HG10009362 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10009362
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	4a-hydroxytetrahydrobiopterin dehydratase
Location	Chr06: 5099326 .. 5100978 (-)
RNA-Seq Expression	HG10009362
Synteny	HG10009362

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCACAGCCTCATCCACCCACCTCCACCTCCCTCTCCTCCCTCTTCCTCATCCCCACCGCCGCTTCTCTCCCCCTCCCACCACCCTCCTCCGCCGCAACCACCGCTCAGCCTCCATCATAGTCTCCGTAGGAGCCGACAAACTGGGGGACTTCGGCGCGAGAGACCCATTTCCGGCGGAAATCGAGAGCAATTTCGGGGAAAAAGTGGTGGGATTTTCCGATACAGAGCACAAGATTCTGATCCCTAATGTCCGAGCCTTGGCTCTGTCTCAGCAACAGTGTGTTCCAGTTTCTTCACTGCAAACTCCAATGGCAGAGGATGAAGCCCAAAAGCTCTTGAAGAAGGTATTATTCTTTGATTTTGATTTTGATTTTGATTTTATTGTAATTTTTGTGGGTTTTCTTTTAATTAGGTGGTGGGTTGGAGATTGGTGGATGATGGAACAGGGCAATTTAAGCTTCAATGTTTGTGGAAATTGAGGGATTTTAAATGCAGTGTTGAATTGATTAATAGAATCTTTAAGGTTGTTGAAAATGTTGGGCATTTCCCTGATCTTCATTTGGAGCAGCCTAATCAAGTTAGAGCTGAGCTTTGGACTTCACCCATTGGTAATACTCTCTTCTCTCTCTCAAAACACTTCCCCCATCTAAATTGACACCCCAAAATTAATTAACCTATGTTAATTTTTTTAATTAAAAAAATTAAATGATTATCAAATGAGACATTAACATTATTGAAATTCGGTGTTAGTTTTATCTCTGTAATAGAATTCTTGATTGAATTTTATGATATAATATCATAGTGCTGAATCTAAAAAGATTTTCGTAGATTATTTTACATTTGGTTCTTAATTTTTTAAAATTTTCAATTTTATTTGTAAGGTGTTAGAATTTGTTAGTTAGATAATTCCATTAAAAAAACTGTCATAAAAAAGTTAACATAGATTAATGTTTTTTAGTAGAATGGCTAGATTATTGATTTATATACATGCTAATTATTTTATGTAGGTATGCTCTTTCTTTTATAGTTAGATGTTCAAGTGACATTTCCACACGTCGGGATATAATATTAAACATTAAATTGAAAGTTACCTAAAAAGATTAGACTTTTGTACAAATGACTCAAAATTTACTGCGGAAATGTCGTTTGGCCCAAATTTTGAAAATAAAGATGTACAACCTTTTACTTACGTCATTCAACTAATCTAATATAAAACTCTTCCTCGTCCACTCTCTTGCGCTCATGGTCCTTCTTGCTCGTGCTTGTGCTCACTTCTCTGAAAAGAAAAGAAAAAACGTTATTTGATTTCTGACATTCGTAAAATATTTTTCTTGATTGTTTTTAAAATTTGGGTTAAACGACATTTTGGGTGTGAAACTTGAGCCATCAATTTAAAATTTTTCAATTGGAATAAGATTGAACCTTCAACTTCAATTGGAATTCTATACTAATTTTTTCAAGTGTGGTATAGTAAGAAAATATTAAAAATTATGATAAAAGTTAAATATACCAAAATTTATAAAATGGAGACGTGTAATTATGGTTTTGAATTTGCAGGAGGTTTGAGTATGAATGATTTTATTGTAGCTGCAAAAATTGATGAGATAAAAACATCAGATCTTGCTCCCAGGAAAAGAGTTTGGGCATAG

mRNA sequence

ATGGCCACAGCCTCATCCACCCACCTCCACCTCCCTCTCCTCCCTCTTCCTCATCCCCACCGCCGCTTCTCTCCCCCTCCCACCACCCTCCTCCGCCGCAACCACCGCTCAGCCTCCATCATAGTCTCCGTAGGAGCCGACAAACTGGGGGACTTCGGCGCGAGAGACCCATTTCCGGCGGAAATCGAGAGCAATTTCGGGGAAAAAGTGGTGGGATTTTCCGATACAGAGCACAAGATTCTGATCCCTAATGTCCGAGCCTTGGCTCTGTCTCAGCAACAGTGTGTTCCAGTTTCTTCACTGCAAACTCCAATGGCAGAGGATGAAGCCCAAAAGCTCTTGAAGAAGGTGGTGGGTTGGAGATTGGTGGATGATGGAACAGGGCAATTTAAGCTTCAATGTTTGTGGAAATTGAGGGATTTTAAATGCAGTGTTGAATTGATTAATAGAATCTTTAAGGTTGTTGAAAATGTTGGGCATTTCCCTGATCTTCATTTGGAGCAGCCTAATCAAGTTAGAGCTGAGCTTTGGACTTCACCCATTGGAGGTTTGAGTATGAATGATTTTATTGTAGCTGCAAAAATTGATGAGATAAAAACATCAGATCTTGCTCCCAGGAAAAGAGTTTGGGCATAG

Coding sequence (CDS)

ATGGCCACAGCCTCATCCACCCACCTCCACCTCCCTCTCCTCCCTCTTCCTCATCCCCACCGCCGCTTCTCTCCCCCTCCCACCACCCTCCTCCGCCGCAACCACCGCTCAGCCTCCATCATAGTCTCCGTAGGAGCCGACAAACTGGGGGACTTCGGCGCGAGAGACCCATTTCCGGCGGAAATCGAGAGCAATTTCGGGGAAAAAGTGGTGGGATTTTCCGATACAGAGCACAAGATTCTGATCCCTAATGTCCGAGCCTTGGCTCTGTCTCAGCAACAGTGTGTTCCAGTTTCTTCACTGCAAACTCCAATGGCAGAGGATGAAGCCCAAAAGCTCTTGAAGAAGGTGGTGGGTTGGAGATTGGTGGATGATGGAACAGGGCAATTTAAGCTTCAATGTTTGTGGAAATTGAGGGATTTTAAATGCAGTGTTGAATTGATTAATAGAATCTTTAAGGTTGTTGAAAATGTTGGGCATTTCCCTGATCTTCATTTGGAGCAGCCTAATCAAGTTAGAGCTGAGCTTTGGACTTCACCCATTGGAGGTTTGAGTATGAATGATTTTATTGTAGCTGCAAAAATTGATGAGATAAAAACATCAGATCTTGCTCCCAGGAAAAGAGTTTGGGCATAG

Protein sequence

MATASSTHLHLPLLPLPHPHRRFSPPPTTLLRRNHRSASIIVSVGADKLGDFGARDPFPAEIESNFGEKVVGFSDTEHKILIPNVRALALSQQQCVPVSSLQTPMAEDEAQKLLKKVVGWRLVDDGTGQFKLQCLWKLRDFKCSVELINRIFKVVENVGHFPDLHLEQPNQVRAELWTSPIGGLSMNDFIVAAKIDEIKTSDLAPRKRVWA

Homology

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BLAST of HG10009362 vs. NCBI nr
Match: XP_038877111.1 (probable pterin-4-alpha-carbinolamine dehydratase, chloroplastic [Benincasa hispida])

HSP 1 Score: 404.1 bits (1037), Expect = 7.6e-109
Identity = 198/211 (93.84%), Postives = 201/211 (95.26%), Query Frame = 0


Query: 1   MATASSTHLHLPLLPLPHPHRRFSPPPTTLLRRNHRSASIIVSVGADKLGDFGARDPFPA 60
           MATASSTHLHLPLLPLPH  RRFS PP  LLRRN RSAS+IVSVGADKLGDFGARDPFPA
Sbjct: 1   MATASSTHLHLPLLPLPHHRRRFSSPPAILLRRNRRSASVIVSVGADKLGDFGARDPFPA 60

Query: 61  EIESNFGEKVVGFSDTEHKILIPNVRALALSQQQCVPVSSLQTPMAEDEAQKLLKKVVGW 120
           EIESNFGEKVVGFSDTEHKILIPNVRALALSQQ+CVPVSSLQ PMAEDEAQKLLKKVVGW
Sbjct: 61  EIESNFGEKVVGFSDTEHKILIPNVRALALSQQECVPVSSLQAPMAEDEAQKLLKKVVGW 120

Query: 121 RLVDDGTGQFKLQCLWKLRDFKCSVELINRIFKVVENVGHFPDLHLEQPNQVRAELWTSP 180
           RLVDDG G+FKLQCLWKLRDFKC VELINRI KVVENVGHFPDLHLEQPNQVRAELWTSP
Sbjct: 121 RLVDDGRGEFKLQCLWKLRDFKCCVELINRISKVVENVGHFPDLHLEQPNQVRAELWTSP 180

Query: 181 IGGLSMNDFIVAAKIDEIKTSDLAPRKRVWA 212
           IGGLSMNDFIVAAKIDEIKTSDLAPRKRVWA
Sbjct: 181 IGGLSMNDFIVAAKIDEIKTSDLAPRKRVWA 211

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BLAST of HG10009362 vs. NCBI nr
Match: XP_008436971.1 (PREDICTED: putative pterin-4-alpha-carbinolamine dehydratase [Cucumis melo] >KAA0055083.1 putative pterin-4-alpha-carbinolamine dehydratase [Cucumis melo var. makuwa])

HSP 1 Score: 360.5 bits (924), Expect = 9.7e-96
Identity = 181/214 (84.58%), Postives = 193/214 (90.19%), Query Frame = 0


Query: 1   MATASST--HLHLPLLPLPHPHR-RFSPPPTTLLRRNHRSASIIVSVGADKLGDFGARDP 60
           MAT SST  HLHLPLLPLP PHR RFS  PTTLLRR+ R  S+ VSVG D LGDFGARDP
Sbjct: 1   MATTSSTHLHLHLPLLPLPQPHRPRFSSLPTTLLRRSRR--SVTVSVGTDILGDFGARDP 60

Query: 61  FPAEIESNFGEKVVGFSDTEHKILIPNVRALALSQQQCVPVSSLQTPMAEDEAQKLLKKV 120
           FPAEIESNFG+KV+G+S+TEHKILIPN RALALSQQ+CVPVSSLQ PMAEDEA+ LLKKV
Sbjct: 61  FPAEIESNFGDKVIGYSNTEHKILIPNARALALSQQECVPVSSLQAPMAEDEAKMLLKKV 120

Query: 121 VGWRLVDDGTGQFKLQCLWKLRDFKCSVELINRIFKVVENVGHFPDLHLEQPNQVRAELW 180
           VGW+LVDDG+G  KLQCLWKLRDFKCSVELINRI +VVENVGHFPDLHLEQPNQVRAELW
Sbjct: 121 VGWKLVDDGSGGLKLQCLWKLRDFKCSVELINRICQVVENVGHFPDLHLEQPNQVRAELW 180

Query: 181 TSPIGGLSMNDFIVAAKIDEIKTSDLAPRKRVWA 212
           TS IGGLSMND+IVAAKIDEIKTSDLAPRKR WA
Sbjct: 181 TSSIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 212

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BLAST of HG10009362 vs. NCBI nr
Match: XP_004143676.1 (probable pterin-4-alpha-carbinolamine dehydratase, chloroplastic [Cucumis sativus] >KGN50323.1 hypothetical protein Csa_000223 [Cucumis sativus])

HSP 1 Score: 359.4 bits (921), Expect = 2.2e-95
Identity = 181/214 (84.58%), Postives = 190/214 (88.79%), Query Frame = 0


Query: 1   MATASST--HLHLPLLPLPHPHRR-FSPPPTTLLRRNHRSASIIVSVGADKLGDFGARDP 60
           MAT SST  HLHLPLLPLP PH R F P PTTLLRRN R  S+ VSVG D LGDFGARDP
Sbjct: 1   MATTSSTHLHLHLPLLPLPQPHHRTFLPLPTTLLRRNLR--SLTVSVGTDILGDFGARDP 60

Query: 61  FPAEIESNFGEKVVGFSDTEHKILIPNVRALALSQQQCVPVSSLQTPMAEDEAQKLLKKV 120
           FP EI SNFGEKV+GFSDTEHKILIPN RALALS+Q+CVPVSSLQ PMAEDEA+KLL+KV
Sbjct: 61  FPEEIASNFGEKVIGFSDTEHKILIPNARALALSEQECVPVSSLQAPMAEDEAKKLLRKV 120

Query: 121 VGWRLVDDGTGQFKLQCLWKLRDFKCSVELINRIFKVVENVGHFPDLHLEQPNQVRAELW 180
           VGW+LVD G G+ KLQCLWKLRDFKCSVELINRI KVVENVGHFPDLHLEQPNQVRAELW
Sbjct: 121 VGWKLVDGGRGELKLQCLWKLRDFKCSVELINRICKVVENVGHFPDLHLEQPNQVRAELW 180

Query: 181 TSPIGGLSMNDFIVAAKIDEIKTSDLAPRKRVWA 212
           TS IGGLSMND+IVAAKIDEIKTSDLAPRKR WA
Sbjct: 181 TSSIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 212

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BLAST of HG10009362 vs. NCBI nr
Match: XP_022156424.1 (uncharacterized protein LOC111023314 [Momordica charantia])

HSP 1 Score: 323.9 bits (829), Expect = 1.0e-84
Identity = 165/214 (77.10%), Postives = 183/214 (85.51%), Query Frame = 0


Query: 1   MATASSTHLHLPLLPLPHPHRRFSPPP--TTLLRRNHRSASIIVSVGADKLGDFGARDPF 60
           MAT SSTHL LPLL L  PHRRFSPP   TT+LR   R A++  ++  D  GDFGARDPF
Sbjct: 12  MATTSSTHLPLPLLHL--PHRRFSPPTPRTTVLRHWRRPATVRAAIQGD--GDFGARDPF 71

Query: 61  PAEIESNFGEKVVGFSDTEHKILIPNVRALALSQQQCVPVSSLQTPMAEDEAQKLLKKVV 120
           PAEIES FGEKV+GFSDTEHKILIP++ ALALSQQQC PVSSL  PMA+DEAQKLLKKVV
Sbjct: 72  PAEIESRFGEKVLGFSDTEHKILIPSIPALALSQQQCAPVSSLGPPMADDEAQKLLKKVV 131

Query: 121 GWRLVD-DGTGQFKLQCLWKLRDFKCSVELINRIFKVVENVGHFPDLHLEQPNQVRAELW 180
           GWRLVD DG+G+ KLQCLWKLRDF+C VELI RI++VV++ GHFPDLHLE  NQVRAELW
Sbjct: 132 GWRLVDNDGSGRLKLQCLWKLRDFECGVELIKRIYEVVQDCGHFPDLHLEHSNQVRAELW 191

Query: 181 TSPIGGLSMNDFIVAAKIDEIKTSDLAPRKRVWA 212
           TS IGGLSMNDFIVAAK+DEI+TSDLAPRKRVWA
Sbjct: 192 TSAIGGLSMNDFIVAAKLDEIRTSDLAPRKRVWA 221

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BLAST of HG10009362 vs. NCBI nr
Match: XP_022996033.1 (uncharacterized protein LOC111491359 [Cucurbita maxima] >XP_023532646.1 uncharacterized protein LOC111794746 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 322.8 bits (826), Expect = 2.2e-84
Identity = 162/211 (76.78%), Postives = 177/211 (83.89%), Query Frame = 0


Query: 4   ASSTHLHLPLLPLPHPHRRFSPPP---TTLLRRNHRSASIIVSVGADKLGDFGARDPFPA 63
           A++   + PLL LP PH RFS PP   TTLLRR  R A+I  +V  D  GDFGARDPFPA
Sbjct: 2   ATTPTANFPLLRLPRPHSRFSLPPSPSTTLLRRKLRPATIRAAVVGD--GDFGARDPFPA 61

Query: 64  EIESNFGEKVVGFSDTEHKILIPNVRALALSQQQCVPVSSLQTPMAEDEAQKLLKKVVGW 123
           EIESNFG+KV+GFS+TEHKILIPNVRALALSQQQCVP+SSLQ PM EDEAQKLLKKVVGW
Sbjct: 62  EIESNFGDKVIGFSNTEHKILIPNVRALALSQQQCVPISSLQAPMPEDEAQKLLKKVVGW 121

Query: 124 RLVDDGTGQFKLQCLWKLRDFKCSVELINRIFKVVENVGHFPDLHLEQPNQVRAELWTSP 183
           RLVD+G GQFKL+CLWKLRDFKC VELINRI   V++  HFPD+HL+QPNQV AELWTS 
Sbjct: 122 RLVDNGRGQFKLRCLWKLRDFKCCVELINRISNAVDDANHFPDIHLDQPNQVTAELWTSA 181

Query: 184 IGGLSMNDFIVAAKIDEIKTSDLAPRKRVWA 212
           IGGLSMNDFIVAAKIDEIKT DLA RKR WA
Sbjct: 182 IGGLSMNDFIVAAKIDEIKTIDLAARKRAWA 210

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BLAST of HG10009362 vs. ExPASy Swiss-Prot
Match: Q9LU63 (Probable pterin-4-alpha-carbinolamine dehydratase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATP1 PE=1 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 8.6e-63
Identity = 116/187 (62.03%), Postives = 140/187 (74.87%), Query Frame = 0


Query: 25  PPPTTLLRRNHRSASIIVSVGADKLGDFGARDPFPAEIESNFGEKVVGFSDTEHKILIPN 84
           PP +   R+  R      ++  D LGDFGARDP+P EI S FG+KV+G   TEHKILIPN
Sbjct: 34  PPVSRNNRKLGRLTVTRSNLAQDFLGDFGARDPYPEEIASQFGDKVLGCQSTEHKILIPN 93

Query: 85  VRALALSQQQCVPVSSLQTPMAEDEAQKLLKKVVGWRLVDDGTGQFKLQCLWKLRDFKCS 144
              L+LSQ QC PVSS Q P++ D+A+ LL KV+GW +VD+  G  K++C+WK+RDF C 
Sbjct: 94  ASVLSLSQLQCSPVSSSQPPLSGDDARTLLHKVLGWSIVDNEAGGLKIRCMWKVRDFGCG 153

Query: 145 VELINRIFKVVENVGHFPDLHLEQPNQVRAELWTSPIGGLSMNDFIVAAKIDEIKTSDLA 204
           VELINRI KV E  GH+P LHLE P QVRAEL+TS IGGLSMNDFI+AAKID+IKTSDL+
Sbjct: 154 VELINRIHKVAEASGHYPSLHLESPTQVRAELFTSSIGGLSMNDFIMAAKIDDIKTSDLS 213

Query: 205 PRKRVWA 212
           PRKR WA
Sbjct: 214 PRKRAWA 220

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BLAST of HG10009362 vs. ExPASy Swiss-Prot
Match: Q6QJ72 (Pterin-4-alpha-carbinolamine dehydratase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PDL2 PE=1 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 7.3e-22
Identity = 52/121 (42.98%), Postives = 75/121 (61.98%), Query Frame = 0


Query: 90  LSQQQCVPVSSLQ-TPMAEDEAQKLLKKVVGWRLVDDGTGQFKLQCLWKLRDFKCSVELI 149
           LS ++CVP ++     M E  AQ LL+KV GW L +D     KL   W+++ F   ++  
Sbjct: 66  LSTKKCVPCNAKDLRAMTEQSAQDLLQKVAGWDLAND-NDTLKLHRSWRVKSFTKGLDFF 125

Query: 150 NRIFKVVENVGHFPDLHLEQPNQVRAELWTSPIGGLSMNDFIVAAKIDEIKTSDLAPRKR 209
            R+  + E+ GH PDLHL   N V+ E+WT  IGGL+ NDFI+AAKI+E++  DL  +K+
Sbjct: 126 QRVADIAESEGHHPDLHLVGWNNVKIEIWTHAIGGLTENDFILAAKINELQVEDLLRKKK 185

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BLAST of HG10009362 vs. ExPASy Swiss-Prot
Match: B0JQV0 (Putative pterin-4-alpha-carbinolamine dehydratase OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=MAE_39970 PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 3.1e-12
Identity = 39/109 (35.78%), Postives = 61/109 (55.96%), Query Frame = 0


Query: 90  LSQQQCVPVSSLQTPMAEDEAQKLLKKVVGWRLVDDGTGQFKLQCLWKLRDFKCSVELIN 149
           L+QQ+C P  S  +P+  +E   L  K+  W L+ +  G  +LQ L+K  +F+ ++   N
Sbjct: 4   LAQQKCQPYQSGSSPITAEEITALQAKIPDWNLL-EYEGIPRLQKLYKFANFQGAIAFTN 63

Query: 150 RIFKVVENVGHFPDLHLEQPNQVRAELWTSPIGGLSMNDFIVAAKIDEI 199
            + +  E  GH P L L +  +V    WT  +GGL  NDFI+AA+ D+I
Sbjct: 64  AVGEAAEKEGHHPAL-LTEWGKVTVSWWTHDVGGLHQNDFIMAARTDDI 110

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BLAST of HG10009362 vs. ExPASy Swiss-Prot
Match: Q60BH8 (Putative pterin-4-alpha-carbinolamine dehydratase OS=Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) OX=243233 GN=MCA0497 PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 4.5e-11
Identity = 39/112 (34.82%), Postives = 61/112 (54.46%), Query Frame = 0


Query: 87  ALALSQQQCVPVSSLQTPMAEDEAQKLLKKVVGWRLVDDGTGQFKLQCLWKLRDFKCSVE 146
           A +L+ +QC P      P+  +EA+KLL  V  W L D  T   KL+  ++  +F  +++
Sbjct: 3   ACSLTAKQCTPCQGGIPPLTAEEAEKLLVHVPRWELKDAAT---KLKRTFRFENFMEALD 62

Query: 147 LINRIFKVVENVGHFPDLHLEQPNQVRAELWTSPIGGLSMNDFIVAAKIDEI 199
              ++ ++ E  GH PD+ +      R E  T  I GL  NDFI+AAK+DE+
Sbjct: 63  FARKVGELCEAEGHHPDIGIGW-GYCRVEFQTHKINGLHENDFIMAAKVDEL 110

[Close]

BLAST of HG10009362 vs. ExPASy Swiss-Prot
Match: Q3B6N5 (Putative pterin-4-alpha-carbinolamine dehydratase OS=Pelodictyon luteolum (strain DSM 273 / 2530) OX=319225 GN=Plut_0106 PE=3 SV=2)

HSP 1 Score: 67.0 bits (162), Expect = 2.9e-10
Identity = 36/109 (33.03%), Postives = 63/109 (57.80%), Query Frame = 0


Query: 90  LSQQQCVPVSSLQTPMAEDEAQKLLKKVVGWRLVDDGTGQFKLQCLWKLRDFKCSVELIN 149
           L++ +CV  S    P+AE E+++LLK++  W +V +  G  +L   +   +F+ ++    
Sbjct: 4   LNKTKCVSCSEGLPPLAERESEELLKEIPEWVIVSE-DGVSRLVRTFTFENFREAMAFAG 63

Query: 150 RIFKVVENVGHFPDLHLEQPNQVRAELWTSPIGGLSMNDFIVAAKIDEI 199
            + ++ E+  H P L  E   +VR E WT  + GL MNDF++AA+ DE+
Sbjct: 64  SVGELAESEQHHPKLVTEW-GKVRVEWWTHAVHGLHMNDFVMAARTDEL 110

[Close]

BLAST of HG10009362 vs. ExPASy TrEMBL
Match: A0A5A7UGL0 (4a-hydroxytetrahydrobiopterin dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00090 PE=3 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 4.7e-96
Identity = 181/214 (84.58%), Postives = 193/214 (90.19%), Query Frame = 0


Query: 1   MATASST--HLHLPLLPLPHPHR-RFSPPPTTLLRRNHRSASIIVSVGADKLGDFGARDP 60
           MAT SST  HLHLPLLPLP PHR RFS  PTTLLRR+ R  S+ VSVG D LGDFGARDP
Sbjct: 1   MATTSSTHLHLHLPLLPLPQPHRPRFSSLPTTLLRRSRR--SVTVSVGTDILGDFGARDP 60

Query: 61  FPAEIESNFGEKVVGFSDTEHKILIPNVRALALSQQQCVPVSSLQTPMAEDEAQKLLKKV 120
           FPAEIESNFG+KV+G+S+TEHKILIPN RALALSQQ+CVPVSSLQ PMAEDEA+ LLKKV
Sbjct: 61  FPAEIESNFGDKVIGYSNTEHKILIPNARALALSQQECVPVSSLQAPMAEDEAKMLLKKV 120

Query: 121 VGWRLVDDGTGQFKLQCLWKLRDFKCSVELINRIFKVVENVGHFPDLHLEQPNQVRAELW 180
           VGW+LVDDG+G  KLQCLWKLRDFKCSVELINRI +VVENVGHFPDLHLEQPNQVRAELW
Sbjct: 121 VGWKLVDDGSGGLKLQCLWKLRDFKCSVELINRICQVVENVGHFPDLHLEQPNQVRAELW 180

Query: 181 TSPIGGLSMNDFIVAAKIDEIKTSDLAPRKRVWA 212
           TS IGGLSMND+IVAAKIDEIKTSDLAPRKR WA
Sbjct: 181 TSSIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 212

[Close]

BLAST of HG10009362 vs. ExPASy TrEMBL
Match: A0A1S3ASJ9 (4a-hydroxytetrahydrobiopterin dehydratase OS=Cucumis melo OX=3656 GN=LOC103482540 PE=3 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 4.7e-96
Identity = 181/214 (84.58%), Postives = 193/214 (90.19%), Query Frame = 0


Query: 1   MATASST--HLHLPLLPLPHPHR-RFSPPPTTLLRRNHRSASIIVSVGADKLGDFGARDP 60
           MAT SST  HLHLPLLPLP PHR RFS  PTTLLRR+ R  S+ VSVG D LGDFGARDP
Sbjct: 1   MATTSSTHLHLHLPLLPLPQPHRPRFSSLPTTLLRRSRR--SVTVSVGTDILGDFGARDP 60

Query: 61  FPAEIESNFGEKVVGFSDTEHKILIPNVRALALSQQQCVPVSSLQTPMAEDEAQKLLKKV 120
           FPAEIESNFG+KV+G+S+TEHKILIPN RALALSQQ+CVPVSSLQ PMAEDEA+ LLKKV
Sbjct: 61  FPAEIESNFGDKVIGYSNTEHKILIPNARALALSQQECVPVSSLQAPMAEDEAKMLLKKV 120

Query: 121 VGWRLVDDGTGQFKLQCLWKLRDFKCSVELINRIFKVVENVGHFPDLHLEQPNQVRAELW 180
           VGW+LVDDG+G  KLQCLWKLRDFKCSVELINRI +VVENVGHFPDLHLEQPNQVRAELW
Sbjct: 121 VGWKLVDDGSGGLKLQCLWKLRDFKCSVELINRICQVVENVGHFPDLHLEQPNQVRAELW 180

Query: 181 TSPIGGLSMNDFIVAAKIDEIKTSDLAPRKRVWA 212
           TS IGGLSMND+IVAAKIDEIKTSDLAPRKR WA
Sbjct: 181 TSSIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 212

[Close]

BLAST of HG10009362 vs. ExPASy TrEMBL
Match: A0A0A0KL10 (4a-hydroxytetrahydrobiopterin dehydratase OS=Cucumis sativus OX=3659 GN=Csa_5G167150 PE=3 SV=1)

HSP 1 Score: 359.4 bits (921), Expect = 1.0e-95
Identity = 181/214 (84.58%), Postives = 190/214 (88.79%), Query Frame = 0


Query: 1   MATASST--HLHLPLLPLPHPHRR-FSPPPTTLLRRNHRSASIIVSVGADKLGDFGARDP 60
           MAT SST  HLHLPLLPLP PH R F P PTTLLRRN R  S+ VSVG D LGDFGARDP
Sbjct: 1   MATTSSTHLHLHLPLLPLPQPHHRTFLPLPTTLLRRNLR--SLTVSVGTDILGDFGARDP 60

Query: 61  FPAEIESNFGEKVVGFSDTEHKILIPNVRALALSQQQCVPVSSLQTPMAEDEAQKLLKKV 120
           FP EI SNFGEKV+GFSDTEHKILIPN RALALS+Q+CVPVSSLQ PMAEDEA+KLL+KV
Sbjct: 61  FPEEIASNFGEKVIGFSDTEHKILIPNARALALSEQECVPVSSLQAPMAEDEAKKLLRKV 120

Query: 121 VGWRLVDDGTGQFKLQCLWKLRDFKCSVELINRIFKVVENVGHFPDLHLEQPNQVRAELW 180
           VGW+LVD G G+ KLQCLWKLRDFKCSVELINRI KVVENVGHFPDLHLEQPNQVRAELW
Sbjct: 121 VGWKLVDGGRGELKLQCLWKLRDFKCSVELINRICKVVENVGHFPDLHLEQPNQVRAELW 180

Query: 181 TSPIGGLSMNDFIVAAKIDEIKTSDLAPRKRVWA 212
           TS IGGLSMND+IVAAKIDEIKTSDLAPRKR WA
Sbjct: 181 TSSIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 212

[Close]

BLAST of HG10009362 vs. ExPASy TrEMBL
Match: A0A6J1DTF1 (4a-hydroxytetrahydrobiopterin dehydratase OS=Momordica charantia OX=3673 GN=LOC111023314 PE=3 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 4.8e-85
Identity = 165/214 (77.10%), Postives = 183/214 (85.51%), Query Frame = 0


Query: 1   MATASSTHLHLPLLPLPHPHRRFSPPP--TTLLRRNHRSASIIVSVGADKLGDFGARDPF 60
           MAT SSTHL LPLL L  PHRRFSPP   TT+LR   R A++  ++  D  GDFGARDPF
Sbjct: 12  MATTSSTHLPLPLLHL--PHRRFSPPTPRTTVLRHWRRPATVRAAIQGD--GDFGARDPF 71

Query: 61  PAEIESNFGEKVVGFSDTEHKILIPNVRALALSQQQCVPVSSLQTPMAEDEAQKLLKKVV 120
           PAEIES FGEKV+GFSDTEHKILIP++ ALALSQQQC PVSSL  PMA+DEAQKLLKKVV
Sbjct: 72  PAEIESRFGEKVLGFSDTEHKILIPSIPALALSQQQCAPVSSLGPPMADDEAQKLLKKVV 131

Query: 121 GWRLVD-DGTGQFKLQCLWKLRDFKCSVELINRIFKVVENVGHFPDLHLEQPNQVRAELW 180
           GWRLVD DG+G+ KLQCLWKLRDF+C VELI RI++VV++ GHFPDLHLE  NQVRAELW
Sbjct: 132 GWRLVDNDGSGRLKLQCLWKLRDFECGVELIKRIYEVVQDCGHFPDLHLEHSNQVRAELW 191

Query: 181 TSPIGGLSMNDFIVAAKIDEIKTSDLAPRKRVWA 212
           TS IGGLSMNDFIVAAK+DEI+TSDLAPRKRVWA
Sbjct: 192 TSAIGGLSMNDFIVAAKLDEIRTSDLAPRKRVWA 221

[Close]

BLAST of HG10009362 vs. ExPASy TrEMBL
Match: A0A6J1K9P8 (4a-hydroxytetrahydrobiopterin dehydratase OS=Cucurbita maxima OX=3661 GN=LOC111491359 PE=3 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 1.1e-84
Identity = 162/211 (76.78%), Postives = 177/211 (83.89%), Query Frame = 0


Query: 4   ASSTHLHLPLLPLPHPHRRFSPPP---TTLLRRNHRSASIIVSVGADKLGDFGARDPFPA 63
           A++   + PLL LP PH RFS PP   TTLLRR  R A+I  +V  D  GDFGARDPFPA
Sbjct: 2   ATTPTANFPLLRLPRPHSRFSLPPSPSTTLLRRKLRPATIRAAVVGD--GDFGARDPFPA 61

Query: 64  EIESNFGEKVVGFSDTEHKILIPNVRALALSQQQCVPVSSLQTPMAEDEAQKLLKKVVGW 123
           EIESNFG+KV+GFS+TEHKILIPNVRALALSQQQCVP+SSLQ PM EDEAQKLLKKVVGW
Sbjct: 62  EIESNFGDKVIGFSNTEHKILIPNVRALALSQQQCVPISSLQAPMPEDEAQKLLKKVVGW 121

Query: 124 RLVDDGTGQFKLQCLWKLRDFKCSVELINRIFKVVENVGHFPDLHLEQPNQVRAELWTSP 183
           RLVD+G GQFKL+CLWKLRDFKC VELINRI   V++  HFPD+HL+QPNQV AELWTS 
Sbjct: 122 RLVDNGRGQFKLRCLWKLRDFKCCVELINRISNAVDDANHFPDIHLDQPNQVTAELWTSA 181

Query: 184 IGGLSMNDFIVAAKIDEIKTSDLAPRKRVWA 212
           IGGLSMNDFIVAAKIDEIKT DLA RKR WA
Sbjct: 182 IGGLSMNDFIVAAKIDEIKTIDLAARKRAWA 210

[Close]

BLAST of HG10009362 vs. TAIR 10
Match: AT5G51110.1 (Transcriptional coactivator/pterin dehydratase )

HSP 1 Score: 241.5 bits (615), Expect = 6.1e-64
Identity = 116/187 (62.03%), Postives = 140/187 (74.87%), Query Frame = 0


Query: 25  PPPTTLLRRNHRSASIIVSVGADKLGDFGARDPFPAEIESNFGEKVVGFSDTEHKILIPN 84
           PP +   R+  R      ++  D LGDFGARDP+P EI S FG+KV+G   TEHKILIPN
Sbjct: 34  PPVSRNNRKLGRLTVTRSNLAQDFLGDFGARDPYPEEIASQFGDKVLGCQSTEHKILIPN 93

Query: 85  VRALALSQQQCVPVSSLQTPMAEDEAQKLLKKVVGWRLVDDGTGQFKLQCLWKLRDFKCS 144
              L+LSQ QC PVSS Q P++ D+A+ LL KV+GW +VD+  G  K++C+WK+RDF C 
Sbjct: 94  ASVLSLSQLQCSPVSSSQPPLSGDDARTLLHKVLGWSIVDNEAGGLKIRCMWKVRDFGCG 153

Query: 145 VELINRIFKVVENVGHFPDLHLEQPNQVRAELWTSPIGGLSMNDFIVAAKIDEIKTSDLA 204
           VELINRI KV E  GH+P LHLE P QVRAEL+TS IGGLSMNDFI+AAKID+IKTSDL+
Sbjct: 154 VELINRIHKVAEASGHYPSLHLESPTQVRAELFTSSIGGLSMNDFIMAAKIDDIKTSDLS 213

Query: 205 PRKRVWA 212
           PRKR WA
Sbjct: 214 PRKRAWA 220

[Close]

BLAST of HG10009362 vs. TAIR 10
Match: AT5G51110.2 (Transcriptional coactivator/pterin dehydratase )

HSP 1 Score: 240.7 bits (613), Expect = 1.0e-63
Identity = 113/169 (66.86%), Postives = 134/169 (79.29%), Query Frame = 0


Query: 43  SVGADKLGDFGARDPFPAEIESNFGEKVVGFSDTEHKILIPNVRALALSQQQCVPVSSLQ 102
           S+  D LGDFGARDP+P EI S FG+KV+G   TEHKILIPN   L+LSQ QC PVSS Q
Sbjct: 25  SILKDFLGDFGARDPYPEEIASQFGDKVLGCQSTEHKILIPNASVLSLSQLQCSPVSSSQ 84

Query: 103 TPMAEDEAQKLLKKVVGWRLVDDGTGQFKLQCLWKLRDFKCSVELINRIFKVVENVGHFP 162
            P++ D+A+ LL KV+GW +VD+  G  K++C+WK+RDF C VELINRI KV E  GH+P
Sbjct: 85  PPLSGDDARTLLHKVLGWSIVDNEAGGLKIRCMWKVRDFGCGVELINRIHKVAEASGHYP 144

Query: 163 DLHLEQPNQVRAELWTSPIGGLSMNDFIVAAKIDEIKTSDLAPRKRVWA 212
            LHLE P QVRAEL+TS IGGLSMNDFI+AAKID+IKTSDL+PRKR WA
Sbjct: 145 SLHLESPTQVRAELFTSSIGGLSMNDFIMAAKIDDIKTSDLSPRKRAWA 193

[Close]

BLAST of HG10009362 vs. TAIR 10
Match: AT1G29810.1 (Transcriptional coactivator/pterin dehydratase )

HSP 1 Score: 105.5 bits (262), Expect = 5.2e-23
Identity = 52/121 (42.98%), Postives = 75/121 (61.98%), Query Frame = 0


Query: 90  LSQQQCVPVSSLQ-TPMAEDEAQKLLKKVVGWRLVDDGTGQFKLQCLWKLRDFKCSVELI 149
           LS ++CVP ++     M E  AQ LL+KV GW L +D     KL   W+++ F   ++  
Sbjct: 66  LSTKKCVPCNAKDLRAMTEQSAQDLLQKVAGWDLAND-NDTLKLHRSWRVKSFTKGLDFF 125

Query: 150 NRIFKVVENVGHFPDLHLEQPNQVRAELWTSPIGGLSMNDFIVAAKIDEIKTSDLAPRKR 209
            R+  + E+ GH PDLHL   N V+ E+WT  IGGL+ NDFI+AAKI+E++  DL  +K+
Sbjct: 126 QRVADIAESEGHHPDLHLVGWNNVKIEIWTHAIGGLTENDFILAAKINELQVEDLLRKKK 185

The following BLAST results are available for this feature:

BLAST of HG10009362 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038877111.1	7.6e-109	93.84	probable pterin-4-alpha-carbinolamine dehydratase, chloroplastic [Benincasa hisp...	[more]
XP_008436971.1	9.7e-96	84.58	PREDICTED: putative pterin-4-alpha-carbinolamine dehydratase [Cucumis melo] >KAA...	[more]
XP_004143676.1	2.2e-95	84.58	probable pterin-4-alpha-carbinolamine dehydratase, chloroplastic [Cucumis sativu...	[more]
XP_022156424.1	1.0e-84	77.10	uncharacterized protein LOC111023314 [Momordica charantia]	[more]
XP_022996033.1	2.2e-84	76.78	uncharacterized protein LOC111491359 [Cucurbita maxima] >XP_023532646.1 uncharac...	[more]

BLAST of HG10009362 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LU63	8.6e-63	62.03	Probable pterin-4-alpha-carbinolamine dehydratase, chloroplastic OS=Arabidopsis ...	[more]
Q6QJ72	7.3e-22	42.98	Pterin-4-alpha-carbinolamine dehydratase 2, mitochondrial OS=Arabidopsis thalian...	[more]
B0JQV0	3.1e-12	35.78	Putative pterin-4-alpha-carbinolamine dehydratase OS=Microcystis aeruginosa (str...	[more]
Q60BH8	4.5e-11	34.82	Putative pterin-4-alpha-carbinolamine dehydratase OS=Methylococcus capsulatus (s...	[more]
Q3B6N5	2.9e-10	33.03	Putative pterin-4-alpha-carbinolamine dehydratase OS=Pelodictyon luteolum (strai...	[more]

BLAST of HG10009362 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UGL0	4.7e-96	84.58	4a-hydroxytetrahydrobiopterin dehydratase OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3ASJ9	4.7e-96	84.58	4a-hydroxytetrahydrobiopterin dehydratase OS=Cucumis melo OX=3656 GN=LOC10348254...	[more]
A0A0A0KL10	1.0e-95	84.58	4a-hydroxytetrahydrobiopterin dehydratase OS=Cucumis sativus OX=3659 GN=Csa_5G16...	[more]
A0A6J1DTF1	4.8e-85	77.10	4a-hydroxytetrahydrobiopterin dehydratase OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1K9P8	1.1e-84	76.78	4a-hydroxytetrahydrobiopterin dehydratase OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]

BLAST of HG10009362 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G51110.1	6.1e-64	62.03	Transcriptional coactivator/pterin dehydratase	[more]
AT5G51110.2	1.0e-63	66.86	Transcriptional coactivator/pterin dehydratase	[more]
AT1G29810.1	5.2e-23	42.98	Transcriptional coactivator/pterin dehydratase	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036428	Pterin 4 alpha carbinolamine dehydratase superfamily	GENE3D	3.30.1360.20	Transcriptional coactivator/pterin dehydratase	coord: 104..203 e-value: 1.4E-20 score: 74.9
IPR036428	Pterin 4 alpha carbinolamine dehydratase superfamily	SUPERFAMILY	55248	PCD-like	coord: 103..198
IPR001533	Pterin 4 alpha carbinolamine dehydratase	PFAM	PF01329	Pterin_4a	coord: 104..198 e-value: 6.7E-21 score: 74.2
IPR001533	Pterin 4 alpha carbinolamine dehydratase	PANTHER	PTHR12599	PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE	coord: 35..211
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..29
None	No IPR available	PANTHER	PTHR12599:SF8	PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE, CHLOROPLASTIC-RELATED	coord: 35..211

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10009362.1	HG10009362.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009737	response to abscisic acid
biological_process	GO:0009651	response to salt stress
biological_process	GO:0110102	ribulose bisphosphate carboxylase complex assembly
biological_process	GO:0006729	tetrahydrobiopterin biosynthetic process
cellular_component	GO:0071944	cell periphery
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008124	4-alpha-hydroxytetrahydrobiopterin dehydratase activity