPMLQVIDA 235

Query: 775 ILKDPEDETEMFVVYANRTEEDILLREELDTWAKENERLKIWYVVKECVKEGWPYSVGYI 834
           I+K+PED T++ ++YAN + +DILL+++LD     +  LKI+Y V    K  W   VGYI
Sbjct: 236 IVKNPEDNTQISLLYANVSPDDILLKQKLDVLQANHPNLKIFYTVDNPTK-NWKGGVGYI 295

Query: 835 KESVLREHIPMGSPETLALVCGPPPMIEL------------TVKPILKDLEYDLE 872
            + +  + +P+ + +TL LVCGPP M+E              VK ILK+L Y  E
Sbjct: 296 SKDMALKGLPLPTDDTLILVCGPPGMMEHISGGKAPDWSQGEVKGILKELGYTEE 323

BLAST of HG10009882 vs. TAIR 10
Match: AT3G01910.2 (sulfite oxidase )

HSP 1 Score: 159.1 bits (401), Expect = 1.7e-38
Identity = 96/319 (30.09%), Postives = 144/319 (45.14%), Query Frame = 0

Query: 175 IMCSGNRRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGI----FSRQRGGLYI 234
           + C+GNRR   + V+   G  W  +    ++W G  L +VL+  GI     S   G  ++
Sbjct: 1   MQCAGNRRTAMSKVRNVRGVGWDVSAIGNAVWGGAKLADVLELVGIPKLTASTNLGARHV 60

Query: 235 WMEGADDLPGGGGCSYGTSIRREMAMDPARDIIIAYKQNGDPLPPDHGFPVRMIVPGFTG 294
                D      G  Y  SI    A +P  D+++AY+ NG+ L  DHGFP+R++VPG  G
Sbjct: 61  EFVSVDRCKEENGGPYKASITLSQATNPEADVLLAYEMNGETLNRDHGFPLRVVVPGVIG 120

Query: 295 GRMVKWLKRIIVTNRESDNFYHFMDNRVLPSHVADSELAHAEGWWYKPEYVIYEMNINSV 354
            R VKWL  I V   ES  F+   D ++ P  V          W           NIN  
Sbjct: 121 ARSVKWLDSINVIAEESQGFFMQKDYKMFPPSV---------NW----------DNINWS 180

Query: 355 ITTPSHDETLPINS---------QTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKIC 414
              P  D   P+ S                +++GYA SGGG+ + RV ++LDGG+ W   
Sbjct: 181 SRRPQMD--FPVQSAICSVEDVQMVKPGKVSIKGYAVSGGGRGIERVDISLDGGKNW--V 240

Query: 415 NLEYHEKPNKY-------GKYWCWIFWSVEVEIVDLFGSKEIAVRAWDETNNTQPENLN- 472
                ++P K           W W+ +      +D+  + E+  +A D   N QPEN+  
Sbjct: 241 EASRTQEPGKQYISEHSSSDKWAWVLFEA---TIDVSQTTEVIAKAVDSAANVQPENVES 293

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038875756.10.0e+0094.87nitrate reductase [NADH]-like [Benincasa hispida][more]
NP_001267688.10.0e+0090.32nitrate reductase [NAD(P)H]-like [Cucumis sativus] >AFH74423.1 cucumber nitrate ... [more]
KAE8652087.10.0e+0089.53hypothetical protein Csa_018542 [Cucumis sativus][more]
XP_023551317.10.0e+0087.07nitrate reductase [NADH]-like [Cucurbita pepo subsp. pepo][more]
KAG6578997.10.0e+0086.65hypothetical protein SDJN03_23445, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
P175690.0e+0065.33Nitrate reductase [NADH] OS=Cucurbita maxima OX=3661 PE=2 SV=1[more]
P277830.0e+0067.24Nitrate reductase [NAD(P)H] OS=Betula pendula OX=3505 GN=NIA1 PE=2 SV=1[more]
P398650.0e+0063.68Nitrate reductase [NADH] 1 OS=Phaseolus vulgaris OX=3885 GN=NIA1 PE=3 SV=1[more]
P085090.0e+0065.26Nitrate reductase [NADH] 2 OS=Nicotiana tabacum OX=4097 GN=NIA2 PE=2 SV=2[more]
P398700.0e+0066.32Inducible nitrate reductase [NADH] 2 OS=Glycine max OX=3847 GN=INR2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
I0CCB00.0e+0090.32Nitrate reductase OS=Cucumis sativus OX=3659 GN=NR3 PE=2 SV=1[more]
A0A6J1FF980.0e+0086.73Nitrate reductase OS=Cucurbita moschata OX=3662 GN=LOC111444943 PE=3 SV=1[more]
A0A6J1FFD60.0e+0085.93Nitrate reductase OS=Cucurbita moschata OX=3662 GN=LOC111444945 PE=3 SV=1[more]
A0A6J1JZH50.0e+0085.37Nitrate reductase OS=Cucurbita maxima OX=3661 GN=LOC111489277 PE=3 SV=1[more]
A0A6J1FK450.0e+0085.58Nitrate reductase OS=Cucurbita moschata OX=3662 GN=LOC111444944 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G77760.10.0e+0065.38nitrate reductase 1 [more]
AT1G37130.10.0e+0064.25nitrate reductase 2 [more]
AT3G01910.11.3e-5431.60sulfite oxidase [more]
AT5G20080.16.1e-4136.95FAD/NAD(P)-binding oxidoreductase [more]
AT3G01910.21.7e-3830.09sulfite oxidase [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008335Eukaryotic molybdopterin oxidoreductasePRINTSPR00407EUMOPTERINcoord: 373..387
score: 77.93
coord: 173..184
score: 70.74
coord: 274..287
score: 75.71
coord: 439..451
score: 63.76
coord: 120..136
score: 80.78
coord: 290..303
score: 83.81
coord: 414..424
score: 60.61
coord: 452..465
score: 63.02
coord: 199..214
score: 54.31
coord: 98..112
score: 82.67
coord: 342..354
score: 66.5
coord: 387..399
score: 67.69
coord: 184..196
score: 71.62
coord: 257..273
score: 63.27
IPR001199Cytochrome b5-like heme/steroid binding domainPRINTSPR00363CYTOCHROMEB5coord: 547..557
score: 64.74
coord: 557..571
score: 69.8
coord: 584..596
score: 50.12
coord: 572..579
score: 64.2
IPR001199Cytochrome b5-like heme/steroid binding domainSMARTSM01117Cyt_b5_2coord: 525..597
e-value: 2.0E-25
score: 100.5
IPR001199Cytochrome b5-like heme/steroid binding domainPFAMPF00173Cyt-b5coord: 527..596
e-value: 1.5E-21
score: 76.3
IPR001199Cytochrome b5-like heme/steroid binding domainPROSITEPS50255CYTOCHROME_B5_2coord: 522..597
score: 25.913258
IPR001709Flavoprotein pyridine nucleotide cytochrome reductasePRINTSPR00371FPNCRcoord: 673..680
score: 54.6
coord: 777..786
score: 37.36
coord: 751..770
score: 48.16
coord: 845..853
score: 45.08
coord: 556..566
score: 34.59
coord: 789..800
score: 47.22
coord: 705..714
score: 35.06
coord: 821..837
score: 25.76
IPR001834NADH:cytochrome b5 reductase-likePRINTSPR00406CYTB5RDTASEcoord: 789..800
score: 69.62
coord: 652..663
score: 75.0
coord: 673..680
score: 71.35
coord: 718..732
score: 65.69
coord: 845..853
score: 75.0
coord: 751..770
score: 72.29
IPR000572Oxidoreductase, molybdopterin-binding domainPFAMPF00174Oxidored_molybcoord: 132..311
e-value: 9.7E-54
score: 181.5
IPR036400Cytochrome b5-like heme/steroid binding domain superfamilyGENE3D3.10.120.10coord: 521..597
e-value: 7.0E-28
score: 98.5
IPR036400Cytochrome b5-like heme/steroid binding domain superfamilySUPERFAMILY55856Cytochrome b5-like heme/steroid binding domaincoord: 521..596
IPR036374Oxidoreductase, molybdopterin-binding domain superfamilyGENE3D3.90.420.10coord: 102..343
e-value: 4.0E-90
score: 303.0
IPR036374Oxidoreductase, molybdopterin-binding domain superfamilySUPERFAMILY56524Oxidoreductase molybdopterin-binding domaincoord: 82..342
NoneNo IPR availableGENE3D2.60.40.650coord: 344..520
e-value: 4.1E-61
score: 207.9
NoneNo IPR availableGENE3D2.40.30.10Translation factorscoord: 613..727
e-value: 6.6E-39
score: 134.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 492..520
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 502..520
NoneNo IPR availablePANTHERPTHR19370:SF198NITRATE REDUCTASEcoord: 58..877
NoneNo IPR availablePANTHERPTHR19370NADH-CYTOCHROME B5 REDUCTASEcoord: 58..877
NoneNo IPR availableCDDcd06183cyt_b5_reduct_likecoord: 626..869
e-value: 1.48956E-100
score: 310.653
NoneNo IPR availableCDDcd02112eukary_NR_Mococoord: 83..469
e-value: 0.0
score: 719.165
IPR005066Moybdenum cofactor oxidoreductase, dimerisationPFAMPF03404Mo-co_dimercoord: 340..470
e-value: 4.7E-53
score: 178.9
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainGENE3D3.40.50.80coord: 728..877
e-value: 7.0E-44
score: 151.2
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainSUPERFAMILY52343Ferredoxin reductase-like, C-terminal NADP-linked domaincoord: 720..871
IPR008333Flavoprotein pyridine nucleotide cytochrome reductase-like, FAD-binding domainPFAMPF00970FAD_binding_6coord: 626..732
e-value: 3.5E-34
score: 117.1
IPR012137Nitrate reductase NADH dependentPIRSFPIRSF000233Nitr_rd_NADHcoord: 13..876
e-value: 0.0
score: 1191.7
IPR001433Oxidoreductase FAD/NAD(P)-bindingPFAMPF00175NAD_binding_1coord: 752..858
e-value: 9.9E-33
score: 113.1
IPR022407Oxidoreductase, molybdopterin binding sitePROSITEPS00559MOLYBDOPTERIN_EUKcoord: 149..184
IPR018506Cytochrome b5, heme-binding sitePROSITEPS00191CYTOCHROME_B5_1coord: 553..560
IPR017927FAD-binding domain, ferredoxin reductase-typePROSITEPS51384FAD_FRcoord: 621..733
score: 14.063556
IPR014756Immunoglobulin E-setSUPERFAMILY81296E set domainscoord: 344..471
IPR017938Riboflavin synthase-like beta-barrelSUPERFAMILY63380Riboflavin synthase domain-likecoord: 616..734

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10009882.1HG10009882.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042128 nitrate assimilation
biological_process GO:0006809 nitric oxide biosynthetic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0071949 FAD binding
molecular_function GO:0020037 heme binding
molecular_function GO:0030151 molybdenum ion binding
molecular_function GO:0043546 molybdopterin cofactor binding
molecular_function GO:0009703 nitrate reductase (NADH) activity
molecular_function GO:0050464 nitrate reductase (NADPH) activity
molecular_function GO:0016491 oxidoreductase activity