HG10009466 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10009466
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	mitogen-activated protein kinase kinase kinase 17-like
Location	Chr06: 6237310 .. 6238078 (+)
RNA-Seq Expression	HG10009466
Synteny	HG10009466

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAGAAGAAACTATTTTGCTCAATTTCAAAGGATGTCCTGAAATTGTCCAATCTCTCGAAAGTGGTATCTGTTTTGAGGGTGGTTCATTGGATTCAGTTTCTTATAATCTTGTGTTGGAGTACGCTGCGAGAGGTAGTTTAACTAAATTGCCAGAGACCGAGGTCAAGGACTATATTAGAATGATCGTGAAAGGCCTTAGTTATATTCATGAACAAGGGTATGTACATGGCGATCTAAAACCAGATAATATTCTTGTGTTCCCCAATGATAATGGCAAGTTGAAGTTAAAGATAGCTGACTTTGGGCTTTCCAAGAGATGTGATGAGTTTGAGGAAGTGGTGATATCCTCTGATGCAAACCAAGATTTAGAGGAACACCAAAATATATGTCACCATAATCTATTCTTTTCGGTAAGATCAACACTTCACTTGATATTTGGTCTTTGGGTTGTATTTTGATTGAAATGATTTCTGGAAAGCGGGCGCGGAGTGATTGTAAAACTGTGGGACAACTTGTAAAAAGGTAGTGAATCAAATGGATCTACCGGACATTCCAGATGAATTATCAAGCGAAGGAAGAGATTTTCTTTCAAAATGTATATATCAAAACTCTGAACAAAGATGGACGGCTAATATGCTACTCAAACATCCCTATCTCAAAGAAGAATGTAAAGTTGACGAATTGCTTTCGACTTGGAATTTGTATGATTTATTAGATATAGAAAGTTTCAAAGAAAAAGATTTGCATGGAGTTATTTTGAGCTAA

mRNA sequence

ATGACAGAAGAAACTATTTTGCTCAATTTCAAAGGATGTCCTGAAATTGTCCAATCTCTCGAAAGTGGTATCTGTTTTGAGGGTGGTTCATTGGATTCAGTTTCTTATAATCTTGTGTTGGAGTACGCTGCGAGAGGTAGTTTAACTAAATTGCCAGAGACCGAGGTCAAGGACTATATTAGAATGATCGTGAAAGGCCTTAGTTATATTCATGAACAAGGGTATGTACATGGCGATCTAAAACCAGATAATATTCTTGTGTTCCCCAATGATAATGGCAAGTTGAAGTTAAAGATAGCTGACTTTGGGCTTTCCAAGAGATGTGATGAGTTTGAGGAAGTGGTGATATCCTCTGATGCAAACCAAGATTTAGAGGAACACCAAAATATATGTCACCATAATCTATTCTTTTCGGTAGTGAATCAAATGGATCTACCGGACATTCCAGATGAATTATCAAGCGAAGGAAGAGATTTTCTTTCAAAATGTATATATCAAAACTCTGAACAAAGATGGACGGCTAATATGCTACTCAAACATCCCTATCTCAAAGAAGAATGTAAAGTTGACGAATTGCTTTCGACTTGGAATTTGTATGATTTATTAGATATAGAAAGTTTCAAAGAAAAAGATTTGCATGGAGTTATTTTGAGCTAA

Coding sequence (CDS)

ATGACAGAAGAAACTATTTTGCTCAATTTCAAAGGATGTCCTGAAATTGTCCAATCTCTCGAAAGTGGTATCTGTTTTGAGGGTGGTTCATTGGATTCAGTTTCTTATAATCTTGTGTTGGAGTACGCTGCGAGAGGTAGTTTAACTAAATTGCCAGAGACCGAGGTCAAGGACTATATTAGAATGATCGTGAAAGGCCTTAGTTATATTCATGAACAAGGGTATGTACATGGCGATCTAAAACCAGATAATATTCTTGTGTTCCCCAATGATAATGGCAAGTTGAAGTTAAAGATAGCTGACTTTGGGCTTTCCAAGAGATGTGATGAGTTTGAGGAAGTGGTGATATCCTCTGATGCAAACCAAGATTTAGAGGAACACCAAAATATATGTCACCATAATCTATTCTTTTCGGTAGTGAATCAAATGGATCTACCGGACATTCCAGATGAATTATCAAGCGAAGGAAGAGATTTTCTTTCAAAATGTATATATCAAAACTCTGAACAAAGATGGACGGCTAATATGCTACTCAAACATCCCTATCTCAAAGAAGAATGTAAAGTTGACGAATTGCTTTCGACTTGGAATTTGTATGATTTATTAGATATAGAAAGTTTCAAAGAAAAAGATTTGCATGGAGTTATTTTGAGCTAA

Protein sequence

MTEETILLNFKGCPEIVQSLESGICFEGGSLDSVSYNLVLEYAARGSLTKLPETEVKDYIRMIVKGLSYIHEQGYVHGDLKPDNILVFPNDNGKLKLKIADFGLSKRCDEFEEVVISSDANQDLEEHQNICHHNLFFSVVNQMDLPDIPDELSSEGRDFLSKCIYQNSEQRWTANMLLKHPYLKEECKVDELLSTWNLYDLLDIESFKEKDLHGVILS

Homology

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BLAST of HG10009466 vs. NCBI nr
Match: XP_038896352.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 280.4 bits (716), Expect = 1.3e-71
Identity = 154/259 (59.46%), Postives = 178/259 (68.73%), Query Frame = 0


Query: 1   MTEETILLNFKGCPEIVQSLESGICFEGGSLDSVSYNLVLEYAARGSLT-------KLPE 60
           M EETILLNF GCPEI+Q + SGICFEG SLD+V+YNL LEYA  GSLT       KLPE
Sbjct: 45  MIEETILLNFNGCPEIIQRVGSGICFEGDSLDTVTYNLALEYATGGSLTDLIRRREKLPE 104

Query: 61  TEVKDYIRMIVKGLSYIHEQGYVHGDLKPDNILVFPNDNGKLKLKIADFGLSKRCDEFEE 120
            EVK+Y+RMI++GL  IHE+GYVHGD+KPDNILVFP+D GK+KLKIADFGLSK+CDEFEE
Sbjct: 105 VEVKEYLRMILRGLCCIHERGYVHGDIKPDNILVFPDDTGKMKLKIADFGLSKKCDEFEE 164

Query: 121 VVISSDA---------------------NQDLEEHQNIC-----------------HHNL 180
           VVIS++A                     N  L+     C                    L
Sbjct: 165 VVISTNAKPRFRGTPKYMSPESILFGMINTSLDIWSLGCILIEMVSGKQVWSDCKTLGQL 224

Query: 181 FFSVVNQMDLP-DIPDELSSEGRDFLSKCIYQNSEQRWTANMLLKHPYLKEECKVDELLS 214
              VVNQMDLP DIPDELS EGRDFLSKCI Q  EQRWTANMLLKHPYLKEE +VDEL S
Sbjct: 225 AKKVVNQMDLPADIPDELSREGRDFLSKCIDQKFEQRWTANMLLKHPYLKEEYEVDELPS 284

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BLAST of HG10009466 vs. NCBI nr
Match: XP_038896342.1 (mitogen-activated protein kinase kinase kinase 3-like [Benincasa hispida])

HSP 1 Score: 268.5 bits (685), Expect = 5.2e-68
Identity = 149/256 (58.20%), Postives = 172/256 (67.19%), Query Frame = 0


Query: 1   MTEETILLNFKGCPEIVQSLESGICFEGGSLDSVSYNLVLEYAARGSL-------TKLPE 60
           M EETILLNF GCPEI+Q + SGICFEG SLD+V+YNL LEY A GSL        K PE
Sbjct: 45  MIEETILLNFNGCPEIIQRVGSGICFEGDSLDTVTYNLTLEYDAGGSLIDLIRRREKFPE 104

Query: 61  TEVKDYIRMIVKGLSYIHEQGYVHGDLKPDNILVFPNDNGKLKLKIADFGLSKRCDEFEE 120
            EVK+Y++MI++GL  IHE+GYVHGD+K DNILVFP+D GK+KLKIA+FGLSK+CDEFEE
Sbjct: 105 VEVKEYLKMILRGLCCIHERGYVHGDIKVDNILVFPDDTGKVKLKIANFGLSKKCDEFEE 164

Query: 121 VVISSDA---------------------NQDLEEHQNIC-----------------HHNL 180
           V IS++A                     N  L+     C                    L
Sbjct: 165 VAISTNAKPRFRGTPKYISPESILFGKINTSLDIWSLGCVLIEMIYGKPEWSDCKTLGQL 224

Query: 181 FFSVVNQMDLP-DIPDELSSEGRDFLSKCIYQNSEQRWTANMLLKHPYLKEECKVDELLS 211
              +VNQMDLP DIP+ELS EGRDFLSKCI Q SEQRWTANMLLKHPYLKEE KVDEL S
Sbjct: 225 VKKIVNQMDLPADIPEELSREGRDFLSKCIDQKSEQRWTANMLLKHPYLKEEYKVDELFS 284

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BLAST of HG10009466 vs. NCBI nr
Match: XP_038896344.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 268.1 bits (684), Expect = 6.7e-68
Identity = 149/251 (59.36%), Postives = 170/251 (67.73%), Query Frame = 0


Query: 1   MTEETILLNFKGCPEIVQSLESGICFEGGSLDSVSYNLVLEYAARGSLT-------KLPE 60
           M EETILLNF GC EI+Q + SGICFEG SLD+V+YNL LEYA  G+LT       KLPE
Sbjct: 45  MIEETILLNFNGCSEIIQRVGSGICFEGDSLDTVTYNLALEYAVGGNLTDLIRRREKLPE 104

Query: 61  TEVKDYIRMIVKGLSYIHEQGYVHGDLKPDNILVFPNDNGKLKLKIADFGLSKRCDEFEE 120
            EVK+Y+RMI++GL  IHE+GYVHGD+KPDNILVFP+D GK+KLKIADFGLSK+CDEFEE
Sbjct: 105 VEVKEYLRMILRGLCCIHERGYVHGDIKPDNILVFPDDTGKVKLKIADFGLSKKCDEFEE 164

Query: 121 VVISSDA---------------------NQDLEEHQNIC-----------------HHNL 180
           VVIS++A                     N  L+     C                    L
Sbjct: 165 VVISTNAKPRFRGTPKYMSPESILFGKINTSLDIWSLGCVLIEMISGKPVWSDCKTLGQL 224

Query: 181 FFSVVNQMDLP-DIPDELSSEGRDFLSKCIYQNSEQRWTANMLLKHPYLKEECKVDELLS 206
              ++NQMDLP DIP+ELS EGRDFLSKCI Q SEQRWTANMLLKHPYLKEE KVDELL 
Sbjct: 225 AKKILNQMDLPADIPEELSREGRDFLSKCIDQKSEQRWTANMLLKHPYLKEEYKVDELLL 284

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BLAST of HG10009466 vs. NCBI nr
Match: XP_038896343.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 224.2 bits (570), Expect = 1.1e-54
Identity = 129/227 (56.83%), Postives = 150/227 (66.08%), Query Frame = 0


Query: 30  SLDSVSYNLVLEYAARGSL-------TKLPETEVKDYIRMIVKGLSYIHEQGYVHGDLKP 89
           SLD+V+YNL LEYAA GSL        KLPE EVK+Y+RMI++GL  IHE+GYVHGD+KP
Sbjct: 36  SLDTVTYNLALEYAAEGSLIDLIRRREKLPEVEVKEYLRMILRGLCCIHERGYVHGDIKP 95

Query: 90  DNILVFPNDNGKLKLKIADFGLSKRCDEFEEVVISSDA---------------------N 149
           DNILVF +D GK+KLKIA+FGLSK+ DEFEEVVIS++A                     N
Sbjct: 96  DNILVFSDDTGKVKLKIANFGLSKKYDEFEEVVISTNAKPRFRGTPKYMSPESILFGKIN 155

Query: 150 QDLEEHQNIC-----------------HHNLFFSVVNQMDLP-DIPDELSSEGRDFLSKC 209
             L+     C                    L   +VNQMDLP DIP+ELS +GRDFLSKC
Sbjct: 156 TSLDIWSLGCVLIEMISGKPVWSDCKTLGQLMKKIVNQMDLPADIPEELSRKGRDFLSKC 215

Query: 210 IYQNSEQRWTANMLLKHPYLKEECKVDELLSTWNLYDLLDIESFKEK 211
           I Q SEQRWTANMLLKHPYLKEE KVDEL ST +L DL DIE F+E+
Sbjct: 216 IDQKSEQRWTANMLLKHPYLKEEYKVDELFSTQSLPDLSDIEWFEER 262

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BLAST of HG10009466 vs. NCBI nr
Match: XP_038896328.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 187.6 bits (475), Expect = 1.2e-43
Identity = 91/127 (71.65%), Postives = 108/127 (85.04%), Query Frame = 0


Query: 1   MTEETILLNFKGCPEIVQSLESGICFEGGSLDSVSYNLVLEYAARGSLT-------KLPE 60
           M EETILLNF GCPEI+Q + SGICFEG SLD+V+YNL LEYAA GSLT       KLPE
Sbjct: 45  MIEETILLNFNGCPEIIQRVGSGICFEGDSLDTVTYNLALEYAAGGSLTDLIRRREKLPE 104

Query: 61  TEVKDYIRMIVKGLSYIHEQGYVHGDLKPDNILVFPNDNGKLKLKIADFGLSKRCDEFEE 120
            EVK+Y++MI++GL  IHE+GYVHGD+KPDNILVF +D GK+KLKIADFGLSK+CDEFEE
Sbjct: 105 VEVKEYLKMILRGLCCIHERGYVHGDIKPDNILVFLDDIGKVKLKIADFGLSKKCDEFEE 164

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BLAST of HG10009466 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 2.4e-20
Identity = 72/234 (30.77%), Postives = 115/234 (49.15%), Query Frame = 0


Query: 3   EETILLNFKGCPEIVQSLESGICFEGGSLDSVSYNLVLEYAARGSLTK---------LPE 62
           E+++L +   CPEI++        E G      +NL+LEYA+RGSL           LPE
Sbjct: 49  EKSVLDSLGDCPEIIRCYGEDSTVENG---EEMHNLLLEYASRGSLASYMKKLGGEGLPE 108

Query: 63  TEVKDYIRMIVKGLSYIHEQGYVHGDLKPDNILVFPNDNGKLKLKIADFGLSKRCD---- 122
           + V+ +   +++GL +IH +G+ H D+K  NIL+F ND     +KIADFGL+ R D    
Sbjct: 109 STVRRHTGSVLRGLRHIHAKGFAHCDIKLANILLF-NDG---SVKIADFGLAMRVDGDLT 168

Query: 123 --------EFEEVVISSDANQDLEEHQ---------------------NICHHNLFFSVV 182
                       + ++ +   D E                        ++   + F S++
Sbjct: 169 ALRKSVEIRGTPLYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLL 228

Query: 183 NQM----DLPDIPDELSSEGRDFLSKCIYQNSEQRWTANMLLKHPYLKEECKVD 191
            ++    +LP IP+ LS EG+DFLSKC  ++  +RWTA MLL H ++  + + D
Sbjct: 229 IRIGVGDELPKIPEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDD 275

[Close]

BLAST of HG10009466 vs. ExPASy Swiss-Prot
Match: F4HRJ4 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK3 PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 6.0e-19
Identity = 68/227 (29.96%), Postives = 108/227 (47.58%), Query Frame = 0


Query: 1   MTEETILLNFKGCPEIVQSLESGICFEGGSLDSVSYNLVLEYAARGSLTKL-------PE 60
           + +E  LLN    P IVQ       + G  L   + ++ LEY + GS+ KL        E
Sbjct: 261 LNQEINLLNQLCHPNIVQ-------YYGSELSEETLSVYLEYVSGGSIHKLLKDYGSFTE 320

Query: 61  TEVKDYIRMIVKGLSYIHEQGYVHGDLKPDNILVFPNDNGKLKLKIADFGLSKRCDEFE- 120
             +++Y R I+ GL+Y+H +  VH D+K  NILV PN     ++K+ADFG++K    F  
Sbjct: 321 PVIQNYTRQILAGLAYLHGRNTVHRDIKGANILVDPNG----EIKLADFGMAKHVTAFST 380

Query: 121 -------------EVVISSDANQDLEEHQNICHHNL-----------------FFSVVNQ 180
                        EVV+S +      +  ++    L                  F + N 
Sbjct: 381 MLSFKGSPYWMAPEVVMSQNGYTHAVDIWSLGCTILEMATSKPPWSQFEGVAAIFKIGNS 440

Query: 181 MDLPDIPDELSSEGRDFLSKCIYQNSEQRWTANMLLKHPYLKEECKV 190
            D P+IPD LS++ ++F+  C+ +N   R TA+ LL+HP+L+   +V
Sbjct: 441 KDTPEIPDHLSNDAKNFIRLCLQRNPTVRPTASQLLEHPFLRNTTRV 476

[Close]

BLAST of HG10009466 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.0e-18
Identity = 64/201 (31.84%), Postives = 100/201 (49.75%), Query Frame = 0


Query: 34  VSYNLVLEYAARGSLT--------KLPETEVKDYIRMIVKGLSYIHEQGYVHGDLKPDNI 93
           V YNL++EYA  G+LT        ++ ET V  Y R I+KGL YIH +G VH D+K  N+
Sbjct: 73  VMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLEYIHSKGIVHCDVKGSNV 132

Query: 94  LVFPNDNGKLKLKIADFGLSKRCDE-FEEVVISSDA--------NQDLEEHQNI----CH 153
           ++    + K + KIADFG +KR D  FE  V+ + A         +   +  +I    C 
Sbjct: 133 VI----SEKGEAKIADFGCAKRVDPVFESPVMGTPAFMAPEVARGEKQGKESDIWAVGCT 192

Query: 154 H--------------------NLFFSVVNQMDLPDIPDELSSEGRDFLSKCIYQNSEQRW 194
                                ++ + V    + P++P  L+ E +DFL KC+ + + +RW
Sbjct: 193 MIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCLLAEEAKDFLEKCLKREANERW 252

[Close]

BLAST of HG10009466 vs. ExPASy Swiss-Prot
Match: Q9CAD5 (Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3702 GN=YDA PE=1 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.8e-15
Identity = 57/216 (26.39%), Postives = 101/216 (46.76%), Query Frame = 0


Query: 38  LVLEYAARGSLTKL-------PETEVKDYIRMIVKGLSYIHEQGYVHGDLKPDNILVFPN 97
           + LEY + GS+ KL        E  +++Y + I+ GL+Y+H +  VH D+K  NILV P+
Sbjct: 477 IYLEYVSGGSIYKLLQEYGQFGENAIRNYTQQILSGLAYLHAKNTVHRDIKGANILVDPH 536

Query: 98  DNGKLKLKIADFGLSKRCDE--------------FEEVVISSDANQ-------------- 157
                ++K+ADFG++K                    EV+ +S+ +               
Sbjct: 537 G----RVKVADFGMAKHITAQSGPLSFKGSPYWMAPEVIKNSNGSNLAVDIWSLGCTVLE 596

Query: 158 ------DLEEHQNICHHNLFFSVVNQMDLPDIPDELSSEGRDFLSKCIYQNSEQRWTANM 213
                    +++ +      F + N  +LPDIPD LS EG+DF+ KC+ +N   R TA  
Sbjct: 597 MATTKPPWSQYEGV---PAMFKIGNSKELPDIPDHLSEEGKDFVRKCLQRNPANRPTAAQ 656

[Close]

BLAST of HG10009466 vs. ExPASy Swiss-Prot
Match: Q40541 (Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097 GN=NPK1 PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.4e-15
Identity = 66/230 (28.70%), Postives = 107/230 (46.52%), Query Frame = 0


Query: 1   MTEETILLNFKGCPEIVQSLESGICFEGGSLDSVSYNLVLEYAARGSLTKL-------PE 60
           + EE  LL     P IV+ L  G   E GSL     N++LE+   GS++ L       PE
Sbjct: 130 LEEEVNLLKNLSHPNIVRYL--GTAREAGSL-----NILLEFVPGGSISSLLGKFGSFPE 189

Query: 61  TEVKDYIRMIVKGLSYIHEQGYVHGDLKPDNILVFPNDNGKLKLKIADFGLSKRCDEF-- 120
           + ++ Y + ++ GL Y+H+ G +H D+K  NILV    + K  +K+ADFG SK+  E   
Sbjct: 190 SVIRMYTKQLLLGLEYLHKNGIMHRDIKGANILV----DNKGCIKLADFGASKKVVELAT 249

Query: 121 ---------------EEVVISS--DANQDL------------------EEHQNICHHNLF 180
                           EV++ +    + D+                  +++Q +      
Sbjct: 250 MTGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTIIEMATGKPPWSQQYQEVA---AL 309

Query: 181 FSVVNQMDLPDIPDELSSEGRDFLSKCIYQNSEQRWTANMLLKHPYLKEE 187
           F +      P IP+ LS+E +DFL KC+ +    R +A+ LL+HP++  E
Sbjct: 310 FHIGTTKSHPPIPEHLSAESKDFLLKCLQKEPHLRHSASNLLQHPFVTAE 345

[Close]

BLAST of HG10009466 vs. ExPASy TrEMBL
Match: A0A0A0K835 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G407720 PE=4 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 2.7e-38
Identity = 105/238 (44.12%), Postives = 133/238 (55.88%), Query Frame = 0


Query: 3   EETILLNFKGCPEIVQSLESGICFEGGSLDSVS-YNLVLEYAARGSL-------TKLPET 62
           EE +L +F  CPEIVQ L S +   G  LD    YNL LEYAA G+L        KLPE 
Sbjct: 53  EEHVLKHFTDCPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPED 112

Query: 63  EVKDYIRMIVKGLSYIHEQGYVHGDLKPDNILVFPNDNGKLKLKIADFGLSKR-CDEFE- 122
           EVK Y++MI+KGLS IH +G+VH DLKPDNIL FP  +GK+KLKIADFGL++R C   E 
Sbjct: 113 EVKKYLQMILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGED 172

Query: 123 -------------------------EVVISSDAN--QDL-------------EEHQNIC- 182
                                    E ++ S+ N   D+             E   N C 
Sbjct: 173 DQEDRGSKYYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCK 232

Query: 183 -HHNLFFSVVNQMDLPDIPDELSSEGRDFLSKCIYQNSEQRWTANMLLKHPYLKEECK 189
            +  L   ++   ++P IP+ELS +G+DFL KC  +N EQRWTA+MLL+HPYL EE K
Sbjct: 233 SYEELITKLLISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENK 290

[Close]

BLAST of HG10009466 vs. ExPASy TrEMBL
Match: A0A6J1D9A6 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111018431 PE=4 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 7.8e-38
Identity = 97/226 (42.92%), Postives = 128/226 (56.64%), Query Frame = 0


Query: 1   MTEETILLNFKGCPEIVQSLESGICFEGGSLDSVSYNLVLEYAARGSLT-------KLPE 60
           + E+ IL +F G PEIVQ     +  EG S DS  Y+LVLEYAA G+L        KL E
Sbjct: 45  LNEKRILKHFVGFPEIVQCFGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRKQKKLSE 104

Query: 61  TEVKDYIRMIVKGLSYIHEQGYVHGDLKPDNILVFPNDNGKLKLKIADFGLSKRCDEFEE 120
             VK+Y++MI++GLS IH+ G+VH DLKP NIL FP  +GK+KLKIADFGLS+RC++  E
Sbjct: 105 NTVKEYLKMILRGLSCIHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRE 164

Query: 121 V----------------VISSDANQDLEEHQNIC-----------------HHNLFFSVV 180
                             I  + N  L+     C                    L   + 
Sbjct: 165 CNFKLKFRGTPMYMSPESIYGEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLR 224

Query: 181 NQMDLPDIPDELSSEGRDFLSKCIYQNSEQRWTANMLLKHPYLKEE 187
             M+ P IP+ELS EG+DFL KC  +N ++RWTA+MLL+HPYL++E
Sbjct: 225 VNMETPKIPEELSKEGKDFLEKCFDRNPKRRWTADMLLQHPYLEQE 270

[Close]

BLAST of HG10009466 vs. ExPASy TrEMBL
Match: A0A6J1DHA9 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111020049 PE=4 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 6.6e-37
Identity = 102/234 (43.59%), Postives = 134/234 (57.26%), Query Frame = 0


Query: 3   EETILLNFKGCPEIVQSLESGICFEGGSLDSVSYNLVLEYAARGSLTK-------LPETE 62
           E+ IL +F GC EI+Q +   I  E  SL    YNL+LEYAA G+L         LPE E
Sbjct: 58  EKDILQHFVGCSEIIQCIGDEITLEESSL---CYNLILEYAAGGTLENLIKKQKMLPENE 117

Query: 63  VKDYIRMIVKGLSYIHEQGYVHGDLKPDNILVFPNDNGKLKLKIADFGLSKRC-DEFEEV 122
           VK+Y++MI++GLS IH++G+VHGDLKP NIL FP  +GK+KLKIADFGLSKRC  +  EV
Sbjct: 118 VKEYLKMILRGLSCIHDKGFVHGDLKPTNILAFPQSDGKMKLKIADFGLSKRCVHQDHEV 177

Query: 123 VI--------------------------SSDANQDL-------------EEHQNICHHNL 182
           V+                          S     D+             +E    C  + 
Sbjct: 178 VLDTVTPTSKPKFKGTPIYMSPESVAFGSISPPCDIWSLGCILVEMITGKEVWESCETSG 237

Query: 183 FF--SVVNQMDLP-DIPDELSSEGRDFLSKCIYQNSEQRWTANMLLKHPYLKEE 187
            F   +V +M+LP DIP+ELS EGRDFL+ C   + ++RWTA+MLL+HPYL +E
Sbjct: 238 QFIKKLVYEMELPADIPEELSREGRDFLASCFDHDPKRRWTAHMLLQHPYLTQE 288

[Close]

BLAST of HG10009466 vs. ExPASy TrEMBL
Match: A0A5A7TZP4 (Serine/threonine-protein kinase Chk1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001750 PE=4 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 8.7e-37
Identity = 101/236 (42.80%), Postives = 131/236 (55.51%), Query Frame = 0


Query: 3   EETILLNFKGCPEIVQSLESGICFEGGSLDSVS-YNLVLEYAARGSL-------TKLPET 62
           EE +L +   CPEIVQ L S +   G  LD    YNL LEYAA G+L        KLPE 
Sbjct: 52  EEHVLKHLTDCPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPED 111

Query: 63  EVKDYIRMIVKGLSYIHEQGYVHGDLKPDNILVFPNDNGKLKLKIADFGLSKR------- 122
           EVK Y++MI+KGLS IH +G+VH DLKPDNIL FP  +GK+KLKIADFGL++R       
Sbjct: 112 EVKKYLQMILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERFKHGEDD 171

Query: 123 -----CDEF--------------EEVVISS-DANQDL-------------EEHQNIC--H 182
                C+ +              E +V+   D   D+             E   N C  +
Sbjct: 172 QEDKGCEHYGALRVRATHRYMSPESIVLKEIDGLHDIWSLGCVLVQMVSGERVWNDCKNY 231

Query: 183 HNLFFSVVNQMDLPDIPDELSSEGRDFLSKCIYQNSEQRWTANMLLKHPYLKEECK 189
             L   ++N  ++P IP ELS +G DFL KC  ++ +QRWTA+MLL+HPYL E+ K
Sbjct: 232 EELMTKLLNSKEIPIIPKELSKQGIDFLEKCFVRDYKQRWTADMLLQHPYLNEQNK 287

[Close]

BLAST of HG10009466 vs. ExPASy TrEMBL
Match: A0A6J1DKV0 (mitogen-activated protein kinase kinase kinase YODA-like OS=Momordica charantia OX=3673 GN=LOC111021457 PE=4 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 3.3e-36
Identity = 97/236 (41.10%), Postives = 128/236 (54.24%), Query Frame = 0


Query: 1   MTEETILLNFKGCPEIVQSLESG-ICFEGGSLDSVSYNLVLEYAARGSLT-------KLP 60
           + E+ IL  F GCPEIVQ      I  EG S D  S+ L++EYAA GSL        KL 
Sbjct: 50  LREKCILECFIGCPEIVQCYGGEIIIMEGDSFDLASFFLIMEYAAGGSLADLIKKRKKLA 109

Query: 61  ETEVKDYIRMIVKGLSYIHEQGYVHGDLKPDNILVFPNDNGKLKLKIADFGLSKRCDEFE 120
           E EVKDY+RMI++G++ IH++G+VH DLKP NIL FP  +GK+KLKI DFGL++R  E E
Sbjct: 110 ENEVKDYLRMILRGVACIHDKGFVHADLKPGNILAFPQPDGKMKLKITDFGLTRRSCEEE 169

Query: 121 EVV--------------------ISSDANQDLEEHQNIC----------------HHNLF 180
           E                      +       L+     C                   L 
Sbjct: 170 EAYDDYCVERRFRGTPIYMSPESVYGQVKAPLDIWSVGCIAVEIVSGKPVWDGCPDQKLM 229

Query: 181 FSVVNQMDLPDIPDELSSEGRDFLSKCIYQNSEQRWTANMLLKHPYLKEECKVDEL 193
             +V++M++P IP+E S EG+DF+ +C     E+RWTA MLL+H YLKEE K DE+
Sbjct: 230 LKLVDKMEIPKIPEEFSEEGKDFVGRCFDLEPERRWTAEMLLQHSYLKEENK-DEI 284

[Close]

BLAST of HG10009466 vs. TAIR 10
Match: AT5G27510.1 (Protein kinase superfamily protein )

HSP 1 Score: 116.3 bits (290), Expect = 3.1e-26
Identity = 80/244 (32.79%), Postives = 121/244 (49.59%), Query Frame = 0


Query: 1   MTEETILLNFKGCPEIVQ----SLESGICFEGGSLDSVSYNLVLEYAARGSLT------- 60
           + E  IL   KGCP I+Q     LE G   +G  +    Y L+LEYA+ GSL+       
Sbjct: 46  LKEFHILSELKGCPRIIQCFGNDLEEGFDDKGNRV----YKLLLEYASEGSLSDFMNNCV 105

Query: 61  --KLPETEVKDYIRMIVKGLSYIHEQGYVHGDLKPDNILVFPNDNGKLKLKIADFGLSKR 120
             KLP+  ++D+ RMI++GL  IH  GYVH DLKP+N+LVFP  +   ++KI+DFGLS +
Sbjct: 106 DRKLPDLMIRDFTRMILQGLVSIHSHGYVHCDLKPENVLVFPCGD-SYEVKISDFGLSLQ 165

Query: 121 CDEFE-----EVVISSDANQDLEE--HQNICHHNL------------------------- 180
             E       E       N    E  H  + +  L                         
Sbjct: 166 VGEVPDHWKIEYPFVGTLNYMPPESLHDGVANKTLDLWSLGCLVLEMYVCKKPWIGFIPE 225

Query: 181 -FFSVVNQMDLPDIPDELSSEGRDFLSKCIYQNSEQRWTANMLLKHPYLKEECKVDELLS 199
            F  +++  + P+IP+ L  + R F+ KC  +N ++R TA+ LL H +L++E    +++S
Sbjct: 226 DFVYILSNGNPPEIPESLPCDARAFIQKCFSRNPKERGTASELLSHRFLRQEKSKLKMIS 284

[Close]

BLAST of HG10009466 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 112.5 bits (280), Expect = 4.4e-25
Identity = 81/223 (36.32%), Postives = 109/223 (48.88%), Query Frame = 0


Query: 3   EETILLNFKGCPEIVQSLESGICFEGGSLDSVSYNLVLEYAARGSLT--------KLPET 62
           E  +L +   C EIV+    G   E G      YNL LEYA+ GSL          LPE 
Sbjct: 50  ERDVLDDLGDCSEIVRCFGEGRTVENG---EEIYNLFLEYASGGSLADRIKSSGEALPEF 109

Query: 63  EVKDYIRMIVKGLSYIHEQGYVHGDLKPDNILVFPNDNGKLKLKIADFGLSKR-----C- 122
           EV+ + R IVKGL +IH  G+ H D+K +N+LVF    G   +KI+DFGL+KR     C 
Sbjct: 110 EVRRFTRSIVKGLCHIHGNGFTHCDIKLENVLVF----GDGDVKISDFGLAKRRSGEVCV 169

Query: 123 ----------------DEFE----------EVVISSDANQDLEEHQNICHH--NLFFSVV 182
                            EFE           VV  S           + ++  +L   + 
Sbjct: 170 EIRGTPLYMAPESVNHGEFESPADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLLVRIG 229

Query: 183 NQMDLPDIPDELSSEGRDFLSKCIYQNSEQRWTANMLLKHPYL 184
           +  ++P IP ELS EG+DF+SKC  +N+ +RWTA MLL HP+L
Sbjct: 230 SGDEVPRIPVELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFL 265

[Close]

BLAST of HG10009466 vs. TAIR 10
Match: AT2G34290.1 (Protein kinase superfamily protein )

HSP 1 Score: 103.2 bits (256), Expect = 2.7e-22
Identity = 56/114 (49.12%), Postives = 70/114 (61.40%), Query Frame = 0


Query: 3   EETILLNFKGCPEIVQSLESGICFEGGSLDSVSYNLVLEYAARGSLT---------KLPE 62
           E  IL  FKGC  IVQ   SG+         V Y + +EYAA GSL+         KLP+
Sbjct: 45  EFQILSKFKGCSRIVQCYGSGVEQRLNDKGYVEYTIPMEYAAGGSLSDFMDRFNDKKLPD 104

Query: 63  TEVKDYIRMIVKGLSYIHEQGYVHGDLKPDNILVFPNDNGKLKLKIADFGLSKR 108
             ++ + RM+++GL+ IH  GYVH DLKP+NILVFP     LKLKI+DFGLSKR
Sbjct: 105 PMIRKFTRMLLEGLATIHRHGYVHCDLKPENILVFPGSVCDLKLKISDFGLSKR 158

[Close]

BLAST of HG10009466 vs. TAIR 10
Match: AT2G42550.1 (Protein kinase superfamily protein )

HSP 1 Score: 102.4 bits (254), Expect = 4.6e-22
Identity = 79/227 (34.80%), Postives = 108/227 (47.58%), Query Frame = 0


Query: 3   EETILLNFKGCPEIVQSLESGICFEGGSLDSVS-YNLVLEYAARGSL---------TKLP 62
           E  IL   +GC  IVQ   +    E   +     Y +V+EYAA GSL          KLP
Sbjct: 52  EIQILSKLEGCRRIVQCYGNYTLEEDFDVGGFRVYKMVMEYAAAGSLFSFMDSYKDRKLP 111

Query: 63  ETEVKDYIRMIVKGLSYIHEQGYVHGDLKPDNILVFPNDNGKLKLKIADFGLSKRCDEFE 122
           ET +KD+ RMI++GL  +H  GYVH DLKPDN+LVFP      +LKI+DFG S++  E+ 
Sbjct: 112 ETMIKDFTRMILQGLVSVHRLGYVHCDLKPDNLLVFP-CRQSYELKISDFGSSRKVGEYS 171

Query: 123 ------------------EVVISSDANQDLEEHQNIC------HHNLFFSVVNQMDL--- 182
                             E V S  A + L+     C         + +S V   DL   
Sbjct: 172 DCWDVDLPFVGTPVYMSPESVRSGVAEKALDLWSLGCIVLEMYTGVIPWSEVEFEDLAPA 231

Query: 183 ------PDIPDELSSEGRDFLSKCIYQNSEQRWTANMLLKHPYLKEE 187
                 P+IP  L  + R FL  C  +N ++R +A+ LL H +L+ E
Sbjct: 232 LSKGKAPEIPKSLPCDARKFLETCFSRNPKERGSASDLLSHQFLRGE 277

[Close]

BLAST of HG10009466 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 102.4 bits (254), Expect = 4.6e-22
Identity = 75/239 (31.38%), Postives = 111/239 (46.44%), Query Frame = 0


Query: 3   EETILLNF-KGCPEIVQSLESGICFEGGSLDSVSYNLVLEYAARGSLTK---------LP 62
           E+++L N    C EIV+        E G      +NL LEYA+RGSL           +P
Sbjct: 50  EKSVLDNLGDDCNEIVRCFGEDRTVENG---EEMHNLFLEYASRGSLESYLKKLAGEGVP 109

Query: 63  ETEVKDYIRMIVKGLSYIHEQGYVHGDLKPDNILVFPNDNGKLKLKIADFGLSKRCDEFE 122
           E+ V+ +   +++GL +IH  G+ H DLK  NIL+F    G   +KIADFGL+KR  +  
Sbjct: 110 ESTVRRHTGSVLRGLRHIHANGFAHCDLKLGNILLF----GDGAVKIADFGLAKRIGDLT 169

Query: 123 EV--------------------------------------VISSDANQDLEEHQNICHHN 182
            +                                      + S      L+E  N    +
Sbjct: 170 ALNYGVQIRGTPLYMAPESVNDNEYGSEGDVWALGCVVVEMFSGKTAWSLKEGSNFM--S 229

Query: 183 LFFSVVNQMDLPDIPDELSSEGRDFLSKCIYQNSEQRWTANMLLKHPYLKEECKVDELL 194
           L   +    ++P IP+ELS +GRDFLSKC  ++ ++RWTA MLL HP++  +   D L+
Sbjct: 230 LLLRIGVGDEVPMIPEELSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFVTVDVDHDVLV 279

The following BLAST results are available for this feature:

BLAST of HG10009466 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896352.1	1.3e-71	59.46	mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida]	[more]
XP_038896342.1	5.2e-68	58.20	mitogen-activated protein kinase kinase kinase 3-like [Benincasa hispida]	[more]
XP_038896344.1	6.7e-68	59.36	mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida]	[more]
XP_038896343.1	1.1e-54	56.83	mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida]	[more]
XP_038896328.1	1.2e-43	71.65	mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida]	[more]

BLAST of HG10009466 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SND6	2.4e-20	30.77	Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370...	[more]
F4HRJ4	6.0e-19	29.96	Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702...	[more]
O80888	1.0e-18	31.84	Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370...	[more]
Q9CAD5	1.8e-15	26.39	Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3...	[more]
Q40541	2.4e-15	28.70	Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097...	[more]

BLAST of HG10009466 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K835	2.7e-38	44.12	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G407...	[more]
A0A6J1D9A6	7.8e-38	42.92	mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX...	[more]
A0A6J1DHA9	6.6e-37	43.59	mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX...	[more]
A0A5A7TZP4	8.7e-37	42.80	Serine/threonine-protein kinase Chk1-like OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A6J1DKV0	3.3e-36	41.10	mitogen-activated protein kinase kinase kinase YODA-like OS=Momordica charantia ...	[more]

BLAST of HG10009466 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G27510.1	3.1e-26	32.79	Protein kinase superfamily protein	[more]
AT4G36950.1	4.4e-25	36.32	mitogen-activated protein kinase kinase kinase 21	[more]
AT2G34290.1	2.7e-22	49.12	Protein kinase superfamily protein	[more]
AT2G42550.1	4.6e-22	34.80	Protein kinase superfamily protein	[more]
AT5G67080.1	4.6e-22	31.38	mitogen-activated protein kinase kinase kinase 19	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 1..183 e-value: 4.5E-10 score: 9.5
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 3..110 e-value: 7.2E-19 score: 68.2
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 1..218 score: 16.329729
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 1..120 e-value: 1.5E-21 score: 78.8
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 121..200 e-value: 3.9E-7 score: 31.5
None	No IPR available	PANTHER	PTHR48011:SF51	PROTEIN KINASE SUPERFAMILY PROTEIN	coord: 2..110
None	No IPR available	PANTHER	PTHR48011:SF51	PROTEIN KINASE SUPERFAMILY PROTEIN	coord: 133..191
None	No IPR available	PANTHER	PTHR48011	CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED	coord: 2..110
None	No IPR available	PANTHER	PTHR48011	CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED	coord: 133..191
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 75..87
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 3..209

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10009466.1	HG10009466.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity