Homology
BLAST of HG10009635 vs. NCBI nr
Match:
XP_038874652.1 (proline--tRNA ligase, cytoplasmic isoform X1 [Benincasa hispida])
HSP 1 Score: 959.1 bits (2478), Expect = 1.7e-275
Identity = 486/590 (82.37%), Postives = 493/590 (83.56%), Query Frame = 0
Query: 12 MAGPKPGSSTSNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 71
MAGPKPGSST+NPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1 MAGPKPGSSTTNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
Query: 72 YILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 131
YILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61 YILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
Query: 132 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLL 191
SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIR
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
Query: 192 LLVLIFLLLSFHYVSKHNKLVDLFDVTRKCCGVFKGNCEGVDLLGLLRCRVTTHHRTNAY 251
L
Sbjct: 181 FL---------------------------------------------------------- 240
Query: 252 YNYKIYYLGQIWRFKHAKLNCKTHFDWKQKVLEILELYRRIYEEYLAIPVIKGKKSEMEK 311
W+ H K D +VLEILELYRRIYEEYLAIPVIKGKKSEMEK
Sbjct: 241 -----------WQEGHTAFATKDEAD--TEVLEILELYRRIYEEYLAIPVIKGKKSEMEK 300
Query: 312 FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST 371
FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST
Sbjct: 301 FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST 360
Query: 372 RTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTRGILDACSATLDTLSKAGIRA 431
RTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADT+GI DACSATLDTLSKAGIRA
Sbjct: 361 RTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRA 420
Query: 432 EVDIRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQV 491
EVD RDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQV
Sbjct: 421 EVDSRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQV 480
Query: 492 KELLESIQQSLFDAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEDDIVVFLQEV 551
KELL+SIQQSLFDAAKEKRDACI+VINTWEEFTEALSQKKMILAPWCDEE EV
Sbjct: 481 KELLDSIQQSLFDAAKEKRDACIKVINTWEEFTEALSQKKMILAPWCDEE--------EV 511
Query: 552 EKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 602
EKDVKTRTKGEMGAAKTLCSPF+QP LPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 541 EKDVKTRTKGEMGAAKTLCSPFQQPDLPEGTKCFASGKPAKKWSYWGRSY 511
BLAST of HG10009635 vs. NCBI nr
Match:
KAG7032618.1 (Proline--tRNA ligase, cytoplasmic [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 956.1 bits (2470), Expect = 1.5e-274
Identity = 482/590 (81.69%), Postives = 492/590 (83.39%), Query Frame = 0
Query: 12 MAGPKPGSSTSNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 71
MAGPKPGSS PKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1 MAGPKPGSSAKTPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
Query: 72 YILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 131
YILRPWA+S+WETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61 YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
Query: 132 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLL 191
SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQ+L
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQVL 180
Query: 192 LLVLIFLLLSFHYVSKHNKLVDLFDVTRKCCGVFKGNCEGVDLLGLLRCRVTTHHRTNAY 251
V F F
Sbjct: 181 FRVFFFSSREF------------------------------------------------- 240
Query: 252 YNYKIYYLGQIWRFKHAKLNCKTHFDWKQKVLEILELYRRIYEEYLAIPVIKGKKSEMEK 311
+W+ H K D +VLEILELYRRIYEEYLAIPVIKGKKSEMEK
Sbjct: 241 ----------LWQEGHTAFATKDEAD--TEVLEILELYRRIYEEYLAIPVIKGKKSEMEK 300
Query: 312 FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST 371
FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST
Sbjct: 301 FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST 360
Query: 372 RTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTRGILDACSATLDTLSKAGIRA 431
RTIGVMVMVHGDDKGLVLPPKVASVQV+IVPVPYKDADT+GI DACS TLDTLS+AGIRA
Sbjct: 361 RTIGVMVMVHGDDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSVTLDTLSEAGIRA 420
Query: 432 EVDIRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQV 491
EVD RDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNS KKDIPRASLVEQV
Sbjct: 421 EVDARDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRASLVEQV 480
Query: 492 KELLESIQQSLFDAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEDDIVVFLQEV 551
KELL+SIQQSLFDAAK KRD CIQVINTWEEFTEALSQKKMILAPWCDEE EV
Sbjct: 481 KELLDSIQQSLFDAAKVKRDTCIQVINTWEEFTEALSQKKMILAPWCDEE--------EV 521
Query: 552 EKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 602
EKDVKTRTKGEMGAAKTLCSPFEQP LPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 541 EKDVKTRTKGEMGAAKTLCSPFEQPELPEGTKCFASGKPAKKWSYWGRSY 521
BLAST of HG10009635 vs. NCBI nr
Match:
KAA0044600.1 (proline--tRNA ligase [Cucumis melo var. makuwa])
HSP 1 Score: 952.6 bits (2461), Expect = 1.6e-273
Identity = 482/590 (81.69%), Postives = 492/590 (83.39%), Query Frame = 0
Query: 12 MAGPKPGSSTSNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 71
MAGPKPGSS +N KAGGKKKEVKKETGLGLTNKKD+NFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1 MAGPKPGSSATNQKAGGKKKEVKKETGLGLTNKKDENFGEWYSEVVVSGEMIEYYDISGC 60
Query: 72 YILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 131
YILRPWAIS+WETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61 YILRPWAISIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
Query: 132 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLL 191
SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLL
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLL 180
Query: 192 LLVLIFLLLSFHYVSKHNKLVDLFDVTRKCCGVFKGNCEGVDLLGLLRCRVTTHHRTNAY 251
+L L
Sbjct: 181 MLFL-------------------------------------------------------- 240
Query: 252 YNYKIYYLGQIWRFKHAKLNCKTHFDWKQKVLEILELYRRIYEEYLAIPVIKGKKSEMEK 311
W+ H K D +VLEILELYRRIYEEYLAIPVIKGKKSEMEK
Sbjct: 241 -----------WQEGHTAFATKDEAD--TEVLEILELYRRIYEEYLAIPVIKGKKSEMEK 300
Query: 312 FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST 371
FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFEN+KGEKAMVWQNSWAYST
Sbjct: 301 FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENDKGEKAMVWQNSWAYST 360
Query: 372 RTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTRGILDACSATLDTLSKAGIRA 431
RTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADT+GI DACSATLDTL+ AGIRA
Sbjct: 361 RTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLTDAGIRA 420
Query: 432 EVDIRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQV 491
EVD RDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNS KKDIPR SLVEQV
Sbjct: 421 EVDSRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRDSLVEQV 480
Query: 492 KELLESIQQSLFDAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEDDIVVFLQEV 551
KELLESIQQSLFDAAK KRD CIQVINTWEEFTEALSQKKMILAPWCDEE EV
Sbjct: 481 KELLESIQQSLFDAAKVKRDTCIQVINTWEEFTEALSQKKMILAPWCDEE--------EV 513
Query: 552 EKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 602
EKDVKTRTKGEMGAAKTLCSPF+QPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 541 EKDVKTRTKGEMGAAKTLCSPFDQPALPEGTKCFASGKPAKKWSYWGRSY 513
BLAST of HG10009635 vs. NCBI nr
Match:
XP_038907011.1 (proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida] >XP_038907020.1 proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida] >XP_038907029.1 proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida])
HSP 1 Score: 952.6 bits (2461), Expect = 1.6e-273
Identity = 483/590 (81.86%), Postives = 489/590 (82.88%), Query Frame = 0
Query: 12 MAGPKPGSSTSNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 71
MAGPKPGSS +NPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1 MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
Query: 72 YILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 131
YILRPWAIS+WETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61 YILRPWAISIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
Query: 132 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLL 191
SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIR
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
Query: 192 LLVLIFLLLSFHYVSKHNKLVDLFDVTRKCCGVFKGNCEGVDLLGLLRCRVTTHHRTNAY 251
L
Sbjct: 181 FL---------------------------------------------------------- 240
Query: 252 YNYKIYYLGQIWRFKHAKLNCKTHFDWKQKVLEILELYRRIYEEYLAIPVIKGKKSEMEK 311
W+ H K D +VLEILELYR IYEEYLAIPVIKGKKSEMEK
Sbjct: 241 -----------WQEGHTAFATKDEAD--TEVLEILELYRCIYEEYLAIPVIKGKKSEMEK 300
Query: 312 FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST 371
FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST
Sbjct: 301 FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST 360
Query: 372 RTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTRGILDACSATLDTLSKAGIRA 431
RTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADT+GI DACSATLDTLS+AGIRA
Sbjct: 361 RTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSEAGIRA 420
Query: 432 EVDIRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQV 491
EVD RDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPR SLVEQV
Sbjct: 421 EVDSRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRDSLVEQV 480
Query: 492 KELLESIQQSLFDAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEDDIVVFLQEV 551
KELLESIQQSLFDAAKEKRD CIQVINTWEEFTEAL QKKMILAPWCDEE EV
Sbjct: 481 KELLESIQQSLFDAAKEKRDKCIQVINTWEEFTEALGQKKMILAPWCDEE--------EV 511
Query: 552 EKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 602
EKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 541 EKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 511
BLAST of HG10009635 vs. NCBI nr
Match:
XP_022979761.1 (proline--tRNA ligase, cytoplasmic-like [Cucurbita maxima])
HSP 1 Score: 949.5 bits (2453), Expect = 1.4e-272
Identity = 483/590 (81.86%), Postives = 490/590 (83.05%), Query Frame = 0
Query: 12 MAGPKPGSSTSNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 71
MAGPKPGSS + PKAGG KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1 MAGPKPGSSATKPKAGG-KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
Query: 72 YILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 131
YILRPWA+S+WETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61 YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
Query: 132 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLL 191
SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIR
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
Query: 192 LLVLIFLLLSFHYVSKHNKLVDLFDVTRKCCGVFKGNCEGVDLLGLLRCRVTTHHRTNAY 251
L
Sbjct: 181 FL---------------------------------------------------------- 240
Query: 252 YNYKIYYLGQIWRFKHAKLNCKTHFDWKQKVLEILELYRRIYEEYLAIPVIKGKKSEMEK 311
W+ H K D +VLEILELYRRIYEEYLAIPVIKGKKSEMEK
Sbjct: 241 -----------WQEGHTAFATKDEAD--AEVLEILELYRRIYEEYLAIPVIKGKKSEMEK 300
Query: 312 FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST 371
FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST
Sbjct: 301 FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST 360
Query: 372 RTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTRGILDACSATLDTLSKAGIRA 431
RTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADT+GI DACSATLDTLSKAGIRA
Sbjct: 361 RTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRA 420
Query: 432 EVDIRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQV 491
EVD RDNYSPGWKYSHWEMKGVPLRIEIGPKDLA+NQVRAVRRDNSGKKDIPRASLVEQV
Sbjct: 421 EVDSRDNYSPGWKYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLVEQV 480
Query: 492 KELLESIQQSLFDAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEDDIVVFLQEV 551
KELLESIQQSLFDAAKEKRD CIQVINTWEEFTEALSQKKMILAPWCDEE EV
Sbjct: 481 KELLESIQQSLFDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEE--------EV 510
Query: 552 EKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 602
EKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 541 EKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 510
BLAST of HG10009635 vs. ExPASy Swiss-Prot
Match:
Q9M1R2 (Proline--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g62120 PE=1 SV=1)
HSP 1 Score: 814.7 bits (2103), Expect = 7.0e-235
Identity = 403/597 (67.50%), Postives = 457/597 (76.55%), Query Frame = 0
Query: 5 QKSKTETMAGPKPGSSTSNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIE 64
+K K E + K ++S +G KKK+VKKETGLGL+ KKD+NFGEWYSE V +MIE
Sbjct: 17 EKVKKEKVVKEKVAKASS---SGQKKKDVKKETGLGLSVKKDENFGEWYSE-VCKQDMIE 76
Query: 65 YYDISGCYILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAP 124
YYDISGCYILRPW++++WE MQ+FFDAEIKKMK+KNCYFPLFVSPGVL++EKDHIEGFAP
Sbjct: 77 YYDISGCYILRPWSMAIWEIMQIFFDAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAP 136
Query: 125 EVAWVTKSGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPT 184
EVAWVTKSG+SDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFS+PT
Sbjct: 137 EVAWVTKSGKSDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPT 196
Query: 185 PFIRQLLLLVLIFLLLSFHYVSKHNKLVDLFDVTRKCCGVFKGNCEGVDLLGLLRCRVTT 244
PFIR L
Sbjct: 197 PFIRSREFL--------------------------------------------------- 256
Query: 245 HHRTNAYYNYKIYYLGQIWRFKHAKLNCKTHFDWKQKVLEILELYRRIYEEYLAIPVIKG 304
W+ H K D ++VL+ILELYRRIYEEYLA+PV+KG
Sbjct: 257 ------------------WQEGHTAFATKAEAD--EEVLQILELYRRIYEEYLAVPVVKG 316
Query: 305 KKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQ 364
KSE EKFAGGLYTTSVEAFIPNTGRG+QGATSHCLGQNFAKMFEINFENEK E MVWQ
Sbjct: 317 MKSENEKFAGGLYTTSVEAFIPNTGRGVQGATSHCLGQNFAKMFEINFENEKAETEMVWQ 376
Query: 365 NSWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTRGILDACSATLDTL 424
NSWAYSTRTIGVM+M HGDDKGLVLPPKVASVQV+++PVPYKDA+T+GI DAC+AT L
Sbjct: 377 NSWAYSTRTIGVMIMTHGDDKGLVLPPKVASVQVVVIPVPYKDANTQGIYDACTATASAL 436
Query: 425 SKAGIRAEVDIRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPR 484
+AGIRAE D+RDNYSPGWKYS WEMKGVPLRIEIGP+DL N+QVR VRRDN K+DIPR
Sbjct: 437 CEAGIRAEEDLRDNYSPGWKYSDWEMKGVPLRIEIGPRDLENDQVRTVRRDNGVKEDIPR 496
Query: 485 ASLVEQVKELLESIQQSLFDAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEDDI 544
SLVE VKELLE IQQ++++ AK+KR+AC+Q + TW+EF +AL++KK+ILAPWCDEE
Sbjct: 497 GSLVEHVKELLEKIQQNMYEVAKQKREACVQEVKTWDEFIKALNEKKLILAPWCDEE--- 530
Query: 545 VVFLQEVEKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 602
EVE+DVK RTKGE GAAKTLCSPF+QP LPEGT CFASGKPAKKW+YWGRSY
Sbjct: 557 -----EVERDVKARTKGETGAAKTLCSPFDQPELPEGTLCFASGKPAKKWTYWGRSY 530
BLAST of HG10009635 vs. ExPASy Swiss-Prot
Match:
O60155 (Putative proline--tRNA ligase C19C7.06 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=prs1 PE=3 SV=1)
HSP 1 Score: 523.1 bits (1346), Expect = 4.2e-147
Identity = 291/615 (47.32%), Postives = 367/615 (59.67%), Query Frame = 0
Query: 17 PGSST--SNPKAGGKKKEVKKETG-------LGLTNKKDDNFGEWYSEVVVSGEMIEYYD 76
PGS+T S P A KK E K +G+T +KD +F WY +V+ +MIEYYD
Sbjct: 174 PGSATAPSKPAAQKKKAEPSKNDAAIENAALIGITVRKDADFPNWYQQVLTKSDMIEYYD 233
Query: 77 ISGCYILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVA 136
ISGCYIL+PW+ S+WE +Q +FD EIKK+ ++N YFPLFVS VL++EKDH+EGFAPEVA
Sbjct: 234 ISGCYILKPWSYSIWEAIQGWFDKEIKKLGVRNGYFPLFVSSKVLEKEKDHVEGFAPEVA 293
Query: 137 WVTKSGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFI 196
WVT++G S+L+ PIAIRPTSETVMYPYY+KWIR HRDLPLKLNQW +VVRWEF +P PF+
Sbjct: 294 WVTRAGTSELDEPIAIRPTSETVMYPYYAKWIRSHRDLPLKLNQWNSVVRWEFKNPQPFL 353
Query: 197 RQLLLLVLIFLLLSFHYVSKHNKLVDLFDVTRKCCGVFKGNCEGVDLLGLLRCRVTTHHR 256
R TR+
Sbjct: 354 R-----------------------------TRE--------------------------- 413
Query: 257 TNAYYNYKIYYLGQIWRFKHAKLNCKTHFDWKQKVLEILELYRRIYEEYLAIPVIKGKKS 316
+L Q H L T ++V +IL+LY RIY + LA+PVIKG KS
Sbjct: 414 ----------FLWQEGHTAHMTLEGAT-----EEVHQILDLYARIYTDLLAVPVIKGVKS 473
Query: 317 EMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGE--------K 376
E EKFAGG++TT+VE +IP TGRGIQGATSHCLGQNF+KMF I E+ E K
Sbjct: 474 ENEKFAGGMFTTTVEGYIPTTGRGIQGATSHCLGQNFSKMFNIVVEDPNAEIGPTGERPK 533
Query: 377 AMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVIIVPV----PYKDADTRGILD 436
VWQNSW STRTIGV VMVHGDDKGL LPP +A VQ ++VP D + I
Sbjct: 534 LFVWQNSWGLSTRTIGVAVMVHGDDKGLKLPPAIALVQSVVVPCGITNKTTDQERNEIEG 593
Query: 437 ACSATLDTLSKAGIRAEVDIRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRD 496
CS D L+ A IR E D+R Y+PG+K+SHWEMKGVPLR+E GP D NQV AVRRD
Sbjct: 594 FCSKLADRLNAADIRTEADLR-AYTPGYKFSHWEMKGVPLRLEYGPNDAKKNQVTAVRRD 653
Query: 497 NSGKKDIPRASLVEQVKELLESIQQSLFDAAKEKRDACIQVINTWEEFTEALSQKKMILA 556
K +P +L + V +LL IQ ++++ AK +RDA + + W +F AL++K +++
Sbjct: 654 TFEKIPVPLNNLEKGVSDLLAKIQTNMYETAKAERDAHVVKVKEWADFVPALNKKNIVMI 713
Query: 557 PWCD----EEDDIVVFLQEVEKDVKTRTKGEMGAAKTLCSPFEQP----ALPEG-TKCFA 602
PWC+ E++ ++V D K AK+LC P EQP A+ EG TKC
Sbjct: 714 PWCNTTECEKEIKKNSARQVNGDEPEDEKAPSMGAKSLCIPLEQPSGEDAIIEGTTKCAG 716
BLAST of HG10009635 vs. ExPASy Swiss-Prot
Match:
P28668 (Bifunctional glutamate/proline--tRNA ligase OS=Drosophila melanogaster OX=7227 GN=GluProRS PE=1 SV=2)
HSP 1 Score: 514.2 bits (1323), Expect = 2.0e-144
Identity = 279/607 (45.96%), Postives = 368/607 (60.63%), Query Frame = 0
Query: 8 KTETMAGPKPGSSTSNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYD 67
K A PKP A VKK+T LGL K+DN +WYS+V+ GEMIEYYD
Sbjct: 1184 KAPKEAQPKPAKPVKKEPAADASGAVKKQTRLGLEATKEDNLPDWYSQVITKGEMIEYYD 1243
Query: 68 ISGCYILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVA 127
+SGCYILR W+ ++W+ ++ +FDAEI +M +K CYFP+FVS VL++EK HI FAPEVA
Sbjct: 1244 VSGCYILRQWSFAIWKAIKTWFDAEITRMGVKECYFPIFVSKAVLEKEKTHIADFAPEVA 1303
Query: 128 WVTKSGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFI 187
WVTKSG+SDL PIA+RPTSETVMYP Y+KW++ +RDLP++LNQW NVVRWEF PTPF+
Sbjct: 1304 WVTKSGDSDLAEPIAVRPTSETVMYPAYAKWVQSYRDLPIRLNQWNNVVRWEFKQPTPFL 1363
Query: 188 RQLLLLVLIFLLLSFHYVSKHNKLVDLFDVTRKCCGVFKGNCEGVDLLGLLRCRVTTHHR 247
R TR+
Sbjct: 1364 R-----------------------------TREF-------------------------- 1423
Query: 248 TNAYYNYKIYYLGQIWRFKHAKLNCKTHFDWKQKVLEILELYRRIYEEYLAIPVIKGKKS 307
+W+ H K + ++VL+IL+LY +Y LAIPV+KG+K+
Sbjct: 1424 --------------LWQEGHTAFADKE--EAAKEVLDILDLYALVYTHLLAIPVVKGRKT 1483
Query: 308 EMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFEN-EKGEKAMVWQNS 367
E EKFAGG YTT+VEAFI +GR IQGATSH LGQNF+KMFEI +E+ E +K V+QNS
Sbjct: 1484 EKEKFAGGDYTTTVEAFISASGRAIQGATSHHLGQNFSKMFEIVYEDPETQQKKYVYQNS 1543
Query: 368 WAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVIIVP----VPYKDADTRGILDACSATLD 427
W +TRTIGVM+MVH D++GLVLPP VA +Q I+VP V KD + +LDAC A
Sbjct: 1544 WGITTRTIGVMIMVHADNQGLVLPPHVACIQAIVVPCGITVNTKDDERAQLLDACKALEK 1603
Query: 428 TLSKAGIRAEVDIRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDI 487
L G+R E D RDNYSPGWK++HWE+KGVPLR+E+GPKDL Q+ AVRRD K I
Sbjct: 1604 RLVGGGVRCEGDYRDNYSPGWKFNHWELKGVPLRLEVGPKDLKAQQLVAVRRDTVEKITI 1663
Query: 488 PRASLVEQVKELLESIQQSLFDAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEED 547
P A + +++ LLE+I +S+ + A+E + + + W +F L QK ++LAP+C E
Sbjct: 1664 PLADVEKKIPALLETIHESMLNKAQEDMTSHTKKVTNWTDFCGFLEQKNILLAPFCGE-- 1714
Query: 548 DIVVFLQEVEKDVKTRTKGEMGA----AKTLCSPFEQPA-LPEGTKCF---ASGKPAKKW 602
+ +++ D + E GA AK+LC PF+QPA + KC + KP K +
Sbjct: 1724 --ISCEDKIKADSARGEEAEPGAPAMGAKSLCIPFDQPAPIAASDKCINPSCTNKP-KFY 1714
BLAST of HG10009635 vs. ExPASy Swiss-Prot
Match:
Q8CGC7 (Bifunctional glutamate/proline--tRNA ligase OS=Mus musculus OX=10090 GN=Eprs1 PE=1 SV=4)
HSP 1 Score: 513.5 bits (1321), Expect = 3.3e-144
Identity = 279/593 (47.05%), Postives = 368/593 (62.06%), Query Frame = 0
Query: 18 GSSTSNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPW 77
GS S+ A G+ + KK+T LGL KK++N EWYS+V+ EMIEYYD+SGCYILRPW
Sbjct: 994 GSGLSSGGA-GEGQGPKKQTRLGLEAKKEENLAEWYSQVITKSEMIEYYDVSGCYILRPW 1053
Query: 78 AISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDL 137
+ S+WE+++ FFDAEIKK+ ++NCYFP+FVS L++EK+HIE FAPEVAWVT+SG+++L
Sbjct: 1054 SYSIWESIKDFFDAEIKKLGVENCYFPIFVSQAALEKEKNHIEDFAPEVAWVTRSGKTEL 1113
Query: 138 EVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLLLLVLIF 197
PIAIRPTSETVMYP Y+KW++ HRDLP++LNQWCNVVRWEF HP PF+R
Sbjct: 1114 AEPIAIRPTSETVMYPAYAKWVQSHRDLPVRLNQWCNVVRWEFKHPQPFLR--------- 1173
Query: 198 LLLSFHYVSKHNKLVDLFDVTRKCCGVFKGNCEGVDLLGLLRCRVTTHHRTNAYYNYKIY 257
TR+
Sbjct: 1174 --------------------TREF------------------------------------ 1233
Query: 258 YLGQIWRFKHAKLNCKTHFDWKQKVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLY 317
+W+ H+ T + +VL+ILELY R+YEE LAIPV++G+K+E EKFAGG Y
Sbjct: 1234 ----LWQEGHSAF--ATFEEAADEVLQILELYARVYEELLAIPVVRGRKTEKEKFAGGDY 1293
Query: 318 TTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEK--GEKAMVWQNSWAYSTRTIG 377
TT++EAFI +GR IQGATSH LGQNF+KM EI FE+ K GEK +Q SW +TRTIG
Sbjct: 1294 TTTIEAFISASGRAIQGATSHHLGQNFSKMCEIVFEDPKTPGEKQFAYQCSWGLTTRTIG 1353
Query: 378 VMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDA----DTRGILDACSATLDTLSKAGIRA 437
VMVMVHGD+ GLVLPP+VASVQV+++P +A D ++ C+ L A IR
Sbjct: 1354 VMVMVHGDNMGLVLPPRVASVQVVVIPCGITNALSEEDREALMAKCNEYRRRLLGANIRV 1413
Query: 438 EVDIRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQV 497
VD+RDNYSPGWK++HWE+KGVP+R+E+GP+D+ + Q AVRRD K I ++
Sbjct: 1414 RVDLRDNYSPGWKFNHWELKGVPVRLEVGPRDMKSCQFVAVRRDTGEKLTIAEKEAEAKL 1473
Query: 498 KELLESIQQSLFDAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEED--DIVVFLQ 557
+++LE IQ +LF A E + V NT E+F + L K+ P+C E D D + +
Sbjct: 1474 EKVLEDIQLNLFTRASEDLKTHMVVSNTLEDFQKVLDAGKVAQIPFCGEIDCEDWIKKMT 1512
Query: 558 EVEKDVKTRTKGEMGAAKTLCSPFEQPA-LPEGTKCFASGKPAKKWSYWGRSY 602
++DV+ MG AK+LC PF L G C PAK ++ +GRSY
Sbjct: 1534 ARDQDVEPGAP-SMG-AKSLCIPFNPLCELQPGAMCVCGKNPAKFYTLFGRSY 1512
BLAST of HG10009635 vs. ExPASy Swiss-Prot
Match:
P07814 (Bifunctional glutamate/proline--tRNA ligase OS=Homo sapiens OX=9606 GN=EPRS1 PE=1 SV=5)
HSP 1 Score: 511.5 bits (1316), Expect = 1.3e-143
Identity = 266/581 (45.78%), Postives = 353/581 (60.76%), Query Frame = 0
Query: 28 GKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWAISVWETMQV 87
G+ + KK+T LGL KK++N +WYS+V+ EMIEY+DISGCYILRPWA ++WE ++
Sbjct: 1003 GEGQGPKKQTRLGLEAKKEENLADWYSQVITKSEMIEYHDISGCYILRPWAYAIWEAIKD 1062
Query: 88 FFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLEVPIAIRPTS 147
FFDAEIKK+ ++NCYFP+FVS L++EK H+ FAPEVAWVT+SG+++L PIAIRPTS
Sbjct: 1063 FFDAEIKKLGVENCYFPMFVSQSALEKEKTHVADFAPEVAWVTRSGKTELAEPIAIRPTS 1122
Query: 148 ETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLLLLVLIFLLLSFHYVSK 207
ETVMYP Y+KW++ HRDLP+KLNQWCNVVRWEF HP PF+R
Sbjct: 1123 ETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLR------------------- 1182
Query: 208 HNKLVDLFDVTRKCCGVFKGNCEGVDLLGLLRCRVTTHHRTNAYYNYKIYYLGQIWRFKH 267
TR+ +W+ H
Sbjct: 1183 ----------TREF----------------------------------------LWQEGH 1242
Query: 268 AKLNCKTHFDWKQKVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPN 327
+ T + ++VL+IL+LY ++YEE LAIPV+KG+K+E EKFAGG YTT++EAFI
Sbjct: 1243 SAF--ATMEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTIEAFISA 1302
Query: 328 TGRGIQGATSHCLGQNFAKMFEINFENEK--GEKAMVWQNSWAYSTRTIGVMVMVHGDDK 387
+GR IQG TSH LGQNF+KMFEI FE+ K GEK +QNSW +TRTIGVM MVHGD+
Sbjct: 1303 SGRAIQGGTSHHLGQNFSKMFEIVFEDPKIPGEKQFAYQNSWGLTTRTIGVMTMVHGDNM 1362
Query: 388 GLVLPPKVASVQVIIVPVPYKDA----DTRGILDACSATLDTLSKAGIRAEVDIRDNYSP 447
GLVLPP+VA VQV+I+P +A D ++ C+ L IR D+RDNYSP
Sbjct: 1363 GLVLPPRVACVQVVIIPCGITNALSEEDKEALIAKCNDYRRRLLSVNIRVRADLRDNYSP 1422
Query: 448 GWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQVKELLESIQQS 507
GWK++HWE+KGVP+R+E+GP+D+ + Q AVRRD K + +++ +LE IQ +
Sbjct: 1423 GWKFNHWELKGVPIRLEVGPRDMKSCQFVAVRRDTGEKLTVAENEAETKLQAILEDIQVT 1482
Query: 508 LFDAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEDDIVVFLQEVEKDVKTRTKG 567
LF A E + V NT E+F + L K++ P+C E D + +D
Sbjct: 1483 LFTRASEDLKTHMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTARDQDLEPGA 1512
Query: 568 EMGAAKTLCSPFEQPA-LPEGTKCFASGKPAKKWSYWGRSY 602
AK+LC PF+ L G KC PAK ++ +GRSY
Sbjct: 1543 PSMGAKSLCIPFKPLCELQPGAKCVCGKNPAKYYTLFGRSY 1512
BLAST of HG10009635 vs. ExPASy TrEMBL
Match:
A0A5A7TN35 (Prolyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003510 PE=3 SV=1)
HSP 1 Score: 952.6 bits (2461), Expect = 7.9e-274
Identity = 482/590 (81.69%), Postives = 492/590 (83.39%), Query Frame = 0
Query: 12 MAGPKPGSSTSNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 71
MAGPKPGSS +N KAGGKKKEVKKETGLGLTNKKD+NFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1 MAGPKPGSSATNQKAGGKKKEVKKETGLGLTNKKDENFGEWYSEVVVSGEMIEYYDISGC 60
Query: 72 YILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 131
YILRPWAIS+WETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61 YILRPWAISIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
Query: 132 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLL 191
SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLL
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLL 180
Query: 192 LLVLIFLLLSFHYVSKHNKLVDLFDVTRKCCGVFKGNCEGVDLLGLLRCRVTTHHRTNAY 251
+L L
Sbjct: 181 MLFL-------------------------------------------------------- 240
Query: 252 YNYKIYYLGQIWRFKHAKLNCKTHFDWKQKVLEILELYRRIYEEYLAIPVIKGKKSEMEK 311
W+ H K D +VLEILELYRRIYEEYLAIPVIKGKKSEMEK
Sbjct: 241 -----------WQEGHTAFATKDEAD--TEVLEILELYRRIYEEYLAIPVIKGKKSEMEK 300
Query: 312 FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST 371
FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFEN+KGEKAMVWQNSWAYST
Sbjct: 301 FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENDKGEKAMVWQNSWAYST 360
Query: 372 RTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTRGILDACSATLDTLSKAGIRA 431
RTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADT+GI DACSATLDTL+ AGIRA
Sbjct: 361 RTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLTDAGIRA 420
Query: 432 EVDIRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQV 491
EVD RDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNS KKDIPR SLVEQV
Sbjct: 421 EVDSRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRDSLVEQV 480
Query: 492 KELLESIQQSLFDAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEDDIVVFLQEV 551
KELLESIQQSLFDAAK KRD CIQVINTWEEFTEALSQKKMILAPWCDEE EV
Sbjct: 481 KELLESIQQSLFDAAKVKRDTCIQVINTWEEFTEALSQKKMILAPWCDEE--------EV 513
Query: 552 EKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 602
EKDVKTRTKGEMGAAKTLCSPF+QPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 541 EKDVKTRTKGEMGAAKTLCSPFDQPALPEGTKCFASGKPAKKWSYWGRSY 513
BLAST of HG10009635 vs. ExPASy TrEMBL
Match:
A0A6J1IRP0 (Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111479369 PE=3 SV=1)
HSP 1 Score: 949.5 bits (2453), Expect = 6.7e-273
Identity = 483/590 (81.86%), Postives = 490/590 (83.05%), Query Frame = 0
Query: 12 MAGPKPGSSTSNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 71
MAGPKPGSS + PKAGG KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1 MAGPKPGSSATKPKAGG-KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
Query: 72 YILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 131
YILRPWA+S+WETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61 YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
Query: 132 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLL 191
SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIR
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
Query: 192 LLVLIFLLLSFHYVSKHNKLVDLFDVTRKCCGVFKGNCEGVDLLGLLRCRVTTHHRTNAY 251
L
Sbjct: 181 FL---------------------------------------------------------- 240
Query: 252 YNYKIYYLGQIWRFKHAKLNCKTHFDWKQKVLEILELYRRIYEEYLAIPVIKGKKSEMEK 311
W+ H K D +VLEILELYRRIYEEYLAIPVIKGKKSEMEK
Sbjct: 241 -----------WQEGHTAFATKDEAD--AEVLEILELYRRIYEEYLAIPVIKGKKSEMEK 300
Query: 312 FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST 371
FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST
Sbjct: 301 FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST 360
Query: 372 RTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTRGILDACSATLDTLSKAGIRA 431
RTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADT+GI DACSATLDTLSKAGIRA
Sbjct: 361 RTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRA 420
Query: 432 EVDIRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQV 491
EVD RDNYSPGWKYSHWEMKGVPLRIEIGPKDLA+NQVRAVRRDNSGKKDIPRASLVEQV
Sbjct: 421 EVDSRDNYSPGWKYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLVEQV 480
Query: 492 KELLESIQQSLFDAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEDDIVVFLQEV 551
KELLESIQQSLFDAAKEKRD CIQVINTWEEFTEALSQKKMILAPWCDEE EV
Sbjct: 481 KELLESIQQSLFDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEE--------EV 510
Query: 552 EKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 602
EKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 541 EKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 510
BLAST of HG10009635 vs. ExPASy TrEMBL
Match:
A0A6J1GUJ5 (Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111457586 PE=3 SV=1)
HSP 1 Score: 947.2 bits (2447), Expect = 3.3e-272
Identity = 481/590 (81.53%), Postives = 489/590 (82.88%), Query Frame = 0
Query: 12 MAGPKPGSSTSNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 71
MAGPKPGSS + PKAGG KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1 MAGPKPGSSATKPKAGG-KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
Query: 72 YILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 131
YILRPWA+S+WETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61 YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
Query: 132 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLL 191
SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIR
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
Query: 192 LLVLIFLLLSFHYVSKHNKLVDLFDVTRKCCGVFKGNCEGVDLLGLLRCRVTTHHRTNAY 251
L
Sbjct: 181 FL---------------------------------------------------------- 240
Query: 252 YNYKIYYLGQIWRFKHAKLNCKTHFDWKQKVLEILELYRRIYEEYLAIPVIKGKKSEMEK 311
W+ H K D +VLEILELYRRIYEEYLAIPVIKGKKSEMEK
Sbjct: 241 -----------WQEGHTAFATKDEAD--TEVLEILELYRRIYEEYLAIPVIKGKKSEMEK 300
Query: 312 FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST 371
FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST
Sbjct: 301 FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST 360
Query: 372 RTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTRGILDACSATLDTLSKAGIRA 431
RTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADT+GI DACS TLDTLS+AGIRA
Sbjct: 361 RTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAGIRA 420
Query: 432 EVDIRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQV 491
EVD RDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRA+LVEQV
Sbjct: 421 EVDSRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQV 480
Query: 492 KELLESIQQSLFDAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEDDIVVFLQEV 551
KELLESIQQSLFDAAKEKRD CIQVINTWEEFTEALSQKKMILAPWCDEE EV
Sbjct: 481 KELLESIQQSLFDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEE--------EV 510
Query: 552 EKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 602
EKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 541 EKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 510
BLAST of HG10009635 vs. ExPASy TrEMBL
Match:
A0A1S4DS87 (Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503756 PE=3 SV=1)
HSP 1 Score: 946.8 bits (2446), Expect = 4.3e-272
Identity = 479/590 (81.19%), Postives = 487/590 (82.54%), Query Frame = 0
Query: 12 MAGPKPGSSTSNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 71
MAGPKPGSS +NPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1 MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
Query: 72 YILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 131
YILRPWA+SVWETMQ FFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61 YILRPWAMSVWETMQEFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
Query: 132 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLL 191
SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIR
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
Query: 192 LLVLIFLLLSFHYVSKHNKLVDLFDVTRKCCGVFKGNCEGVDLLGLLRCRVTTHHRTNAY 251
L
Sbjct: 181 FL---------------------------------------------------------- 240
Query: 252 YNYKIYYLGQIWRFKHAKLNCKTHFDWKQKVLEILELYRRIYEEYLAIPVIKGKKSEMEK 311
W+ H K D +VLEILELYRRIYEEYLAIPVIKGKKSEMEK
Sbjct: 241 -----------WQEGHTAFATKDEAD--TEVLEILELYRRIYEEYLAIPVIKGKKSEMEK 300
Query: 312 FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST 371
FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST
Sbjct: 301 FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST 360
Query: 372 RTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTRGILDACSATLDTLSKAGIRA 431
RTIGVMVMVHGDDKGLV+PPKVASVQVIIVPVPYKDADTRGI DACSAT D LSKAGIRA
Sbjct: 361 RTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDACSATSDALSKAGIRA 420
Query: 432 EVDIRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQV 491
EVDIR+NYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNS KKDIPRA LVEQV
Sbjct: 421 EVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLVEQV 480
Query: 492 KELLESIQQSLFDAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEDDIVVFLQEV 551
KELLESIQQSLFDAAKEKRDACIQV+NTWEEFTEAL QKKMILAPWC+EE EV
Sbjct: 481 KELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEE--------EV 511
Query: 552 EKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 602
EKDVKTRTKGEMGAAKTLCSPFEQP LPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 541 EKDVKTRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 511
BLAST of HG10009635 vs. ExPASy TrEMBL
Match:
A0A6J1GYJ1 (Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111458443 PE=3 SV=1)
HSP 1 Score: 946.4 bits (2445), Expect = 5.7e-272
Identity = 478/590 (81.02%), Postives = 486/590 (82.37%), Query Frame = 0
Query: 12 MAGPKPGSSTSNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 71
MAGPKPGSS PKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1 MAGPKPGSSAKTPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
Query: 72 YILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 131
YILRPWA+S+WETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61 YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
Query: 132 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLL 191
SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIR
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
Query: 192 LLVLIFLLLSFHYVSKHNKLVDLFDVTRKCCGVFKGNCEGVDLLGLLRCRVTTHHRTNAY 251
L
Sbjct: 181 FL---------------------------------------------------------- 240
Query: 252 YNYKIYYLGQIWRFKHAKLNCKTHFDWKQKVLEILELYRRIYEEYLAIPVIKGKKSEMEK 311
W+ H K D +VLEILELYRRIYEEYLAIPVIKGKKSEMEK
Sbjct: 241 -----------WQEGHTAFATKDEAD--TEVLEILELYRRIYEEYLAIPVIKGKKSEMEK 300
Query: 312 FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST 371
FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST
Sbjct: 301 FAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYST 360
Query: 372 RTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTRGILDACSATLDTLSKAGIRA 431
RTIGVMVMVHGDDKGLVLPPKVASVQV+IVPVPYKDADT+GI DACS TLDTLS+AGIRA
Sbjct: 361 RTIGVMVMVHGDDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSVTLDTLSEAGIRA 420
Query: 432 EVDIRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQV 491
EVD RDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNS KKDIPRASLVEQV
Sbjct: 421 EVDARDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRASLVEQV 480
Query: 492 KELLESIQQSLFDAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEDDIVVFLQEV 551
KELL+SIQQSLFDAAK KRD CIQVINTWEEFTEALSQKKMILAPWCDEE EV
Sbjct: 481 KELLDSIQQSLFDAAKVKRDTCIQVINTWEEFTEALSQKKMILAPWCDEE--------EV 511
Query: 552 EKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 602
EKDVKTRTKGEMGAAKTLCSPFEQP LPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 541 EKDVKTRTKGEMGAAKTLCSPFEQPELPEGTKCFASGKPAKKWSYWGRSY 511
BLAST of HG10009635 vs. TAIR 10
Match:
AT3G62120.1 (Class II aaRS and biotin synthetases superfamily protein )
HSP 1 Score: 814.7 bits (2103), Expect = 5.0e-236
Identity = 403/597 (67.50%), Postives = 457/597 (76.55%), Query Frame = 0
Query: 5 QKSKTETMAGPKPGSSTSNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIE 64
+K K E + K ++S +G KKK+VKKETGLGL+ KKD+NFGEWYSE V +MIE
Sbjct: 17 EKVKKEKVVKEKVAKASS---SGQKKKDVKKETGLGLSVKKDENFGEWYSE-VCKQDMIE 76
Query: 65 YYDISGCYILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAP 124
YYDISGCYILRPW++++WE MQ+FFDAEIKKMK+KNCYFPLFVSPGVL++EKDHIEGFAP
Sbjct: 77 YYDISGCYILRPWSMAIWEIMQIFFDAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAP 136
Query: 125 EVAWVTKSGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPT 184
EVAWVTKSG+SDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFS+PT
Sbjct: 137 EVAWVTKSGKSDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPT 196
Query: 185 PFIRQLLLLVLIFLLLSFHYVSKHNKLVDLFDVTRKCCGVFKGNCEGVDLLGLLRCRVTT 244
PFIR L
Sbjct: 197 PFIRSREFL--------------------------------------------------- 256
Query: 245 HHRTNAYYNYKIYYLGQIWRFKHAKLNCKTHFDWKQKVLEILELYRRIYEEYLAIPVIKG 304
W+ H K D ++VL+ILELYRRIYEEYLA+PV+KG
Sbjct: 257 ------------------WQEGHTAFATKAEAD--EEVLQILELYRRIYEEYLAVPVVKG 316
Query: 305 KKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQ 364
KSE EKFAGGLYTTSVEAFIPNTGRG+QGATSHCLGQNFAKMFEINFENEK E MVWQ
Sbjct: 317 MKSENEKFAGGLYTTSVEAFIPNTGRGVQGATSHCLGQNFAKMFEINFENEKAETEMVWQ 376
Query: 365 NSWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTRGILDACSATLDTL 424
NSWAYSTRTIGVM+M HGDDKGLVLPPKVASVQV+++PVPYKDA+T+GI DAC+AT L
Sbjct: 377 NSWAYSTRTIGVMIMTHGDDKGLVLPPKVASVQVVVIPVPYKDANTQGIYDACTATASAL 436
Query: 425 SKAGIRAEVDIRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPR 484
+AGIRAE D+RDNYSPGWKYS WEMKGVPLRIEIGP+DL N+QVR VRRDN K+DIPR
Sbjct: 437 CEAGIRAEEDLRDNYSPGWKYSDWEMKGVPLRIEIGPRDLENDQVRTVRRDNGVKEDIPR 496
Query: 485 ASLVEQVKELLESIQQSLFDAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEDDI 544
SLVE VKELLE IQQ++++ AK+KR+AC+Q + TW+EF +AL++KK+ILAPWCDEE
Sbjct: 497 GSLVEHVKELLEKIQQNMYEVAKQKREACVQEVKTWDEFIKALNEKKLILAPWCDEE--- 530
Query: 545 VVFLQEVEKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 602
EVE+DVK RTKGE GAAKTLCSPF+QP LPEGT CFASGKPAKKW+YWGRSY
Sbjct: 557 -----EVERDVKARTKGETGAAKTLCSPFDQPELPEGTLCFASGKPAKKWTYWGRSY 530
BLAST of HG10009635 vs. TAIR 10
Match:
AT3G62120.2 (Class II aaRS and biotin synthetases superfamily protein )
HSP 1 Score: 814.7 bits (2103), Expect = 5.0e-236
Identity = 403/597 (67.50%), Postives = 457/597 (76.55%), Query Frame = 0
Query: 5 QKSKTETMAGPKPGSSTSNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIE 64
+K K E + K ++S +G KKK+VKKETGLGL+ KKD+NFGEWYSE V +MIE
Sbjct: 17 EKVKKEKVVKEKVAKASS---SGQKKKDVKKETGLGLSVKKDENFGEWYSE-VCKQDMIE 76
Query: 65 YYDISGCYILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAP 124
YYDISGCYILRPW++++WE MQ+FFDAEIKKMK+KNCYFPLFVSPGVL++EKDHIEGFAP
Sbjct: 77 YYDISGCYILRPWSMAIWEIMQIFFDAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAP 136
Query: 125 EVAWVTKSGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPT 184
EVAWVTKSG+SDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFS+PT
Sbjct: 137 EVAWVTKSGKSDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPT 196
Query: 185 PFIRQLLLLVLIFLLLSFHYVSKHNKLVDLFDVTRKCCGVFKGNCEGVDLLGLLRCRVTT 244
PFIR L
Sbjct: 197 PFIRSREFL--------------------------------------------------- 256
Query: 245 HHRTNAYYNYKIYYLGQIWRFKHAKLNCKTHFDWKQKVLEILELYRRIYEEYLAIPVIKG 304
W+ H K D ++VL+ILELYRRIYEEYLA+PV+KG
Sbjct: 257 ------------------WQEGHTAFATKAEAD--EEVLQILELYRRIYEEYLAVPVVKG 316
Query: 305 KKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQ 364
KSE EKFAGGLYTTSVEAFIPNTGRG+QGATSHCLGQNFAKMFEINFENEK E MVWQ
Sbjct: 317 MKSENEKFAGGLYTTSVEAFIPNTGRGVQGATSHCLGQNFAKMFEINFENEKAETEMVWQ 376
Query: 365 NSWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTRGILDACSATLDTL 424
NSWAYSTRTIGVM+M HGDDKGLVLPPKVASVQV+++PVPYKDA+T+GI DAC+AT L
Sbjct: 377 NSWAYSTRTIGVMIMTHGDDKGLVLPPKVASVQVVVIPVPYKDANTQGIYDACTATASAL 436
Query: 425 SKAGIRAEVDIRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPR 484
+AGIRAE D+RDNYSPGWKYS WEMKGVPLRIEIGP+DL N+QVR VRRDN K+DIPR
Sbjct: 437 CEAGIRAEEDLRDNYSPGWKYSDWEMKGVPLRIEIGPRDLENDQVRTVRRDNGVKEDIPR 496
Query: 485 ASLVEQVKELLESIQQSLFDAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEDDI 544
SLVE VKELLE IQQ++++ AK+KR+AC+Q + TW+EF +AL++KK+ILAPWCDEE
Sbjct: 497 GSLVEHVKELLEKIQQNMYEVAKQKREACVQEVKTWDEFIKALNEKKLILAPWCDEE--- 530
Query: 545 VVFLQEVEKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 602
EVE+DVK RTKGE GAAKTLCSPF+QP LPEGT CFASGKPAKKW+YWGRSY
Sbjct: 557 -----EVERDVKARTKGETGAAKTLCSPFDQPELPEGTLCFASGKPAKKWTYWGRSY 530
BLAST of HG10009635 vs. TAIR 10
Match:
AT5G52520.1 (Class II aaRS and biotin synthetases superfamily protein )
HSP 1 Score: 359.8 bits (922), Expect = 4.4e-99
Identity = 207/590 (35.08%), Postives = 310/590 (52.54%), Query Frame = 0
Query: 19 SSTSNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWA 78
S T++P+ K EV + + +F WY +V+ S E+ +Y + G ++RP+
Sbjct: 45 SGTASPET--KSSEVDRLRSDRAVTPRSQDFNAWYLDVIASAELADYGPVRGTMVIRPYG 104
Query: 79 ISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLE 138
++WE +Q + + + K+ N YFP F+ +++E H+EGF+PE+A VT G +LE
Sbjct: 105 YAIWEAIQDYLNVKFKETGHSNMYFPQFIPYSFIEKEASHVEGFSPELALVTVGGGKELE 164
Query: 139 VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLLLLVLIFL 198
+ +RPTSET++ +++WI +RDLPL +NQW NV RWE PFIR L
Sbjct: 165 EKLVVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEM-RTKPFIRTL-------- 224
Query: 199 LLSFHYVSKHNKLVDLFDVTRKCCGVFKGNCEGVDLLGLLRCRVTTHHRTNAYYNYKIYY 258
+
Sbjct: 225 ----------------------------------------------------------EF 284
Query: 259 LGQIWRFKHAKLNCKTHFDWKQKVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYT 318
L Q HA T + +++ +++E+Y R E AIPVI G+KS++E FAG T
Sbjct: 285 LWQEGHTAHA-----TPEEAEKEAKQMIEIYTRFAFEQTAIPVIPGRKSKLETFAGADIT 344
Query: 319 TSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMV 378
++EA + + + +Q TSH LGQNF++ F F +E GE+ VWQ SWA STR +G ++
Sbjct: 345 YTIEAMMGDR-KALQAGTSHNLGQNFSRAFGTQFADENGERQHVWQTSWAVSTRFVGGII 404
Query: 379 MVHGDDKGLVLPPKVASVQVIIVPVPYKDADTRGILDACSATLDTLSKAGIRAEVDIRDN 438
M HGDD GL+LPPK+A +QV+IVP+ KD + G+L A S+ + L AG+R ++D D
Sbjct: 405 MTHGDDTGLMLPPKIAPIQVVIVPIWKKDTEKTGVLSAASSVKEALQTAGVRVKLDDTDQ 464
Query: 439 YSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKK------DIPRASLVEQVK 498
+PGWK++ WEMKG+PLRIEIGP+D+++N V RRD GK + ++LV VK
Sbjct: 465 RTPGWKFNFWEMKGIPLRIEIGPRDVSSNSVVVSRRDVPGKAGKVFGISMEPSTLVAYVK 524
Query: 499 ELLESIQQSLFDAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEDDIVVFLQEVE 558
E L+ IQ SL + A RD+ I +N++ E +A+S K PW + D
Sbjct: 525 EKLDEIQTSLLEKALSFRDSNIVDVNSYAELKDAISSGKWARGPWSASDAD--------- 543
Query: 559 KDVKTRTKGEMGAAKTLCSPFEQPALPEGTK-CFASGKPAKKWSYWGRSY 602
+ R K E GA C PFEQ +GTK C +G PA++ + + +SY
Sbjct: 585 ---EQRVKEETGAT-IRCFPFEQ---TQGTKTCLMTGNPAEEVAIFAKSY 543
BLAST of HG10009635 vs. TAIR 10
Match:
AT5G10880.1 (tRNA synthetase-related / tRNA ligase-related )
HSP 1 Score: 287.3 bits (734), Expect = 2.8e-77
Identity = 145/229 (63.32%), Postives = 167/229 (72.93%), Query Frame = 0
Query: 379 MVHGDDKGLVLPPKVASVQVIIVPVPYKD-ADTRGILDACSATLDTLSKAGIRAEVDIRD 438
M HGDDKGLV PPKVA VQV+++ VP K AD + + DAC A TL AGIRAE DIRD
Sbjct: 89 MTHGDDKGLVFPPKVAPVQVVVIHVPIKGAADYQELCDACEAVESTLLGAGIRAEADIRD 148
Query: 439 NYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQVKELLES 498
NYS GWKY+ E+ GVPLRIE GP+DLAN+QVR V RDN K D+ R L+EQVK+LLE
Sbjct: 149 NYSCGWKYADQELTGVPLRIETGPRDLANDQVRIVTRDNGAKMDVKRGDLIEQVKDLLEK 208
Query: 499 IQQSLFDAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEDDIVVFLQEVEKDVKT 558
IQ +L+D AK K + C Q + TW+EF EALSQKK+ILAPWCD+ EVEKDVK
Sbjct: 209 IQSNLYDVAKRKVEECTQKVETWDEFVEALSQKKLILAPWCDK--------VEVEKDVKR 268
Query: 559 RTKGEM-----GAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 602
RT+G+ G AKTLC+P EQP L E T CFASGKPAKKWSYWGRSY
Sbjct: 269 RTRGDETGGGGGGAKTLCTPLEQPELGEETLCFASGKPAKKWSYWGRSY 309
HSP 2 Score: 63.2 bits (152), Expect = 8.5e-10
Identity = 28/47 (59.57%), Postives = 37/47 (78.72%), Query Frame = 0
Query: 35 KETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYD-ISGCYILRPWAIS 81
KET LG+T KKD++FG+WYSE GE++EYY+ + GCYIL+P IS
Sbjct: 3 KETRLGITAKKDEDFGKWYSEACRFGELVEYYESVKGCYILKPSGIS 49
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_038874652.1 | 1.7e-275 | 82.37 | proline--tRNA ligase, cytoplasmic isoform X1 [Benincasa hispida] | [more] |
| KAG7032618.1 | 1.5e-274 | 81.69 | Proline--tRNA ligase, cytoplasmic [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
| KAA0044600.1 | 1.6e-273 | 81.69 | proline--tRNA ligase [Cucumis melo var. makuwa] | [more] |
| XP_038907011.1 | 1.6e-273 | 81.86 | proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida] >XP_038907... | [more] |
| XP_022979761.1 | 1.4e-272 | 81.86 | proline--tRNA ligase, cytoplasmic-like [Cucurbita maxima] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9M1R2 | 7.0e-235 | 67.50 | Proline--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g62120 P... | [more] |
| O60155 | 4.2e-147 | 47.32 | Putative proline--tRNA ligase C19C7.06 OS=Schizosaccharomyces pombe (strain 972 ... | [more] |
| P28668 | 2.0e-144 | 45.96 | Bifunctional glutamate/proline--tRNA ligase OS=Drosophila melanogaster OX=7227 G... | [more] |
| Q8CGC7 | 3.3e-144 | 47.05 | Bifunctional glutamate/proline--tRNA ligase OS=Mus musculus OX=10090 GN=Eprs1 PE... | [more] |
| P07814 | 1.3e-143 | 45.78 | Bifunctional glutamate/proline--tRNA ligase OS=Homo sapiens OX=9606 GN=EPRS1 PE=... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A5A7TN35 | 7.9e-274 | 81.69 | Prolyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold4... | [more] |
| A0A6J1IRP0 | 6.7e-273 | 81.86 | Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111479369 PE=3 SV=1 | [more] |
| A0A6J1GUJ5 | 3.3e-272 | 81.53 | Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111457586 PE=3 SV=1 | [more] |
| A0A1S4DS87 | 4.3e-272 | 81.19 | Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503756 PE=3 SV=1 | [more] |
| A0A6J1GYJ1 | 5.7e-272 | 81.02 | Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111458443 PE=3 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT3G62120.1 | 5.0e-236 | 67.50 | Class II aaRS and biotin synthetases superfamily protein | [more] |
| AT3G62120.2 | 5.0e-236 | 67.50 | Class II aaRS and biotin synthetases superfamily protein | [more] |
| AT5G52520.1 | 4.4e-99 | 35.08 | Class II aaRS and biotin synthetases superfamily protein | [more] |
| AT5G10880.1 | 2.8e-77 | 63.32 | tRNA synthetase-related / tRNA ligase-related | [more] |
