HG10009458 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10009458
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Cytochrome p450 76c2
Location	Chr06: 6160161 .. 6161051 (+)
RNA-Seq Expression	HG10009458
Synteny	HG10009458

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGATTCCTTCATTTTTCTTTCTTCTTCCTTCTCTTCTTCTTCTTCTACCTCCAATTTTCATTATTCTCAAACACATCAAATCTCCATCTTCAAAACAACTCCCACTTCCTCCAGGTCCAAGGCCATGGCCAATCTTAGGAAATCTCCTTCAGATTGGTCAAAATGCTCACATTTCCATGACTCAATTTGCCAAGATCCATGGCCCTCTAATCTCCCTCAAGCTCGGAGCTCAGTGCCTCGTCGTCGCTTCTTCTCCGGCTGCCGCCACCGCAATTCTCAAGACGCAAGACCGCCTTCTCTCTGCTCGATACATCTTCCAAATGACCCCTGATAGAGCTCTCCATGATCGCTGTTCTCTTGTTTTCTCCCCTGAATGTGGCGATCTTTGGAAGAATTTGAGGTCCATTTGCAAAGCCAATTTGTTTACAGCCAAAGCAATTGAATCACAAGCTTGTTTGAGAGAGAAGAAAATGAAGGACTTAGTGGAATTTTTAGAGTCAAAACAGGGATCTGTTGTGGAGATAAAAGATTTTGTGTTTACCTCTGTTTTTAATACACTTTCTAACTTGATTTTCTCCATGGATTTGCTTGGTTTTGTTGGTGATGGATTTAATGGGATTAAAGGGCCTTTCAAGAAAATGATGGATTTGGGTTTGACCCCAAATTTAGCTGATTTCTACCCGTTGTTGAGAAGCTTTGATCTTCAAGGTCTTCGAAAGAAAGCTGCCATTTACAAGAATCAAGTTGATTCTTTGTGGGGAGGTCTTATTGAAGAAAGAAGAGAAATCCATAGTCAACAGGGTTCAGCTTCAAGTGACTTCTTGGATGTCTTGATTCAAAGTGGATTCACTGATCAACAAATTAATGCCTTGATTGTTGTAGTATAA

mRNA sequence

ATGGTGATTCCTTCATTTTTCTTTCTTCTTCCTTCTCTTCTTCTTCTTCTACCTCCAATTTTCATTATTCTCAAACACATCAAATCTCCATCTTCAAAACAACTCCCACTTCCTCCAGGTCCAAGGCCATGGCCAATCTTAGGAAATCTCCTTCAGATTGGTCAAAATGCTCACATTTCCATGACTCAATTTGCCAAGATCCATGGCCCTCTAATCTCCCTCAAGCTCGGAGCTCAGTGCCTCGTCGTCGCTTCTTCTCCGGCTGCCGCCACCGCAATTCTCAAGACGCAAGACCGCCTTCTCTCTGCTCGATACATCTTCCAAATGACCCCTGATAGAGCTCTCCATGATCGCTGTTCTCTTGTTTTCTCCCCTGAATGTGGCGATCTTTGGAAGAATTTGAGGTCCATTTGCAAAGCCAATTTGTTTACAGCCAAAGCAATTGAATCACAAGCTTGTTTGAGAGAGAAGAAAATGAAGGACTTAGTGGAATTTTTAGAGTCAAAACAGGGATCTGTTGTGGAGATAAAAGATTTTGTGTTTACCTCTGTTTTTAATACACTTTCTAACTTGATTTTCTCCATGGATTTGCTTGGTTTTGTTGGTGATGGATTTAATGGGATTAAAGGGCCTTTCAAGAAAATGATGGATTTGGGTTTGACCCCAAATTTAGCTGATTTCTACCCGTTGTTGAGAAGCTTTGATCTTCAAGGTCTTCGAAAGAAAGCTGCCATTTACAAGAATCAAGTTGATTCTTTGTGGGGAGGTCTTATTGAAGAAAGAAGAGAAATCCATAGTCAACAGGGTTCAGCTTCAAGTGACTTCTTGGATGTCTTGATTCAAAGTGGATTCACTGATCAACAAATTAATGCCTTGATTGTTGTAGTATAA

Coding sequence (CDS)

ATGGTGATTCCTTCATTTTTCTTTCTTCTTCCTTCTCTTCTTCTTCTTCTACCTCCAATTTTCATTATTCTCAAACACATCAAATCTCCATCTTCAAAACAACTCCCACTTCCTCCAGGTCCAAGGCCATGGCCAATCTTAGGAAATCTCCTTCAGATTGGTCAAAATGCTCACATTTCCATGACTCAATTTGCCAAGATCCATGGCCCTCTAATCTCCCTCAAGCTCGGAGCTCAGTGCCTCGTCGTCGCTTCTTCTCCGGCTGCCGCCACCGCAATTCTCAAGACGCAAGACCGCCTTCTCTCTGCTCGATACATCTTCCAAATGACCCCTGATAGAGCTCTCCATGATCGCTGTTCTCTTGTTTTCTCCCCTGAATGTGGCGATCTTTGGAAGAATTTGAGGTCCATTTGCAAAGCCAATTTGTTTACAGCCAAAGCAATTGAATCACAAGCTTGTTTGAGAGAGAAGAAAATGAAGGACTTAGTGGAATTTTTAGAGTCAAAACAGGGATCTGTTGTGGAGATAAAAGATTTTGTGTTTACCTCTGTTTTTAATACACTTTCTAACTTGATTTTCTCCATGGATTTGCTTGGTTTTGTTGGTGATGGATTTAATGGGATTAAAGGGCCTTTCAAGAAAATGATGGATTTGGGTTTGACCCCAAATTTAGCTGATTTCTACCCGTTGTTGAGAAGCTTTGATCTTCAAGGTCTTCGAAAGAAAGCTGCCATTTACAAGAATCAAGTTGATTCTTTGTGGGGAGGTCTTATTGAAGAAAGAAGAGAAATCCATAGTCAACAGGGTTCAGCTTCAAGTGACTTCTTGGATGTCTTGATTCAAAGTGGATTCACTGATCAACAAATTAATGCCTTGATTGTTGTAGTATAA

Protein sequence

MVIPSFFFLLPSLLLLLPPIFIILKHIKSPSSKQLPLPPGPRPWPILGNLLQIGQNAHISMTQFAKIHGPLISLKLGAQCLVVASSPAAATAILKTQDRLLSARYIFQMTPDRALHDRCSLVFSPECGDLWKNLRSICKANLFTAKAIESQACLREKKMKDLVEFLESKQGSVVEIKDFVFTSVFNTLSNLIFSMDLLGFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQGLRKKAAIYKNQVDSLWGGLIEERREIHSQQGSASSDFLDVLIQSGFTDQQINALIVVV

Homology

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BLAST of HG10009458 vs. NCBI nr
Match: XP_038906737.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Benincasa hispida])

HSP 1 Score: 516.5 bits (1329), Expect = 1.5e-142
Identity = 265/294 (90.14%), Postives = 277/294 (94.22%), Query Frame = 0


Query: 1   MVIPSFFFLLPSLLLLLPPIFIILKHIKSPSSKQLPLPPGPRPWPILGNLLQIGQNAHIS 60
           MVIPSF FLLPSL+LLLPPIFI LKH+KS S KQLPLPPGP+PWPILGNLLQIGQNAHIS
Sbjct: 1   MVIPSFTFLLPSLVLLLPPIFIFLKHVKSRSPKQLPLPPGPKPWPILGNLLQIGQNAHIS 60

Query: 61  MTQFAKIHGPLISLKLGAQCLVVASSPAAATAILKTQDRLLSARYIFQMTPDRALHDRCS 120
           MTQFA I+GPLISLKLGAQ LVVASSPAAATAILKTQD LLSARYI QMTPDRAL+D CS
Sbjct: 61  MTQFANIYGPLISLKLGAQHLVVASSPAAATAILKTQDSLLSARYILQMTPDRALNDHCS 120

Query: 121 LVFSPECGDLWKNLRSICKANLFTAKAIESQACLREKKMKDLVEFLESKQGSVVEIKDFV 180
           LVFSPECG  WKNLRSICKAN+FTAKAIESQACLRE+KMKDLVEFL+SKQGSVVEIKD V
Sbjct: 121 LVFSPECGHRWKNLRSICKANMFTAKAIESQACLREQKMKDLVEFLDSKQGSVVEIKDLV 180

Query: 181 FTSVFNTLSNLIFSMDLLGFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQGLR 240
           FTSVFNTLSNLIFS DLLGFVGDGFNGIKGPFK +MDLGLTPNLADFYPLLRSFDLQGLR
Sbjct: 181 FTSVFNTLSNLIFSRDLLGFVGDGFNGIKGPFKNLMDLGLTPNLADFYPLLRSFDLQGLR 240

Query: 241 KKAAIYKNQVDSLWGGLIEERREIHSQQGSASSDFLDVLIQSGFTDQQINALIV 295
           KKAAIYKNQVDSLWG LI+ERREIH QQGS+SSDFLDVLIQSGFTDQ+IN LIV
Sbjct: 241 KKAAIYKNQVDSLWGILIKERREIHGQQGSSSSDFLDVLIQSGFTDQEINTLIV 294

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BLAST of HG10009458 vs. NCBI nr
Match: TYK00293.1 (putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo var. makuwa])

HSP 1 Score: 499.2 bits (1284), Expect = 2.4e-137
Identity = 261/295 (88.47%), Postives = 276/295 (93.56%), Query Frame = 0


Query: 1   MVIPSFFFLLPSLLLLLPPIFIILKHIKSPSSKQLPLPPGPRPWPILGNLLQIGQNAHIS 60
           M+IPSF  LLPSLLLLL PIFIILKHIKSPSSK LPLPPGP PWPILGNLLQIGQNAHIS
Sbjct: 1   MLIPSFSILLPSLLLLL-PIFIILKHIKSPSSK-LPLPPGPNPWPILGNLLQIGQNAHIS 60

Query: 61  MTQFAKIHGPLISLKLGAQCLVVASSPAAATAILKTQDRLLSARYIFQMTPDRALHDRCS 120
           +TQFA I+GPLISLKLGAQ L+VASSPAAATA+LKTQDRLLSARYIFQMTPDRALHD+ S
Sbjct: 61  ITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHS 120

Query: 121 LVFSPECGDLWKNLRSICKANLFTAKAIESQACLREKKMKDLVEFLESKQGSVVEIKDFV 180
           LVFSPECGD WKNLRS+CK NLFTAKAIESQA LREKKMK+LV+FLESKQGSVVE+KDFV
Sbjct: 121 LVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFV 180

Query: 181 FTSVFNTLSNLIFSMDLLGFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQGLR 240
           FTSVFNTLSNLIFSMDLL FVGDGF GIKGPFKKMMDLGLTPNLADFYPLLR FDLQGL+
Sbjct: 181 FTSVFNTLSNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLK 240

Query: 241 KKAAIYKNQVDSLWGGLIEERREIHSQQGSASSDFLDVLIQSGFTDQQINALIVV 296
            KA IYKN+VDSLWG LI+ERREIH QQGSAS+DFLDVLIQSGF DQQINALI+V
Sbjct: 241 NKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIV 293

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BLAST of HG10009458 vs. NCBI nr
Match: XP_008467223.1 (PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo])

HSP 1 Score: 498.0 bits (1281), Expect = 5.4e-137
Identity = 260/294 (88.44%), Postives = 275/294 (93.54%), Query Frame = 0


Query: 1   MVIPSFFFLLPSLLLLLPPIFIILKHIKSPSSKQLPLPPGPRPWPILGNLLQIGQNAHIS 60
           M+IPSF  LLPSLLLLL PIFIILKHIKSPSSK LPLPPGP PWPILGNLLQIGQNAHIS
Sbjct: 1   MLIPSFSILLPSLLLLL-PIFIILKHIKSPSSK-LPLPPGPNPWPILGNLLQIGQNAHIS 60

Query: 61  MTQFAKIHGPLISLKLGAQCLVVASSPAAATAILKTQDRLLSARYIFQMTPDRALHDRCS 120
           +TQFA I+GPLISLKLGAQ L+VASSPAAATA+LKTQDRLLSARYIFQMTPDRALHD+ S
Sbjct: 61  ITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHS 120

Query: 121 LVFSPECGDLWKNLRSICKANLFTAKAIESQACLREKKMKDLVEFLESKQGSVVEIKDFV 180
           LVFSPECGD WKNLRS+CK NLFTAKAIESQA LREKKMK+LV+FLESKQGSVVE+KDFV
Sbjct: 121 LVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFV 180

Query: 181 FTSVFNTLSNLIFSMDLLGFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQGLR 240
           FTSVFNTLSNLIFSMDLL FVGDGF GIKGPFKKMMDLGLTPNLADFYPLLR FDLQGL+
Sbjct: 181 FTSVFNTLSNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLK 240

Query: 241 KKAAIYKNQVDSLWGGLIEERREIHSQQGSASSDFLDVLIQSGFTDQQINALIV 295
            KA IYKN+VDSLWG LI+ERREIH QQGSAS+DFLDVLIQSGF DQQINALI+
Sbjct: 241 NKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALII 292

[Close]

BLAST of HG10009458 vs. NCBI nr
Match: KAA0055170.1 (putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo var. makuwa])

HSP 1 Score: 497.3 bits (1279), Expect = 9.3e-137
Identity = 259/294 (88.10%), Postives = 275/294 (93.54%), Query Frame = 0


Query: 1   MVIPSFFFLLPSLLLLLPPIFIILKHIKSPSSKQLPLPPGPRPWPILGNLLQIGQNAHIS 60
           M+IPSF  LLPSLLLLL PIFIILKHIKSPSSK LPLPPGP PWPILGNLLQIGQNAHIS
Sbjct: 1   MLIPSFSILLPSLLLLL-PIFIILKHIKSPSSK-LPLPPGPNPWPILGNLLQIGQNAHIS 60

Query: 61  MTQFAKIHGPLISLKLGAQCLVVASSPAAATAILKTQDRLLSARYIFQMTPDRALHDRCS 120
           +TQFA I+GPLISLKLGAQ L+VASSPAAATA+LKTQDRLLSARYIFQMTPDRALHD+ S
Sbjct: 61  ITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHS 120

Query: 121 LVFSPECGDLWKNLRSICKANLFTAKAIESQACLREKKMKDLVEFLESKQGSVVEIKDFV 180
           LVFSPECGD WKNLRS+CK NLFTAKAIESQA LREKKMK+LV+FLESKQGSVVE+KDFV
Sbjct: 121 LVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFV 180

Query: 181 FTSVFNTLSNLIFSMDLLGFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQGLR 240
           FTSVFNTLSNLIFSMDL+ FVGDGF GIKGPFKKMMDLGLTPNLADFYPLLR FDLQGL+
Sbjct: 181 FTSVFNTLSNLIFSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLK 240

Query: 241 KKAAIYKNQVDSLWGGLIEERREIHSQQGSASSDFLDVLIQSGFTDQQINALIV 295
            KA IYKN+VDSLWG LI+ERREIH QQGSAS+DFLDVLIQSGF DQQINALI+
Sbjct: 241 NKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALII 292

[Close]

BLAST of HG10009458 vs. NCBI nr
Match: KAE8647950.1 (hypothetical protein Csa_000545 [Cucumis sativus])

HSP 1 Score: 491.5 bits (1264), Expect = 5.1e-135
Identity = 256/293 (87.37%), Postives = 270/293 (92.15%), Query Frame = 0


Query: 1   MVIPSFFFLLPSLLLLLPPIFIILKHIKSPSSKQLPLPPGPRPWPILGNLLQIGQNAHIS 60
           M+IPSF  LLPSLLLL+PPIFIILK IKSPS K+LPLPPGP PWPILGNL QIGQNAHIS
Sbjct: 1   MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS 60

Query: 61  MTQFAKIHGPLISLKLGAQCLVVASSPAAATAILKTQDRLLSARYIFQMTPDRALHDRCS 120
           +TQFA I+GPLISLKLGAQ LVVASSPA ATA+LKT DR LS RYI QMTPDRAL+++ S
Sbjct: 61  ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS 120

Query: 121 LVFSPECGDLWKNLRSICKANLFTAKAIESQACLREKKMKDLVEFLESKQGSVVEIKDFV 180
           LVFSPECGD WKNLRSICKANLFTAKAIESQA LREKKMK+LVEFLESKQGSVV+IKDFV
Sbjct: 121 LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV 180

Query: 181 FTSVFNTLSNLIFSMDLLGFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQGLR 240
           FTSVFNTLSNLIFSMDLL FVGDGFNGIKG FKKMMDLGLTPNLADFYPLLR FDLQGLR
Sbjct: 181 FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR 240

Query: 241 KKAAIYKNQVDSLWGGLIEERREIHSQQGSASSDFLDVLIQSGFTDQQINALI 294
           KKAAIY N+VDSLWG LI+ERREIH QQGSASSDFLDVLIQSGF+DQQIN LI
Sbjct: 241 KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLI 293

[Close]

BLAST of HG10009458 vs. ExPASy Swiss-Prot
Match: O64900 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3467 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 2.2e-56
Identity = 119/282 (42.20%), Postives = 181/282 (64.18%), Query Frame = 0


Query: 16  LLPPIFIILKHIKSPSSKQLPLPPGPRPWPILGNLLQIGQNAHISMTQFAKIHGPLISLK 75
           L+  I   + ++   SS    LPPGP+PWPI+GNLLQ+G+  H    + A+ +G + +LK
Sbjct: 8   LIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLK 67

Query: 76  LGAQCLVVASSPAAATAILKTQDRLLSARYIFQMTPDRALHDRCSLVFSPECGDLWKNLR 135
           +G + +VVAS+ +AA+ ILKT DR+LSARY+FQ    +  H   S+V+S +C + WKNLR
Sbjct: 68  MGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKG-HVENSIVWS-DCTETWKNLR 127

Query: 136 SICKANLFTAKAIESQACLREKKMKDLVEFLESKQGSVVEIKDFVFTSVFNTLSNLIFSM 195
            +C+  LFT K IESQA +REKK +++VE+L  KQG  V+I + +F ++ N   NLIFS 
Sbjct: 128 KVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQ 187

Query: 196 DL--LGFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQGLRKKAAIYKNQVDSL 255
           ++  LG    G +  K    +M++LG + N AD++P+L  FDL G RK+ A     + ++
Sbjct: 188 NIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAI 247

Query: 256 WGGLIEERREIHSQQG-SASSDFLDVLIQSGFTDQQINALIV 295
           WG +++ER+      G  + +DF+DV + SG  D QINAL++
Sbjct: 248 WGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLM 287

[Close]

BLAST of HG10009458 vs. ExPASy Swiss-Prot
Match: Q9SP06 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum OX=3469 GN=CYP80B3 PE=1 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 2.9e-56
Identity = 117/262 (44.66%), Postives = 169/262 (64.50%), Query Frame = 0


Query: 37  LPPGPRPWPILGNLLQIGQNAHISMTQFAKIHGPLISLKLGAQCLVVASSPAAATAILKT 96
           LPPGP+PWPI+GNLLQ+G+  H    Q A+ +G L SLKLG++ +VVAS+P AA+ ILKT
Sbjct: 23  LPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSLKLGSETVVVASTPLAASEILKT 82

Query: 97  QDRLLSARYIFQMTPDRALHDRCSLVFSPECGDLWKNLRSICKANLFTAKAIESQACLRE 156
            DR+LS RY+FQ    +  H   S+V+S EC + WK LR +C+  LFT K IESQA +RE
Sbjct: 83  HDRVLSGRYVFQSFRVKE-HVENSIVWS-ECNETWKKLRKVCRTELFTQKMIESQAEVRE 142

Query: 157 KKMKDLVEFLESKQGSVVEIKDFVFTSVFNTLSNLIFSMDL--LGFVGDGFNGIKGPFKK 216
            K  ++VE+L+   G+ V+I + VF ++ N   NLIFS ++  LG    G   +K    +
Sbjct: 143 SKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWR 202

Query: 217 MMDLGLTPNLADFYPLLRSFDLQGLRKKAAIYKNQVDSLWGGLIEERREIHSQQGSASSD 276
           M++LG + N AD++P L  FDL G  K  A     + S+WG +++ER+       S  +D
Sbjct: 203 MLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKERKIAKQHNNSKKND 262

Query: 277 FLDVLIQSGFTDQQINALIVVV 297
           F+++L+ SG  DQQINAL++ +
Sbjct: 263 FVEILLDSGLDDQQINALLMEI 282

[Close]

BLAST of HG10009458 vs. ExPASy Swiss-Prot
Match: O64899 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica OX=3467 GN=CYP80B1 PE=1 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.1e-55
Identity = 119/286 (41.61%), Postives = 182/286 (63.64%), Query Frame = 0


Query: 12  SLLLLLPPIFIILKHIKSPSSKQLPLPPGPRPWPILGNLLQIGQNAHISMTQFAKIHGPL 71
           S + L+  I   + ++    S    LPPGP+PWPI+GNLLQ+G+  H    + A+ +G +
Sbjct: 3   STVALIAVIISSILYLLFGGSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDI 62

Query: 72  ISLKLGAQCLVVASSPAAATAILKTQDRLLSARYIFQMTPDRALHDRCSLVFSPECGDLW 131
            +LK+G + +VVAS+ +AA+ ILKT DR+LSARY+FQ    +  H   S+V+S +C + W
Sbjct: 63  FTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKG-HVENSIVWS-DCTETW 122

Query: 132 KNLRSICKANLFTAKAIESQACLREKKMKDLVEFLESKQGSVVEIKDFVFTSVFNTLSNL 191
           KNLR +C+  LFT K IESQA +REKK +++VE+L  KQG  V+I + +F ++ N   NL
Sbjct: 123 KNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNL 182

Query: 192 IFSMDL--LGFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQGLRKKAAIYKNQ 251
           IFS ++  LG    G +  K    +M++LG + N AD++P+L  FDL G RK+ A     
Sbjct: 183 IFSQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKG 242

Query: 252 VDSLWGGLIEERREIHSQQG-SASSDFLDVLIQSGFTDQQINALIV 295
           + ++WG +++ER+      G  + +DF+DV + SG  D QINAL++
Sbjct: 243 IYAIWGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLM 286

[Close]

BLAST of HG10009458 vs. ExPASy Swiss-Prot
Match: Q9FXW4 (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3442 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.0e-53
Identity = 130/297 (43.77%), Postives = 184/297 (61.95%), Query Frame = 0


Query: 1   MVIPSFFFLLPSLLLLLPPIFIILKHIKSPSSKQLPLPPGPRPWPILGNLLQIGQNAHIS 60
           + I SF FLL         +F IL+      S+   LPPGPRP PI+GNLLQ+G   H  
Sbjct: 6   IAIVSFSFLL--------FLFFILR-----DSRPKNLPPGPRPSPIVGNLLQLGDKPHAE 65

Query: 61  MTQFAKIHGPLISLKLGAQCLVVASSPAAATAILKTQDRLLSARYIFQMTPDRALHDRCS 120
             + A+ +G L SLKLG+Q +VVASSPAAA  ILKT D++LS RY+FQ    +  H   S
Sbjct: 66  FAKLAQKYGELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKE-HVENS 125

Query: 121 LVFSPECGDLWKNLRSICKANLFTAKAIESQACLREKKMKDLVEFLESKQGSVVEIKDFV 180
           +V+S EC D WK LR +C+  LFT K IESQ+ +RE K +++V+FL  K+G VV+I + V
Sbjct: 126 IVWS-ECNDNWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVV 185

Query: 181 FTSVFNTLSNLIFSMDLLGFVG--DGFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQG 240
           F ++ N   NLIFS D+        G   +K    K++D+G + N AD++P++   DL G
Sbjct: 186 FGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFG 245

Query: 241 LRKKAAIYKNQVDSLWGGLIEERREIHSQQGSAS-SDFLDVLIQSGFTDQQINALIV 295
            R+  A    Q+  +WG +++ERR     +GS S +DF+DVL+ +G  DQ+INAL++
Sbjct: 246 QRRAVAEVLQQIYDVWGVMLKERR---GTKGSESKNDFVDVLLNAGLDDQKINALLM 284

[Close]

BLAST of HG10009458 vs. ExPASy Swiss-Prot
Match: I3V6B1 ((S)-N-methylcoclaurine 3'-hydroxylase-like protein OS=Papaver somniferum OX=3469 GN=CYP80BX PE=2 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 8.6e-53
Identity = 118/282 (41.84%), Postives = 171/282 (60.64%), Query Frame = 0


Query: 16  LLPPIFIILKHIKSPSSKQLPLPPGPRPWPILGNLLQIGQNAHISMTQFAKIHGPLISLK 75
           L+  +F    ++    S    LPPGP+PWPI+GNLLQ+G+  H    Q A+ +G L +LK
Sbjct: 8   LIAIVFTTFLYLIVRESSPNGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFTLK 67

Query: 76  LGAQCLVVASSPAAATAILKTQDRLLSARYIFQMTPDRALHDRCSLVFSPECGDLWKNLR 135
           LG Q +VVAS+P AA+ +LK  DR L  RY+FQ    +  H   S+V++ EC + WK LR
Sbjct: 68  LGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKN-HVENSIVWN-ECNETWKKLR 127

Query: 136 SICKANLFTAKAIESQACLREKKMKDLVEFLESKQGSVVEIKDFVFTSVFNTLSNLIFSM 195
            +C+  LFT K IE+QA +RE K  ++V++L+  +G  V+I + +F ++ N   NLIFS 
Sbjct: 128 KVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVNMFGNLIFSQ 187

Query: 196 DL--LGFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQGLRKKAAIYKNQVDSL 255
           D+  LG    G   +K    +M++LG + N  D+ PLL S DL G RK  A     V  +
Sbjct: 188 DIFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQRKDVADCLQGVYGV 247

Query: 256 WGGLIEERREIHSQ-QGSASSDFLDVLIQSGFTDQQINALIV 295
           WG +++ERR    Q  G   +DF+DVL+ SG  DQQIN+L++
Sbjct: 248 WGAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLL 287

[Close]

BLAST of HG10009458 vs. ExPASy TrEMBL
Match: A0A5D3BKL7 (Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1728G00360 PE=4 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 1.2e-137
Identity = 261/295 (88.47%), Postives = 276/295 (93.56%), Query Frame = 0


Query: 1   MVIPSFFFLLPSLLLLLPPIFIILKHIKSPSSKQLPLPPGPRPWPILGNLLQIGQNAHIS 60
           M+IPSF  LLPSLLLLL PIFIILKHIKSPSSK LPLPPGP PWPILGNLLQIGQNAHIS
Sbjct: 1   MLIPSFSILLPSLLLLL-PIFIILKHIKSPSSK-LPLPPGPNPWPILGNLLQIGQNAHIS 60

Query: 61  MTQFAKIHGPLISLKLGAQCLVVASSPAAATAILKTQDRLLSARYIFQMTPDRALHDRCS 120
           +TQFA I+GPLISLKLGAQ L+VASSPAAATA+LKTQDRLLSARYIFQMTPDRALHD+ S
Sbjct: 61  ITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHS 120

Query: 121 LVFSPECGDLWKNLRSICKANLFTAKAIESQACLREKKMKDLVEFLESKQGSVVEIKDFV 180
           LVFSPECGD WKNLRS+CK NLFTAKAIESQA LREKKMK+LV+FLESKQGSVVE+KDFV
Sbjct: 121 LVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFV 180

Query: 181 FTSVFNTLSNLIFSMDLLGFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQGLR 240
           FTSVFNTLSNLIFSMDLL FVGDGF GIKGPFKKMMDLGLTPNLADFYPLLR FDLQGL+
Sbjct: 181 FTSVFNTLSNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLK 240

Query: 241 KKAAIYKNQVDSLWGGLIEERREIHSQQGSASSDFLDVLIQSGFTDQQINALIVV 296
            KA IYKN+VDSLWG LI+ERREIH QQGSAS+DFLDVLIQSGF DQQINALI+V
Sbjct: 241 NKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIV 293

[Close]

BLAST of HG10009458 vs. ExPASy TrEMBL
Match: A0A1S3CT14 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656 GN=LOC103504626 PE=3 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 2.6e-137
Identity = 260/294 (88.44%), Postives = 275/294 (93.54%), Query Frame = 0


Query: 1   MVIPSFFFLLPSLLLLLPPIFIILKHIKSPSSKQLPLPPGPRPWPILGNLLQIGQNAHIS 60
           M+IPSF  LLPSLLLLL PIFIILKHIKSPSSK LPLPPGP PWPILGNLLQIGQNAHIS
Sbjct: 1   MLIPSFSILLPSLLLLL-PIFIILKHIKSPSSK-LPLPPGPNPWPILGNLLQIGQNAHIS 60

Query: 61  MTQFAKIHGPLISLKLGAQCLVVASSPAAATAILKTQDRLLSARYIFQMTPDRALHDRCS 120
           +TQFA I+GPLISLKLGAQ L+VASSPAAATA+LKTQDRLLSARYIFQMTPDRALHD+ S
Sbjct: 61  ITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHS 120

Query: 121 LVFSPECGDLWKNLRSICKANLFTAKAIESQACLREKKMKDLVEFLESKQGSVVEIKDFV 180
           LVFSPECGD WKNLRS+CK NLFTAKAIESQA LREKKMK+LV+FLESKQGSVVE+KDFV
Sbjct: 121 LVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFV 180

Query: 181 FTSVFNTLSNLIFSMDLLGFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQGLR 240
           FTSVFNTLSNLIFSMDLL FVGDGF GIKGPFKKMMDLGLTPNLADFYPLLR FDLQGL+
Sbjct: 181 FTSVFNTLSNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLK 240

Query: 241 KKAAIYKNQVDSLWGGLIEERREIHSQQGSASSDFLDVLIQSGFTDQQINALIV 295
            KA IYKN+VDSLWG LI+ERREIH QQGSAS+DFLDVLIQSGF DQQINALI+
Sbjct: 241 NKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALII 292

[Close]

BLAST of HG10009458 vs. ExPASy TrEMBL
Match: A0A5A7UIU3 (Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G001030 PE=3 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 4.5e-137
Identity = 259/294 (88.10%), Postives = 275/294 (93.54%), Query Frame = 0


Query: 1   MVIPSFFFLLPSLLLLLPPIFIILKHIKSPSSKQLPLPPGPRPWPILGNLLQIGQNAHIS 60
           M+IPSF  LLPSLLLLL PIFIILKHIKSPSSK LPLPPGP PWPILGNLLQIGQNAHIS
Sbjct: 1   MLIPSFSILLPSLLLLL-PIFIILKHIKSPSSK-LPLPPGPNPWPILGNLLQIGQNAHIS 60

Query: 61  MTQFAKIHGPLISLKLGAQCLVVASSPAAATAILKTQDRLLSARYIFQMTPDRALHDRCS 120
           +TQFA I+GPLISLKLGAQ L+VASSPAAATA+LKTQDRLLSARYIFQMTPDRALHD+ S
Sbjct: 61  ITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHS 120

Query: 121 LVFSPECGDLWKNLRSICKANLFTAKAIESQACLREKKMKDLVEFLESKQGSVVEIKDFV 180
           LVFSPECGD WKNLRS+CK NLFTAKAIESQA LREKKMK+LV+FLESKQGSVVE+KDFV
Sbjct: 121 LVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFV 180

Query: 181 FTSVFNTLSNLIFSMDLLGFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQGLR 240
           FTSVFNTLSNLIFSMDL+ FVGDGF GIKGPFKKMMDLGLTPNLADFYPLLR FDLQGL+
Sbjct: 181 FTSVFNTLSNLIFSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLK 240

Query: 241 KKAAIYKNQVDSLWGGLIEERREIHSQQGSASSDFLDVLIQSGFTDQQINALIV 295
            KA IYKN+VDSLWG LI+ERREIH QQGSAS+DFLDVLIQSGF DQQINALI+
Sbjct: 241 NKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALII 292

[Close]

BLAST of HG10009458 vs. ExPASy TrEMBL
Match: A0A0A0KRT5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G173480 PE=4 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 3.2e-135
Identity = 257/295 (87.12%), Postives = 271/295 (91.86%), Query Frame = 0


Query: 1   MVIPSFFFLLPSLLLLLPPIFIILKHIKSPSSKQLPLPPGPRPWPILGNLLQIGQNAHIS 60
           M+IPSF  LLPSLLLL+PPIFIILK IKSPS K+LPLPPGP PWPILGNL QIGQNAHIS
Sbjct: 1   MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS 60

Query: 61  MTQFAKIHGPLISLKLGAQCLVVASSPAAATAILKTQDRLLSARYIFQMTPDRALHDRCS 120
           +TQFA I+GPLISLKLGAQ LVVASSPA ATA+LKT DR LS RYI QMTPDRAL+++ S
Sbjct: 61  ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS 120

Query: 121 LVFSPECGDLWKNLRSICKANLFTAKAIESQACLREKKMKDLVEFLESKQGSVVEIKDFV 180
           LVFSPECGD WKNLRSICKANLFTAKAIESQA LREKKMK+LVEFLESKQGSVV+IKDFV
Sbjct: 121 LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV 180

Query: 181 FTSVFNTLSNLIFSMDLLGFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQGLR 240
           FTSVFNTLSNLIFSMDLL FVGDGFNGIKG FKKMMDLGLTPNLADFYPLLR FDLQGLR
Sbjct: 181 FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR 240

Query: 241 KKAAIYKNQVDSLWGGLIEERREIHSQQGSASSDFLDVLIQSGFTDQQINALIVV 296
           KKAAIY N+VDSLWG LI+ERREIH QQGSASSDFLDVLIQSGF+DQQIN LI V
Sbjct: 241 KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINV 295

[Close]

BLAST of HG10009458 vs. ExPASy TrEMBL
Match: A0A0A0KPU9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G173490 PE=4 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 1.0e-133
Identity = 250/295 (84.75%), Postives = 271/295 (91.86%), Query Frame = 0


Query: 1   MVIPSFFFLLPSLLLLLPPIFIILKHIKSPSSKQLPLPPGPRPWPILGNLLQIGQNAHIS 60
           M+IPSF  LLPSLLLLLPPIFIILK IKSPSSK+LPLPPGP PWPILGNLLQIGQNAHIS
Sbjct: 11  MLIPSFSILLPSLLLLLPPIFIILKRIKSPSSKKLPLPPGPNPWPILGNLLQIGQNAHIS 70

Query: 61  MTQFAKIHGPLISLKLGAQCLVVASSPAAATAILKTQDRLLSARYIFQMTPDRALHDRCS 120
           +TQFA I+GPLIS KLGAQ LVVASSPAAATA+LKTQD LLSARYIFQ+TPDRALHD+ S
Sbjct: 71  ITQFANIYGPLISFKLGAQRLVVASSPAAATAVLKTQDHLLSARYIFQITPDRALHDQHS 130

Query: 121 LVFSPECGDLWKNLRSICKANLFTAKAIESQACLREKKMKDLVEFLESKQGSVVEIKDFV 180
           LVFS ECGD+WK LRSICK NLFTAKA ESQA LREKKMK+LVEF+ESKQGSVVEIKDFV
Sbjct: 131 LVFSSECGDVWKKLRSICKVNLFTAKAFESQAILREKKMKELVEFVESKQGSVVEIKDFV 190

Query: 181 FTSVFNTLSNLIFSMDLLGFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQGLR 240
           FTS FN LSNLIFSMDLL FVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLR FDLQGL 
Sbjct: 191 FTSAFNILSNLIFSMDLLDFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLI 250

Query: 241 KKAAIYKNQVDSLWGGLIEERREIHSQQGSASSDFLDVLIQSGFTDQQINALIVV 296
           +K  IYKN+V+S+WG LI+ERREI  ++GSAS+DFLDVLIQSGF+DQQI+ALI+V
Sbjct: 251 RKTVIYKNEVNSVWGILIKERREIRCEKGSASNDFLDVLIQSGFSDQQIDALIIV 305

[Close]

BLAST of HG10009458 vs. TAIR 10
Match: AT3G52970.1 (cytochrome P450, family 76, subfamily G, polypeptide 1 )

HSP 1 Score: 143.3 bits (360), Expect = 3.2e-34
Identity = 85/276 (30.80%), Postives = 148/276 (53.62%), Query Frame = 0


Query: 37  LPPGPRPWPILGNLLQI-GQNAHISMTQFAKIHGPLISLKLGAQCLVVASSPAAATAILK 96
           LPPGP PWP++GN+ Q+ G   H S+T+ ++ HGP+++L++G+   VV SS   A  I K
Sbjct: 36  LPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHGPIMTLRIGSMLTVVISSSEVAREIFK 95

Query: 97  TQDRLLSARYIFQMTPDRALHDRCSLVFSPECGDLWKNLRSICKANLFTAKAIESQACLR 156
             D  L+ R I++        D    + + + G  W+ LR +C    F  + +++ + +R
Sbjct: 96  KHDAALAGRKIYEAMKGGKSSD--GSLITAQYGAYWRMLRRLCTTQFFVTRRLDAMSDVR 155

Query: 157 EKKMKDLVEFLE--SKQGS-VVEIKDFVFTSVFNTLSNLIFSMDLLGFVGDGFNGIKGPF 216
            + +  ++ F+E   + G+  +++  + F   FN + NL+FS DLL    D   G +  +
Sbjct: 156 SRCVDQMLRFVEEGGQNGTKTIDVGRYFFLMAFNLIGNLMFSRDLLD--PDSKRGSEFFY 215

Query: 217 K--KMMDLGLTPNLADFYPLLRSFDLQGLRKKAAIYKNQVDSLWGGLIEERREIHSQQGS 276
              K+M+    PN+ADF+PLLR  D QG+R+K   +  +   + G  I ER E+  ++ S
Sbjct: 216 HTGKVMEFAGKPNVADFFPLLRFLDPQGIRRKTQFHVEKAFEIAGEFIRERTEVREREKS 275

Query: 277 --ASSDFLDVLIQ-----------SGFTDQQINALI 294
              + D+LDVL++           S F+ + IN ++
Sbjct: 276 DEKTKDYLDVLLEFRGGDGVDEEPSSFSSRDINVIV 307

[Close]

BLAST of HG10009458 vs. TAIR 10
Match: AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )

HSP 1 Score: 140.6 bits (353), Expect = 2.1e-33
Identity = 95/290 (32.76%), Postives = 154/290 (53.10%), Query Frame = 0


Query: 2   VIPSFFFLLPSLLLLLPPIFIILKHIKSPSSKQLPLPPGPRPWPILGNLLQIGQNAHISM 61
           + P F F+L   +     IF      +S S K +P PPGP   PI+GN+  +G+N H S 
Sbjct: 9   LFPLFCFVLSFFI-----IFFTTTRPRS-SRKVVPSPPGPPRLPIIGNIHLVGRNPHHSF 68

Query: 62  TQFAKIHGPLISLKLGAQCLVVASSPAAATAILKTQDRLLSARYIFQMTPDRAL----HD 121
              +K +GP++SLK G+   VV +SP AA  +L+T D++LS+R     TP  ++    HD
Sbjct: 69  ADLSKTYGPIMSLKFGSLNTVVVTSPEAAREVLRTYDQILSSR-----TPTNSIRSINHD 128

Query: 122 RCSLVFSPECGDLWKNLRSICKANLFTAKAIESQACLREKKMKDLVEFL--ESKQGSVVE 181
           + S+V+ P     W+ LR +    LF+ + IE+   LRE K+K+LV F+   S++   V+
Sbjct: 129 KVSVVWLPPSSSRWRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESSEREEAVD 188

Query: 182 IKDFVFTSVFNTLSNLIFSMDLLGFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRSFD 241
           I    F +  N +SN++FS+DL  +  +     +     +M+    P+ A+F+P L   D
Sbjct: 189 ISRATFITALNIISNILFSVDLGNYDSNKSGVFQDTVIGVMEAVGNPDAANFFPFLGFLD 248

Query: 242 LQGLRKKAAIYKNQVDSLWGGLIEERREIHSQQGSASS-----DFLDVLI 281
           LQG RK       ++  ++ G I+ +    S + + S      DF+DVL+
Sbjct: 249 LQGNRKTLKACSERLFKVFRGFIDAKLAEKSLRDTNSKDVRERDFVDVLL 287

[Close]

BLAST of HG10009458 vs. TAIR 10
Match: AT2G45560.1 (cytochrome P450, family 76, subfamily C, polypeptide 1 )

HSP 1 Score: 139.4 bits (350), Expect = 4.6e-33
Identity = 87/255 (34.12%), Postives = 141/255 (55.29%), Query Frame = 0


Query: 37  LPPGPRPWPILGNLLQIGQNAHISMTQFAKIHGPLISLKLGAQCLVVASSPAAATAILKT 96
           LPPGP   PI+GN+  +G++ H S  + +K +GP++SLKLG+   VV +SP AA  +L+T
Sbjct: 38  LPPGPPRLPIIGNIHLVGKHPHRSFAELSKTYGPVMSLKLGSLNTVVIASPEAAREVLRT 97

Query: 97  QDRLLSARYIFQMTPDRAL----HDRCSLVFSPECGDLWKNLRSICKANLFTAKAIESQA 156
            D++LSAR     +P  A+    H   SLV+ P     W+ LR +    L + + IE+  
Sbjct: 98  HDQILSAR-----SPTNAVRSINHQDASLVWLPSSSARWRLLRRLSVTQLLSPQRIEATK 157

Query: 157 CLREKKMKDLVEFL--ESKQGSVVEIKDFVFTSVFNTLSNLIFSMDLLGF-VGDGFNGIK 216
            LR  K+K+LV F+   S +   V+I    F +  N +SN++FS+DL  +      NG++
Sbjct: 158 ALRMNKVKELVSFISESSDREESVDISRVAFITTLNIISNILFSVDLGSYNAKASINGVQ 217

Query: 217 GPFKKMMDLGLTPNLADFYPLLRSFDLQGLRKKAAIYKNQVDSLWGGLIEERREIHSQQG 276
                +MD   TP+ A+++P LR  DLQG  K   +   ++  ++ G I+ +    S Q 
Sbjct: 218 DTVISVMDAAGTPDAANYFPFLRFLDLQGNVKTFKVCTERLVRVFRGFIDAKIAEKSSQN 277

Query: 277 S----ASSDFLDVLI 281
           +    + +DF+D L+
Sbjct: 278 NPKDVSKNDFVDNLL 287

[Close]

BLAST of HG10009458 vs. TAIR 10
Match: AT2G45560.2 (cytochrome P450, family 76, subfamily C, polypeptide 1 )

HSP 1 Score: 139.4 bits (350), Expect = 4.6e-33
Identity = 87/255 (34.12%), Postives = 141/255 (55.29%), Query Frame = 0


Query: 37  LPPGPRPWPILGNLLQIGQNAHISMTQFAKIHGPLISLKLGAQCLVVASSPAAATAILKT 96
           LPPGP   PI+GN+  +G++ H S  + +K +GP++SLKLG+   VV +SP AA  +L+T
Sbjct: 38  LPPGPPRLPIIGNIHLVGKHPHRSFAELSKTYGPVMSLKLGSLNTVVIASPEAAREVLRT 97

Query: 97  QDRLLSARYIFQMTPDRAL----HDRCSLVFSPECGDLWKNLRSICKANLFTAKAIESQA 156
            D++LSAR     +P  A+    H   SLV+ P     W+ LR +    L + + IE+  
Sbjct: 98  HDQILSAR-----SPTNAVRSINHQDASLVWLPSSSARWRLLRRLSVTQLLSPQRIEATK 157

Query: 157 CLREKKMKDLVEFL--ESKQGSVVEIKDFVFTSVFNTLSNLIFSMDLLGF-VGDGFNGIK 216
            LR  K+K+LV F+   S +   V+I    F +  N +SN++FS+DL  +      NG++
Sbjct: 158 ALRMNKVKELVSFISESSDREESVDISRVAFITTLNIISNILFSVDLGSYNAKASINGVQ 217

Query: 217 GPFKKMMDLGLTPNLADFYPLLRSFDLQGLRKKAAIYKNQVDSLWGGLIEERREIHSQQG 276
                +MD   TP+ A+++P LR  DLQG  K   +   ++  ++ G I+ +    S Q 
Sbjct: 218 DTVISVMDAAGTPDAANYFPFLRFLDLQGNVKTFKVCTERLVRVFRGFIDAKIAEKSSQN 277

Query: 277 S----ASSDFLDVLI 281
           +    + +DF+D L+
Sbjct: 278 NPKDVSKNDFVDNLL 287

[Close]

BLAST of HG10009458 vs. TAIR 10
Match: AT3G52970.2 (cytochrome P450, family 76, subfamily G, polypeptide 1 )

HSP 1 Score: 137.5 bits (345), Expect = 1.7e-32
Identity = 85/290 (29.31%), Postives = 147/290 (50.69%), Query Frame = 0


Query: 37  LPPGPRPWPILGNLLQI-GQNAHISMTQFAKIHGPLISLKLGAQCLVVASSPAAATAILK 96
           LPPGP PWP++GN+ Q+ G   H S+T+ ++ HGP+++L++G+   VV SS   A  I K
Sbjct: 36  LPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHGPIMTLRIGSMLTVVISSSEVAREIFK 95

Query: 97  TQDRLLSARYIFQMTPDRALHDRCSLVFSPECGDLWKNLRSICKANLFTAKAIESQACLR 156
             D  L+ R I++        D    + + + G  W+ LR +C    F  + +++ + +R
Sbjct: 96  KHDAALAGRKIYEAMKGGKSSD--GSLITAQYGAYWRMLRRLCTTQFFVTRRLDAMSDVR 155

Query: 157 EKKMKDLVEFLE--SKQG---------------SVVEIKDFVFTSVFNTLSNLIFSMDLL 216
            + +  ++ F+E   + G                 +++  + F   FN + NL+FS DLL
Sbjct: 156 SRCVDQMLRFVEEGGQNGMYYTINNIMTCIICTKTIDVGRYFFLMAFNLIGNLMFSRDLL 215

Query: 217 GFVGDGFNGIKGPFK--KMMDLGLTPNLADFYPLLRSFDLQGLRKKAAIYKNQVDSLWGG 276
               D   G +  +   K+M+    PN+ADF+PLLR  D QG+R+K   +  +   + G 
Sbjct: 216 D--PDSKRGSEFFYHTGKVMEFAGKPNVADFFPLLRFLDPQGIRRKTQFHVEKAFEIAGE 275

Query: 277 LIEERREIHSQQGS--ASSDFLDVLIQ-----------SGFTDQQINALI 294
            I ER E+  ++ S   + D+LDVL++           S F+ + IN ++
Sbjct: 276 FIRERTEVREREKSDEKTKDYLDVLLEFRGGDGVDEEPSSFSSRDINVIV 321

The following BLAST results are available for this feature:

BLAST of HG10009458 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038906737.1	1.5e-142	90.14	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Benincasa hispida]	[more]
TYK00293.1	2.4e-137	88.47	putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo var. maku...	[more]
XP_008467223.1	5.4e-137	88.44	PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis mel...	[more]
KAA0055170.1	9.3e-137	88.10	putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo var. maku...	[more]
KAE8647950.1	5.1e-135	87.37	hypothetical protein Csa_000545 [Cucumis sativus]	[more]

BLAST of HG10009458 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O64900	2.2e-56	42.20	(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3...	[more]
Q9SP06	2.9e-56	44.66	(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum...	[more]
O64899	1.1e-55	41.61	(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia calif...	[more]
Q9FXW4	1.0e-53	43.77	Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3...	[more]
I3V6B1	8.6e-53	41.84	(S)-N-methylcoclaurine 3'-hydroxylase-like protein OS=Papaver somniferum OX=3469...	[more]

BLAST of HG10009458 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BKL7	1.2e-137	88.47	Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. ma...	[more]
A0A1S3CT14	2.6e-137	88.44	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656...	[more]
A0A5A7UIU3	4.5e-137	88.10	Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. ma...	[more]
A0A0A0KRT5	3.2e-135	87.12	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G173480 PE=4 SV=1	[more]
A0A0A0KPU9	1.0e-133	84.75	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G173490 PE=4 SV=1	[more]

BLAST of HG10009458 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G52970.1	3.2e-34	30.80	cytochrome P450, family 76, subfamily G, polypeptide 1	[more]
AT2G45570.1	2.1e-33	32.76	cytochrome P450, family 76, subfamily C, polypeptide 2	[more]
AT2G45560.1	4.6e-33	34.12	cytochrome P450, family 76, subfamily C, polypeptide 1	[more]
AT2G45560.2	4.6e-33	34.12	cytochrome P450, family 76, subfamily C, polypeptide 1	[more]
AT3G52970.2	1.7e-32	29.31	cytochrome P450, family 76, subfamily G, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 22..296 e-value: 3.7E-39 score: 136.6
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 33..294
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 38..293 e-value: 7.7E-28 score: 97.4
None	No IPR available	PANTHER	PTHR47956	CYTOCHROME P450 71B11-RELATED	coord: 11..293
None	No IPR available	PANTHER	PTHR47956:SF23	(S)-N-METHYLCOCLAURINE 3'-HYDROXYLASE ISOZYME 1-LIKE	coord: 11..293

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10009458.1	HG10009458.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen