HG10009588 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10009588
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Cytochrome b561 domain-containing protein
Location	Chr06: 7812187 .. 7813551 (-)
RNA-Seq Expression	HG10009588
Synteny	HG10009588

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACAGGGCAAGTTTCAGGCACACTGCTTCTGCAATTACTGTCTTTGCACATATTTTTGGTCTCACTGCCTGCATTCTTATGCTTGTTTGGTTGCTGCATTATCGTGGCGGCCTCGCCTACGATTCTCCCAATCCCGACCTTGTTTTCAATGTAAGGATTCGCTTGTTTCTTCGTCATTTTCCTTCCCTTGTGGCCTTTGATATCACTCGTGTTTGATATACAATATCAATCATGTCAACTCGAATTTATTATTAACTAAAAAATATATATTTTACTTTGTGAGGTATAGTATACTTATATTTATTTTAATCCTATTTCAATCAATAATTTCTTTTTCTCTCTTCTACCAACTTCTCTATAAAGTAATTTATCAAGTTTTTTGGAATATAATTTTCAATGATAAAGGAAAAAAATGATATTGAGTTACTAAATGTCTCATAAATTGGAGTTTCTTCATTATTAACGACTTGCAAATCTTTTATAATTGGAGTTAAGATTGTTACTAGTAAGGGTCCTTTGAATTGGATAAAGCTTAAAATAAAATAACTAGGTTAGATCCAGAGTCTCTTCTTCTGACTAATATAACCAACATAGAAGTGTTAATATGATAGGAAAGGAACTAACGTCGCAGGAGTTTTGTTTTTGGCAGTGCCATCCATTCTTCATGCTCCTTGGTTTCGTGTTCATGTCGGGGGAAGGTACTACTGCTAGCTCGAGTTCATTCAACTAATATTTTAAAGATTTAATATGAAAAAAGAAAATAAAAAATAACCATGATTGAATTGGGTGTGAGCAGCAATGATGGCATACAAGACAGTAAGGGCAGAAAAGAAAACAAAGAAGATAGTTCACATGGCTCTGCATTTTGTAGCCATTTGCATGGGAATTGCAGGGATAAACGCTGCTTTCAAGTTTCATGATCAGGTGAACTTGGAAGATATGTTTAGTTTACATTCATGGATTGGCATTGTCACTTTCTGCCTTTATGGCTTACAGGTATAACAAGTTTTAATTATGCCCTTAAACTTTGAAACTCCGATCATTTTTTATAAACCGTACACTGACTTACAATGGTTTGGGCAGTGGCTGTTGGGACTATGTACATTCTTGTTTCCAGGAGCAGATGCAGCGACTAAGGCCAGACTGCTGCCATGGCACATAAGTGGAGGAAGAGCTTTGCTGCTGATGATAATATGCACAGCCTTGACTGGCTTGTCTGAGAAGGCTACTTCTTTGAGACTGGCTGGCAACCATGAGTCTCGTTTGCTTAACTTCACTGCTTTCTTTGTCCTCCTCTTTGGCATTTCCATTGACCTCTCCATCGGTTTTGCTCGGTTTATGCACGCTGATCTCACCGTTTAG

mRNA sequence

ATGGACAGGGCAAGTTTCAGGCACACTGCTTCTGCAATTACTGTCTTTGCACATATTTTTGGTCTCACTGCCTGCATTCTTATGCTTGTTTGGTTGCTGCATTATCGTGGCGGCCTCGCCTACGATTCTCCCAATCCCGACCTTGTTTTCAATTGCCATCCATTCTTCATGCTCCTTGGTTTCGTGTTCATGTCGGGGGAAGCAATGATGGCATACAAGACAGTAAGGGCAGAAAAGAAAACAAAGAAGATAGTTCACATGGCTCTGCATTTTGTAGCCATTTGCATGGGAATTGCAGGGATAAACGCTGCTTTCAAGTTTCATGATCAGGTGAACTTGGAAGATATGTTTAGTTTACATTCATGGATTGGCATTGTCACTTTCTGCCTTTATGGCTTACAGTGGCTGTTGGGACTATGTACATTCTTGTTTCCAGGAGCAGATGCAGCGACTAAGGCCAGACTGCTGCCATGGCACATAAGTGGAGGAAGAGCTTTGCTGCTGATGATAATATGCACAGCCTTGACTGGCTTGTCTGAGAAGGCTACTTCTTTGAGACTGGCTGGCAACCATGAGTCTCGTTTGCTTAACTTCACTGCTTTCTTTGTCCTCCTCTTTGGCATTTCCATTGACCTCTCCATCGGTTTTGCTCGGTTTATGCACGCTGATCTCACCGTTTAG

Coding sequence (CDS)

ATGGACAGGGCAAGTTTCAGGCACACTGCTTCTGCAATTACTGTCTTTGCACATATTTTTGGTCTCACTGCCTGCATTCTTATGCTTGTTTGGTTGCTGCATTATCGTGGCGGCCTCGCCTACGATTCTCCCAATCCCGACCTTGTTTTCAATTGCCATCCATTCTTCATGCTCCTTGGTTTCGTGTTCATGTCGGGGGAAGCAATGATGGCATACAAGACAGTAAGGGCAGAAAAGAAAACAAAGAAGATAGTTCACATGGCTCTGCATTTTGTAGCCATTTGCATGGGAATTGCAGGGATAAACGCTGCTTTCAAGTTTCATGATCAGGTGAACTTGGAAGATATGTTTAGTTTACATTCATGGATTGGCATTGTCACTTTCTGCCTTTATGGCTTACAGTGGCTGTTGGGACTATGTACATTCTTGTTTCCAGGAGCAGATGCAGCGACTAAGGCCAGACTGCTGCCATGGCACATAAGTGGAGGAAGAGCTTTGCTGCTGATGATAATATGCACAGCCTTGACTGGCTTGTCTGAGAAGGCTACTTCTTTGAGACTGGCTGGCAACCATGAGTCTCGTTTGCTTAACTTCACTGCTTTCTTTGTCCTCCTCTTTGGCATTTCCATTGACCTCTCCATCGGTTTTGCTCGGTTTATGCACGCTGATCTCACCGTTTAG

Protein sequence

MDRASFRHTASAITVFAHIFGLTACILMLVWLLHYRGGLAYDSPNPDLVFNCHPFFMLLGFVFMSGEAMMAYKTVRAEKKTKKIVHMALHFVAICMGIAGINAAFKFHDQVNLEDMFSLHSWIGIVTFCLYGLQWLLGLCTFLFPGADAATKARLLPWHISGGRALLLMIICTALTGLSEKATSLRLAGNHESRLLNFTAFFVLLFGISIDLSIGFARFMHADLTV

Homology

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BLAST of HG10009588 vs. NCBI nr
Match: XP_038907208.1 (probable transmembrane ascorbate ferrireductase 3 [Benincasa hispida])

HSP 1 Score: 431.0 bits (1107), Expect = 6.2e-117
Identity = 214/226 (94.69%), Postives = 219/226 (96.90%), Query Frame = 0


Query: 1   MDRASFRHTASAITVFAHIFGLTACILMLVWLLHYRGGLAYDSPNPDLVFNCHPFFMLLG 60
           MDRA+FRHTAS+ITVFAHIFGLTACILMLVWLLHYRGGLAYDSPNPDLVFNCHPFFMLLG
Sbjct: 4   MDRATFRHTASSITVFAHIFGLTACILMLVWLLHYRGGLAYDSPNPDLVFNCHPFFMLLG 63

Query: 61  FVFMSGEAMMAYKTVRAEKKTKKIVHMALHFVAICMGIAGINAAFKFHDQVNLEDMFSLH 120
           FVFMSGEAMMAYKTVRAEK TKKIVHMALH VAICMGI GINAAFKFHDQVNLEDMFSLH
Sbjct: 64  FVFMSGEAMMAYKTVRAEKITKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMFSLH 123

Query: 121 SWIGIVTFCLYGLQWLLGLCTFLFPGADAATKARLLPWHISGGRALLLMIICTALTGLSE 180
           SWIGIVTFCLYGLQWLLGL TFLFPGADAATKARLLPWHISGGRALL+MIICTALTGLSE
Sbjct: 124 SWIGIVTFCLYGLQWLLGLFTFLFPGADAATKARLLPWHISGGRALLMMIICTALTGLSE 183

Query: 181 KATSLRLAGNHESRLLNFTAFFVLLFGISIDLSIGFARFMHADLTV 227
           KATSLRLAG+ ESRLLNFTAFFVLLFGISID+SIGFARFMH D TV
Sbjct: 184 KATSLRLAGSPESRLLNFTAFFVLLFGISIDVSIGFARFMHIDHTV 229

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BLAST of HG10009588 vs. NCBI nr
Match: XP_004142817.1 (probable transmembrane ascorbate ferrireductase 3 [Cucumis sativus] >KGN51448.1 hypothetical protein Csa_009397 [Cucumis sativus])

HSP 1 Score: 413.7 bits (1062), Expect = 1.0e-111
Identity = 201/226 (88.94%), Postives = 213/226 (94.25%), Query Frame = 0


Query: 1   MDRASFRHTASAITVFAHIFGLTACILMLVWLLHYRGGLAYDSPNPDLVFNCHPFFMLLG 60
           M+RA+FRHTAS ITVFAHIFGLTACIL LVWLLHYRGGLAYDSPN +LVFNCHPFFMLLG
Sbjct: 4   MERANFRHTASTITVFAHIFGLTACILTLVWLLHYRGGLAYDSPNSNLVFNCHPFFMLLG 63

Query: 61  FVFMSGEAMMAYKTVRAEKKTKKIVHMALHFVAICMGIAGINAAFKFHDQVNLEDMFSLH 120
           FVFMSGEAMMAY+TVRAEK+TKKIVHMALH VAICMGI GINAAFKFHDQVNLEDM+SLH
Sbjct: 64  FVFMSGEAMMAYRTVRAEKRTKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMYSLH 123

Query: 121 SWIGIVTFCLYGLQWLLGLCTFLFPGADAATKARLLPWHISGGRALLLMIICTALTGLSE 180
           SWIGIVTFCLYGLQW++GL TFLFPGAD  TK RLLPWHI+GGRALLLMIICTALTGLSE
Sbjct: 124 SWIGIVTFCLYGLQWVVGLFTFLFPGADRETKTRLLPWHITGGRALLLMIICTALTGLSE 183

Query: 181 KATSLRLAGNHESRLLNFTAFFVLLFGISIDLSIGFARFMHADLTV 227
           KATSLRL  +HESRLLNFTAFFVLLFGISID+SIGFARFMH D T+
Sbjct: 184 KATSLRLTSSHESRLLNFTAFFVLLFGISIDISIGFARFMHVDHTI 229

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BLAST of HG10009588 vs. NCBI nr
Match: XP_008466953.1 (PREDICTED: probable transmembrane ascorbate ferrireductase 3 [Cucumis melo])

HSP 1 Score: 408.7 bits (1049), Expect = 3.3e-110
Identity = 200/226 (88.50%), Postives = 210/226 (92.92%), Query Frame = 0


Query: 1   MDRASFRHTASAITVFAHIFGLTACILMLVWLLHYRGGLAYDSPNPDLVFNCHPFFMLLG 60
           M+RA+FRHTAS I VFAHI GLTACILMLVWLLHYRGGLAYDSP+ +LVFNCHPFFMLLG
Sbjct: 4   MERANFRHTASTIAVFAHISGLTACILMLVWLLHYRGGLAYDSPDANLVFNCHPFFMLLG 63

Query: 61  FVFMSGEAMMAYKTVRAEKKTKKIVHMALHFVAICMGIAGINAAFKFHDQVNLEDMFSLH 120
           FVFMSGEAMMAY+TVRAEK TKKIVHM LH VAICMGI GINAAFKFHDQVNLEDM+SLH
Sbjct: 64  FVFMSGEAMMAYRTVRAEKITKKIVHMGLHLVAICMGIIGINAAFKFHDQVNLEDMYSLH 123

Query: 121 SWIGIVTFCLYGLQWLLGLCTFLFPGADAATKARLLPWHISGGRALLLMIICTALTGLSE 180
           SWIGIVTFCLYGLQWLLGL TFLFPGADA TK RLLPWHISGGRALL+MIICTALTGLSE
Sbjct: 124 SWIGIVTFCLYGLQWLLGLFTFLFPGADAMTKTRLLPWHISGGRALLMMIICTALTGLSE 183

Query: 181 KATSLRLAGNHESRLLNFTAFFVLLFGISIDLSIGFARFMHADLTV 227
           KATSLRL G+HESRL NFTAFFVLLFGISID+SI FARFMH D T+
Sbjct: 184 KATSLRLTGSHESRLFNFTAFFVLLFGISIDISISFARFMHVDHTI 229

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BLAST of HG10009588 vs. NCBI nr
Match: XP_022991076.1 (probable transmembrane ascorbate ferrireductase 3 [Cucurbita maxima])

HSP 1 Score: 396.4 bits (1017), Expect = 1.7e-106
Identity = 193/225 (85.78%), Postives = 205/225 (91.11%), Query Frame = 0


Query: 1   MDRASFRHTASAITVFAHIFGLTACILMLVWLLHYRGGLAYDSPNPDLVFNCHPFFMLLG 60
           +DR  FRHTASAI V AHIFGLTACIL LVWLLHYRGGLAYDS + +LVFNCHPFFMLLG
Sbjct: 4   VDRPGFRHTASAIAVLAHIFGLTACILTLVWLLHYRGGLAYDSRSAELVFNCHPFFMLLG 63

Query: 61  FVFMSGEAMMAYKTVRAEKKTKKIVHMALHFVAICMGIAGINAAFKFHDQVNLEDMFSLH 120
           FVF+SGEAMMAYKTVRA+K T+KIVHMALHFVAICMGIAGINA+FKFHD + LE +FSLH
Sbjct: 64  FVFVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIAGINASFKFHDMIKLEHVFSLH 123

Query: 121 SWIGIVTFCLYGLQWLLGLCTFLFPGADAATKARLLPWHISGGRALLLMIICTALTGLSE 180
           SWIGIVTFCLYGLQWLLGLCTFLFPGA   TKARLLPWHISGGRALLLMIICTALTGLS+
Sbjct: 124 SWIGIVTFCLYGLQWLLGLCTFLFPGASRETKARLLPWHISGGRALLLMIICTALTGLSQ 183

Query: 181 KATSLRLAGNHESRLLNFTAFFVLLFGISIDLSIGFARFMHADLT 226
           KATS  L GNHESRLLNFT FFVLLFGISID++IGF RF HADLT
Sbjct: 184 KATSSTLTGNHESRLLNFTGFFVLLFGISIDIAIGFDRFRHADLT 228

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BLAST of HG10009588 vs. NCBI nr
Match: XP_022146393.1 (probable transmembrane ascorbate ferrireductase 3 [Momordica charantia])

HSP 1 Score: 390.2 bits (1001), Expect = 1.2e-104
Identity = 186/217 (85.71%), Postives = 205/217 (94.47%), Query Frame = 0


Query: 1   MDRASFRHTASAITVFAHIFGLTACILMLVWLLHYRGGLAYDSPNPDLVFNCHPFFMLLG 60
           M+RASFRHTASA+T+ AHIFG+TACIL+LVWLLHYRGGL YDSPNP+LVFNCHPFFMLLG
Sbjct: 4   MERASFRHTASAVTIMAHIFGVTACILLLVWLLHYRGGLGYDSPNPNLVFNCHPFFMLLG 63

Query: 61  FVFMSGEAMMAYKTVRAEKKTKKIVHMALHFVAICMGIAGINAAFKFHDQVNLEDMFSLH 120
           FVF+SGEAMMA+KTVRA+K  +KI HM LH VAICMGIAGINAAFKFHD VNLEDMFSLH
Sbjct: 64  FVFVSGEAMMAFKTVRADKMARKITHMGLHLVAICMGIAGINAAFKFHDLVNLEDMFSLH 123

Query: 121 SWIGIVTFCLYGLQWLLGLCTFLFPGADAATKARLLPWHISGGRALLLMIICTALTGLSE 180
           SWIGIVTF LYGLQW+LGLCTFLFPGA+A+TKA+LLPWHISGGRALLLMIICTALTGLS+
Sbjct: 124 SWIGIVTFSLYGLQWVLGLCTFLFPGAEASTKAKLLPWHISGGRALLLMIICTALTGLSQ 183

Query: 181 KATSLRLAGNHESRLLNFTAFFVLLFGISIDLSIGFA 218
           KATSL+L  NHESRLLNFTAFFVLLFGISI++ +GFA
Sbjct: 184 KATSLKLVANHESRLLNFTAFFVLLFGISINIVVGFA 220

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BLAST of HG10009588 vs. ExPASy Swiss-Prot
Match: Q67ZF6 (Probable transmembrane ascorbate ferrireductase 3 OS=Arabidopsis thaliana OX=3702 GN=CYB561C PE=2 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 1.4e-63
Identity = 115/218 (52.75%), Postives = 152/218 (69.72%), Query Frame = 0


Query: 2   DRASFRHTASAITVFAHIFGLTACILMLVWLLHYRGGLAYDSPNPDLVFNCHPFFMLLGF 61
           DR + +  +S  T+ AH FG+ A +LML+WLLHYR G+ Y S NP  V N HPF M  GF
Sbjct: 6   DRTTLKRHSSLSTLVAHFFGILAVVLMLIWLLHYREGIEYGSDNPLKVLNVHPFLMYCGF 65

Query: 62  VFMSGEAMMAYKTVRAEKKTKKIVHMALHFVAICMGIAGINAAFKFHDQVNLEDMFSLHS 121
           +F+ G+AMM YKT  A  + +K+VH  LH + + +GI GI AAF+FHD+VNL+DM SLHS
Sbjct: 66  LFLVGQAMMTYKTAYASHQVQKMVHGGLHLIGLVLGIVGICAAFRFHDKVNLKDMVSLHS 125

Query: 122 WIGIVTFCLYGLQWLLGLCTFLFPGADAATKARLLPWHISGGRALLLMIICTALTGLSEK 181
           WIG+ TF L G+QWL G  TFL P + + T+ R++PWH+ GGRALL M I  ALTGL ++
Sbjct: 126 WIGLTTFILLGVQWLFGAFTFLAPQSSSGTRTRMMPWHVLGGRALLYMGIVAALTGLMQR 185

Query: 182 ATSLRLAGNHESRLLNFTAFFVLLFGISIDLSIGFARF 220
           AT L  + N ESRL+NF    +LLFG+S+D S+   R+
Sbjct: 186 ATMLGQSTNAESRLINFLGLAILLFGVSVDFSVALGRY 223

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BLAST of HG10009588 vs. ExPASy Swiss-Prot
Match: Q8L856 (Transmembrane ascorbate ferrireductase 1 OS=Arabidopsis thaliana OX=3702 GN=CYB561A PE=1 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 7.8e-46
Identity = 90/211 (42.65%), Postives = 132/211 (62.56%), Query Frame = 0


Query: 7   RHTASAITVFAHIFGLTACILMLVWLLHYRGGLAYDSPNPDLVFNCHPFFMLLGFVFMSG 66
           R  A A+T  AH   + A I++LVW + YRGGLA+++ N +L+FN HP  ML+GF+ + G
Sbjct: 4   RINAMAVTFVAHALAVIAAIMVLVWSISYRGGLAWEATNKNLIFNLHPVLMLIGFIILGG 63

Query: 67  EAMMAYKTVRAEKKTKKIVHMALHFVAICMGIAGINAAFKFHDQVNLEDMFSLHSWIGIV 126
           EA+++YK++  EK  KK++H+ LH +A+ +GI GI AAFK H++ ++ +++SLHSWIGI 
Sbjct: 64  EAIISYKSLPLEKPVKKLIHLILHAIALALGIFGICAAFKNHNESHIPNLYSLHSWIGIG 123

Query: 127 TFCLYGLQWLLGLCTFLFPGADAATKARLLPWHISGGRALLLMIICTALTGLSEKATSLR 186
              LYG QW+     F FPG     K+ LLPWH   G  + ++ +  A  G  EK T L 
Sbjct: 124 VISLYGFQWVYSFIVFFFPGGSTNLKSGLLPWHAMLGLFVYILAVGNAALGFLEKLTFLE 183

Query: 187 LAG----NHESRLLNFTAFFVLLFGISIDLS 214
             G      E+ L+NFTA   +LFG  + L+
Sbjct: 184 NGGLDKYGSEAFLINFTAIITILFGAFVVLT 214

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BLAST of HG10009588 vs. ExPASy Swiss-Prot
Match: A3A9H6 (Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0642300 PE=3 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.3e-45
Identity = 87/206 (42.23%), Postives = 131/206 (63.59%), Query Frame = 0


Query: 10  ASAITVFAHIFGLTACILMLVWLLHYRGGLAYDSPNPDLVFNCHPFFMLLGFVFMSGEAM 69
           A+  T  AH   + A  ++LVW +H+RGGLA+++ N +L+FN HP  ML+G++ +  EA+
Sbjct: 7   AAPFTYVAHALAVAAATMVLVWCIHFRGGLAFEATNKNLIFNVHPVLMLIGYIILGSEAI 66

Query: 70  MAYKTVRAEK-KTKKIVHMALHFVAICMGIAGINAAFKFHDQVNLEDMFSLHSWIGIVTF 129
           M YK +   K  T K++H+ LH +A+  G  GI  AFKFH++  + +++SLHSW+GI T 
Sbjct: 67  MVYKVLPTWKHDTTKLIHLILHAIALVFGAVGIYCAFKFHNESGIANLYSLHSWLGIGTI 126

Query: 130 CLYGLQWLLGLCTFLFPGADAATKARLLPWHISGGRALLLMIICTALTGLSEKATSLRLA 189
           CLYG+QW+ G   F FP A  + +  +LPWHI  G  + ++ + TA  G  EK T L+ +
Sbjct: 127 CLYGIQWIFGFVAFFFPRASPSVRKGVLPWHILFGLFVYILALATAELGFLEKLTFLQSS 186

Query: 190 G----NHESRLLNFTAFFVLLFGISI 211
           G      E+ L+NFTA  V+LFG S+
Sbjct: 187 GLDKYGAEAFLVNFTALIVVLFGASV 212

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BLAST of HG10009588 vs. ExPASy Swiss-Prot
Match: C4IYS8 (Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 2.1e-43
Identity = 86/206 (41.75%), Postives = 131/206 (63.59%), Query Frame = 0


Query: 10  ASAITVFAHIFGLTACILMLVWLLHYRGGLAYDSPNPDLVFNCHPFFMLLGFVFMSGEAM 69
           A+  T  AH   + A  ++LVW +++RGGLA ++ N +L+FN HP  ML+G++ + GEA+
Sbjct: 9   AAPFTYAAHALAVAAAAMVLVWAIYFRGGLAIEATNKNLIFNVHPVLMLIGYIIIGGEAI 68

Query: 70  MAYKTV-RAEKKTKKIVHMALHFVAICMGIAGINAAFKFHDQVNLEDMFSLHSWIGIVTF 129
           M Y+ +  +  +T K++H+ LH +A+ +G  GI  AFK H++  + +++SLHSWIGI T 
Sbjct: 69  MVYRVLPTSNHETNKLIHLVLHGIALVLGAVGIYFAFKNHNESGIANLYSLHSWIGIGTI 128

Query: 130 CLYGLQWLLGLCTFLFPGADAATKARLLPWHISGGRALLLMIICTALTGLSEKATSLRLA 189
            LYG+QW++G  TF FPGA    K  +LPWHI  G  + ++ +  A  G  EK T L  +
Sbjct: 129 TLYGIQWIVGFVTFFFPGAAPNVKKGVLPWHILFGLFVYILALANAELGFLEKLTFLESS 188

Query: 190 G----NHESRLLNFTAFFVLLFGISI 211
           G      E+ L+NFTA  V+LFG S+
Sbjct: 189 GLDKYGTEAFLVNFTALVVVLFGASV 214

[Close]

BLAST of HG10009588 vs. ExPASy Swiss-Prot
Match: Q6I681 (Ascorbate-specific transmembrane electron transporter 1 OS=Zea mays OX=4577 GN=ZCYB PE=1 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 4.7e-43
Identity = 87/206 (42.23%), Postives = 129/206 (62.62%), Query Frame = 0


Query: 10  ASAITVFAHIFGLTACILMLVWLLHYRGGLAYDSPNPDLVFNCHPFFMLLGFVFMSGEAM 69
           A+  T  AH   + A  ++LVW + +RGGLA +S N +L+FN HP  ML+G+V + GEA+
Sbjct: 9   AAPFTYAAHALAVAAAAMVLVWSIQFRGGLAIESTNKNLIFNVHPVLMLIGYVIIGGEAI 68

Query: 70  MAYKTV-RAEKKTKKIVHMALHFVAICMGIAGINAAFKFHDQVNLEDMFSLHSWIGIVTF 129
           M Y+ +  +   T K++H+ LH +A+ +G  GI  AFK H++  + +++SLHSWIGI T 
Sbjct: 69  MVYRVLPTSNHDTTKLIHLILHGIALVLGAVGIYFAFKNHNESGIANLYSLHSWIGIGTI 128

Query: 130 CLYGLQWLLGLCTFLFPGADAATKARLLPWHISGGRALLLMIICTALTGLSEKATSLRLA 189
            LYG+QW++G  TF FPGA    K  +LPWH+  G  + ++ +  A  G  EK T L  +
Sbjct: 129 TLYGIQWIIGFVTFFFPGAAPNVKKGVLPWHVLFGLFVYILALANAELGFLEKLTFLESS 188

Query: 190 G----NHESRLLNFTAFFVLLFGISI 211
           G      E+ L+NFTA  V+LFG S+
Sbjct: 189 GLDKYGTEAFLVNFTALVVVLFGASV 214

[Close]

BLAST of HG10009588 vs. ExPASy TrEMBL
Match: A0A0A0KPB5 (Cytochrome b561 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G548140 PE=4 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 5.0e-112
Identity = 201/226 (88.94%), Postives = 213/226 (94.25%), Query Frame = 0


Query: 1   MDRASFRHTASAITVFAHIFGLTACILMLVWLLHYRGGLAYDSPNPDLVFNCHPFFMLLG 60
           M+RA+FRHTAS ITVFAHIFGLTACIL LVWLLHYRGGLAYDSPN +LVFNCHPFFMLLG
Sbjct: 4   MERANFRHTASTITVFAHIFGLTACILTLVWLLHYRGGLAYDSPNSNLVFNCHPFFMLLG 63

Query: 61  FVFMSGEAMMAYKTVRAEKKTKKIVHMALHFVAICMGIAGINAAFKFHDQVNLEDMFSLH 120
           FVFMSGEAMMAY+TVRAEK+TKKIVHMALH VAICMGI GINAAFKFHDQVNLEDM+SLH
Sbjct: 64  FVFMSGEAMMAYRTVRAEKRTKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMYSLH 123

Query: 121 SWIGIVTFCLYGLQWLLGLCTFLFPGADAATKARLLPWHISGGRALLLMIICTALTGLSE 180
           SWIGIVTFCLYGLQW++GL TFLFPGAD  TK RLLPWHI+GGRALLLMIICTALTGLSE
Sbjct: 124 SWIGIVTFCLYGLQWVVGLFTFLFPGADRETKTRLLPWHITGGRALLLMIICTALTGLSE 183

Query: 181 KATSLRLAGNHESRLLNFTAFFVLLFGISIDLSIGFARFMHADLTV 227
           KATSLRL  +HESRLLNFTAFFVLLFGISID+SIGFARFMH D T+
Sbjct: 184 KATSLRLTSSHESRLLNFTAFFVLLFGISIDISIGFARFMHVDHTI 229

[Close]

BLAST of HG10009588 vs. ExPASy TrEMBL
Match: A0A1S3CSD9 (probable transmembrane ascorbate ferrireductase 3 OS=Cucumis melo OX=3656 GN=LOC103504338 PE=4 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 1.6e-110
Identity = 200/226 (88.50%), Postives = 210/226 (92.92%), Query Frame = 0


Query: 1   MDRASFRHTASAITVFAHIFGLTACILMLVWLLHYRGGLAYDSPNPDLVFNCHPFFMLLG 60
           M+RA+FRHTAS I VFAHI GLTACILMLVWLLHYRGGLAYDSP+ +LVFNCHPFFMLLG
Sbjct: 4   MERANFRHTASTIAVFAHISGLTACILMLVWLLHYRGGLAYDSPDANLVFNCHPFFMLLG 63

Query: 61  FVFMSGEAMMAYKTVRAEKKTKKIVHMALHFVAICMGIAGINAAFKFHDQVNLEDMFSLH 120
           FVFMSGEAMMAY+TVRAEK TKKIVHM LH VAICMGI GINAAFKFHDQVNLEDM+SLH
Sbjct: 64  FVFMSGEAMMAYRTVRAEKITKKIVHMGLHLVAICMGIIGINAAFKFHDQVNLEDMYSLH 123

Query: 121 SWIGIVTFCLYGLQWLLGLCTFLFPGADAATKARLLPWHISGGRALLLMIICTALTGLSE 180
           SWIGIVTFCLYGLQWLLGL TFLFPGADA TK RLLPWHISGGRALL+MIICTALTGLSE
Sbjct: 124 SWIGIVTFCLYGLQWLLGLFTFLFPGADAMTKTRLLPWHISGGRALLMMIICTALTGLSE 183

Query: 181 KATSLRLAGNHESRLLNFTAFFVLLFGISIDLSIGFARFMHADLTV 227
           KATSLRL G+HESRL NFTAFFVLLFGISID+SI FARFMH D T+
Sbjct: 184 KATSLRLTGSHESRLFNFTAFFVLLFGISIDISISFARFMHVDHTI 229

[Close]

BLAST of HG10009588 vs. ExPASy TrEMBL
Match: A0A6J1JPQ6 (probable transmembrane ascorbate ferrireductase 3 OS=Cucurbita maxima OX=3661 GN=LOC111487782 PE=4 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 8.2e-107
Identity = 193/225 (85.78%), Postives = 205/225 (91.11%), Query Frame = 0


Query: 1   MDRASFRHTASAITVFAHIFGLTACILMLVWLLHYRGGLAYDSPNPDLVFNCHPFFMLLG 60
           +DR  FRHTASAI V AHIFGLTACIL LVWLLHYRGGLAYDS + +LVFNCHPFFMLLG
Sbjct: 4   VDRPGFRHTASAIAVLAHIFGLTACILTLVWLLHYRGGLAYDSRSAELVFNCHPFFMLLG 63

Query: 61  FVFMSGEAMMAYKTVRAEKKTKKIVHMALHFVAICMGIAGINAAFKFHDQVNLEDMFSLH 120
           FVF+SGEAMMAYKTVRA+K T+KIVHMALHFVAICMGIAGINA+FKFHD + LE +FSLH
Sbjct: 64  FVFVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIAGINASFKFHDMIKLEHVFSLH 123

Query: 121 SWIGIVTFCLYGLQWLLGLCTFLFPGADAATKARLLPWHISGGRALLLMIICTALTGLSE 180
           SWIGIVTFCLYGLQWLLGLCTFLFPGA   TKARLLPWHISGGRALLLMIICTALTGLS+
Sbjct: 124 SWIGIVTFCLYGLQWLLGLCTFLFPGASRETKARLLPWHISGGRALLLMIICTALTGLSQ 183

Query: 181 KATSLRLAGNHESRLLNFTAFFVLLFGISIDLSIGFARFMHADLT 226
           KATS  L GNHESRLLNFT FFVLLFGISID++IGF RF HADLT
Sbjct: 184 KATSSTLTGNHESRLLNFTGFFVLLFGISIDIAIGFDRFRHADLT 228

[Close]

BLAST of HG10009588 vs. ExPASy TrEMBL
Match: A0A6J1CYG4 (probable transmembrane ascorbate ferrireductase 3 OS=Momordica charantia OX=3673 GN=LOC111015617 PE=4 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 5.9e-105
Identity = 186/217 (85.71%), Postives = 205/217 (94.47%), Query Frame = 0


Query: 1   MDRASFRHTASAITVFAHIFGLTACILMLVWLLHYRGGLAYDSPNPDLVFNCHPFFMLLG 60
           M+RASFRHTASA+T+ AHIFG+TACIL+LVWLLHYRGGL YDSPNP+LVFNCHPFFMLLG
Sbjct: 4   MERASFRHTASAVTIMAHIFGVTACILLLVWLLHYRGGLGYDSPNPNLVFNCHPFFMLLG 63

Query: 61  FVFMSGEAMMAYKTVRAEKKTKKIVHMALHFVAICMGIAGINAAFKFHDQVNLEDMFSLH 120
           FVF+SGEAMMA+KTVRA+K  +KI HM LH VAICMGIAGINAAFKFHD VNLEDMFSLH
Sbjct: 64  FVFVSGEAMMAFKTVRADKMARKITHMGLHLVAICMGIAGINAAFKFHDLVNLEDMFSLH 123

Query: 121 SWIGIVTFCLYGLQWLLGLCTFLFPGADAATKARLLPWHISGGRALLLMIICTALTGLSE 180
           SWIGIVTF LYGLQW+LGLCTFLFPGA+A+TKA+LLPWHISGGRALLLMIICTALTGLS+
Sbjct: 124 SWIGIVTFSLYGLQWVLGLCTFLFPGAEASTKAKLLPWHISGGRALLLMIICTALTGLSQ 183

Query: 181 KATSLRLAGNHESRLLNFTAFFVLLFGISIDLSIGFA 218
           KATSL+L  NHESRLLNFTAFFVLLFGISI++ +GFA
Sbjct: 184 KATSLKLVANHESRLLNFTAFFVLLFGISINIVVGFA 220

[Close]

BLAST of HG10009588 vs. ExPASy TrEMBL
Match: A0A6J1HF52 (probable transmembrane ascorbate ferrireductase 3 OS=Cucurbita moschata OX=3662 GN=LOC111462470 PE=4 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 4.2e-103
Identity = 186/225 (82.67%), Postives = 200/225 (88.89%), Query Frame = 0


Query: 1   MDRASFRHTASAITVFAHIFGLTACILMLVWLLHYRGGLAYDSPNPDLVFNCHPFFMLLG 60
           ++R  FRHTASAI   AHIFGLTACIL LVWLLHYRGGLAYDS + DLVFNCHPFFMLLG
Sbjct: 4   VNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFMLLG 63

Query: 61  FVFMSGEAMMAYKTVRAEKKTKKIVHMALHFVAICMGIAGINAAFKFHDQVNLEDMFSLH 120
           FV +SGEAMMAYKTVRA+K T+KIVHMALHFVAICMGI GINA+FKFHD + LE MFS+H
Sbjct: 64  FVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMFSIH 123

Query: 121 SWIGIVTFCLYGLQWLLGLCTFLFPGADAATKARLLPWHISGGRALLLMIICTALTGLSE 180
           SWIGIVTFCLYGLQWLLGLCTFLFPGA + TKARLLPWHISGGRALLLMIICTALTGLS+
Sbjct: 124 SWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTGLSQ 183

Query: 181 KATSLRLAGNHESRLLNFTAFFVLLFGISIDLSIGFARFMHADLT 226
           KATS  L GNHE+RLL FT FFVLLFGISID++IGF RF H D T
Sbjct: 184 KATSSTLTGNHEARLLKFTGFFVLLFGISIDIAIGFDRFRHVDPT 228

[Close]

BLAST of HG10009588 vs. TAIR 10
Match: AT1G14730.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 244.2 bits (622), Expect = 1.0e-64
Identity = 115/218 (52.75%), Postives = 152/218 (69.72%), Query Frame = 0


Query: 2   DRASFRHTASAITVFAHIFGLTACILMLVWLLHYRGGLAYDSPNPDLVFNCHPFFMLLGF 61
           DR + +  +S  T+ AH FG+ A +LML+WLLHYR G+ Y S NP  V N HPF M  GF
Sbjct: 6   DRTTLKRHSSLSTLVAHFFGILAVVLMLIWLLHYREGIEYGSDNPLKVLNVHPFLMYCGF 65

Query: 62  VFMSGEAMMAYKTVRAEKKTKKIVHMALHFVAICMGIAGINAAFKFHDQVNLEDMFSLHS 121
           +F+ G+AMM YKT  A  + +K+VH  LH + + +GI GI AAF+FHD+VNL+DM SLHS
Sbjct: 66  LFLVGQAMMTYKTAYASHQVQKMVHGGLHLIGLVLGIVGICAAFRFHDKVNLKDMVSLHS 125

Query: 122 WIGIVTFCLYGLQWLLGLCTFLFPGADAATKARLLPWHISGGRALLLMIICTALTGLSEK 181
           WIG+ TF L G+QWL G  TFL P + + T+ R++PWH+ GGRALL M I  ALTGL ++
Sbjct: 126 WIGLTTFILLGVQWLFGAFTFLAPQSSSGTRTRMMPWHVLGGRALLYMGIVAALTGLMQR 185

Query: 182 ATSLRLAGNHESRLLNFTAFFVLLFGISIDLSIGFARF 220
           AT L  + N ESRL+NF    +LLFG+S+D S+   R+
Sbjct: 186 ATMLGQSTNAESRLINFLGLAILLFGVSVDFSVALGRY 223

[Close]

BLAST of HG10009588 vs. TAIR 10
Match: AT4G25570.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 185.3 bits (469), Expect = 5.5e-47
Identity = 90/211 (42.65%), Postives = 132/211 (62.56%), Query Frame = 0


Query: 7   RHTASAITVFAHIFGLTACILMLVWLLHYRGGLAYDSPNPDLVFNCHPFFMLLGFVFMSG 66
           R  A A+T  AH   + A I++LVW + YRGGLA+++ N +L+FN HP  ML+GF+ + G
Sbjct: 4   RINAMAVTFVAHALAVIAAIMVLVWSISYRGGLAWEATNKNLIFNLHPVLMLIGFIILGG 63

Query: 67  EAMMAYKTVRAEKKTKKIVHMALHFVAICMGIAGINAAFKFHDQVNLEDMFSLHSWIGIV 126
           EA+++YK++  EK  KK++H+ LH +A+ +GI GI AAFK H++ ++ +++SLHSWIGI 
Sbjct: 64  EAIISYKSLPLEKPVKKLIHLILHAIALALGIFGICAAFKNHNESHIPNLYSLHSWIGIG 123

Query: 127 TFCLYGLQWLLGLCTFLFPGADAATKARLLPWHISGGRALLLMIICTALTGLSEKATSLR 186
              LYG QW+     F FPG     K+ LLPWH   G  + ++ +  A  G  EK T L 
Sbjct: 124 VISLYGFQWVYSFIVFFFPGGSTNLKSGLLPWHAMLGLFVYILAVGNAALGFLEKLTFLE 183

Query: 187 LAG----NHESRLLNFTAFFVLLFGISIDLS 214
             G      E+ L+NFTA   +LFG  + L+
Sbjct: 184 NGGLDKYGSEAFLINFTAIITILFGAFVVLT 214

[Close]

BLAST of HG10009588 vs. TAIR 10
Match: AT5G38630.1 (cytochrome B561-1 )

HSP 1 Score: 159.5 bits (402), Expect = 3.3e-39
Identity = 76/207 (36.71%), Postives = 119/207 (57.49%), Query Frame = 0


Query: 13  ITVFAHIFGLTACILMLVWLLHYRGGLAYDSPNPDLVFNCHPFFMLLGFVFMSGEAMMAY 72
           I +   + G     L+L W +HYRGGLA  S N D +FN HP  M++G +  +GEAM+AY
Sbjct: 11  IFMVVRVLGFIIAALVLTWTVHYRGGLALSSDNKDHIFNVHPVMMVIGLILFNGEAMLAY 70

Query: 73  KTVRAEKKTKKIVHMALHFVAICMGIAGINAAFKFHDQVNLEDMFSLHSWIGIVTFCLYG 132
           K+V+  K  KK+VH+ L   A  + + G+ AA KFH    +E+ +SLHSW+G+    L+ 
Sbjct: 71  KSVQGTKNLKKLVHLTLQLTAFILSLIGVWAALKFHIDKGIENFYSLHSWLGLACLFLFA 130

Query: 133 LQWLLGLCTFLFPGADAATKARLLPWHISGGRALLLMIICTALTGLSEKATSLRL----- 192
            QW  G  T+ +PG    ++A L+PWH+  G ++  + + TA TG+ EK T L++     
Sbjct: 131 FQWAAGFVTYWYPGGSRNSRASLMPWHVFLGISIYALALVTATTGILEKVTFLQVNQVIT 190

Query: 193 AGNHESRLLNFTAFFVLLFGISIDLSI 215
             + E+ L+N     +L+ G  + L +
Sbjct: 191 RYSTEAMLVNTMGVLILILGGFVILGV 217

[Close]

BLAST of HG10009588 vs. TAIR 10
Match: AT1G26100.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 105.9 bits (263), Expect = 4.3e-23
Identity = 60/180 (33.33%), Postives = 96/180 (53.33%), Query Frame = 0


Query: 12  AITVFAHIFGLTACILMLVWLLHYRG-GLAYDSPNPDLVFNCHPFFMLLGFVFMSGEAMM 71
           ++ +FA + GL   + +L W L     GL+Y +         HP  M++GF+ +SGEA++
Sbjct: 10  SLVLFARLSGLVVAVSVLYWALFLPNLGLSYST--------LHPLLMVIGFILVSGEAIL 69

Query: 72  AYKTVRAEKKTKKIVHMALHFVAICMGIAGINAAFKFHDQVNLEDMFSLHSWIGIVTFCL 131
            ++ +   +KTKK VH+ L  +A+   + GI   F +   V   + +SLHSW+G+++  L
Sbjct: 70  IHRWLPGSRKTKKAVHLWLQGMALASAVFGIWTKFHYQRGV-FANFYSLHSWMGLLSVSL 129

Query: 132 YGLQWLLGLCTFLFPGADAATKARLLPWHISGGRALLLMIICTALTGLSEKATSLRLAGN 191
           +  QW+ G  +F   G    T+   LPWH+  G     + I TA TGL EK T L+   N
Sbjct: 130 FAAQWVTGFMSFWHRGEVRTTRTTFLPWHVFLGLYTYGLAIATAETGLLEKLTFLQTKRN 180

The following BLAST results are available for this feature:

BLAST of HG10009588 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038907208.1	6.2e-117	94.69	probable transmembrane ascorbate ferrireductase 3 [Benincasa hispida]	[more]
XP_004142817.1	1.0e-111	88.94	probable transmembrane ascorbate ferrireductase 3 [Cucumis sativus] >KGN51448.1 ...	[more]
XP_008466953.1	3.3e-110	88.50	PREDICTED: probable transmembrane ascorbate ferrireductase 3 [Cucumis melo]	[more]
XP_022991076.1	1.7e-106	85.78	probable transmembrane ascorbate ferrireductase 3 [Cucurbita maxima]	[more]
XP_022146393.1	1.2e-104	85.71	probable transmembrane ascorbate ferrireductase 3 [Momordica charantia]	[more]

BLAST of HG10009588 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q67ZF6	1.4e-63	52.75	Probable transmembrane ascorbate ferrireductase 3 OS=Arabidopsis thaliana OX=370...	[more]
Q8L856	7.8e-46	42.65	Transmembrane ascorbate ferrireductase 1 OS=Arabidopsis thaliana OX=3702 GN=CYB5...	[more]
A3A9H6	1.3e-45	42.23	Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa...	[more]
C4IYS8	2.1e-43	41.75	Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2...	[more]
Q6I681	4.7e-43	42.23	Ascorbate-specific transmembrane electron transporter 1 OS=Zea mays OX=4577 GN=Z...	[more]

BLAST of HG10009588 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KPB5	5.0e-112	88.94	Cytochrome b561 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G54...	[more]
A0A1S3CSD9	1.6e-110	88.50	probable transmembrane ascorbate ferrireductase 3 OS=Cucumis melo OX=3656 GN=LOC...	[more]
A0A6J1JPQ6	8.2e-107	85.78	probable transmembrane ascorbate ferrireductase 3 OS=Cucurbita maxima OX=3661 GN...	[more]
A0A6J1CYG4	5.9e-105	85.71	probable transmembrane ascorbate ferrireductase 3 OS=Momordica charantia OX=3673...	[more]
A0A6J1HF52	4.2e-103	82.67	probable transmembrane ascorbate ferrireductase 3 OS=Cucurbita moschata OX=3662 ...	[more]

BLAST of HG10009588 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G14730.1	1.0e-64	52.75	Cytochrome b561/ferric reductase transmembrane protein family	[more]
AT4G25570.1	5.5e-47	42.65	Cytochrome b561/ferric reductase transmembrane protein family	[more]
AT5G38630.1	3.3e-39	36.71	cytochrome B561-1	[more]
AT1G26100.1	4.3e-23	33.33	Cytochrome b561/ferric reductase transmembrane protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006593	Cytochrome b561/ferric reductase transmembrane	SMART	SM00665	561_7	coord: 52..178 e-value: 7.8E-48 score: 174.9
IPR006593	Cytochrome b561/ferric reductase transmembrane	PFAM	PF03188	Cytochrom_B561	coord: 53..183 e-value: 1.1E-39 score: 135.7
IPR006593	Cytochrome b561/ferric reductase transmembrane	PROSITE	PS50939	CYTOCHROME_B561	coord: 16..215 score: 43.035767
None	No IPR available	GENE3D	1.20.120.1770		coord: 6..217 e-value: 1.1E-68 score: 232.8
None	No IPR available	PANTHER	PTHR10106:SF15	TRANSMEMBRANE ASCORBATE FERRIREDUCTASE 3-RELATED	coord: 3..217
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..25 score: 6.0
None	No IPR available	CDD	cd08766	Cyt_b561_ACYB-1_like	coord: 43..185 e-value: 4.20811E-65 score: 196.374
IPR043205	Cytochrome b561/Cytochrome b reductase 1-like	PANTHER	PTHR10106	CYTOCHROME B561-RELATED	coord: 3..217

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10009588.1	HG10009588.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016491	oxidoreductase activity