HG10009748 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10009748
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	DNA-directed RNA polymerase II subunit RPB7
Location	Chr06: 9702021 .. 9704165 (-)
RNA-Seq Expression	HG10009748
Synteny	HG10009748

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTCTTCCACATAGTTTTGGAGAGGAATATGCAGCTACATCCACGGCACTTTGGCCGTAACCTCCGCGAGAACCTGGTGTCAAAACTAATGAAAGATGTTGAGGGCACTTGCAGGTTTCTTAACTATTTTCCCTACCACATTCCTGTTGCTGTTATTTTCTTTTTCTTAATTTTAGAGCAAAATGCATGTGATAAGATGAAGTGCTAGATTTGATCTTGGTTATACTTATTGACATTGTTAATATGTTTGATAGAGAGGGGTTGGACTTCTGAGAAACATGTGAAAACTGTAAAGATTTCTCAGATTTTTACATTAGTTATTTTAATAAACATCCAACTCTCATTGAAGCAATGAAACTTCCAAGTTCTAATGGGGTGATGAACAGAAGTTCAGATTACTAATTGGGCAATTGATGTTTGTAGTCAAATATTAGGGAAATTTTTTATTTCTCTATACTAGACATGTTTTTAGTTTTTAAATTTTTTATTTGAAATCTTCAAAGAAGCGAGAAATACAAAAAAAAAAAGGTTAGGATGGTATTTGGTCACACTCCATTGATATTAGAATTAGGGTAACTTTTTCTATGAATGTGAGATCTTTACCCCTCAAACCAGGGGAAGACAGTCTCCTCTAACACCATGCACAAAGGCTCAAAATTGCCTTAATGGGATAGAGGGAGCATAACTGATGTCTGATGCTGGTTTAAATTTTGTTTCCTAGCATTTTTTCTGTGCTGCATGGTATCATAGAATTCTATTCTGATGCATATGCATGTCATTTCATCTTCATATAAAGAAAAACATGATCAGTTCATGAACGGATGAAAGCAAATAAAAACAGTTTGATGGATGATATTTCTTTTGAATGTTTGATACTTTTTTCAACTAGTGAACGGTTAGAGGGATGTTGTTATTGATAAAATTTTGAATGTTGACACAGTGGCCGACATGGTTTCGTAGTGGCAATCACGGGAATTGAGAATATCGGGAAAGGGTTGATTCGTGATGGGACAGGTTTCGTCACTTTTCCCGTTAAATACCAGTGTGTTGTGTTCAGACCATTTAAAGGAGAAATTTTAGAAGCAGTTGTTACGATGGTAAATAAGGTATGTATATGTCACTCTTTTGTTATCTAGTAATTAATTGATGAATTTATTTTATAAAATAAATACATTTAATGACTGCAATTGCAACTATTGTAGATGGGGTTCTTTGCTGAAGCTGGACCTGTGCAGATTTTTGTTTCAAACCATGTAAGTATATATTTGTAGTACATTTCAATAATCACCTTTTTTGCTTTCTCTGATATCACACCACCTACTTGAATGTCCTCTCACTAAACTGAATTTGCGACACCAACATCTTGACTTTTATACCTATCATATATTTTCCCCCATTAATGTGCTGAACTTTCATTGAAGTTATCTCACATCTTGTGTTTCAGTTGATACCAGATGATATGGAGTTTCAGTCCGGGGATGTGCCAAACTATACCACTTCTGATGGATCAGTATATTTCTATTTTACATGCATTCTCTAACATAGACTGATAATGCTCTCTTGTTGTGTTAAATTTACTGAATTGTTTATGTTCTCTTGTAGGTTAAGATTCAGAAGGACAGTGAAGTTCGATTGAAGATAATCGGGACTCGAGTAGATGCAACTGAAATTGTAGTGCCTTCTCTTCCTTATTTAGTATTGTTTTTTTCGAAATTTATTTGTTCTGTTCTGTCCTCTGCATGGTGCAATTTTTCTTTTCTATGCAAATATAGTTTTTGGCCTTCATCCTGGGTAACATTTTCCTTTTATCTCAGTTTTGAATTAGGTGATATGTTGATATTTTAATGATAGGAAAGATATAATATATATATATATTCGGCTGAATGAGATTCTTTTTAACTTTTTACATGAAAGTTCAAAAGTTGTGTCCTATTTAGCATATATTCTCTCAAGTGGTGAAAATGTTCAAAACTTGACAACTCCTTGGTAGTTGCTTTTGTGACTTTTCATAGAATCTGTGTGCTATGCTCAAATTCTAGAATGTAATGTATCTCATTTTAACTATTGTTTTTGCTTGCAGTTTTGTATTGGAACCATAAAAGATGACTTCTTGGGTGTGATCAATGACCCTTCAGCGGCCTAG

mRNA sequence

ATGTTCTTCCACATAGTTTTGGAGAGGAATATGCAGCTACATCCACGGCACTTTGGCCGTAACCTCCGCGAGAACCTGGTGTCAAAACTAATGAAAGATGTTGAGGGCACTTGCAGTGGCCGACATGGTTTCGTAGTGGCAATCACGGGAATTGAGAATATCGGGAAAGGGTTGATTCGTGATGGGACAGGTTTCGTCACTTTTCCCGTTAAATACCAGTGTGTTGTGTTCAGACCATTTAAAGGAGAAATTTTAGAAGCAGTTGTTACGATGGTAAATAAGATGGGGTTCTTTGCTGAAGCTGGACCTGTGCAGATTTTTGTTTCAAACCATTTGATACCAGATGATATGGAGTTTCAGTCCGGGGATGTGCCAAACTATACCACTTCTGATGGATCAGTTAAGATTCAGAAGGACAGTGAAGTTCGATTGAAGATAATCGGGACTCGAGTAGATGCAACTGAAATTGTAGTGCCTTCTCTTCCTTATTTATTTTGTATTGGAACCATAAAAGATGACTTCTTGGGTGTGATCAATGACCCTTCAGCGGCCTAG

Coding sequence (CDS)

ATGTTCTTCCACATAGTTTTGGAGAGGAATATGCAGCTACATCCACGGCACTTTGGCCGTAACCTCCGCGAGAACCTGGTGTCAAAACTAATGAAAGATGTTGAGGGCACTTGCAGTGGCCGACATGGTTTCGTAGTGGCAATCACGGGAATTGAGAATATCGGGAAAGGGTTGATTCGTGATGGGACAGGTTTCGTCACTTTTCCCGTTAAATACCAGTGTGTTGTGTTCAGACCATTTAAAGGAGAAATTTTAGAAGCAGTTGTTACGATGGTAAATAAGATGGGGTTCTTTGCTGAAGCTGGACCTGTGCAGATTTTTGTTTCAAACCATTTGATACCAGATGATATGGAGTTTCAGTCCGGGGATGTGCCAAACTATACCACTTCTGATGGATCAGTTAAGATTCAGAAGGACAGTGAAGTTCGATTGAAGATAATCGGGACTCGAGTAGATGCAACTGAAATTGTAGTGCCTTCTCTTCCTTATTTATTTTGTATTGGAACCATAAAAGATGACTTCTTGGGTGTGATCAATGACCCTTCAGCGGCCTAG

Protein sequence

MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQSGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIVVPSLPYLFCIGTIKDDFLGVINDPSAA

Homology

[Close]

BLAST of HG10009748 vs. NCBI nr
Match: XP_004150740.1 (DNA-directed RNA polymerase II subunit RPB7 isoform X1 [Cucumis sativus] >XP_011655125.1 DNA-directed RNA polymerase II subunit RPB7 isoform X1 [Cucumis sativus] >XP_031742310.1 DNA-directed RNA polymerase II subunit RPB7 isoform X1 [Cucumis sativus] >KGN50884.1 hypothetical protein Csa_023096 [Cucumis sativus])

HSP 1 Score: 351.7 bits (901), Expect = 3.9e-93
Identity = 176/184 (95.65%), Postives = 176/184 (95.65%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60
           MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR
Sbjct: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120
           DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120

Query: 121 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIVVPSLPYLFCIGTIKDDFLGVIND 180
           SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI        FCIGTIKDDFLGVIND
Sbjct: 121 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI--------FCIGTIKDDFLGVIND 176

Query: 181 PSAA 185
           PSAA
Sbjct: 181 PSAA 176

[Close]

BLAST of HG10009748 vs. NCBI nr
Match: XP_038906688.1 (DNA-directed RNA polymerase II subunit RPB7 [Benincasa hispida] >XP_038906689.1 DNA-directed RNA polymerase II subunit RPB7 [Benincasa hispida] >XP_038906690.1 DNA-directed RNA polymerase II subunit RPB7 [Benincasa hispida])

HSP 1 Score: 350.5 bits (898), Expect = 8.7e-93
Identity = 175/184 (95.11%), Postives = 176/184 (95.65%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60
           MFFHIVLERNMQLHPRHFGRNLR+NLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR
Sbjct: 1   MFFHIVLERNMQLHPRHFGRNLRDNLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120
           DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120

Query: 121 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIVVPSLPYLFCIGTIKDDFLGVIND 180
           SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI        FCIGTIKDDFLGVIND
Sbjct: 121 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI--------FCIGTIKDDFLGVIND 176

Query: 181 PSAA 185
           PSAA
Sbjct: 181 PSAA 176

[Close]

BLAST of HG10009748 vs. NCBI nr
Match: XP_038906991.1 (DNA-directed RNA polymerase II subunit RPB7-like [Benincasa hispida])

HSP 1 Score: 349.7 bits (896), Expect = 1.5e-92
Identity = 174/184 (94.57%), Postives = 176/184 (95.65%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60
           MFFHIVLERNMQLHPRHFGRNLR+N+VSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR
Sbjct: 1   MFFHIVLERNMQLHPRHFGRNLRDNIVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120
           DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120

Query: 121 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIVVPSLPYLFCIGTIKDDFLGVIND 180
           SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI        FCIGTIKDDFLGVIND
Sbjct: 121 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI--------FCIGTIKDDFLGVIND 176

Query: 181 PSAA 185
           PSAA
Sbjct: 181 PSAA 176

[Close]

BLAST of HG10009748 vs. NCBI nr
Match: KAG7032688.1 (hypothetical protein SDJN02_06738, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 349.4 bits (895), Expect = 1.9e-92
Identity = 174/184 (94.57%), Postives = 176/184 (95.65%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60
           MFFHIVLERNMQLHPRHFGRNLRENLV+KLMKDVEGTCSGRHGFVVAITGIENIGKGLIR
Sbjct: 6   MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 65

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120
           DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ
Sbjct: 66  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 125

Query: 121 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIVVPSLPYLFCIGTIKDDFLGVIND 180
           SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI        FCIGTIKDDFLGVIND
Sbjct: 126 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI--------FCIGTIKDDFLGVIND 181

Query: 181 PSAA 185
           P+AA
Sbjct: 186 PAAA 181

[Close]

BLAST of HG10009748 vs. NCBI nr
Match: XP_022923594.1 (DNA-directed RNA polymerase II subunit RPB7-like [Cucurbita moschata] >XP_022923602.1 DNA-directed RNA polymerase II subunit RPB7-like [Cucurbita moschata] >XP_022990163.1 DNA-directed RNA polymerase II subunit RPB7-like [Cucurbita maxima] >XP_022990164.1 DNA-directed RNA polymerase II subunit RPB7-like [Cucurbita maxima] >XP_023525602.1 DNA-directed RNA polymerase II subunit RPB7-like [Cucurbita pepo subsp. pepo] >KAG6601991.1 hypothetical protein SDJN03_07224, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 349.4 bits (895), Expect = 1.9e-92
Identity = 174/184 (94.57%), Postives = 176/184 (95.65%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60
           MFFHIVLERNMQLHPRHFGRNLRENLV+KLMKDVEGTCSGRHGFVVAITGIENIGKGLIR
Sbjct: 1   MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120
           DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120

Query: 121 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIVVPSLPYLFCIGTIKDDFLGVIND 180
           SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI        FCIGTIKDDFLGVIND
Sbjct: 121 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI--------FCIGTIKDDFLGVIND 176

Query: 181 PSAA 185
           P+AA
Sbjct: 181 PAAA 176

[Close]

BLAST of HG10009748 vs. ExPASy Swiss-Prot
Match: P46279 (DNA-directed RNA polymerase II subunit RPB7 OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 3.1e-93
Identity = 169/182 (92.86%), Postives = 174/182 (95.60%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60
           MFFHIVLERNMQLHPR+FGRNLR+NLVSKLMKDVEGTCSGRHGFVVA+TGIENIGKGLIR
Sbjct: 1   MFFHIVLERNMQLHPRYFGRNLRDNLVSKLMKDVEGTCSGRHGFVVAVTGIENIGKGLIR 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120
           DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120

Query: 121 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIVVPSLPYLFCIGTIKDDFLGVIND 180
           SGD+PNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI        FCIGTIKDDFLGVIND
Sbjct: 121 SGDMPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI--------FCIGTIKDDFLGVIND 174

Query: 181 PS 183
           P+
Sbjct: 181 PA 174

[Close]

BLAST of HG10009748 vs. ExPASy Swiss-Prot
Match: P38421 (DNA-directed RNA polymerase II subunit 7 OS=Arabidopsis thaliana OX=3702 GN=NRPB7 PE=1 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 1.2e-89
Identity = 163/184 (88.59%), Postives = 171/184 (92.93%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60
           MFFHIVLERNMQLHPR FGRNL+ENLVSKLMKDVEGTCSGRHGFVVAITGI+ IGKGLIR
Sbjct: 1   MFFHIVLERNMQLHPRFFGRNLKENLVSKLMKDVEGTCSGRHGFVVAITGIDTIGKGLIR 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120
           DGTGFVTFPVKYQCVVFRPFKGEILEAVVT+VNKMGFFAEAGPVQIFVS HLIPDDMEFQ
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTLVNKMGFFAEAGPVQIFVSKHLIPDDMEFQ 120

Query: 121 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIVVPSLPYLFCIGTIKDDFLGVIND 180
           +GD+PNYTTSDGSVKIQK+ EVRLKIIGTRVDAT I        FC+GTIKDDFLGVIND
Sbjct: 121 AGDMPNYTTSDGSVKIQKECEVRLKIIGTRVDATAI--------FCVGTIKDDFLGVIND 176

Query: 181 PSAA 185
           P+AA
Sbjct: 181 PAAA 176

[Close]

BLAST of HG10009748 vs. ExPASy Swiss-Prot
Match: Q5E9B8 (DNA-directed RNA polymerase II subunit RPB7 OS=Bos taurus OX=9913 GN=POLR2G PE=1 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 8.6e-51
Identity = 94/180 (52.22%), Postives = 130/180 (72.22%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60
           MF+HI LE  + LHPR+FG NL   +  KL  +VEGTC+G++GFV+A+T I+NIG G+I+
Sbjct: 1   MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQ 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120
            G GFV +PVKY+ +VFRPFKGE+++AVVT VNK+G F E GP+  F+S H IP +MEF 
Sbjct: 61  PGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFD 120

Query: 121 -SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIVVPSLPYLFCIGTIKDDFLGVIN 180
            + + P Y T D  + IQ+D E+RLKI+GTRVD  +I        F IG++ DD+LG+++
Sbjct: 121 PNSNPPCYKTMDEDIVIQQDDEIRLKIVGTRVDKNDI--------FAIGSLMDDYLGLVS 172

[Close]

BLAST of HG10009748 vs. ExPASy Swiss-Prot
Match: Q7ZW41 (DNA-directed RNA polymerase II subunit RPB7 OS=Danio rerio OX=7955 GN=polr2g PE=2 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 8.6e-51
Identity = 95/180 (52.78%), Postives = 130/180 (72.22%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60
           MF+HI LE  + LHPR+FG NL   +  KL  +VEGTC+G++GFV+A+T I+NIG G+I+
Sbjct: 1   MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQ 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120
            G GFV +PVKY+ +VFRPFKGE+++AVVT VNK+G F E GP+  F+S H IP +MEF 
Sbjct: 61  PGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFD 120

Query: 121 -SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIVVPSLPYLFCIGTIKDDFLGVIN 180
            + + P Y T D  V IQ+D E+RLKI+GTRVD  +I        F IG++ DD+LG+++
Sbjct: 121 PNSNPPCYKTVDEDVVIQQDDEIRLKIVGTRVDKNDI--------FAIGSLMDDYLGLVS 172

[Close]

BLAST of HG10009748 vs. ExPASy Swiss-Prot
Match: P62487 (DNA-directed RNA polymerase II subunit RPB7 OS=Homo sapiens OX=9606 GN=POLR2G PE=1 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 8.6e-51
Identity = 94/180 (52.22%), Postives = 130/180 (72.22%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60
           MF+HI LE  + LHPR+FG NL   +  KL  +VEGTC+G++GFV+A+T I+NIG G+I+
Sbjct: 1   MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQ 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120
            G GFV +PVKY+ +VFRPFKGE+++AVVT VNK+G F E GP+  F+S H IP +MEF 
Sbjct: 61  PGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFD 120

Query: 121 -SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIVVPSLPYLFCIGTIKDDFLGVIN 180
            + + P Y T D  + IQ+D E+RLKI+GTRVD  +I        F IG++ DD+LG+++
Sbjct: 121 PNSNPPCYKTMDEDIVIQQDDEIRLKIVGTRVDKNDI--------FAIGSLMDDYLGLVS 172

[Close]

BLAST of HG10009748 vs. ExPASy TrEMBL
Match: A0A0A0KPS4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G312340 PE=3 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 1.9e-93
Identity = 176/184 (95.65%), Postives = 176/184 (95.65%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60
           MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR
Sbjct: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120
           DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120

Query: 121 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIVVPSLPYLFCIGTIKDDFLGVIND 180
           SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI        FCIGTIKDDFLGVIND
Sbjct: 121 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI--------FCIGTIKDDFLGVIND 176

Query: 181 PSAA 185
           PSAA
Sbjct: 181 PSAA 176

[Close]

BLAST of HG10009748 vs. ExPASy TrEMBL
Match: A0A6J1JSG6 (DNA-directed RNA polymerase II subunit RPB7-like OS=Cucurbita maxima OX=3661 GN=LOC111487136 PE=3 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 9.4e-93
Identity = 174/184 (94.57%), Postives = 176/184 (95.65%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60
           MFFHIVLERNMQLHPRHFGRNLRENLV+KLMKDVEGTCSGRHGFVVAITGIENIGKGLIR
Sbjct: 1   MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120
           DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120

Query: 121 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIVVPSLPYLFCIGTIKDDFLGVIND 180
           SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI        FCIGTIKDDFLGVIND
Sbjct: 121 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI--------FCIGTIKDDFLGVIND 176

Query: 181 PSAA 185
           P+AA
Sbjct: 181 PAAA 176

[Close]

BLAST of HG10009748 vs. ExPASy TrEMBL
Match: A0A6J1E6V3 (DNA-directed RNA polymerase II subunit RPB7-like OS=Cucurbita moschata OX=3662 GN=LOC111431229 PE=3 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 9.4e-93
Identity = 174/184 (94.57%), Postives = 176/184 (95.65%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60
           MFFHIVLERNMQLHPRHFGRNLRENLV+KLMKDVEGTCSGRHGFVVAITGIENIGKGLIR
Sbjct: 1   MFFHIVLERNMQLHPRHFGRNLRENLVTKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120
           DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120

Query: 121 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIVVPSLPYLFCIGTIKDDFLGVIND 180
           SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI        FCIGTIKDDFLGVIND
Sbjct: 121 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI--------FCIGTIKDDFLGVIND 176

Query: 181 PSAA 185
           P+AA
Sbjct: 181 PAAA 176

[Close]

BLAST of HG10009748 vs. ExPASy TrEMBL
Match: A0A2I4HHD1 (DNA-directed RNA polymerase II subunit RPB7 OS=Juglans regia OX=51240 GN=LOC109017848 PE=3 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 1.2e-92
Identity = 173/184 (94.02%), Postives = 176/184 (95.65%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60
           MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIEN+GKGLIR
Sbjct: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENVGKGLIR 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120
           DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120

Query: 121 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIVVPSLPYLFCIGTIKDDFLGVIND 180
           SGD+PNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI        FCIGTIKDDFLGVIND
Sbjct: 121 SGDMPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI--------FCIGTIKDDFLGVIND 176

Query: 181 PSAA 185
           P+AA
Sbjct: 181 PAAA 176

[Close]

BLAST of HG10009748 vs. ExPASy TrEMBL
Match: A0A6J1CIV0 (DNA-directed RNA polymerase II subunit RPB7 OS=Momordica charantia OX=3673 GN=LOC111012000 PE=3 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 1.6e-92
Identity = 173/184 (94.02%), Postives = 176/184 (95.65%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60
           MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR
Sbjct: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120
           DGTGFVTFPVKYQCVVFRPFKGEILEAVV+MVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVSMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120

Query: 121 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIVVPSLPYLFCIGTIKDDFLGVIND 180
           SGD+PNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI        FCIGTIKDDFLGVIND
Sbjct: 121 SGDIPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEI--------FCIGTIKDDFLGVIND 176

Query: 181 PSAA 185
           P+AA
Sbjct: 181 PAAA 176

[Close]

BLAST of HG10009748 vs. TAIR 10
Match: AT5G59180.1 (DNA-directed RNA polymerase II )

HSP 1 Score: 330.5 bits (846), Expect = 8.7e-91
Identity = 163/184 (88.59%), Postives = 171/184 (92.93%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60
           MFFHIVLERNMQLHPR FGRNL+ENLVSKLMKDVEGTCSGRHGFVVAITGI+ IGKGLIR
Sbjct: 1   MFFHIVLERNMQLHPRFFGRNLKENLVSKLMKDVEGTCSGRHGFVVAITGIDTIGKGLIR 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120
           DGTGFVTFPVKYQCVVFRPFKGEILEAVVT+VNKMGFFAEAGPVQIFVS HLIPDDMEFQ
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTLVNKMGFFAEAGPVQIFVSKHLIPDDMEFQ 120

Query: 121 SGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIVVPSLPYLFCIGTIKDDFLGVIND 180
           +GD+PNYTTSDGSVKIQK+ EVRLKIIGTRVDAT I        FC+GTIKDDFLGVIND
Sbjct: 121 AGDMPNYTTSDGSVKIQKECEVRLKIIGTRVDATAI--------FCVGTIKDDFLGVIND 176

Query: 181 PSAA 185
           P+AA
Sbjct: 181 PAAA 176

[Close]

BLAST of HG10009748 vs. TAIR 10
Match: AT4G14660.1 (RNA polymerase Rpb7-like, N-terminal domain )

HSP 1 Score: 74.7 bits (182), Expect = 8.6e-14
Identity = 44/138 (31.88%), Postives = 76/138 (55.07%), Query Frame = 0


Query: 43  GFVVAITGIENIGKGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAG 102
           G+ VA+T ++ IG+G IR+ TG V FPV +  + F+ FKGEI+  VV  V K G F   G
Sbjct: 47  GYYVAVTTLDKIGEGKIREHTGEVLFPVMFSGMTFKIFKGEIIHGVVHKVLKHGVFMRCG 106

Query: 103 PVQIFVSNHLIPDDMEFQSGDVPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIVVPSLP 162
           P++    ++    D ++  G+ P +     S +IQ ++ VR+ +IG +    E    +L 
Sbjct: 107 PIENVYLSYTKMPDYKYIPGENPIFMNEKTS-RIQVETTVRVVVIGIKWMEVEREFQAL- 166

Query: 163 YLFCIGTIKDDFLGVIND 181
                 +++ D+LG +++
Sbjct: 167 -----ASLEGDYLGPLSE 177

[Close]

BLAST of HG10009748 vs. TAIR 10
Match: AT3G22900.1 (RNA polymerase Rpb7-like, N-terminal domain )

HSP 1 Score: 73.6 bits (179), Expect = 1.9e-13
Identity = 39/107 (36.45%), Postives = 63/107 (58.88%), Query Frame = 0


Query: 43  GFVVAITGIENIGKGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAG 102
           G+++  T +ENIG+G I++ TG + FPV +  + F+ FKGEI+  VV  V+K G F ++G
Sbjct: 46  GYLITPTILENIGEGKIKEQTGEIQFPVVFNGICFKMFKGEIVHGVVHKVHKTGVFLKSG 105

Query: 103 PVQIFVSNHLIPDDMEFQSGDVPNYTTSDGSVKIQKDSEVRLKIIGT 150
           P +I   +H+     EF  G+ P +     S +IQ  + VR  ++ T
Sbjct: 106 PYEIIYLSHMKMPGYEFIPGENPFFMNQYMS-RIQIGARVRFVVLDT 151

[Close]

BLAST of HG10009748 vs. TAIR 10
Match: AT1G06790.2 (RNA polymerase Rpb7 N-terminal domain-containing protein )

HSP 1 Score: 43.9 bits (102), Expect = 1.6e-04
Identity = 26/87 (29.89%), Postives = 42/87 (48.28%), Query Frame = 0


Query: 1  MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60
          MF+   LE ++++ P      L + + S L            G  V+I  I+++  G + 
Sbjct: 1  MFYLSELEHSLRVPPHLLNLPLEDAIKSVLQNVFLDKVLADLGLCVSIYDIKSVEGGFVL 60

Query: 61 DGTGFVTFPVKYQCVVFRPFKGEILEA 88
           G G  T+ V  + VVFRPF GE++ A
Sbjct: 61 PGDGAATYKVGLRIVVFRPFVGEVIAA 87

[Close]

BLAST of HG10009748 vs. TAIR 10
Match: AT1G06790.1 (RNA polymerase Rpb7 N-terminal domain-containing protein )

HSP 1 Score: 43.9 bits (102), Expect = 1.6e-04
Identity = 26/87 (29.89%), Postives = 42/87 (48.28%), Query Frame = 0


Query: 1  MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60
          MF+   LE ++++ P      L + + S L            G  V+I  I+++  G + 
Sbjct: 1  MFYLSELEHSLRVPPHLLNLPLEDAIKSVLQNVFLDKVLADLGLCVSIYDIKSVEGGFVL 60

Query: 61 DGTGFVTFPVKYQCVVFRPFKGEILEA 88
           G G  T+ V  + VVFRPF GE++ A
Sbjct: 61 PGDGAATYKVGLRIVVFRPFVGEVIAA 87

The following BLAST results are available for this feature:

BLAST of HG10009748 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004150740.1	3.9e-93	95.65	DNA-directed RNA polymerase II subunit RPB7 isoform X1 [Cucumis sativus] >XP_011...	[more]
XP_038906688.1	8.7e-93	95.11	DNA-directed RNA polymerase II subunit RPB7 [Benincasa hispida] >XP_038906689.1 ...	[more]
XP_038906991.1	1.5e-92	94.57	DNA-directed RNA polymerase II subunit RPB7-like [Benincasa hispida]	[more]
KAG7032688.1	1.9e-92	94.57	hypothetical protein SDJN02_06738, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022923594.1	1.9e-92	94.57	DNA-directed RNA polymerase II subunit RPB7-like [Cucurbita moschata] >XP_022923...	[more]

BLAST of HG10009748 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P46279	3.1e-93	92.86	DNA-directed RNA polymerase II subunit RPB7 OS=Glycine max OX=3847 PE=2 SV=1	[more]
P38421	1.2e-89	88.59	DNA-directed RNA polymerase II subunit 7 OS=Arabidopsis thaliana OX=3702 GN=NRPB...	[more]
Q5E9B8	8.6e-51	52.22	DNA-directed RNA polymerase II subunit RPB7 OS=Bos taurus OX=9913 GN=POLR2G PE=1...	[more]
Q7ZW41	8.6e-51	52.78	DNA-directed RNA polymerase II subunit RPB7 OS=Danio rerio OX=7955 GN=polr2g PE=...	[more]
P62487	8.6e-51	52.22	DNA-directed RNA polymerase II subunit RPB7 OS=Homo sapiens OX=9606 GN=POLR2G PE...	[more]

BLAST of HG10009748 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KPS4	1.9e-93	95.65	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G312340 PE=3 SV=1	[more]
A0A6J1JSG6	9.4e-93	94.57	DNA-directed RNA polymerase II subunit RPB7-like OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1E6V3	9.4e-93	94.57	DNA-directed RNA polymerase II subunit RPB7-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A2I4HHD1	1.2e-92	94.02	DNA-directed RNA polymerase II subunit RPB7 OS=Juglans regia OX=51240 GN=LOC1090...	[more]
A0A6J1CIV0	1.6e-92	94.02	DNA-directed RNA polymerase II subunit RPB7 OS=Momordica charantia OX=3673 GN=LO...	[more]

BLAST of HG10009748 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59180.1	8.7e-91	88.59	DNA-directed RNA polymerase II	[more]
AT4G14660.1	8.6e-14	31.88	RNA polymerase Rpb7-like, N-terminal domain	[more]
AT3G22900.1	1.9e-13	36.45	RNA polymerase Rpb7-like, N-terminal domain	[more]
AT1G06790.2	1.6e-04	29.89	RNA polymerase Rpb7 N-terminal domain-containing protein	[more]
AT1G06790.1	1.6e-04	29.89	RNA polymerase Rpb7 N-terminal domain-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036898	RNA polymerase Rpb7-like, N-terminal domain superfamily	GENE3D	3.30.1490.120		coord: 1..80 e-value: 3.6E-28 score: 99.7
IPR036898	RNA polymerase Rpb7-like, N-terminal domain superfamily	SUPERFAMILY	88798	N-terminal, heterodimerisation domain of RBP7 (RpoE)	coord: 1..78
IPR005576	RNA polymerase Rpb7-like , N-terminal	PFAM	PF03876	SHS2_Rpb7-N	coord: 8..64 e-value: 6.1E-13 score: 48.8
None	No IPR available	GENE3D	2.40.50.140		coord: 81..179 e-value: 1.1E-34 score: 120.2
None	No IPR available	PANTHER	PTHR12709:SF8	BNAA10G12180D PROTEIN	coord: 1..184
None	No IPR available	CDD	cd04462	S1_RNAPII_Rpb7	coord: 81..176 e-value: 5.85836E-43 score: 136.622
None	No IPR available	CDD	cd04329	RNAP_II_Rpb7_N	coord: 2..81 e-value: 3.26319E-40 score: 129.224
IPR003029	S1 domain	PFAM	PF00575	S1	coord: 78..157 e-value: 1.0E-16 score: 61.0
IPR045113	RNA polymerase Rpb7-like	PANTHER	PTHR12709	DNA-DIRECTED RNA POLYMERASE II, III	coord: 1..184
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 78..158

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10009748.1	HG10009748.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000291	nuclear-transcribed mRNA catabolic process, exonucleolytic
biological_process	GO:0060213	positive regulation of nuclear-transcribed mRNA poly(A) tail shortening
biological_process	GO:0045948	positive regulation of translational initiation
biological_process	GO:0006367	transcription initiation from RNA polymerase II promoter
biological_process	GO:0006352	DNA-templated transcription, initiation
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0000932	P-body
cellular_component	GO:0005665	RNA polymerase II, core complex
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0003697	single-stranded DNA binding
molecular_function	GO:0003727	single-stranded RNA binding
molecular_function	GO:0031369	translation initiation factor binding
molecular_function	GO:0003676	nucleic acid binding