HG10009638 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10009638 vs. NCBI nr
Match: XP_038875429.1 (protoporphyrinogen oxidase 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 912.5 bits (2357), Expect = 1.5e-261
Identity = 467/549 (85.06%), Postives = 474/549 (86.34%), Query Frame = 0

Query: 1   MATGATLLTDLPFRRPHPLTLLRPSNIPSYHPLHISLRNNHLRPNFRCSIAEGSTALSPS 60
           MATGATLLTDLPFRRPHPLTLLRPSNIPS+HPLH+SLRNNHLRPNFRCSIAEGSTALSPS
Sbjct: 1   MATGATLLTDLPFRRPHPLTLLRPSNIPSFHPLHVSLRNNHLRPNFRCSIAEGSTALSPS 60

Query: 61  KASGQSSM---------------------------------------------------- 120
           KASGQSSM                                                    
Sbjct: 61  KASGQSSMVDCVVVGGGISGLCIAQALQTKHPNVAPNIIVTEAKDRVGGNITTVERDGYL 120

Query: 121 ------------------VDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLM 180
                             VDSGLKDDLVLGDPDAPRFVLWNGKLRPVP KP DLPFFDLM
Sbjct: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLM 180

Query: 181 SLGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSM 240
           SLGGKIRAGFGALGIRPPPPGREESVEEFVRRNLG+EVFERLIEPFCSGVYAGDPSKLSM
Sbjct: 181 SLGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM 240

Query: 241 KAAFGKVWRLEQNGGSIIGGTFKAIQERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPN 300
           KAAFGKVW+LEQNGGSIIGGTFKAIQERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPN
Sbjct: 241 KAAFGKVWKLEQNGGSIIGGTFKAIQERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPN 300

Query: 301 AISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLVSILSRSIIMTVPSYIAGTLLRP 360
           AISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLVSILSRS+IMTVPSYIAGTLLRP
Sbjct: 301 AISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYIAGTLLRP 360

Query: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420
           ISGKAADALSKFYYPPVASVTISYPKGAIRKECL DGELKGFGQLHPRSQGVTTLGTIYS
Sbjct: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLSDGELKGFGQLHPRSQGVTTLGTIYS 420

Query: 421 SSLFPNRAPDGRVLLLNYIGGATNTEILSKTESELVEVVDRDLRKILINPNTEDPLSLSV 480
           SSLFPNRAPDGRVLLLNYIGGATNTEILSKTESELVEVVD+DLRKILINPN EDPLSLSV
Sbjct: 421 SSLFPNRAPDGRVLLLNYIGGATNTEILSKTESELVEVVDQDLRKILINPNAEDPLSLSV 480

BLAST of HG10009638 vs. NCBI nr
Match: XP_004149479.1 (protoporphyrinogen oxidase 1, chloroplastic [Cucumis sativus] >KGN49787.1 hypothetical protein Csa_004680 [Cucumis sativus])

HSP 1 Score: 890.2 bits (2299), Expect = 8.0e-255
Identity = 455/549 (82.88%), Postives = 467/549 (85.06%), Query Frame = 0

Query: 1   MATGATLLTDLPFRRPHPLTLLRPSNIPSYHPLHISLRNNHLRPNFRCSIAEGSTALSPS 60
           MATG+TLLTDLPFRRPHPLTLLRPSNIPS+HPLHISL+NN LR  FRCSIAEGSTALSPS
Sbjct: 1   MATGSTLLTDLPFRRPHPLTLLRPSNIPSFHPLHISLQNNRLRSYFRCSIAEGSTALSPS 60

Query: 61  KASGQSS----------------------------------------------------- 120
            AS QSS                                                     
Sbjct: 61  NASSQSSILDCVVVGAGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYL 120

Query: 121 -----------------MVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLM 180
                            +VDSGLKDDLVLGDPDAPRFVLWNGKLRPVP KP DLPFFDLM
Sbjct: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLM 180

Query: 181 SLGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSM 240
           S+GGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSM
Sbjct: 181 SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSM 240

Query: 241 KAAFGKVWRLEQNGGSIIGGTFKAIQERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPN 300
           KAAFGKVWRLEQNGGSIIGGTFKA+QERNK TKPPRDPRLPKPKGQTVGSFRKGL MLPN
Sbjct: 241 KAAFGKVWRLEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPN 300

Query: 301 AISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLVSILSRSIIMTVPSYIAGTLLRP 360
           AISTCLGSKVK+SWKLSSISKVDDGGYSLTYETPEGLVSILSRS+IMTVPSYIAGTLLRP
Sbjct: 301 AISTCLGSKVKVSWKLSSISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYIAGTLLRP 360

Query: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420
           ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS
Sbjct: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420

Query: 421 SSLFPNRAPDGRVLLLNYIGGATNTEILSKTESELVEVVDRDLRKILINPNTEDPLSLSV 480
           SSLFPNRAPDGRVLLLNYIGGATNT ILS+TESEL+EVVDRDLRKILINPN EDPL LSV
Sbjct: 421 SSLFPNRAPDGRVLLLNYIGGATNTGILSQTESELIEVVDRDLRKILINPNAEDPLPLSV 480

BLAST of HG10009638 vs. NCBI nr
Match: KAA0050262.1 (protoporphyrinogen oxidase 1 [Cucumis melo var. makuwa] >TYJ98146.1 protoporphyrinogen oxidase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 887.5 bits (2292), Expect = 5.2e-254
Identity = 454/549 (82.70%), Postives = 467/549 (85.06%), Query Frame = 0

Query: 1   MATGATLLTDLPFRRPHPLTLLRPSNIPSYHPLHISLRNNHLRPNFRCSIAEGSTALSPS 60
           MATGATLLTDLPFRRPHPLTLLRPS+IPS++PLHISL+NN LRP+FRCSIAEGSTALSPS
Sbjct: 1   MATGATLLTDLPFRRPHPLTLLRPSDIPSFYPLHISLQNNRLRPHFRCSIAEGSTALSPS 60

Query: 61  KASGQSS----------------------------------------------------- 120
            AS QSS                                                     
Sbjct: 61  NASSQSSILDCVVVGGGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYL 120

Query: 121 -----------------MVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLM 180
                            +VDSGLKDDLVLGDPDAPRFVLWNGKLRPVP KP DLPFFDLM
Sbjct: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLM 180

Query: 181 SLGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSM 240
           S+GGKIRAGFGALGIRPPPPGREESVEEFVRRNLG+EVFERLIEPFCSGVYAGDPSKLSM
Sbjct: 181 SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM 240

Query: 241 KAAFGKVWRLEQNGGSIIGGTFKAIQERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPN 300
           KAAFGKVWRLEQNGGSIIGGTFKA+QERNK TKPPRDPRLPKPKGQTVGSFRKGL MLPN
Sbjct: 241 KAAFGKVWRLEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPN 300

Query: 301 AISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLVSILSRSIIMTVPSYIAGTLLRP 360
           AISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLV ILSRS+IMTVPSYIAGTLLRP
Sbjct: 301 AISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLVPILSRSVIMTVPSYIAGTLLRP 360

Query: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420
           ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS
Sbjct: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420

Query: 421 SSLFPNRAPDGRVLLLNYIGGATNTEILSKTESELVEVVDRDLRKILINPNTEDPLSLSV 480
           SSLFPNRAPDGRVLLLNYIGGATNT ILS+ ESEL+EVVDRDLRKILINPN EDPL LSV
Sbjct: 421 SSLFPNRAPDGRVLLLNYIGGATNTGILSQKESELIEVVDRDLRKILINPNAEDPLPLSV 480

BLAST of HG10009638 vs. NCBI nr
Match: XP_008466230.1 (PREDICTED: protoporphyrinogen oxidase 1, chloroplastic [Cucumis melo])

HSP 1 Score: 884.4 bits (2284), Expect = 4.4e-253
Identity = 453/549 (82.51%), Postives = 466/549 (84.88%), Query Frame = 0

Query: 1   MATGATLLTDLPFRRPHPLTLLRPSNIPSYHPLHISLRNNHLRPNFRCSIAEGSTALSPS 60
           MATGATLLTDLPFRRPHPLTLLRPS+IPS++PLHISL+NN LR +FRCSIAEGSTALSPS
Sbjct: 1   MATGATLLTDLPFRRPHPLTLLRPSDIPSFYPLHISLQNNRLRSHFRCSIAEGSTALSPS 60

Query: 61  KASGQSS----------------------------------------------------- 120
            AS QSS                                                     
Sbjct: 61  NASSQSSILDCVVVGGGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYL 120

Query: 121 -----------------MVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLM 180
                            +VDSGLKDDLVLGDPDAPRFVLWNGKLRPVP KP DLPFFDLM
Sbjct: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLM 180

Query: 181 SLGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSM 240
           S+GGKIRAGFGALGIRPPPPGREESVEEFVRRNLG+EVFERLIEPFCSGVYAGDPSKLSM
Sbjct: 181 SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM 240

Query: 241 KAAFGKVWRLEQNGGSIIGGTFKAIQERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPN 300
           KAAFGKVWRLEQNGGSIIGGTFKA+QERNK TKPPRDPRLPKPKGQTVGSFRKGL MLPN
Sbjct: 241 KAAFGKVWRLEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPN 300

Query: 301 AISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLVSILSRSIIMTVPSYIAGTLLRP 360
           AISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLV ILSRS+IMTVPSYIAGTLLRP
Sbjct: 301 AISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLVPILSRSVIMTVPSYIAGTLLRP 360

Query: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420
           ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS
Sbjct: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420

Query: 421 SSLFPNRAPDGRVLLLNYIGGATNTEILSKTESELVEVVDRDLRKILINPNTEDPLSLSV 480
           SSLFPNRAPDGRVLLLNYIGGATNT ILS+ ESEL+EVVDRDLRKILINPN EDPL LSV
Sbjct: 421 SSLFPNRAPDGRVLLLNYIGGATNTGILSQKESELIEVVDRDLRKILINPNAEDPLPLSV 480

BLAST of HG10009638 vs. NCBI nr
Match: KAG7016666.1 (Protoporphyrinogen oxidase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 860.5 bits (2222), Expect = 6.7e-246
Identity = 442/549 (80.51%), Postives = 457/549 (83.24%), Query Frame = 0

Query: 1   MATGATLLTDLPFRRPHPLTLLRPSNIPSYHPLHISLRNNHLRPNFRCSIAEGSTALSPS 60
           MA+GATLLTDLPFRRP+P TL RPSNIPS+HPLHIS+R NHLRPNFRCSIAEGST +S S
Sbjct: 1   MASGATLLTDLPFRRPYPPTLFRPSNIPSFHPLHISIRINHLRPNFRCSIAEGSTTVSTS 60

Query: 61  KASGQSS----------------------------------------------------- 120
             SGQSS                                                     
Sbjct: 61  DVSGQSSRVDCVVVGGGISGLCIAQALATKHPDVAPNIIVTEAKARVGGNITTVERDGYL 120

Query: 121 -----------------MVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLM 180
                            +VDSGLKDDLVLGDPDAPRFVLW GKLRPVP KP DLPFFDLM
Sbjct: 121 WEEGPNSFQPSDPLLTMVVDSGLKDDLVLGDPDAPRFVLWKGKLRPVPAKPNDLPFFDLM 180

Query: 181 SLGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSM 240
           SLGGKIRAGFGALGIRPPPPGREESVEEFVRRNLG+EVFERLIEPFCSGVYAGDPSKLSM
Sbjct: 181 SLGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM 240

Query: 241 KAAFGKVWRLEQNGGSIIGGTFKAIQERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPN 300
           KAAFGKVW+LEQNGGSIIGGTFKA QERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPN
Sbjct: 241 KAAFGKVWKLEQNGGSIIGGTFKAFQERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPN 300

Query: 301 AISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLVSILSRSIIMTVPSYIAGTLLRP 360
           AISTCLG+KVKLSWKLS+ISKVDDG YSLTYETPEGLVSILSRS+IMTVPSY+A TLLRP
Sbjct: 301 AISTCLGNKVKLSWKLSNISKVDDGSYSLTYETPEGLVSILSRSVIMTVPSYVASTLLRP 360

Query: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420
           IS KAADALSKFYYPPVASVTISYPKGAIRKECLI GELKGFGQLHPRSQGVTTLGTIYS
Sbjct: 361 ISVKAADALSKFYYPPVASVTISYPKGAIRKECLIGGELKGFGQLHPRSQGVTTLGTIYS 420

Query: 421 SSLFPNRAPDGRVLLLNYIGGATNTEILSKTESELVEVVDRDLRKILINPNTEDPLSLSV 480
           SSLFPNRAPDGRVLLLNYIGGATNTEILSKTESELVEVVDRDLRKILIN N EDP+ LSV
Sbjct: 421 SSLFPNRAPDGRVLLLNYIGGATNTEILSKTESELVEVVDRDLRKILINTNAEDPMLLSV 480

BLAST of HG10009638 vs. ExPASy Swiss-Prot
Match: P55826 (Protoporphyrinogen oxidase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PPOX1 PE=1 SV=1)

HSP 1 Score: 691.4 bits (1783), Expect = 7.1e-198
Identity = 340/428 (79.44%), Postives = 374/428 (87.38%), Query Frame = 0

Query: 52  EGSTALSPSKASGQSSMVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLMS 111
           EG  +  PS     + +VDSGLKDDLVLGDP APRFVLWNGKLRPVP K TDLPFFDLMS
Sbjct: 111 EGPNSFQPSDPM-LTMVVDSGLKDDLVLGDPTAPRFVLWNGKLRPVPSKLTDLPFFDLMS 170

Query: 112 LGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSMK 171
           +GGKIRAGFGALGIRP PPGREESVEEFVRRNLG+EVFERLIEPFCSGVYAGDPSKLSMK
Sbjct: 171 IGGKIRAGFGALGIRPSPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMK 230

Query: 172 AAFGKVWRLEQNGGSIIGGTFKAIQERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPNA 231
           AAFGKVW+LEQNGGSIIGGTFKAIQER  A K  RDPRLPKP+GQTVGSFRKGL MLP A
Sbjct: 231 AAFGKVWKLEQNGGSIIGGTFKAIQERKNAPKAERDPRLPKPQGQTVGSFRKGLRMLPEA 290

Query: 232 ISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLVSILSRSIIMTVPSYIAGTLLRPI 291
           IS  LGSKVKLSWKLS I+K++ GGY+LTYETP+GLVS+ S+S++MTVPS++A  LLRP+
Sbjct: 291 ISARLGSKVKLSWKLSGITKLESGGYNLTYETPDGLVSVQSKSVVMTVPSHVASGLLRPL 350

Query: 292 SGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSS 351
           S  AA+ALSK YYPPVA+V+ISYPK AIR ECLIDGELKGFGQLHPR+QGV TLGTIYSS
Sbjct: 351 SESAANALSKLYYPPVAAVSISYPKEAIRTECLIDGELKGFGQLHPRTQGVETLGTIYSS 410

Query: 352 SLFPNRAPDGRVLLLNYIGGATNTEILSKTESELVEVVDRDLRKILINPNTEDPLSLSVR 411
           SLFPNRAP GR+LLLNYIGG+TNT ILSK+E ELVE VDRDLRK+LI PN+ DPL L VR
Sbjct: 411 SLFPNRAPPGRILLLNYIGGSTNTGILSKSEGELVEAVDRDLRKMLIKPNSTDPLKLGVR 470

Query: 412 VWPQAIPQFLIGHLDLLDTAKAGLRDAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVAG 471
           VWPQAIPQFL+GH D+LDTAK+ L  +G EGLFLGGNYV GVALGRCVEGAYE A EV  
Sbjct: 471 VWPQAIPQFLVGHFDILDTAKSSLTSSGYEGLFLGGNYVAGVALGRCVEGAYETAIEVNN 530

Query: 472 FLSKKVYK 480
           F+S+  YK
Sbjct: 531 FMSRYAYK 537

BLAST of HG10009638 vs. ExPASy Swiss-Prot
Match: O24163 (Protoporphyrinogen oxidase, chloroplastic OS=Nicotiana tabacum OX=4097 GN=PPXI PE=2 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 1.2e-192
Identity = 332/428 (77.57%), Postives = 368/428 (85.98%), Query Frame = 0

Query: 52  EGSTALSPSKASGQSSMVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLMS 111
           EG  +  PS     +  VD GLKDDLVLGDP+APRFVLW GKLRPVP K TDL FFDLMS
Sbjct: 122 EGPNSFQPSDPM-LTMAVDCGLKDDLVLGDPNAPRFVLWKGKLRPVPSKLTDLAFFDLMS 181

Query: 112 LGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSMK 171
           + GK+RAGFGA+G+RP PPG EESVE+FVRRNLG EVFERLIEPFCSGVYAGDPSKLSMK
Sbjct: 182 IPGKLRAGFGAIGLRPSPPGHEESVEQFVRRNLGGEVFERLIEPFCSGVYAGDPSKLSMK 241

Query: 172 AAFGKVWRLEQNGGSIIGGTFKAIQERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPNA 231
           AAFGKVW+LE+ GGSIIGGTFKAI+ER+   K PRDPRLPKPKGQTVGSFRKGL MLP+A
Sbjct: 242 AAFGKVWKLEETGGSIIGGTFKAIKERSSTPKAPRDPRLPKPKGQTVGSFRKGLRMLPDA 301

Query: 232 ISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLVSILSRSIIMTVPSYIAGTLLRPI 291
           IS  LGSK+KLSWKLSSI+K + GGY LTYETPEG+VS+ SRSI+MTVPSY+A  +LRP+
Sbjct: 302 ISARLGSKLKLSWKLSSITKSEKGGYHLTYETPEGVVSLQSRSIVMTVPSYVASNILRPL 361

Query: 292 SGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSS 351
           S  AADALS FYYPPV +VTI+YP+ AIR E L+DGELKGFGQLHPR+QGV TLGTIYSS
Sbjct: 362 SVAAADALSNFYYPPVGAVTITYPQEAIRDERLVDGELKGFGQLHPRTQGVETLGTIYSS 421

Query: 352 SLFPNRAPDGRVLLLNYIGGATNTEILSKTESELVEVVDRDLRKILINPNTEDPLSLSVR 411
           SLFPNRAP GRVLLLNYIGGA N EILSKTES+LVEVVDRDLRK+LI P  +DPL + VR
Sbjct: 422 SLFPNRAPKGRVLLLNYIGGAKNPEILSKTESQLVEVVDRDLRKMLIKPKAQDPLVVGVR 481

Query: 412 VWPQAIPQFLIGHLDLLDTAKAGLRDAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVAG 471
           VWPQAIPQFL+GHLD L TAKA + D G+EGLFLGGNYV GVALGRCVEGAYE A+EV G
Sbjct: 482 VWPQAIPQFLVGHLDTLSTAKAAMNDNGLEGLFLGGNYVSGVALGRCVEGAYEVASEVTG 541

Query: 472 FLSKKVYK 480
           FLS+  YK
Sbjct: 542 FLSRYAYK 548

BLAST of HG10009638 vs. ExPASy Swiss-Prot
Match: Q9AR38 (Protoporphyrinogen oxidase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=PPOX1 PE=2 SV=1)

HSP 1 Score: 669.5 bits (1726), Expect = 2.9e-191
Identity = 327/428 (76.40%), Postives = 368/428 (85.98%), Query Frame = 0

Query: 52  EGSTALSPSKASGQSSMVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLMS 111
           EG  +  PS     +  VDSGLKDDLV GDP+APRFVLW GKLRPVP KP DLPFFDLMS
Sbjct: 110 EGPNSFQPSDPV-LTMAVDSGLKDDLVFGDPNAPRFVLWEGKLRPVPSKPGDLPFFDLMS 169

Query: 112 LGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSMK 171
           + GK+RAG GALG+R PPPGREESVE+FVRRNLG EVFERLIEPFCSGVYAGDPSKLSMK
Sbjct: 170 IPGKLRAGLGALGVRAPPPGREESVEDFVRRNLGAEVFERLIEPFCSGVYAGDPSKLSMK 229

Query: 172 AAFGKVWRLEQNGGSIIGGTFKAIQERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPNA 231
           AAFGKVWRLE  GGSIIGGT K IQER K  KPPRDPRLP PKGQTV SFRKGL MLP+A
Sbjct: 230 AAFGKVWRLEDTGGSIIGGTIKTIQERGKNPKPPRDPRLPTPKGQTVASFRKGLTMLPDA 289

Query: 232 ISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLVSILSRSIIMTVPSYIAGTLLRPI 291
           I++ LGSKVKLSWKL+SI+K D+ GY+L YETPEG+VS+ +++++MT+PSY+A  +LRP+
Sbjct: 290 ITSRLGSKVKLSWKLTSITKSDNKGYALVYETPEGVVSVQAKTVVMTIPSYVASDILRPL 349

Query: 292 SGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSS 351
           S  AADALS FYYPPVA+VT+SYPK AIRKECLIDGEL+GFGQLHPRSQGV TLGTIYSS
Sbjct: 350 SSDAADALSIFYYPPVAAVTVSYPKEAIRKECLIDGELQGFGQLHPRSQGVETLGTIYSS 409

Query: 352 SLFPNRAPDGRVLLLNYIGGATNTEILSKTESELVEVVDRDLRKILINPNTEDPLSLSVR 411
           SLFPNRAP GRVLLLNYIGG+TNT I+SKTESELVE VDRDLRK+LINP   DPL L VR
Sbjct: 410 SLFPNRAPAGRVLLLNYIGGSTNTGIVSKTESELVEAVDRDLRKMLINPKAVDPLVLGVR 469

Query: 412 VWPQAIPQFLIGHLDLLDTAKAGLRDAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVAG 471
           VWPQAIPQFLIGHLD L+ AK+ L   G +GLFLGGNYV GVALGRCVEGAYE+A++++ 
Sbjct: 470 VWPQAIPQFLIGHLDHLEAAKSALGKGGYDGLFLGGNYVAGVALGRCVEGAYESASQISD 529

Query: 472 FLSKKVYK 480
           +L+K  YK
Sbjct: 530 YLTKYAYK 536

BLAST of HG10009638 vs. ExPASy Swiss-Prot
Match: Q9LRI8 (Protoporphyrinogen oxidase 1, chloroplastic OS=Spinacia oleracea OX=3562 GN=POX1 PE=1 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 1.5e-184
Identity = 319/424 (75.24%), Postives = 358/424 (84.43%), Query Frame = 0

Query: 52  EGSTALSPSKASGQSSMVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLMS 111
           EG  +  PS A   +  VDSGLK++LVLGDP++PRFVLWNGKLRPVP K TDLPFFDLMS
Sbjct: 136 EGPNSFQPSDAV-LTMAVDSGLKEELVLGDPNSPRFVLWNGKLRPVPSKLTDLPFFDLMS 195

Query: 112 LGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSMK 171
             GKIRAG GALG+RP PP  EESVE+FVRRNLG+EVFERLIEPFCSGVYAGDPSKLSMK
Sbjct: 196 FPGKIRAGLGALGLRPSPPAHEESVEQFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMK 255

Query: 172 AAFGKVWRLEQNGGSIIGGTFKAIQERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPNA 231
           AAFG+VW LEQ GGSIIGGT K IQER    KPPRDPRLPKPKGQTVGSFRKGL+MLP A
Sbjct: 256 AAFGRVWVLEQKGGSIIGGTLKTIQERKDNPKPPRDPRLPKPKGQTVGSFRKGLSMLPTA 315

Query: 232 ISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLVSILSRSIIMTVPSYIAGTLLRPI 291
           IS  LG+KVK+SW LS I+K  +G Y+LTYETP+GLVS+ ++S++MTVPSY+A +LLRP+
Sbjct: 316 ISERLGNKVKVSWTLSGIAKSSNGEYNLTYETPDGLVSVRTKSVVMTVPSYVASSLLRPL 375

Query: 292 SGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSS 351
           S  AA++LSKF+YPPVA+V++SYPK AIR ECLIDGELKGFGQLH RSQGV TLGTIYSS
Sbjct: 376 SDVAAESLSKFHYPPVAAVSLSYPKEAIRSECLIDGELKGFGQLHSRSQGVETLGTIYSS 435

Query: 352 SLFPNRAPDGRVLLLNYIGGATNTEILSKTESELVEVVDRDLRKILINPNTEDPLSLSVR 411
           SLFP RAP GR L+LNYIGG TN  IL KT+ EL E VDRDLR+ILINPN + P  L VR
Sbjct: 436 SLFPGRAPPGRTLILNYIGGDTNPGILDKTKDELAEAVDRDLRRILINPNAKAPRVLGVR 495

Query: 412 VWPQAIPQFLIGHLDLLDTAKAGLRDAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVAG 471
           VWPQAIPQFLIGH DLLD AKA L D G +GLFLGGNYV GVALGRC+EGAYE+AAEV  
Sbjct: 496 VWPQAIPQFLIGHFDLLDAAKAALTDGGHKGLFLGGNYVSGVALGRCIEGAYESAAEVVD 555

Query: 472 FLSK 476
           FLS+
Sbjct: 556 FLSQ 558

BLAST of HG10009638 vs. ExPASy Swiss-Prot
Match: P56601 (Coproporphyrinogen III oxidase OS=Myxococcus xanthus OX=34 GN=cgoX PE=1 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 5.5e-41
Identity = 121/393 (30.79%), Postives = 194/393 (49.36%), Query Frame = 0

Query: 81  DPDAP-RFVLWNGKLRPVPGKPTDLPFFDLMSLGGKIRAGFGALGIRPPPPGREESVEEF 140
           DP A  R+V   G+LR VP  P      D++ LG ++R   G L  R  P G +ES+  F
Sbjct: 89  DPAAKRRYVYTRGRLRSVPASPPAFLASDILPLGARLRVA-GELFSRRAPEGVDESLAAF 148

Query: 141 VRRNLGNEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWRLEQNGGSIIGGTFKAIQERN 200
            RR+LG+   + L++   +G+YAGD  +LS+ A F  + ++E+   S+I G  +A + + 
Sbjct: 149 GRRHLGHRATQVLLDAVQTGIYAGDVEQLSVAATFPMLVKMEREHRSLILGAIRAQKAQR 208

Query: 201 KATKPPRDPRLPKPKGQTVGSFRKGLAMLPNAISTCLGSKVKLSWKLSSISKVDDGGYSL 260
           +A  P      PK  G  + +F  GL +L +A++  LG    +  ++  +++ +DGG+ L
Sbjct: 209 QAALPA--GTAPKLSG-ALSTFDGGLQVLIDALAASLGDAAHVGARVEGLAR-EDGGWRL 268

Query: 261 TYETPEGLVSILSRSIIMTVPSYIAGTLLRPISGKAADALSKFYYPPVASVTISYPKGAI 320
             E       +    +++  P++    LLRP+    A  ++   Y P+A V + +  G +
Sbjct: 269 IIEEHGRRAELSVAQVVLAAPAHATAKLLRPLDDALAALVAGIAYAPIAVVHLGFDAGTL 328

Query: 321 RKECLIDGELKGFGQLHPRSQGVTTLGTIYSSSLFPNRAPDGRVLLLNYIGGATNTEILS 380
                      GFG L P  +    LG I++S+ FP RA  GRVL    +GGA    ++ 
Sbjct: 329 PAP-------DGFGFLVPAEEQRRMLGAIHASTTFPFRAEGGRVLYSCMVGGARQPGLVE 388

Query: 381 KTESELVEVVDRDLRKILINPNTEDPLSLSVRVWPQAIPQFLIGHLDLLDTAKAGLRDAG 440
           + E  L  +   +L+ +     T  P    V  WP  IPQ+ +GHL+ +    A L+   
Sbjct: 389 QDEDALAALAREELKALA--GVTARPSFTRVFRWPLGIPQYNLGHLERVAAIDAALQ--R 448

Query: 441 MEGLFLGGNYVCGVALGRCVEGAYEAA-AEVAG 472
           + GL L GN   GV L  C+  A + A A VAG
Sbjct: 449 LPGLHLIGNAYKGVGLNDCIRNAAQLADALVAG 465

BLAST of HG10009638 vs. ExPASy TrEMBL
Match: A0A0A0KMZ2 (Protoporphyrinogen oxidase OS=Cucumis sativus OX=3659 GN=Csa_5G128250 PE=3 SV=1)

HSP 1 Score: 890.2 bits (2299), Expect = 3.9e-255
Identity = 455/549 (82.88%), Postives = 467/549 (85.06%), Query Frame = 0

Query: 1   MATGATLLTDLPFRRPHPLTLLRPSNIPSYHPLHISLRNNHLRPNFRCSIAEGSTALSPS 60
           MATG+TLLTDLPFRRPHPLTLLRPSNIPS+HPLHISL+NN LR  FRCSIAEGSTALSPS
Sbjct: 1   MATGSTLLTDLPFRRPHPLTLLRPSNIPSFHPLHISLQNNRLRSYFRCSIAEGSTALSPS 60

Query: 61  KASGQSS----------------------------------------------------- 120
            AS QSS                                                     
Sbjct: 61  NASSQSSILDCVVVGAGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYL 120

Query: 121 -----------------MVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLM 180
                            +VDSGLKDDLVLGDPDAPRFVLWNGKLRPVP KP DLPFFDLM
Sbjct: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLM 180

Query: 181 SLGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSM 240
           S+GGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSM
Sbjct: 181 SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSM 240

Query: 241 KAAFGKVWRLEQNGGSIIGGTFKAIQERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPN 300
           KAAFGKVWRLEQNGGSIIGGTFKA+QERNK TKPPRDPRLPKPKGQTVGSFRKGL MLPN
Sbjct: 241 KAAFGKVWRLEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPN 300

Query: 301 AISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLVSILSRSIIMTVPSYIAGTLLRP 360
           AISTCLGSKVK+SWKLSSISKVDDGGYSLTYETPEGLVSILSRS+IMTVPSYIAGTLLRP
Sbjct: 301 AISTCLGSKVKVSWKLSSISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYIAGTLLRP 360

Query: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420
           ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS
Sbjct: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420

Query: 421 SSLFPNRAPDGRVLLLNYIGGATNTEILSKTESELVEVVDRDLRKILINPNTEDPLSLSV 480
           SSLFPNRAPDGRVLLLNYIGGATNT ILS+TESEL+EVVDRDLRKILINPN EDPL LSV
Sbjct: 421 SSLFPNRAPDGRVLLLNYIGGATNTGILSQTESELIEVVDRDLRKILINPNAEDPLPLSV 480

BLAST of HG10009638 vs. ExPASy TrEMBL
Match: A0A5D3BGI3 (Protoporphyrinogen oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00240 PE=3 SV=1)

HSP 1 Score: 887.5 bits (2292), Expect = 2.5e-254
Identity = 454/549 (82.70%), Postives = 467/549 (85.06%), Query Frame = 0

Query: 1   MATGATLLTDLPFRRPHPLTLLRPSNIPSYHPLHISLRNNHLRPNFRCSIAEGSTALSPS 60
           MATGATLLTDLPFRRPHPLTLLRPS+IPS++PLHISL+NN LRP+FRCSIAEGSTALSPS
Sbjct: 1   MATGATLLTDLPFRRPHPLTLLRPSDIPSFYPLHISLQNNRLRPHFRCSIAEGSTALSPS 60

Query: 61  KASGQSS----------------------------------------------------- 120
            AS QSS                                                     
Sbjct: 61  NASSQSSILDCVVVGGGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYL 120

Query: 121 -----------------MVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLM 180
                            +VDSGLKDDLVLGDPDAPRFVLWNGKLRPVP KP DLPFFDLM
Sbjct: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLM 180

Query: 181 SLGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSM 240
           S+GGKIRAGFGALGIRPPPPGREESVEEFVRRNLG+EVFERLIEPFCSGVYAGDPSKLSM
Sbjct: 181 SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM 240

Query: 241 KAAFGKVWRLEQNGGSIIGGTFKAIQERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPN 300
           KAAFGKVWRLEQNGGSIIGGTFKA+QERNK TKPPRDPRLPKPKGQTVGSFRKGL MLPN
Sbjct: 241 KAAFGKVWRLEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPN 300

Query: 301 AISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLVSILSRSIIMTVPSYIAGTLLRP 360
           AISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLV ILSRS+IMTVPSYIAGTLLRP
Sbjct: 301 AISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLVPILSRSVIMTVPSYIAGTLLRP 360

Query: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420
           ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS
Sbjct: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420

Query: 421 SSLFPNRAPDGRVLLLNYIGGATNTEILSKTESELVEVVDRDLRKILINPNTEDPLSLSV 480
           SSLFPNRAPDGRVLLLNYIGGATNT ILS+ ESEL+EVVDRDLRKILINPN EDPL LSV
Sbjct: 421 SSLFPNRAPDGRVLLLNYIGGATNTGILSQKESELIEVVDRDLRKILINPNAEDPLPLSV 480

BLAST of HG10009638 vs. ExPASy TrEMBL
Match: A0A1S3CQR2 (Protoporphyrinogen oxidase OS=Cucumis melo OX=3656 GN=LOC103503703 PE=3 SV=1)

HSP 1 Score: 884.4 bits (2284), Expect = 2.1e-253
Identity = 453/549 (82.51%), Postives = 466/549 (84.88%), Query Frame = 0

Query: 1   MATGATLLTDLPFRRPHPLTLLRPSNIPSYHPLHISLRNNHLRPNFRCSIAEGSTALSPS 60
           MATGATLLTDLPFRRPHPLTLLRPS+IPS++PLHISL+NN LR +FRCSIAEGSTALSPS
Sbjct: 1   MATGATLLTDLPFRRPHPLTLLRPSDIPSFYPLHISLQNNRLRSHFRCSIAEGSTALSPS 60

Query: 61  KASGQSS----------------------------------------------------- 120
            AS QSS                                                     
Sbjct: 61  NASSQSSILDCVVVGGGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYL 120

Query: 121 -----------------MVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLM 180
                            +VDSGLKDDLVLGDPDAPRFVLWNGKLRPVP KP DLPFFDLM
Sbjct: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLM 180

Query: 181 SLGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSM 240
           S+GGKIRAGFGALGIRPPPPGREESVEEFVRRNLG+EVFERLIEPFCSGVYAGDPSKLSM
Sbjct: 181 SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM 240

Query: 241 KAAFGKVWRLEQNGGSIIGGTFKAIQERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPN 300
           KAAFGKVWRLEQNGGSIIGGTFKA+QERNK TKPPRDPRLPKPKGQTVGSFRKGL MLPN
Sbjct: 241 KAAFGKVWRLEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPN 300

Query: 301 AISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLVSILSRSIIMTVPSYIAGTLLRP 360
           AISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLV ILSRS+IMTVPSYIAGTLLRP
Sbjct: 301 AISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLVPILSRSVIMTVPSYIAGTLLRP 360

Query: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420
           ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS
Sbjct: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420

Query: 421 SSLFPNRAPDGRVLLLNYIGGATNTEILSKTESELVEVVDRDLRKILINPNTEDPLSLSV 480
           SSLFPNRAPDGRVLLLNYIGGATNT ILS+ ESEL+EVVDRDLRKILINPN EDPL LSV
Sbjct: 421 SSLFPNRAPDGRVLLLNYIGGATNTGILSQKESELIEVVDRDLRKILINPNAEDPLPLSV 480

BLAST of HG10009638 vs. ExPASy TrEMBL
Match: A0A6J1JZ95 (Protoporphyrinogen oxidase OS=Cucurbita maxima OX=3661 GN=LOC111489213 PE=3 SV=1)

HSP 1 Score: 858.6 bits (2217), Expect = 1.2e-245
Identity = 440/549 (80.15%), Postives = 456/549 (83.06%), Query Frame = 0

Query: 1   MATGATLLTDLPFRRPHPLTLLRPSNIPSYHPLHISLRNNHLRPNFRCSIAEGSTALSPS 60
           MA+GATLLTDLPFRRP+P TL RPSNIPS+HPLHIS+R NHLRPNFRCSIAEGS  +S S
Sbjct: 1   MASGATLLTDLPFRRPYPPTLFRPSNIPSFHPLHISIRINHLRPNFRCSIAEGSATVSTS 60

Query: 61  KASGQSS----------------------------------------------------- 120
             SGQSS                                                     
Sbjct: 61  DVSGQSSRVDCVVVGGGISGLCIAQALATRHPDVAPNIIVTEAKARVGGNITTVERDGYL 120

Query: 121 -----------------MVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLM 180
                            +VDSGLKDDLVLGDPDAPRFVLW GKLRPVP KP DLPFFDLM
Sbjct: 121 WEEGPNSFQPSDPLLTMVVDSGLKDDLVLGDPDAPRFVLWKGKLRPVPAKPNDLPFFDLM 180

Query: 181 SLGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSM 240
           SLGGKIRAGFGALGIRPPPPGREESVEEFVRRNLG+EVFERLIEPFCSGVYAGDPSKLSM
Sbjct: 181 SLGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM 240

Query: 241 KAAFGKVWRLEQNGGSIIGGTFKAIQERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPN 300
           KAAFGKVW+LEQNGGSIIGGTFKA QERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPN
Sbjct: 241 KAAFGKVWKLEQNGGSIIGGTFKAFQERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPN 300

Query: 301 AISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLVSILSRSIIMTVPSYIAGTLLRP 360
           AISTCLG+KVKLSWKLS+ISKVDDGGYSLTYETPEGLVSILSRS+IMTVPSY+A TLLRP
Sbjct: 301 AISTCLGNKVKLSWKLSNISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYVASTLLRP 360

Query: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420
           IS KAAD LS+FYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS
Sbjct: 361 ISVKAADVLSEFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420

Query: 421 SSLFPNRAPDGRVLLLNYIGGATNTEILSKTESELVEVVDRDLRKILINPNTEDPLSLSV 480
           SSLFPNRAPDGRVLLLNYIGGATNTEILSKTESELVEVVDRDLRKILIN N EDP+ LSV
Sbjct: 421 SSLFPNRAPDGRVLLLNYIGGATNTEILSKTESELVEVVDRDLRKILINTNAEDPMLLSV 480

BLAST of HG10009638 vs. ExPASy TrEMBL
Match: A0A6J1FEL7 (Protoporphyrinogen oxidase OS=Cucurbita moschata OX=3662 GN=LOC111444800 PE=3 SV=1)

HSP 1 Score: 854.4 bits (2206), Expect = 2.3e-244
Identity = 439/549 (79.96%), Postives = 456/549 (83.06%), Query Frame = 0

Query: 1   MATGATLLTDLPFRRPHPLTLLRPSNIPSYHPLHISLRNNHLRPNFRCSIAEGSTALSPS 60
           MA+GATLLTDLPFRRP+P TL RPSNIPS+HPLHIS+R NHLRPNFRCSIAEGST +S S
Sbjct: 1   MASGATLLTDLPFRRPYPPTLFRPSNIPSFHPLHISIRINHLRPNFRCSIAEGSTTVSTS 60

Query: 61  KASGQSS----------------------------------------------------- 120
             SGQSS                                                     
Sbjct: 61  DVSGQSSRVDCVVVGGGISGLCIAQALATKHPDVAPNIIVTEAKARVGGNITTVERDGYL 120

Query: 121 -----------------MVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLM 180
                            +VDSGLKDDLVLGDPDAPRFVLW GKLRPVP KP DLPFFDLM
Sbjct: 121 WEEGPNSFQPSDPLLTMVVDSGLKDDLVLGDPDAPRFVLWKGKLRPVPAKPNDLPFFDLM 180

Query: 181 SLGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSM 240
           SLGGKIRAGFGALGIRPPPPGREESVEEFVRRNLG+EVFERLIEPFCSGVYAGD SKLSM
Sbjct: 181 SLGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDSSKLSM 240

Query: 241 KAAFGKVWRLEQNGGSIIGGTFKAIQERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPN 300
           KAAFGKVW+LEQNGGSIIGGTFKA QERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPN
Sbjct: 241 KAAFGKVWKLEQNGGSIIGGTFKAFQERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPN 300

Query: 301 AISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLVSILSRSIIMTVPSYIAGTLLRP 360
           AISTCLG+KVKLSWKLS+ISKVDDG YSLTYETPEGLVSILSRS+IMTVPSY+A TLLRP
Sbjct: 301 AISTCLGNKVKLSWKLSNISKVDDGSYSLTYETPEGLVSILSRSVIMTVPSYVASTLLRP 360

Query: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420
           IS KAADALSKFYYPPVASVTISYPKGAIRKECLI GELKGFGQLHPRSQGVTTLGTIYS
Sbjct: 361 ISVKAADALSKFYYPPVASVTISYPKGAIRKECLIGGELKGFGQLHPRSQGVTTLGTIYS 420

Query: 421 SSLFPNRAPDGRVLLLNYIGGATNTEILSKTESELVEVVDRDLRKILINPNTEDPLSLSV 480
           SSLFPNRAPDGRVLLLNYIGGATNTEILSKTES+LVEVVD+DLRKILIN N EDP+ LSV
Sbjct: 421 SSLFPNRAPDGRVLLLNYIGGATNTEILSKTESKLVEVVDQDLRKILINTNAEDPMLLSV 480

BLAST of HG10009638 vs. TAIR 10
Match: AT4G01690.1 (Flavin containing amine oxidoreductase family )

HSP 1 Score: 691.4 bits (1783), Expect = 5.1e-199
Identity = 340/428 (79.44%), Postives = 374/428 (87.38%), Query Frame = 0

Query: 52  EGSTALSPSKASGQSSMVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLMS 111
           EG  +  PS     + +VDSGLKDDLVLGDP APRFVLWNGKLRPVP K TDLPFFDLMS
Sbjct: 111 EGPNSFQPSDPM-LTMVVDSGLKDDLVLGDPTAPRFVLWNGKLRPVPSKLTDLPFFDLMS 170

Query: 112 LGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSMK 171
           +GGKIRAGFGALGIRP PPGREESVEEFVRRNLG+EVFERLIEPFCSGVYAGDPSKLSMK
Sbjct: 171 IGGKIRAGFGALGIRPSPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMK 230

Query: 172 AAFGKVWRLEQNGGSIIGGTFKAIQERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPNA 231
           AAFGKVW+LEQNGGSIIGGTFKAIQER  A K  RDPRLPKP+GQTVGSFRKGL MLP A
Sbjct: 231 AAFGKVWKLEQNGGSIIGGTFKAIQERKNAPKAERDPRLPKPQGQTVGSFRKGLRMLPEA 290

Query: 232 ISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLVSILSRSIIMTVPSYIAGTLLRPI 291
           IS  LGSKVKLSWKLS I+K++ GGY+LTYETP+GLVS+ S+S++MTVPS++A  LLRP+
Sbjct: 291 ISARLGSKVKLSWKLSGITKLESGGYNLTYETPDGLVSVQSKSVVMTVPSHVASGLLRPL 350

Query: 292 SGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSS 351
           S  AA+ALSK YYPPVA+V+ISYPK AIR ECLIDGELKGFGQLHPR+QGV TLGTIYSS
Sbjct: 351 SESAANALSKLYYPPVAAVSISYPKEAIRTECLIDGELKGFGQLHPRTQGVETLGTIYSS 410

Query: 352 SLFPNRAPDGRVLLLNYIGGATNTEILSKTESELVEVVDRDLRKILINPNTEDPLSLSVR 411
           SLFPNRAP GR+LLLNYIGG+TNT ILSK+E ELVE VDRDLRK+LI PN+ DPL L VR
Sbjct: 411 SLFPNRAPPGRILLLNYIGGSTNTGILSKSEGELVEAVDRDLRKMLIKPNSTDPLKLGVR 470

Query: 412 VWPQAIPQFLIGHLDLLDTAKAGLRDAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVAG 471
           VWPQAIPQFL+GH D+LDTAK+ L  +G EGLFLGGNYV GVALGRCVEGAYE A EV  
Sbjct: 471 VWPQAIPQFLVGHFDILDTAKSSLTSSGYEGLFLGGNYVAGVALGRCVEGAYETAIEVNN 530

Query: 472 FLSKKVYK 480
           F+S+  YK
Sbjct: 531 FMSRYAYK 537

BLAST of HG10009638 vs. TAIR 10
Match: AT4G01690.2 (Flavin containing amine oxidoreductase family )

HSP 1 Score: 623.2 bits (1606), Expect = 1.7e-178
Identity = 315/428 (73.60%), Postives = 347/428 (81.07%), Query Frame = 0

Query: 52  EGSTALSPSKASGQSSMVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLMS 111
           EG  +  PS     + +VDSGLKDDLVLGDP APRFVLWNGKLRPVP K TDLPFFDLMS
Sbjct: 111 EGPNSFQPSDPM-LTMVVDSGLKDDLVLGDPTAPRFVLWNGKLRPVPSKLTDLPFFDLMS 170

Query: 112 LGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSMK 171
           +GGKIRAGFGALGIRP PPGREESVEEFVRRNLG+EVFERLIEPFCSGVYAGDPSKLSMK
Sbjct: 171 IGGKIRAGFGALGIRPSPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMK 230

Query: 172 AAFGKVWRLEQNGGSIIGGTFKAIQERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPNA 231
           AAFGKVW+LEQNGGSIIGGTFKAIQER  A K  RDPRLPKP+GQTVGSFRKGL MLP A
Sbjct: 231 AAFGKVWKLEQNGGSIIGGTFKAIQERKNAPKAERDPRLPKPQGQTVGSFRKGLRMLPEA 290

Query: 232 ISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLVSILSRSIIMTVPSYIAGTLLRPI 291
           IS  LGSKVKLSWKLS I+K++ GGY+LTYETP+GLVS+ S+S++MTVPS++A  LLRP+
Sbjct: 291 ISARLGSKVKLSWKLSGITKLESGGYNLTYETPDGLVSVQSKSVVMTVPSHVASGLLRPL 350

Query: 292 SGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSS 351
           S  AA+ALSK YYPPVA+V+ISYPK AIR ECLIDGELKGFGQLHPR+QGV TLGTIYSS
Sbjct: 351 SESAANALSKLYYPPVAAVSISYPKEAIRTECLIDGELKGFGQLHPRTQGVETLGTIYSS 410

Query: 352 SLFPNRAPDGRVLLLNYIGGATNTEILSKTESELVEVVDRDLRKILINPNTEDPLSLSVR 411
           SLFPNRAP GR+LLLNYIGG+TNT ILSK+E ELVE                        
Sbjct: 411 SLFPNRAPPGRILLLNYIGGSTNTGILSKSEGELVEA----------------------- 470

Query: 412 VWPQAIPQFLIGHLDLLDTAKAGLRDAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVAG 471
                   FL+GH D+LDTAK+ L  +G EGLFLGGNYV GVALGRCVEGAYE A EV  
Sbjct: 471 --------FLVGHFDILDTAKSSLTSSGYEGLFLGGNYVAGVALGRCVEGAYETAIEVNN 506

Query: 472 FLSKKVYK 480
           F+S+  YK
Sbjct: 531 FMSRYAYK 506

BLAST of HG10009638 vs. TAIR 10
Match: AT5G14220.1 (Flavin containing amine oxidoreductase family )

HSP 1 Score: 140.2 bits (352), Expect = 4.3e-33
Identity = 118/441 (26.76%), Postives = 205/441 (46.49%), Query Frame = 0

Query: 52  EGSTALSPSKASGQSSMVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLMS 111
           EG+  ++ ++    S + D GL++          R+++ NG    +P  P +L    ++S
Sbjct: 67  EGANTMTEAEPEVGSLLDDLGLREKQQFPISQKKRYIVRNGVPVMLPTNPIELVTSSVLS 126

Query: 112 LGGKIRAGFGAL-----GIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPS 171
              K +             +      EESV EF +R+ G EV + LI+PF  G  A DP 
Sbjct: 127 TQSKFQILLEPFLWKKKSSKVSDASAEESVSEFFQRHFGQEVVDYLIDPFVGGTSAADPD 186

Query: 172 KLSMKAAFGKVWRLEQNGGSIIGGTFKAIQERNKATKPPRDPRLPKPKGQTVG--SFRKG 231
            LSMK +F  +W +E++ GSII G  +  +   K  K       P  K  + G  SF+ G
Sbjct: 187 SLSMKHSFPDLWNVEKSFGSIIVGAIRT-KFAAKGGKSRDTKSSPGTKKGSRGSFSFKGG 246

Query: 232 LAMLPNAISTCLG-------SKVKLSWKLSSISKVDDGGYSLTYETPEGLVSILSRSIIM 291
           + +LP+ +   L        SKV LS   +S S+ ++   S          +    ++IM
Sbjct: 247 MQILPDTLCKSLSHDEINLDSKV-LSLSYNSGSRQENWSLSCVSHNETQRQNPHYDAVIM 306

Query: 292 TVPSYIAGTLLRPISGK--AADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQL 351
           T P      +     G+    + L +  Y P++ +  ++ K  +++       L+GFG L
Sbjct: 307 TAPLCNVKEMKVMKGGQPFQLNFLPEINYMPLSVLITTFTKEKVKR------PLEGFGVL 366

Query: 352 HP---RSQGVTTLGTIYSSSLFPNRAPDGRVLLLNYIGGATNTEILSKTESELVEVVDRD 411
            P   +  G  TLGT++SS +FP+R+P    L   +IGG+ N E+   +  EL +VV  D
Sbjct: 367 IPSKEQKHGFKTLGTLFSSMMFPDRSPSDVHLYTTFIGGSRNQELAKASTDELKQVVTSD 426

Query: 412 LRKILINPNTEDPLSLSVRVWPQAIPQFLIGHLDLLDTAKAGLRDAGMEGLFLGGNYVCG 471
           L+++L      +P+S++   W +A P +   +  +++       D  + G F  GN+  G
Sbjct: 427 LQRLL--GVEGEPVSVNHYYWRKAFPLYDSSYDSVMEAIDKMEND--LPGFFYAGNHRGG 486

Query: 472 VALGRCVEGAYEAAAEVAGFL 474
           +++G+ +    +AA  V  +L
Sbjct: 487 LSVGKSIASGCKAADLVISYL 495

BLAST of HG10009638 vs. TAIR 10
Match: AT5G14220.2 (Flavin containing amine oxidoreductase family )

HSP 1 Score: 132.5 bits (332), Expect = 9.0e-31
Identity = 104/355 (29.30%), Postives = 174/355 (49.01%), Query Frame = 0

Query: 133 EESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWRLEQNGGSIIGGTF 192
           EESV EF +R+ G EV + LI+PF  G  A DP  LSMK +F  +W +E++ GSII G  
Sbjct: 123 EESVSEFFQRHFGQEVVDYLIDPFVGGTSAADPDSLSMKHSFPDLWNVEKSFGSIIVGAI 182

Query: 193 KAIQERNKATKPPRDPRLPKPKGQTVG--SFRKGLAMLPNAISTCLG-------SKVKLS 252
           +  +   K  K       P  K  + G  SF+ G+ +LP+ +   L        SKV LS
Sbjct: 183 RT-KFAAKGGKSRDTKSSPGTKKGSRGSFSFKGGMQILPDTLCKSLSHDEINLDSKV-LS 242

Query: 253 WKLSSISKVDDGGYSLTYETPEGLVSILSRSIIMTVPSYIAGTLLRPISGK--AADALSK 312
              +S S+ ++   S          +    ++IMT P      +     G+    + L +
Sbjct: 243 LSYNSGSRQENWSLSCVSHNETQRQNPHYDAVIMTAPLCNVKEMKVMKGGQPFQLNFLPE 302

Query: 313 FYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHP---RSQGVTTLGTIYSSSLFPNRA 372
             Y P++ +  ++ K  +++       L+GFG L P   +  G  TLGT++SS +FP+R+
Sbjct: 303 INYMPLSVLITTFTKEKVKR------PLEGFGVLIPSKEQKHGFKTLGTLFSSMMFPDRS 362

Query: 373 PDGRVLLLNYIGGATNTEILSKTESELVEVVDRDLRKILINPNTEDPLSLSVRVWPQAIP 432
           P    L   +IGG+ N E+   +  EL +VV  DL+++L      +P+S++   W +A P
Sbjct: 363 PSDVHLYTTFIGGSRNQELAKASTDELKQVVTSDLQRLL--GVEGEPVSVNHYYWRKAFP 422

Query: 433 QFLIGHLDLLDTAKAGLRDAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVAGFL 474
            +   +  +++       D  + G F  GN+  G+++G+ +    +AA  V  +L
Sbjct: 423 LYDSSYDSVMEAIDKMEND--LPGFFYAGNHRGGLSVGKSIASGCKAADLVISYL 465