HG10009633 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10009633 vs. NCBI nr
Match: XP_038875889.1 (pectinesterase-like [Benincasa hispida])

HSP 1 Score: 1016.1 bits (2626), Expect = 1.1e-292
Identity = 513/568 (90.32%), Postives = 543/568 (95.60%), Query Frame = 0

Query: 1   MVGKVVVSGLSLILVVGVALAV---VNKSSSSNAAAEDLSPKMKAISTICSTADYQQECK 60
           MVGKVVVSGLSLILVVGVALAV   VNKS+SSNAA E  SPKMKAISTICST+DYQ+ECK
Sbjct: 1   MVGKVVVSGLSLILVVGVALAVVATVNKSNSSNAALE--SPKMKAISTICSTSDYQEECK 60

Query: 61  NTLSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDL 120
           NTLSNVAHNA+SN PK+Y KAAVLAT+EEITKGYNFSD+LMVEA NNA+ KMSVEDCKDL
Sbjct: 61  NTLSNVAHNATSNDPKDYAKAAVLATIEEITKGYNFSDTLMVEAANNASTKMSVEDCKDL 120

Query: 121 LQFAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQ 180
           LQFAIDELQ SYSA+GDSDLHTD DRVADI NWLSAVISYQQSCLDGLEEF+PQLK+KMQ
Sbjct: 121 LQFAIDELQESYSAIGDSDLHTDTDRVADINNWLSAVISYQQSCLDGLEEFNPQLKQKMQ 180

Query: 181 DNLGVAAKLTSNALAIVSAVSNMLENYGLQLK-VQPSGRKLLGTTEVGSDGFPTWLSGAD 240
           DNL VA+KLTSNALA+VSAVSN+L+NY LQLK VQP+GR+LLGTTEVGSDGFP WLSGAD
Sbjct: 181 DNLSVASKLTSNALAVVSAVSNILDNYRLQLKQVQPNGRRLLGTTEVGSDGFPIWLSGAD 240

Query: 241 RKLLAARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITV 300
           RKLLAARG GG+ARPNAVVAKDGSGQYKSIAAALAAYPK LKGRYVIYVKAGIYDE+I V
Sbjct: 241 RKLLAARGRGGRARPNAVVAKDGSGQYKSIAAALAAYPKALKGRYVIYVKAGIYDEHIIV 300

Query: 301 TKDMRNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGH 360
           TKDM+NVFMYGDGPRKTIVTGRK+NRDG TTQNTASF+ALG+GFLCKSMGF+NTAGPEGH
Sbjct: 301 TKDMKNVFMYGDGPRKTIVTGRKNNRDGFTTQNTASFAALGNGFLCKSMGFTNTAGPEGH 360

Query: 361 QAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLI 420
           QAVALRVQSDRSAFFNCRMDGHQDTLY QTHRQFYRNCVISGTVDFIFGDS+T+IQNSLI
Sbjct: 361 QAVALRVQSDRSAFFNCRMDGHQDTLYAQTHRQFYRNCVISGTVDFIFGDSTTLIQNSLI 420

Query: 421 IVRRPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYAR 480
           IVRRPMDNQQNTVTAQGR  QK+ITGLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYAR
Sbjct: 421 IVRRPMDNQQNTVTAQGRDLQKQITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAR 480

Query: 481 TVIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEA 540
           TVIME+TLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEA
Sbjct: 481 TVIMETTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEA 540

Query: 541 LQYTAGPFLLGRAWLPTTGGFYFLGLKN 565
           LQYTAGPFLLGRAWLP+TGG YFLGLKN
Sbjct: 541 LQYTAGPFLLGRAWLPSTGGPYFLGLKN 566

BLAST of HG10009633 vs. NCBI nr
Match: KAA0050274.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYJ98133.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1001.9 bits (2589), Expect = 2.2e-288
Identity = 496/567 (87.48%), Postives = 535/567 (94.36%), Query Frame = 0

Query: 1   MVGKVVVSGLSLILVVGVALAVV---NKSSSSNAAAEDLSPKMKAISTICSTADYQQECK 60
           MVGKV+VS LSLILVVG+ALAVV   NK + SN   E++SPKMKAIS ICST DYQ+ECK
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  NTLSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDL 120
           NTL++VAHN SSN PK+Y KAA+LAT++EITKGYN SDSL+VEA NNA+IKMSVEDCKDL
Sbjct: 61  NTLNHVAHNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCKDL 120

Query: 121 LQFAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQ 180
           LQFAIDELQASYS VG+SDLHTDADR+ADIKNWLSAV+SYQQSCLDGL EFDPQLK++MQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DNLGVAAKLTSNALAIVSAVSNMLENYGLQLKVQPSGRKLLGTTEVGSDGFPTWLSGADR 240
           D L VA KLTSNALAIV+AVSN+L+NYGLQLKVQPSGR+LLGTTEV SDGFPTWL+GADR
Sbjct: 181 DGLDVAGKLTSNALAIVTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 KLLAARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVT 300
           KLLA++ GG +  PNAVVAKDGSGQYK+IAAALAAYPK L+GRYVIYVKAGIYDEYITVT
Sbjct: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300

Query: 301 KDMRNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQ 360
           KDM+NVFMYGDGPRKTIVTGRKSNRDG TTQNTASF+A+G+GFLCKSMGF+NTAGP+GHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTAQGRA+QKEITGLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGV+VIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540

Query: 541 QYTAGPFLLGRAWLPTTGGFYFLGLKN 565
           QYTAG FLLGR WLP TGG Y+LGLKN
Sbjct: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 567

BLAST of HG10009633 vs. NCBI nr
Match: XP_008466403.1 (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 995.7 bits (2573), Expect = 1.6e-286
Identity = 493/567 (86.95%), Postives = 533/567 (94.00%), Query Frame = 0

Query: 1   MVGKVVVSGLSLILVVGVALAVV---NKSSSSNAAAEDLSPKMKAISTICSTADYQQECK 60
           MVGKV+VS LSLILVVG+ALAVV   NK + SN   E++SPKMKAIS ICST DYQ+ECK
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  NTLSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDL 120
           NTL++VA N SSN PK+Y KAA+LAT++EITKGYN SDSL+VEA NNA+IKMSVEDC DL
Sbjct: 61  NTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDL 120

Query: 121 LQFAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQ 180
           LQFAIDELQASYS VG+SDLHTDADR+ADIKNWLSAV+SYQQSCLDGL EFDPQLK++MQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DNLGVAAKLTSNALAIVSAVSNMLENYGLQLKVQPSGRKLLGTTEVGSDGFPTWLSGADR 240
           D L VA KLTSNALAI++AVSN+L+NYGLQLKVQPSGR+LLGTTEV SDGFPTWL+GADR
Sbjct: 181 DGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 KLLAARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVT 300
           KLLA++ GG +  PNAVVAKDGSGQYK+IAAALAAYPK L+GRYVIYVKAGIYDEYITVT
Sbjct: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300

Query: 301 KDMRNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQ 360
           KDM+NVFMYGDGPRKTIVTGRKSNRDG TTQNTASF+A+G+GFLCKSMGF+NTAGP+GHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTAQGRA+QKEITGLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGV+VIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540

Query: 541 QYTAGPFLLGRAWLPTTGGFYFLGLKN 565
           QYTAG FLLGR WLP TGG Y+LGLKN
Sbjct: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 567

BLAST of HG10009633 vs. NCBI nr
Match: XP_004149499.1 (pectinesterase [Cucumis sativus] >KGN49780.1 hypothetical protein Csa_004678 [Cucumis sativus])

HSP 1 Score: 983.0 bits (2540), Expect = 1.1e-282
Identity = 485/567 (85.54%), Postives = 531/567 (93.65%), Query Frame = 0

Query: 1   MVGKVVVSGLSLILVVGVALAV---VNKSSSSNAAAEDLSPKMKAISTICSTADYQQECK 60
           M+GKV+VSGLSLILVVG+ALA+   +NKS++SN   ED+SPKMKA+S ICST DYQ ECK
Sbjct: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60

Query: 61  NTLSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDL 120
            TL +VA N SSN PK+Y +AA+LAT+ EITKGYN SDSL+VEA+ NA+IKMSVEDCKDL
Sbjct: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120

Query: 121 LQFAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQ 180
           LQFAIDELQASYSAVG+SDLHTD+DRVADIKNWLSAVISYQQSCLDGL EFDPQLK++MQ
Sbjct: 121 LQFAIDELQASYSAVGESDLHTDSDRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DNLGVAAKLTSNALAIVSAVSNMLENYGLQLKVQPSGRKLLGTTEVGSDGFPTWLSGADR 240
           D L VA KLTSNALAIV+AVSN+L+NY LQLKVQPSGR+LLGTT V  DGFPTWL+GADR
Sbjct: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240

Query: 241 KLLAARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVT 300
           KLLA++  G +  PNAVVAKDGSG+YK+IAAALAAYPK L+GRYVIYVKAGIYDEYIT+T
Sbjct: 241 KLLASKQRGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300

Query: 301 KDMRNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQ 360
           KDM+NVFMYGDGPRKTIVTGRKSNRDG TTQNTASF+A+G+GFLCKSMGF+NTAGPEGHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCV+SGTVDFIFGDSST+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTAQGRA+QKEITGLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSG+FALETCLYLEYGNRGPGANTNRRVRWKG +VIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540

Query: 541 QYTAGPFLLGRAWLPTTGGFYFLGLKN 565
           QYTAG FLLGR+WLPTTGG Y+LGLKN
Sbjct: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 567

BLAST of HG10009633 vs. NCBI nr
Match: XP_038875879.1 (LOW QUALITY PROTEIN: pectinesterase-like [Benincasa hispida])

HSP 1 Score: 972.6 bits (2513), Expect = 1.4e-279
Identity = 485/565 (85.84%), Postives = 525/565 (92.92%), Query Frame = 0

Query: 1   MVGKVVVSGLSLILVVGVALAVVNKSSSSNAAAEDL-SPKMKAISTICSTADYQQECKNT 60
           MVGKVVVSG+SLILVVGVALAVV   + +N +  DL SPKMKA+STICST DYQQEC+NT
Sbjct: 1   MVGKVVVSGISLILVVGVALAVVAMVTKNNNSDVDLSSPKMKAVSTICSTTDYQQECQNT 60

Query: 61  LSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDLLQ 120
           L+NVA NAS + PKEY KAAVLAT++EITKGYN +  LMVEA NNA+IKMSV+DCKDLLQ
Sbjct: 61  LTNVAQNASVDDPKEYAKAAVLATIQEITKGYNLTADLMVEAANNASIKMSVDDCKDLLQ 120

Query: 121 FAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQDN 180
            AIDEL ASYS VGD DLHT+ DRVADIKNWL+AVISYQQSCLDGLEEFDPQLK+KMQD 
Sbjct: 121 SAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQDG 180

Query: 181 LGVAAKLTSNALAIVSAVSNMLENYGLQLKVQPSGRKLLGTTEVGSDGFPTWLSGADRKL 240
           L VA KLTSNALAIV AVS++L+N+GLQLKVQPSGR+LL TTE+GSDGFPTW+SGADRKL
Sbjct: 181 LDVAGKLTSNALAIVGAVSDILKNFGLQLKVQPSGRRLLETTEIGSDGFPTWMSGADRKL 240

Query: 241 LAARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVTKD 300
           LA+RGGG + +PNAVVAKDGSGQYKSI AALAAYPK L+GRYVIYVKAGIYDEYITVTKD
Sbjct: 241 LASRGGGARVKPNAVVAKDGSGQYKSIGAALAAYPKALRGRYVIYVKAGIYDEYITVTKD 300

Query: 301 MRNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQAV 360
           M+NVFMYGDGPRKTIVTGRK+NRDGITTQNTASFSA+G+GFLCKSMGF NTAGPEGHQAV
Sbjct: 301 MKNVFMYGDGPRKTIVTGRKNNRDGITTQNTASFSAIGEGFLCKSMGFRNTAGPEGHQAV 360

Query: 361 ALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVR 420
           ALRVQSDRSAF+NCRMDG+QDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVR
Sbjct: 361 ALRVQSDRSAFYNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVR 420

Query: 421 RPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVI 480
           RPMDNQ+NTVTA GRA +KEI+GLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYARTVI
Sbjct: 421 RPMDNQKNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVI 480

Query: 481 MESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEALQY 540
           ME+TLGDFIQP GYMPWSGNFALETC Y EYGNRGPGANTNRRVRWKGVRVIGRNEALQY
Sbjct: 481 METTLGDFIQPDGYMPWSGNFALETCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEALQY 540

Query: 541 TAGPFLLGRAWLPTTGGFYFLGLKN 565
           TAGPFL+GRAWLP TGG Y LGLK+
Sbjct: 541 TAGPFLMGRAWLPATGGPYLLGLKH 565

BLAST of HG10009633 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 5.7e-122
Identity = 230/562 (40.93%), Postives = 347/562 (61.74%), Query Frame = 0

Query: 4   KVVVSGLSLILVVGVALAV---VNKSSSSNAAAEDLSPKMKAISTICSTADYQQECKNTL 63
           + V+  +S +L++ + +AV   V+ + S NA  E+++  +KAI  +C+  DY++ C++TL
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTL 74

Query: 64  SNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDLLQF 123
              A + S   P E VK A  AT+++I+     S + M+E   +   KM+++ CK+L+ +
Sbjct: 75  RKDAKDTSD--PLELVKTAFNATMKQISDVAKKSQT-MIELQKDPRAKMALDQCKELMDY 134

Query: 124 AIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQDNL 183
           AI EL  S+  +G  + H   + +  ++ WLSA IS++Q+CLDG +       E ++  L
Sbjct: 135 AIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKAL 194

Query: 184 GVAAKLTSNALAIVSAVSNMLENYGLQLKVQPSGRKLLGTTEVGSDGFPTWLSGADRKLL 243
             A +LT N LA+V+ +SN L   G     + + R+LL      S  FP+W+    R+LL
Sbjct: 195 KTAVQLTHNGLAMVTEMSNYL---GQMQIPEMNSRRLL------SQEFPSWMDARARRLL 254

Query: 244 AARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVTKDM 303
            A     + +P+ VVA+DGSGQYK+I  AL   PK     +V+++K GIY EY+ V + M
Sbjct: 255 NA--PMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSM 314

Query: 304 RNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQAVA 363
            ++   GDGP KT+++G KS +DGITT  TA+ + +GD F+ K++ F NTAG   HQAVA
Sbjct: 315 THLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVA 374

Query: 364 LRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVRR 423
           +RV +D S F+NC+ DG+QDTLY  +HRQFYR+C ISGT+DF+FGD++ + QN  ++VR+
Sbjct: 375 IRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRK 434

Query: 424 PMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIM 483
           P+ NQ   +TA GR   +E TG V+  C IV E    A + +  TYLGRPWK+Y+RT+IM
Sbjct: 435 PLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIM 494

Query: 484 ESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEALQYT 543
            + + DF+ P G+ PW G F L T  Y E  N GPGA   +RV W G++ +   E L++T
Sbjct: 495 NTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFT 554

Query: 544 AGPFLLGRAWLPTTGGFYFLGL 563
              ++ G AW+P  G  Y LGL
Sbjct: 555 PAQYIQGDAWIPGKGVPYILGL 562

BLAST of HG10009633 vs. ExPASy Swiss-Prot
Match: Q7Y201 (Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3702 GN=PME13 PE=2 SV=2)

HSP 1 Score: 439.1 bits (1128), Expect = 7.5e-122
Identity = 242/536 (45.15%), Postives = 343/536 (63.99%), Query Frame = 0

Query: 31  AAAEDLSPKM--KAISTICSTADYQQECKNTLSNVAHNA-SSNHPKEYVKAAVLATVEEI 90
           +AA+ + P    K I T+CS+  Y Q C+ TL N      + ++P  ++K+A+ A  E++
Sbjct: 95  SAAQSVKPGQGDKIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDL 154

Query: 91  TKGYNFSDSLMVEATNNATIKMSVEDCKDLLQFAIDELQASYSAVGDSDLHTDADRVADI 150
                  + ++   T N   K ++E CK L++ A +E  AS + +  +++++    V D+
Sbjct: 155 DL---VLEKVLSLKTENQDDKDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDL 214

Query: 151 KNWLSAVISYQQSCLDGLEEFDPQLKEKMQDNLGVAAKLTSNALAIVSAVSNMLENYGLQ 210
           ++WLSAV+SYQ++CLDG EE    LK +++ ++  +  LTSN+LA++   +   EN    
Sbjct: 215 ESWLSAVMSYQETCLDGFEE--GNLKSEVKTSVNSSQVLTSNSLALIKTFT---ENLSPV 274

Query: 211 LKVQPSGRKLLGTTEVGSDGFPTWLSGADRKLLAARGGGGQARPNAVVAKDGSGQYKSIA 270
           +KV    R LL       D  P+W+S  DR++L A       +PNA VAKDGSG + +I 
Sbjct: 275 MKVVE--RHLL-------DDIPSWVSNDDRRMLRAVDVKA-LKPNATVAKDGSGDFTTIN 334

Query: 271 AALAAYPKTLKGRYVIYVKAGIYDEYITVTKDMRNVFMYGDGPRKTIVTGRKSNRDGITT 330
            AL A P+  +GRY+IYVK GIYDEY+TV K   N+ M GDG +KTIVTG KS+   I T
Sbjct: 335 DALRAMPEKYEGRYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRT 394

Query: 331 QNTASFSALGDGFLCKSMGFSNTAGPEGHQAVALRVQSDRSAFFNCRMDGHQDTLYVQTH 390
             TA+F A G+GF+ +SMGF NTAGPEGHQAVA+RVQSDRS F NCR +G+QDTLY  TH
Sbjct: 395 FLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTH 454

Query: 391 RQFYRNCVISGTVDFIFGDSSTIIQNSLIIVRRPMDNQQNTVTAQGRAQQKEITGLVIHN 450
           RQ+YR+CVI GT+DFIFGD++ I QN  I +R+ +  Q+NTVTAQGR  + + TG V+HN
Sbjct: 455 RQYYRSCVIVGTIDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHN 514

Query: 451 CRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMESTLGDFIQPAGYMPW-SGNFALETCL 510
           C+I   + L   + +  +YLGRPWK Y+RT+IMES + + I P G++ W   +FA++T  
Sbjct: 515 CKIAANEDLKPVKEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLY 574

Query: 511 YLEYGNRGPGANTNRRVRWKGVRVIGRNEALQYTAGPFLLGRAWLPTTGGFYFLGL 563
           Y EY N+G   +T  RV+W G +VI + EAL YT GPFL G  W+  +G    LGL
Sbjct: 575 YAEYNNKGSSGDTTSRVKWPGFKVINKEEALNYTVGPFLQG-DWISASGSPVKLGL 611

BLAST of HG10009633 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 438.3 bits (1126), Expect = 1.3e-121
Identity = 222/565 (39.29%), Postives = 349/565 (61.77%), Query Frame = 0

Query: 4   KVVVSGLSLILVVGVALAV-----VNKSSSSNAAAEDLSPKMKAISTICSTADYQQECKN 63
           + +V  +S +L++ + +AV     +NK    +    +++  +KA+  +C+  DY++ C++
Sbjct: 14  RYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCED 73

Query: 64  TLSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDLL 123
           TL  + +  ++  P E VK A   T+++IT     S ++M E   ++  +M+++ CK+L+
Sbjct: 74  TL--IKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIM-ELQKDSRTRMALDQCKELM 133

Query: 124 QFAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQD 183
            +A+DEL  S+  +G  + H   + + +++ WLSA IS++++CL+G +       E M+ 
Sbjct: 134 DYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKK 193

Query: 184 NLGVAAKLTSNALAIVSAVSNMLENYGLQLKVQP-SGRKLLGTTEVGSDGFPTWLSGADR 243
            L  A +LT N LAI+S +SN +     Q+++   + R+LL      ++GFP+W+    R
Sbjct: 194 ALKTAIELTHNGLAIISEMSNFVG----QMQIPGLNSRRLL------AEGFPSWVDQRGR 253

Query: 244 KLLAARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVT 303
           KLL A       +P+ VVA+DGSGQYK+I  AL   PK     +V+++KAG+Y EY+ V 
Sbjct: 254 KLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVN 313

Query: 304 KDMRNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQ 363
           K M ++   GDGP KTI++G K+ +DGITT  TA+ + +G+ F+ K++GF NTAG   HQ
Sbjct: 314 KTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQ 373

Query: 364 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 423
           AVA+RVQSD S FFNCR DG+QDTLY  +HRQF+R+C ISGT+DF+FGD++ + QN  ++
Sbjct: 374 AVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLL 433

Query: 424 VRRPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 483
           VR+P+ NQ   +TA GR   +E TG V   C I  E    A +     YLGRPWK+Y+RT
Sbjct: 434 VRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRT 493

Query: 484 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEAL 543
           +IM + + DF+QP G+ PW G+F L+T  Y E  N GPG+    RV W G++ +   + L
Sbjct: 494 IIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDIL 553

Query: 544 QYTAGPFLLGRAWLPTTGGFYFLGL 563
           ++T   ++ G  W+P  G  Y  GL
Sbjct: 554 KFTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of HG10009633 vs. ExPASy Swiss-Prot
Match: Q9SMY6 (Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3702 GN=PME45 PE=2 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 1.3e-121
Identity = 245/560 (43.75%), Postives = 343/560 (61.25%), Query Frame = 0

Query: 24  NKSSSSNAAAEDLSPKM--------------KAISTICSTADYQQECKNTLSN-VAHNAS 83
           NKS  S   +E  SPK               K I T+C++  Y+  C+NTL N    +  
Sbjct: 62  NKSKDSPTKSESPSPKPPSSAAQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTP 121

Query: 84  SNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDLLQFAIDELQAS 143
              P+  +K+A++A  +++ + +    SL    T N   K ++  CK L+  A +EL  S
Sbjct: 122 QTDPRSLLKSAIVAVNDDLDQVFKRVLSL---KTENKDDKDAIAQCKLLVDEAKEELGTS 181

Query: 144 YSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQDNLGVAAKLTS 203
              + DS+++  A  V D+ +WLSAV+SYQ++C+DG EE   +LK +++ N   +  LTS
Sbjct: 182 MKRINDSEVNNFAKIVPDLDSWLSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTS 241

Query: 204 NALAIVSAVSNMLENY-----GLQLKVQPSGRKLLGTTEVGSDGFPTWLSGADRKLLAAR 263
           N+LA++ ++   L +       L L+ + S ++        +D   +WLS  +R++L A 
Sbjct: 242 NSLAMIKSLDGYLSSVPKVKTRLLLEARSSAKE--------TDHITSWLSNKERRMLKAV 301

Query: 264 GGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVTKDMRNV 323
                 +PNA VAKDGSG + +I AAL A P   +GRY IY+K GIYDE + + K   NV
Sbjct: 302 DVKA-LKPNATVAKDGSGNFTTINAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNV 361

Query: 324 FMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQAVALRV 383
            M GDG +KTIVTG KS+   I T  TA+F A G+GF+ +SMGF NTAGPEGHQAVA+RV
Sbjct: 362 TMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRV 421

Query: 384 QSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVRRPMD 443
           QSDRS F NCR +G+QDTLY  THRQ+YR+CVI GTVDFIFGD++ I QN  I +R+ + 
Sbjct: 422 QSDRSVFLNCRFEGYQDTLYAYTHRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLP 481

Query: 444 NQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMEST 503
            Q+NTVTAQGR  + + TG VIHNC + P + L   + +  +YLGRPWK ++RTV+MEST
Sbjct: 482 GQKNTVTAQGRVDKFQTTGFVIHNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMEST 541

Query: 504 LGDFIQPAGYMPW-SGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEALQYTAG 563
           + D I P G++ W   +FA++T  Y EY N GP   T  RV+W G RV+ + EA+++T G
Sbjct: 542 IEDVIDPVGWLRWQETDFAIDTLSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVG 601

BLAST of HG10009633 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 1.1e-117
Identity = 227/553 (41.05%), Postives = 333/553 (60.22%), Query Frame = 0

Query: 6   VVSGLSLILVVGVALAVV-NKSSSSNAAAEDLSPKMKAISTICSTADYQQECKNTLSNVA 65
           V++ L +++VV V +    N S S       +     A+  +C+  DY++ C N+L    
Sbjct: 17  VITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDYKETCVNSLMKA- 76

Query: 66  HNASSNHPKEYVKAAVLATVEEITKGY-NFSDSLMVEATNNATIKMSVEDCKDLLQFAID 125
            +  S  P + +K     T+  I       S  L  +A N+   K ++E C+ L+  A D
Sbjct: 77  -SPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALELCEKLMNDATD 136

Query: 126 ELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQDNLGVA 185
           +L+          +    D V D++ WLS  I+YQQ+C+D  EE + +L + MQ     +
Sbjct: 137 DLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLSQDMQKIFKTS 196

Query: 186 AKLTSNALAIVSAVSNMLENY---GLQLKVQPSGRKLLGTTEVGSDGFPTWLSGADRKLL 245
            +LTSN LA+++ +SN+L  +   G+   +    RKLL       DG P+W+    R+L+
Sbjct: 197 RELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLS----AEDGIPSWVGPNTRRLM 256

Query: 246 AARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVTKDM 305
           A +GG    + N VVA DGSGQYK+I  AL A PK  +  +VIY+K G+Y+E + VTK M
Sbjct: 257 ATKGG---VKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEKVDVTKKM 316

Query: 306 RNVFMYGDGPRKTIVTGRKSNRDG-ITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQAV 365
            +V   GDGP KT +TG  +   G + T  TA+ +  GD F  K++GF NTAGPEGHQAV
Sbjct: 317 THVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAGPEGHQAV 376

Query: 366 ALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVR 425
           ALRV +D + F+NC++DG+QDTLYV +HRQF+R+C +SGTVDFIFGD   ++QN  I+VR
Sbjct: 377 ALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQNCNIVVR 436

Query: 426 RPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVI 485
           +PM +Q   +TAQGR+ ++E TGLV+ NC I  E      +     YLGRPWK+++RT+I
Sbjct: 437 KPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWKEFSRTII 496

Query: 486 MESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEALQY 545
           M +T+ D I PAG++PW+G+FAL T  Y EY N GPG+N  +RV+W G++ +   +AL++
Sbjct: 497 MGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSPKQALRF 556

Query: 546 TAGPFLLGRAWLP 553
           T   FL G  W+P
Sbjct: 557 TPARFLRGNLWIP 560

BLAST of HG10009633 vs. ExPASy TrEMBL
Match: A0A5A7U4V7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00110 PE=3 SV=1)

HSP 1 Score: 1001.9 bits (2589), Expect = 1.1e-288
Identity = 496/567 (87.48%), Postives = 535/567 (94.36%), Query Frame = 0

Query: 1   MVGKVVVSGLSLILVVGVALAVV---NKSSSSNAAAEDLSPKMKAISTICSTADYQQECK 60
           MVGKV+VS LSLILVVG+ALAVV   NK + SN   E++SPKMKAIS ICST DYQ+ECK
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  NTLSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDL 120
           NTL++VAHN SSN PK+Y KAA+LAT++EITKGYN SDSL+VEA NNA+IKMSVEDCKDL
Sbjct: 61  NTLNHVAHNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCKDL 120

Query: 121 LQFAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQ 180
           LQFAIDELQASYS VG+SDLHTDADR+ADIKNWLSAV+SYQQSCLDGL EFDPQLK++MQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DNLGVAAKLTSNALAIVSAVSNMLENYGLQLKVQPSGRKLLGTTEVGSDGFPTWLSGADR 240
           D L VA KLTSNALAIV+AVSN+L+NYGLQLKVQPSGR+LLGTTEV SDGFPTWL+GADR
Sbjct: 181 DGLDVAGKLTSNALAIVTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 KLLAARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVT 300
           KLLA++ GG +  PNAVVAKDGSGQYK+IAAALAAYPK L+GRYVIYVKAGIYDEYITVT
Sbjct: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300

Query: 301 KDMRNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQ 360
           KDM+NVFMYGDGPRKTIVTGRKSNRDG TTQNTASF+A+G+GFLCKSMGF+NTAGP+GHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTAQGRA+QKEITGLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGV+VIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540

Query: 541 QYTAGPFLLGRAWLPTTGGFYFLGLKN 565
           QYTAG FLLGR WLP TGG Y+LGLKN
Sbjct: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 567

BLAST of HG10009633 vs. ExPASy TrEMBL
Match: A0A1S3CSH2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503817 PE=3 SV=1)

HSP 1 Score: 995.7 bits (2573), Expect = 7.7e-287
Identity = 493/567 (86.95%), Postives = 533/567 (94.00%), Query Frame = 0

Query: 1   MVGKVVVSGLSLILVVGVALAVV---NKSSSSNAAAEDLSPKMKAISTICSTADYQQECK 60
           MVGKV+VS LSLILVVG+ALAVV   NK + SN   E++SPKMKAIS ICST DYQ+ECK
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  NTLSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDL 120
           NTL++VA N SSN PK+Y KAA+LAT++EITKGYN SDSL+VEA NNA+IKMSVEDC DL
Sbjct: 61  NTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDL 120

Query: 121 LQFAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQ 180
           LQFAIDELQASYS VG+SDLHTDADR+ADIKNWLSAV+SYQQSCLDGL EFDPQLK++MQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DNLGVAAKLTSNALAIVSAVSNMLENYGLQLKVQPSGRKLLGTTEVGSDGFPTWLSGADR 240
           D L VA KLTSNALAI++AVSN+L+NYGLQLKVQPSGR+LLGTTEV SDGFPTWL+GADR
Sbjct: 181 DGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 KLLAARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVT 300
           KLLA++ GG +  PNAVVAKDGSGQYK+IAAALAAYPK L+GRYVIYVKAGIYDEYITVT
Sbjct: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300

Query: 301 KDMRNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQ 360
           KDM+NVFMYGDGPRKTIVTGRKSNRDG TTQNTASF+A+G+GFLCKSMGF+NTAGP+GHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTAQGRA+QKEITGLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGV+VIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540

Query: 541 QYTAGPFLLGRAWLPTTGGFYFLGLKN 565
           QYTAG FLLGR WLP TGG Y+LGLKN
Sbjct: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 567

BLAST of HG10009633 vs. ExPASy TrEMBL
Match: A0A0A0KJ73 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G125700 PE=3 SV=1)

HSP 1 Score: 983.0 bits (2540), Expect = 5.1e-283
Identity = 485/567 (85.54%), Postives = 531/567 (93.65%), Query Frame = 0

Query: 1   MVGKVVVSGLSLILVVGVALAV---VNKSSSSNAAAEDLSPKMKAISTICSTADYQQECK 60
           M+GKV+VSGLSLILVVG+ALA+   +NKS++SN   ED+SPKMKA+S ICST DYQ ECK
Sbjct: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60

Query: 61  NTLSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDL 120
            TL +VA N SSN PK+Y +AA+LAT+ EITKGYN SDSL+VEA+ NA+IKMSVEDCKDL
Sbjct: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120

Query: 121 LQFAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQ 180
           LQFAIDELQASYSAVG+SDLHTD+DRVADIKNWLSAVISYQQSCLDGL EFDPQLK++MQ
Sbjct: 121 LQFAIDELQASYSAVGESDLHTDSDRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DNLGVAAKLTSNALAIVSAVSNMLENYGLQLKVQPSGRKLLGTTEVGSDGFPTWLSGADR 240
           D L VA KLTSNALAIV+AVSN+L+NY LQLKVQPSGR+LLGTT V  DGFPTWL+GADR
Sbjct: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240

Query: 241 KLLAARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVT 300
           KLLA++  G +  PNAVVAKDGSG+YK+IAAALAAYPK L+GRYVIYVKAGIYDEYIT+T
Sbjct: 241 KLLASKQRGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300

Query: 301 KDMRNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQ 360
           KDM+NVFMYGDGPRKTIVTGRKSNRDG TTQNTASF+A+G+GFLCKSMGF+NTAGPEGHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCV+SGTVDFIFGDSST+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTAQGRA+QKEITGLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSG+FALETCLYLEYGNRGPGANTNRRVRWKG +VIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540

Query: 541 QYTAGPFLLGRAWLPTTGGFYFLGLKN 565
           QYTAG FLLGR+WLPTTGG Y+LGLKN
Sbjct: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 567

BLAST of HG10009633 vs. ExPASy TrEMBL
Match: A0A5A7U9J8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00100 PE=3 SV=1)

HSP 1 Score: 946.8 bits (2446), Expect = 4.1e-272
Identity = 462/567 (81.48%), Postives = 521/567 (91.89%), Query Frame = 0

Query: 1   MVGKVVVSGLSLILVVGVALAV---VNKSSSSNAAAEDLSPKMKAISTICSTADYQQECK 60
           MV KV++ G+SLILVVGVALAV   V+ + SS+AA EDLSPKMKA+STIC+ A+YQQEC+
Sbjct: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDL 120
           NTLSN AHNASS+ PKEYV+AA+L+T+EE+ KGYN +D  M+EA NN +IKM VEDC+DL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQ 180
           LQFAID+LQASYS VG+ DLHT+ADRVADIKNWL++VISYQQSCLDGLEEFDPQLK+KMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DNLGVAAKLTSNALAIVSAVSNMLENYGLQLKVQPSGRKLLGTTEVGSDGFPTWLSGADR 240
           D L  A KLTSNALAIV AVS++L ++GLQLK QPSGR+LLGTTEVG DG+PTWL+GADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLAARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVT 300
           KLLA+RGGG + +PNAVVAKDGSGQ+K+I+AALAAYPK L+GRYVIYVKAGIY+EY+ +T
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMRNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQ 360
           KDM+N+FMYGDGP+KTIVTG KSNR G TTQ+TA+F A+G+GFLCKSMGF NTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDG+QDTLYVQT RQFYRNCVISGTVDFIFGDS+TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTA GRA  KE++GLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEAL 540
           VIMESTLGDFIQP GYMPWSGNFALETCLYLEYGNRGPGA TNRRVRWKGV+VIGRNEA+
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QYTAGPFLLGRAWLPTTGGFYFLGLKN 565
           Q+TAGPFL GRAWLPTTGG Y LGLK+
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of HG10009633 vs. ExPASy TrEMBL
Match: A0A1S3CR27 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC107990290 PE=3 SV=1)

HSP 1 Score: 945.7 bits (2443), Expect = 9.1e-272
Identity = 461/567 (81.31%), Postives = 521/567 (91.89%), Query Frame = 0

Query: 1   MVGKVVVSGLSLILVVGVALAV---VNKSSSSNAAAEDLSPKMKAISTICSTADYQQECK 60
           MV KV++ G++LILVVGVALAV   V+ + SS+AA EDLSPKMKA+STIC+ A+YQQEC+
Sbjct: 1   MVNKVILPGITLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDL 120
           NTLSN AHNASS+ PKEYV+AA+L+T+EE+ KGYN +D  M+EA NN +IKM VEDC+DL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQ 180
           LQFAID+LQASYS VG+ DLHT+ADRVADIKNWL++VISYQQSCLDGLEEFDPQLK+KMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DNLGVAAKLTSNALAIVSAVSNMLENYGLQLKVQPSGRKLLGTTEVGSDGFPTWLSGADR 240
           D L  A KLTSNALAIV AVS++L ++GLQLK QPSGR+LLGTTEVG DG+PTWL+GADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLAARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVT 300
           KLLA+RGGG + +PNAVVAKDGSGQ+K+I+AALAAYPK L+GRYVIYVKAGIY+EY+ +T
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMRNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQ 360
           KDM+N+FMYGDGP+KTIVTG KSNR G TTQ+TA+F A+G+GFLCKSMGF NTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDG+QDTLYVQT RQFYRNCVISGTVDFIFGDS+TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTA GRA  KE++GLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEAL 540
           VIMESTLGDFIQP GYMPWSGNFALETCLYLEYGNRGPGA TNRRVRWKGV+VIGRNEA+
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QYTAGPFLLGRAWLPTTGGFYFLGLKN 565
           Q+TAGPFL GRAWLPTTGG Y LGLK+
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of HG10009633 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 439.5 bits (1129), Expect = 4.1e-123
Identity = 230/562 (40.93%), Postives = 347/562 (61.74%), Query Frame = 0

Query: 4   KVVVSGLSLILVVGVALAV---VNKSSSSNAAAEDLSPKMKAISTICSTADYQQECKNTL 63
           + V+  +S +L++ + +AV   V+ + S NA  E+++  +KAI  +C+  DY++ C++TL
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTL 74

Query: 64  SNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDLLQF 123
              A + S   P E VK A  AT+++I+     S + M+E   +   KM+++ CK+L+ +
Sbjct: 75  RKDAKDTSD--PLELVKTAFNATMKQISDVAKKSQT-MIELQKDPRAKMALDQCKELMDY 134

Query: 124 AIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQDNL 183
           AI EL  S+  +G  + H   + +  ++ WLSA IS++Q+CLDG +       E ++  L
Sbjct: 135 AIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKAL 194

Query: 184 GVAAKLTSNALAIVSAVSNMLENYGLQLKVQPSGRKLLGTTEVGSDGFPTWLSGADRKLL 243
             A +LT N LA+V+ +SN L   G     + + R+LL      S  FP+W+    R+LL
Sbjct: 195 KTAVQLTHNGLAMVTEMSNYL---GQMQIPEMNSRRLL------SQEFPSWMDARARRLL 254

Query: 244 AARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVTKDM 303
            A     + +P+ VVA+DGSGQYK+I  AL   PK     +V+++K GIY EY+ V + M
Sbjct: 255 NA--PMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSM 314

Query: 304 RNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQAVA 363
            ++   GDGP KT+++G KS +DGITT  TA+ + +GD F+ K++ F NTAG   HQAVA
Sbjct: 315 THLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVA 374

Query: 364 LRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVRR 423
           +RV +D S F+NC+ DG+QDTLY  +HRQFYR+C ISGT+DF+FGD++ + QN  ++VR+
Sbjct: 375 IRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRK 434

Query: 424 PMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIM 483
           P+ NQ   +TA GR   +E TG V+  C IV E    A + +  TYLGRPWK+Y+RT+IM
Sbjct: 435 PLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIM 494

Query: 484 ESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEALQYT 543
            + + DF+ P G+ PW G F L T  Y E  N GPGA   +RV W G++ +   E L++T
Sbjct: 495 NTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFT 554

Query: 544 AGPFLLGRAWLPTTGGFYFLGL 563
              ++ G AW+P  G  Y LGL
Sbjct: 555 PAQYIQGDAWIPGKGVPYILGL 562

BLAST of HG10009633 vs. TAIR 10
Match: AT2G26450.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 439.1 bits (1128), Expect = 5.3e-123
Identity = 242/536 (45.15%), Postives = 343/536 (63.99%), Query Frame = 0

Query: 31  AAAEDLSPKM--KAISTICSTADYQQECKNTLSNVAHNA-SSNHPKEYVKAAVLATVEEI 90
           +AA+ + P    K I T+CS+  Y Q C+ TL N      + ++P  ++K+A+ A  E++
Sbjct: 95  SAAQSVKPGQGDKIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDL 154

Query: 91  TKGYNFSDSLMVEATNNATIKMSVEDCKDLLQFAIDELQASYSAVGDSDLHTDADRVADI 150
                  + ++   T N   K ++E CK L++ A +E  AS + +  +++++    V D+
Sbjct: 155 DL---VLEKVLSLKTENQDDKDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDL 214

Query: 151 KNWLSAVISYQQSCLDGLEEFDPQLKEKMQDNLGVAAKLTSNALAIVSAVSNMLENYGLQ 210
           ++WLSAV+SYQ++CLDG EE    LK +++ ++  +  LTSN+LA++   +   EN    
Sbjct: 215 ESWLSAVMSYQETCLDGFEE--GNLKSEVKTSVNSSQVLTSNSLALIKTFT---ENLSPV 274

Query: 211 LKVQPSGRKLLGTTEVGSDGFPTWLSGADRKLLAARGGGGQARPNAVVAKDGSGQYKSIA 270
           +KV    R LL       D  P+W+S  DR++L A       +PNA VAKDGSG + +I 
Sbjct: 275 MKVVE--RHLL-------DDIPSWVSNDDRRMLRAVDVKA-LKPNATVAKDGSGDFTTIN 334

Query: 271 AALAAYPKTLKGRYVIYVKAGIYDEYITVTKDMRNVFMYGDGPRKTIVTGRKSNRDGITT 330
            AL A P+  +GRY+IYVK GIYDEY+TV K   N+ M GDG +KTIVTG KS+   I T
Sbjct: 335 DALRAMPEKYEGRYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRT 394

Query: 331 QNTASFSALGDGFLCKSMGFSNTAGPEGHQAVALRVQSDRSAFFNCRMDGHQDTLYVQTH 390
             TA+F A G+GF+ +SMGF NTAGPEGHQAVA+RVQSDRS F NCR +G+QDTLY  TH
Sbjct: 395 FLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTH 454

Query: 391 RQFYRNCVISGTVDFIFGDSSTIIQNSLIIVRRPMDNQQNTVTAQGRAQQKEITGLVIHN 450
           RQ+YR+CVI GT+DFIFGD++ I QN  I +R+ +  Q+NTVTAQGR  + + TG V+HN
Sbjct: 455 RQYYRSCVIVGTIDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHN 514

Query: 451 CRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMESTLGDFIQPAGYMPW-SGNFALETCL 510
           C+I   + L   + +  +YLGRPWK Y+RT+IMES + + I P G++ W   +FA++T  
Sbjct: 515 CKIAANEDLKPVKEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLY 574

Query: 511 YLEYGNRGPGANTNRRVRWKGVRVIGRNEALQYTAGPFLLGRAWLPTTGGFYFLGL 563
           Y EY N+G   +T  RV+W G +VI + EAL YT GPFL G  W+  +G    LGL
Sbjct: 575 YAEYNNKGSSGDTTSRVKWPGFKVINKEEALNYTVGPFLQG-DWISASGSPVKLGL 611

BLAST of HG10009633 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 438.3 bits (1126), Expect = 9.1e-123
Identity = 222/565 (39.29%), Postives = 349/565 (61.77%), Query Frame = 0

Query: 4   KVVVSGLSLILVVGVALAV-----VNKSSSSNAAAEDLSPKMKAISTICSTADYQQECKN 63
           + +V  +S +L++ + +AV     +NK    +    +++  +KA+  +C+  DY++ C++
Sbjct: 14  RYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCED 73

Query: 64  TLSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDLL 123
           TL  + +  ++  P E VK A   T+++IT     S ++M E   ++  +M+++ CK+L+
Sbjct: 74  TL--IKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIM-ELQKDSRTRMALDQCKELM 133

Query: 124 QFAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQD 183
            +A+DEL  S+  +G  + H   + + +++ WLSA IS++++CL+G +       E M+ 
Sbjct: 134 DYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKK 193

Query: 184 NLGVAAKLTSNALAIVSAVSNMLENYGLQLKVQP-SGRKLLGTTEVGSDGFPTWLSGADR 243
            L  A +LT N LAI+S +SN +     Q+++   + R+LL      ++GFP+W+    R
Sbjct: 194 ALKTAIELTHNGLAIISEMSNFVG----QMQIPGLNSRRLL------AEGFPSWVDQRGR 253

Query: 244 KLLAARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVT 303
           KLL A       +P+ VVA+DGSGQYK+I  AL   PK     +V+++KAG+Y EY+ V 
Sbjct: 254 KLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVN 313

Query: 304 KDMRNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQ 363
           K M ++   GDGP KTI++G K+ +DGITT  TA+ + +G+ F+ K++GF NTAG   HQ
Sbjct: 314 KTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQ 373

Query: 364 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 423
           AVA+RVQSD S FFNCR DG+QDTLY  +HRQF+R+C ISGT+DF+FGD++ + QN  ++
Sbjct: 374 AVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLL 433

Query: 424 VRRPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 483
           VR+P+ NQ   +TA GR   +E TG V   C I  E    A +     YLGRPWK+Y+RT
Sbjct: 434 VRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRT 493

Query: 484 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEAL 543
           +IM + + DF+QP G+ PW G+F L+T  Y E  N GPG+    RV W G++ +   + L
Sbjct: 494 IIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDIL 553

Query: 544 QYTAGPFLLGRAWLPTTGGFYFLGL 563
           ++T   ++ G  W+P  G  Y  GL
Sbjct: 554 KFTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of HG10009633 vs. TAIR 10
Match: AT4G33230.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 438.3 bits (1126), Expect = 9.1e-123
Identity = 245/560 (43.75%), Postives = 343/560 (61.25%), Query Frame = 0

Query: 24  NKSSSSNAAAEDLSPKM--------------KAISTICSTADYQQECKNTLSN-VAHNAS 83
           NKS  S   +E  SPK               K I T+C++  Y+  C+NTL N    +  
Sbjct: 62  NKSKDSPTKSESPSPKPPSSAAQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTP 121

Query: 84  SNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDLLQFAIDELQAS 143
              P+  +K+A++A  +++ + +    SL    T N   K ++  CK L+  A +EL  S
Sbjct: 122 QTDPRSLLKSAIVAVNDDLDQVFKRVLSL---KTENKDDKDAIAQCKLLVDEAKEELGTS 181

Query: 144 YSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQDNLGVAAKLTS 203
              + DS+++  A  V D+ +WLSAV+SYQ++C+DG EE   +LK +++ N   +  LTS
Sbjct: 182 MKRINDSEVNNFAKIVPDLDSWLSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTS 241

Query: 204 NALAIVSAVSNMLENY-----GLQLKVQPSGRKLLGTTEVGSDGFPTWLSGADRKLLAAR 263
           N+LA++ ++   L +       L L+ + S ++        +D   +WLS  +R++L A 
Sbjct: 242 NSLAMIKSLDGYLSSVPKVKTRLLLEARSSAKE--------TDHITSWLSNKERRMLKAV 301

Query: 264 GGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVTKDMRNV 323
                 +PNA VAKDGSG + +I AAL A P   +GRY IY+K GIYDE + + K   NV
Sbjct: 302 DVKA-LKPNATVAKDGSGNFTTINAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNV 361

Query: 324 FMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQAVALRV 383
            M GDG +KTIVTG KS+   I T  TA+F A G+GF+ +SMGF NTAGPEGHQAVA+RV
Sbjct: 362 TMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRV 421

Query: 384 QSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVRRPMD 443
           QSDRS F NCR +G+QDTLY  THRQ+YR+CVI GTVDFIFGD++ I QN  I +R+ + 
Sbjct: 422 QSDRSVFLNCRFEGYQDTLYAYTHRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLP 481

Query: 444 NQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMEST 503
            Q+NTVTAQGR  + + TG VIHNC + P + L   + +  +YLGRPWK ++RTV+MEST
Sbjct: 482 GQKNTVTAQGRVDKFQTTGFVIHNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMEST 541

Query: 504 LGDFIQPAGYMPW-SGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEALQYTAG 563
           + D I P G++ W   +FA++T  Y EY N GP   T  RV+W G RV+ + EA+++T G
Sbjct: 542 IEDVIDPVGWLRWQETDFAIDTLSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVG 601

BLAST of HG10009633 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 425.2 bits (1092), Expect = 7.9e-119
Identity = 227/553 (41.05%), Postives = 333/553 (60.22%), Query Frame = 0

Query: 6   VVSGLSLILVVGVALAVV-NKSSSSNAAAEDLSPKMKAISTICSTADYQQECKNTLSNVA 65
           V++ L +++VV V +    N S S       +     A+  +C+  DY++ C N+L    
Sbjct: 17  VITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDYKETCVNSLMKA- 76

Query: 66  HNASSNHPKEYVKAAVLATVEEITKGY-NFSDSLMVEATNNATIKMSVEDCKDLLQFAID 125
            +  S  P + +K     T+  I       S  L  +A N+   K ++E C+ L+  A D
Sbjct: 77  -SPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALELCEKLMNDATD 136

Query: 126 ELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQDNLGVA 185
           +L+          +    D V D++ WLS  I+YQQ+C+D  EE + +L + MQ     +
Sbjct: 137 DLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLSQDMQKIFKTS 196

Query: 186 AKLTSNALAIVSAVSNMLENY---GLQLKVQPSGRKLLGTTEVGSDGFPTWLSGADRKLL 245
            +LTSN LA+++ +SN+L  +   G+   +    RKLL       DG P+W+    R+L+
Sbjct: 197 RELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLS----AEDGIPSWVGPNTRRLM 256

Query: 246 AARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVTKDM 305
           A +GG    + N VVA DGSGQYK+I  AL A PK  +  +VIY+K G+Y+E + VTK M
Sbjct: 257 ATKGG---VKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEKVDVTKKM 316

Query: 306 RNVFMYGDGPRKTIVTGRKSNRDG-ITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQAV 365
            +V   GDGP KT +TG  +   G + T  TA+ +  GD F  K++GF NTAGPEGHQAV
Sbjct: 317 THVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAGPEGHQAV 376

Query: 366 ALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVR 425
           ALRV +D + F+NC++DG+QDTLYV +HRQF+R+C +SGTVDFIFGD   ++QN  I+VR
Sbjct: 377 ALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQNCNIVVR 436

Query: 426 RPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVI 485
           +PM +Q   +TAQGR+ ++E TGLV+ NC I  E      +     YLGRPWK+++RT+I
Sbjct: 437 KPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWKEFSRTII 496

Query: 486 MESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEALQY 545
           M +T+ D I PAG++PW+G+FAL T  Y EY N GPG+N  +RV+W G++ +   +AL++
Sbjct: 497 MGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSPKQALRF 556

Query: 546 TAGPFLLGRAWLP 553
           T   FL G  W+P
Sbjct: 557 TPARFLRGNLWIP 560