HG10009694 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10009694
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptiontranslation initiation factor eIF-2B subunit beta-like
LocationChr06: 8969498 .. 8975438 (+)
RNA-Seq ExpressionHG10009694
SyntenyHG10009694
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCAGACGTGCAAGCCCTTGTGAATGATTTCGTTATCAAGCTTAAGAGGCGGTTAGTTCTTTGGAATTGTTGATATTTCTGGAAATTGTGTTGTAGCCTGTGTGCTTTTTGAATATTTTTTTTTATAGTTCGTTTTGTGATGATATTGTGGCAGTAAAATTGAGGGCTCCTTGGCCACCGCGAAGCATACGGCTGAATTGCTTCGGTCTGTCATTTCACAGCAGCGAATACCCTACACGAATCAGGCGGCTGCTTTGATTGATGCTGTGAAAGCTGTTGGCGAGCAGCTGATTGCTGCGAATCCTGTTGGTAGATATATTAATTTTTTTTATTATAGCTTAAAACCCCAGTCTGTCTGTTTGGGATTTTACCCTTTACAAAATTCAACTTATTTCCATGGCTGCTTTTTATTTAGGCTGAAAATGAGTCGTTGATCACCTCTCAATTATCATTATTCGGTCATGGCCATGTTGTTTTATACACTGTCTTTACGTGGTCTATAGCCTTATTCTTTGAATGACTCGCTACTTCGCTCGTCTTAAATCTACTAATTTATTAGCTTTTCACTGGCATTTATATGTTGATATACAACTGCAGAGCTTGCTGTGGGCAATATTGTGAGGCGGGTTTTGCACATTATTAGAGAGGAGGATCTCTCACTCACCACAGCTTCTATTGCTGGGTTGAGCTTATCGGCAGTTAGTGATGATGAAGATGACATTGAAAAAGATGATCGCCCGGTCCTCTCAGCTGCTGCTGTTGCAGCTGCTGCAAGAAGCACTTTGCGGCCACCTTCCTTGCAAACTCTTCTTGAGGATGTTCCTAATCAAACATCGGTTCATCAAACTTCATCATCTGGAGGTGATTCAGAAGGAAAAAGCAAATGTGAGCTTCCTTGTTATATGGTGATTAGAGTTTATTTGCGATTACTTAATAAGGTTTATGTGGTGCTTGAAGGCATTTGTTTGTGATGTCTAAAATATTTACTGATTTGTCTGATTAAAATTGATTACGGGGTTGTAATGGAGGGTGTTGTCTTTTTGGTTGCGATTCTTAGATGTGAATATTTGGAGGTCCAGTTGAATAGATGTGAAGGAAGATTATGATTGATGAGGATTAATTTATTTTCTAATAAGCACATGAATGGTGAGATTATGAAAGCAGTAGCCTCTTCAGGGAATTAGTGATAACACACAAGGGAACCCTTGGCACCTGGGGATCTTTTCTACCGGAAATTATCACCTCCATCTTTATTTATTTCCCTTTCTCTCTTGATTTTTGTTGTAGGGTCCTTGATAATTTTGATTGCTTTCTACTTCTCTTGAACAAAAATCTATCCAGAAATGTGCATGTGTGTTTCAACTTTATCCTATTTTTTTTCATGTGTTCTTTTCTTATGTGAAGTGGTTCTCTGTTTATTCAATTATTTATTATAATCATTATTTTTTTCTATTTGTTTTTTTAACCAGAAACAACTTCTCATTGATTCAATGAAAAATGAAAATGATTAAATGTTCAAGGGATACAAACTCCCAGAGAGAGTGAAAAAGGAAAAAAAATATAAACCTGTAGAGATTAGCTAGGAAAATAGATAAACATTTAAAGAACACAAAACTTTTCCAATATCTTCGTTTGAAAGACTTAGGAAGCTTGCGAATGAATGATGAAACTATGAATAAGGTATTGTTTTTTTGCACTTGGACTGCAAGTTTGCTCTTTTCCAACAAGTTGTTCTCATTGACAGCTGCTGATAAAAGTTCAAGGAGTCGGAAGCTCAAGCATGATGTAATTGAAGCTGTTAATGAGCTTATTCAGGATATTGCAACTTGTCATGAACAGATTGCTGAGCAAGCAGTGGAGCACATTCATCAGAAGTATTTCTCACGTTAACTAATTCTAATAAGCTAAGCTTTTTATTATTATTATTATTTTTTTGTATCCTTAAAATCATATTGATCTGAGCCATCTAATTTATGACTTACTGAAGCAGTAAAGGGTTATTAACTTTGGGAATAGATTTACTGTATATTATTTTATCGTACTCAAGATGTCCTTATGCAAATACCTTCATCTGTTGTCTTCATGTGCTCTATTTCCTTTTTGGTAAGTTGGTCTATGCCTCTTATATCAAAGGCTTGGGTCATGTTGGAGGGGACGTCTGCAGCTTTCGCTACTAATTAAAAAAGTGTTCTAATTTTTTTAATATGGGAAACCAAGTGATAAAACCGAAGTTTCTTGAGAGATTAGGCAAGTAGAATATGCTCCCATTTCCTTTTTGTCCTTGCGACAAACTATTTTAATAGATTTATGTGGAGACATCCACATGAGCATACAGAATGAGCATTCATTGGATGACCAAAGATCGAAAGAAGCTGCCCTTACCTATCTTTAAAACTCTAAAAGGGCCTGTCACTTAGTTTGTGCCTTCTTGGCTTAGGCTTTCAAATGAACTGACTTTATGGCCTTGTCACCTGACGCTATGCTCAACTGGTTAAAAGGCATTCGCATTATATCCTCGAGAAAAAGATTAGATTTGTATCTCCTCTTGTTGCTCAACTCAAAAATAGATTTCTTATGAGGTTTATTATTGGTGGCTTCAAGGTGGGAAAAGCAGCCTATTAGAGAAAATTCTTTTATCTTCCACTAGAATAATTCCTCAATCTCTTAAAATAATTATCTTCAAATTATTATTGGGTTTGAATGTGAATACGAACAAATAAAAGTATTTGAGTATTAATTGTGAAGCTATTCAAGTTCAAGAGCTGGCTTCGATTGTGGGGGTATGGTTTTGACTCTTGGTTTAACCCAGACTTTGGTCTTCCTCTAAATGATCACCCAAAGAACATCCAGTTTTGGGTTCCAATGACTCATATTATTCAAAGGAAGTTGTTAATCAGGAAAAGTTACTCTCTCTAAAGGTGATGGGTTAACTCTCACTCGAGCTTCTCACTCAACCTTTCTTCTTGTCCCCACTTTTTCATGCCCAAAAAGATGGCCCTCATCAATGTAGAATCTATGAGATATTTTTACTGGCAAGGCCGATAAGGTAGGAGGTTCCCACCTTGGTTTGCTGGTAAATGGTCAGGTAACCGATTTCTTTTGGTGCTTTTGATCTTGGAAACCTCTGAATTGTCTTAACGTCTATGGTGATTTGCTTGAGAGAAGCACCTCTGTGGAGGAAAGTGGTGGCTGCTAAATGTAGAGTCTCCAAAGGTGATGATGTTAGGTATCTACCCTGCACTCAGTAAACCCCAACAATATATATAGAAGAAAGAGATGATAATGGATTGCTCTGATATAGTATTTGATTTAAATATAGTCTCAACAACAGTAACAAACAAATCAAATACTCATGCTGTAATAAAGCTATGAAAAGAATACTCAGAAACAGAGAAATAGACAATCTCCCAGCACATTCGAGAGGGCTGGTAGTCTCTCCTTCCTTCCCTAAATAGGGCCTGCTTCAAGTACTCTCTGCTGTCCTCCCTTACCTCAGCAATTCCTTATTTATATCCTTCACAGAGGTCCCCTCCACTACTAACTAACTTCCCTTCAATTCTTCTTTCCATTTCCATCCTCCTGTGAAATCTCCTTCTTACCCTTCCTCTTATAAGTAAATATAATTGGGGGCCTAACAGATGATTGGTTGACTAATCAAATGGATATCAAAGGCTTTCAAGGTTCTTGGAAATAATTTGAATGGTGGCGTACAAGGTGCACCTACCATTTACCTCAGGGACACACCTAGTATTCCAGGTTTCCCAATGCAAGGCTTGGAAACTTGCTGCCCAACCACTTACCTGCTCAACTATTCTAAGCTTGAACTCTTCGTCACATGGCTTGGAGGTTTTGATTTAGTGGGCTGGACAGCAACTTGAGGAATCTCATCAATAATCATTCCCTGCTTTTACTCCTTGAGGGCAAGGTGAAAGTTTGGTGGGTGGGAGGGAATGATAGAAAGCTCAGACCATGGATGGGTCATTTGTTAGGAGGAGAGATGTAGAATGAGTGAGTAGGGTGATGTAATGGTGTATGAATAAGGATAGGGGTTTTATTGTGTAAAAATGTTGAGGTTGTGGGTCTGAGGGAATATATAGTAGGAGGAGCGAGTAATGTGTTAGGCACTCAACATTTTGTTGATCTTGTTCAGATGTCCTTTTTCTTTTCTTGTTATTATTAAATTAGCTTTATTCTGTGTTATATTGCGTGTATTTGATTTACTTGCATCATTTTCTGTTTCTGGCTTCAATATGAAATATGCAATTAGAAGTTAGAGGACTTTCGTATTATGGAATTCTAATTTTAAACGTCCTCTTTAAGAATTTACCTCAATATCCTCACATATATTGAATGCCAAAAATTTTCAGCGAGGTAATATTAACTTTGGGGAGTTCCAGAACAGTATTGGAATTTCTTTGTGCTGCTAAGGAGAAGAAAAGGTCCTTCCGGGTGTTTGTTGCTGAGGGTGCTCCAAGGTCTGATCTAATAGCTAAAGCTAGCTGTCTGTTAGGGTGCAGATTTATATTTGTTTAAGTATTAATAAAAGATTAAGGACATTTTTGGAAGTTATTTTAAAATAGTTAAAATCATTTTTCTTATTTTTAAAATCACTTCGAAACATATATATATATATATATATATATATATATTTATTTAAATCAATTTTTAATAGAATGAAAATCTTGTTTAAAAATGTAAAATCAAATATTAAATTGATTTAGAATGATCAAAGACATGTTTTGAAGTGATTTTGAATCTAACAAAACTGATTTTAACCAATTTAAAATCACGTTGCTTTTGGGTTATTTTACACTTAGATATTTTTGGTTCACTCTTCCTCTTGTTTCTTCCTCTTGTTACAACAGATATCAGGGTCATCTTCTTGCTAAGGAGTTGGTTACAAGAGGGTTACAGACCACACTTATTACTGATTCTGCAATTTTTGCCATGATTTCCCGGGTGAATATGGTAGGTGGTAGCTCTATATTGTGTGTATACATACCTAAATGCATTTTGTTTCAATCTATGCCTAAAGTGGGATGGACATTACAACTGATGAGATGGTGTCATCTTCAAATCTGTTCTGCAAGGTAATAGTTGGAGCTCATGCTGTCATGGCAAATGGTGGTGTCATGGCACCAGTCGGATTGAATATGGTGGCTCTTGCAGCTAAAAGGCATGCTGTTCCTTTTGTCGTGCTAGCTGGAAGTCACAAGGTGCTGTTCCTTTTCACGAAATTTTCTTCTATTATAATTCTACCCAAAATTGTAGGAAAATAACTTATTGGTATTATTTTTCTAAATGTCTGTTACTTATTTAATAGTTATGCCCTTTGTATCCTCACAATCCCGAAGTTCTACTCAATGAACTGAGGTCCCCATCAGAGCTACTGGACTTTGGTGAATTTTCAGATTGCATGGACTTTGGAACTAACTCTGGTTCTCCTCTTCTCCATGTAGTCAACCCTACATTTGACTATGTGCCACCATCACTTGTTAGTTTATTTATTACTGACACGTAAGTCCAGCTCCTTCCTTAAGTAGTTTCAAAAGATTTTCTTTTTGTGTTGTCGAATTTCATTCCTTTTACCTCCAACTCCTGGATAAAAAGGAATTGCATGGGAATAATTATTGGTGGCGTTTCTTATTTATTTATTTATTTATTTTTAGATTCAATTACTACATTTTAAGCAAGATACTTGGGTTTACTTTTCTACTAAACATTTGGATTTCAATTCTGGGTTTCATTTGATATTTTTCCTTAGGAAAAAGAAAAGGAATGCTGTTATATTGATCTTGTTTATGTCTGTTGTTTCTCATTTTTTACTCCTCACATTTGACAGAGGTGGCCATAACTCATCATACATGTATCGGCTTATTGCTGATTACTATTCAGCCGATGATTTGGTAATTAAAAGAAGGCCTTCTACAGGCAGCTGA

mRNA sequence

ATGCCAGACGTGCAAGCCCTTGTGAATGATTTCGTTATCAAGCTTAAGAGGCGTAAAATTGAGGGCTCCTTGGCCACCGCGAAGCATACGGCTGAATTGCTTCGGTCTGTCATTTCACAGCAGCGAATACCCTACACGAATCAGGCGGCTGCTTTGATTGATGCTGTGAAAGCTGTTGGCGAGCAGCTGATTGCTGCGAATCCTGTTGAGCTTGCTGTGGGCAATATTGTGAGGCGGGTTTTGCACATTATTAGAGAGGAGGATCTCTCACTCACCACAGCTTCTATTGCTGGGTTGAGCTTATCGGCAGTTAGTGATGATGAAGATGACATTGAAAAAGATGATCGCCCGGTCCTCTCAGCTGCTGCTGTTGCAGCTGCTGCAAGAAGCACTTTGCGGCCACCTTCCTTGCAAACTCTTCTTGAGGATGTTCCTAATCAAACATCGGTTCATCAAACTTCATCATCTGGAGGTGATTCAGAAGGAAAAAGCAAATCTGCTGATAAAAGTTCAAGGAGTCGGAAGCTCAAGCATGATGTAATTGAAGCTGTTAATGAGCTTATTCAGGATATTGCAACTTGTCATGAACAGATTGCTGAGCAAGCAGTGGAGCACATTCATCAGAACGAGGTAATATTAACTTTGGGGAGTTCCAGAACAGTATTGGAATTTCTTTGTGCTGCTAAGGAGAAGAAAAGGTCCTTCCGGGTGTTTGTTGCTGAGGGTGCTCCAAGATATCAGGGTCATCTTCTTGCTAAGGAGTTGGTTACAAGAGGGTTACAGACCACACTTATTACTGATTCTGCAATTTTTGCCATGATTTCCCGGGTGAATATGGTAATAGTTGGAGCTCATGCTGTCATGGCAAATGGTGGTGTCATGGCACCAGTCGGATTGAATATGGTGGCTCTTGCAGCTAAAAGGCATGCTGTTCCTTTTGTCGTGCTAGCTGGAAGTCACAAGTTATGCCCTTTGTATCCTCACAATCCCGAAGTTCTACTCAATGAACTGAGGTCCCCATCAGAGCTACTGGACTTTGGTGAATTTTCAGATTGCATGGACTTTGGAACTAACTCTGGTTCTCCTCTTCTCCATGTAGTCAACCCTACATTTGACTATGTGCCACCATCACTTGTTAGTTTATTTATTACTGACACAGGTGGCCATAACTCATCATACATGTATCGGCTTATTGCTGATTACTATTCAGCCGATGATTTGGTAATTAAAAGAAGGCCTTCTACAGGCAGCTGA

Coding sequence (CDS)

ATGCCAGACGTGCAAGCCCTTGTGAATGATTTCGTTATCAAGCTTAAGAGGCGTAAAATTGAGGGCTCCTTGGCCACCGCGAAGCATACGGCTGAATTGCTTCGGTCTGTCATTTCACAGCAGCGAATACCCTACACGAATCAGGCGGCTGCTTTGATTGATGCTGTGAAAGCTGTTGGCGAGCAGCTGATTGCTGCGAATCCTGTTGAGCTTGCTGTGGGCAATATTGTGAGGCGGGTTTTGCACATTATTAGAGAGGAGGATCTCTCACTCACCACAGCTTCTATTGCTGGGTTGAGCTTATCGGCAGTTAGTGATGATGAAGATGACATTGAAAAAGATGATCGCCCGGTCCTCTCAGCTGCTGCTGTTGCAGCTGCTGCAAGAAGCACTTTGCGGCCACCTTCCTTGCAAACTCTTCTTGAGGATGTTCCTAATCAAACATCGGTTCATCAAACTTCATCATCTGGAGGTGATTCAGAAGGAAAAAGCAAATCTGCTGATAAAAGTTCAAGGAGTCGGAAGCTCAAGCATGATGTAATTGAAGCTGTTAATGAGCTTATTCAGGATATTGCAACTTGTCATGAACAGATTGCTGAGCAAGCAGTGGAGCACATTCATCAGAACGAGGTAATATTAACTTTGGGGAGTTCCAGAACAGTATTGGAATTTCTTTGTGCTGCTAAGGAGAAGAAAAGGTCCTTCCGGGTGTTTGTTGCTGAGGGTGCTCCAAGATATCAGGGTCATCTTCTTGCTAAGGAGTTGGTTACAAGAGGGTTACAGACCACACTTATTACTGATTCTGCAATTTTTGCCATGATTTCCCGGGTGAATATGGTAATAGTTGGAGCTCATGCTGTCATGGCAAATGGTGGTGTCATGGCACCAGTCGGATTGAATATGGTGGCTCTTGCAGCTAAAAGGCATGCTGTTCCTTTTGTCGTGCTAGCTGGAAGTCACAAGTTATGCCCTTTGTATCCTCACAATCCCGAAGTTCTACTCAATGAACTGAGGTCCCCATCAGAGCTACTGGACTTTGGTGAATTTTCAGATTGCATGGACTTTGGAACTAACTCTGGTTCTCCTCTTCTCCATGTAGTCAACCCTACATTTGACTATGTGCCACCATCACTTGTTAGTTTATTTATTACTGACACAGGTGGCCATAACTCATCATACATGTATCGGCTTATTGCTGATTACTATTCAGCCGATGATTTGGTAATTAAAAGAAGGCCTTCTACAGGCAGCTGA

Protein sequence

MPDVQALVNDFVIKLKRRKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALIDAVKAVGEQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDDIEKDDRPVLSAAAVAAAARSTLRPPSLQTLLEDVPNQTSVHQTSSSGGDSEGKSKSADKSSRSRKLKHDVIEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVAEGAPRYQGHLLAKELVTRGLQTTLITDSAIFAMISRVNMVIVGAHAVMANGGVMAPVGLNMVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTNSGSPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVIKRRPSTGS
Homology
BLAST of HG10009694 vs. NCBI nr
Match: XP_038874367.1 (translation initiation factor eIF-2B subunit beta [Benincasa hispida] >XP_038874368.1 translation initiation factor eIF-2B subunit beta [Benincasa hispida])

HSP 1 Score: 780.0 bits (2013), Expect = 1.0e-221
Identity = 412/417 (98.80%), Postives = 415/417 (99.52%), Query Frame = 0

Query: 1   MPDVQALVNDFVIKLKRRKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALIDAVKAVG 60
           MPDVQALVNDFVIKLKRRKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALI+AVKAVG
Sbjct: 1   MPDVQALVNDFVIKLKRRKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALIEAVKAVG 60

Query: 61  EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDDIEKDDRPVLS 120
           EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDD EKDDRPVLS
Sbjct: 61  EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDDSEKDDRPVLS 120

Query: 121 AAAVAAAARSTLRPPSLQTLLEDVPNQTSVHQTSSSGGDSEGKSKSADKSSRSRKLKHDV 180
           AAAVAAAARSTLRPPSLQTLLEDVPNQTSVHQTSSSGGDSEGKSKS DKSSRSRKLKHDV
Sbjct: 121 AAAVAAAARSTLRPPSLQTLLEDVPNQTSVHQTSSSGGDSEGKSKSVDKSSRSRKLKHDV 180

Query: 181 IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA 240
           IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA
Sbjct: 181 IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA 240

Query: 241 EGAPRYQGHLLAKELVTRGLQTTLITDSAIFAMISRVNMVIVGAHAVMANGGVMAPVGLN 300
           EGAPRYQGHLLAKELVTRGLQTTLITDSA+FAMISRVNMVIVGAHAVMANGGVMAPVGLN
Sbjct: 241 EGAPRYQGHLLAKELVTRGLQTTLITDSAVFAMISRVNMVIVGAHAVMANGGVMAPVGLN 300

Query: 301 MVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTNSG 360
           MVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTN+G
Sbjct: 301 MVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTNTG 360

Query: 361 SPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVIKRRPSTGS 418
           SPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVIKRRPSTGS
Sbjct: 361 SPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVIKRRPSTGS 417

BLAST of HG10009694 vs. NCBI nr
Match: XP_022141443.1 (translation initiation factor eIF-2B subunit beta-like [Momordica charantia])

HSP 1 Score: 773.5 bits (1996), Expect = 9.4e-220
Identity = 407/417 (97.60%), Postives = 414/417 (99.28%), Query Frame = 0

Query: 1   MPDVQALVNDFVIKLKRRKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALIDAVKAVG 60
           MPDVQA+VNDFVIKLK+RKIEGSLATAK TAELLRSVISQQRIPYTNQAAALIDAVKAVG
Sbjct: 1   MPDVQAVVNDFVIKLKKRKIEGSLATAKLTAELLRSVISQQRIPYTNQAAALIDAVKAVG 60

Query: 61  EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDDIEKDDRPVLS 120
           EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTA+IAGLSLSAVSDDEDDIEKDDRP LS
Sbjct: 61  EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTAAIAGLSLSAVSDDEDDIEKDDRPALS 120

Query: 121 AAAVAAAARSTLRPPSLQTLLEDVPNQTSVHQTSSSGGDSEGKSKSADKSSRSRKLKHDV 180
           AAAVAAAARSTLRPPSLQTLLEDVPNQTS+HQTSSSGGDSEGKSKSADKSSRSRKLKHDV
Sbjct: 121 AAAVAAAARSTLRPPSLQTLLEDVPNQTSIHQTSSSGGDSEGKSKSADKSSRSRKLKHDV 180

Query: 181 IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA 240
           IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA
Sbjct: 181 IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA 240

Query: 241 EGAPRYQGHLLAKELVTRGLQTTLITDSAIFAMISRVNMVIVGAHAVMANGGVMAPVGLN 300
           EGAPRYQGHLLAKELVTRGLQTTLITDSA+FAMISRVNMVIVGAHAVMANGGVMAPVGLN
Sbjct: 241 EGAPRYQGHLLAKELVTRGLQTTLITDSAVFAMISRVNMVIVGAHAVMANGGVMAPVGLN 300

Query: 301 MVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTNSG 360
           MVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTN+G
Sbjct: 301 MVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTNTG 360

Query: 361 SPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVIKRRPSTGS 418
           SPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLV+KRRP TGS
Sbjct: 361 SPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVVKRRPPTGS 417

BLAST of HG10009694 vs. NCBI nr
Match: XP_022939685.1 (translation initiation factor eIF-2B subunit beta-like [Cucurbita moschata])

HSP 1 Score: 769.6 bits (1986), Expect = 1.4e-218
Identity = 406/417 (97.36%), Postives = 411/417 (98.56%), Query Frame = 0

Query: 1   MPDVQALVNDFVIKLKRRKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALIDAVKAVG 60
           MPDVQALVNDFVIKLK+RKIEGSLATAKHTAELLRSVIS QRIPYTNQAAALIDAVKAVG
Sbjct: 1   MPDVQALVNDFVIKLKKRKIEGSLATAKHTAELLRSVISHQRIPYTNQAAALIDAVKAVG 60

Query: 61  EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDDIEKDDRPVLS 120
           EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDD EKDD P LS
Sbjct: 61  EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDDSEKDDHPALS 120

Query: 121 AAAVAAAARSTLRPPSLQTLLEDVPNQTSVHQTSSSGGDSEGKSKSADKSSRSRKLKHDV 180
           AAAVAAAARSTLRPPSLQTLLEDVPNQ SVHQTSSSGGDSEGKSKSADKSSRSRKLKHDV
Sbjct: 121 AAAVAAAARSTLRPPSLQTLLEDVPNQASVHQTSSSGGDSEGKSKSADKSSRSRKLKHDV 180

Query: 181 IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA 240
           IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA
Sbjct: 181 IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA 240

Query: 241 EGAPRYQGHLLAKELVTRGLQTTLITDSAIFAMISRVNMVIVGAHAVMANGGVMAPVGLN 300
           EGAPRYQGHLLAKELVTRGLQTTLITDSA+FAMISRVNMVIVGAHAVMANGGVMAPVGLN
Sbjct: 241 EGAPRYQGHLLAKELVTRGLQTTLITDSAVFAMISRVNMVIVGAHAVMANGGVMAPVGLN 300

Query: 301 MVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTNSG 360
           MVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFG+ +G
Sbjct: 301 MVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGSKTG 360

Query: 361 SPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVIKRRPSTGS 418
           SPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVIK+RPSTGS
Sbjct: 361 SPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVIKKRPSTGS 417

BLAST of HG10009694 vs. NCBI nr
Match: XP_023551232.1 (translation initiation factor eIF-2B subunit beta-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 768.8 bits (1984), Expect = 2.3e-218
Identity = 406/417 (97.36%), Postives = 411/417 (98.56%), Query Frame = 0

Query: 1   MPDVQALVNDFVIKLKRRKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALIDAVKAVG 60
           MPDVQALVNDFVIKLK+RKIEGSLATAKHTAELLRSVIS QRIPYTNQAAALIDAVKAVG
Sbjct: 1   MPDVQALVNDFVIKLKKRKIEGSLATAKHTAELLRSVISHQRIPYTNQAAALIDAVKAVG 60

Query: 61  EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDDIEKDDRPVLS 120
           EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDD EKDD P LS
Sbjct: 61  EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDDGEKDDHPALS 120

Query: 121 AAAVAAAARSTLRPPSLQTLLEDVPNQTSVHQTSSSGGDSEGKSKSADKSSRSRKLKHDV 180
           AAAVAAAARSTLRPPSLQTLLEDVPNQ SVHQTSSSGGDSEGKSKSADKSSRSRKLKHDV
Sbjct: 121 AAAVAAAARSTLRPPSLQTLLEDVPNQASVHQTSSSGGDSEGKSKSADKSSRSRKLKHDV 180

Query: 181 IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA 240
           IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA
Sbjct: 181 IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA 240

Query: 241 EGAPRYQGHLLAKELVTRGLQTTLITDSAIFAMISRVNMVIVGAHAVMANGGVMAPVGLN 300
           EGAPRYQGHLLAKELVTRGLQTTLITDSA+FAMISRVNMVIVGAHAVMANGGVMAPVGLN
Sbjct: 241 EGAPRYQGHLLAKELVTRGLQTTLITDSAVFAMISRVNMVIVGAHAVMANGGVMAPVGLN 300

Query: 301 MVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTNSG 360
           MVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFG+ +G
Sbjct: 301 MVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGSKTG 360

Query: 361 SPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVIKRRPSTGS 418
           SPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVIK+RPSTGS
Sbjct: 361 SPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVIKKRPSTGS 417

BLAST of HG10009694 vs. NCBI nr
Match: XP_022923286.1 (translation initiation factor eIF-2B subunit beta-like [Cucurbita moschata] >XP_023535169.1 translation initiation factor eIF-2B subunit beta-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 768.5 bits (1983), Expect = 3.0e-218
Identity = 405/417 (97.12%), Postives = 414/417 (99.28%), Query Frame = 0

Query: 1   MPDVQALVNDFVIKLKRRKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALIDAVKAVG 60
           MPDVQALVNDFVIKLK+RKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALIDAVKAVG
Sbjct: 1   MPDVQALVNDFVIKLKKRKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALIDAVKAVG 60

Query: 61  EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDDIEKDDRPVLS 120
           EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGL+L+AVSDDEDD EKDDRPVLS
Sbjct: 61  EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLNLAAVSDDEDDNEKDDRPVLS 120

Query: 121 AAAVAAAARSTLRPPSLQTLLEDVPNQTSVHQTSSSGGDSEGKSKSADKSSRSRKLKHDV 180
           AAAVAAAARSTLRPPSLQTLLEDVPNQ SVHQTSSSGGDSEGKSKSADKSSRSRKLKHDV
Sbjct: 121 AAAVAAAARSTLRPPSLQTLLEDVPNQASVHQTSSSGGDSEGKSKSADKSSRSRKLKHDV 180

Query: 181 IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA 240
           IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA
Sbjct: 181 IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA 240

Query: 241 EGAPRYQGHLLAKELVTRGLQTTLITDSAIFAMISRVNMVIVGAHAVMANGGVMAPVGLN 300
           EGAPRYQGHLLAKELVTRGLQTTLITDSA+FAMISRVNMVIVGAHAVMANGGV+APVGLN
Sbjct: 241 EGAPRYQGHLLAKELVTRGLQTTLITDSAVFAMISRVNMVIVGAHAVMANGGVIAPVGLN 300

Query: 301 MVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTNSG 360
           M+ALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTN+G
Sbjct: 301 MLALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTNTG 360

Query: 361 SPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVIKRRPSTGS 418
           SPLLHVVNPTFDYVPP+LVSLFITDTGGHNSSYMYRLI+DYYSADDLVIKRR STGS
Sbjct: 361 SPLLHVVNPTFDYVPPALVSLFITDTGGHNSSYMYRLISDYYSADDLVIKRRLSTGS 417

BLAST of HG10009694 vs. ExPASy Swiss-Prot
Match: Q54EY2 (Translation initiation factor eIF-2B subunit beta OS=Dictyostelium discoideum OX=44689 GN=eif2b2 PE=3 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 7.1e-69
Identity = 168/401 (41.90%), Postives = 235/401 (58.60%), Query Frame = 0

Query: 8   VNDFVIKLKRRKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALIDAVKAVGEQLIAAN 67
           +  F++ LKR+ + GS   ++ +AELLR  + + +      A +LID +K +G++L+ A 
Sbjct: 13  LESFILHLKRKTVVGSYQVSRASAELLRQWVHKGK---WGNAKSLIDQIKIIGKKLMNAQ 72

Query: 68  PVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDDIEKDDRPVLSAAAVAAA 127
           P+E  +GNIVRRVL IIREE L+       GL       + DD + DD    ++      
Sbjct: 73  PLEFCIGNIVRRVLFIIREEYLTFFRNKKNGL-------NNDDYDDDDFETTTSNNNNNN 132

Query: 128 ARSTLRPPSLQTLLEDVPNQTSVHQTSSSGGDSEGKSKSADKSSRSRKLKHDVIEAVNEL 187
             + +   S         N+ + +       D     +  D +    +LK  +++++NEL
Sbjct: 133 NNNNINSSS-------NINKNNKYSNVMLQDDPIDDQEDIDFTETFPRLKAAIMDSINEL 192

Query: 188 IQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVAEGAPRYQ 247
           I ++   H  +AEQA+EHIH NE I+TLG SRTV EFL  A  +KRSF+V V E AP  +
Sbjct: 193 IDELEGLHRNVAEQAIEHIHSNETIMTLGCSRTVEEFLKEA-ARKRSFKVIVVETAPSLE 252

Query: 248 GHLLAKELVTRGLQTTLITDSAIFAMISRVNMVIVGAHAVMANGGVMAPVGLNMVALAAK 307
           G   A  L    + TTLITDSA+FAM+SRVN VI+G HAVMANGG++A  G + +A+AAK
Sbjct: 253 GQKTAISLSKASIDTTLITDSAVFAMMSRVNKVIIGTHAVMANGGLIATSGTHTLAVAAK 312

Query: 308 RHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTNSGSPLLHVV 367
            H+VP VV  G +KLCPLY ++ +   N   SP E L F E     +F  N     +H  
Sbjct: 313 YHSVPIVVCTGLYKLCPLYAYDQDT-FNNFGSPGEYLKFEE----AEFLEN-----VHSY 372

Query: 368 NPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLV 409
           NPTFDYV P LVSLFIT+ GGHN SY+YRL+ +YY A D++
Sbjct: 373 NPTFDYVAPDLVSLFITNIGGHNPSYIYRLLQEYYDARDIL 385

BLAST of HG10009694 vs. ExPASy Swiss-Prot
Match: Q9UT76 (Probable translation initiation factor eIF-2B subunit beta OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tif222 PE=1 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.0e-59
Identity = 151/410 (36.83%), Postives = 230/410 (56.10%), Query Frame = 0

Query: 8   VNDFVIKLKRRKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALIDAVKAVGEQLIAAN 67
           V+  +  LK RK++G  A A  TA ++R VISQ R    +Q   LID V+AVG  L+ A 
Sbjct: 13  VSSLIADLKSRKVQGPFAVAVETALVMRQVISQTRWSTVDQ---LIDTVRAVGSTLVKAQ 72

Query: 68  PVELAVGNIVRRVLHIIREEDLSLTTAS------IAGLSLSAVSDDEDDIEKDDRPVLSA 127
           P E + GNI+RR+L +IREE   L   +      I   S S+    + DI  +++ V S 
Sbjct: 73  PTEFSCGNIIRRILRLIREEYQELLKTADENEKLIVSSSNSSSPSQKRDIPSNEKLVQSH 132

Query: 128 AAVAAAARSTLRPPSLQTLLEDVPNQTSVHQTSSSGGDSEGKSKSADKSSRSRKLKHDVI 187
             V+    S++     +  LE   +  +V  +  +GG                 ++  +I
Sbjct: 133 EPVSVQMYSSMLNLLGRPTLESPTHSKTVGDSRVTGG---------------MDMRAVII 192

Query: 188 EAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVAE 247
             + ++I ++   +  I  Q+++H+H NE+ILT G S+TV  FL  A  KKR F V VAE
Sbjct: 193 SGIQDVIDELDKINTDIEVQSMDHLHSNEIILTQGCSKTVEAFLRFA-AKKRKFSVIVAE 252

Query: 248 GAPRYQ--GHLLAKELVTRGLQTTLITDSAIFAMISRVNMVIVGAHAVMANGGVMAPVGL 307
           G P  Q   H +AK L   G+ TT+I+D+ IFA++SRVN VI+G HA++ NGG++   G 
Sbjct: 253 GFPNNQKGSHAMAKRLAQAGIDTTVISDATIFAIMSRVNKVILGTHAILGNGGLVTYSGA 312

Query: 308 NMVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTNS 367
            +VA AA+ HA P VV +G +KL P+YP++ E ++ +L SP +++ F E  D +      
Sbjct: 313 QLVAQAARHHATPVVVCSGIYKLSPVYPYDLESII-QLSSPDKIMSFNE-GDLIS----- 372

Query: 368 GSPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVI 410
                 ++NP +DY+PP LV LFIT+ GG+  SY+YR++ D Y A D ++
Sbjct: 373 ---RAEILNPYYDYIPPDLVDLFITNLGGYPPSYLYRIMNDTYDASDTIL 393

BLAST of HG10009694 vs. ExPASy Swiss-Prot
Match: Q90511 (Translation initiation factor eIF-2B subunit beta OS=Takifugu rubripes OX=31033 GN=eif2b2 PE=3 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 6.9e-56
Identity = 160/412 (38.83%), Postives = 220/412 (53.40%), Query Frame = 0

Query: 3   DVQALVNDFVIKLKR-----RKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALIDAVK 62
           D+   +  F+  LKR       + GS  TA+ T  LLR + +Q R    + A  L++ ++
Sbjct: 9   DLTERIEAFLSDLKRGGSGTGPLRGSSETARETTALLRRITAQAR---WSSAGDLMEIIR 68

Query: 63  AVGEQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDDIEKDDRP 122
             G +LIAA P E  VGN++RRVL IIREE            + S  S +E D       
Sbjct: 69  KEGRRLIAAQPSETTVGNMIRRVLKIIREE-----------YARSRGSSEEAD------- 128

Query: 123 VLSAAAVAAAARSTLRPPSLQTLLEDVPNQTSVHQTSSSGGDSEGKSKSADKSSRSRKLK 182
                                        Q S+H+  +SGG SE   +          L+
Sbjct: 129 ----------------------------QQESLHKLLTSGGLSEENFR-----QHFAALR 188

Query: 183 HDVIEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRV 242
            +VIEA+NEL+ ++    + IA QA+EHIH NEVI+T+G SRTV  FL  A  +KR F V
Sbjct: 189 ANVIEAINELLTELEGTTDNIAMQALEHIHSNEVIMTVGRSRTVEAFLKDA-ARKRKFHV 248

Query: 243 FVAEGAPRYQGHLLAKELVTRGLQTTLITDSAIFAMISRVNMVIVGAHAVMANGGVMAPV 302
            VAE AP  QGH +A  L T G++TT+I D+AIFA++SRVN VI+G   V+ANGG+ A  
Sbjct: 249 IVAECAPFCQGHKMATSLSTAGIETTVIADAAIFAVMSRVNKVIIGTQTVLANGGLRAVN 308

Query: 303 GLNMVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGT 362
           G + +ALAAK H+ P +V A   KL P +P N E   ++  SP E+L F E         
Sbjct: 309 GTHTLALAAKHHSTPLIVCAPMFKLSPQFP-NEEDTFHKFVSPHEVLPFTE--------- 352

Query: 363 NSGSPLLHVVN---PTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADD 407
                +L  VN   P FDYVPP L++LFI++ GGH  SY+YRL+++ Y  +D
Sbjct: 369 ---GEILSKVNVHCPVFDYVPPELITLFISNIGGHAPSYIYRLMSELYHPED 352

BLAST of HG10009694 vs. ExPASy Swiss-Prot
Match: Q5E9B4 (Translation initiation factor eIF-2B subunit beta OS=Bos taurus OX=9913 GN=EIF2B2 PE=2 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 1.9e-53
Identity = 156/403 (38.71%), Postives = 217/403 (53.85%), Query Frame = 0

Query: 8   VNDFVIKLKR-RKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALIDAVKAVGEQLIAA 67
           +  FV  LKR      S   A+ T  LLR +I+  R    + A  L++ ++  G ++ AA
Sbjct: 15  IESFVEALKRGGGRRSSEDMARETLGLLRRIITDHR---WSNAGELMELIRREGRRMTAA 74

Query: 68  NPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDDIEKDDRPVLSAAAVAA 127
            P E  VGN+VRRVL IIREE   L   S           DE D                
Sbjct: 75  QPSETTVGNMVRRVLRIIREEYGRLHGRS-----------DESD---------------- 134

Query: 128 AARSTLRPPSLQTLLEDVPNQTSVHQTSSSGGDSEGKSKSADKSSRSRKLKHDVIEAVNE 187
                               Q S+H+  +SGG SE      D  S   +L+ ++IEA+NE
Sbjct: 135 -------------------QQESLHKLLTSGGLSE------DFRSHYAQLQSNIIEAINE 194

Query: 188 LIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVAEGAPRY 247
           L+ ++    E IA QA+EHIH NEVI+T+G SRTV  FL  A  +KR F V VAE AP  
Sbjct: 195 LLVELEGTTENIAAQALEHIHSNEVIMTIGFSRTVEAFLREA-ARKRKFHVIVAECAPFC 254

Query: 248 QGHLLAKELVTRGLQTTLITDSAIFAMISRVNMVIVGAHAVMANGGVMAPVGLNMVALAA 307
           QGH +A  L   G++TT++TD+AIFA++SRVN VI+G   ++ANG + A  G + +ALAA
Sbjct: 255 QGHEMAVNLSKAGIETTVMTDAAIFAVMSRVNKVIIGTKTILANGALRAVAGTHTLALAA 314

Query: 308 KRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTNSGSPLLHV 367
           K H+ P +V A   KL P +P N E   ++  +P E+L F E  D ++         + V
Sbjct: 315 KHHSTPLIVCAPMFKLSPQFP-NEEDSFHKFVAPEEVLPFTE-GDILE--------KVSV 351

Query: 368 VNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVI 410
             P FDYVPP L++LFI++ GG+  SY+YRL+++ Y  DD V+
Sbjct: 375 HCPVFDYVPPELITLFISNIGGNAPSYIYRLMSELYHPDDHVL 351

BLAST of HG10009694 vs. ExPASy Swiss-Prot
Match: Q99LD9 (Translation initiation factor eIF-2B subunit beta OS=Mus musculus OX=10090 GN=Eif2b2 PE=1 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 3.2e-53
Identity = 157/403 (38.96%), Postives = 217/403 (53.85%), Query Frame = 0

Query: 8   VNDFVIKLKRRKIE-GSLATAKHTAELLRSVISQQRIPYTNQAAALIDAVKAVGEQLIAA 67
           +  FV  LKR   +  S   A+ T  LLR +I+     + N A  L+D ++  G ++ AA
Sbjct: 15  IEGFVETLKRGGGQRSSEDMARETLGLLRRLITDH---HWNNAGDLMDLIRREGRRMTAA 74

Query: 68  NPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDDIEKDDRPVLSAAAVAA 127
            P E  VGN+VRRVL IIREE   L   S           DE D                
Sbjct: 75  QPSETTVGNMVRRVLKIIREEYGRLHGRS-----------DESD---------------- 134

Query: 128 AARSTLRPPSLQTLLEDVPNQTSVHQTSSSGGDSEGKSKSADKSSRSRKLKHDVIEAVNE 187
                               Q S+H+  +SGG SE      D S     LK ++IEA+NE
Sbjct: 135 -------------------QQESLHKLLTSGGLSE------DFSFHFAPLKANIIEAINE 194

Query: 188 LIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVAEGAPRY 247
           L+ ++    E IA QA+EHIH NEVI+T+G SRTV  FL  A  +KR F V VAE AP  
Sbjct: 195 LLVELEGTMENIAAQALEHIHSNEVIMTIGYSRTVEAFLKEA-ARKRKFHVIVAECAPFC 254

Query: 248 QGHLLAKELVTRGLQTTLITDSAIFAMISRVNMVIVGAHAVMANGGVMAPVGLNMVALAA 307
           QGH +A  L   G++TT++TD+AIFA++SRVN VI+G   ++ANG + A  G + +ALAA
Sbjct: 255 QGHEMAVNLSKEGIETTVMTDAAIFAVMSRVNKVIIGTKTILANGSLRAVAGTHTLALAA 314

Query: 308 KRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTNSGSPLLHV 367
           K H+ P +V A   KL P +P + E   ++  +P E+L F E  D ++         + V
Sbjct: 315 KHHSTPLIVCAPMFKLSPQFP-SEEDSFHKFVAPEEVLPFTE-GDILE--------KVSV 351

Query: 368 VNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVI 410
             P FDYVPP L++LFI++ GG+  SY+YRL+++ Y  DD V+
Sbjct: 375 HCPVFDYVPPDLITLFISNIGGNAPSYIYRLMSELYHPDDHVL 351

BLAST of HG10009694 vs. ExPASy TrEMBL
Match: A0A6J1CKI1 (translation initiation factor eIF-2B subunit beta-like OS=Momordica charantia OX=3673 GN=LOC111011838 PE=3 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 4.6e-220
Identity = 407/417 (97.60%), Postives = 414/417 (99.28%), Query Frame = 0

Query: 1   MPDVQALVNDFVIKLKRRKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALIDAVKAVG 60
           MPDVQA+VNDFVIKLK+RKIEGSLATAK TAELLRSVISQQRIPYTNQAAALIDAVKAVG
Sbjct: 1   MPDVQAVVNDFVIKLKKRKIEGSLATAKLTAELLRSVISQQRIPYTNQAAALIDAVKAVG 60

Query: 61  EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDDIEKDDRPVLS 120
           EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTA+IAGLSLSAVSDDEDDIEKDDRP LS
Sbjct: 61  EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTAAIAGLSLSAVSDDEDDIEKDDRPALS 120

Query: 121 AAAVAAAARSTLRPPSLQTLLEDVPNQTSVHQTSSSGGDSEGKSKSADKSSRSRKLKHDV 180
           AAAVAAAARSTLRPPSLQTLLEDVPNQTS+HQTSSSGGDSEGKSKSADKSSRSRKLKHDV
Sbjct: 121 AAAVAAAARSTLRPPSLQTLLEDVPNQTSIHQTSSSGGDSEGKSKSADKSSRSRKLKHDV 180

Query: 181 IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA 240
           IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA
Sbjct: 181 IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA 240

Query: 241 EGAPRYQGHLLAKELVTRGLQTTLITDSAIFAMISRVNMVIVGAHAVMANGGVMAPVGLN 300
           EGAPRYQGHLLAKELVTRGLQTTLITDSA+FAMISRVNMVIVGAHAVMANGGVMAPVGLN
Sbjct: 241 EGAPRYQGHLLAKELVTRGLQTTLITDSAVFAMISRVNMVIVGAHAVMANGGVMAPVGLN 300

Query: 301 MVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTNSG 360
           MVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTN+G
Sbjct: 301 MVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTNTG 360

Query: 361 SPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVIKRRPSTGS 418
           SPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLV+KRRP TGS
Sbjct: 361 SPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVVKRRPPTGS 417

BLAST of HG10009694 vs. ExPASy TrEMBL
Match: A0A6J1FNF3 (translation initiation factor eIF-2B subunit beta-like OS=Cucurbita moschata OX=3662 GN=LOC111445501 PE=3 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 6.6e-219
Identity = 406/417 (97.36%), Postives = 411/417 (98.56%), Query Frame = 0

Query: 1   MPDVQALVNDFVIKLKRRKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALIDAVKAVG 60
           MPDVQALVNDFVIKLK+RKIEGSLATAKHTAELLRSVIS QRIPYTNQAAALIDAVKAVG
Sbjct: 1   MPDVQALVNDFVIKLKKRKIEGSLATAKHTAELLRSVISHQRIPYTNQAAALIDAVKAVG 60

Query: 61  EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDDIEKDDRPVLS 120
           EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDD EKDD P LS
Sbjct: 61  EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDDSEKDDHPALS 120

Query: 121 AAAVAAAARSTLRPPSLQTLLEDVPNQTSVHQTSSSGGDSEGKSKSADKSSRSRKLKHDV 180
           AAAVAAAARSTLRPPSLQTLLEDVPNQ SVHQTSSSGGDSEGKSKSADKSSRSRKLKHDV
Sbjct: 121 AAAVAAAARSTLRPPSLQTLLEDVPNQASVHQTSSSGGDSEGKSKSADKSSRSRKLKHDV 180

Query: 181 IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA 240
           IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA
Sbjct: 181 IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA 240

Query: 241 EGAPRYQGHLLAKELVTRGLQTTLITDSAIFAMISRVNMVIVGAHAVMANGGVMAPVGLN 300
           EGAPRYQGHLLAKELVTRGLQTTLITDSA+FAMISRVNMVIVGAHAVMANGGVMAPVGLN
Sbjct: 241 EGAPRYQGHLLAKELVTRGLQTTLITDSAVFAMISRVNMVIVGAHAVMANGGVMAPVGLN 300

Query: 301 MVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTNSG 360
           MVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFG+ +G
Sbjct: 301 MVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGSKTG 360

Query: 361 SPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVIKRRPSTGS 418
           SPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVIK+RPSTGS
Sbjct: 361 SPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVIKKRPSTGS 417

BLAST of HG10009694 vs. ExPASy TrEMBL
Match: A0A6J1E6E7 (translation initiation factor eIF-2B subunit beta-like OS=Cucurbita moschata OX=3662 GN=LOC111431025 PE=3 SV=1)

HSP 1 Score: 768.5 bits (1983), Expect = 1.5e-218
Identity = 405/417 (97.12%), Postives = 414/417 (99.28%), Query Frame = 0

Query: 1   MPDVQALVNDFVIKLKRRKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALIDAVKAVG 60
           MPDVQALVNDFVIKLK+RKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALIDAVKAVG
Sbjct: 1   MPDVQALVNDFVIKLKKRKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALIDAVKAVG 60

Query: 61  EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDDIEKDDRPVLS 120
           EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGL+L+AVSDDEDD EKDDRPVLS
Sbjct: 61  EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLNLAAVSDDEDDNEKDDRPVLS 120

Query: 121 AAAVAAAARSTLRPPSLQTLLEDVPNQTSVHQTSSSGGDSEGKSKSADKSSRSRKLKHDV 180
           AAAVAAAARSTLRPPSLQTLLEDVPNQ SVHQTSSSGGDSEGKSKSADKSSRSRKLKHDV
Sbjct: 121 AAAVAAAARSTLRPPSLQTLLEDVPNQASVHQTSSSGGDSEGKSKSADKSSRSRKLKHDV 180

Query: 181 IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA 240
           IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA
Sbjct: 181 IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA 240

Query: 241 EGAPRYQGHLLAKELVTRGLQTTLITDSAIFAMISRVNMVIVGAHAVMANGGVMAPVGLN 300
           EGAPRYQGHLLAKELVTRGLQTTLITDSA+FAMISRVNMVIVGAHAVMANGGV+APVGLN
Sbjct: 241 EGAPRYQGHLLAKELVTRGLQTTLITDSAVFAMISRVNMVIVGAHAVMANGGVIAPVGLN 300

Query: 301 MVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTNSG 360
           M+ALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTN+G
Sbjct: 301 MLALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTNTG 360

Query: 361 SPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVIKRRPSTGS 418
           SPLLHVVNPTFDYVPP+LVSLFITDTGGHNSSYMYRLI+DYYSADDLVIKRR STGS
Sbjct: 361 SPLLHVVNPTFDYVPPALVSLFITDTGGHNSSYMYRLISDYYSADDLVIKRRLSTGS 417

BLAST of HG10009694 vs. ExPASy TrEMBL
Match: A0A1S3CPD3 (translation initiation factor eIF-2B subunit beta OS=Cucumis melo OX=3656 GN=LOC103503289 PE=3 SV=1)

HSP 1 Score: 766.5 bits (1978), Expect = 5.6e-218
Identity = 409/417 (98.08%), Postives = 411/417 (98.56%), Query Frame = 0

Query: 1   MPDVQALVNDFVIKLKRRKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALIDAVKAVG 60
           MPDVQALVNDFVIKLK+RKIEGSLATAKHTAELLRSVIS QRIPYTNQAAALIDAVKAVG
Sbjct: 1   MPDVQALVNDFVIKLKKRKIEGSLATAKHTAELLRSVISAQRIPYTNQAAALIDAVKAVG 60

Query: 61  EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDDIEKDDRPVLS 120
           EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDDIEKDDRPVLS
Sbjct: 61  EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDDIEKDDRPVLS 120

Query: 121 AAAVAAAARSTLRPPSLQTLLEDVPNQTSVHQTSSSGGDSEGKSKSADKSSRSRKLKHDV 180
           AAAVAAAARSTLRPPSLQTLLEDVPNQTSVHQTSSSGGDSEGKSKSADKSSRSRKLKHDV
Sbjct: 121 AAAVAAAARSTLRPPSLQTLLEDVPNQTSVHQTSSSGGDSEGKSKSADKSSRSRKLKHDV 180

Query: 181 IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA 240
           IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA
Sbjct: 181 IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA 240

Query: 241 EGAPRYQGHLLAKELVTRGLQTTLITDSAIFAMISRVNMVIVGAHAVMANGGVMAPVGLN 300
           EGAPRYQGHLLAKEL TRGLQTTLITDSA+FAMISRVNMVIVGAHAVMANGGVMAPVGLN
Sbjct: 241 EGAPRYQGHLLAKELATRGLQTTLITDSAVFAMISRVNMVIVGAHAVMANGGVMAPVGLN 300

Query: 301 MVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTNSG 360
           MVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGT  G
Sbjct: 301 MVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTCPG 360

Query: 361 SPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVIKRRPSTGS 418
           SPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVIK R STGS
Sbjct: 361 SPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVIK-RSSTGS 416

BLAST of HG10009694 vs. ExPASy TrEMBL
Match: A0A6J1JQN6 (translation initiation factor eIF-2B subunit beta-like OS=Cucurbita maxima OX=3661 GN=LOC111487472 PE=3 SV=1)

HSP 1 Score: 766.1 bits (1977), Expect = 7.3e-218
Identity = 404/417 (96.88%), Postives = 413/417 (99.04%), Query Frame = 0

Query: 1   MPDVQALVNDFVIKLKRRKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALIDAVKAVG 60
           MPDVQALVNDFVIKLK+RKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALIDAVKAVG
Sbjct: 1   MPDVQALVNDFVIKLKKRKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALIDAVKAVG 60

Query: 61  EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDDIEKDDRPVLS 120
           EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGL+L+AVSDDEDD EKDDRPVLS
Sbjct: 61  EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLNLAAVSDDEDDNEKDDRPVLS 120

Query: 121 AAAVAAAARSTLRPPSLQTLLEDVPNQTSVHQTSSSGGDSEGKSKSADKSSRSRKLKHDV 180
           AAAVAAAARSTLRPPSLQTLLEDVPNQ SVHQTSSSGGDSEGKSKSADKSSRSRKLKHDV
Sbjct: 121 AAAVAAAARSTLRPPSLQTLLEDVPNQASVHQTSSSGGDSEGKSKSADKSSRSRKLKHDV 180

Query: 181 IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA 240
           IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA
Sbjct: 181 IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA 240

Query: 241 EGAPRYQGHLLAKELVTRGLQTTLITDSAIFAMISRVNMVIVGAHAVMANGGVMAPVGLN 300
           EGAPRYQGHLLAKELVTRGLQTTLITDSA+FAMISRVNMVIVGAHAVMANGGV+APVGLN
Sbjct: 241 EGAPRYQGHLLAKELVTRGLQTTLITDSAVFAMISRVNMVIVGAHAVMANGGVIAPVGLN 300

Query: 301 MVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTNSG 360
           M+ALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLD GEFSDCMDFGTN+G
Sbjct: 301 MLALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDIGEFSDCMDFGTNTG 360

Query: 361 SPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVIKRRPSTGS 418
           SPLLHVVNPTFDYVPP+LVSLFITDTGGHNSSYMYRLI+DYYSADDLVIKRR STGS
Sbjct: 361 SPLLHVVNPTFDYVPPALVSLFITDTGGHNSSYMYRLISDYYSADDLVIKRRLSTGS 417

BLAST of HG10009694 vs. TAIR 10
Match: AT3G07300.3 (NagB/RpiA/CoA transferase-like superfamily protein )

HSP 1 Score: 638.3 bits (1645), Expect = 4.4e-183
Identity = 340/411 (82.73%), Postives = 369/411 (89.78%), Query Frame = 0

Query: 1   MPDVQALVNDFVIKLKRRKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALIDAVKAVG 60
           MPDVQ+ V +FV KL++RKIEGS ATAK+T ELLRSVIS QR+P+ NQAAALIDAVKAVG
Sbjct: 1   MPDVQSTVVEFVNKLRKRKIEGSQATAKYTVELLRSVISHQRVPHANQAAALIDAVKAVG 60

Query: 61  EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDE--DDIEKDDRPV 120
           EQLIAANPVELAVGN+VRRVLHIIREEDLSLTTA++ GL L   SDD+  D+ +    P 
Sbjct: 61  EQLIAANPVELAVGNVVRRVLHIIREEDLSLTTAAMGGLDLLDASDDDDVDNCKGIGFPA 120

Query: 121 LSAAAVAAAARSTLRPPSLQTLLEDVPNQTSVHQTSSSGGDSEGKSKSADKSSRSRKLKH 180
           +SAA VAAAARSTLRPPSLQTLLE  P   +V  TSSSG DSE KSKSADKSS +RKLKH
Sbjct: 121 MSAAVVAAAARSTLRPPSLQTLLEGTPESATVPYTSSSGADSESKSKSADKSSLTRKLKH 180

Query: 181 DVIEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVF 240
           DVIE VN+LIQ+IA CHEQIAEQA+EHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVF
Sbjct: 181 DVIEGVNQLIQEIAGCHEQIAEQAIEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVF 240

Query: 241 VAEGAPRYQGHLLAKELVTRGLQTTLITDSAIFAMISRVNMVIVGAHAVMANGGVMAPVG 300
           VAEGAPRYQGHLLAKELV RGLQTT+ITDSA+FAMISRVNMVI+GAHAVMANGGV+ PVG
Sbjct: 241 VAEGAPRYQGHLLAKELVARGLQTTVITDSAVFAMISRVNMVIIGAHAVMANGGVIGPVG 300

Query: 301 LNMVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTN 360
           +NM ALAA++HAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDC+DFG  
Sbjct: 301 VNMAALAAQKHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCLDFGAG 360

Query: 361 SGSPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVI 410
           SGS  L VVNPTFDYVPP+LVSLFITDTGGHN SYMYRLIADYYSADDLV+
Sbjct: 361 SGS--LQVVNPTFDYVPPNLVSLFITDTGGHNPSYMYRLIADYYSADDLVM 409

BLAST of HG10009694 vs. TAIR 10
Match: AT3G07300.1 (NagB/RpiA/CoA transferase-like superfamily protein )

HSP 1 Score: 630.6 bits (1625), Expect = 9.2e-181
Identity = 338/411 (82.24%), Postives = 368/411 (89.54%), Query Frame = 0

Query: 1   MPDVQALVNDFVIKLKRRKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALIDAVKAVG 60
           MPDVQ+ V +FV KL++RKIEGS ATAK+T ELLRSVIS QR+P+ NQAAALIDAVKAVG
Sbjct: 1   MPDVQSTVVEFVNKLRKRKIEGSQATAKYTVELLRSVISHQRVPHANQAAALIDAVKAVG 60

Query: 61  EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDE--DDIEKDDRPV 120
           EQLIAANPVELAVGN+VRRVLHIIREEDLSLTTA++ GL L   SDD+  D+ +    P 
Sbjct: 61  EQLIAANPVELAVGNVVRRVLHIIREEDLSLTTAAMGGLDLLDASDDDDVDNCKGIGFPA 120

Query: 121 LSAAAVAAAARSTLRPPSLQTLLEDVPNQTSVHQTSSSGGDSEGKSKSADKSSRSRKLKH 180
           +SAA VAAAARSTLRPPSLQTLLE  P   +V  TSSSG DSE  SK+ADKSS +RKLKH
Sbjct: 121 MSAAVVAAAARSTLRPPSLQTLLEGTPESATVPYTSSSGADSE--SKTADKSSLTRKLKH 180

Query: 181 DVIEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVF 240
           DVIE VN+LIQ+IA CHEQIAEQA+EHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVF
Sbjct: 181 DVIEGVNQLIQEIAGCHEQIAEQAIEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVF 240

Query: 241 VAEGAPRYQGHLLAKELVTRGLQTTLITDSAIFAMISRVNMVIVGAHAVMANGGVMAPVG 300
           VAEGAPRYQGHLLAKELV RGLQTT+ITDSA+FAMISRVNMVI+GAHAVMANGGV+ PVG
Sbjct: 241 VAEGAPRYQGHLLAKELVARGLQTTVITDSAVFAMISRVNMVIIGAHAVMANGGVIGPVG 300

Query: 301 LNMVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTN 360
           +NM ALAA++HAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDC+DFG  
Sbjct: 301 VNMAALAAQKHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCLDFGAG 360

Query: 361 SGSPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVI 410
           SGS  L VVNPTFDYVPP+LVSLFITDTGGHN SYMYRLIADYYSADDLV+
Sbjct: 361 SGS--LQVVNPTFDYVPPNLVSLFITDTGGHNPSYMYRLIADYYSADDLVM 407

BLAST of HG10009694 vs. TAIR 10
Match: AT3G07300.2 (NagB/RpiA/CoA transferase-like superfamily protein )

HSP 1 Score: 630.6 bits (1625), Expect = 9.2e-181
Identity = 338/411 (82.24%), Postives = 368/411 (89.54%), Query Frame = 0

Query: 1   MPDVQALVNDFVIKLKRRKIEGSLATAKHTAELLRSVISQQRIPYTNQAAALIDAVKAVG 60
           MPDVQ+ V +FV KL++RKIEGS ATAK+T ELLRSVIS QR+P+ NQAAALIDAVKAVG
Sbjct: 1   MPDVQSTVVEFVNKLRKRKIEGSQATAKYTVELLRSVISHQRVPHANQAAALIDAVKAVG 60

Query: 61  EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDE--DDIEKDDRPV 120
           EQLIAANPVELAVGN+VRRVLHIIREEDLSLTTA++ GL L   SDD+  D+ +    P 
Sbjct: 61  EQLIAANPVELAVGNVVRRVLHIIREEDLSLTTAAMGGLDLLDASDDDDVDNCKGIGFPA 120

Query: 121 LSAAAVAAAARSTLRPPSLQTLLEDVPNQTSVHQTSSSGGDSEGKSKSADKSSRSRKLKH 180
           +SAA VAAAARSTLRPPSLQTLLE  P   +V  TSSSG DSE  SK+ADKSS +RKLKH
Sbjct: 121 MSAAVVAAAARSTLRPPSLQTLLEGTPESATVPYTSSSGADSE--SKTADKSSLTRKLKH 180

Query: 181 DVIEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVF 240
           DVIE VN+LIQ+IA CHEQIAEQA+EHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVF
Sbjct: 181 DVIEGVNQLIQEIAGCHEQIAEQAIEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVF 240

Query: 241 VAEGAPRYQGHLLAKELVTRGLQTTLITDSAIFAMISRVNMVIVGAHAVMANGGVMAPVG 300
           VAEGAPRYQGHLLAKELV RGLQTT+ITDSA+FAMISRVNMVI+GAHAVMANGGV+ PVG
Sbjct: 241 VAEGAPRYQGHLLAKELVARGLQTTVITDSAVFAMISRVNMVIIGAHAVMANGGVIGPVG 300

Query: 301 LNMVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTN 360
           +NM ALAA++HAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDC+DFG  
Sbjct: 301 VNMAALAAQKHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCLDFGAG 360

Query: 361 SGSPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVI 410
           SGS  L VVNPTFDYVPP+LVSLFITDTGGHN SYMYRLIADYYSADDLV+
Sbjct: 361 SGS--LQVVNPTFDYVPPNLVSLFITDTGGHNPSYMYRLIADYYSADDLVM 407

BLAST of HG10009694 vs. TAIR 10
Match: AT2G44070.1 (NagB/RpiA/CoA transferase-like superfamily protein )

HSP 1 Score: 92.8 bits (229), Expect = 6.9e-19
Identity = 68/219 (31.05%), Postives = 102/219 (46.58%), Query Frame = 0

Query: 169 KSSRSRKLKHDVIEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAA 228
           +S     L  D+   +NE I    T    I E AV  I   +V+LT GSS  +   L  A
Sbjct: 91  ESEAKASLHSDIERFINEKIIGADTV---IVEHAVTKIRDGDVLLTYGSSTVIEMILVHA 150

Query: 229 KEKKRSFRVFVAEGAPRYQGHLLAKELVTRGLQTTLITDSAIFAMISRVNMVIVGAHAVM 288
            E  + FRVFV +  P+ QG LL + L+ RG+  T    +AI  ++ +V  V +GA +V 
Sbjct: 151 HELGKKFRVFVVDSRPKLQGKLLLRRLIKRGINCTYTHITAISYIMHQVTKVFLGASSVF 210

Query: 289 ANGGVMAPVGLNMVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGE 348
           +NG V + VG   VA+ A    VP +V   ++K       +  +  NEL  P+ +     
Sbjct: 211 SNGTVYSRVGTACVAMVANAFRVPVLVCCEAYKFHEKVQLDYSICSNELGDPNAISKVHG 270

Query: 349 FSDCMDFGTNSGSPLLHVVNPTFDYVPPSLVSLFITDTG 388
             D       + +  L  +N  +D  P   +S+ ITD G
Sbjct: 271 REDINYLNGLTNNAKLKFLNLMYDATPSDYISMIITDYG 306

BLAST of HG10009694 vs. TAIR 10
Match: AT5G38640.1 (NagB/RpiA/CoA transferase-like superfamily protein )

HSP 1 Score: 84.7 bits (208), Expect = 1.9e-16
Identity = 69/226 (30.53%), Postives = 106/226 (46.90%), Query Frame = 0

Query: 169 KSSRSRKLKHDVIEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAA 228
           +S     L+ D+   +NE    I      I + AV  I   +V+LT GS   V   +  A
Sbjct: 407 ESEAKATLQSDIERFINE---KIIVADTVIVKHAVTKIRDGDVLLTYGSPTAVEMVILHA 466

Query: 229 KEKKRSFRVFVAEGAPRYQGHLLAKELVTRGLQTTLITDSAIFAMISRVNMVIVGAHAVM 288
            E ++ FRV V +  P+ +G LL + L+ RG+  T    +AI  ++  V  V +GA +V+
Sbjct: 467 HELRKKFRVLVVDSRPKLEGQLLLRRLIKRGINCTYTHINAISYIMHEVTKVFLGASSVL 526

Query: 289 ANGGVMAPVGLNMVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLL-----NELRSPSEL 348
           +NG V + VG   VA+ A    VP +V       C  Y  +  V L     NEL  P+ +
Sbjct: 527 SNGTVYSRVGTACVAMVANAFRVPVLV------CCEAYKFHERVQLDSICSNELGDPNAI 586

Query: 349 LDFGEFSDC--MDFGTNSGSPLLHVVNPTFDYVPPSLVSLFITDTG 388
            +     D   +D  TN+ +  L  +N  +D  P   +S+ ITD G
Sbjct: 587 SEVHGREDINYLDGLTNNAN--LQFLNLMYDATPSDYISMIITDYG 621

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038874367.11.0e-22198.80translation initiation factor eIF-2B subunit beta [Benincasa hispida] >XP_038874... [more]
XP_022141443.19.4e-22097.60translation initiation factor eIF-2B subunit beta-like [Momordica charantia][more]
XP_022939685.11.4e-21897.36translation initiation factor eIF-2B subunit beta-like [Cucurbita moschata][more]
XP_023551232.12.3e-21897.36translation initiation factor eIF-2B subunit beta-like [Cucurbita pepo subsp. pe... [more]
XP_022923286.13.0e-21897.12translation initiation factor eIF-2B subunit beta-like [Cucurbita moschata] >XP_... [more]
Match NameE-valueIdentityDescription
Q54EY27.1e-6941.90Translation initiation factor eIF-2B subunit beta OS=Dictyostelium discoideum OX... [more]
Q9UT761.0e-5936.83Probable translation initiation factor eIF-2B subunit beta OS=Schizosaccharomyce... [more]
Q905116.9e-5638.83Translation initiation factor eIF-2B subunit beta OS=Takifugu rubripes OX=31033 ... [more]
Q5E9B41.9e-5338.71Translation initiation factor eIF-2B subunit beta OS=Bos taurus OX=9913 GN=EIF2B... [more]
Q99LD93.2e-5338.96Translation initiation factor eIF-2B subunit beta OS=Mus musculus OX=10090 GN=Ei... [more]
Match NameE-valueIdentityDescription
A0A6J1CKI14.6e-22097.60translation initiation factor eIF-2B subunit beta-like OS=Momordica charantia OX... [more]
A0A6J1FNF36.6e-21997.36translation initiation factor eIF-2B subunit beta-like OS=Cucurbita moschata OX=... [more]
A0A6J1E6E71.5e-21897.12translation initiation factor eIF-2B subunit beta-like OS=Cucurbita moschata OX=... [more]
A0A1S3CPD35.6e-21898.08translation initiation factor eIF-2B subunit beta OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A6J1JQN67.3e-21896.88translation initiation factor eIF-2B subunit beta-like OS=Cucurbita maxima OX=36... [more]
Match NameE-valueIdentityDescription
AT3G07300.34.4e-18382.73NagB/RpiA/CoA transferase-like superfamily protein [more]
AT3G07300.19.2e-18182.24NagB/RpiA/CoA transferase-like superfamily protein [more]
AT3G07300.29.2e-18182.24NagB/RpiA/CoA transferase-like superfamily protein [more]
AT2G44070.16.9e-1931.05NagB/RpiA/CoA transferase-like superfamily protein [more]
AT5G38640.11.9e-1630.53NagB/RpiA/CoA transferase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000649Initiation factor 2B-relatedPFAMPF01008IF-2Bcoord: 165..389
e-value: 6.0E-63
score: 212.7
coord: 19..122
e-value: 3.8E-6
score: 26.3
IPR042529Initiation factor 2B-like, C-terminalGENE3D3.40.50.10470coord: 194..401
e-value: 2.4E-72
score: 244.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 142..176
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 142..161
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 162..176
NoneNo IPR availablePANTHERPTHR45859TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT BETAcoord: 54..409
NoneNo IPR availablePANTHERPTHR45859:SF4BNAA01G32370D PROTEINcoord: 54..409
IPR037171NagB/RpiA transferase-likeSUPERFAMILY100950NagB/RpiA/CoA transferase-likecoord: 7..406

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10009694.1HG10009694.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050790 regulation of catalytic activity
biological_process GO:0006413 translational initiation
biological_process GO:0044237 cellular metabolic process
cellular_component GO:0005851 eukaryotic translation initiation factor 2B complex
molecular_function GO:0005085 guanyl-nucleotide exchange factor activity
molecular_function GO:0003743 translation initiation factor activity