HG10009470 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10009470 vs. NCBI nr
Match: XP_038875929.1 (valine--tRNA ligase, mitochondrial 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 2051.6 bits (5314), Expect = 0.0e+00
Identity = 1011/1046 (96.65%), Postives = 1029/1046 (98.37%), Query Frame = 0

Query: 1    MESDKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAAPKKSEKKNARRG 60
            ME +KKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSN APKKSEKKNARRG
Sbjct: 1    MEPEKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSN-APKKSEKKNARRG 60

Query: 61   GDEENAEDFVDPDTPFGKKKLLSQQMAKQYNPSAVEKSWYEWWEKSEFFVADAKSSKPPF 120
            GDEENAEDFVDPDT FGKKKLLSQQMAKQYNPSAVEKSWYEWWEKS FFVADAKSSKPPF
Sbjct: 61   GDEENAEDFVDPDTSFGKKKLLSQQMAKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPF 120

Query: 121  VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM 180
            VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM
Sbjct: 121  VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM 180

Query: 181  RERNLTRHDIGREKFISEVWDWKTKYGGTILRQLRRLGASLDWTRECFTMDEKRSRAVTE 240
            RERNLTRHDIGREKF+SEVWDWKTKYGGTIL+QLRRLGASLDWTRECFTMDEKRSRAVTE
Sbjct: 181  RERNLTRHDIGREKFMSEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTE 240

Query: 241  AFVRLFKNGVIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTS 300
            AFVRLFKNG+IYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLL+VPGYENPVEFGVLTS
Sbjct: 241  AFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLRVPGYENPVEFGVLTS 300

Query: 301  FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA 360
            FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA
Sbjct: 301  FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA 360

Query: 361  ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAR 420
            ILVDP FGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAR
Sbjct: 361  ILVDPKFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAR 420

Query: 421  EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDPAKQSLDAAMDNE 480
            E+VVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDPAKQSLDAAMDNE
Sbjct: 421  EVVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDPAKQSLDAAMDNE 480

Query: 481  CKKLDIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWV 540
            CKKL+IIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWV
Sbjct: 481  CKKLEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWV 540

Query: 541  VARNEEEAQEEARQIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTS 600
            VAR EEEA+E+ARQIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLK FYPTS
Sbjct: 541  VARTEEEAEEDARQIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKTFYPTS 600

Query: 601  ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI 660
            ALETGHDILFFWVARMVMLGI LGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI
Sbjct: 601  ALETGHDILFFWVARMVMLGIVLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI 660

Query: 661  NGISLEGLHKRLEEGNLDPKELAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN 720
            NG+SLEGLHKRLEEGNLDPKELAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN
Sbjct: 661  NGVSLEGLHKRLEEGNLDPKELAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN 720

Query: 721  LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPNKNVTSDVLPFSCQWILSVLNKAISRT 780
            LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPNKNVT DVLPFSCQWILSVLNKAISRT
Sbjct: 721  LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPNKNVTPDVLPFSCQWILSVLNKAISRT 780

Query: 781  VSSLESYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEFASARSHAQDTLWLCLEN 840
            +SSLESYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSND EFAS RSHAQDTLWLCLEN
Sbjct: 781  ISSLESYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDAEFASVRSHAQDTLWLCLEN 840

Query: 841  GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVIEEWTNEAVENEMDLILSAV 900
            GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYP VIEEWTNEAVENEMDLI+SAV
Sbjct: 841  GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPLVIEEWTNEAVENEMDLIVSAV 900

Query: 901  KSLRSLAKESRERRPGYVLPRNDAVAEYINKRKLEIVTLANLSSLTVINDNDAAPIGCAV 960
            KSLRSLAKESRERRPGYV PRNDAVAE INKRKLEIVTLANLSSLTVINDNDAAP+GC +
Sbjct: 901  KSLRSLAKESRERRPGYVFPRNDAVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCTI 960

Query: 961  SVVNENLSVYLQFQGAVSVEAELEKIKKKVEEIKKQQEKLKKMMDASGYKEKVRPHIHEE 1020
            SVVNENLSVYLQFQGA+S EAELEKIKKK++EIKKQQEKLKKMMDA+GYKEKVRPHIHEE
Sbjct: 961  SVVNENLSVYLQFQGAISAEAELEKIKKKMDEIKKQQEKLKKMMDANGYKEKVRPHIHEE 1020

Query: 1021 NVNKLASLMQELLSLEEAGQHIQAHR 1047
            NVNKLASLMQELLSLEEAGQHIQA R
Sbjct: 1021 NVNKLASLMQELLSLEEAGQHIQAQR 1045

BLAST of HG10009470 vs. NCBI nr
Match: XP_008467213.1 (PREDICTED: valine--tRNA ligase, mitochondrial 1 [Cucumis melo])

HSP 1 Score: 2046.9 bits (5302), Expect = 0.0e+00
Identity = 1005/1046 (96.08%), Postives = 1034/1046 (98.85%), Query Frame = 0

Query: 1    MESDKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAAPKKSEKKNARRG 60
            ME DKKPE+EDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSN APKKSEKKNARRG
Sbjct: 1    MEPDKKPEMEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSN-APKKSEKKNARRG 60

Query: 61   GDEENAEDFVDPDTPFGKKKLLSQQMAKQYNPSAVEKSWYEWWEKSEFFVADAKSSKPPF 120
            GDEENAEDFVDPDTPFGKKKLLSQQMAKQYNPSAVEKSWYEWWEKS +FVADAKSSKPPF
Sbjct: 61   GDEENAEDFVDPDTPFGKKKLLSQQMAKQYNPSAVEKSWYEWWEKSGYFVADAKSSKPPF 120

Query: 121  VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM 180
            VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSG+NTLWVPGTDHAGIATQVVVEKKIM
Sbjct: 121  VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGFNTLWVPGTDHAGIATQVVVEKKIM 180

Query: 181  RERNLTRHDIGREKFISEVWDWKTKYGGTILRQLRRLGASLDWTRECFTMDEKRSRAVTE 240
            RERNLTRHDIGREKFISEVW+WKTKYGGTIL+QLRRLGASLDWTRECFTMDEKRSRAVTE
Sbjct: 181  RERNLTRHDIGREKFISEVWEWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTE 240

Query: 241  AFVRLFKNGVIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTS 300
            AFVRLFKNG+IYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLL+VPGYENPVEFGVLTS
Sbjct: 241  AFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLRVPGYENPVEFGVLTS 300

Query: 301  FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA 360
            FAYPLEGELGEIVVATTRVETMLGDTAIA+HP+DTRYKHLHGKFAIHPFNGRKLPIICDA
Sbjct: 301  FAYPLEGELGEIVVATTRVETMLGDTAIAVHPEDTRYKHLHGKFAIHPFNGRKLPIICDA 360

Query: 361  ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAR 420
            ILVDP FGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAR
Sbjct: 361  ILVDPKFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAR 420

Query: 421  EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDPAKQSLDAAMDNE 480
            EMVVDALQKKGLYRGAKDNEMRLGLCSR+NDVVEPMIKPQWYVNCK+PAKQSLDAAMDNE
Sbjct: 421  EMVVDALQKKGLYRGAKDNEMRLGLCSRTNDVVEPMIKPQWYVNCKEPAKQSLDAAMDNE 480

Query: 481  CKKLDIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWV 540
            CKK+DIIPKQYSADWKRWL+NIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWV
Sbjct: 481  CKKIDIIPKQYSADWKRWLDNIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWV 540

Query: 541  VARNEEEAQEEARQIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTS 600
            VARNEEEA+EEA++IYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTS
Sbjct: 541  VARNEEEAEEEAKRIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTS 600

Query: 601  ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI 660
            ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI
Sbjct: 601  ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI 660

Query: 661  NGISLEGLHKRLEEGNLDPKELAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN 720
            NGISLEGLHKRLEEGNLDPKELAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN
Sbjct: 661  NGISLEGLHKRLEEGNLDPKELAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN 720

Query: 721  LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPNKNVTSDVLPFSCQWILSVLNKAISRT 780
            LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPNKN T DVLPFSCQWILSVLNKAISRT
Sbjct: 721  LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPNKNATPDVLPFSCQWILSVLNKAISRT 780

Query: 781  VSSLESYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEFASARSHAQDTLWLCLEN 840
            +SSLESYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSN+T++ASARSHAQDTLWLCLEN
Sbjct: 781  ISSLESYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNETDYASARSHAQDTLWLCLEN 840

Query: 841  GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVIEEWTNEAVENEMDLILSAV 900
            GLRLLHPFMPYVTEELWQRLPYPK+STRPESIMICDYPSV EEWTNEAVENEMDLI+SAV
Sbjct: 841  GLRLLHPFMPYVTEELWQRLPYPKNSTRPESIMICDYPSVTEEWTNEAVENEMDLIVSAV 900

Query: 901  KSLRSLAKESRERRPGYVLPRNDAVAEYINKRKLEIVTLANLSSLTVINDNDAAPIGCAV 960
            +SLRSLAKESRER+PGYVLPRN AVAE INKRKLEIVTLANLSSLTVINDNDAAP+GCAV
Sbjct: 901  RSLRSLAKESRERKPGYVLPRNLAVAEIINKRKLEIVTLANLSSLTVINDNDAAPVGCAV 960

Query: 961  SVVNENLSVYLQFQGAVSVEAELEKIKKKVEEIKKQQEKLKKMMDASGYKEKVRPHIHEE 1020
            SVVNENLSVYLQFQGA+S EAELEKI KK++EIKKQQE+LKKMMDASGYKEKVRP IHEE
Sbjct: 961  SVVNENLSVYLQFQGAISAEAELEKINKKIDEIKKQQERLKKMMDASGYKEKVRPQIHEE 1020

Query: 1021 NVNKLASLMQELLSLEEAGQHIQAHR 1047
            NVNKLASLMQELLSLEEAGQHIQAHR
Sbjct: 1021 NVNKLASLMQELLSLEEAGQHIQAHR 1045

BLAST of HG10009470 vs. NCBI nr
Match: KAG6605705.1 (Valine--tRNA ligase, mitochondrial 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2045.8 bits (5299), Expect = 0.0e+00
Identity = 1007/1046 (96.27%), Postives = 1029/1046 (98.37%), Query Frame = 0

Query: 1    MESDKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAAPKKSEKKNARRG 60
            MES KKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSN APKKSEKKNARRG
Sbjct: 44   MESVKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSN-APKKSEKKNARRG 103

Query: 61   GDEENAEDFVDPDTPFGKKKLLSQQMAKQYNPSAVEKSWYEWWEKSEFFVADAKSSKPPF 120
            GDEENAEDFVDPDTPFG KKL+SQQM+KQYNPSAVEKSWYEWWEKS FFVADAKSSKPPF
Sbjct: 104  GDEENAEDFVDPDTPFGNKKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPF 163

Query: 121  VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM 180
            VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM
Sbjct: 164  VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM 223

Query: 181  RERNLTRHDIGREKFISEVWDWKTKYGGTILRQLRRLGASLDWTRECFTMDEKRSRAVTE 240
            RERNLTRHDIGREKFISEVWDWKTKYGGTIL+QLRRLGASLDWTRECFTMDEKRSRAVTE
Sbjct: 224  RERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTE 283

Query: 241  AFVRLFKNGVIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTS 300
            AFVRLFKNG+IYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYE PVEFGVLTS
Sbjct: 284  AFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTS 343

Query: 301  FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA 360
            FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA
Sbjct: 344  FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA 403

Query: 361  ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAR 420
            ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEF GMPRFK R
Sbjct: 404  ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKVR 463

Query: 421  EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDPAKQSLDAAMDNE 480
            EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKD AKQSLDA MDNE
Sbjct: 464  EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVMDNE 523

Query: 481  CKKLDIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWV 540
            CKK++IIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATL+DDQLKEFGAYNDHWV
Sbjct: 524  CKKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWV 583

Query: 541  VARNEEEAQEEARQIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTS 600
            VARNEEEA+EEA+ IYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPD+TEDLKAFYPTS
Sbjct: 584  VARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTS 643

Query: 601  ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI 660
            ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI
Sbjct: 644  ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI 703

Query: 661  NGISLEGLHKRLEEGNLDPKELAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN 720
            NGISLEGLHKRLEEGNLDPKELA+AKEGQVKDFPNGISECGADALRFALISYTAQSDKIN
Sbjct: 704  NGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN 763

Query: 721  LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPNKNVTSDVLPFSCQWILSVLNKAISRT 780
            LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVP+KNVT D LPFSCQWILSVLNKAISRT
Sbjct: 764  LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISRT 823

Query: 781  VSSLESYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEFASARSHAQDTLWLCLEN 840
            VSSLE+YEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTE+ASARSHAQDTLWLCLEN
Sbjct: 824  VSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEYASARSHAQDTLWLCLEN 883

Query: 841  GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVIEEWTNEAVENEMDLILSAV 900
            GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSV EEWTNE+VE EMDLI+SAV
Sbjct: 884  GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWTNESVETEMDLIVSAV 943

Query: 901  KSLRSLAKESRERRPGYVLPRNDAVAEYINKRKLEIVTLANLSSLTVINDNDAAPIGCAV 960
            KSLRSLAKESRERRPGYVLPRND +AE INKRKLEIVTLANLSSLTVINDNDAAP+GCAV
Sbjct: 944  KSLRSLAKESRERRPGYVLPRNDVIAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAV 1003

Query: 961  SVVNENLSVYLQFQGAVSVEAELEKIKKKVEEIKKQQEKLKKMMDASGYKEKVRPHIHEE 1020
            SVVNENLSVYLQFQGA+S EAELEKIKKK+EE++KQQEKLKKMMDASGYKEKVRPHIHEE
Sbjct: 1004 SVVNENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEE 1063

Query: 1021 NVNKLASLMQELLSLEEAGQHIQAHR 1047
            NVNKLASLMQELLSLEEAGQHIQAHR
Sbjct: 1064 NVNKLASLMQELLSLEEAGQHIQAHR 1088

BLAST of HG10009470 vs. NCBI nr
Match: KAG7035615.1 (Valine--tRNA ligase, mitochondrial 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2045.0 bits (5297), Expect = 0.0e+00
Identity = 1007/1046 (96.27%), Postives = 1029/1046 (98.37%), Query Frame = 0

Query: 1    MESDKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAAPKKSEKKNARRG 60
            MES KKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSN APKKSEKKNARRG
Sbjct: 62   MESVKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSN-APKKSEKKNARRG 121

Query: 61   GDEENAEDFVDPDTPFGKKKLLSQQMAKQYNPSAVEKSWYEWWEKSEFFVADAKSSKPPF 120
            GDEENAEDFVDPDTPFG KKL+SQQM+KQYNPSAVEKSWYEWWEKS FFVADAKSSKPPF
Sbjct: 122  GDEENAEDFVDPDTPFGNKKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPF 181

Query: 121  VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM 180
            VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM
Sbjct: 182  VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM 241

Query: 181  RERNLTRHDIGREKFISEVWDWKTKYGGTILRQLRRLGASLDWTRECFTMDEKRSRAVTE 240
            RERNLTRHDIGREKFISEVWDWKTKYGGTIL+QLRRLGASLDWTRECFTMDEKRSRAVTE
Sbjct: 242  RERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTE 301

Query: 241  AFVRLFKNGVIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTS 300
            AFVRLFKNG+IYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYE PVEFGVLTS
Sbjct: 302  AFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTS 361

Query: 301  FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA 360
            FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA
Sbjct: 362  FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA 421

Query: 361  ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAR 420
            ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEF GMPRFK R
Sbjct: 422  ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKVR 481

Query: 421  EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDPAKQSLDAAMDNE 480
            EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKD AKQSLDA MDNE
Sbjct: 482  EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVMDNE 541

Query: 481  CKKLDIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWV 540
            CKK++IIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATL+DDQLKEFGAYNDHWV
Sbjct: 542  CKKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWV 601

Query: 541  VARNEEEAQEEARQIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTS 600
            VARNEEEA+EEA+ IYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPD+TEDLKAFYPTS
Sbjct: 602  VARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTS 661

Query: 601  ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI 660
            ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI
Sbjct: 662  ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI 721

Query: 661  NGISLEGLHKRLEEGNLDPKELAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN 720
            NGISLEGLHKRLEEGNLDPKELA+AKEGQVKDFPNGISECGADALRFALISYTAQSDKIN
Sbjct: 722  NGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN 781

Query: 721  LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPNKNVTSDVLPFSCQWILSVLNKAISRT 780
            LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVP+KNVT D LPFSCQWILSVLNKAISRT
Sbjct: 782  LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISRT 841

Query: 781  VSSLESYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEFASARSHAQDTLWLCLEN 840
            VSSLE+YEFSDATTAVYSWWQYQLCDVFIEAIKPYFS+NDTE+ASARSHAQDTLWLCLEN
Sbjct: 842  VSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSANDTEYASARSHAQDTLWLCLEN 901

Query: 841  GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVIEEWTNEAVENEMDLILSAV 900
            GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSV EEWTNE+VE EMDLI+SAV
Sbjct: 902  GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWTNESVETEMDLIVSAV 961

Query: 901  KSLRSLAKESRERRPGYVLPRNDAVAEYINKRKLEIVTLANLSSLTVINDNDAAPIGCAV 960
            KSLRSLAKESRERRPGYVLPRND VAE INKRKLEIVTLANLSSLTVINDNDAAP+GCAV
Sbjct: 962  KSLRSLAKESRERRPGYVLPRNDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAV 1021

Query: 961  SVVNENLSVYLQFQGAVSVEAELEKIKKKVEEIKKQQEKLKKMMDASGYKEKVRPHIHEE 1020
            SVVNENLSVYLQFQGA+S EAELEKIKKK+EE++KQQEKLKKMMDASGYKEKVRPHIHEE
Sbjct: 1022 SVVNENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEE 1081

Query: 1021 NVNKLASLMQELLSLEEAGQHIQAHR 1047
            NVNKLASLMQELLSLEEAGQHIQAHR
Sbjct: 1082 NVNKLASLMQELLSLEEAGQHIQAHR 1106

BLAST of HG10009470 vs. NCBI nr
Match: XP_022957954.1 (valine--tRNA ligase, mitochondrial 1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 2043.9 bits (5294), Expect = 0.0e+00
Identity = 1006/1046 (96.18%), Postives = 1029/1046 (98.37%), Query Frame = 0

Query: 1    MESDKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAAPKKSEKKNARRG 60
            MES KKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSN APKKSEKKNARRG
Sbjct: 62   MESVKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSN-APKKSEKKNARRG 121

Query: 61   GDEENAEDFVDPDTPFGKKKLLSQQMAKQYNPSAVEKSWYEWWEKSEFFVADAKSSKPPF 120
            GDEENAEDFVDPDTPFGKKKL+SQQM+KQYNPSAVEKSWYEWWEKS FFVADAKSSKPPF
Sbjct: 122  GDEENAEDFVDPDTPFGKKKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPF 181

Query: 121  VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM 180
            VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM
Sbjct: 182  VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM 241

Query: 181  RERNLTRHDIGREKFISEVWDWKTKYGGTILRQLRRLGASLDWTRECFTMDEKRSRAVTE 240
            RERNLTRHDIGREKFISEVWDWKTKYGGTIL+QLRRLGASLDWTRECFTMDEKRSRAVTE
Sbjct: 242  RERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTE 301

Query: 241  AFVRLFKNGVIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTS 300
            AFVRLFKNG+IYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYE PVEFGVLTS
Sbjct: 302  AFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTS 361

Query: 301  FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA 360
            FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA
Sbjct: 362  FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA 421

Query: 361  ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAR 420
            ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEF GMPRF+ R
Sbjct: 422  ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFEVR 481

Query: 421  EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDPAKQSLDAAMDNE 480
            EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKD AKQSLDA  DNE
Sbjct: 482  EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVTDNE 541

Query: 481  CKKLDIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWV 540
            CKK++IIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATL+DDQLKEFGAYNDHWV
Sbjct: 542  CKKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWV 601

Query: 541  VARNEEEAQEEARQIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTS 600
            VARNEEEA+EEA+ IYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPD+TEDLKAFYPTS
Sbjct: 602  VARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTS 661

Query: 601  ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI 660
            ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI
Sbjct: 662  ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI 721

Query: 661  NGISLEGLHKRLEEGNLDPKELAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN 720
            NGISLEGLHKRLEEGNLDPKELA+AKEGQVKDFPNGISECGADALRFALISYTAQSDKIN
Sbjct: 722  NGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN 781

Query: 721  LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPNKNVTSDVLPFSCQWILSVLNKAISRT 780
            LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVP+KNVT D LPFSCQWILSVLNKAISRT
Sbjct: 782  LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISRT 841

Query: 781  VSSLESYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEFASARSHAQDTLWLCLEN 840
            VSSLE+YEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTE+ASARSHAQDTLWLCLEN
Sbjct: 842  VSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEYASARSHAQDTLWLCLEN 901

Query: 841  GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVIEEWTNEAVENEMDLILSAV 900
            GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSV EEWTNE+VE EMDLI+SAV
Sbjct: 902  GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWTNESVETEMDLIVSAV 961

Query: 901  KSLRSLAKESRERRPGYVLPRNDAVAEYINKRKLEIVTLANLSSLTVINDNDAAPIGCAV 960
            KSLRSLAKESRERRPGYVLPRND VAE INKRKLEIVTLANLSSLTVINDNDAAP+GCAV
Sbjct: 962  KSLRSLAKESRERRPGYVLPRNDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAV 1021

Query: 961  SVVNENLSVYLQFQGAVSVEAELEKIKKKVEEIKKQQEKLKKMMDASGYKEKVRPHIHEE 1020
            SV+NENLSVYLQFQGA+S EAELEKIKKK+EE++KQQEKLKKMMDASGYKEKVRPHIHEE
Sbjct: 1022 SVINENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEE 1081

Query: 1021 NVNKLASLMQELLSLEEAGQHIQAHR 1047
            NVNKLASLMQELLSLEEAGQHIQAHR
Sbjct: 1082 NVNKLASLMQELLSLEEAGQHIQAHR 1106

BLAST of HG10009470 vs. ExPASy Swiss-Prot
Match: P93736 (Valine--tRNA ligase, mitochondrial 1 OS=Arabidopsis thaliana OX=3702 GN=TWN2 PE=1 SV=2)

HSP 1 Score: 1608.6 bits (4164), Expect = 0.0e+00
Identity = 774/1054 (73.43%), Postives = 897/1054 (85.10%), Query Frame = 0

Query: 2    ESDKKPEVEDP-EKKKKKEEKAREKELKKKKALEK----ALKLQAQQTSNAAPKKSEKKN 61
            ES+KK   E+  E+KKKKEEKA+EKELKK+KALEK     LK +  +     PKKS KK+
Sbjct: 47   ESEKKILTEEELERKKKKEEKAKEKELKKQKALEKERLAELKAKQAKDGTNVPKKSAKKS 106

Query: 62   ARRGGDEENAEDFVDPDTPFGKKKLLSQQMAKQYNPSAVEKSWYEWWEKSEFFVADAKSS 121
            ++R   EEN EDFVDP+TP G++K LS QMAKQY+P+ VEKSWY WWEKS+ F ADAKSS
Sbjct: 107  SKRDASEENPEDFVDPETPLGERKRLSSQMAKQYSPATVEKSWYAWWEKSDLFKADAKSS 166

Query: 122  KPPFVIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVE 181
            KPPFVIVLPPPNVTGALHIGHALT+AIED IIRW+RMSGYN LWVPG DHAGIATQVVVE
Sbjct: 167  KPPFVIVLPPPNVTGALHIGHALTSAIEDTIIRWKRMSGYNALWVPGVDHAGIATQVVVE 226

Query: 182  KKIMRERNLTRHDIGREKFISEVWDWKTKYGGTILRQLRRLGASLDWTRECFTMDEKRSR 241
            KKIMR+R +TRHD+GRE+F+ EVW WK +YGGTIL QLRRLGASLDW+RECFTMDE+RS+
Sbjct: 227  KKIMRDRGMTRHDVGREEFVKEVWKWKNQYGGTILTQLRRLGASLDWSRECFTMDEQRSK 286

Query: 242  AVTEAFVRLFKNGVIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFG 301
            AVTEAFVRL+K G+IYRD+RLVNWDC+LRTAISD+EV+YIDIKEKTLLKVPGYE PVEFG
Sbjct: 287  AVTEAFVRLYKEGLIYRDIRLVNWDCILRTAISDVEVEYIDIKEKTLLKVPGYEKPVEFG 346

Query: 302  VLTSFAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPI 361
            +LTSFAYPLEG LGE++VATTRVETMLGDTAIAIHPDD RYKHLHGKFA+HPFNGRKLPI
Sbjct: 347  LLTSFAYPLEGGLGEVIVATTRVETMLGDTAIAIHPDDARYKHLHGKFAVHPFNGRKLPI 406

Query: 362  ICDAILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPR 421
            ICD ILVDPNFGTG VKITPAHDPND EVGKRH LEFINIFTDDGKIN+NGGS+FAGMPR
Sbjct: 407  ICDGILVDPNFGTGCVKITPAHDPNDCEVGKRHKLEFINIFTDDGKINTNGGSDFAGMPR 466

Query: 422  FKAREMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDPAKQSLDAA 481
            F ARE VV+ALQK+GLYRGAK+NEMRLGLCSR+NDV+EPMIKPQWYVNC    K++LD A
Sbjct: 467  FAAREAVVEALQKQGLYRGAKNNEMRLGLCSRTNDVIEPMIKPQWYVNCSMIGKEALDVA 526

Query: 482  MDNECKKLDIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYN 541
            + +E KKL+ +PKQY+A+W+RWLENIRDWCISRQLWWGHRIPAWYATLE+DQLKE GAY+
Sbjct: 527  ITDENKKLEFVPKQYTAEWRRWLENIRDWCISRQLWWGHRIPAWYATLEEDQLKEVGAYS 586

Query: 542  DHWVVARNEEEAQEEARQIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAF 601
            DHWVVAR E++A+EEA Q + GKKF LT+DPDVLDTWFSSGLFPLSVLGWPD T+D KAF
Sbjct: 587  DHWVVARTEDDAREEAAQKFLGKKFELTRDPDVLDTWFSSGLFPLSVLGWPDVTDDFKAF 646

Query: 602  YPTSALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDP 661
            YPTS LETGHDILFFWVARMVM+G+ LGG+VPF  +Y HPMIRDAHGRKMSKSLGNVIDP
Sbjct: 647  YPTSVLETGHDILFFWVARMVMMGMKLGGEVPFSKVYFHPMIRDAHGRKMSKSLGNVIDP 706

Query: 662  VEVINGISLEGLHKRLEEGNLDPKELAIAKEGQVKDFPNGISECGADALRFALISYTAQS 721
            +EVING++LEGLHKRLEEGNLDPKE+ +AKEGQVKDFPNGI ECG DALRFAL+SYTAQS
Sbjct: 707  LEVINGVTLEGLHKRLEEGNLDPKEVIVAKEGQVKDFPNGIPECGTDALRFALVSYTAQS 766

Query: 722  DKINLDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPNKNVTSDVLPFSCQWILSVLNKA 781
            DKINLDI RVVGYRQWCNKLWNA+RFAM KLG+ Y P + ++ + +PFSCQWILSVLNKA
Sbjct: 767  DKINLDILRVVGYRQWCNKLWNAVRFAMMKLGDGYTPPQTLSPETMPFSCQWILSVLNKA 826

Query: 782  ISRTVSSLESYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEFASARSHAQDTLWL 841
            IS+TV SL+++EFSDA   +Y+WWQYQ CDV+IEAIKPYF+ ++  FAS R+HAQ  LW+
Sbjct: 827  ISKTVVSLDAFEFSDAANTIYAWWQYQFCDVYIEAIKPYFAGDNPTFASERAHAQHALWI 886

Query: 842  CLENGLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVIEEWTNEAVENEMDLI 901
             LE GLRLLHPFMP+VTEELWQRLP PK + R  SIMICDYPS IE W+NE VE+EMD +
Sbjct: 887  SLETGLRLLHPFMPFVTEELWQRLPAPKDTERKASIMICDYPSAIENWSNEKVESEMDTV 946

Query: 902  LSAVKSLRS-----LAKESRERRPGYVLPRNDAVAEYINKRKLEIVTLANLSSLTVINDN 961
            L+ VK +R+     L K+  ER P + L  N+  +E +   +LEI TLANLSSL V++  
Sbjct: 947  LATVKCMRALRAGLLEKQKNERLPAFALCENNVTSEIVKSHELEIRTLANLSSLEVVSKG 1006

Query: 962  D-AAPIGCAVSVVNENLSVYLQFQGAVSVEAELEKIKKKVEEIKKQQEKLKKMMDASGYK 1021
              AAP G +V  VNENL VYL+  GA++ EAE EKI+ K+ E++KQ+EKL+KMM  S Y+
Sbjct: 1007 QHAAPPGSSVETVNENLKVYLEVDGAINTEAEQEKIRNKIGELQKQKEKLQKMMSVSTYE 1066

Query: 1022 EKVRPHIHEENVNKLASLMQELLSLEEAGQHIQA 1045
            EKV  +I E+N NKLA ++QE    E+    + A
Sbjct: 1067 EKVPANIKEDNANKLAKILQEFDFFEKESARLAA 1100

BLAST of HG10009470 vs. ExPASy Swiss-Prot
Match: Q86KU2 (Probable valine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=valS1 PE=3 SV=1)

HSP 1 Score: 1027.3 bits (2655), Expect = 1.2e-298
Identity = 533/1049 (50.81%), Postives = 706/1049 (67.30%), Query Frame = 0

Query: 2    ESDKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAAPKKSEKKNARRGG 61
            E  K  E E  EK  K +EK  ++   K+K L+  LK + ++    A +K EK+ A    
Sbjct: 23   EKKKAKEAEKAEKLAKMKEKEAKQAAAKEKELQNKLKKEQEKKEKEAEEK-EKQEAEEKI 82

Query: 62   DEENAEDFVDPDTPFGKKKLLSQQMAKQYNPSAVEKSWYEWWEKSEFFVADAKSSKPP-- 121
              E+ E  +   TP G+KK +S  +   Y+P+AVE  WY++W  + +F  + +    P  
Sbjct: 83   VREHIEKIL--KTPKGEKKDVS-SLLSSYHPTAVESIWYDYWLDNGYFSPEKQMEIQPHV 142

Query: 122  -----FVIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVV 181
                 FVIV+PPPNVTG+LH+GHALT +I+DA++R+ RM G   LWVPGTDHAGIATQVV
Sbjct: 143  VKDKKFVIVIPPPNVTGSLHLGHALTNSIQDAVVRYHRMKGEVCLWVPGTDHAGIATQVV 202

Query: 182  VEKKIMRERNLTRHDIGREKFISEVWDWKTKYGGTILRQLRRLGASLDWTRECFTMDEKR 241
            VEKKI +E  +TRHD+GRE FI +VW+WK +YG  I  QL+++G+S+DW+RE FTMD++R
Sbjct: 203  VEKKIWKENKITRHDLGREGFIKKVWEWKGEYGARIQGQLKKMGSSVDWSREVFTMDDQR 262

Query: 242  SRAVTEAFVRLFKNGVIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVE 301
            S+AV  AF+R+F +G+I R  RLVNW C L+TAISDIEVD+ID+++ T + VPG++   +
Sbjct: 263  SKAVNTAFIRMFNDGLIVRTTRLVNWSCALKTAISDIEVDFIDLEKHTKMSVPGHQGQYD 322

Query: 302  FGVLTSFAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKL 361
            FGVL  FAY +EG   E+VVATTR+ETML DTAIAIHPDDTRY HLHGKFAIHP NGRK+
Sbjct: 323  FGVLFEFAYQVEGSGEELVVATTRIETMLADTAIAIHPDDTRYTHLHGKFAIHPLNGRKI 382

Query: 362  PIICDAILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGM 421
            PII D++LVD +FGTG VKITP+HDPND+E   RH LEFIN+FTD+G IN NGG  FAGM
Sbjct: 383  PIITDSVLVDKDFGTGVVKITPSHDPNDYETALRHGLEFINLFTDEGLINENGGERFAGM 442

Query: 422  PRFKAREMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDPAKQSLD 481
             RF AR  VV+AL++K L++G KDN+MRLGLCSRS DV+EPMIKPQWYV C D A +++ 
Sbjct: 443  KRFDARNAVVEALKEKNLFKGMKDNKMRLGLCSRSKDVIEPMIKPQWYVKCDDMAARAVK 502

Query: 482  AAMDNECKKLDIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGA 541
            A  D + K   I PK +   W RWLE I+DWC+SRQLWWGHRIPA++  ++  +   +  
Sbjct: 503  AVRDGDLK---ITPKTHEVTWYRWLEGIKDWCVSRQLWWGHRIPAYHCIIKGVKSNPYD- 562

Query: 542  YNDHWVVARNEEEAQEEARQIYAGKK----FHLTQDPDVLDTWFSSGLFPLSVLGWPDDT 601
              + WVV  N+EEA + A + +          L QDPDVLDTWFSSGLFP SV+GWP+ T
Sbjct: 563  -TNQWVVGANQEEATQNAIEKFKLSSKDDILSLEQDPDVLDTWFSSGLFPFSVMGWPEQT 622

Query: 602  EDLKAFYPTSALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSL 661
            +D++ +YPTS LETG DILFFWVARMVM+G  L G +PF  ++LH M+RD+HGRKMSKSL
Sbjct: 623  QDMQDYYPTSLLETGSDILFFWVARMVMMGQQLTGKLPFEQVFLHAMVRDSHGRKMSKSL 682

Query: 662  GNVIDPVEVINGISLEGLHKRLEEGNLDPKELAIAKEGQVKDFPNGISECGADALRFALI 721
            GNVIDP +VI GISL+ L  +L EGNLD KE+  A  G   DFP GI+ECG DA+RFAL 
Sbjct: 683  GNVIDPNDVIKGISLDELIAKLYEGNLDSKEIEKATSGVKSDFPTGIAECGTDAMRFALC 742

Query: 722  SYTAQSDKINLDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPNKNVTSDVLPFSCQ--- 781
            +YT+Q   INLDIQRVVGYR +CNK+WNA RFA  KLG+ + P     S +L  S     
Sbjct: 743  AYTSQGRDINLDIQRVVGYRNFCNKIWNATRFAHMKLGDSFKPESFDASKLLQQSNAINL 802

Query: 782  WILSVLNKAISRTVSSLESYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEFASAR 841
            WIL+   +AI+ +    + Y+FS  TTA+YS+W  ++CDV++E  K  FS    E +  +
Sbjct: 803  WILNAAQRAITLSEEGFKEYDFSKVTTAIYSFWLNEMCDVYLEMTKSIFSLE--EDSPIK 862

Query: 842  SHAQDTLWLCLENGLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVIEEWTNE 901
               ++TL+ C++ GLRLLHPFMPY+TEEL+Q LP       P +IM+C YP     W N 
Sbjct: 863  QKTKETLYTCIDIGLRLLHPFMPYLTEELYQSLPRRPEDVIP-TIMLCPYPQSQSCWANS 922

Query: 902  AVENEMDLILSAVKSLRSLAK----ESRERRPGYVLPRNDAVAEYINKRKLEIVTLANLS 961
             +E EM      +KS+RSL       + ++   Y+  +N    E   K    I  LA  S
Sbjct: 923  DIEEEMKQCQDIIKSIRSLRATYTIPASKKLHTYLHVKNQENLERFTKNSSFIRVLAYAS 982

Query: 962  SLTVINDNDAAPIGCAVSVVNENLSVYLQFQGAVSVEAELEKIKKKVEEIKKQQEKLKKM 1021
             L V   +++ P GC V+VVNEN+S  L  +G+V    E+ +++ K +++ K  E L   
Sbjct: 983  ELEVSISDESRP-GCIVNVVNENVSTLLDVRGSVDFNLEIARLETKKQQLVKNYETLVSK 1042

Query: 1022 MDASGYKEKVRPHIHEENVNKLASLMQEL 1033
                 Y +KV  +I ++N  K+ +L +E+
Sbjct: 1043 TTIPSY-DKVPQNIRDDNTQKIKALDEEI 1057

BLAST of HG10009470 vs. ExPASy Swiss-Prot
Match: P49696 (Valine--tRNA ligase OS=Takifugu rubripes OX=31033 GN=vars1 PE=3 SV=1)

HSP 1 Score: 978.0 bits (2527), Expect = 8.3e-284
Identity = 511/1050 (48.67%), Postives = 692/1050 (65.90%), Query Frame = 0

Query: 13   EKKKKKEEKAREK--ELKKKKALEKALKLQAQQTSNAAPKKSEKKNARRGGDEENAEDFV 72
            E + KKE K REK  + ++KK +E   K+Q      A P+K            E      
Sbjct: 181  EAQLKKEAKKREKLEKFQQKKEMEAKKKMQPVAEKKAKPEK-----------RELGVITY 240

Query: 73   DPDTPFGKKKLLSQQMAKQYNPSAVEKSWYEWWEKSEFFVAD-------AKSSKPPFVIV 132
            D  TP G+KK +   +   Y+P  VE +WY WWEK  FF  +        ++ +  F++ 
Sbjct: 241  DIPTPSGEKKDVVSPLPDSYSPQYVEAAWYPWWEKQGFFKPEFGRKSIGEQNPRGIFMMC 300

Query: 133  LPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRER 192
            +PPPNVTG+LH+GHALT AI+D + RW RM G  TLW PG DHAGIATQVVVEKK+MRE+
Sbjct: 301  IPPPNVTGSLHLGHALTNAIQDTLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLMREK 360

Query: 193  NLTRHDIGREKFISEVWDWKTKYGGTILRQLRRLGASLDWTRECFTMDEKRSRAVTEAFV 252
              +RHD+GREKFI EVW WK + G  I  QL++LG+SLDW R CFTMD K S AV EAF+
Sbjct: 361  GTSRHDLGREKFIEEVWKWKNEKGDRIYHQLKKLGSSLDWDRACFTMDPKLSYAVQEAFI 420

Query: 253  RLFKNGVIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAY 312
            R+   GVIYR  RLVNW C L +AISDIEVD  ++  +TLL VPGY+  VEFGVL SFAY
Sbjct: 421  RMHDEGVIYRSKRLVNWSCSLNSAISDIEVDKNELSGRTLLPVPGYKEKVEFGVLVSFAY 480

Query: 313  PLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDAILV 372
             ++G   E+VVATTR+ETMLGDTA+A+HP D+RY+HL GK  +HPF  RK+P++ D   V
Sbjct: 481  KVDGSDEEVVVATTRIETMLGDTAVAVHPSDSRYQHLKGKTVLHPFCDRKIPVVFDD-FV 540

Query: 373  DPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAREMV 432
            D +FGTGAVKITPAHD ND+EVG RHNL FINI  ++G +  N    F GM RF AR+ V
Sbjct: 541  DMSFGTGAVKITPAHDHNDYEVGVRHNLAFINILDENGFV-INVPPPFLGMKRFDARKAV 600

Query: 433  VDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDPAKQSLDAAMDNECKK 492
            + AL+ +  ++  KDN M + +CSRS D+VEP++KPQWYV+C D  KQ+ DA  +    +
Sbjct: 601  LQALKDRDQFKEIKDNPMVVPVCSRSKDIVEPLMKPQWYVSCSDMGKQAADAVREG---R 660

Query: 493  LDIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHW-VVA 552
            L IIP  +S  W  W++NIRDWCISRQLWWGHRIPA++ T+ D  +K     + H+ V  
Sbjct: 661  LKIIPDHHSQTWFNWMDNIRDWCISRQLWWGHRIPAYFITVSDPSVKPGEDMDGHYRVSG 720

Query: 553  RNEEEAQEEARQIY--AGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTS 612
            R  EEA+E+A + +  +  K  L QD DVLDTWFSSG+ P S+LGWP++TEDL  FYP +
Sbjct: 721  RTPEEAREKAAKRFNVSPDKIALRQDEDVLDTWFSSGINPFSILGWPNETEDLNVFYPGT 780

Query: 613  ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI 672
             LETGHDILFFWVARMVM+G+ L G +PF+ +Y   ++RDAHGRKMSKSLGNVIDP++  
Sbjct: 781  LLETGHDILFFWVARMVMMGLKLTGKLPFKEVYHCAVVRDAHGRKMSKSLGNVIDPLDDH 840

Query: 673  NGISLEGLHKRLEEGNLDPKELAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN 732
             GI+LEGLH +L + NLDP E+   K+ Q  D+PN I ECG DALRFAL +YT+Q   IN
Sbjct: 841  IGIALEGLHAQLMDTNLDPLEVEKPKKVQKADYPNCIPECGTDALRFALCAYTSQGRDIN 900

Query: 733  LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVP-NKNVTSDVLPFSCQWILSVLNKAISR 792
            LD+ R++GYR +CNKLWNA++FAM  LG+ +VP + +        S +WILS L+ A+++
Sbjct: 901  LDVNRILGYRHFCNKLWNAVKFAMRTLGDQFVPADTSPAEREESVSDRWILSRLSTAVAQ 960

Query: 793  TVSSLESYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEFASARSHA--QDTLWLC 852
              ++  +Y+F   TTA+Y++W Y+LCDV++E++KP F     + +  R  A  + TL+ C
Sbjct: 961  CDAAFRTYDFPAITTAIYNFWLYELCDVYLESVKPVFIKAKEDGSCERPAAVCRQTLYTC 1020

Query: 853  LENGLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVIEE-WTNEAVENEMDLI 912
            LE GLRLL P MP+VTEEL+QRLP  +  + P SI +  YP   E  W  E V+ ++D I
Sbjct: 1021 LEVGLRLLAPLMPFVTEELYQRLPRRRPQSDPPSICVTPYPDAAEFCWQCEDVDRDIDFI 1080

Query: 913  LSAVKSLRSLAKESRERRPG---YVLPRNDAVAEYINKRKLEIVTLANLSSLTVINDNDA 972
            +  V+++RSL  + +  +     Y+   + A    + K  L+I TL+   ++  +     
Sbjct: 1081 MGVVRTIRSLRSDYKLTKTAADCYLQCTDAATVSLVQKYSLQIQTLSYSQAIVPLMAPQP 1140

Query: 973  APIGCAVSVVNENLSVYLQFQGAVSVEAELEKIKKKVEEIKKQQEKLKKMMDASGYKEKV 1032
            AP GCAV++ ++  +V +  +G + VE E+ K+  K  +++KQ EKL + +    YKEKV
Sbjct: 1141 APEGCAVAIASDRCTVNMMLKGLIDVEKEVPKLMGKKTDLEKQIEKLSEKISKGDYKEKV 1200

Query: 1033 RPHIHEENVNKLASLMQELLSLEEAGQHIQ 1044
               + E++  KL     EL  ++EA  + Q
Sbjct: 1201 PVKVQEQDTEKLRQSQTELEKVKEAMDNFQ 1214

BLAST of HG10009470 vs. ExPASy Swiss-Prot
Match: P26640 (Valine--tRNA ligase OS=Homo sapiens OX=9606 GN=VARS1 PE=1 SV=4)

HSP 1 Score: 966.8 bits (2498), Expect = 1.9e-280
Identity = 512/1033 (49.56%), Postives = 681/1033 (65.92%), Query Frame = 0

Query: 24   EKELKKKKALEKALKLQAQQTSNAAPKKSEKKNARRGGDEENAEDFVDPDTPFGKKKLLS 83
            +KE KK++ LEK  + Q  Q     P + + K  +R   +     + D  TP G+KK +S
Sbjct: 235  KKEAKKREKLEKFQQKQKIQQQQPPPGEKKPKPEKREKRDPGVITY-DLPTPPGEKKDVS 294

Query: 84   QQMAKQYNPSAVEKSWYEWWEKSEFFVAD-------AKSSKPPFVIVLPPPNVTGALHIG 143
              M   Y+P  VE +WY WWE+  FF  +       A + +  F++ +PPPNVTG+LH+G
Sbjct: 295  GPMPDSYSPRYVEAAWYPWWEQQGFFKPEYGRPNVSAANPRGVFMMCIPPPNVTGSLHLG 354

Query: 144  HALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFI 203
            HALT AI+D++ RW RM G  TLW PG DHAGIATQVVVEKK+ RE+ L+RH +GRE F+
Sbjct: 355  HALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWREQGLSRHQLGREAFL 414

Query: 204  SEVWDWKTKYGGTILRQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGVIYRDLR 263
             EVW WK + G  I  QL++LG+SLDW R CFTMD K S AVTEAFVRL + G+IYR  R
Sbjct: 415  QEVWKWKEEKGDRIYHQLKKLGSSLDWDRACFTMDPKLSAAVTEAFVRLHEEGIIYRSTR 474

Query: 264  LVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELG--EIVV 323
            LVNW C L +AISDIEVD  ++  +TLL VPGY+  VEFGVL SFAY ++G     E+VV
Sbjct: 475  LVNWSCTLNSAISDIEVDKKELTGRTLLSVPGYKEKVEFGVLVSFAYKVQGSDSDEEVVV 534

Query: 324  ATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDAILVDPNFGTGAVKI 383
            ATTR+ETMLGD A+A+HP DTRY+HL GK  IHPF  R LPI+ D   VD +FGTGAVKI
Sbjct: 535  ATTRIETMLGDVAVAVHPKDTRYQHLKGKNVIHPFLSRSLPIVFDE-FVDMDFGTGAVKI 594

Query: 384  TPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAREMVVDALQKKGLYR 443
            TPAHD ND+EVG+RH LE I+I    G +  N    F G+PRF+AR+ V+ AL+++GL+R
Sbjct: 595  TPAHDQNDYEVGQRHGLEAISIMDSRGAL-INVPPPFLGLPRFEARKAVLVALKERGLFR 654

Query: 444  GAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDPAKQSLDAAMDNECKKLDIIPKQYSAD 503
            G +DN M + LC+RS DVVEP+++PQWYV C + A+ +  A    +   L I+P+ +   
Sbjct: 655  GIEDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGD---LRILPEAHQRT 714

Query: 504  WKRWLENIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYND--HWVVARNEEEAQEEA 563
            W  W++NIR+WCISRQLWWGHRIPA++ T+ D  +   G   D  +WV  RNE EA+E+A
Sbjct: 715  WHAWMDNIREWCISRQLWWGHRIPAYFVTVSDPAVPP-GEDPDGRYWVSGRNEAEAREKA 774

Query: 564  RQIY--AGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTSALETGHDILF 623
             + +  +  K  L QD DVLDTWFSSGLFPLS+LGWP+ +EDL  FYP + LETGHDILF
Sbjct: 775  AKEFGVSPDKISLQQDEDVLDTWFSSGLFPLSILGWPNQSEDLSVFYPGTLLETGHDILF 834

Query: 624  FWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHK 683
            FWVARMVMLG+ L G +PFR +YLH ++RDAHGRKMSKSLGNVIDP++VI GISL+GLH 
Sbjct: 835  FWVARMVMLGLKLTGRLPFREVYLHAIVRDAHGRKMSKSLGNVIDPLDVIYGISLQGLHN 894

Query: 684  RLEEGNLDPKELAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYR 743
            +L   NLDP E+  AKEGQ  DFP GI ECG DALRF L +Y +Q   INLD+ R++GYR
Sbjct: 895  QLLNSNLDPSEVEKAKEGQKADFPAGIPECGTDALRFGLCAYMSQGRDINLDVNRILGYR 954

Query: 744  QWCNKLWNAIRFAMSKLGNDYVPN-KNVTSDVLPFSCQWILSVLNKAISRTVSSLESYEF 803
             +CNKLWNA +FA+  LG  +VP+  +          +WI S L +A+  +    ++Y+F
Sbjct: 955  HFCNKLWNATKFALRGLGKGFVPSPTSQPGGHESLVDRWIRSRLTEAVRLSNQGFQAYDF 1014

Query: 804  SDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEFASARSHAQDTLWLCLENGLRLLHPFM 863
               TTA YS+W Y+LCDV++E +KP  +  D   A     A+ TL+ CL+ GLRLL PFM
Sbjct: 1015 PAVTTAQYSFWLYELCDVYLECLKPVLNGVDQVAAEC---ARQTLYTCLDVGLRLLSPFM 1074

Query: 864  PYVTEELWQRLPYPKSSTRPESIMICDYPSVIE-EWTNEAVENEMDLILSAVKSLRSLAK 923
            P+VTEEL+QRLP  +    P S+ +  YP   E  W +   E  ++L LS  +++RSL  
Sbjct: 1075 PFVTEELFQRLP-RRMPQAPPSLCVTPYPEPSECSWKDPEAEAALELALSITRAVRSLRA 1134

Query: 924  E---SRERRPGYVLPRNDAVAEYINKRKLEIVTLANLSSLTVINDNDAAPIGCAVSVVNE 983
            +   +R R   ++   ++A     +     +  LA+   + V+     AP GCAV++ ++
Sbjct: 1135 DYNLTRIRPDCFLEVADEATGALASAVSGYVQALASAGVVAVLALGAPAPQGCAVALASD 1194

Query: 984  NLSVYLQFQGAVSVEAELEKIKKKVEEIKKQQEKLKKMMDASGYKEKVRPHIHEENVNKL 1039
              S++LQ QG V    EL K++ K  E ++Q ++L++   ASGY  KV   + E +  KL
Sbjct: 1195 RCSIHLQLQGLVDPARELGKLQAKRVEAQRQAQRLRERRAASGYPVKVPLEVQEADEAKL 1254

BLAST of HG10009470 vs. ExPASy Swiss-Prot
Match: Q9Z1Q9 (Valine--tRNA ligase OS=Mus musculus OX=10090 GN=Vars1 PE=1 SV=1)

HSP 1 Score: 964.5 bits (2492), Expect = 9.5e-280
Identity = 508/1033 (49.18%), Postives = 674/1033 (65.25%), Query Frame = 0

Query: 24   EKELKKKKALEKALKLQAQQTSNAAPKKSEKKNARRGGDEENAEDFVDPDTPFGKKKLLS 83
            +KE KK++ LEK    Q Q+T    P   +K    +    +      D  TP G+KK +S
Sbjct: 235  KKEAKKREKLEKF--QQKQKTQQQPPHGEKKPKPEKKEKRDPGVITYDLPTPPGEKKDVS 294

Query: 84   QQMAKQYNPSAVEKSWYEWWEKSEFFVAD-------AKSSKPPFVIVLPPPNVTGALHIG 143
              M   Y+P  VE +WY WWE+  FF  +       A + +  F++ +PPPNVTG+LH+G
Sbjct: 295  GAMPDSYSPQYVEAAWYPWWERQGFFKPEYGRPSVSAPNPRGVFMMCIPPPNVTGSLHLG 354

Query: 144  HALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFI 203
            HALT AI+D++ RW RM G  TLW PG DHAGIATQVVVEKK+ +ER L RH +GRE F+
Sbjct: 355  HALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWKERGLNRHQLGREAFL 414

Query: 204  SEVWDWKTKYGGTILRQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGVIYRDLR 263
             EVW WK + G  I  QL++LG+SLDW R CFTMD K S  VTEAFVRL + GVIYR  R
Sbjct: 415  EEVWKWKAEKGDRIYHQLKKLGSSLDWDRACFTMDPKLSATVTEAFVRLHEEGVIYRSTR 474

Query: 264  LVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELG--EIVV 323
            LVNW C L +AISDIEVD  ++  +TLL VPGY+  VEFGVL SFAY ++G     E+VV
Sbjct: 475  LVNWSCTLNSAISDIEVDKKELTGRTLLPVPGYKEKVEFGVLVSFAYKVQGSDSDEEVVV 534

Query: 324  ATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDAILVDPNFGTGAVKI 383
            ATTR+ETMLGD A+A+HP D RY+HL GK  +HPF  R LPI+ D   VD  FGTGAVKI
Sbjct: 535  ATTRIETMLGDVAVAVHPKDPRYQHLKGKCVVHPFLSRSLPIVFDD-FVDMEFGTGAVKI 594

Query: 384  TPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAREMVVDALQKKGLYR 443
            TPAHD ND+EVG+RH LE I+I    G +  N    F G+PRF+AR+ V+ AL+++GL+R
Sbjct: 595  TPAHDQNDYEVGQRHRLEAISIMDSKGAL-INVPPPFLGLPRFEARKAVLAALKERGLFR 654

Query: 444  GAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDPAKQSLDAAMDNECKKLDIIPKQYSAD 503
            G KDN M + LC+RS DVVEP+++PQWYV C + A+ +  A    +   L I+P+ +   
Sbjct: 655  GVKDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGD---LRILPEAHQRT 714

Query: 504  WKRWLENIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYND--HWVVARNEEEAQEEA 563
            W  W++NIRDWCISRQLWWGHRIPA++ T+ D  +   G   D  +WV  R E EA+E+A
Sbjct: 715  WHSWMDNIRDWCISRQLWWGHRIPAYFITVHDPAVPP-GEDPDGRYWVSGRTEAEAREKA 774

Query: 564  RQIY--AGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTSALETGHDILF 623
             + +  +  K  L QD DVLDTWFSSGLFP S+ GWP+ +EDL  FYP + LETGHDILF
Sbjct: 775  AREFGVSPDKISLQQDEDVLDTWFSSGLFPFSIFGWPNQSEDLSVFYPGTLLETGHDILF 834

Query: 624  FWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHK 683
            FWVARMVMLG+ L G +PFR +YLH ++RDAHGRKMSKSLGNVIDP++VI+G+SL+GL+ 
Sbjct: 835  FWVARMVMLGLKLTGKLPFREVYLHAIVRDAHGRKMSKSLGNVIDPLDVIHGVSLQGLYD 894

Query: 684  RLEEGNLDPKELAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYR 743
            +L   NLDP E+  AKEGQ  DFP GI ECG DALRF L +YT+Q   INLD+ R++GYR
Sbjct: 895  QLLNSNLDPSEVEKAKEGQKADFPAGIPECGTDALRFGLCAYTSQGRDINLDVNRILGYR 954

Query: 744  QWCNKLWNAIRFAMSKLGNDYVPNKNVTSD-VLPFSCQWILSVLNKAISRTVSSLESYEF 803
             +CNKLWNA +FA+  LG  +VP+     +       +WI S L +A+  +    ++Y+F
Sbjct: 955  HFCNKLWNATKFALRGLGKGFVPSATSKPEGHESLVDRWIRSRLTEAVRLSNEGFQAYDF 1014

Query: 804  SDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEFASARSHAQDTLWLCLENGLRLLHPFM 863
               TTA YS+W Y+LCDV++E +KP  +  D   A     A+ TL+ CL+ GLRLL PFM
Sbjct: 1015 PAITTAQYSFWLYELCDVYLECLKPVLNGVDQVAAEC---ARQTLYTCLDVGLRLLSPFM 1074

Query: 864  PYVTEELWQRLPYPKSSTRPESIMICDYPSVIE-EWTNEAVENEMDLILSAVKSLRSLAK 923
            P+VTEEL+QRLP  ++   P S+ +  YP   E  W +   E  ++L LS  +++RSL  
Sbjct: 1075 PFVTEELFQRLP-RRTPKAPASLCVTPYPEPSECSWKDPEAEAALELALSITRAVRSLRA 1134

Query: 924  E---SRERRPGYVLPRNDAVAEYINKRKLEIVTLANLSSLTVINDNDAAPIGCAVSVVNE 983
            +   +R R   ++   ++A     +     +  LA+   + V+     AP GCAV+V ++
Sbjct: 1135 DYNLTRTRPDCFLEVADEATGALASAVSGYVQALASAGVVAVLALGAPAPQGCAVAVASD 1194

Query: 984  NLSVYLQFQGAVSVEAELEKIKKKVEEIKKQQEKLKKMMDASGYKEKVRPHIHEENVNKL 1039
              S++LQ QG V    EL K++ K  E ++Q ++L++   AS Y  KV   + E +  KL
Sbjct: 1195 RCSIHLQLQGLVDPARELGKLQAKRSEAQRQAQRLQERRAASSYSAKVPLEVQEADEAKL 1254

BLAST of HG10009470 vs. ExPASy TrEMBL
Match: A0A1S3CT04 (Valyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103504618 PE=3 SV=1)

HSP 1 Score: 2046.9 bits (5302), Expect = 0.0e+00
Identity = 1005/1046 (96.08%), Postives = 1034/1046 (98.85%), Query Frame = 0

Query: 1    MESDKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAAPKKSEKKNARRG 60
            ME DKKPE+EDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSN APKKSEKKNARRG
Sbjct: 1    MEPDKKPEMEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSN-APKKSEKKNARRG 60

Query: 61   GDEENAEDFVDPDTPFGKKKLLSQQMAKQYNPSAVEKSWYEWWEKSEFFVADAKSSKPPF 120
            GDEENAEDFVDPDTPFGKKKLLSQQMAKQYNPSAVEKSWYEWWEKS +FVADAKSSKPPF
Sbjct: 61   GDEENAEDFVDPDTPFGKKKLLSQQMAKQYNPSAVEKSWYEWWEKSGYFVADAKSSKPPF 120

Query: 121  VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM 180
            VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSG+NTLWVPGTDHAGIATQVVVEKKIM
Sbjct: 121  VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGFNTLWVPGTDHAGIATQVVVEKKIM 180

Query: 181  RERNLTRHDIGREKFISEVWDWKTKYGGTILRQLRRLGASLDWTRECFTMDEKRSRAVTE 240
            RERNLTRHDIGREKFISEVW+WKTKYGGTIL+QLRRLGASLDWTRECFTMDEKRSRAVTE
Sbjct: 181  RERNLTRHDIGREKFISEVWEWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTE 240

Query: 241  AFVRLFKNGVIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTS 300
            AFVRLFKNG+IYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLL+VPGYENPVEFGVLTS
Sbjct: 241  AFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLRVPGYENPVEFGVLTS 300

Query: 301  FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA 360
            FAYPLEGELGEIVVATTRVETMLGDTAIA+HP+DTRYKHLHGKFAIHPFNGRKLPIICDA
Sbjct: 301  FAYPLEGELGEIVVATTRVETMLGDTAIAVHPEDTRYKHLHGKFAIHPFNGRKLPIICDA 360

Query: 361  ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAR 420
            ILVDP FGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAR
Sbjct: 361  ILVDPKFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAR 420

Query: 421  EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDPAKQSLDAAMDNE 480
            EMVVDALQKKGLYRGAKDNEMRLGLCSR+NDVVEPMIKPQWYVNCK+PAKQSLDAAMDNE
Sbjct: 421  EMVVDALQKKGLYRGAKDNEMRLGLCSRTNDVVEPMIKPQWYVNCKEPAKQSLDAAMDNE 480

Query: 481  CKKLDIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWV 540
            CKK+DIIPKQYSADWKRWL+NIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWV
Sbjct: 481  CKKIDIIPKQYSADWKRWLDNIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWV 540

Query: 541  VARNEEEAQEEARQIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTS 600
            VARNEEEA+EEA++IYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTS
Sbjct: 541  VARNEEEAEEEAKRIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTS 600

Query: 601  ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI 660
            ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI
Sbjct: 601  ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI 660

Query: 661  NGISLEGLHKRLEEGNLDPKELAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN 720
            NGISLEGLHKRLEEGNLDPKELAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN
Sbjct: 661  NGISLEGLHKRLEEGNLDPKELAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN 720

Query: 721  LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPNKNVTSDVLPFSCQWILSVLNKAISRT 780
            LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPNKN T DVLPFSCQWILSVLNKAISRT
Sbjct: 721  LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPNKNATPDVLPFSCQWILSVLNKAISRT 780

Query: 781  VSSLESYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEFASARSHAQDTLWLCLEN 840
            +SSLESYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSN+T++ASARSHAQDTLWLCLEN
Sbjct: 781  ISSLESYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNETDYASARSHAQDTLWLCLEN 840

Query: 841  GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVIEEWTNEAVENEMDLILSAV 900
            GLRLLHPFMPYVTEELWQRLPYPK+STRPESIMICDYPSV EEWTNEAVENEMDLI+SAV
Sbjct: 841  GLRLLHPFMPYVTEELWQRLPYPKNSTRPESIMICDYPSVTEEWTNEAVENEMDLIVSAV 900

Query: 901  KSLRSLAKESRERRPGYVLPRNDAVAEYINKRKLEIVTLANLSSLTVINDNDAAPIGCAV 960
            +SLRSLAKESRER+PGYVLPRN AVAE INKRKLEIVTLANLSSLTVINDNDAAP+GCAV
Sbjct: 901  RSLRSLAKESRERKPGYVLPRNLAVAEIINKRKLEIVTLANLSSLTVINDNDAAPVGCAV 960

Query: 961  SVVNENLSVYLQFQGAVSVEAELEKIKKKVEEIKKQQEKLKKMMDASGYKEKVRPHIHEE 1020
            SVVNENLSVYLQFQGA+S EAELEKI KK++EIKKQQE+LKKMMDASGYKEKVRP IHEE
Sbjct: 961  SVVNENLSVYLQFQGAISAEAELEKINKKIDEIKKQQERLKKMMDASGYKEKVRPQIHEE 1020

Query: 1021 NVNKLASLMQELLSLEEAGQHIQAHR 1047
            NVNKLASLMQELLSLEEAGQHIQAHR
Sbjct: 1021 NVNKLASLMQELLSLEEAGQHIQAHR 1045

BLAST of HG10009470 vs. ExPASy TrEMBL
Match: A0A6J1H3K8 (Valyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111459332 PE=3 SV=1)

HSP 1 Score: 2043.9 bits (5294), Expect = 0.0e+00
Identity = 1006/1046 (96.18%), Postives = 1029/1046 (98.37%), Query Frame = 0

Query: 1    MESDKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAAPKKSEKKNARRG 60
            MES KKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSN APKKSEKKNARRG
Sbjct: 62   MESVKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSN-APKKSEKKNARRG 121

Query: 61   GDEENAEDFVDPDTPFGKKKLLSQQMAKQYNPSAVEKSWYEWWEKSEFFVADAKSSKPPF 120
            GDEENAEDFVDPDTPFGKKKL+SQQM+KQYNPSAVEKSWYEWWEKS FFVADAKSSKPPF
Sbjct: 122  GDEENAEDFVDPDTPFGKKKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPF 181

Query: 121  VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM 180
            VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM
Sbjct: 182  VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM 241

Query: 181  RERNLTRHDIGREKFISEVWDWKTKYGGTILRQLRRLGASLDWTRECFTMDEKRSRAVTE 240
            RERNLTRHDIGREKFISEVWDWKTKYGGTIL+QLRRLGASLDWTRECFTMDEKRSRAVTE
Sbjct: 242  RERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTE 301

Query: 241  AFVRLFKNGVIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTS 300
            AFVRLFKNG+IYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYE PVEFGVLTS
Sbjct: 302  AFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTS 361

Query: 301  FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA 360
            FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA
Sbjct: 362  FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA 421

Query: 361  ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAR 420
            ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEF GMPRF+ R
Sbjct: 422  ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFEVR 481

Query: 421  EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDPAKQSLDAAMDNE 480
            EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKD AKQSLDA  DNE
Sbjct: 482  EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVTDNE 541

Query: 481  CKKLDIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWV 540
            CKK++IIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATL+DDQLKEFGAYNDHWV
Sbjct: 542  CKKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWV 601

Query: 541  VARNEEEAQEEARQIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTS 600
            VARNEEEA+EEA+ IYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPD+TEDLKAFYPTS
Sbjct: 602  VARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTS 661

Query: 601  ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI 660
            ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI
Sbjct: 662  ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI 721

Query: 661  NGISLEGLHKRLEEGNLDPKELAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN 720
            NGISLEGLHKRLEEGNLDPKELA+AKEGQVKDFPNGISECGADALRFALISYTAQSDKIN
Sbjct: 722  NGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN 781

Query: 721  LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPNKNVTSDVLPFSCQWILSVLNKAISRT 780
            LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVP+KNVT D LPFSCQWILSVLNKAISRT
Sbjct: 782  LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISRT 841

Query: 781  VSSLESYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEFASARSHAQDTLWLCLEN 840
            VSSLE+YEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTE+ASARSHAQDTLWLCLEN
Sbjct: 842  VSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEYASARSHAQDTLWLCLEN 901

Query: 841  GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVIEEWTNEAVENEMDLILSAV 900
            GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSV EEWTNE+VE EMDLI+SAV
Sbjct: 902  GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWTNESVETEMDLIVSAV 961

Query: 901  KSLRSLAKESRERRPGYVLPRNDAVAEYINKRKLEIVTLANLSSLTVINDNDAAPIGCAV 960
            KSLRSLAKESRERRPGYVLPRND VAE INKRKLEIVTLANLSSLTVINDNDAAP+GCAV
Sbjct: 962  KSLRSLAKESRERRPGYVLPRNDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAV 1021

Query: 961  SVVNENLSVYLQFQGAVSVEAELEKIKKKVEEIKKQQEKLKKMMDASGYKEKVRPHIHEE 1020
            SV+NENLSVYLQFQGA+S EAELEKIKKK+EE++KQQEKLKKMMDASGYKEKVRPHIHEE
Sbjct: 1022 SVINENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEE 1081

Query: 1021 NVNKLASLMQELLSLEEAGQHIQAHR 1047
            NVNKLASLMQELLSLEEAGQHIQAHR
Sbjct: 1082 NVNKLASLMQELLSLEEAGQHIQAHR 1106

BLAST of HG10009470 vs. ExPASy TrEMBL
Match: A0A6J1JYM1 (Valyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111490926 PE=3 SV=1)

HSP 1 Score: 2039.2 bits (5282), Expect = 0.0e+00
Identity = 1005/1046 (96.08%), Postives = 1027/1046 (98.18%), Query Frame = 0

Query: 1    MESDKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAAPKKSEKKNARRG 60
            MES KKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSN APKKSEKKNARRG
Sbjct: 63   MESVKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSN-APKKSEKKNARRG 122

Query: 61   GDEENAEDFVDPDTPFGKKKLLSQQMAKQYNPSAVEKSWYEWWEKSEFFVADAKSSKPPF 120
            GDEENAEDFVDPDTPFGKKKL+SQQM+KQYNPSAVEKSWYEWWEKS FFVADAKSSKPPF
Sbjct: 123  GDEENAEDFVDPDTPFGKKKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPF 182

Query: 121  VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM 180
            VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM
Sbjct: 183  VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM 242

Query: 181  RERNLTRHDIGREKFISEVWDWKTKYGGTILRQLRRLGASLDWTRECFTMDEKRSRAVTE 240
            RERNLTRHDIGREKFISEVWDWKTKYGGTIL+QLRRLGASLDWTRECFTMDEKRSRAVTE
Sbjct: 243  RERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTE 302

Query: 241  AFVRLFKNGVIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTS 300
            AFVRLFKNG+IYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYE PVEFGVLTS
Sbjct: 303  AFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTS 362

Query: 301  FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA 360
            FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA
Sbjct: 363  FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA 422

Query: 361  ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAR 420
            ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEF GMPRFK R
Sbjct: 423  ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKVR 482

Query: 421  EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDPAKQSLDAAMDNE 480
            EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKD AKQSLDA  DNE
Sbjct: 483  EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVTDNE 542

Query: 481  CKKLDIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWV 540
            CKK+ IIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATL+DDQLKEFGAYNDHWV
Sbjct: 543  CKKIKIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWV 602

Query: 541  VARNEEEAQEEARQIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTS 600
            VARNEEEA+EEA+ IYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPD+TEDLKAFYPTS
Sbjct: 603  VARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTS 662

Query: 601  ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI 660
            ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI
Sbjct: 663  ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI 722

Query: 661  NGISLEGLHKRLEEGNLDPKELAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN 720
            NGISLEGLHKRLEEGNLDPKELA+AKEGQVKDFPNGISECGADALRFALISYTAQSDKIN
Sbjct: 723  NGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN 782

Query: 721  LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPNKNVTSDVLPFSCQWILSVLNKAISRT 780
            LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVP+KNVT D LPFSCQWILSVLNKAISRT
Sbjct: 783  LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISRT 842

Query: 781  VSSLESYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEFASARSHAQDTLWLCLEN 840
            VSSLE+YEFSDATTAVY WWQYQLCDVFIEAIKPYFSSNDTE+ASARSHAQDTLWLCLEN
Sbjct: 843  VSSLEAYEFSDATTAVYYWWQYQLCDVFIEAIKPYFSSNDTEYASARSHAQDTLWLCLEN 902

Query: 841  GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVIEEWTNEAVENEMDLILSAV 900
            GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSV EEWT+E+VE EMDLI+SAV
Sbjct: 903  GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWTSESVETEMDLIVSAV 962

Query: 901  KSLRSLAKESRERRPGYVLPRNDAVAEYINKRKLEIVTLANLSSLTVINDNDAAPIGCAV 960
            KSLRSLAKESRERRPGY+LPRND VAE INKRKLEIVTLANLSSLTVINDNDAAP+GCAV
Sbjct: 963  KSLRSLAKESRERRPGYMLPRNDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAV 1022

Query: 961  SVVNENLSVYLQFQGAVSVEAELEKIKKKVEEIKKQQEKLKKMMDASGYKEKVRPHIHEE 1020
            SVVNENLSVYLQFQGA+S EAELEKIKKK+EE++KQQEKLKKMMDASGYKEKVRPHIHEE
Sbjct: 1023 SVVNENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEE 1082

Query: 1021 NVNKLASLMQELLSLEEAGQHIQAHR 1047
            NVNKLASLMQELLSLEEAGQHIQAHR
Sbjct: 1083 NVNKLASLMQELLSLEEAGQHIQAHR 1107

BLAST of HG10009470 vs. ExPASy TrEMBL
Match: A0A5D3BM83 (Valyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1728G00290 PE=3 SV=1)

HSP 1 Score: 2020.4 bits (5233), Expect = 0.0e+00
Identity = 987/1026 (96.20%), Postives = 1015/1026 (98.93%), Query Frame = 0

Query: 21   KAREKELKKKKALEKALKLQAQQTSNAAPKKSEKKNARRGGDEENAEDFVDPDTPFGKKK 80
            +AREKELKKKKALEKALKLQAQQTSN APKKSEKKNARRGGDEENAEDFVDPDTPFGKKK
Sbjct: 6    RAREKELKKKKALEKALKLQAQQTSN-APKKSEKKNARRGGDEENAEDFVDPDTPFGKKK 65

Query: 81   LLSQQMAKQYNPSAVEKSWYEWWEKSEFFVADAKSSKPPFVIVLPPPNVTGALHIGHALT 140
            LLSQQMAKQYNPSAVEKSWYEWWEKS +FVADAKSSKPPFVIVLPPPNVTGALHIGHALT
Sbjct: 66   LLSQQMAKQYNPSAVEKSWYEWWEKSGYFVADAKSSKPPFVIVLPPPNVTGALHIGHALT 125

Query: 141  AAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVW 200
            AAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVW
Sbjct: 126  AAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVW 185

Query: 201  DWKTKYGGTILRQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGVIYRDLRLVNW 260
            +WKTKYGGTIL+QLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNG+IYRDLRLVNW
Sbjct: 186  EWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGLIYRDLRLVNW 245

Query: 261  DCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVE 320
            DCVLRTAISDIEVDYIDIKEKTLL+VPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVE
Sbjct: 246  DCVLRTAISDIEVDYIDIKEKTLLRVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVE 305

Query: 321  TMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDAILVDPNFGTGAVKITPAHDP 380
            TMLGDTAIA+HP+DTRYKHLHGK AIHPFNGRKLPIICDAILVDP FGTGAVKITPAHDP
Sbjct: 306  TMLGDTAIAVHPEDTRYKHLHGKSAIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDP 365

Query: 381  NDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAREMVVDALQKKGLYRGAKDNE 440
            NDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAREMVVDALQKKGLYRGAKDNE
Sbjct: 366  NDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAREMVVDALQKKGLYRGAKDNE 425

Query: 441  MRLGLCSRSNDVVEPMIKPQWYVNCKDPAKQSLDAAMDNECKKLDIIPKQYSADWKRWLE 500
            MRLGLCSR+NDVVEPMIKPQWYVNCK+PAKQSLDAAMDNECKK+DIIPKQYSADWKRWL+
Sbjct: 426  MRLGLCSRTNDVVEPMIKPQWYVNCKEPAKQSLDAAMDNECKKIDIIPKQYSADWKRWLD 485

Query: 501  NIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARNEEEAQEEARQIYAGKK 560
            NIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARNEEEA+EEA++IYAGKK
Sbjct: 486  NIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARNEEEAEEEAKRIYAGKK 545

Query: 561  FHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTSALETGHDILFFWVARMVMLG 620
            FHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTSALETGHDILFFWVARMVMLG
Sbjct: 546  FHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTSALETGHDILFFWVARMVMLG 605

Query: 621  ITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPK 680
            ITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPK
Sbjct: 606  ITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPK 665

Query: 681  ELAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAI 740
            ELAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAI
Sbjct: 666  ELAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAI 725

Query: 741  RFAMSKLGNDYVPNKNVTSDVLPFSCQWILSVLNKAISRTVSSLESYEFSDATTAVYSWW 800
            RFAMSKLGNDYVPNKNVT DVLPFSCQWILSVLNKAISRT+SSLESYEFSDATTAVYSWW
Sbjct: 726  RFAMSKLGNDYVPNKNVTPDVLPFSCQWILSVLNKAISRTISSLESYEFSDATTAVYSWW 785

Query: 801  QYQLCDVFIEAIKPYFSSNDTEFASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRL 860
            QYQLCDVFIEAIKPYFSSN+T++ASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRL
Sbjct: 786  QYQLCDVFIEAIKPYFSSNETDYASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRL 845

Query: 861  PYPKSSTRPESIMICDYPSVIEEWTNEAVENEMDLILSAVKSLRSLAKESRERRPGYVLP 920
            PYPK+STRPESIMICDYPSV EEWTNEAVENEMDLI+SAV+SLRSLAKESRER+PGYVLP
Sbjct: 846  PYPKNSTRPESIMICDYPSVTEEWTNEAVENEMDLIVSAVRSLRSLAKESRERKPGYVLP 905

Query: 921  RNDAVAEYINKRKLEIVTLANLSSLTVINDNDAAPIGCAVSVVNENLSVYLQFQGAVSVE 980
            RN AVAE INKRKLEIVTLANLSSLTVINDNDAAP+GCAVSVVNENLSVYLQFQGA+S E
Sbjct: 906  RNLAVAEIINKRKLEIVTLANLSSLTVINDNDAAPVGCAVSVVNENLSVYLQFQGAISAE 965

Query: 981  AELEKIKKKVEEIKKQQEKLKKMMDASGYKEKVRPHIHEENVNKLASLMQELLSLEEAGQ 1040
            AELEKI KK++EIKKQQE+LKKMMDASGYKEKVRP IHEENVNKLASLMQELLSLEEAGQ
Sbjct: 966  AELEKINKKIDEIKKQQERLKKMMDASGYKEKVRPQIHEENVNKLASLMQELLSLEEAGQ 1025

Query: 1041 HIQAHR 1047
            HIQAHR
Sbjct: 1026 HIQAHR 1030

BLAST of HG10009470 vs. ExPASy TrEMBL
Match: A0A0A0KPW4 (Valyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_5G174580 PE=3 SV=1)

HSP 1 Score: 2010.0 bits (5206), Expect = 0.0e+00
Identity = 982/1026 (95.71%), Postives = 1011/1026 (98.54%), Query Frame = 0

Query: 21   KAREKELKKKKALEKALKLQAQQTSNAAPKKSEKKNARRGGDEENAEDFVDPDTPFGKKK 80
            +AREKELKKKKALEKALKLQAQQTSN APKKSEKKNARRGGDEENAEDFVDPDTPFGKKK
Sbjct: 2    QAREKELKKKKALEKALKLQAQQTSN-APKKSEKKNARRGGDEENAEDFVDPDTPFGKKK 61

Query: 81   LLSQQMAKQYNPSAVEKSWYEWWEKSEFFVADAKSSKPPFVIVLPPPNVTGALHIGHALT 140
            LL+QQMAKQYNPSAVEKSWYEWWEKS +FVADAKSSKPPFVIVLPPPNVTGALHIGHALT
Sbjct: 62   LLAQQMAKQYNPSAVEKSWYEWWEKSGYFVADAKSSKPPFVIVLPPPNVTGALHIGHALT 121

Query: 141  AAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVW 200
            AAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHD+GREKFISEVW
Sbjct: 122  AAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDLGREKFISEVW 181

Query: 201  DWKTKYGGTILRQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGVIYRDLRLVNW 260
            +WKTKYGGTIL+QLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNG+IYRDLRLVNW
Sbjct: 182  EWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGLIYRDLRLVNW 241

Query: 261  DCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVE 320
            DCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVE
Sbjct: 242  DCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVE 301

Query: 321  TMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDAILVDPNFGTGAVKITPAHDP 380
            TMLGDTAIAIHP+DTRYKHLHGK AIHPFNGRKLPI+CDAILVDP FGTGAVKITPAHDP
Sbjct: 302  TMLGDTAIAIHPEDTRYKHLHGKSAIHPFNGRKLPIVCDAILVDPKFGTGAVKITPAHDP 361

Query: 381  NDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAREMVVDALQKKGLYRGAKDNE 440
            NDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAREMVVDALQKKGLYRGAKDNE
Sbjct: 362  NDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAREMVVDALQKKGLYRGAKDNE 421

Query: 441  MRLGLCSRSNDVVEPMIKPQWYVNCKDPAKQSLDAAMDNECKKLDIIPKQYSADWKRWLE 500
            MRLGLCSR+NDVVEPMIKPQWYVNCK+PAKQSLDAAMDNECKK+DIIPKQYSADWKRWL+
Sbjct: 422  MRLGLCSRTNDVVEPMIKPQWYVNCKEPAKQSLDAAMDNECKKIDIIPKQYSADWKRWLD 481

Query: 501  NIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARNEEEAQEEARQIYAGKK 560
            NIRDWCISRQLWWGHRIPAWYA LEDDQLKEFGAYNDHWVVARNEEEA+EEA++IYAGKK
Sbjct: 482  NIRDWCISRQLWWGHRIPAWYAALEDDQLKEFGAYNDHWVVARNEEEAEEEAKRIYAGKK 541

Query: 561  FHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTSALETGHDILFFWVARMVMLG 620
            FHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTSALETGHDI+FFWVARMVMLG
Sbjct: 542  FHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTSALETGHDIIFFWVARMVMLG 601

Query: 621  ITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPK 680
            ITLGGDVPF+TIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPK
Sbjct: 602  ITLGGDVPFQTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPK 661

Query: 681  ELAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAI 740
            ELAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAI
Sbjct: 662  ELAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAI 721

Query: 741  RFAMSKLGNDYVPNKNVTSDVLPFSCQWILSVLNKAISRTVSSLESYEFSDATTAVYSWW 800
            RFAMSKLGNDYVPNKNVT D LPFSCQWILSVLNKAISRT+SSLESYEFSDATTAVYSWW
Sbjct: 722  RFAMSKLGNDYVPNKNVTPDALPFSCQWILSVLNKAISRTISSLESYEFSDATTAVYSWW 781

Query: 801  QYQLCDVFIEAIKPYFSSNDTEFASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRL 860
            QYQLCDVFIEAIKPYFSSN+T+FASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRL
Sbjct: 782  QYQLCDVFIEAIKPYFSSNETDFASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRL 841

Query: 861  PYPKSSTRPESIMICDYPSVIEEWTNEAVENEMDLILSAVKSLRSLAKESRERRPGYVLP 920
            PYPK+STRPESIMICDYPSV EEWTNE VENEMDLI+SAV+SLRSLAKESRERRPGYVLP
Sbjct: 842  PYPKNSTRPESIMICDYPSVTEEWTNEDVENEMDLIVSAVRSLRSLAKESRERRPGYVLP 901

Query: 921  RNDAVAEYINKRKLEIVTLANLSSLTVINDNDAAPIGCAVSVVNENLSVYLQFQGAVSVE 980
            RN AVAE INKRKLEIVTLANLSSLTVINDNDAAP+GCAVSVVNENLSVYLQFQGA+S E
Sbjct: 902  RNAAVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAVSVVNENLSVYLQFQGAISAE 961

Query: 981  AELEKIKKKVEEIKKQQEKLKKMMDASGYKEKVRPHIHEENVNKLASLMQELLSLEEAGQ 1040
            AELEKI KK++EIKKQQEKLKKMMDASGYKEKVRP IHEENVNKLASLMQELLSLEEAG 
Sbjct: 962  AELEKINKKMDEIKKQQEKLKKMMDASGYKEKVRPQIHEENVNKLASLMQELLSLEEAGL 1021

Query: 1041 HIQAHR 1047
            HI+AHR
Sbjct: 1022 HIKAHR 1026

BLAST of HG10009470 vs. TAIR 10
Match: AT1G14610.1 (valyl-tRNA synthetase / valine--tRNA ligase (VALRS) )

HSP 1 Score: 1608.6 bits (4164), Expect = 0.0e+00
Identity = 774/1054 (73.43%), Postives = 897/1054 (85.10%), Query Frame = 0

Query: 2    ESDKKPEVEDP-EKKKKKEEKAREKELKKKKALEK----ALKLQAQQTSNAAPKKSEKKN 61
            ES+KK   E+  E+KKKKEEKA+EKELKK+KALEK     LK +  +     PKKS KK+
Sbjct: 47   ESEKKILTEEELERKKKKEEKAKEKELKKQKALEKERLAELKAKQAKDGTNVPKKSAKKS 106

Query: 62   ARRGGDEENAEDFVDPDTPFGKKKLLSQQMAKQYNPSAVEKSWYEWWEKSEFFVADAKSS 121
            ++R   EEN EDFVDP+TP G++K LS QMAKQY+P+ VEKSWY WWEKS+ F ADAKSS
Sbjct: 107  SKRDASEENPEDFVDPETPLGERKRLSSQMAKQYSPATVEKSWYAWWEKSDLFKADAKSS 166

Query: 122  KPPFVIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVE 181
            KPPFVIVLPPPNVTGALHIGHALT+AIED IIRW+RMSGYN LWVPG DHAGIATQVVVE
Sbjct: 167  KPPFVIVLPPPNVTGALHIGHALTSAIEDTIIRWKRMSGYNALWVPGVDHAGIATQVVVE 226

Query: 182  KKIMRERNLTRHDIGREKFISEVWDWKTKYGGTILRQLRRLGASLDWTRECFTMDEKRSR 241
            KKIMR+R +TRHD+GRE+F+ EVW WK +YGGTIL QLRRLGASLDW+RECFTMDE+RS+
Sbjct: 227  KKIMRDRGMTRHDVGREEFVKEVWKWKNQYGGTILTQLRRLGASLDWSRECFTMDEQRSK 286

Query: 242  AVTEAFVRLFKNGVIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFG 301
            AVTEAFVRL+K G+IYRD+RLVNWDC+LRTAISD+EV+YIDIKEKTLLKVPGYE PVEFG
Sbjct: 287  AVTEAFVRLYKEGLIYRDIRLVNWDCILRTAISDVEVEYIDIKEKTLLKVPGYEKPVEFG 346

Query: 302  VLTSFAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPI 361
            +LTSFAYPLEG LGE++VATTRVETMLGDTAIAIHPDD RYKHLHGKFA+HPFNGRKLPI
Sbjct: 347  LLTSFAYPLEGGLGEVIVATTRVETMLGDTAIAIHPDDARYKHLHGKFAVHPFNGRKLPI 406

Query: 362  ICDAILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPR 421
            ICD ILVDPNFGTG VKITPAHDPND EVGKRH LEFINIFTDDGKIN+NGGS+FAGMPR
Sbjct: 407  ICDGILVDPNFGTGCVKITPAHDPNDCEVGKRHKLEFINIFTDDGKINTNGGSDFAGMPR 466

Query: 422  FKAREMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDPAKQSLDAA 481
            F ARE VV+ALQK+GLYRGAK+NEMRLGLCSR+NDV+EPMIKPQWYVNC    K++LD A
Sbjct: 467  FAAREAVVEALQKQGLYRGAKNNEMRLGLCSRTNDVIEPMIKPQWYVNCSMIGKEALDVA 526

Query: 482  MDNECKKLDIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYN 541
            + +E KKL+ +PKQY+A+W+RWLENIRDWCISRQLWWGHRIPAWYATLE+DQLKE GAY+
Sbjct: 527  ITDENKKLEFVPKQYTAEWRRWLENIRDWCISRQLWWGHRIPAWYATLEEDQLKEVGAYS 586

Query: 542  DHWVVARNEEEAQEEARQIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAF 601
            DHWVVAR E++A+EEA Q + GKKF LT+DPDVLDTWFSSGLFPLSVLGWPD T+D KAF
Sbjct: 587  DHWVVARTEDDAREEAAQKFLGKKFELTRDPDVLDTWFSSGLFPLSVLGWPDVTDDFKAF 646

Query: 602  YPTSALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDP 661
            YPTS LETGHDILFFWVARMVM+G+ LGG+VPF  +Y HPMIRDAHGRKMSKSLGNVIDP
Sbjct: 647  YPTSVLETGHDILFFWVARMVMMGMKLGGEVPFSKVYFHPMIRDAHGRKMSKSLGNVIDP 706

Query: 662  VEVINGISLEGLHKRLEEGNLDPKELAIAKEGQVKDFPNGISECGADALRFALISYTAQS 721
            +EVING++LEGLHKRLEEGNLDPKE+ +AKEGQVKDFPNGI ECG DALRFAL+SYTAQS
Sbjct: 707  LEVINGVTLEGLHKRLEEGNLDPKEVIVAKEGQVKDFPNGIPECGTDALRFALVSYTAQS 766

Query: 722  DKINLDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPNKNVTSDVLPFSCQWILSVLNKA 781
            DKINLDI RVVGYRQWCNKLWNA+RFAM KLG+ Y P + ++ + +PFSCQWILSVLNKA
Sbjct: 767  DKINLDILRVVGYRQWCNKLWNAVRFAMMKLGDGYTPPQTLSPETMPFSCQWILSVLNKA 826

Query: 782  ISRTVSSLESYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEFASARSHAQDTLWL 841
            IS+TV SL+++EFSDA   +Y+WWQYQ CDV+IEAIKPYF+ ++  FAS R+HAQ  LW+
Sbjct: 827  ISKTVVSLDAFEFSDAANTIYAWWQYQFCDVYIEAIKPYFAGDNPTFASERAHAQHALWI 886

Query: 842  CLENGLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVIEEWTNEAVENEMDLI 901
             LE GLRLLHPFMP+VTEELWQRLP PK + R  SIMICDYPS IE W+NE VE+EMD +
Sbjct: 887  SLETGLRLLHPFMPFVTEELWQRLPAPKDTERKASIMICDYPSAIENWSNEKVESEMDTV 946

Query: 902  LSAVKSLRS-----LAKESRERRPGYVLPRNDAVAEYINKRKLEIVTLANLSSLTVINDN 961
            L+ VK +R+     L K+  ER P + L  N+  +E +   +LEI TLANLSSL V++  
Sbjct: 947  LATVKCMRALRAGLLEKQKNERLPAFALCENNVTSEIVKSHELEIRTLANLSSLEVVSKG 1006

Query: 962  D-AAPIGCAVSVVNENLSVYLQFQGAVSVEAELEKIKKKVEEIKKQQEKLKKMMDASGYK 1021
              AAP G +V  VNENL VYL+  GA++ EAE EKI+ K+ E++KQ+EKL+KMM  S Y+
Sbjct: 1007 QHAAPPGSSVETVNENLKVYLEVDGAINTEAEQEKIRNKIGELQKQKEKLQKMMSVSTYE 1066

Query: 1022 EKVRPHIHEENVNKLASLMQELLSLEEAGQHIQA 1045
            EKV  +I E+N NKLA ++QE    E+    + A
Sbjct: 1067 EKVPANIKEDNANKLAKILQEFDFFEKESARLAA 1100

BLAST of HG10009470 vs. TAIR 10
Match: AT5G16715.1 (ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases )

HSP 1 Score: 647.1 bits (1668), Expect = 2.4e-185
Identity = 380/980 (38.78%), Postives = 548/980 (55.92%), Query Frame = 0

Query: 77   GKKKLLSQQMAKQYNPSAVEKSWYEWWEKSEFFVADAKSSKPPFVIVLPPPNVTGALHIG 136
            G     S + +K ++ S+ EK  Y+WWE   +F  +      PFVI +PPPNVTG+LH+G
Sbjct: 60   GNNVFTSPETSKTFDFSSEEKI-YKWWESQGYFKPNFDQGGSPFVIPMPPPNVTGSLHMG 119

Query: 137  HALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFI 196
            HA+   +ED ++R+ RM+G  TLW+PGTDHAGIATQ+VVEK +  E  + R D+GR++F 
Sbjct: 120  HAMFVTLEDIMVRYNRMNGRPTLWLPGTDHAGIATQLVVEKMLASE-GIKRVDLGRDEFT 179

Query: 197  SEVWDWKTKYGGTILRQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGVIYRDLR 256
              VW+WK KYGGTI  Q++RLGAS DW+RE FT+DE+ SRAV EAFV+L   G+IY+   
Sbjct: 180  KRVWEWKEKYGGTITNQIKRLGASCDWSRERFTLDEQLSRAVVEAFVKLHDKGLIYQGSY 239

Query: 257  LVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELGEIVVAT 316
            +VNW   L+TA+SD+EV+Y +                E G L    Y + G    + +AT
Sbjct: 240  MVNWSPNLQTAVSDLEVEYSE----------------EPGFLYHIKYRVAGSPDFLTIAT 299

Query: 317  TRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFN-GRKLPIICDAILVDPNFGTGAVKIT 376
            TR ET+ GD A+A+HP+D RY    G+ AI P   GR +PII D   VD +FGTG +KI+
Sbjct: 300  TRPETLFGDVALAVHPEDDRYSKYVGQTAIVPMTYGRHVPIIADK-YVDKDFGTGVLKIS 359

Query: 377  PAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAREMVVDALQKKGLYRG 436
            P HD ND+ + ++  L  +N+   D  +N   G  F G+ RF+ RE +   L++ GL   
Sbjct: 360  PGHDHNDYLLARKLGLPILNVMNKDATLNDVAGL-FCGLDRFEVREKLWADLEEIGLAVK 419

Query: 437  AKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDPAKQSLDAAMDNECKKLDIIPKQYSADW 496
             + + +R+    R  +V+EP++  QW+V+  DP  +    A++N  K+L IIP+++   +
Sbjct: 420  KEPHTLRVPRSQRGGEVIEPLVSKQWFVH-MDPLAEKALLAVEN--KELTIIPERFEKIY 479

Query: 497  KRWLENIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARNEEEAQEEARQI 556
              WL NI+DWCISRQLWWGHRIP WY   +D +        + ++VA++ EEA E+A + 
Sbjct: 480  NHWLTNIKDWCISRQLWWGHRIPVWYVVGKDCE--------EDYIVAKSAEEALEKALEK 539

Query: 557  YAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPD-DTEDLKAFYPTSALETGHDILFFWVA 616
            Y GK   + QDPDVLDTWFSS L+P S LGWPD   +D   FYPT+ LETGHDILFFWVA
Sbjct: 540  Y-GKDVEIYQDPDVLDTWFSSSLWPFSTLGWPDVAAKDFNNFYPTNMLETGHDILFFWVA 599

Query: 617  RMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEE 676
            RMVM+GI   G VPF  +YLH +IRD+ GRKMSKSLGNVIDP++ I              
Sbjct: 600  RMVMMGIEFTGTVPFSHVYLHGLIRDSQGRKMSKSLGNVIDPLDTI-------------- 659

Query: 677  GNLDPKELAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCN 736
                            KDF       G DALRF +   TA  D +NL  +R+   + + N
Sbjct: 660  ----------------KDF-------GTDALRFTIALGTAGQD-LNLSTERLTANKAFTN 719

Query: 737  KLWNAIRFAMSKLGN-----------DYVPNKNVTSDVLPFSCQWILSVLNKAISRTVSS 796
            KLWNA +F +  L +           D   +K  T   LP    W +S L+  I    +S
Sbjct: 720  KLWNAGKFVLHSLPSLSDTSAWENLLDLKLDKEETLLSLPLPECWAVSKLHILIDSVTAS 779

Query: 797  LESYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEFASARSHAQDTLWLCLENGLR 856
             E   F D     Y ++     D +IEA K     +     S  S A   L    EN L+
Sbjct: 780  YEKLFFGDVGRETYDFFWSDFADWYIEASKSRLYGSGGNSVSLASQA--VLLYVFENILK 839

Query: 857  LLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVIEEWTNEAVE--NEMDLILSAVK 916
            LLHPFMP+VTE+LWQ LPY     R E++++  +P        E+++    +  +  A++
Sbjct: 840  LLHPFMPFVTEDLWQALPY-----RKEALIVSPWPQNSLPRNVESIKRFENLQALTRAIR 899

Query: 917  SLRSLAKESRERRPGYVLPRNDAVAEYINKRKLEIVTLANLSSLTVINDNDAAPIGCAVS 976
            + R+       +R    +  +  V EYI+K K E++ L +   L  ++ ++A P    +S
Sbjct: 900  NARAEYSVEPVKRISASVVGSAEVIEYISKEK-EVLALLSRLDLNNVHFSNAPPGDANLS 959

Query: 977  ---VVNENLSVYLQFQGAVSVEAELEKIKKKVEEIKKQQEKLKKMMDASGYKEKVRPHIH 1036
               V +E L  YL     V + +E+++I K++ +++ + + L   + +  + EK  P   
Sbjct: 960  VHLVASEGLEAYLPLAAMVDISSEVQRISKRLSKMQTEYDALITRLSSPKFVEKA-PEEV 960

Query: 1037 EENVNKLASLMQELLSLEEA 1039
               V +    ++E + L +A
Sbjct: 1020 VRGVKEQVEELEEKIKLTKA 960

BLAST of HG10009470 vs. TAIR 10
Match: AT5G49030.1 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 187.6 bits (475), Expect = 5.2e-47
Identity = 206/922 (22.34%), Postives = 342/922 (37.09%), Query Frame = 0

Query: 48  APKKSEKKNARRGGDEENAEDFVDPDTPFGKKKLLSQQMAKQYNPSAVEKSWYEWWEKSE 107
           A KK+ +   +  G  +   D   P T FG +           N    E    + WE+++
Sbjct: 87  AAKKASEGEKQEDGKYKQTVDL--PKTGFGMRA----------NSLTREPELQKLWEENQ 146

Query: 108 FF--VADAKSSKPPFVIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTD 167
            F  V+D  ++   F++   PP   G LH+GHAL   ++D I R++ +  Y   +VPG D
Sbjct: 147 VFKRVSD-NNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKVQYVPGWD 206

Query: 168 HAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWDWKTKYGGTILRQLRRLGASLDWTR 227
             G+  ++ V + + +E    R ++   K  ++   +      T +   +R G   DW  
Sbjct: 207 CHGLPIELKVLQSLDQE---VRKELTPLKLRAKAAKFAKATVKTQMESFKRFGVWADWNN 266

Query: 228 ECFTMDEKRSRAVTEAFVRLFKNGVIYRDLRLVNWDCVLRTAISDIEVDYIDIK-EKTLL 287
              T+D +   A  E F ++   G IYR  + V+W    RTA+++ E++Y +    K++ 
Sbjct: 267 PYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAELEYPEGHISKSIY 326

Query: 288 KVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVETMLGDTAIAIHPD----------- 347
            +       +  +L  F   +      + V TT   TM  + A+A++             
Sbjct: 327 AIFKLVGGAKTSLLDEFIPNI-----YLAVWTTTPWTMPANAAVAVNAKLQYSVVEVQSF 386

Query: 348 -------DTRYKHLHGK--------FAI-------------------------------- 407
                   +  K + GK        F I                                
Sbjct: 387 SEDESTVTSNKKKIPGKVLKNQQKLFVIVATDLVPALEAKWGVKLSISKTFLGSDLENCR 446

Query: 408 --HPFNGRKLPIICDAILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKIN 467
             HP + R  P++     +    GTG V   P H   D+  G ++ L  ++   D+GK  
Sbjct: 447 YTHPIDNRDCPVVIGGDYITTESGTGLVHTAPGHGQEDYATGLKYGLPLVSPVDDEGKFT 506

Query: 468 SNGGSEFAGMPRF-KAREMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYV 527
              G +F G+    +    VV  L +       +    +     R+          QW+ 
Sbjct: 507 EEAG-QFRGLSVLGEGNTAVVSYLDENMSLVMEESYAHKYPYDWRTKKPTIFRATEQWFA 566

Query: 528 NCKDPAKQSLDAAMDNECKKLDIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYAT 587
           + +     ++DA        +  +P Q          +  DWCISRQ  WG  IPA+Y  
Sbjct: 567 SVEGFRTATMDA-----INNVKWVPHQAVNRISAMTSSRSDWCISRQRTWGVPIPAFYHV 626

Query: 588 LEDDQLKEFGAYN-----------DHWVVARNEEEAQEEARQIYAGKKFHLTQDPDVLDT 647
              + L      N           D W     E+   E+    Y  K     +  D +D 
Sbjct: 627 KTKEPLMNEETINHVKSIISQKGSDAWWYMSVEDLLPEK----YRDKAADYEKGTDTMDV 686

Query: 648 WFSSGLFPLSVLGWPDDTEDLKAFYPTSALETGHDILFFWVARMVMLGITLGGDVPFRTI 707
           WF SG     VLG     E L   +P      G D    W    ++  I   G  P+  +
Sbjct: 687 WFDSGSSWAGVLG---KREGLS--FPADVYLEGTDQHRGWFQSSLLTSIATQGKAPYSAV 746

Query: 708 YLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKELAIAKEGQVKD 767
             H  + D  G KMSKSLGNV+DP  VI G                            KD
Sbjct: 747 ITHGFVLDEKGMKMSKSLGNVVDPRLVIEG------------------------GKNSKD 806

Query: 768 FPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYV 827
            P      GAD +R  + S     D + +  Q +        KL   +R+ +  L +D+ 
Sbjct: 807 AP----AYGADVMRLWVSSVDYTGD-VLIGPQILRQMSDIYRKLRGTLRYLLGNL-HDWR 866

Query: 828 PNKNVTSDVLPFSCQWILSVLNKAISRTVSSLESYEFSDATTAVYSWWQYQLCDVFIEAI 887
            +  V    LP   Q  L  L   +       E+Y+F      +  +    L + + +  
Sbjct: 867 VDNAVPYQDLPIIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFYFDIA 926

Query: 888 KPYFSSNDTEFASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRLPYP---KSSTRP 892
           K    +  T   + RS  Q  L   L + LR++ P +P++ E++WQ LP+    +  +  
Sbjct: 927 KDRLYTGGTSSFTRRS-CQTVLSTHLLSILRVIAPIVPHLAEDVWQNLPFEYRNEDGSAA 941

BLAST of HG10009470 vs. TAIR 10
Match: AT5G49030.2 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 187.6 bits (475), Expect = 5.2e-47
Identity = 206/922 (22.34%), Postives = 342/922 (37.09%), Query Frame = 0

Query: 48  APKKSEKKNARRGGDEENAEDFVDPDTPFGKKKLLSQQMAKQYNPSAVEKSWYEWWEKSE 107
           A KK+ +   +  G  +   D   P T FG +           N    E    + WE+++
Sbjct: 87  AAKKASEGEKQEDGKYKQTVDL--PKTGFGMRA----------NSLTREPELQKLWEENQ 146

Query: 108 FF--VADAKSSKPPFVIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTD 167
            F  V+D  ++   F++   PP   G LH+GHAL   ++D I R++ +  Y   +VPG D
Sbjct: 147 VFKRVSD-NNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKVQYVPGWD 206

Query: 168 HAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWDWKTKYGGTILRQLRRLGASLDWTR 227
             G+  ++ V + + +E    R ++   K  ++   +      T +   +R G   DW  
Sbjct: 207 CHGLPIELKVLQSLDQE---VRKELTPLKLRAKAAKFAKATVKTQMESFKRFGVWADWNN 266

Query: 228 ECFTMDEKRSRAVTEAFVRLFKNGVIYRDLRLVNWDCVLRTAISDIEVDYIDIK-EKTLL 287
              T+D +   A  E F ++   G IYR  + V+W    RTA+++ E++Y +    K++ 
Sbjct: 267 PYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAELEYPEGHISKSIY 326

Query: 288 KVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVETMLGDTAIAIHPD----------- 347
            +       +  +L  F   +      + V TT   TM  + A+A++             
Sbjct: 327 AIFKLVGGAKTSLLDEFIPNI-----YLAVWTTTPWTMPANAAVAVNAKLQYSVVEVQSF 386

Query: 348 -------DTRYKHLHGK--------FAI-------------------------------- 407
                   +  K + GK        F I                                
Sbjct: 387 SEDESTVTSNKKKIPGKVLKNQQKLFVIVATDLVPALEAKWGVKLSISKTFLGSDLENCR 446

Query: 408 --HPFNGRKLPIICDAILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKIN 467
             HP + R  P++     +    GTG V   P H   D+  G ++ L  ++   D+GK  
Sbjct: 447 YTHPIDNRDCPVVIGGDYITTESGTGLVHTAPGHGQEDYATGLKYGLPLVSPVDDEGKFT 506

Query: 468 SNGGSEFAGMPRF-KAREMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYV 527
              G +F G+    +    VV  L +       +    +     R+          QW+ 
Sbjct: 507 EEAG-QFRGLSVLGEGNTAVVSYLDENMSLVMEESYAHKYPYDWRTKKPTIFRATEQWFA 566

Query: 528 NCKDPAKQSLDAAMDNECKKLDIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYAT 587
           + +     ++DA        +  +P Q          +  DWCISRQ  WG  IPA+Y  
Sbjct: 567 SVEGFRTATMDA-----INNVKWVPHQAVNRISAMTSSRSDWCISRQRTWGVPIPAFYHV 626

Query: 588 LEDDQLKEFGAYN-----------DHWVVARNEEEAQEEARQIYAGKKFHLTQDPDVLDT 647
              + L      N           D W     E+   E+    Y  K     +  D +D 
Sbjct: 627 KTKEPLMNEETINHVKSIISQKGSDAWWYMSVEDLLPEK----YRDKAADYEKGTDTMDV 686

Query: 648 WFSSGLFPLSVLGWPDDTEDLKAFYPTSALETGHDILFFWVARMVMLGITLGGDVPFRTI 707
           WF SG     VLG     E L   +P      G D    W    ++  I   G  P+  +
Sbjct: 687 WFDSGSSWAGVLG---KREGLS--FPADVYLEGTDQHRGWFQSSLLTSIATQGKAPYSAV 746

Query: 708 YLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKELAIAKEGQVKD 767
             H  + D  G KMSKSLGNV+DP  VI G                            KD
Sbjct: 747 ITHGFVLDEKGMKMSKSLGNVVDPRLVIEG------------------------GKNSKD 806

Query: 768 FPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYV 827
            P      GAD +R  + S     D + +  Q +        KL   +R+ +  L +D+ 
Sbjct: 807 AP----AYGADVMRLWVSSVDYTGD-VLIGPQILRQMSDIYRKLRGTLRYLLGNL-HDWR 866

Query: 828 PNKNVTSDVLPFSCQWILSVLNKAISRTVSSLESYEFSDATTAVYSWWQYQLCDVFIEAI 887
            +  V    LP   Q  L  L   +       E+Y+F      +  +    L + + +  
Sbjct: 867 VDNAVPYQDLPIIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFYFDIA 926

Query: 888 KPYFSSNDTEFASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRLPYP---KSSTRP 892
           K    +  T   + RS  Q  L   L + LR++ P +P++ E++WQ LP+    +  +  
Sbjct: 927 KDRLYTGGTSSFTRRS-CQTVLSTHLLSILRVIAPIVPHLAEDVWQNLPFEYRNEDGSAA 941

BLAST of HG10009470 vs. TAIR 10
Match: AT5G49030.3 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 187.6 bits (475), Expect = 5.2e-47
Identity = 206/922 (22.34%), Postives = 342/922 (37.09%), Query Frame = 0

Query: 48   APKKSEKKNARRGGDEENAEDFVDPDTPFGKKKLLSQQMAKQYNPSAVEKSWYEWWEKSE 107
            A KK+ +   +  G  +   D   P T FG +           N    E    + WE+++
Sbjct: 273  AAKKASEGEKQEDGKYKQTVDL--PKTGFGMRA----------NSLTREPELQKLWEENQ 332

Query: 108  FF--VADAKSSKPPFVIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTD 167
             F  V+D  ++   F++   PP   G LH+GHAL   ++D I R++ +  Y   +VPG D
Sbjct: 333  VFKRVSD-NNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKVQYVPGWD 392

Query: 168  HAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWDWKTKYGGTILRQLRRLGASLDWTR 227
              G+  ++ V + + +E    R ++   K  ++   +      T +   +R G   DW  
Sbjct: 393  CHGLPIELKVLQSLDQE---VRKELTPLKLRAKAAKFAKATVKTQMESFKRFGVWADWNN 452

Query: 228  ECFTMDEKRSRAVTEAFVRLFKNGVIYRDLRLVNWDCVLRTAISDIEVDYIDIK-EKTLL 287
               T+D +   A  E F ++   G IYR  + V+W    RTA+++ E++Y +    K++ 
Sbjct: 453  PYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAELEYPEGHISKSIY 512

Query: 288  KVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVETMLGDTAIAIHPD----------- 347
             +       +  +L  F   +      + V TT   TM  + A+A++             
Sbjct: 513  AIFKLVGGAKTSLLDEFIPNI-----YLAVWTTTPWTMPANAAVAVNAKLQYSVVEVQSF 572

Query: 348  -------DTRYKHLHGK--------FAI-------------------------------- 407
                    +  K + GK        F I                                
Sbjct: 573  SEDESTVTSNKKKIPGKVLKNQQKLFVIVATDLVPALEAKWGVKLSISKTFLGSDLENCR 632

Query: 408  --HPFNGRKLPIICDAILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKIN 467
              HP + R  P++     +    GTG V   P H   D+  G ++ L  ++   D+GK  
Sbjct: 633  YTHPIDNRDCPVVIGGDYITTESGTGLVHTAPGHGQEDYATGLKYGLPLVSPVDDEGKFT 692

Query: 468  SNGGSEFAGMPRF-KAREMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYV 527
               G +F G+    +    VV  L +       +    +     R+          QW+ 
Sbjct: 693  EEAG-QFRGLSVLGEGNTAVVSYLDENMSLVMEESYAHKYPYDWRTKKPTIFRATEQWFA 752

Query: 528  NCKDPAKQSLDAAMDNECKKLDIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYAT 587
            + +     ++DA        +  +P Q          +  DWCISRQ  WG  IPA+Y  
Sbjct: 753  SVEGFRTATMDA-----INNVKWVPHQAVNRISAMTSSRSDWCISRQRTWGVPIPAFYHV 812

Query: 588  LEDDQLKEFGAYN-----------DHWVVARNEEEAQEEARQIYAGKKFHLTQDPDVLDT 647
               + L      N           D W     E+   E+    Y  K     +  D +D 
Sbjct: 813  KTKEPLMNEETINHVKSIISQKGSDAWWYMSVEDLLPEK----YRDKAADYEKGTDTMDV 872

Query: 648  WFSSGLFPLSVLGWPDDTEDLKAFYPTSALETGHDILFFWVARMVMLGITLGGDVPFRTI 707
            WF SG     VLG     E L   +P      G D    W    ++  I   G  P+  +
Sbjct: 873  WFDSGSSWAGVLG---KREGLS--FPADVYLEGTDQHRGWFQSSLLTSIATQGKAPYSAV 932

Query: 708  YLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKELAIAKEGQVKD 767
              H  + D  G KMSKSLGNV+DP  VI G                            KD
Sbjct: 933  ITHGFVLDEKGMKMSKSLGNVVDPRLVIEG------------------------GKNSKD 992

Query: 768  FPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYV 827
             P      GAD +R  + S     D + +  Q +        KL   +R+ +  L +D+ 
Sbjct: 993  AP----AYGADVMRLWVSSVDYTGD-VLIGPQILRQMSDIYRKLRGTLRYLLGNL-HDWR 1052

Query: 828  PNKNVTSDVLPFSCQWILSVLNKAISRTVSSLESYEFSDATTAVYSWWQYQLCDVFIEAI 887
             +  V    LP   Q  L  L   +       E+Y+F      +  +    L + + +  
Sbjct: 1053 VDNAVPYQDLPIIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFYFDIA 1112

Query: 888  KPYFSSNDTEFASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRLPYP---KSSTRP 892
            K    +  T   + RS  Q  L   L + LR++ P +P++ E++WQ LP+    +  +  
Sbjct: 1113 KDRLYTGGTSSFTRRS-CQTVLSTHLLSILRVIAPIVPHLAEDVWQNLPFEYRNEDGSAA 1127