HG10009350 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10009350
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionE3 ubiquitin ligase
LocationChr06: 5010593 .. 5017852 (+)
RNA-Seq ExpressionHG10009350
SyntenyHG10009350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGACGAAGCCAATAGAAATCTTATGTTGCTGCCTCCCCCTTCTCCAGTTGTAGCCGTTGCTATCAGTGGGAAGAAAAACAGTAAATATATAATTAGGTGGTCATTGGAAAAGTTTCTACCTGAGGGCATCACTGATTTCAAGTTGCTACACATCATCCCAAGGATTACTAGTGTCCCAACTCCAAGTAAGCCATGCACTTCAAATTTATTGGTAAAGAGGGAATGTGTTTTTGGTCTTTTAAGTAGAATGTAAGGTTGCTATTGGCTAGGAAAATACTTTGAAATAAATATAGCCTTCTCTGCAACAATCAAGTGAAATACTAATTTTATTTGATAACGCTTAAAGAGTGACATGTGTAAAAAGTTATTGTTTTATCAAATTTTGATCATTTTTGTTCAGTTTAAGCTAGGTCCTTGCTGTGAAAATTGAAAAGGACACTTCTTTGACCATCTAGAGAATATCCATGAGTTTTCTCGCATATTTATGTTAATTTAATTCAAGCAGGGAATTCTGGGTAGCGGCCCCAATCTTTGCAGTAACCTTGAACAAAGTTGCATCATGAGATTGGTTATGAAATGATAGCAGGAAATGAGATCATGACCAAGTTACGTCAATCCTATACATTGTGTTAATTTTTGTTTGTTTGTTTTATTATTTCTTATGTGATTTGATTTTATGATGCTAATAACTAATATGTGGGGGGTGCACGTGCGAGACAAAATCTTCATTCTTCATTCTTCATTTGATGTAAAACCGGTCATCAAATTTTCCTTGTATGTTCTTGTGGCTAACGCTAGACTTTGCCTAGATTGCATATTCAAGCTATATGCCAATGTTGATGCAGTGGGAAATTCAATTCCAATTTCACAAGTTCGTGATGATGTTGCTGCGGCTTACAGGAAAGAAATCTGGTGGCAGACAAGTGAAAAACTTCTTCCATATAAGAAGATGTTTGCTCAGAGAAAGGTCCAGCCTTACTCTAATTTGAATTATGTGTAGGAAAACAGAATGAACTCAAATCTTCTTTCCAATTGTAGGTTCATCTTGATGTTGCAACTCTTGAAGCAGATGATGTGGCAAATGCAATAATAGAAGAGGTTACAAAGTCTTCAATCAACAAGCTTGTTATAGGAGTTTCATCACAGGGATTATTCTCAAGGTAATTAACTACCTTCTAGCATGATCTTACTGAATTATTTGAAATTTAACTTTATTAGCGGGTTGTCAATATTTCTCTTTATTGTTACACCTTAAGTCTATTTGACATTCGAAATAGGTTGTTCGTATATTCTATTTCTCATGCTGTGTTAGGTTATGAAGGGTTTTAAATTTCCAAATATCTATATATGAACTGAGCCTCAGCCATGTCCTGTTTAATTAGGATAGTTTGAGTGCATAGGAAAAGGTATTTCAATAAGGAAATAAAAAAAAAGAAGGTTGGATTATAGTTTATGATATCATATGCAGAATAACATATTTATAATTGGTTATTGAATTAATCTTTCATTATTTCCATTTTCTTGTGAGGGAAGTTAAGATATATAATGACAAATACCAGATCAAGGTTTCTGGAGGGTTGTGAAAGTTGTAAATAAGGTTGAAAGTGGAGACATCAAGAAGCATGTCCCTGATTTCTTGTTTTTATTAAATTATAAGCCATATGAATGACCCTCACATAGTCTACAACATCACTAAGTTATTACAATTGATTGGGGAAAGTAAGCTTAAAGGGACAACCAGTGCTTATTCGATCATTAAACTGATTTCTTCGATATTCCACTCATAACTTTGTCCTTGTCAATGTAATAAGGTTGGCCTTATACAGGTTTACTGAAACGAAAAGACAATAAATTTTCTCATTTGTAAAATTCATTATTAAAGAGATCTGTAATGAGTTCAGTGGGGTAGTTGAACTTACTTCCTGGATTTTTGCCTCCTATTGGATGCTTGCTGTACAGTTCACCCAGAACTGAAATTAATATATGCACGGAAACTTGTACAAACTAAACGACATAAATATAAGTGAAACGAGCCAAGGTTGCATGAAAGAAATGTTTTTGTTGCATGAAGAATTATAAATGAAATATGTACCAAAACATAGTATTACATTTATTTTCTCATGTTTTTTTGTTGTTAACTCTCGTGTCTGGATGACATTTTTATATAGGAAACTAAACGGTCTATCCTCAAGAATATCAGCCCTTGCACCTAGATATTGTACAGTCTATGCTATTTCAAAAGGAAAACTAGCCTCAATACGACCTCCTGATATGGAGACAAGCGTCAGCATTAAAGATGATGCGAGTGAAGCAAGTTCTGCAAATAGCTACTCGAGCTATTCATCTAGTTCCCTTACAGGTAACCATTAAGTAGTTAAGCTTTGATCTACATACATATTAGATGAAAGTAATATAACCAATTAAAGGATACTTGAAAGCTTGGTGGCTAATCATCAGTTCCATATTTAGAGGTTATACAATTGTATCTAAGGTTCACCAGCATTTAACCAGAGACAGGGTTCGTTTGTCTTTGGGTTATATACTTAACTACTGTTCTTATCATATTTACATTTTTTCACAACATCAAATATGTATTACGTATTCTTATTTTGTTGTACTTTTTATGATTTTTGATCTTCAAGTTTTTTTGTCCGCAAGGATTAGGTTTGTCGTTGTCGATTCTTAACTTATTATTTATAAGTAGTGGATAAGAGTGATGGAATTTCTGTCCATTACCTTCTCTGCCTTTTGTTAAAGATTCCAACTTTCATATCTTATAAAGTCTCCAACTCCCTGGTTTAACTAATTAAACTATACTGGTAAAAGATTGAATTAACATATGTTCAAACTTTCATACCTTACTATTCAGTTCTGTCTGTTTTCTTGATACAGATGCCAGTTCGAGCTTAATTACCTCCTACTCTCATTTCCCTTCTCCTTCCCCTTCCCTACCATTACAACGATTTCAAGCTCTTTCAACCATTAATCAATCTCTTCTTACAACAAAACCAAGCCCCATTAAAGCCAATCATTCTAGATGTCAATCCGTTGATATTGAGGATCAGGTGGATGGAGTTCGTTCTTCTTCCTATGTTTCAGATTGCGCACAAACATTGAGTCGAACCTCTAGTTGTAAGAGCTTGCCAACAGATAACCAGTCTTGGGTTTCTGATGAAGCTTCATCCTCAGGCGCGTTCAATGATTATTCTTCATCTGAAAGTCAGGTAATCCATCTGCTATTGTCCTTGACTTGTTAGTTCAATGGATCATTTCATGTGTCCTCTGGCTTGTTATCTTTTGGTCTCCTTGGCTTGAGCGAAATGGGAGGATCTTCACCGATAAATACTCATCCTTTGATAACTGCATGAATCTTGTATTATTATTTTAATGCTTTTTAGTGGTGTAAACATTTAGATCCATTCAAGGCTTTAAGACTTTCTACTTTATTTTCTTCTTGGAGAGCTTTTCTGTAATTCATCACTGGTGGTTGAGAGATTATGGCCTACTTGTCAAGTTCTTTTGACAAGGAGTGAAATAATATTAACTTAGTTATCTTAAATCAACGATTGACCAAAAAGCTTAAGCTAATAGGTGAAGGCAAATTTAATATAATATCTAACACTTCTCCTCATTTGCGGGCTTGAAATATGAAGAAGAGCTAAGCAAGTGGAAATGAATATTAATTGGGGAGGAAACAACAATGCAGGGGCTTGAACCTAGGACATCCTGAAGCAACCTGCTCTGATACCATCTTAAATCACCGATTGACCCAAAAGCTTAAGCTGATAGGTGAAGGCAAATTTAATATAATATTAACACATATCATGTTTATGTTAGAGATGGTTTTAGGAATCTTGGAAAGGAAAGATTGTATTGCAAACTTATACATTTGCATGTCAAGGTATGTGGAGATCTGGGATCATCTGCCATCATTCCTCATTGGCAGGAGGACCCAATTTCATGTGTGCTAGGCATTCACCCATTCATCAATTTTTTACATTGATAGTTTGACAATCCATGACTTCTATCTTCACATCATAAAATGTTAAGTGCTGATATAATATTATATTTACCTTCACCCATAAGCTTAAGCTTTTGGGTCAATCGGTAATTTAGCAAAGTATCAGAGTAGAATGTCTAGGAAGTCTTGTGTTCAAACTCCTACATGTTATTTGCTCCCCAATTAAAATTGATTTCCACTTGTTGGATCTTCTACAAATTTCTAAGCCCACAAGTAAGGAGTGTTAAATTTAACTTCACTCATAAACTTAAGCTTTTGAGTCAATCGGTTATTTAACATAAAGACTTCTAGGATACAACTTTCATTCTAACGTTCATTTATTTGATGTTACTGTGATATATTTGTATATTTAAGTTGTTCTCTCGCGTTGGCAGGCAGATGTCAGCTTTGAGCTTGAAAAGTTGAGAATCGAACTAAGACATGCGAGAGGAATGTATGCAATTGCTCAACGTGAGACGATTGATGCCTCTCGAAAGGTAGATGTCATATTTTTTTTATTTGTCATTTAGAACCTATGAATAATATCCTCTCCCCTGGTTTTTATTTCATAGTCAGATCAGAGATAGATGATTGATCTTAGCAAGATTATATCTTCATCAAGCATGACTATTTTGACAATCTCTCCTATGCTAAGTCTAGCTAACATATCAATATCAAGGGTTGCTCCTTTTTGTGGGGTTTTTTTTTTTGGTATGTACGTTATAATCTTTCATTTTTTTCTCAATGAAAGTTGGTCTTATATCAAAAAAGTCTTCTCGCAGTCTTATACCTCTTTCTTGTCTTAGATTCTAAAATTGAAAATAGAAGTAAACAAATATAAAGATAAATTATGGTCTAAATTGGATTGTACCAAACATGATTAAGAATGTTAAGTTGTTAACATATTGTTATTATTATTATTATTATTATTTTTTAACAGTAAACAAGCTGAAAAGTGAAATTCATATTAAAAGGAAAAATTGTTAAATTAGGAATTGTTTATTGTATACTCATTAAAACTTCTTTTTTGCATATTAATTGTAGTAAGTTGGATTTAATTTTTTGGACTCTTTTATTGTTTTCATTCTTCTTCGGCAGGATTAATATGTAAACTCATTTAACCAACTGCTTTTTCATTTGGAACAAGAACTTATAACAGAACATATTTTAATTAAGAGACAGTACAAATTATTTTGACTACATAACAATTAGCAAGAAAAGAAAAGTCACCTCGTTATTGTCTATGATCAGCTGAACCATCTAAATAAGCAACGATCAGACGAAGCTAGGAAGCTCGAAGAAATCAACAATAAAGAGGTGGCCGCCAAAGAATTTGCAATGGAAGAGAGAATGAAACGTGAAGCTTTGAGAAGAGAAGCAAAGTATGTAAAAGAATGCGCCGAAAGAGAGGGTATCTATAGGAAGGAAGCAGAGACAAAAGCTCTTCAGGATGCCAAAGAGAAGGGAAAGCATGAGAATGCTCTTCAAGGACCTTTGCAGCAATACCAACATTTTCAGTGGGAAGATATTGTTTCTGCCACATCATCTTTCTCTGAGGATCTTAAGATTGGAATGGGAGCACATGGAACAGTTTATAAGTGCAGTTTGCATCATACAACTGTAGCAGTGAAGGTTCTTCACTCTAGAGATGGTCACAAAAGGATGCAACTTCTTCAGGAGGTGAGTGAATTATTCAAAATGACATCTAGAAAACTTGTTGATTCTAGGATTTCATTACGTTTCGGATAAAAGAAAGTAGGCCTGTTCGTTATTTCCTTAATGCCATAGTCGAGGTCTTCTCGTACCTATTTGCCTATGTTCAACTGTCTCAAATAATTGCTTGAATCCATTGTATCATTATCCAAAATCAGCAGCATCTTTCACAACTAAGTTTTTGTGCTTTCTGAAATTTGCTTGTTTTTTTGTTATCATCATTCACCATCACAATTCAACATATAAATTCTCTGTCTCATATTGTATGATTATTCTAACTGAATCAATTTTTATATCTTCTGCTGACTTTCAGCTCGAAATCTTGAGCAGAATCCATCATCCCCATCTACTACTACTCCTCGGTGCGTGTCCGGATAAGAATTGTCTGGTTTATGAGTACATGGAAAATGGTAGCTTGGAGGATAGGTTGTACCGCAGGGGCAATACACCTGCAATCCCGTGGTATGAGAGGTTTCGAATTGCTTGGGAAATAGCCTCTGCTCTTGTCTTTCTTCACAGCTCAAAACCAAAACCAATAATCCACCGTGACCTTAAACCAGCAAACATCTTACTTGACCAAAACCTCGTGAGCAAAATCGGCGATGTCGGTCTTTCTACAGTGTTTAATTCAGACCCTTCAATGTCTACTGCGTTCATGAATAGTGGACCAGTTGGGACTCTCTGTTATATTGATCCTGAATATCAACGAACTGGATTAGTTTCACCTAAGTCTGATGTATATGCCTTTGGAATGGTGATCTTACAGTTACTAACTGCAAAACCAGCAGTAGCACTAACTCATGTGGTGGAAACAGCCATTGATAACTGCACTTTAATTAATGTTCTGGATATAGAGGCTGGGCATTGGCCTGTCGAAGAGACGTGTGAATTGGCAAGATTAGGACTTCGCTGCGCCGAGATGCAACGTAAAGATAGGCCTGACTTGAAGGATCAAGTACTTCCCTTGTTGATGACACTGAAAAAAGTTGCTCACGAGGCTCGCAACTTGGCCTCCAAAGTTCCAGCTGCAATTCCTAATCATTTCATCTGTCCGATTCTTCAGGTATGACTGTTGTTTTACAATATTTTTTATAGTTGGTGAACTTGCATCTAGAAAGTTTCGAAATGTAAGTTGAATAAGGATAATATAGGAAGGAATAAGGTGAACCTGACCATAACTCAACCAAGAGGTTAGAGGTTCAAATTCCTACCTCACGTGTTGCTGAACTCGAAACATAAAAACTTGTTAGGCTTATTCTTGCTGGAAATTCTGCTGATTGGTTTCTTAACATATATTTTCCCTGGTTGGTTTCTTAACATATATTTTCCCTGAAAGAACTGTGCTGAATTTCCCCACAGGACATGATGAATGACCCTTGTGTTGCGGCGGATGGATACACATACGATCGTCGGGCAATAGAGAAGTGGTTTCAGGAGAACGATAACTCACCGATGACGAAGTTGCCGCTGCCAGATAAGAATTTAATACCAAATTATAGTCTGCTCTCTGCAATTGTTGAGTGGAACTCCAGAAAAAATTAA

mRNA sequence

ATGGAAGACGAAGCCAATAGAAATCTTATGTTGCTGCCTCCCCCTTCTCCAGTTGTAGCCGTTGCTATCAGTGGGAAGAAAAACAGTAAATATATAATTAGGTGGTCATTGGAAAAGTTTCTACCTGAGGGCATCACTGATTTCAAGTTGCTACACATCATCCCAAGGATTACTAGTGTCCCAACTCCAATGGGAAATTCAATTCCAATTTCACAAGTTCGTGATGATGTTGCTGCGGCTTACAGGAAAGAAATCTGGTGGCAGACAAGTGAAAAACTTCTTCCATATAAGAAGATGTTTGCTCAGAGAAAGGTTCATCTTGATGTTGCAACTCTTGAAGCAGATGATGTGGCAAATGCAATAATAGAAGAGGTTACAAAGTCTTCAATCAACAAGCTTGTTATAGGAGTTTCATCACAGGGATTATTCTCAAGGAAACTAAACGGTCTATCCTCAAGAATATCAGCCCTTGCACCTAGATATTGTACAGTCTATGCTATTTCAAAAGGAAAACTAGCCTCAATACGACCTCCTGATATGGAGACAAGCGTCAGCATTAAAGATGATGCGAGTGAAGCAAGTTCTGCAAATAGCTACTCGAGCTATTCATCTAGTTCCCTTACAGATGCCAGTTCGAGCTTAATTACCTCCTACTCTCATTTCCCTTCTCCTTCCCCTTCCCTACCATTACAACGATTTCAAGCTCTTTCAACCATTAATCAATCTCTTCTTACAACAAAACCAAGCCCCATTAAAGCCAATCATTCTAGATGTCAATCCGTTGATATTGAGGATCAGGTGGATGGAGTTCGTTCTTCTTCCTATGTTTCAGATTGCGCACAAACATTGAGTCGAACCTCTAGTTGTAAGAGCTTGCCAACAGATAACCAGTCTTGGGTTTCTGATGAAGCTTCATCCTCAGGCGCGTTCAATGATTATTCTTCATCTGAAAGTCAGGCAGATGTCAGCTTTGAGCTTGAAAAGTTGAGAATCGAACTAAGACATGCGAGAGGAATGTATGCAATTGCTCAACGTGAGACGATTGATGCCTCTCGAAAGCAAGAAAAGAAAAGTCACCTCGTTATTGTCTATGATCAGCTGAACCATCTAAATAAGCAACGATCAGACGAAGCTAGGAAGCTCGAAGAAATCAACAATAAAGAGGTGGCCGCCAAAGAATTTGCAATGGAAGAGAGAATGAAACGTGAAGCTTTGAGAAGAGAAGCAAAGTATGTAAAAGAATGCGCCGAAAGAGAGGGTATCTATAGGAAGGAAGCAGAGACAAAAGCTCTTCAGGATGCCAAAGAGAAGGGAAAGCATGAGAATGCTCTTCAAGGACCTTTGCAGCAATACCAACATTTTCAGTGGGAAGATATTGTTTCTGCCACATCATCTTTCTCTGAGGATCTTAAGATTGGAATGGGAGCACATGGAACAGTTTATAAGTGCAGTTTGCATCATACAACTGTAGCAGTGAAGGTTCTTCACTCTAGAGATGGTCACAAAAGGATGCAACTTCTTCAGGAGCTCGAAATCTTGAGCAGAATCCATCATCCCCATCTACTACTACTCCTCGGTGCGTGTCCGGATAAGAATTGTCTGGTTTATGAGTACATGGAAAATGGTAGCTTGGAGGATAGGTTGTACCGCAGGGGCAATACACCTGCAATCCCGTGGTATGAGAGGTTTCGAATTGCTTGGGAAATAGCCTCTGCTCTTGTCTTTCTTCACAGCTCAAAACCAAAACCAATAATCCACCGTGACCTTAAACCAGCAAACATCTTACTTGACCAAAACCTCGTGAGCAAAATCGGCGATGTCGGTCTTTCTACAGTGTTTAATTCAGACCCTTCAATGTCTACTGCGTTCATGAATAGTGGACCAGTTGGGACTCTCTGTTATATTGATCCTGAATATCAACGAACTGGATTAGTTTCACCTAAGTCTGATGTATATGCCTTTGGAATGGTGATCTTACAGTTACTAACTGCAAAACCAGCAGTAGCACTAACTCATGTGGTGGAAACAGCCATTGATAACTGCACTTTAATTAATGTTCTGGATATAGAGGCTGGGCATTGGCCTGTCGAAGAGACGTGTGAATTGGCAAGATTAGGACTTCGCTGCGCCGAGATGCAACGTAAAGATAGGCCTGACTTGAAGGATCAAGTACTTCCCTTGTTGATGACACTGAAAAAAGTTGCTCACGAGGCTCGCAACTTGGCCTCCAAAGTTCCAGCTGCAATTCCTAATCATTTCATCTGTCCGATTCTTCAGGACATGATGAATGACCCTTGTGTTGCGGCGGATGGATACACATACGATCGTCGGGCAATAGAGAAGTGGTTTCAGGAGAACGATAACTCACCGATGACGAAGTTGCCGCTGCCAGATAAGAATTTAATACCAAATTATAGTCTGCTCTCTGCAATTGTTGAGTGGAACTCCAGAAAAAATTAA

Coding sequence (CDS)

ATGGAAGACGAAGCCAATAGAAATCTTATGTTGCTGCCTCCCCCTTCTCCAGTTGTAGCCGTTGCTATCAGTGGGAAGAAAAACAGTAAATATATAATTAGGTGGTCATTGGAAAAGTTTCTACCTGAGGGCATCACTGATTTCAAGTTGCTACACATCATCCCAAGGATTACTAGTGTCCCAACTCCAATGGGAAATTCAATTCCAATTTCACAAGTTCGTGATGATGTTGCTGCGGCTTACAGGAAAGAAATCTGGTGGCAGACAAGTGAAAAACTTCTTCCATATAAGAAGATGTTTGCTCAGAGAAAGGTTCATCTTGATGTTGCAACTCTTGAAGCAGATGATGTGGCAAATGCAATAATAGAAGAGGTTACAAAGTCTTCAATCAACAAGCTTGTTATAGGAGTTTCATCACAGGGATTATTCTCAAGGAAACTAAACGGTCTATCCTCAAGAATATCAGCCCTTGCACCTAGATATTGTACAGTCTATGCTATTTCAAAAGGAAAACTAGCCTCAATACGACCTCCTGATATGGAGACAAGCGTCAGCATTAAAGATGATGCGAGTGAAGCAAGTTCTGCAAATAGCTACTCGAGCTATTCATCTAGTTCCCTTACAGATGCCAGTTCGAGCTTAATTACCTCCTACTCTCATTTCCCTTCTCCTTCCCCTTCCCTACCATTACAACGATTTCAAGCTCTTTCAACCATTAATCAATCTCTTCTTACAACAAAACCAAGCCCCATTAAAGCCAATCATTCTAGATGTCAATCCGTTGATATTGAGGATCAGGTGGATGGAGTTCGTTCTTCTTCCTATGTTTCAGATTGCGCACAAACATTGAGTCGAACCTCTAGTTGTAAGAGCTTGCCAACAGATAACCAGTCTTGGGTTTCTGATGAAGCTTCATCCTCAGGCGCGTTCAATGATTATTCTTCATCTGAAAGTCAGGCAGATGTCAGCTTTGAGCTTGAAAAGTTGAGAATCGAACTAAGACATGCGAGAGGAATGTATGCAATTGCTCAACGTGAGACGATTGATGCCTCTCGAAAGCAAGAAAAGAAAAGTCACCTCGTTATTGTCTATGATCAGCTGAACCATCTAAATAAGCAACGATCAGACGAAGCTAGGAAGCTCGAAGAAATCAACAATAAAGAGGTGGCCGCCAAAGAATTTGCAATGGAAGAGAGAATGAAACGTGAAGCTTTGAGAAGAGAAGCAAAGTATGTAAAAGAATGCGCCGAAAGAGAGGGTATCTATAGGAAGGAAGCAGAGACAAAAGCTCTTCAGGATGCCAAAGAGAAGGGAAAGCATGAGAATGCTCTTCAAGGACCTTTGCAGCAATACCAACATTTTCAGTGGGAAGATATTGTTTCTGCCACATCATCTTTCTCTGAGGATCTTAAGATTGGAATGGGAGCACATGGAACAGTTTATAAGTGCAGTTTGCATCATACAACTGTAGCAGTGAAGGTTCTTCACTCTAGAGATGGTCACAAAAGGATGCAACTTCTTCAGGAGCTCGAAATCTTGAGCAGAATCCATCATCCCCATCTACTACTACTCCTCGGTGCGTGTCCGGATAAGAATTGTCTGGTTTATGAGTACATGGAAAATGGTAGCTTGGAGGATAGGTTGTACCGCAGGGGCAATACACCTGCAATCCCGTGGTATGAGAGGTTTCGAATTGCTTGGGAAATAGCCTCTGCTCTTGTCTTTCTTCACAGCTCAAAACCAAAACCAATAATCCACCGTGACCTTAAACCAGCAAACATCTTACTTGACCAAAACCTCGTGAGCAAAATCGGCGATGTCGGTCTTTCTACAGTGTTTAATTCAGACCCTTCAATGTCTACTGCGTTCATGAATAGTGGACCAGTTGGGACTCTCTGTTATATTGATCCTGAATATCAACGAACTGGATTAGTTTCACCTAAGTCTGATGTATATGCCTTTGGAATGGTGATCTTACAGTTACTAACTGCAAAACCAGCAGTAGCACTAACTCATGTGGTGGAAACAGCCATTGATAACTGCACTTTAATTAATGTTCTGGATATAGAGGCTGGGCATTGGCCTGTCGAAGAGACGTGTGAATTGGCAAGATTAGGACTTCGCTGCGCCGAGATGCAACGTAAAGATAGGCCTGACTTGAAGGATCAAGTACTTCCCTTGTTGATGACACTGAAAAAAGTTGCTCACGAGGCTCGCAACTTGGCCTCCAAAGTTCCAGCTGCAATTCCTAATCATTTCATCTGTCCGATTCTTCAGGACATGATGAATGACCCTTGTGTTGCGGCGGATGGATACACATACGATCGTCGGGCAATAGAGAAGTGGTTTCAGGAGAACGATAACTCACCGATGACGAAGTTGCCGCTGCCAGATAAGAATTTAATACCAAATTATAGTCTGCTCTCTGCAATTGTTGAGTGGAACTCCAGAAAAAATTAA

Protein sequence

MEDEANRNLMLLPPPSPVVAVAISGKKNSKYIIRWSLEKFLPEGITDFKLLHIIPRITSVPTPMGNSIPISQVRDDVAAAYRKEIWWQTSEKLLPYKKMFAQRKVHLDVATLEADDVANAIIEEVTKSSINKLVIGVSSQGLFSRKLNGLSSRISALAPRYCTVYAISKGKLASIRPPDMETSVSIKDDASEASSANSYSSYSSSSLTDASSSLITSYSHFPSPSPSLPLQRFQALSTINQSLLTTKPSPIKANHSRCQSVDIEDQVDGVRSSSYVSDCAQTLSRTSSCKSLPTDNQSWVSDEASSSGAFNDYSSSESQADVSFELEKLRIELRHARGMYAIAQRETIDASRKQEKKSHLVIVYDQLNHLNKQRSDEARKLEEINNKEVAAKEFAMEERMKREALRREAKYVKECAEREGIYRKEAETKALQDAKEKGKHENALQGPLQQYQHFQWEDIVSATSSFSEDLKIGMGAHGTVYKCSLHHTTVAVKVLHSRDGHKRMQLLQELEILSRIHHPHLLLLLGACPDKNCLVYEYMENGSLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDQNLVSKIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQRTGLVSPKSDVYAFGMVILQLLTAKPAVALTHVVETAIDNCTLINVLDIEAGHWPVEETCELARLGLRCAEMQRKDRPDLKDQVLPLLMTLKKVAHEARNLASKVPAAIPNHFICPILQDMMNDPCVAADGYTYDRRAIEKWFQENDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSRKN
Homology
BLAST of HG10009350 vs. NCBI nr
Match: XP_038877140.1 (U-box domain-containing protein 35-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1463.7 bits (3788), Expect = 0.0e+00
Identity = 760/819 (92.80%), Postives = 776/819 (94.75%), Query Frame = 0

Query: 1   MEDEANRNLMLLPPPSPVVAVAISGKKNSKYIIRWSLEKFLPEGITDFKLLHIIPRITSV 60
           MEDEANRNLMLLP PSPVVAVAISGKKNSKYIIRWSLEKFLP+GI  FKLLHIIPRITSV
Sbjct: 4   MEDEANRNLMLLPSPSPVVAVAISGKKNSKYIIRWSLEKFLPDGIIHFKLLHIIPRITSV 63

Query: 61  PTPMGNSIPISQVRDDVAAAYRKEIWWQTSEKLLPYKKMFAQRKVHLDVATLEADDVANA 120
           PTPMGNSIP+SQVR DVA AYRKEI WQTSEKLLP+KKMFAQRKVHLDV TLEADDVANA
Sbjct: 64  PTPMGNSIPVSQVRGDVAVAYRKEIGWQTSEKLLPFKKMFAQRKVHLDVVTLEADDVANA 123

Query: 121 IIEEVTKSSINKLVIGVSSQGLFSRKLNGLSSRISALAPRYCTVYAISKGKLASIRPPDM 180
           IIEEVTK SINKLVIGVSSQGLFSRKLN LSSRISALAPRYCT+YAISKGKLASIRPPDM
Sbjct: 124 IIEEVTKCSINKLVIGVSSQGLFSRKLNSLSSRISALAPRYCTIYAISKGKLASIRPPDM 183

Query: 181 ETSVSIKDDASEASSANSYSSYSSSSLTDASSSLITSYSHFPSPSPSLPLQRFQALSTIN 240
           ET+VSIKDDASE SSANSYSSY+SSSLTD SSSLITSYSHFPSPSPSLPLQRFQALST+N
Sbjct: 184 ETNVSIKDDASEGSSANSYSSYTSSSLTDGSSSLITSYSHFPSPSPSLPLQRFQALSTLN 243

Query: 241 QSLLTTKPSPIKANHSRCQSVDIEDQVDGVRSSSYVSDCAQTLSRTSSCKSLPTDNQSWV 300
           Q LLTTKPSPI+ANHSRCQS DI DQVD VRSSSYVSDCAQTLSR SSCKSL TDN SWV
Sbjct: 244 QPLLTTKPSPIEANHSRCQSNDIGDQVDRVRSSSYVSDCAQTLSRDSSCKSLSTDNLSWV 303

Query: 301 SDEASSSGAFNDYSSSESQADVSFELEKLRIELRHARGMYAIAQRETIDASRKQEKKSHL 360
           SDEASSSGAFNDY SSESQADVSFELEKLRIELRHARGMYAIAQRETIDASRK       
Sbjct: 304 SDEASSSGAFNDYYSSESQADVSFELEKLRIELRHARGMYAIAQRETIDASRK------- 363

Query: 361 VIVYDQLNHLNKQRSDEARKLEEINNKEVAAKEFAMEERMKREALRREAKYVKECAEREG 420
                 LNHLNKQRS+EARKLEEINNKEVAAKEFA EER+K EALRREAKYVKE AEREG
Sbjct: 364 ------LNHLNKQRSEEARKLEEINNKEVAAKEFAREERVKHEALRREAKYVKERAEREG 423

Query: 421 IYRKEAETKALQDAKEKGKHENALQGPLQQYQHFQWEDIVSATSSFSEDLKIGMGAHGTV 480
           IYRKEAETKALQDAKEKGKHENALQGPLQQYQ FQWEDIVSATSSFSEDLKIGMGAHG+V
Sbjct: 424 IYRKEAETKALQDAKEKGKHENALQGPLQQYQLFQWEDIVSATSSFSEDLKIGMGAHGSV 483

Query: 481 YKCSLHHTTVAVKVLHSRDGHKRMQLLQELEILSRIHHPHLLLLLGACPDKNCLVYEYME 540
           YKCSLHHTTVAVKVLHSRDGHKRMQLLQELEILSRIHHPHLLLLLGACPDKNCLVYEYME
Sbjct: 484 YKCSLHHTTVAVKVLHSRDGHKRMQLLQELEILSRIHHPHLLLLLGACPDKNCLVYEYME 543

Query: 541 NGSLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDQNL 600
           NGSLEDRLYRRG TPAIPWYERFRIAWE+ASALVFLHSSKPKPIIHRDLKPANILLD NL
Sbjct: 544 NGSLEDRLYRRGKTPAIPWYERFRIAWELASALVFLHSSKPKPIIHRDLKPANILLDHNL 603

Query: 601 VSKIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQRTGLVSPKSDVYAFGMVILQ 660
           VSKIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQR+GLVSPKSDVYAFGMVILQ
Sbjct: 604 VSKIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQRSGLVSPKSDVYAFGMVILQ 663

Query: 661 LLTAKPAVALTHVVETAIDNCTLINVLDIEAGHWPVEETCELARLGLRCAEMQRKDRPDL 720
           LLTAKPAVALTHVVETAIDNCTLI VLDIEAGHWPVEET ELARLGLRCAEMQRKDRPDL
Sbjct: 664 LLTAKPAVALTHVVETAIDNCTLIKVLDIEAGHWPVEETYELARLGLRCAEMQRKDRPDL 723

Query: 721 KDQVLPLLMTLKKVAHEARNLASKVPAAIPNHFICPILQDMMNDPCVAADGYTYDRRAIE 780
           KDQVLPLLMTLKKVA EARNL SKVPAAIPNHFICPILQD+MNDPCVAADGYTYDRRAIE
Sbjct: 724 KDQVLPLLMTLKKVADEARNLPSKVPAAIPNHFICPILQDVMNDPCVAADGYTYDRRAIE 783

Query: 781 KWFQENDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSRKN 820
           KW QENDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSR++
Sbjct: 784 KWLQENDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSRRS 809

BLAST of HG10009350 vs. NCBI nr
Match: XP_038877139.1 (U-box domain-containing protein 35-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1453.3 bits (3761), Expect = 0.0e+00
Identity = 759/831 (91.34%), Postives = 776/831 (93.38%), Query Frame = 0

Query: 1   MEDEANRNLMLLPPPSPVVAVAISGKKNSKYIIRWSLEKFLPEGITDFKLLHIIPRITSV 60
           MEDEANRNLMLLP PSPVVAVAISGKKNSKYIIRWSLEKFLP+GI  FKLLHIIPRITSV
Sbjct: 4   MEDEANRNLMLLPSPSPVVAVAISGKKNSKYIIRWSLEKFLPDGIIHFKLLHIIPRITSV 63

Query: 61  PTP------------MGNSIPISQVRDDVAAAYRKEIWWQTSEKLLPYKKMFAQRKVHLD 120
           PTP            +GNSIP+SQVR DVA AYRKEI WQTSEKLLP+KKMFAQRKVHLD
Sbjct: 64  PTPNCIFKLYANVDAVGNSIPVSQVRGDVAVAYRKEIGWQTSEKLLPFKKMFAQRKVHLD 123

Query: 121 VATLEADDVANAIIEEVTKSSINKLVIGVSSQGLFSRKLNGLSSRISALAPRYCTVYAIS 180
           V TLEADDVANAIIEEVTK SINKLVIGVSSQGLFSRKLN LSSRISALAPRYCT+YAIS
Sbjct: 124 VVTLEADDVANAIIEEVTKCSINKLVIGVSSQGLFSRKLNSLSSRISALAPRYCTIYAIS 183

Query: 181 KGKLASIRPPDMETSVSIKDDASEASSANSYSSYSSSSLTDASSSLITSYSHFPSPSPSL 240
           KGKLASIRPPDMET+VSIKDDASE SSANSYSSY+SSSLTD SSSLITSYSHFPSPSPSL
Sbjct: 184 KGKLASIRPPDMETNVSIKDDASEGSSANSYSSYTSSSLTDGSSSLITSYSHFPSPSPSL 243

Query: 241 PLQRFQALSTINQSLLTTKPSPIKANHSRCQSVDIEDQVDGVRSSSYVSDCAQTLSRTSS 300
           PLQRFQALST+NQ LLTTKPSPI+ANHSRCQS DI DQVD VRSSSYVSDCAQTLSR SS
Sbjct: 244 PLQRFQALSTLNQPLLTTKPSPIEANHSRCQSNDIGDQVDRVRSSSYVSDCAQTLSRDSS 303

Query: 301 CKSLPTDNQSWVSDEASSSGAFNDYSSSESQADVSFELEKLRIELRHARGMYAIAQRETI 360
           CKSL TDN SWVSDEASSSGAFNDY SSESQADVSFELEKLRIELRHARGMYAIAQRETI
Sbjct: 304 CKSLSTDNLSWVSDEASSSGAFNDYYSSESQADVSFELEKLRIELRHARGMYAIAQRETI 363

Query: 361 DASRKQEKKSHLVIVYDQLNHLNKQRSDEARKLEEINNKEVAAKEFAMEERMKREALRRE 420
           DASRK             LNHLNKQRS+EARKLEEINNKEVAAKEFA EER+K EALRRE
Sbjct: 364 DASRK-------------LNHLNKQRSEEARKLEEINNKEVAAKEFAREERVKHEALRRE 423

Query: 421 AKYVKECAEREGIYRKEAETKALQDAKEKGKHENALQGPLQQYQHFQWEDIVSATSSFSE 480
           AKYVKE AEREGIYRKEAETKALQDAKEKGKHENALQGPLQQYQ FQWEDIVSATSSFSE
Sbjct: 424 AKYVKERAEREGIYRKEAETKALQDAKEKGKHENALQGPLQQYQLFQWEDIVSATSSFSE 483

Query: 481 DLKIGMGAHGTVYKCSLHHTTVAVKVLHSRDGHKRMQLLQELEILSRIHHPHLLLLLGAC 540
           DLKIGMGAHG+VYKCSLHHTTVAVKVLHSRDGHKRMQLLQELEILSRIHHPHLLLLLGAC
Sbjct: 484 DLKIGMGAHGSVYKCSLHHTTVAVKVLHSRDGHKRMQLLQELEILSRIHHPHLLLLLGAC 543

Query: 541 PDKNCLVYEYMENGSLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPKPIIHRD 600
           PDKNCLVYEYMENGSLEDRLYRRG TPAIPWYERFRIAWE+ASALVFLHSSKPKPIIHRD
Sbjct: 544 PDKNCLVYEYMENGSLEDRLYRRGKTPAIPWYERFRIAWELASALVFLHSSKPKPIIHRD 603

Query: 601 LKPANILLDQNLVSKIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQRTGLVSPK 660
           LKPANILLD NLVSKIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQR+GLVSPK
Sbjct: 604 LKPANILLDHNLVSKIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQRSGLVSPK 663

Query: 661 SDVYAFGMVILQLLTAKPAVALTHVVETAIDNCTLINVLDIEAGHWPVEETCELARLGLR 720
           SDVYAFGMVILQLLTAKPAVALTHVVETAIDNCTLI VLDIEAGHWPVEET ELARLGLR
Sbjct: 664 SDVYAFGMVILQLLTAKPAVALTHVVETAIDNCTLIKVLDIEAGHWPVEETYELARLGLR 723

Query: 721 CAEMQRKDRPDLKDQVLPLLMTLKKVAHEARNLASKVPAAIPNHFICPILQDMMNDPCVA 780
           CAEMQRKDRPDLKDQVLPLLMTLKKVA EARNL SKVPAAIPNHFICPILQD+MNDPCVA
Sbjct: 724 CAEMQRKDRPDLKDQVLPLLMTLKKVADEARNLPSKVPAAIPNHFICPILQDVMNDPCVA 783

Query: 781 ADGYTYDRRAIEKWFQENDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSRKN 820
           ADGYTYDRRAIEKW QENDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSR++
Sbjct: 784 ADGYTYDRRAIEKWLQENDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSRRS 821

BLAST of HG10009350 vs. NCBI nr
Match: XP_008437017.1 (PREDICTED: U-box domain-containing protein 35-like isoform X2 [Cucumis melo])

HSP 1 Score: 1419.8 bits (3674), Expect = 0.0e+00
Identity = 734/817 (89.84%), Postives = 765/817 (93.64%), Query Frame = 0

Query: 3   DEANRNLMLLPPPSPVVAVAISGKKNSKYIIRWSLEKFLPEGITDFKLLHIIPRITSVPT 62
           D+A +N MLLP  SPVVAVAISGKKNSKYIIRWSLEKFLPEGI DFKLLH  PRITSVPT
Sbjct: 6   DQAKKNHMLLPSSSPVVAVAISGKKNSKYIIRWSLEKFLPEGIIDFKLLHFFPRITSVPT 65

Query: 63  PMGNSIPISQVRDDVAAAYRKEIWWQTSEKLLPYKKMFAQRKVHLDVATLEADDVANAII 122
           PMGN+IP+SQVR+DVA AYRKEIWWQTSEKLLP+KKMFAQRKVH+DV TLEADDV +AII
Sbjct: 66  PMGNAIPVSQVREDVAVAYRKEIWWQTSEKLLPFKKMFAQRKVHVDVVTLEADDVVDAII 125

Query: 123 EEVTKSSINKLVIGVSSQGLFSRKLNGLSSRISALAPRYCTVYAISKGKLASIRPPDMET 182
           EEVTK SINKLVIGVSSQGLFSRKL+GLSSRISALAPRYCTVYAISKGKLASIRPPDM+T
Sbjct: 126 EEVTKCSINKLVIGVSSQGLFSRKLSGLSSRISALAPRYCTVYAISKGKLASIRPPDMDT 185

Query: 183 SVSIKDDASEASSANSYSSYSSSSLTDASSSLITSYSHFPSPSPSLPLQRFQALSTINQS 242
            VSI+DDASE SSA+SYSSY+SSSLTD SSSL TSYSHFPSPSPSLPLQRFQALSTINQ 
Sbjct: 186 DVSIRDDASEESSASSYSSYTSSSLTDGSSSLTTSYSHFPSPSPSLPLQRFQALSTINQP 245

Query: 243 LLTTKPSPIKANHSRCQSVDIEDQVDGVRSSSYVSDCAQTLSRTSSCKSLPTDNQSWVSD 302
           LLT KPS IKA+HSRCQS+DIE  VDGVRSSS+VSDC QTLSR SS KS PT+NQSW+SD
Sbjct: 246 LLTKKPSLIKADHSRCQSIDIE-VVDGVRSSSHVSDCTQTLSRASSSKSSPTENQSWISD 305

Query: 303 EASSSGAFNDYSSSESQADVSFELEKLRIELRHARGMYAIAQRETIDASRKQEKKSHLVI 362
           EASSSGAFNDYSS ESQADVSFELEKLRIELRHARGMYAIAQRETIDASR          
Sbjct: 306 EASSSGAFNDYSSCESQADVSFELEKLRIELRHARGMYAIAQRETIDASR---------- 365

Query: 363 VYDQLNHLNKQRSDEARKLEEINNKEVAAKEFAMEERMKREALRREAKYVKECAEREGIY 422
              +LNHLNKQRS+EARKLEEINNK VAAKEFA EER+K EALRREAKYVKE AEREGIY
Sbjct: 366 ---ELNHLNKQRSEEARKLEEINNKVVAAKEFAREERVKHEALRREAKYVKERAEREGIY 425

Query: 423 RKEAETKALQDAKEKGKHENALQGPLQQYQHFQWEDIVSATSSFSEDLKIGMGAHGTVYK 482
           RKEAETKALQDAKEKGKHENALQGPLQQYQHFQWEDIVSAT SFSEDLKIGMGAHGTVYK
Sbjct: 426 RKEAETKALQDAKEKGKHENALQGPLQQYQHFQWEDIVSATLSFSEDLKIGMGAHGTVYK 485

Query: 483 CSLHHTTVAVKVLHSRDGHKRMQLLQELEILSRIHHPHLLLLLGACPDKNCLVYEYMENG 542
           CSLHHTTVAVKVLHSRD HK+MQLLQELE+LSRIHHPHLLLLLGACPDKNCLVYEYMENG
Sbjct: 486 CSLHHTTVAVKVLHSRDSHKQMQLLQELEVLSRIHHPHLLLLLGACPDKNCLVYEYMENG 545

Query: 543 SLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDQNLVS 602
           SLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPK IIHRDLKPANILLDQNLVS
Sbjct: 546 SLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPKSIIHRDLKPANILLDQNLVS 605

Query: 603 KIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQRTGLVSPKSDVYAFGMVILQLL 662
           KIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQRTGL+SPKSDVYAFGMVILQLL
Sbjct: 606 KIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQLL 665

Query: 663 TAKPAVALTHVVETAIDNCTLINVLDIEAGHWPVEETCELARLGLRCAEMQRKDRPDLKD 722
           TAKPAVALTHVVETAIDN  LI VLDIEAGHWP+EET ELARLGLRCAEMQRKDRPDLKD
Sbjct: 666 TAKPAVALTHVVETAIDNSNLIKVLDIEAGHWPLEETYELARLGLRCAEMQRKDRPDLKD 725

Query: 723 QVLPLLMTLKKVAHEARNLASKVPAAIPNHFICPILQDMMNDPCVAADGYTYDRRAIEKW 782
           QVLPLLMTLKKVA EARN ASKVPAAIPNHFICPILQD+MNDPCVAADGYTYDR+AIEKW
Sbjct: 726 QVLPLLMTLKKVADEARNFASKVPAAIPNHFICPILQDVMNDPCVAADGYTYDRQAIEKW 785

Query: 783 FQENDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSRKN 820
            Q+NDNSPMTKLPLPDKNLIPNYSLLSAIVEWNS+++
Sbjct: 786 LQKNDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSKRS 808

BLAST of HG10009350 vs. NCBI nr
Match: XP_011654848.1 (U-box domain-containing protein 35 isoform X3 [Cucumis sativus] >KGN49786.2 hypothetical protein Csa_000366 [Cucumis sativus])

HSP 1 Score: 1416.4 bits (3665), Expect = 0.0e+00
Identity = 734/817 (89.84%), Postives = 766/817 (93.76%), Query Frame = 0

Query: 3   DEANRNLMLLPPPSPVVAVAISGKKNSKYIIRWSLEKFLPEGITDFKLLHIIPRITSVPT 62
           D+A RN MLL P S VVAVAISGKKNSKYIIRWSLEKFLPEGI DF+LLH IPRITSVPT
Sbjct: 6   DQAKRNHMLL-PSSSVVAVAISGKKNSKYIIRWSLEKFLPEGIIDFRLLHFIPRITSVPT 65

Query: 63  PMGNSIPISQVRDDVAAAYRKEIWWQTSEKLLPYKKMFAQRKVHLDVATLEADDVANAII 122
           PMGN+IPISQVR+DVAAAYRKEIWW TSEKLLP+KKMFAQRKVHLDV TLEADDVA AII
Sbjct: 66  PMGNAIPISQVREDVAAAYRKEIWWHTSEKLLPFKKMFAQRKVHLDVVTLEADDVAGAII 125

Query: 123 EEVTKSSINKLVIGVSSQGLFSRKLNGLSSRISALAPRYCTVYAISKGKLASIRPPDMET 182
           EEVTK SINKLVIGVSSQGLFSRKL+GLSSRISALAPRYCTVYAISKGKLASIRPPDM+T
Sbjct: 126 EEVTKCSINKLVIGVSSQGLFSRKLSGLSSRISALAPRYCTVYAISKGKLASIRPPDMDT 185

Query: 183 SVSIKDDASEASSANSYSSYSSSSLTDASSSLITSYSHFPSPSPSLPLQRFQALSTINQS 242
           +VSI+DDASE SSA+SYSSY+SSSLTD SSSL +SYSHFPSPSPSLPLQRFQALSTINQ 
Sbjct: 186 NVSIRDDASEESSASSYSSYTSSSLTDGSSSLTSSYSHFPSPSPSLPLQRFQALSTINQP 245

Query: 243 LLTTKPSPIKANHSRCQSVDIEDQVDGVRSSSYVSDCAQTLSRTSSCKSLPTDNQSWVSD 302
           LLT KPSPIKA+HSRCQSVDIE+QVDGV SSSYVSDC QTLSR SS KS P +N+SW SD
Sbjct: 246 LLTKKPSPIKADHSRCQSVDIENQVDGVHSSSYVSDCIQTLSRASSSKSSPAENKSWNSD 305

Query: 303 EASSSGAFNDYSSSESQADVSFELEKLRIELRHARGMYAIAQRETIDASRKQEKKSHLVI 362
           EASSSG FNDYSS ESQADVSFELEKLRIELRHARGM+AIAQRETIDASR          
Sbjct: 306 EASSSGMFNDYSSCESQADVSFELEKLRIELRHARGMFAIAQRETIDASR---------- 365

Query: 363 VYDQLNHLNKQRSDEARKLEEINNKEVAAKEFAMEERMKREALRREAKYVKECAEREGIY 422
              +LNHLN QRS+EARKLEEINNK VAAKEFA EER+K EALRREAKYVKE AEREGIY
Sbjct: 366 ---ELNHLNNQRSEEARKLEEINNKAVAAKEFAREERVKHEALRREAKYVKERAEREGIY 425

Query: 423 RKEAETKALQDAKEKGKHENALQGPLQQYQHFQWEDIVSATSSFSEDLKIGMGAHGTVYK 482
           RKEAE KALQ+AKEKGKHENALQGPLQQYQHFQWEDIVSATSSFSEDLKIGMGAHGTVYK
Sbjct: 426 RKEAEMKALQNAKEKGKHENALQGPLQQYQHFQWEDIVSATSSFSEDLKIGMGAHGTVYK 485

Query: 483 CSLHHTTVAVKVLHSRDGHKRMQLLQELEILSRIHHPHLLLLLGACPDKNCLVYEYMENG 542
           CSLHHTTVAVKVLHSRD HK+MQLLQELE+LSRIHHPHLLLLLGACPDKNCLVYEYMENG
Sbjct: 486 CSLHHTTVAVKVLHSRDSHKQMQLLQELEVLSRIHHPHLLLLLGACPDKNCLVYEYMENG 545

Query: 543 SLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDQNLVS 602
           SLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPK IIHRDLKPANILLDQNLVS
Sbjct: 546 SLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPKSIIHRDLKPANILLDQNLVS 605

Query: 603 KIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQRTGLVSPKSDVYAFGMVILQLL 662
           KIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQRTGL+SPKSDVYAFGMVILQLL
Sbjct: 606 KIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQLL 665

Query: 663 TAKPAVALTHVVETAIDNCTLINVLDIEAGHWPVEETCELARLGLRCAEMQRKDRPDLKD 722
           TAKPAVALTHVVETAIDN  LINVLDIEAGHWP+EET ELARLGLRCAEMQRKDRPDLKD
Sbjct: 666 TAKPAVALTHVVETAIDNSNLINVLDIEAGHWPLEETYELARLGLRCAEMQRKDRPDLKD 725

Query: 723 QVLPLLMTLKKVAHEARNLASKVPAAIPNHFICPILQDMMNDPCVAADGYTYDRRAIEKW 782
           QVLPLLMTLKKVA +ARNLASKVPAAIPNHFICPILQD+MNDPCVAADGYTYDR+AIEKW
Sbjct: 726 QVLPLLMTLKKVADKARNLASKVPAAIPNHFICPILQDVMNDPCVAADGYTYDRQAIEKW 785

Query: 783 FQENDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSRKN 820
            Q+NDNSPMTKLPLPDKNLIPNYSLLSAIVEWNS+++
Sbjct: 786 LQKNDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSKRS 808

BLAST of HG10009350 vs. NCBI nr
Match: XP_011654847.1 (U-box domain-containing protein 35 isoform X2 [Cucumis sativus])

HSP 1 Score: 1409.8 bits (3648), Expect = 0.0e+00
Identity = 733/820 (89.39%), Postives = 766/820 (93.41%), Query Frame = 0

Query: 3   DEANRNLMLLPPPSPVVAVAISGKKNSKYIIRWSLEKFLPEGITDFKLLHIIPRITSVPT 62
           D+A RN MLL P S VVAVAISGKKNSKYIIRWSLEKFLPEGI DF+LLH IPRITSVPT
Sbjct: 6   DQAKRNHMLL-PSSSVVAVAISGKKNSKYIIRWSLEKFLPEGIIDFRLLHFIPRITSVPT 65

Query: 63  ---PMGNSIPISQVRDDVAAAYRKEIWWQTSEKLLPYKKMFAQRKVHLDVATLEADDVAN 122
              P+GN+IPISQVR+DVAAAYRKEIWW TSEKLLP+KKMFAQRKVHLDV TLEADDVA 
Sbjct: 66  PSKPLGNAIPISQVREDVAAAYRKEIWWHTSEKLLPFKKMFAQRKVHLDVVTLEADDVAG 125

Query: 123 AIIEEVTKSSINKLVIGVSSQGLFSRKLNGLSSRISALAPRYCTVYAISKGKLASIRPPD 182
           AIIEEVTK SINKLVIGVSSQGLFSRKL+GLSSRISALAPRYCTVYAISKGKLASIRPPD
Sbjct: 126 AIIEEVTKCSINKLVIGVSSQGLFSRKLSGLSSRISALAPRYCTVYAISKGKLASIRPPD 185

Query: 183 METSVSIKDDASEASSANSYSSYSSSSLTDASSSLITSYSHFPSPSPSLPLQRFQALSTI 242
           M+T+VSI+DDASE SSA+SYSSY+SSSLTD SSSL +SYSHFPSPSPSLPLQRFQALSTI
Sbjct: 186 MDTNVSIRDDASEESSASSYSSYTSSSLTDGSSSLTSSYSHFPSPSPSLPLQRFQALSTI 245

Query: 243 NQSLLTTKPSPIKANHSRCQSVDIEDQVDGVRSSSYVSDCAQTLSRTSSCKSLPTDNQSW 302
           NQ LLT KPSPIKA+HSRCQSVDIE+QVDGV SSSYVSDC QTLSR SS KS P +N+SW
Sbjct: 246 NQPLLTKKPSPIKADHSRCQSVDIENQVDGVHSSSYVSDCIQTLSRASSSKSSPAENKSW 305

Query: 303 VSDEASSSGAFNDYSSSESQADVSFELEKLRIELRHARGMYAIAQRETIDASRKQEKKSH 362
            SDEASSSG FNDYSS ESQADVSFELEKLRIELRHARGM+AIAQRETIDASR       
Sbjct: 306 NSDEASSSGMFNDYSSCESQADVSFELEKLRIELRHARGMFAIAQRETIDASR------- 365

Query: 363 LVIVYDQLNHLNKQRSDEARKLEEINNKEVAAKEFAMEERMKREALRREAKYVKECAERE 422
                 +LNHLN QRS+EARKLEEINNK VAAKEFA EER+K EALRREAKYVKE AERE
Sbjct: 366 ------ELNHLNNQRSEEARKLEEINNKAVAAKEFAREERVKHEALRREAKYVKERAERE 425

Query: 423 GIYRKEAETKALQDAKEKGKHENALQGPLQQYQHFQWEDIVSATSSFSEDLKIGMGAHGT 482
           GIYRKEAE KALQ+AKEKGKHENALQGPLQQYQHFQWEDIVSATSSFSEDLKIGMGAHGT
Sbjct: 426 GIYRKEAEMKALQNAKEKGKHENALQGPLQQYQHFQWEDIVSATSSFSEDLKIGMGAHGT 485

Query: 483 VYKCSLHHTTVAVKVLHSRDGHKRMQLLQELEILSRIHHPHLLLLLGACPDKNCLVYEYM 542
           VYKCSLHHTTVAVKVLHSRD HK+MQLLQELE+LSRIHHPHLLLLLGACPDKNCLVYEYM
Sbjct: 486 VYKCSLHHTTVAVKVLHSRDSHKQMQLLQELEVLSRIHHPHLLLLLGACPDKNCLVYEYM 545

Query: 543 ENGSLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDQN 602
           ENGSLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPK IIHRDLKPANILLDQN
Sbjct: 546 ENGSLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPKSIIHRDLKPANILLDQN 605

Query: 603 LVSKIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQRTGLVSPKSDVYAFGMVIL 662
           LVSKIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQRTGL+SPKSDVYAFGMVIL
Sbjct: 606 LVSKIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQRTGLISPKSDVYAFGMVIL 665

Query: 663 QLLTAKPAVALTHVVETAIDNCTLINVLDIEAGHWPVEETCELARLGLRCAEMQRKDRPD 722
           QLLTAKPAVALTHVVETAIDN  LINVLDIEAGHWP+EET ELARLGLRCAEMQRKDRPD
Sbjct: 666 QLLTAKPAVALTHVVETAIDNSNLINVLDIEAGHWPLEETYELARLGLRCAEMQRKDRPD 725

Query: 723 LKDQVLPLLMTLKKVAHEARNLASKVPAAIPNHFICPILQDMMNDPCVAADGYTYDRRAI 782
           LKDQVLPLLMTLKKVA +ARNLASKVPAAIPNHFICPILQD+MNDPCVAADGYTYDR+AI
Sbjct: 726 LKDQVLPLLMTLKKVADKARNLASKVPAAIPNHFICPILQDVMNDPCVAADGYTYDRQAI 785

Query: 783 EKWFQENDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSRKN 820
           EKW Q+NDNSPMTKLPLPDKNLIPNYSLLSAIVEWNS+++
Sbjct: 786 EKWLQKNDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSKRS 811

BLAST of HG10009350 vs. ExPASy Swiss-Prot
Match: Q9SW11 (U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2 SV=2)

HSP 1 Score: 819.3 bits (2115), Expect = 3.9e-236
Identity = 451/838 (53.82%), Postives = 584/838 (69.69%), Query Frame = 0

Query: 13  PPPSPVVAVAISGKKNSKYIIRWSLEKFLPEGITDFKLLHIIPRITSVPTPMGNSIPISQ 72
           PPPS  V VA+SG   SKY++ W++EKF  EG   FKLLHI P ITSVPTPMGN+IPIS+
Sbjct: 16  PPPSRTVVVALSGSSKSKYVVTWAIEKFATEGNVGFKLLHIHPMITSVPTPMGNAIPISE 75

Query: 73  VRDDVAAAYRKEIWWQTSEKLLPYKKMFAQRKVHLDVATLEADDVANAIIEEVTKSSINK 132
           VRDDV  AYR+EI WQ+ E L PY K+F +RKV ++V  +E+D+VA AI EEVT+ SI++
Sbjct: 76  VRDDVVTAYRQEILWQSEEMLKPYTKLFVRRKVAVEVLVIESDNVAAAIAEEVTRDSIDR 135

Query: 133 LVIGVSSQGLFSRKLNGLSSRISALAPRYCTVYAISKGKLASIRPPDMETSVSIKDDASE 192
           +VIG SS+  FSRK + + S ISAL P +CTVY +SKGKL+ +RP D + + +I++D SE
Sbjct: 136 IVIGGSSRSFFSRKAD-ICSVISALMPNFCTVYVVSKGKLSCVRPSDSDGNATIREDGSE 195

Query: 193 ASSANSYSSYSSSSLTDASSSLITSYSHFPSPSPSLPLQRFQALSTI-NQSLLTTKPSPI 252
            ++++S SS  +S  +D  SS   S S       SLP++R Q    I  Q+ +  + S +
Sbjct: 196 RTNSSSGSSGPTSDSSDVMSSAHDSQSR----PLSLPVRRMQHFPAIAGQASVPMETSSV 255

Query: 253 KANHSRCQSVDIED--QVDGVRSSSYVSDCAQTLSR-----TSSCKSLPTDNQ------- 312
            ++ +RC S+D E+   V  +  SS  +    T  R          S  + N+       
Sbjct: 256 GSDETRCMSLDAEEARDVSSINRSSTDTTSRWTPRRRDYEERKEAMSSSSSNREYGNFGT 315

Query: 313 --SW-----------VSDEASS-SGAFNDYSSSESQADVSFELEKLRIELRHARGMYAIA 372
             SW            S +AS+ S A ++ S +++Q +++FE+EKLR ELRH + MYA+A
Sbjct: 316 RFSWSGMGVDTTHSRASQQASNMSDALSEQSYTDNQVNLNFEVEKLRAELRHVQEMYAVA 375

Query: 373 QRETIDASRKQEKKSHLVIVYDQLNHLNKQRSDEARKLEEINNKEVAAKEFAMEERMKRE 432
           Q ET DASRK             L  LN++R +EA KLEE+  KE  A+E A +E+   E
Sbjct: 376 QTETFDASRK-------------LGELNQRRLEEAIKLEELKLKEYEARELAEKEKQNFE 435

Query: 433 ALRREAKYVKECAEREGIYRKEAETKALQDAKEKGKHENALQGPLQQYQHFQWEDIVSAT 492
             RR+A+ ++E AERE   R+EAE K+ +D KEK K E  L  P  QYQHF WE+I++AT
Sbjct: 436 KARRDAESMRERAEREIAQRREAERKSARDTKEKEKLEGTLGSPQLQYQHFAWEEIMAAT 495

Query: 493 SSFSEDLKIGMGAHGTVYKCSLHHTTVAVKVLHSRDGHKRMQLLQELEILSRIHHPHLLL 552
           SSFSE+LKIGMGA+G VYKC+LHHTT  VKVL S +     Q  QELEILS+I HPHL+L
Sbjct: 496 SSFSEELKIGMGAYGAVYKCNLHHTTAVVKVLQSAENQLSKQFQQELEILSKIRHPHLVL 555

Query: 553 LLGACPDKNCLVYEYMENGSLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPKP 612
           LLGACP++  LVYEYMENGSLEDRL++  N+P +PW+ERFRIAWE+A+ALVFLH SKPKP
Sbjct: 556 LLGACPEQGALVYEYMENGSLEDRLFQVNNSPPLPWFERFRIAWEVAAALVFLHKSKPKP 615

Query: 613 IIHRDLKPANILLDQNLVSKIGDVGLSTVFNSDP--SMSTAFMNSGPVGTLCYIDPEYQR 672
           IIHRDLKPANILLD N VSK+GDVGLST+   DP  +  T +  + PVGTLCYIDPEYQR
Sbjct: 616 IIHRDLKPANILLDHNFVSKVGDVGLSTMVQVDPLSTKFTIYKQTSPVGTLCYIDPEYQR 675

Query: 673 TGLVSPKSDVYAFGMVILQLLTAKPAVALTHVVETAID-NCTLINVLDIEAGHWPVEETC 732
           TG +S KSD+Y+FGM++LQLLTAKPA+ALTH VE+A+D N   + +LD +AG+WP+EET 
Sbjct: 676 TGRISSKSDIYSFGMILLQLLTAKPAIALTHFVESAMDSNDEFLKILDQKAGNWPIEETR 735

Query: 733 ELARLGLRCAEMQRKDRPDLKDQVLPLLMTLKKVAHEARNLASKVPAAIPNHFICPILQD 792
           ELA L L C E++ KDRPDLKDQ+LP L  LKKVA +ARN  S V    P HFICP+L+D
Sbjct: 736 ELAALALCCTELRGKDRPDLKDQILPALENLKKVAEKARNSFSGVSTQPPTHFICPLLKD 795

Query: 793 MMNDPCVAADGYTYDRRAIEKWFQENDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSRK 819
           +MN+PCVAADGYTYDR AIE+W +E++ SPMT  PL  KNL+PNY+L +AI+EW S +
Sbjct: 796 VMNEPCVAADGYTYDRHAIEEWLKEHNTSPMTDSPLHSKNLLPNYTLYTAIMEWRSTR 835

BLAST of HG10009350 vs. ExPASy Swiss-Prot
Match: Q9FKG6 (U-box domain-containing protein 52 OS=Arabidopsis thaliana OX=3702 GN=PUB52 PE=2 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 2.8e-226
Identity = 429/848 (50.59%), Postives = 585/848 (68.99%), Query Frame = 0

Query: 6   NRNLMLLPPPSPVVAVAISGKKNSKYIIRWSLEKFLPEGITDFKLLHIIPRITSVPTPMG 65
           + +L L PPPSP VAVAI+GKK SKY++ W+LEKF+PEG TDFKLL++ P ++ +PTPMG
Sbjct: 11  SEHLSLPPPPSPSVAVAINGKKKSKYVVFWALEKFIPEGFTDFKLLYVRPPVSYIPTPMG 70

Query: 66  NSIPISQVRDDVAAAYRKEIWWQTSEKLLPYKKMFAQRKVHLDVATLEADDVANAIIEEV 125
            ++ +S++R+DV +AY++E+ W  +E L PYKKMF +RKV ++V  L++ + A AI EE+
Sbjct: 71  IAVAVSELREDVVSAYKQELDWSANEMLRPYKKMFERRKVQVEVLLLDSLEPAAAIAEEI 130

Query: 126 TKSSINKLVIGVSSQGLFSRKLNGLSSRISALAPRYCTVYAISKGKLASIRPPDMETSVS 185
             + + KLVIG+S +G FSRK++ +SS I+   PR+CTVY ISKGKLAS+RP + + S S
Sbjct: 131 AGTGVTKLVIGMSLRGFFSRKID-MSSLIATAVPRFCTVYVISKGKLASVRPSESDASGS 190

Query: 186 IKDDASEASSANSYSSYSSSSLTDASSSLITSYSHFPSPSPSLP-LQRFQALSTINQSLL 245
           I+ + S ++S ++ S        D  S++  + S     SP+L       A++ ++ S  
Sbjct: 191 IRFERSSSTSGSTDSPRLPPEYQDFLSAVSEAQSRVSPFSPALKHSMGSNAVAQMDTSSS 250

Query: 246 TTKPSPI---------------KANHSRCQSVDIEDQVDGVRSSSYVS------DCAQTL 305
            T    +               K N     S       +   S S+ S      D  +  
Sbjct: 251 GTDQEEVSTGRGMEIVHSGIEGKKNKDESFSASFPMGTEAYNSMSWTSKWRDHEDRREMR 310

Query: 306 SRTSSCK---------SLPTDNQSWVSDEAS--SSGAFNDYSSSESQADVSFELEKLRIE 365
           S +SS           ++  +N SWVS  AS  S G  + +S +++Q +++FE+EKLR E
Sbjct: 311 SSSSSNNHDLVNMDWGAVVPENYSWVSHTASHMSDGLLSVHSITDNQVNLNFEIEKLRAE 370

Query: 366 LRHARGMYAIAQRETIDASRKQEKKSHLVIVYDQLNHLNKQRSDEARKLEEINNKEVAAK 425
           L+H + MYA+AQ ET+ AS+K             L  LN++R +E+ KL E+  KE  AK
Sbjct: 371 LKHVQEMYAMAQTETVGASKK-------------LTELNQRRFEESEKLVELKEKEEVAK 430

Query: 426 EFAMEERMKREALRREAKYVKECAEREGIYRKEAETKALQDAKEKGKHENALQGPLQQYQ 485
           + A +E+ + E   +EA+ VKE   +E ++R+EAE KA +DA+EK K + +L  P  QYQ
Sbjct: 431 DTASKEKQRYEEAMKEAEKVKELMMKEALHRREAEFKAERDAREKDKLQASLVSPGVQYQ 490

Query: 486 HFQWEDIVSATSSFSEDLKIGMGAHGTVYKCSLHHTTVAVKVLHSRDGHKRMQLLQELEI 545
           H+ WE+I +ATS F+E+LKIG+GA+G+VYKC+LHHTT AVKVLH+ +     Q  QELEI
Sbjct: 491 HYTWEEIAAATSDFAENLKIGIGAYGSVYKCNLHHTTGAVKVLHAGETQLSKQFDQELEI 550

Query: 546 LSRIHHPHLLLLLGACPDKNCLVYEYMENGSLEDRLYRRGNTPAIPWYERFRIAWEIASA 605
           LS+I HPHL+LLLGACP++ CLVYEYM+NGSL+DRL    +TP IPW+ERFRIA E+ASA
Sbjct: 551 LSKIRHPHLVLLLGACPERGCLVYEYMDNGSLDDRLMLVNDTPPIPWFERFRIALEVASA 610

Query: 606 LVFLHSSKPKPIIHRDLKPANILLDQNLVSKIGDVGLSTVFNSDP-SMSTAFMNSGPVGT 665
           LVFLH SKP+PIIHRDLKP NILLD N VSK+GDVGLST+ N D  S  T F  + PVGT
Sbjct: 611 LVFLHKSKPRPIIHRDLKPGNILLDHNFVSKLGDVGLSTMVNQDDVSSRTIFKQTSPVGT 670

Query: 666 LCYIDPEYQRTGLVSPKSDVYAFGMVILQLLTAKPAVALTHVVETAI-DNCTLINVLDIE 725
           LCYIDPEYQRTG++SPKSDVY+ G+VILQL+TAKPA+A+TH+VE AI D+   + +LD +
Sbjct: 671 LCYIDPEYQRTGIISPKSDVYSLGVVILQLITAKPAIAITHMVEEAIGDDAEFMAILDKK 730

Query: 726 AGHWPVEETCELARLGLRCAEMQRKDRPDLKDQVLPLLMTLKKVAHEARNLASKVPAAIP 785
           AG WP+ +T ELA LGL C EM+R+DRPDLKDQ++P L  L+KVA +A+NL S+ P+  P
Sbjct: 731 AGSWPISDTRELAALGLCCTEMRRRDRPDLKDQIIPALERLRKVADKAQNLLSRTPSGPP 790

Query: 786 NHFICPILQDMMNDPCVAADGYTYDRRAIEKWFQENDNSPMTKLPLPDKNLIPNYSLLSA 819
           +HFICP+L+ +MN+PCVAADGYTYDR AIE+W ++ D SP+T LPLP+KNLI NY+L SA
Sbjct: 791 SHFICPLLKGVMNEPCVAADGYTYDREAIEEWLRQKDTSPVTNLPLPNKNLIANYTLYSA 844

BLAST of HG10009350 vs. ExPASy Swiss-Prot
Match: Q9FKG5 (U-box domain-containing protein 51 OS=Arabidopsis thaliana OX=3702 GN=PUB51 PE=2 SV=2)

HSP 1 Score: 689.1 bits (1777), Expect = 6.0e-197
Identity = 405/827 (48.97%), Postives = 547/827 (66.14%), Query Frame = 0

Query: 18  VVAVAISGKKN-SKYIIRWSLEKFLPEGITDFKLLHIIPRITSVPTPMGNSIPISQVRDD 77
           +VAVAI G  + +K ++RW+L++F  +    FKLLH+ PR         +S  +S  R D
Sbjct: 7   IVAVAIKGNNSKTKGVVRWALQEFASQEHVVFKLLHVQPR---------DSNSVSTTRKD 66

Query: 78  V-AAAYRKEIWWQTSEKLLPYKKMFAQRKVHLDVATLEADDVANAIIEEVTKSSINKLVI 137
           +  + Y+K++  +T E LLP + MF  R+V LD+  LE+DD+A+AI + V    I++LVI
Sbjct: 67  LTTSVYKKDVDRKTREMLLPSRDMFVHREVQLDIMVLESDDIADAISKAVQDHGISELVI 126

Query: 138 GVSSQGLFSRKL--NGLSSRISALAPRYCTVYAISKGKLASIRPPDMETSVSIKDDASEA 197
           G SS  +FS KL  + LSSRI+   PR+C+V+ ISKGKL ++R  DM+T  SI DD SE 
Sbjct: 127 GASSSIIFSWKLKRSNLSSRIADATPRFCSVHVISKGKLLNVRKSDMDTETSIADDRSE- 186

Query: 198 SSANSYSSYSSSSLTDASSSLITSYSHFPSPSPSLPLQRFQALSTINQSLLTT----KPS 257
                 S +SS    D+ S  ++S S     S  L  QR QAL+T+NQ + T        
Sbjct: 187 ------SRFSS----DSHSGTVSSTSSHQFSSTPLLFQRIQALTTVNQKVGTNIGKQNNE 246

Query: 258 PIKANHSRCQSVDIEDQVDGVRSSSYVSDCAQTLSRTSSCKSLPTDNQSWVS---DEASS 317
           P   +H+R  S+D+++       S  ++      + +S      +D  SW S   +EASS
Sbjct: 247 PHHHHHNRAGSLDVDE-------SKLLNQKGFYRTSSSGIGYGGSDISSWRSSQMEEASS 306

Query: 318 SGAFNDYSSSESQADVSFELEKLRIELRHARGMYAIAQRETIDASRKQEKKSHLVIVYDQ 377
           S  ++D +SS SQ    FELEKL+IELRH +GMYA+AQ E IDAS+K             
Sbjct: 307 SSTYSDPTSSSSQIHKDFELEKLKIELRHIKGMYAVAQSEVIDASKK------------- 366

Query: 378 LNHLNKQRSDEARKLEEINNKEVAAKEFAMEERMKREALRREAKYVKECAEREGIYRKEA 437
           +  LN++RS+EA +L+ +  +E  A E    ER ++E    EA+ V+EC ERE   R EA
Sbjct: 367 MQDLNQRRSEEATRLKNLTIREEEADEVVEMERERQEDAENEAELVRECIERETEERLEA 426

Query: 438 ETKALQDAKEKGKHENALQ-GPL--QQYQHFQWEDIVSATSSFSEDLKIGMGAHGTVYKC 497
           E +A +  KEK + E+AL+ GPL  QQY  F+WE+IV ATSSFS++LKIG+G +G+VY+C
Sbjct: 427 EARAEEVRKEKQRLEDALEGGPLQRQQYMKFEWEEIVEATSSFSDELKIGVGGYGSVYRC 486

Query: 498 SLHHTTVAVKVLHSRDGHKRMQLLQELEILSRIHHPHLLLLLGACPDKNCLVYEYMENGS 557
           +LHHTTVAVKVLHS       Q  QELEILS+I HPHLLLLLGACP++  LVYEYM NGS
Sbjct: 487 NLHHTTVAVKVLHSDKSSLTKQFHQELEILSKIRHPHLLLLLGACPERGSLVYEYMHNGS 546

Query: 558 LEDRLYRR------GNTPAIPWYERFRIAWEIASALVFLHSSKPKPIIHRDLKPANILLD 617
           LE+RL +R         P + W+ERFRIAWEIASAL FLH+++P+PI+HRDLKPANILLD
Sbjct: 547 LEERLMKRRPNVDTPQPPPLRWFERFRIAWEIASALYFLHTNEPRPIVHRDLKPANILLD 606

Query: 618 QNLVSKIGDVGLSTVFNSDPS-MSTAFMNSGPVGTLCYIDPEYQRTGLVSPKSDVYAFGM 677
           +N VSKIGDVGLS + N DPS  ST F  +GPVGT  YIDPEYQRTG+V+P+SD+YAFG+
Sbjct: 607 RNNVSKIGDVGLSKMVNLDPSHASTVFNETGPVGTFFYIDPEYQRTGVVTPESDIYAFGI 666

Query: 678 VILQLLTAKPAVALTHVVETAIDNCT--LINVLDIEAGHWPVEETCELARLGLRCAEMQR 737
           ++LQL+TA+ A+ L H +E A+ + T     +LD  AG WPV+E  E+  +GLRCAEM++
Sbjct: 667 ILLQLVTARSAMGLAHSIEKALRDQTGKFTEILDKTAGDWPVKEAKEMVMIGLRCAEMRK 726

Query: 738 KDRPDLKDQVLPLLMTLKKVAHEARNLAS----KVPAAIPNHFICPILQDMMNDPCVAAD 797
           +DRPDL  ++LP+L  LK+VA  ARN+ +          P HF CPI +D+M +PCVA+D
Sbjct: 727 RDRPDLGKEILPVLERLKEVASIARNMFADNLIDHHHNAPTHFYCPITKDVMENPCVASD 786

Query: 798 GYTYDRRAIEKWFQENDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSR 818
           GYTY++RAI++W Q+N  SPMT LP P  +L+PN+SLLSAI EW S+
Sbjct: 787 GYTYEKRAIKEWLQKNHKSPMTDLPFPSDSLLPNHSLLSAIKEWRSQ 793

BLAST of HG10009350 vs. ExPASy Swiss-Prot
Match: Q9LU47 (Putative U-box domain-containing protein 53 OS=Arabidopsis thaliana OX=3702 GN=PUB53 PE=3 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 1.5e-184
Identity = 393/836 (47.01%), Postives = 528/836 (63.16%), Query Frame = 0

Query: 11  LLPPPSPV-VAVAISGKKNSKYIIRWSLEKFLPEGITDFKLLHIIPRITSVPTPMGNSIP 70
           L  P  P+ VA+AISG   SK +I+W+L KF  +    FKL+HI P+IT++PT  GN + 
Sbjct: 25  LAAPSEPMTVALAISGSIKSKNVIKWALNKFGSDKNVTFKLIHIHPKITTLPTASGNIVS 84

Query: 71  ISQVRDDVAAAYRKEIWWQTSEKLL-PYKKMFAQRKVHLD--------------VATLEA 130
           IS+  ++VAAAYR+++  +T E LL P+KKM  ++K+ +D              +  LE+
Sbjct: 85  ISEELEEVAAAYRQKVMQETKETLLKPFKKMCERKKLKIDETRFESSLTKVAVELQVLES 144

Query: 131 DDVANAIIEEVTKSSINKLVIGVSSQGLFSRKLNGLSSRISALAPRYCTVYAISKGKLAS 190
           + VA AI +EV +  I+ L+IG SSQ   SR  + +++ ISA     CTVY +S G +  
Sbjct: 145 NSVAVAITKEVNQHLISNLIIGRSSQAASSRNYD-ITASISASVSNLCTVYVVSNGGVHI 204

Query: 191 IRPPDMETSVSIKDDASEASSANSYSSYSSSSLTDASSSLITSYSHFPSPSPSLPLQRFQ 250
           +     +T  +     S     +S  S  S + +D  S+ + S  H      +L  +R Q
Sbjct: 205 LAKDTSDTERNDTSIESGFERTSSSCSSGSGANSDVMSNALKSNPH------TLSNKRMQ 264

Query: 251 ALSTINQSL-LTTKPSPIKANHSRCQSVDIEDQVDGVRSSSYVSDCAQTLSRTSSCKSLP 310
            L TI + + +  + S  +++ ++ +S D  ++    RSS    + ++++S     +   
Sbjct: 265 NLPTIVRGVSVPMETSSTESDETKKRSSDAAEEA-SKRSS---PETSRSVSWNPQFRDF- 324

Query: 311 TDNQSWVSDEASSSGAFNDYSSS-----ESQADVSFELEKLRIELRHARGMYAIAQRETI 370
            D +       SS+  + +  +          D   E+ KLR ELRHA  MYA+AQ ET+
Sbjct: 325 -DERKDAMSSMSSNFEYGNVVTPLGHYFTDNQDTLNEISKLRAELRHAHEMYAVAQVETL 384

Query: 371 DASRKQEKKSHLVIVYDQLNHLNKQRSDEARKLEEINNKEVAAKEFAMEERMKREALRRE 430
           DASRK             LN L         K EE+   E   K  A +E  K E  RRE
Sbjct: 385 DASRK-------------LNEL---------KFEELTLLEHETKGIAKKETEKFEQKRRE 444

Query: 431 AKYVKECAEREGIYRKEAETKALQDAKEKGK-HENALQGPLQQYQHFQWEDIVSATSSFS 490
                   ERE   R+EAE KA  +AKEK K  E++L  P  QYQ F WE+I++ATSSFS
Sbjct: 445 --------EREAAQRREAEMKATHEAKEKEKLEESSLVAPKLQYQEFTWEEIINATSSFS 504

Query: 491 EDLKIGMGAHGTVYKCSLHHTTVAVKVLHSRDGHKRMQLLQELEILSRIHHPHLLLLLGA 550
           EDLKIGMGA+G VYKC+LHHT  AVKVLHS +     Q  QELEILS+I HPHL+LLLGA
Sbjct: 505 EDLKIGMGAYGDVYKCNLHHTIAAVKVLHSAESSLSKQFDQELEILSKIRHPHLVLLLGA 564

Query: 551 CPDKNCLVYEYMENGSLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPKPIIHR 610
           CPD   LVYEYMENGSLEDRL++  ++  IPW+ R RIAWE+ASALVFLH SKP PIIHR
Sbjct: 565 CPDHGALVYEYMENGSLEDRLFQVNDSQPIPWFVRLRIAWEVASALVFLHKSKPTPIIHR 624

Query: 611 DLKPANILLDQNLVSKIGDVGLSTVFNSDPSMSTAF---MNSGPVGTLCYIDPEYQRTGL 670
           DLKPANILL+ N VSK+GDVGLST+  +   +ST F     + PVGTLCYIDPEYQRTG 
Sbjct: 625 DLKPANILLNHNFVSKVGDVGLSTMIQAADPLSTKFTMYKQTSPVGTLCYIDPEYQRTGR 684

Query: 671 VSPKSDVYAFGMVILQLLTAKPAVALTHVVETAIDNCT---LINVLDIEAGHWPVEETCE 730
           +SPKSDVYAFGM+ILQLLT + A+ALT+ VETA++N     LI +LD +AG+WP+EET +
Sbjct: 685 ISPKSDVYAFGMIILQLLTGQQAMALTYTVETAMENNNDDELIQILDEKAGNWPIEETRQ 744

Query: 731 LARLGLRCAEMQRKDRPDLKDQVLPLLMTLKKVAHEARNLASKVPAAIPNHFICPILQDM 790
           LA L L+C E++ KDRPDL+DQ+LP+L +LKKVA +ARN  S  P+  P+HF CP+L+D+
Sbjct: 745 LAALALQCTELRSKDRPDLEDQILPVLESLKKVADKARNSLSAAPSQPPSHFFCPLLKDV 804

Query: 791 MNDPCVAADGYTYDRRAIEKWFQENDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSR 818
           M +PC+AADGYTYDRRAIE+W + +  SP+T  PL + NL+PN++L +AIVEW +R
Sbjct: 805 MKEPCIAADGYTYDRRAIEEWMENHRTSPVTNSPLQNVNLLPNHTLYAAIVEWRNR 817

BLAST of HG10009350 vs. ExPASy Swiss-Prot
Match: Q8S8S7 (U-box domain-containing protein 34 OS=Arabidopsis thaliana OX=3702 GN=PUB34 PE=3 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 1.9e-113
Identity = 288/841 (34.24%), Postives = 440/841 (52.32%), Query Frame = 0

Query: 19  VAVAIS-------GKKNSKYIIRWSLEKFLPEGITDFKLLHIIPRITSVPTP-------- 78
           VAVA+        G   S+  +RW+++  LP+    F ++H+IP ITS+PTP        
Sbjct: 24  VAVAVKGLIGDKLGGAGSRRAVRWAVDNLLPKA-DKFVMIHVIPTITSIPTPNILILMFT 83

Query: 79  ------MGNSIPISQVRDDVAAAYRKEIWWQTSEKLLPYKKMFAQRKVHLDVATLEADDV 138
                  G+ +P+ +V + V   Y +++  +     +P+ KM    +             
Sbjct: 84  RMWVVTAGDRLPVEEVEESVVEMYVRDVKKEYETVFVPFLKMCKSTR------------- 143

Query: 139 ANAIIEEVTKSSINKLVIGVSSQGLF-SRKLNGLSSRISAL--APRYCTVYAISKGKLAS 198
                               S++  F SR+  G    ++ L  AP  C VY + K ++ +
Sbjct: 144 --------------------STKRYFRSRRTKGTGVPLTVLRYAPETCEVYIVCKDRITT 203

Query: 199 IRPPDMETSVSIKDDASEASSANSYSSYSSSSLTDASSSLITSYS-HFPSP--------- 258
                M+  ++ +   S  ++A ++       L D ++S  T  S   P P         
Sbjct: 204 ---KSMDPLINREPCTSPHAAATAH-----DFLRDWAASFHTLRSPTLPDPRQSTEAGTR 263

Query: 259 -SPSLPLQRFQALS-TINQSLLTTKPSPIKANHSRCQSVDIEDQVDGVRSSSYVSDCAQT 318
            S S    RF+ALS T N+      P   KA+ +    +    +   +   +Y SD  +T
Sbjct: 264 RSASARELRFEALSLTCNK---PKTPQSSKASSATTPEIFRRRRGSDIPQLNY-SDFDKT 323

Query: 319 LSRTSSCKSLPTDNQSWVSDEASSSGAFNDYSSSESQADVSFELEKLRIELRHARGMYAI 378
                 C    ++ ++ VS+   S  +  + S    + ++  E+E+L+ EL+     Y  
Sbjct: 324 ------CTKPQSNVENIVSEHRDSDRSPPETSRKSKKVEIEEEVERLKNELQSTVFKYKQ 383

Query: 379 AQRETIDASRKQEKKSHLVIVYDQLNHLNKQRSDEARKLEEINNKEVAAKEFAMEERMKR 438
           A  E      K             +  L+ +  +E++++     KE   +  A  E+ + 
Sbjct: 384 ACEELFSTQNK-------------VKMLSTEYLNESKRVNNAVEKEELQRNTAALEKERY 443

Query: 439 EALRREAKYVKECAEREGIYRKEAETKALQDAKEKGKHENALQGPLQQYQHFQWEDIVSA 498
               +E +  K    RE   R+ AE  AL+   EK K  + L G   +Y+ +  E+IV+A
Sbjct: 444 MKAVKEVETAKALLAREFCQRQIAEVNALRTYLEKKKVIDQLLGTDHRYRKYTIEEIVTA 503

Query: 499 TSSFSEDLKIGMGAHGTVYKCSLHHTTVAVKVLHSRDGHKRMQLLQELEILSRIHHPHLL 558
           T  FS +  IG G +G VY+CSL  T  AVKV+      K+ + L+E+E+LS++ HPH++
Sbjct: 504 TEGFSPEKVIGEGGYGKVYQCSLDSTPAAVKVVRLDTPEKKQEFLKEVEVLSQLRHPHVV 563

Query: 559 LLLGACPDKNCLVYEYMENGSLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPK 618
           LLLGACP+  CLVYEY+ENGSLE+ ++ R N P +PW+ RFR+ +E+A  L FLHSSKP+
Sbjct: 564 LLLGACPENGCLVYEYLENGSLEEYIFHRKNKPPLPWFIRFRVIFEVACGLAFLHSSKPE 623

Query: 619 PIIHRDLKPANILLDQNLVSKIGDVGLS-TVFNSDPSMSTAFMNSGPVGTLCYIDPEYQR 678
           PI+HRDLKP NILL++N VSKI DVGL+  V +  P   T + NS   GTL YIDPEY R
Sbjct: 624 PIVHRDLKPGNILLNRNYVSKIADVGLAKLVTDVAPDNVTMYRNSVLAGTLHYIDPEYHR 683

Query: 679 TGLVSPKSDVYAFGMVILQLLTAKPAVALTHVVETAIDNCTLINVLDIEAGHWPVEETCE 738
           TG + PKSD+YAFG++ILQLLTA+    +   VE A+   TL  +LD     WP+ ET E
Sbjct: 684 TGTIRPKSDLYAFGIIILQLLTARNPSGIVPAVENAVKKGTLTEMLDKSVTDWPLAETEE 743

Query: 739 LARLGLRCAEMQRKDRPDLKDQVLPLLMTL-----KKVAHEARNLASKVPAAIPNHFICP 798
           LAR+GL+CAE + +DRPDLK +V+P+L  L      KV  E  NL +      P+H+ CP
Sbjct: 744 LARIGLKCAEFRCRDRPDLKSEVIPVLKRLVETANSKVKKEGSNLRA------PSHYFCP 793

Query: 799 ILQDMMNDPCVAADGYTYDRRAIEKWFQENDNSPMTKLPLPDKNLIPNYSLLSAIVEWNS 818
           IL+++M +P +AADG+TY+R+AI  W ++++ SP+T+  L    L PN++L SAI +W S
Sbjct: 804 ILREIMEEPEIAADGFTYERKAILAWLEKHNISPVTRQKLDHFKLTPNHTLRSAIRDWKS 793

BLAST of HG10009350 vs. ExPASy TrEMBL
Match: A0A1S3ATK7 (E3 ubiquitin ligase OS=Cucumis melo OX=3656 GN=LOC103482570 PE=4 SV=1)

HSP 1 Score: 1419.8 bits (3674), Expect = 0.0e+00
Identity = 734/817 (89.84%), Postives = 765/817 (93.64%), Query Frame = 0

Query: 3   DEANRNLMLLPPPSPVVAVAISGKKNSKYIIRWSLEKFLPEGITDFKLLHIIPRITSVPT 62
           D+A +N MLLP  SPVVAVAISGKKNSKYIIRWSLEKFLPEGI DFKLLH  PRITSVPT
Sbjct: 6   DQAKKNHMLLPSSSPVVAVAISGKKNSKYIIRWSLEKFLPEGIIDFKLLHFFPRITSVPT 65

Query: 63  PMGNSIPISQVRDDVAAAYRKEIWWQTSEKLLPYKKMFAQRKVHLDVATLEADDVANAII 122
           PMGN+IP+SQVR+DVA AYRKEIWWQTSEKLLP+KKMFAQRKVH+DV TLEADDV +AII
Sbjct: 66  PMGNAIPVSQVREDVAVAYRKEIWWQTSEKLLPFKKMFAQRKVHVDVVTLEADDVVDAII 125

Query: 123 EEVTKSSINKLVIGVSSQGLFSRKLNGLSSRISALAPRYCTVYAISKGKLASIRPPDMET 182
           EEVTK SINKLVIGVSSQGLFSRKL+GLSSRISALAPRYCTVYAISKGKLASIRPPDM+T
Sbjct: 126 EEVTKCSINKLVIGVSSQGLFSRKLSGLSSRISALAPRYCTVYAISKGKLASIRPPDMDT 185

Query: 183 SVSIKDDASEASSANSYSSYSSSSLTDASSSLITSYSHFPSPSPSLPLQRFQALSTINQS 242
            VSI+DDASE SSA+SYSSY+SSSLTD SSSL TSYSHFPSPSPSLPLQRFQALSTINQ 
Sbjct: 186 DVSIRDDASEESSASSYSSYTSSSLTDGSSSLTTSYSHFPSPSPSLPLQRFQALSTINQP 245

Query: 243 LLTTKPSPIKANHSRCQSVDIEDQVDGVRSSSYVSDCAQTLSRTSSCKSLPTDNQSWVSD 302
           LLT KPS IKA+HSRCQS+DIE  VDGVRSSS+VSDC QTLSR SS KS PT+NQSW+SD
Sbjct: 246 LLTKKPSLIKADHSRCQSIDIE-VVDGVRSSSHVSDCTQTLSRASSSKSSPTENQSWISD 305

Query: 303 EASSSGAFNDYSSSESQADVSFELEKLRIELRHARGMYAIAQRETIDASRKQEKKSHLVI 362
           EASSSGAFNDYSS ESQADVSFELEKLRIELRHARGMYAIAQRETIDASR          
Sbjct: 306 EASSSGAFNDYSSCESQADVSFELEKLRIELRHARGMYAIAQRETIDASR---------- 365

Query: 363 VYDQLNHLNKQRSDEARKLEEINNKEVAAKEFAMEERMKREALRREAKYVKECAEREGIY 422
              +LNHLNKQRS+EARKLEEINNK VAAKEFA EER+K EALRREAKYVKE AEREGIY
Sbjct: 366 ---ELNHLNKQRSEEARKLEEINNKVVAAKEFAREERVKHEALRREAKYVKERAEREGIY 425

Query: 423 RKEAETKALQDAKEKGKHENALQGPLQQYQHFQWEDIVSATSSFSEDLKIGMGAHGTVYK 482
           RKEAETKALQDAKEKGKHENALQGPLQQYQHFQWEDIVSAT SFSEDLKIGMGAHGTVYK
Sbjct: 426 RKEAETKALQDAKEKGKHENALQGPLQQYQHFQWEDIVSATLSFSEDLKIGMGAHGTVYK 485

Query: 483 CSLHHTTVAVKVLHSRDGHKRMQLLQELEILSRIHHPHLLLLLGACPDKNCLVYEYMENG 542
           CSLHHTTVAVKVLHSRD HK+MQLLQELE+LSRIHHPHLLLLLGACPDKNCLVYEYMENG
Sbjct: 486 CSLHHTTVAVKVLHSRDSHKQMQLLQELEVLSRIHHPHLLLLLGACPDKNCLVYEYMENG 545

Query: 543 SLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDQNLVS 602
           SLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPK IIHRDLKPANILLDQNLVS
Sbjct: 546 SLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPKSIIHRDLKPANILLDQNLVS 605

Query: 603 KIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQRTGLVSPKSDVYAFGMVILQLL 662
           KIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQRTGL+SPKSDVYAFGMVILQLL
Sbjct: 606 KIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQLL 665

Query: 663 TAKPAVALTHVVETAIDNCTLINVLDIEAGHWPVEETCELARLGLRCAEMQRKDRPDLKD 722
           TAKPAVALTHVVETAIDN  LI VLDIEAGHWP+EET ELARLGLRCAEMQRKDRPDLKD
Sbjct: 666 TAKPAVALTHVVETAIDNSNLIKVLDIEAGHWPLEETYELARLGLRCAEMQRKDRPDLKD 725

Query: 723 QVLPLLMTLKKVAHEARNLASKVPAAIPNHFICPILQDMMNDPCVAADGYTYDRRAIEKW 782
           QVLPLLMTLKKVA EARN ASKVPAAIPNHFICPILQD+MNDPCVAADGYTYDR+AIEKW
Sbjct: 726 QVLPLLMTLKKVADEARNFASKVPAAIPNHFICPILQDVMNDPCVAADGYTYDRQAIEKW 785

Query: 783 FQENDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSRKN 820
            Q+NDNSPMTKLPLPDKNLIPNYSLLSAIVEWNS+++
Sbjct: 786 LQKNDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSKRS 808

BLAST of HG10009350 vs. ExPASy TrEMBL
Match: A0A0A0KL01 (E3 ubiquitin ligase OS=Cucumis sativus OX=3659 GN=Csa_5G167050 PE=4 SV=1)

HSP 1 Score: 1416.4 bits (3665), Expect = 0.0e+00
Identity = 734/817 (89.84%), Postives = 766/817 (93.76%), Query Frame = 0

Query: 3   DEANRNLMLLPPPSPVVAVAISGKKNSKYIIRWSLEKFLPEGITDFKLLHIIPRITSVPT 62
           D+A RN MLL P S VVAVAISGKKNSKYIIRWSLEKFLPEGI DF+LLH IPRITSVPT
Sbjct: 55  DQAKRNHMLL-PSSSVVAVAISGKKNSKYIIRWSLEKFLPEGIIDFRLLHFIPRITSVPT 114

Query: 63  PMGNSIPISQVRDDVAAAYRKEIWWQTSEKLLPYKKMFAQRKVHLDVATLEADDVANAII 122
           PMGN+IPISQVR+DVAAAYRKEIWW TSEKLLP+KKMFAQRKVHLDV TLEADDVA AII
Sbjct: 115 PMGNAIPISQVREDVAAAYRKEIWWHTSEKLLPFKKMFAQRKVHLDVVTLEADDVAGAII 174

Query: 123 EEVTKSSINKLVIGVSSQGLFSRKLNGLSSRISALAPRYCTVYAISKGKLASIRPPDMET 182
           EEVTK SINKLVIGVSSQGLFSRKL+GLSSRISALAPRYCTVYAISKGKLASIRPPDM+T
Sbjct: 175 EEVTKCSINKLVIGVSSQGLFSRKLSGLSSRISALAPRYCTVYAISKGKLASIRPPDMDT 234

Query: 183 SVSIKDDASEASSANSYSSYSSSSLTDASSSLITSYSHFPSPSPSLPLQRFQALSTINQS 242
           +VSI+DDASE SSA+SYSSY+SSSLTD SSSL +SYSHFPSPSPSLPLQRFQALSTINQ 
Sbjct: 235 NVSIRDDASEESSASSYSSYTSSSLTDGSSSLTSSYSHFPSPSPSLPLQRFQALSTINQP 294

Query: 243 LLTTKPSPIKANHSRCQSVDIEDQVDGVRSSSYVSDCAQTLSRTSSCKSLPTDNQSWVSD 302
           LLT KPSPIKA+HSRCQSVDIE+QVDGV SSSYVSDC QTLSR SS KS P +N+SW SD
Sbjct: 295 LLTKKPSPIKADHSRCQSVDIENQVDGVHSSSYVSDCIQTLSRASSSKSSPAENKSWNSD 354

Query: 303 EASSSGAFNDYSSSESQADVSFELEKLRIELRHARGMYAIAQRETIDASRKQEKKSHLVI 362
           EASSSG FNDYSS ESQADVSFELEKLRIELRHARGM+AIAQRETIDASR          
Sbjct: 355 EASSSGMFNDYSSCESQADVSFELEKLRIELRHARGMFAIAQRETIDASR---------- 414

Query: 363 VYDQLNHLNKQRSDEARKLEEINNKEVAAKEFAMEERMKREALRREAKYVKECAEREGIY 422
              +LNHLN QRS+EARKLEEINNK VAAKEFA EER+K EALRREAKYVKE AEREGIY
Sbjct: 415 ---ELNHLNNQRSEEARKLEEINNKAVAAKEFAREERVKHEALRREAKYVKERAEREGIY 474

Query: 423 RKEAETKALQDAKEKGKHENALQGPLQQYQHFQWEDIVSATSSFSEDLKIGMGAHGTVYK 482
           RKEAE KALQ+AKEKGKHENALQGPLQQYQHFQWEDIVSATSSFSEDLKIGMGAHGTVYK
Sbjct: 475 RKEAEMKALQNAKEKGKHENALQGPLQQYQHFQWEDIVSATSSFSEDLKIGMGAHGTVYK 534

Query: 483 CSLHHTTVAVKVLHSRDGHKRMQLLQELEILSRIHHPHLLLLLGACPDKNCLVYEYMENG 542
           CSLHHTTVAVKVLHSRD HK+MQLLQELE+LSRIHHPHLLLLLGACPDKNCLVYEYMENG
Sbjct: 535 CSLHHTTVAVKVLHSRDSHKQMQLLQELEVLSRIHHPHLLLLLGACPDKNCLVYEYMENG 594

Query: 543 SLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDQNLVS 602
           SLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPK IIHRDLKPANILLDQNLVS
Sbjct: 595 SLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPKSIIHRDLKPANILLDQNLVS 654

Query: 603 KIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQRTGLVSPKSDVYAFGMVILQLL 662
           KIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQRTGL+SPKSDVYAFGMVILQLL
Sbjct: 655 KIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQLL 714

Query: 663 TAKPAVALTHVVETAIDNCTLINVLDIEAGHWPVEETCELARLGLRCAEMQRKDRPDLKD 722
           TAKPAVALTHVVETAIDN  LINVLDIEAGHWP+EET ELARLGLRCAEMQRKDRPDLKD
Sbjct: 715 TAKPAVALTHVVETAIDNSNLINVLDIEAGHWPLEETYELARLGLRCAEMQRKDRPDLKD 774

Query: 723 QVLPLLMTLKKVAHEARNLASKVPAAIPNHFICPILQDMMNDPCVAADGYTYDRRAIEKW 782
           QVLPLLMTLKKVA +ARNLASKVPAAIPNHFICPILQD+MNDPCVAADGYTYDR+AIEKW
Sbjct: 775 QVLPLLMTLKKVADKARNLASKVPAAIPNHFICPILQDVMNDPCVAADGYTYDRQAIEKW 834

Query: 783 FQENDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSRKN 820
            Q+NDNSPMTKLPLPDKNLIPNYSLLSAIVEWNS+++
Sbjct: 835 LQKNDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSKRS 857

BLAST of HG10009350 vs. ExPASy TrEMBL
Match: A0A1S4DSC4 (E3 ubiquitin ligase OS=Cucumis melo OX=3656 GN=LOC103482570 PE=4 SV=1)

HSP 1 Score: 1409.4 bits (3647), Expect = 0.0e+00
Identity = 733/829 (88.42%), Postives = 765/829 (92.28%), Query Frame = 0

Query: 3   DEANRNLMLLPPPSPVVAVAISGKKNSKYIIRWSLEKFLPEGITDFKLLHIIPRITSVPT 62
           D+A +N MLLP  SPVVAVAISGKKNSKYIIRWSLEKFLPEGI DFKLLH  PRITSVPT
Sbjct: 6   DQAKKNHMLLPSSSPVVAVAISGKKNSKYIIRWSLEKFLPEGIIDFKLLHFFPRITSVPT 65

Query: 63  P------------MGNSIPISQVRDDVAAAYRKEIWWQTSEKLLPYKKMFAQRKVHLDVA 122
           P            +GN+IP+SQVR+DVA AYRKEIWWQTSEKLLP+KKMFAQRKVH+DV 
Sbjct: 66  PNCIFKLYTNVDAVGNAIPVSQVREDVAVAYRKEIWWQTSEKLLPFKKMFAQRKVHVDVV 125

Query: 123 TLEADDVANAIIEEVTKSSINKLVIGVSSQGLFSRKLNGLSSRISALAPRYCTVYAISKG 182
           TLEADDV +AIIEEVTK SINKLVIGVSSQGLFSRKL+GLSSRISALAPRYCTVYAISKG
Sbjct: 126 TLEADDVVDAIIEEVTKCSINKLVIGVSSQGLFSRKLSGLSSRISALAPRYCTVYAISKG 185

Query: 183 KLASIRPPDMETSVSIKDDASEASSANSYSSYSSSSLTDASSSLITSYSHFPSPSPSLPL 242
           KLASIRPPDM+T VSI+DDASE SSA+SYSSY+SSSLTD SSSL TSYSHFPSPSPSLPL
Sbjct: 186 KLASIRPPDMDTDVSIRDDASEESSASSYSSYTSSSLTDGSSSLTTSYSHFPSPSPSLPL 245

Query: 243 QRFQALSTINQSLLTTKPSPIKANHSRCQSVDIEDQVDGVRSSSYVSDCAQTLSRTSSCK 302
           QRFQALSTINQ LLT KPS IKA+HSRCQS+DIE  VDGVRSSS+VSDC QTLSR SS K
Sbjct: 246 QRFQALSTINQPLLTKKPSLIKADHSRCQSIDIE-VVDGVRSSSHVSDCTQTLSRASSSK 305

Query: 303 SLPTDNQSWVSDEASSSGAFNDYSSSESQADVSFELEKLRIELRHARGMYAIAQRETIDA 362
           S PT+NQSW+SDEASSSGAFNDYSS ESQADVSFELEKLRIELRHARGMYAIAQRETIDA
Sbjct: 306 SSPTENQSWISDEASSSGAFNDYSSCESQADVSFELEKLRIELRHARGMYAIAQRETIDA 365

Query: 363 SRKQEKKSHLVIVYDQLNHLNKQRSDEARKLEEINNKEVAAKEFAMEERMKREALRREAK 422
           SR             +LNHLNKQRS+EARKLEEINNK VAAKEFA EER+K EALRREAK
Sbjct: 366 SR-------------ELNHLNKQRSEEARKLEEINNKVVAAKEFAREERVKHEALRREAK 425

Query: 423 YVKECAEREGIYRKEAETKALQDAKEKGKHENALQGPLQQYQHFQWEDIVSATSSFSEDL 482
           YVKE AEREGIYRKEAETKALQDAKEKGKHENALQGPLQQYQHFQWEDIVSAT SFSEDL
Sbjct: 426 YVKERAEREGIYRKEAETKALQDAKEKGKHENALQGPLQQYQHFQWEDIVSATLSFSEDL 485

Query: 483 KIGMGAHGTVYKCSLHHTTVAVKVLHSRDGHKRMQLLQELEILSRIHHPHLLLLLGACPD 542
           KIGMGAHGTVYKCSLHHTTVAVKVLHSRD HK+MQLLQELE+LSRIHHPHLLLLLGACPD
Sbjct: 486 KIGMGAHGTVYKCSLHHTTVAVKVLHSRDSHKQMQLLQELEVLSRIHHPHLLLLLGACPD 545

Query: 543 KNCLVYEYMENGSLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPKPIIHRDLK 602
           KNCLVYEYMENGSLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPK IIHRDLK
Sbjct: 546 KNCLVYEYMENGSLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPKSIIHRDLK 605

Query: 603 PANILLDQNLVSKIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQRTGLVSPKSD 662
           PANILLDQNLVSKIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQRTGL+SPKSD
Sbjct: 606 PANILLDQNLVSKIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQRTGLISPKSD 665

Query: 663 VYAFGMVILQLLTAKPAVALTHVVETAIDNCTLINVLDIEAGHWPVEETCELARLGLRCA 722
           VYAFGMVILQLLTAKPAVALTHVVETAIDN  LI VLDIEAGHWP+EET ELARLGLRCA
Sbjct: 666 VYAFGMVILQLLTAKPAVALTHVVETAIDNSNLIKVLDIEAGHWPLEETYELARLGLRCA 725

Query: 723 EMQRKDRPDLKDQVLPLLMTLKKVAHEARNLASKVPAAIPNHFICPILQDMMNDPCVAAD 782
           EMQRKDRPDLKDQVLPLLMTLKKVA EARN ASKVPAAIPNHFICPILQD+MNDPCVAAD
Sbjct: 726 EMQRKDRPDLKDQVLPLLMTLKKVADEARNFASKVPAAIPNHFICPILQDVMNDPCVAAD 785

Query: 783 GYTYDRRAIEKWFQENDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSRKN 820
           GYTYDR+AIEKW Q+NDNSPMTKLPLPDKNLIPNYSLLSAIVEWNS+++
Sbjct: 786 GYTYDRQAIEKWLQKNDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSKRS 820

BLAST of HG10009350 vs. ExPASy TrEMBL
Match: A0A6J1ER04 (E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111435772 PE=4 SV=1)

HSP 1 Score: 1386.7 bits (3588), Expect = 0.0e+00
Identity = 721/819 (88.03%), Postives = 760/819 (92.80%), Query Frame = 0

Query: 1   MEDEANRNLMLLPPPSPVVAVAISGKKNSKYIIRWSLEKFLPEGITDFKLLHIIPRITSV 60
           ME+EA  +L+ LP PSPVVAVAISGKKNS+YIIRWSLEKFLPEGI +FKLLH+ PRIT+V
Sbjct: 4   MEEEA--DLISLPSPSPVVAVAISGKKNSRYIIRWSLEKFLPEGIINFKLLHVTPRITTV 63

Query: 61  PTPMGNSIPISQVRDDVAAAYRKEIWWQTSEKLLPYKKMFAQRKVHLDVATLEADDVANA 120
           PTPMGNSIP+SQVRDDVAAAYRKEI WQT+EKLLPY KMFAQRKV LDV TLEADDVANA
Sbjct: 64  PTPMGNSIPVSQVRDDVAAAYRKEIGWQTNEKLLPYTKMFAQRKVQLDVVTLEADDVANA 123

Query: 121 IIEEVTKSSINKLVIGVSSQGLFSRKLNGLSSRISALAPRYCTVYAISKGKLASIRPPDM 180
           IIEEVTK SI+KLVIGVSSQG FSRKLNGLSSRISALAPR+CTVYAISKG+LASIRPPDM
Sbjct: 124 IIEEVTKCSISKLVIGVSSQGFFSRKLNGLSSRISALAPRHCTVYAISKGQLASIRPPDM 183

Query: 181 ETSVSIKDDASEASSANSYSSYSSSSLTDASSSLITSYSHFPSPSPSLPLQRFQALSTIN 240
           ET+VSIKDDASE+SSANSY SYSSSS+TD+SSSL TSYS FPS SPSLPLQRFQALSTIN
Sbjct: 184 ETNVSIKDDASESSSANSYLSYSSSSVTDSSSSLTTSYSDFPSSSPSLPLQRFQALSTIN 243

Query: 241 QSLLTTKPSPIKANHSRCQSVDIEDQVDGVRSSSYVSDCAQTLSRTSSCKSLPTDNQSWV 300
           Q+LLTTK SPIKA+HSRCQSVDIEDQVDGVRSSSYVSDC +TLSR SSCKSLP D QSWV
Sbjct: 244 QTLLTTKSSPIKADHSRCQSVDIEDQVDGVRSSSYVSDCVRTLSRDSSCKSLPMDGQSWV 303

Query: 301 SDEASSSGAFNDYSSSESQADVSFELEKLRIELRHARGMYAIAQRETIDASRKQEKKSHL 360
            DEASSSGAF D+ S ESQ DV+FELEKLRI+LRHARGMYAIAQRETIDASRK       
Sbjct: 304 -DEASSSGAFGDHVSCESQTDVNFELEKLRIKLRHARGMYAIAQRETIDASRK------- 363

Query: 361 VIVYDQLNHLNKQRSDEARKLEEINNKEVAAKEFAMEERMKREALRREAKYVKECAEREG 420
                 LNHLNKQRS++ARKL+EI N+EVAAKEFA EER KREALRREAKYVKE AEREG
Sbjct: 364 ------LNHLNKQRSEDARKLDEIKNREVAAKEFAREERTKREALRREAKYVKERAEREG 423

Query: 421 IYRKEAETKALQDAKEKGKHENALQGPLQQYQHFQWEDIVSATSSFSEDLKIGMGAHGTV 480
           IYRKEAETKALQDAKEKGKHENAL+GPLQQYQHFQWEDIVSATSSFSEDLK+GMGAHGTV
Sbjct: 424 IYRKEAETKALQDAKEKGKHENALEGPLQQYQHFQWEDIVSATSSFSEDLKLGMGAHGTV 483

Query: 481 YKCSLHHTTVAVKVLHSRDGHKRMQLLQELEILSRIHHPHLLLLLGACPDKNCLVYEYME 540
           YKCSLHHTTVAVKVLHSRD HK+ Q LQELEILS+IHHPHLLLLLGACPD NCLVYEYME
Sbjct: 484 YKCSLHHTTVAVKVLHSRDDHKKTQFLQELEILSKIHHPHLLLLLGACPDMNCLVYEYME 543

Query: 541 NGSLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDQNL 600
           NGSLEDRLY RGNTPAIPWYERFRIAWEIASALVFLHSSKPKPIIHRDLKPANILLD+NL
Sbjct: 544 NGSLEDRLYCRGNTPAIPWYERFRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNL 603

Query: 601 VSKIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQRTGLVSPKSDVYAFGMVILQ 660
           VSKIGDVGLSTVFNSDP MSTAF NSGPVGTLCYIDPEYQR+GL+SPKSDVYAFGMVILQ
Sbjct: 604 VSKIGDVGLSTVFNSDPLMSTAFKNSGPVGTLCYIDPEYQRSGLISPKSDVYAFGMVILQ 663

Query: 661 LLTAKPAVALTHVVETAIDNCTLINVLDIEAGHWPVEETCELARLGLRCAEMQRKDRPDL 720
           LLTAKPAVALTHVVETAIDNC+L  VLDIEAGHWPVEET ELARLGL CAEMQRKDRPDL
Sbjct: 664 LLTAKPAVALTHVVETAIDNCSLDKVLDIEAGHWPVEETYELARLGLHCAEMQRKDRPDL 723

Query: 721 KDQVLPLLMTLKKVAHEARNLASKVPAAIPNHFICPILQDMMNDPCVAADGYTYDRRAIE 780
           KD VLPLL+TLKKVA EAR+LASKVPA IPNHFICPILQD+MNDPCVAADGYTYDR AIE
Sbjct: 724 KDHVLPLLLTLKKVADEARSLASKVPAPIPNHFICPILQDVMNDPCVAADGYTYDRWAIE 783

Query: 781 KWFQENDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSRKN 820
           KW QENDNSP+TKLPLPDKNLIPN+SLLSAIVEWNSRKN
Sbjct: 784 KWLQENDNSPITKLPLPDKNLIPNFSLLSAIVEWNSRKN 806

BLAST of HG10009350 vs. ExPASy TrEMBL
Match: A0A6J1ET81 (E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111435772 PE=4 SV=1)

HSP 1 Score: 1376.3 bits (3561), Expect = 0.0e+00
Identity = 720/831 (86.64%), Postives = 760/831 (91.46%), Query Frame = 0

Query: 1   MEDEANRNLMLLPPPSPVVAVAISGKKNSKYIIRWSLEKFLPEGITDFKLLHIIPRITSV 60
           ME+EA  +L+ LP PSPVVAVAISGKKNS+YIIRWSLEKFLPEGI +FKLLH+ PRIT+V
Sbjct: 4   MEEEA--DLISLPSPSPVVAVAISGKKNSRYIIRWSLEKFLPEGIINFKLLHVTPRITTV 63

Query: 61  PTP------------MGNSIPISQVRDDVAAAYRKEIWWQTSEKLLPYKKMFAQRKVHLD 120
           PTP            +GNSIP+SQVRDDVAAAYRKEI WQT+EKLLPY KMFAQRKV LD
Sbjct: 64  PTPNSIFKLYTNVDAVGNSIPVSQVRDDVAAAYRKEIGWQTNEKLLPYTKMFAQRKVQLD 123

Query: 121 VATLEADDVANAIIEEVTKSSINKLVIGVSSQGLFSRKLNGLSSRISALAPRYCTVYAIS 180
           V TLEADDVANAIIEEVTK SI+KLVIGVSSQG FSRKLNGLSSRISALAPR+CTVYAIS
Sbjct: 124 VVTLEADDVANAIIEEVTKCSISKLVIGVSSQGFFSRKLNGLSSRISALAPRHCTVYAIS 183

Query: 181 KGKLASIRPPDMETSVSIKDDASEASSANSYSSYSSSSLTDASSSLITSYSHFPSPSPSL 240
           KG+LASIRPPDMET+VSIKDDASE+SSANSY SYSSSS+TD+SSSL TSYS FPS SPSL
Sbjct: 184 KGQLASIRPPDMETNVSIKDDASESSSANSYLSYSSSSVTDSSSSLTTSYSDFPSSSPSL 243

Query: 241 PLQRFQALSTINQSLLTTKPSPIKANHSRCQSVDIEDQVDGVRSSSYVSDCAQTLSRTSS 300
           PLQRFQALSTINQ+LLTTK SPIKA+HSRCQSVDIEDQVDGVRSSSYVSDC +TLSR SS
Sbjct: 244 PLQRFQALSTINQTLLTTKSSPIKADHSRCQSVDIEDQVDGVRSSSYVSDCVRTLSRDSS 303

Query: 301 CKSLPTDNQSWVSDEASSSGAFNDYSSSESQADVSFELEKLRIELRHARGMYAIAQRETI 360
           CKSLP D QSWV DEASSSGAF D+ S ESQ DV+FELEKLRI+LRHARGMYAIAQRETI
Sbjct: 304 CKSLPMDGQSWV-DEASSSGAFGDHVSCESQTDVNFELEKLRIKLRHARGMYAIAQRETI 363

Query: 361 DASRKQEKKSHLVIVYDQLNHLNKQRSDEARKLEEINNKEVAAKEFAMEERMKREALRRE 420
           DASRK             LNHLNKQRS++ARKL+EI N+EVAAKEFA EER KREALRRE
Sbjct: 364 DASRK-------------LNHLNKQRSEDARKLDEIKNREVAAKEFAREERTKREALRRE 423

Query: 421 AKYVKECAEREGIYRKEAETKALQDAKEKGKHENALQGPLQQYQHFQWEDIVSATSSFSE 480
           AKYVKE AEREGIYRKEAETKALQDAKEKGKHENAL+GPLQQYQHFQWEDIVSATSSFSE
Sbjct: 424 AKYVKERAEREGIYRKEAETKALQDAKEKGKHENALEGPLQQYQHFQWEDIVSATSSFSE 483

Query: 481 DLKIGMGAHGTVYKCSLHHTTVAVKVLHSRDGHKRMQLLQELEILSRIHHPHLLLLLGAC 540
           DLK+GMGAHGTVYKCSLHHTTVAVKVLHSRD HK+ Q LQELEILS+IHHPHLLLLLGAC
Sbjct: 484 DLKLGMGAHGTVYKCSLHHTTVAVKVLHSRDDHKKTQFLQELEILSKIHHPHLLLLLGAC 543

Query: 541 PDKNCLVYEYMENGSLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPKPIIHRD 600
           PD NCLVYEYMENGSLEDRLY RGNTPAIPWYERFRIAWEIASALVFLHSSKPKPIIHRD
Sbjct: 544 PDMNCLVYEYMENGSLEDRLYCRGNTPAIPWYERFRIAWEIASALVFLHSSKPKPIIHRD 603

Query: 601 LKPANILLDQNLVSKIGDVGLSTVFNSDPSMSTAFMNSGPVGTLCYIDPEYQRTGLVSPK 660
           LKPANILLD+NLVSKIGDVGLSTVFNSDP MSTAF NSGPVGTLCYIDPEYQR+GL+SPK
Sbjct: 604 LKPANILLDRNLVSKIGDVGLSTVFNSDPLMSTAFKNSGPVGTLCYIDPEYQRSGLISPK 663

Query: 661 SDVYAFGMVILQLLTAKPAVALTHVVETAIDNCTLINVLDIEAGHWPVEETCELARLGLR 720
           SDVYAFGMVILQLLTAKPAVALTHVVETAIDNC+L  VLDIEAGHWPVEET ELARLGL 
Sbjct: 664 SDVYAFGMVILQLLTAKPAVALTHVVETAIDNCSLDKVLDIEAGHWPVEETYELARLGLH 723

Query: 721 CAEMQRKDRPDLKDQVLPLLMTLKKVAHEARNLASKVPAAIPNHFICPILQDMMNDPCVA 780
           CAEMQRKDRPDLKD VLPLL+TLKKVA EAR+LASKVPA IPNHFICPILQD+MNDPCVA
Sbjct: 724 CAEMQRKDRPDLKDHVLPLLLTLKKVADEARSLASKVPAPIPNHFICPILQDVMNDPCVA 783

Query: 781 ADGYTYDRRAIEKWFQENDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSRKN 820
           ADGYTYDR AIEKW QENDNSP+TKLPLPDKNLIPN+SLLSAIVEWNSRKN
Sbjct: 784 ADGYTYDRWAIEKWLQENDNSPITKLPLPDKNLIPNFSLLSAIVEWNSRKN 818

BLAST of HG10009350 vs. TAIR 10
Match: AT4G25160.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 819.3 bits (2115), Expect = 2.7e-237
Identity = 451/838 (53.82%), Postives = 584/838 (69.69%), Query Frame = 0

Query: 13  PPPSPVVAVAISGKKNSKYIIRWSLEKFLPEGITDFKLLHIIPRITSVPTPMGNSIPISQ 72
           PPPS  V VA+SG   SKY++ W++EKF  EG   FKLLHI P ITSVPTPMGN+IPIS+
Sbjct: 16  PPPSRTVVVALSGSSKSKYVVTWAIEKFATEGNVGFKLLHIHPMITSVPTPMGNAIPISE 75

Query: 73  VRDDVAAAYRKEIWWQTSEKLLPYKKMFAQRKVHLDVATLEADDVANAIIEEVTKSSINK 132
           VRDDV  AYR+EI WQ+ E L PY K+F +RKV ++V  +E+D+VA AI EEVT+ SI++
Sbjct: 76  VRDDVVTAYRQEILWQSEEMLKPYTKLFVRRKVAVEVLVIESDNVAAAIAEEVTRDSIDR 135

Query: 133 LVIGVSSQGLFSRKLNGLSSRISALAPRYCTVYAISKGKLASIRPPDMETSVSIKDDASE 192
           +VIG SS+  FSRK + + S ISAL P +CTVY +SKGKL+ +RP D + + +I++D SE
Sbjct: 136 IVIGGSSRSFFSRKAD-ICSVISALMPNFCTVYVVSKGKLSCVRPSDSDGNATIREDGSE 195

Query: 193 ASSANSYSSYSSSSLTDASSSLITSYSHFPSPSPSLPLQRFQALSTI-NQSLLTTKPSPI 252
            ++++S SS  +S  +D  SS   S S       SLP++R Q    I  Q+ +  + S +
Sbjct: 196 RTNSSSGSSGPTSDSSDVMSSAHDSQSR----PLSLPVRRMQHFPAIAGQASVPMETSSV 255

Query: 253 KANHSRCQSVDIED--QVDGVRSSSYVSDCAQTLSR-----TSSCKSLPTDNQ------- 312
            ++ +RC S+D E+   V  +  SS  +    T  R          S  + N+       
Sbjct: 256 GSDETRCMSLDAEEARDVSSINRSSTDTTSRWTPRRRDYEERKEAMSSSSSNREYGNFGT 315

Query: 313 --SW-----------VSDEASS-SGAFNDYSSSESQADVSFELEKLRIELRHARGMYAIA 372
             SW            S +AS+ S A ++ S +++Q +++FE+EKLR ELRH + MYA+A
Sbjct: 316 RFSWSGMGVDTTHSRASQQASNMSDALSEQSYTDNQVNLNFEVEKLRAELRHVQEMYAVA 375

Query: 373 QRETIDASRKQEKKSHLVIVYDQLNHLNKQRSDEARKLEEINNKEVAAKEFAMEERMKRE 432
           Q ET DASRK             L  LN++R +EA KLEE+  KE  A+E A +E+   E
Sbjct: 376 QTETFDASRK-------------LGELNQRRLEEAIKLEELKLKEYEARELAEKEKQNFE 435

Query: 433 ALRREAKYVKECAEREGIYRKEAETKALQDAKEKGKHENALQGPLQQYQHFQWEDIVSAT 492
             RR+A+ ++E AERE   R+EAE K+ +D KEK K E  L  P  QYQHF WE+I++AT
Sbjct: 436 KARRDAESMRERAEREIAQRREAERKSARDTKEKEKLEGTLGSPQLQYQHFAWEEIMAAT 495

Query: 493 SSFSEDLKIGMGAHGTVYKCSLHHTTVAVKVLHSRDGHKRMQLLQELEILSRIHHPHLLL 552
           SSFSE+LKIGMGA+G VYKC+LHHTT  VKVL S +     Q  QELEILS+I HPHL+L
Sbjct: 496 SSFSEELKIGMGAYGAVYKCNLHHTTAVVKVLQSAENQLSKQFQQELEILSKIRHPHLVL 555

Query: 553 LLGACPDKNCLVYEYMENGSLEDRLYRRGNTPAIPWYERFRIAWEIASALVFLHSSKPKP 612
           LLGACP++  LVYEYMENGSLEDRL++  N+P +PW+ERFRIAWE+A+ALVFLH SKPKP
Sbjct: 556 LLGACPEQGALVYEYMENGSLEDRLFQVNNSPPLPWFERFRIAWEVAAALVFLHKSKPKP 615

Query: 613 IIHRDLKPANILLDQNLVSKIGDVGLSTVFNSDP--SMSTAFMNSGPVGTLCYIDPEYQR 672
           IIHRDLKPANILLD N VSK+GDVGLST+   DP  +  T +  + PVGTLCYIDPEYQR
Sbjct: 616 IIHRDLKPANILLDHNFVSKVGDVGLSTMVQVDPLSTKFTIYKQTSPVGTLCYIDPEYQR 675

Query: 673 TGLVSPKSDVYAFGMVILQLLTAKPAVALTHVVETAID-NCTLINVLDIEAGHWPVEETC 732
           TG +S KSD+Y+FGM++LQLLTAKPA+ALTH VE+A+D N   + +LD +AG+WP+EET 
Sbjct: 676 TGRISSKSDIYSFGMILLQLLTAKPAIALTHFVESAMDSNDEFLKILDQKAGNWPIEETR 735

Query: 733 ELARLGLRCAEMQRKDRPDLKDQVLPLLMTLKKVAHEARNLASKVPAAIPNHFICPILQD 792
           ELA L L C E++ KDRPDLKDQ+LP L  LKKVA +ARN  S V    P HFICP+L+D
Sbjct: 736 ELAALALCCTELRGKDRPDLKDQILPALENLKKVAEKARNSFSGVSTQPPTHFICPLLKD 795

Query: 793 MMNDPCVAADGYTYDRRAIEKWFQENDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSRK 819
           +MN+PCVAADGYTYDR AIE+W +E++ SPMT  PL  KNL+PNY+L +AI+EW S +
Sbjct: 796 VMNEPCVAADGYTYDRHAIEEWLKEHNTSPMTDSPLHSKNLLPNYTLYTAIMEWRSTR 835

BLAST of HG10009350 vs. TAIR 10
Match: AT5G61550.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 786.6 bits (2030), Expect = 2.0e-227
Identity = 429/848 (50.59%), Postives = 585/848 (68.99%), Query Frame = 0

Query: 6   NRNLMLLPPPSPVVAVAISGKKNSKYIIRWSLEKFLPEGITDFKLLHIIPRITSVPTPMG 65
           + +L L PPPSP VAVAI+GKK SKY++ W+LEKF+PEG TDFKLL++ P ++ +PTPMG
Sbjct: 11  SEHLSLPPPPSPSVAVAINGKKKSKYVVFWALEKFIPEGFTDFKLLYVRPPVSYIPTPMG 70

Query: 66  NSIPISQVRDDVAAAYRKEIWWQTSEKLLPYKKMFAQRKVHLDVATLEADDVANAIIEEV 125
            ++ +S++R+DV +AY++E+ W  +E L PYKKMF +RKV ++V  L++ + A AI EE+
Sbjct: 71  IAVAVSELREDVVSAYKQELDWSANEMLRPYKKMFERRKVQVEVLLLDSLEPAAAIAEEI 130

Query: 126 TKSSINKLVIGVSSQGLFSRKLNGLSSRISALAPRYCTVYAISKGKLASIRPPDMETSVS 185
             + + KLVIG+S +G FSRK++ +SS I+   PR+CTVY ISKGKLAS+RP + + S S
Sbjct: 131 AGTGVTKLVIGMSLRGFFSRKID-MSSLIATAVPRFCTVYVISKGKLASVRPSESDASGS 190

Query: 186 IKDDASEASSANSYSSYSSSSLTDASSSLITSYSHFPSPSPSLP-LQRFQALSTINQSLL 245
           I+ + S ++S ++ S        D  S++  + S     SP+L       A++ ++ S  
Sbjct: 191 IRFERSSSTSGSTDSPRLPPEYQDFLSAVSEAQSRVSPFSPALKHSMGSNAVAQMDTSSS 250

Query: 246 TTKPSPI---------------KANHSRCQSVDIEDQVDGVRSSSYVS------DCAQTL 305
            T    +               K N     S       +   S S+ S      D  +  
Sbjct: 251 GTDQEEVSTGRGMEIVHSGIEGKKNKDESFSASFPMGTEAYNSMSWTSKWRDHEDRREMR 310

Query: 306 SRTSSCK---------SLPTDNQSWVSDEAS--SSGAFNDYSSSESQADVSFELEKLRIE 365
           S +SS           ++  +N SWVS  AS  S G  + +S +++Q +++FE+EKLR E
Sbjct: 311 SSSSSNNHDLVNMDWGAVVPENYSWVSHTASHMSDGLLSVHSITDNQVNLNFEIEKLRAE 370

Query: 366 LRHARGMYAIAQRETIDASRKQEKKSHLVIVYDQLNHLNKQRSDEARKLEEINNKEVAAK 425
           L+H + MYA+AQ ET+ AS+K             L  LN++R +E+ KL E+  KE  AK
Sbjct: 371 LKHVQEMYAMAQTETVGASKK-------------LTELNQRRFEESEKLVELKEKEEVAK 430

Query: 426 EFAMEERMKREALRREAKYVKECAEREGIYRKEAETKALQDAKEKGKHENALQGPLQQYQ 485
           + A +E+ + E   +EA+ VKE   +E ++R+EAE KA +DA+EK K + +L  P  QYQ
Sbjct: 431 DTASKEKQRYEEAMKEAEKVKELMMKEALHRREAEFKAERDAREKDKLQASLVSPGVQYQ 490

Query: 486 HFQWEDIVSATSSFSEDLKIGMGAHGTVYKCSLHHTTVAVKVLHSRDGHKRMQLLQELEI 545
           H+ WE+I +ATS F+E+LKIG+GA+G+VYKC+LHHTT AVKVLH+ +     Q  QELEI
Sbjct: 491 HYTWEEIAAATSDFAENLKIGIGAYGSVYKCNLHHTTGAVKVLHAGETQLSKQFDQELEI 550

Query: 546 LSRIHHPHLLLLLGACPDKNCLVYEYMENGSLEDRLYRRGNTPAIPWYERFRIAWEIASA 605
           LS+I HPHL+LLLGACP++ CLVYEYM+NGSL+DRL    +TP IPW+ERFRIA E+ASA
Sbjct: 551 LSKIRHPHLVLLLGACPERGCLVYEYMDNGSLDDRLMLVNDTPPIPWFERFRIALEVASA 610

Query: 606 LVFLHSSKPKPIIHRDLKPANILLDQNLVSKIGDVGLSTVFNSDP-SMSTAFMNSGPVGT 665
           LVFLH SKP+PIIHRDLKP NILLD N VSK+GDVGLST+ N D  S  T F  + PVGT
Sbjct: 611 LVFLHKSKPRPIIHRDLKPGNILLDHNFVSKLGDVGLSTMVNQDDVSSRTIFKQTSPVGT 670

Query: 666 LCYIDPEYQRTGLVSPKSDVYAFGMVILQLLTAKPAVALTHVVETAI-DNCTLINVLDIE 725
           LCYIDPEYQRTG++SPKSDVY+ G+VILQL+TAKPA+A+TH+VE AI D+   + +LD +
Sbjct: 671 LCYIDPEYQRTGIISPKSDVYSLGVVILQLITAKPAIAITHMVEEAIGDDAEFMAILDKK 730

Query: 726 AGHWPVEETCELARLGLRCAEMQRKDRPDLKDQVLPLLMTLKKVAHEARNLASKVPAAIP 785
           AG WP+ +T ELA LGL C EM+R+DRPDLKDQ++P L  L+KVA +A+NL S+ P+  P
Sbjct: 731 AGSWPISDTRELAALGLCCTEMRRRDRPDLKDQIIPALERLRKVADKAQNLLSRTPSGPP 790

Query: 786 NHFICPILQDMMNDPCVAADGYTYDRRAIEKWFQENDNSPMTKLPLPDKNLIPNYSLLSA 819
           +HFICP+L+ +MN+PCVAADGYTYDR AIE+W ++ D SP+T LPLP+KNLI NY+L SA
Sbjct: 791 SHFICPLLKGVMNEPCVAADGYTYDREAIEEWLRQKDTSPVTNLPLPNKNLIANYTLYSA 844

BLAST of HG10009350 vs. TAIR 10
Match: AT5G61550.2 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 786.6 bits (2030), Expect = 2.0e-227
Identity = 429/848 (50.59%), Postives = 585/848 (68.99%), Query Frame = 0

Query: 6   NRNLMLLPPPSPVVAVAISGKKNSKYIIRWSLEKFLPEGITDFKLLHIIPRITSVPTPMG 65
           + +L L PPPSP VAVAI+GKK SKY++ W+LEKF+PEG TDFKLL++ P ++ +PTPMG
Sbjct: 11  SEHLSLPPPPSPSVAVAINGKKKSKYVVFWALEKFIPEGFTDFKLLYVRPPVSYIPTPMG 70

Query: 66  NSIPISQVRDDVAAAYRKEIWWQTSEKLLPYKKMFAQRKVHLDVATLEADDVANAIIEEV 125
            ++ +S++R+DV +AY++E+ W  +E L PYKKMF +RKV ++V  L++ + A AI EE+
Sbjct: 71  IAVAVSELREDVVSAYKQELDWSANEMLRPYKKMFERRKVQVEVLLLDSLEPAAAIAEEI 130

Query: 126 TKSSINKLVIGVSSQGLFSRKLNGLSSRISALAPRYCTVYAISKGKLASIRPPDMETSVS 185
             + + KLVIG+S +G FSRK++ +SS I+   PR+CTVY ISKGKLAS+RP + + S S
Sbjct: 131 AGTGVTKLVIGMSLRGFFSRKID-MSSLIATAVPRFCTVYVISKGKLASVRPSESDASGS 190

Query: 186 IKDDASEASSANSYSSYSSSSLTDASSSLITSYSHFPSPSPSLP-LQRFQALSTINQSLL 245
           I+ + S ++S ++ S        D  S++  + S     SP+L       A++ ++ S  
Sbjct: 191 IRFERSSSTSGSTDSPRLPPEYQDFLSAVSEAQSRVSPFSPALKHSMGSNAVAQMDTSSS 250

Query: 246 TTKPSPI---------------KANHSRCQSVDIEDQVDGVRSSSYVS------DCAQTL 305
            T    +               K N     S       +   S S+ S      D  +  
Sbjct: 251 GTDQEEVSTGRGMEIVHSGIEGKKNKDESFSASFPMGTEAYNSMSWTSKWRDHEDRREMR 310

Query: 306 SRTSSCK---------SLPTDNQSWVSDEAS--SSGAFNDYSSSESQADVSFELEKLRIE 365
           S +SS           ++  +N SWVS  AS  S G  + +S +++Q +++FE+EKLR E
Sbjct: 311 SSSSSNNHDLVNMDWGAVVPENYSWVSHTASHMSDGLLSVHSITDNQVNLNFEIEKLRAE 370

Query: 366 LRHARGMYAIAQRETIDASRKQEKKSHLVIVYDQLNHLNKQRSDEARKLEEINNKEVAAK 425
           L+H + MYA+AQ ET+ AS+K             L  LN++R +E+ KL E+  KE  AK
Sbjct: 371 LKHVQEMYAMAQTETVGASKK-------------LTELNQRRFEESEKLVELKEKEEVAK 430

Query: 426 EFAMEERMKREALRREAKYVKECAEREGIYRKEAETKALQDAKEKGKHENALQGPLQQYQ 485
           + A +E+ + E   +EA+ VKE   +E ++R+EAE KA +DA+EK K + +L  P  QYQ
Sbjct: 431 DTASKEKQRYEEAMKEAEKVKELMMKEALHRREAEFKAERDAREKDKLQASLVSPGVQYQ 490

Query: 486 HFQWEDIVSATSSFSEDLKIGMGAHGTVYKCSLHHTTVAVKVLHSRDGHKRMQLLQELEI 545
           H+ WE+I +ATS F+E+LKIG+GA+G+VYKC+LHHTT AVKVLH+ +     Q  QELEI
Sbjct: 491 HYTWEEIAAATSDFAENLKIGIGAYGSVYKCNLHHTTGAVKVLHAGETQLSKQFDQELEI 550

Query: 546 LSRIHHPHLLLLLGACPDKNCLVYEYMENGSLEDRLYRRGNTPAIPWYERFRIAWEIASA 605
           LS+I HPHL+LLLGACP++ CLVYEYM+NGSL+DRL    +TP IPW+ERFRIA E+ASA
Sbjct: 551 LSKIRHPHLVLLLGACPERGCLVYEYMDNGSLDDRLMLVNDTPPIPWFERFRIALEVASA 610

Query: 606 LVFLHSSKPKPIIHRDLKPANILLDQNLVSKIGDVGLSTVFNSDP-SMSTAFMNSGPVGT 665
           LVFLH SKP+PIIHRDLKP NILLD N VSK+GDVGLST+ N D  S  T F  + PVGT
Sbjct: 611 LVFLHKSKPRPIIHRDLKPGNILLDHNFVSKLGDVGLSTMVNQDDVSSRTIFKQTSPVGT 670

Query: 666 LCYIDPEYQRTGLVSPKSDVYAFGMVILQLLTAKPAVALTHVVETAI-DNCTLINVLDIE 725
           LCYIDPEYQRTG++SPKSDVY+ G+VILQL+TAKPA+A+TH+VE AI D+   + +LD +
Sbjct: 671 LCYIDPEYQRTGIISPKSDVYSLGVVILQLITAKPAIAITHMVEEAIGDDAEFMAILDKK 730

Query: 726 AGHWPVEETCELARLGLRCAEMQRKDRPDLKDQVLPLLMTLKKVAHEARNLASKVPAAIP 785
           AG WP+ +T ELA LGL C EM+R+DRPDLKDQ++P L  L+KVA +A+NL S+ P+  P
Sbjct: 731 AGSWPISDTRELAALGLCCTEMRRRDRPDLKDQIIPALERLRKVADKAQNLLSRTPSGPP 790

Query: 786 NHFICPILQDMMNDPCVAADGYTYDRRAIEKWFQENDNSPMTKLPLPDKNLIPNYSLLSA 819
           +HFICP+L+ +MN+PCVAADGYTYDR AIE+W ++ D SP+T LPLP+KNLI NY+L SA
Sbjct: 791 SHFICPLLKGVMNEPCVAADGYTYDREAIEEWLRQKDTSPVTNLPLPNKNLIANYTLYSA 844

BLAST of HG10009350 vs. TAIR 10
Match: AT5G61560.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 689.1 bits (1777), Expect = 4.3e-198
Identity = 405/827 (48.97%), Postives = 547/827 (66.14%), Query Frame = 0

Query: 18  VVAVAISGKKN-SKYIIRWSLEKFLPEGITDFKLLHIIPRITSVPTPMGNSIPISQVRDD 77
           +VAVAI G  + +K ++RW+L++F  +    FKLLH+ PR         +S  +S  R D
Sbjct: 7   IVAVAIKGNNSKTKGVVRWALQEFASQEHVVFKLLHVQPR---------DSNSVSTTRKD 66

Query: 78  V-AAAYRKEIWWQTSEKLLPYKKMFAQRKVHLDVATLEADDVANAIIEEVTKSSINKLVI 137
           +  + Y+K++  +T E LLP + MF  R+V LD+  LE+DD+A+AI + V    I++LVI
Sbjct: 67  LTTSVYKKDVDRKTREMLLPSRDMFVHREVQLDIMVLESDDIADAISKAVQDHGISELVI 126

Query: 138 GVSSQGLFSRKL--NGLSSRISALAPRYCTVYAISKGKLASIRPPDMETSVSIKDDASEA 197
           G SS  +FS KL  + LSSRI+   PR+C+V+ ISKGKL ++R  DM+T  SI DD SE 
Sbjct: 127 GASSSIIFSWKLKRSNLSSRIADATPRFCSVHVISKGKLLNVRKSDMDTETSIADDRSE- 186

Query: 198 SSANSYSSYSSSSLTDASSSLITSYSHFPSPSPSLPLQRFQALSTINQSLLTT----KPS 257
                 S +SS    D+ S  ++S S     S  L  QR QAL+T+NQ + T        
Sbjct: 187 ------SRFSS----DSHSGTVSSTSSHQFSSTPLLFQRIQALTTVNQKVGTNIGKQNNE 246

Query: 258 PIKANHSRCQSVDIEDQVDGVRSSSYVSDCAQTLSRTSSCKSLPTDNQSWVS---DEASS 317
           P   +H+R  S+D+++       S  ++      + +S      +D  SW S   +EASS
Sbjct: 247 PHHHHHNRAGSLDVDE-------SKLLNQKGFYRTSSSGIGYGGSDISSWRSSQMEEASS 306

Query: 318 SGAFNDYSSSESQADVSFELEKLRIELRHARGMYAIAQRETIDASRKQEKKSHLVIVYDQ 377
           S  ++D +SS SQ    FELEKL+IELRH +GMYA+AQ E IDAS+K             
Sbjct: 307 SSTYSDPTSSSSQIHKDFELEKLKIELRHIKGMYAVAQSEVIDASKK------------- 366

Query: 378 LNHLNKQRSDEARKLEEINNKEVAAKEFAMEERMKREALRREAKYVKECAEREGIYRKEA 437
           +  LN++RS+EA +L+ +  +E  A E    ER ++E    EA+ V+EC ERE   R EA
Sbjct: 367 MQDLNQRRSEEATRLKNLTIREEEADEVVEMERERQEDAENEAELVRECIERETEERLEA 426

Query: 438 ETKALQDAKEKGKHENALQ-GPL--QQYQHFQWEDIVSATSSFSEDLKIGMGAHGTVYKC 497
           E +A +  KEK + E+AL+ GPL  QQY  F+WE+IV ATSSFS++LKIG+G +G+VY+C
Sbjct: 427 EARAEEVRKEKQRLEDALEGGPLQRQQYMKFEWEEIVEATSSFSDELKIGVGGYGSVYRC 486

Query: 498 SLHHTTVAVKVLHSRDGHKRMQLLQELEILSRIHHPHLLLLLGACPDKNCLVYEYMENGS 557
           +LHHTTVAVKVLHS       Q  QELEILS+I HPHLLLLLGACP++  LVYEYM NGS
Sbjct: 487 NLHHTTVAVKVLHSDKSSLTKQFHQELEILSKIRHPHLLLLLGACPERGSLVYEYMHNGS 546

Query: 558 LEDRLYRR------GNTPAIPWYERFRIAWEIASALVFLHSSKPKPIIHRDLKPANILLD 617
           LE+RL +R         P + W+ERFRIAWEIASAL FLH+++P+PI+HRDLKPANILLD
Sbjct: 547 LEERLMKRRPNVDTPQPPPLRWFERFRIAWEIASALYFLHTNEPRPIVHRDLKPANILLD 606

Query: 618 QNLVSKIGDVGLSTVFNSDPS-MSTAFMNSGPVGTLCYIDPEYQRTGLVSPKSDVYAFGM 677
           +N VSKIGDVGLS + N DPS  ST F  +GPVGT  YIDPEYQRTG+V+P+SD+YAFG+
Sbjct: 607 RNNVSKIGDVGLSKMVNLDPSHASTVFNETGPVGTFFYIDPEYQRTGVVTPESDIYAFGI 666

Query: 678 VILQLLTAKPAVALTHVVETAIDNCT--LINVLDIEAGHWPVEETCELARLGLRCAEMQR 737
           ++LQL+TA+ A+ L H +E A+ + T     +LD  AG WPV+E  E+  +GLRCAEM++
Sbjct: 667 ILLQLVTARSAMGLAHSIEKALRDQTGKFTEILDKTAGDWPVKEAKEMVMIGLRCAEMRK 726

Query: 738 KDRPDLKDQVLPLLMTLKKVAHEARNLAS----KVPAAIPNHFICPILQDMMNDPCVAAD 797
           +DRPDL  ++LP+L  LK+VA  ARN+ +          P HF CPI +D+M +PCVA+D
Sbjct: 727 RDRPDLGKEILPVLERLKEVASIARNMFADNLIDHHHNAPTHFYCPITKDVMENPCVASD 786

Query: 798 GYTYDRRAIEKWFQENDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSR 818
           GYTY++RAI++W Q+N  SPMT LP P  +L+PN+SLLSAI EW S+
Sbjct: 787 GYTYEKRAIKEWLQKNHKSPMTDLPFPSDSLLPNHSLLSAIKEWRSQ 793

BLAST of HG10009350 vs. TAIR 10
Match: AT5G61560.2 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 688.0 bits (1774), Expect = 9.5e-198
Identity = 405/827 (48.97%), Postives = 548/827 (66.26%), Query Frame = 0

Query: 18  VVAVAISGKKN-SKYIIRWSLEKFLPEGITDFKLLHIIPRITSVPTPMGNSIPISQVRDD 77
           +VAVAI G  + +K ++RW+L++F  +    FKLLH+ PR         +S  +S  R D
Sbjct: 7   IVAVAIKGNNSKTKGVVRWALQEFASQEHVVFKLLHVQPR---------DSNSVSTTRKD 66

Query: 78  V-AAAYRKEIWWQTSEKLLPYKKMFAQRKVHLDVATLEADDVANAIIEEVTKSSINKLVI 137
           +  + Y+K++  +T E LLP + MF  R+V LD+  LE+DD+A+AI + V    I++LVI
Sbjct: 67  LTTSVYKKDVDRKTREMLLPSRDMFVHREVQLDIMVLESDDIADAISKAVQDHGISELVI 126

Query: 138 GVSSQGLFSRKL--NGLSSRISALAPRYCTVYAISKGKLASIRPPDMETSVSIKDDASEA 197
           G SS  +FS KL  + LSSRI+   PR+C+V+ ISKGKL ++R  DM+T  SI DD SE+
Sbjct: 127 GASSSIIFSWKLKRSNLSSRIADATPRFCSVHVISKGKLLNVRKSDMDTETSIADDRSES 186

Query: 198 SSANSYSSYSSSSLTDASSSLITSYSHFPSPSPSLPLQRFQALSTINQSLLTT----KPS 257
             ++   S SS+S            SH  S +P L  QR QAL+T+NQ + T        
Sbjct: 187 RFSSDSHSVSSTS------------SHQFSSTPLL-FQRIQALTTVNQKVGTNIGKQNNE 246

Query: 258 PIKANHSRCQSVDIEDQVDGVRSSSYVSDCAQTLSRTSSCKSLPTDNQSWVS---DEASS 317
           P   +H+R  S+D+++       S  ++      + +S      +D  SW S   +EASS
Sbjct: 247 PHHHHHNRAGSLDVDE-------SKLLNQKGFYRTSSSGIGYGGSDISSWRSSQMEEASS 306

Query: 318 SGAFNDYSSSESQADVSFELEKLRIELRHARGMYAIAQRETIDASRKQEKKSHLVIVYDQ 377
           S  ++D +SS SQ    FELEKL+IELRH +GMYA+AQ E IDAS+K             
Sbjct: 307 SSTYSDPTSSSSQIHKDFELEKLKIELRHIKGMYAVAQSEVIDASKK------------- 366

Query: 378 LNHLNKQRSDEARKLEEINNKEVAAKEFAMEERMKREALRREAKYVKECAEREGIYRKEA 437
           +  LN++RS+EA +L+ +  +E  A E    ER ++E    EA+ V+EC ERE   R EA
Sbjct: 367 MQDLNQRRSEEATRLKNLTIREEEADEVVEMERERQEDAENEAELVRECIERETEERLEA 426

Query: 438 ETKALQDAKEKGKHENALQ-GPL--QQYQHFQWEDIVSATSSFSEDLKIGMGAHGTVYKC 497
           E +A +  KEK + E+AL+ GPL  QQY  F+WE+IV ATSSFS++LKIG+G +G+VY+C
Sbjct: 427 EARAEEVRKEKQRLEDALEGGPLQRQQYMKFEWEEIVEATSSFSDELKIGVGGYGSVYRC 486

Query: 498 SLHHTTVAVKVLHSRDGHKRMQLLQELEILSRIHHPHLLLLLGACPDKNCLVYEYMENGS 557
           +LHHTTVAVKVLHS       Q  QELEILS+I HPHLLLLLGACP++  LVYEYM NGS
Sbjct: 487 NLHHTTVAVKVLHSDKSSLTKQFHQELEILSKIRHPHLLLLLGACPERGSLVYEYMHNGS 546

Query: 558 LEDRLYRR------GNTPAIPWYERFRIAWEIASALVFLHSSKPKPIIHRDLKPANILLD 617
           LE+RL +R         P + W+ERFRIAWEIASAL FLH+++P+PI+HRDLKPANILLD
Sbjct: 547 LEERLMKRRPNVDTPQPPPLRWFERFRIAWEIASALYFLHTNEPRPIVHRDLKPANILLD 606

Query: 618 QNLVSKIGDVGLSTVFNSDPS-MSTAFMNSGPVGTLCYIDPEYQRTGLVSPKSDVYAFGM 677
           +N VSKIGDVGLS + N DPS  ST F  +GPVGT  YIDPEYQRTG+V+P+SD+YAFG+
Sbjct: 607 RNNVSKIGDVGLSKMVNLDPSHASTVFNETGPVGTFFYIDPEYQRTGVVTPESDIYAFGI 666

Query: 678 VILQLLTAKPAVALTHVVETAIDNCT--LINVLDIEAGHWPVEETCELARLGLRCAEMQR 737
           ++LQL+TA+ A+ L H +E A+ + T     +LD  AG WPV+E  E+  +GLRCAEM++
Sbjct: 667 ILLQLVTARSAMGLAHSIEKALRDQTGKFTEILDKTAGDWPVKEAKEMVMIGLRCAEMRK 726

Query: 738 KDRPDLKDQVLPLLMTLKKVAHEARNLAS----KVPAAIPNHFICPILQDMMNDPCVAAD 797
           +DRPDL  ++LP+L  LK+VA  ARN+ +          P HF CPI +D+M +PCVA+D
Sbjct: 727 RDRPDLGKEILPVLERLKEVASIARNMFADNLIDHHHNAPTHFYCPITKDVMENPCVASD 786

Query: 798 GYTYDRRAIEKWFQENDNSPMTKLPLPDKNLIPNYSLLSAIVEWNSR 818
           GYTY++RAI++W Q+N  SPMT LP P  +L+PN+SLLSAI EW S+
Sbjct: 787 GYTYEKRAIKEWLQKNHKSPMTDLPFPSDSLLPNHSLLSAIKEWRSQ 791

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038877140.10.0e+0092.80U-box domain-containing protein 35-like isoform X2 [Benincasa hispida][more]
XP_038877139.10.0e+0091.34U-box domain-containing protein 35-like isoform X1 [Benincasa hispida][more]
XP_008437017.10.0e+0089.84PREDICTED: U-box domain-containing protein 35-like isoform X2 [Cucumis melo][more]
XP_011654848.10.0e+0089.84U-box domain-containing protein 35 isoform X3 [Cucumis sativus] >KGN49786.2 hypo... [more]
XP_011654847.10.0e+0089.39U-box domain-containing protein 35 isoform X2 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q9SW113.9e-23653.82U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2... [more]
Q9FKG62.8e-22650.59U-box domain-containing protein 52 OS=Arabidopsis thaliana OX=3702 GN=PUB52 PE=2... [more]
Q9FKG56.0e-19748.97U-box domain-containing protein 51 OS=Arabidopsis thaliana OX=3702 GN=PUB51 PE=2... [more]
Q9LU471.5e-18447.01Putative U-box domain-containing protein 53 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q8S8S71.9e-11334.24U-box domain-containing protein 34 OS=Arabidopsis thaliana OX=3702 GN=PUB34 PE=3... [more]
Match NameE-valueIdentityDescription
A0A1S3ATK70.0e+0089.84E3 ubiquitin ligase OS=Cucumis melo OX=3656 GN=LOC103482570 PE=4 SV=1[more]
A0A0A0KL010.0e+0089.84E3 ubiquitin ligase OS=Cucumis sativus OX=3659 GN=Csa_5G167050 PE=4 SV=1[more]
A0A1S4DSC40.0e+0088.42E3 ubiquitin ligase OS=Cucumis melo OX=3656 GN=LOC103482570 PE=4 SV=1[more]
A0A6J1ER040.0e+0088.03E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111435772 PE=4 SV=1[more]
A0A6J1ET810.0e+0086.64E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111435772 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G25160.12.7e-23753.82U-box domain-containing protein kinase family protein [more]
AT5G61550.12.0e-22750.59U-box domain-containing protein kinase family protein [more]
AT5G61550.22.0e-22750.59U-box domain-containing protein kinase family protein [more]
AT5G61560.14.3e-19848.97U-box domain-containing protein kinase family protein [more]
AT5G61560.29.5e-19848.97U-box domain-containing protein kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 326..346
NoneNo IPR availableCOILSCoilCoilcoord: 367..387
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 539..747
e-value: 1.3E-46
score: 160.5
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 435..538
e-value: 1.4E-22
score: 81.8
NoneNo IPR availablePIRSRPIRSR037921-1PIRSR037921-1coord: 472..665
e-value: 2.4E-32
score: 110.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 294..314
NoneNo IPR availablePANTHERPTHR45647OS02G0152300 PROTEINcoord: 16..815
NoneNo IPR availablePANTHERPTHR45647:SF15U-BOX DOMAIN-CONTAINING PROTEIN 35coord: 16..815
NoneNo IPR availableCDDcd16655RING-Ubox_WDSUB1_likecoord: 753..793
e-value: 2.06477E-20
score: 83.0371
NoneNo IPR availableCDDcd01989STK_Ncoord: 18..170
e-value: 1.6008E-44
score: 155.127
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 742..817
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 19..158
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 466..728
e-value: 1.0E-27
score: 108.1
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 468..720
e-value: 2.8E-40
score: 138.4
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 466..728
score: 33.742817
IPR003613U box domainSMARTSM00504Ubox_2coord: 752..814
e-value: 6.4E-28
score: 108.8
IPR003613U box domainPFAMPF04564U-boxcoord: 749..818
e-value: 3.1E-18
score: 65.7
IPR003613U box domainPROSITEPS51698U_BOXcoord: 748..819
score: 34.67691
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 748..819
e-value: 7.3E-28
score: 98.3
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 14..167
e-value: 1.8E-5
score: 27.1
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 584..596
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 472..493
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 447..722

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10009350.1HG10009350.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004842 ubiquitin-protein transferase activity
molecular_function GO:0004672 protein kinase activity