HG10009250 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10009250
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionIRK-interacting protein
LocationChr06: 3994829 .. 3996399 (-)
RNA-Seq ExpressionHG10009250
SyntenyHG10009250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCTGCTTCTTCACCCACTTCCTTCAATTCTTCTTCTTCTTCTTCTTCCTTTTCTCTTCCCACCCACCACCACTCTCCATCCCATTTCACGCCGGTGAGTCTTTTACACCACAATTTCTAAAATTTCCCCTGTTTTTTTTTTCTCCGCAAAATGATTGAGTTTTATTGCAGATTCAAGAATGCGACAGGGAAGAGCGGGAGGATAGCATTCCTGCTGAGAATCGGACTCAGATTACAGCCGCCGCGACTGGCGGATGTACGGACCGGGGAACGAGTCCGAAGTACCACCCGACGCCACTAAATCGGAGTAAGAATGGAAAATCACAGACGGTTAAGAAACGGTCGGAGTCGGAGTCGGATTCCGTGTCGAGTTCCGACGGGCCGGTGTCGTGTAACCGTTGCCGTCCTCACGCGCGGGAGAAGATCTCCGTTGTTCCTCTGGACAACAACAATGGCGTCAATAAACAAACCTATTTCTCCATGGCGAGTCCTAATGGCATTTTCAAATCCTTAATTTCGTCATTAACGCGAAAGAGTCCGAAATCGATAAACGAATCTTCGGCCCTAACGGCTCGAGAAGAGCAATGGAGGACCGCCGTGACGGAGCTGTCGCAGAAGCTAGTTCAGGCGACAAGGAAGAGAGACGAGGCTATCATGGAAGCTTCTAGATTAAAATACGCCATGGCTGAACTGGAGAAGAAACTGGACAAACTCGAGACGTATTGCCACAGTTTGAAATCCGGGATCGAAGAATGCAGCGGCAATTCACCATCGCCTTGCCAAATTGGGAAGTATAATCAAATCCAGAGTTTTCAACAGAGGAATCAGAAGCAAGTAATAGAACATTTCTTAGTTTCAGTATCGGAATCTCGATCGTCGATTCGGCTTCTAAGCCGGTCACTCACTCTGCAACTCCGCCACGTCGGAGCCAAAGTATACGAACGAATTTCCGTTCTTCTTCAACCTTACGATATCAAAATCTCATTCTCCAAGAACCCTAGAAGCATGCTTTTCTACCTGGAAGCTCTGCTCAACCAAGCTTTCTTCGAGGATTTCGAATCGATAGGGTTTCAAAAGAACGCATCGACTCAGGTTCTAAATCCAATTGAAAGATGCGAAGCGAATTTCGGATGTTTCAATTTACTCCATGAATTAACGTGGGAGGAGGTTCTAAGCAAAGGCACGAAGCATTTCAGCGAGGATTTCAGCCGCTTTTGTGACCGGAAAATGAGCGAAATCGTCGCGATGCTCGGATGGAACAGAGCCTGGCCGGAGCCGTTGCTGCAGGCGTTCTTCAGCGCGTCGAAGAGCGTGTGGCTTCTGCACCTACTGGCGAACTCCGTACATCCGAATCTGCCGATTTTCAGAGTGGATAAAGAGGCCGATTTCGATTCCGTTTACATGGAGGACATGGGCGGCGATAAGGCCAGAAAGCTGATACCGTCGTTGGTCAGAATAATGATTGCCCCTGGTTTCTATGTTTACGGCAGTGTAGTTAAATGCAAGGTGTTGTGCAGGTATAACGCCGCCGCCTCCGCCGTAACCGCCGCCGCATTGTCACCGTAA

mRNA sequence

ATGGCTGCTGCTTCTTCACCCACTTCCTTCAATTCTTCTTCTTCTTCTTCTTCCTTTTCTCTTCCCACCCACCACCACTCTCCATCCCATTTCACGCCGATTCAAGAATGCGACAGGGAAGAGCGGGAGGATAGCATTCCTGCTGAGAATCGGACTCAGATTACAGCCGCCGCGACTGGCGGATGTACGGACCGGGGAACGAGTCCGAAGTACCACCCGACGCCACTAAATCGGAGTAAGAATGGAAAATCACAGACGGTTAAGAAACGGTCGGAGTCGGAGTCGGATTCCGTGTCGAGTTCCGACGGGCCGGTGTCGTGTAACCGTTGCCGTCCTCACGCGCGGGAGAAGATCTCCGTTGTTCCTCTGGACAACAACAATGGCGTCAATAAACAAACCTATTTCTCCATGGCGAGTCCTAATGGCATTTTCAAATCCTTAATTTCGTCATTAACGCGAAAGAGTCCGAAATCGATAAACGAATCTTCGGCCCTAACGGCTCGAGAAGAGCAATGGAGGACCGCCGTGACGGAGCTGTCGCAGAAGCTAGTTCAGGCGACAAGGAAGAGAGACGAGGCTATCATGGAAGCTTCTAGATTAAAATACGCCATGGCTGAACTGGAGAAGAAACTGGACAAACTCGAGACGTATTGCCACAGTTTGAAATCCGGGATCGAAGAATGCAGCGGCAATTCACCATCGCCTTGCCAAATTGGGAAGTATAATCAAATCCAGAGTTTTCAACAGAGGAATCAGAAGCAAGTAATAGAACATTTCTTAGTTTCAGTATCGGAATCTCGATCGTCGATTCGGCTTCTAAGCCGGTCACTCACTCTGCAACTCCGCCACGTCGGAGCCAAAGTATACGAACGAATTTCCGTTCTTCTTCAACCTTACGATATCAAAATCTCATTCTCCAAGAACCCTAGAAGCATGCTTTTCTACCTGGAAGCTCTGCTCAACCAAGCTTTCTTCGAGGATTTCGAATCGATAGGGTTTCAAAAGAACGCATCGACTCAGGTTCTAAATCCAATTGAAAGATGCGAAGCGAATTTCGGATGTTTCAATTTACTCCATGAATTAACGTGGGAGGAGGTTCTAAGCAAAGGCACGAAGCATTTCAGCGAGGATTTCAGCCGCTTTTGTGACCGGAAAATGAGCGAAATCGTCGCGATGCTCGGATGGAACAGAGCCTGGCCGGAGCCGTTGCTGCAGGCGTTCTTCAGCGCGTCGAAGAGCGTGTGGCTTCTGCACCTACTGGCGAACTCCGTACATCCGAATCTGCCGATTTTCAGAGTGGATAAAGAGGCCGATTTCGATTCCGTTTACATGGAGGACATGGGCGGCGATAAGGCCAGAAAGCTGATACCGTCGTTGGTCAGAATAATGATTGCCCCTGGTTTCTATGTTTACGGCAGTGTAGTTAAATGCAAGGTGTTGTGCAGGTATAACGCCGCCGCCTCCGCCGTAACCGCCGCCGCATTGTCACCGTAA

Coding sequence (CDS)

ATGGCTGCTGCTTCTTCACCCACTTCCTTCAATTCTTCTTCTTCTTCTTCTTCCTTTTCTCTTCCCACCCACCACCACTCTCCATCCCATTTCACGCCGATTCAAGAATGCGACAGGGAAGAGCGGGAGGATAGCATTCCTGCTGAGAATCGGACTCAGATTACAGCCGCCGCGACTGGCGGATGTACGGACCGGGGAACGAGTCCGAAGTACCACCCGACGCCACTAAATCGGAGTAAGAATGGAAAATCACAGACGGTTAAGAAACGGTCGGAGTCGGAGTCGGATTCCGTGTCGAGTTCCGACGGGCCGGTGTCGTGTAACCGTTGCCGTCCTCACGCGCGGGAGAAGATCTCCGTTGTTCCTCTGGACAACAACAATGGCGTCAATAAACAAACCTATTTCTCCATGGCGAGTCCTAATGGCATTTTCAAATCCTTAATTTCGTCATTAACGCGAAAGAGTCCGAAATCGATAAACGAATCTTCGGCCCTAACGGCTCGAGAAGAGCAATGGAGGACCGCCGTGACGGAGCTGTCGCAGAAGCTAGTTCAGGCGACAAGGAAGAGAGACGAGGCTATCATGGAAGCTTCTAGATTAAAATACGCCATGGCTGAACTGGAGAAGAAACTGGACAAACTCGAGACGTATTGCCACAGTTTGAAATCCGGGATCGAAGAATGCAGCGGCAATTCACCATCGCCTTGCCAAATTGGGAAGTATAATCAAATCCAGAGTTTTCAACAGAGGAATCAGAAGCAAGTAATAGAACATTTCTTAGTTTCAGTATCGGAATCTCGATCGTCGATTCGGCTTCTAAGCCGGTCACTCACTCTGCAACTCCGCCACGTCGGAGCCAAAGTATACGAACGAATTTCCGTTCTTCTTCAACCTTACGATATCAAAATCTCATTCTCCAAGAACCCTAGAAGCATGCTTTTCTACCTGGAAGCTCTGCTCAACCAAGCTTTCTTCGAGGATTTCGAATCGATAGGGTTTCAAAAGAACGCATCGACTCAGGTTCTAAATCCAATTGAAAGATGCGAAGCGAATTTCGGATGTTTCAATTTACTCCATGAATTAACGTGGGAGGAGGTTCTAAGCAAAGGCACGAAGCATTTCAGCGAGGATTTCAGCCGCTTTTGTGACCGGAAAATGAGCGAAATCGTCGCGATGCTCGGATGGAACAGAGCCTGGCCGGAGCCGTTGCTGCAGGCGTTCTTCAGCGCGTCGAAGAGCGTGTGGCTTCTGCACCTACTGGCGAACTCCGTACATCCGAATCTGCCGATTTTCAGAGTGGATAAAGAGGCCGATTTCGATTCCGTTTACATGGAGGACATGGGCGGCGATAAGGCCAGAAAGCTGATACCGTCGTTGGTCAGAATAATGATTGCCCCTGGTTTCTATGTTTACGGCAGTGTAGTTAAATGCAAGGTGTTGTGCAGGTATAACGCCGCCGCCTCCGCCGTAACCGCCGCCGCATTGTCACCGTAA

Protein sequence

MAAASSPTSFNSSSSSSSFSLPTHHHSPSHFTPIQECDREEREDSIPAENRTQITAAATGGCTDRGTSPKYHPTPLNRSKNGKSQTVKKRSESESDSVSSSDGPVSCNRCRPHAREKISVVPLDNNNGVNKQTYFSMASPNGIFKSLISSLTRKSPKSINESSALTAREEQWRTAVTELSQKLVQATRKRDEAIMEASRLKYAMAELEKKLDKLETYCHSLKSGIEECSGNSPSPCQIGKYNQIQSFQQRNQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYDIKISFSKNPRSMLFYLEALLNQAFFEDFESIGFQKNASTQVLNPIERCEANFGCFNLLHELTWEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFSASKSVWLLHLLANSVHPNLPIFRVDKEADFDSVYMEDMGGDKARKLIPSLVRIMIAPGFYVYGSVVKCKVLCRYNAAASAVTAAALSP
Homology
BLAST of HG10009250 vs. NCBI nr
Match: XP_038875926.1 (IRK-interacting protein [Benincasa hispida])

HSP 1 Score: 876.3 bits (2263), Expect = 1.2e-250
Identity = 462/504 (91.67%), Postives = 469/504 (93.06%), Query Frame = 0

Query: 1   MAAASSPTSFNSSSSSSSFSLPTHHHSPSHFTPIQECDREEREDSIPAENRTQITAAATG 60
           MAAA SPTSFNSS +      PT HH  S FTPIQE +RE RED+IP +NRTQ TAA TG
Sbjct: 1   MAAAPSPTSFNSSPA------PTLHHCSSLFTPIQESEREGREDAIPVDNRTQNTAAGTG 60

Query: 61  GCTDRGTSPKYHPTPLNRSKNGKSQTVKKRSESESDSVSSSDGPVSCNRCRPHAREKISV 120
           GCTDRGTSPK+H TPLNR+KNGKS TVKKRSESESDSVSSSDGPVSCNRCRPH REKISV
Sbjct: 61  GCTDRGTSPKHHSTPLNRTKNGKSHTVKKRSESESDSVSSSDGPVSCNRCRPHVREKISV 120

Query: 121 VPLDNNNGVNKQTYFSMASPNGIFKSLISSLTRKSPKSINESSALTAREEQWRTAVTELS 180
           VPLDNNNGVNKQTYFSMASPNGIFKSLISSLTRKSPKSINESSALTAREEQWR AVTELS
Sbjct: 121 VPLDNNNGVNKQTYFSMASPNGIFKSLISSLTRKSPKSINESSALTAREEQWRAAVTELS 180

Query: 181 QKLVQATRKRDEAIMEASRLKYAMAELEKKLDKLETYCHSLKSGIEECSGNSPSPCQIGK 240
           QKLVQATRKRDEAIMEASRLKYAMAELEKKLDKLETYCH LKSGIEECSGN  SPCQIG 
Sbjct: 181 QKLVQATRKRDEAIMEASRLKYAMAELEKKLDKLETYCHGLKSGIEECSGN--SPCQIGM 240

Query: 241 YNQIQSFQQRNQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYD 300
           YNQIQSFQQRNQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYD
Sbjct: 241 YNQIQSFQQRNQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYD 300

Query: 301 IKISFSKNPRSMLFYLEALLNQAFFEDFESIGFQKNASTQVLNPIERCEANFGCFNLLHE 360
           +KISFSKNPRSMLFYLEALLNQAFFEDFESIGFQKNASTQVLNPIERCE NF CFN LHE
Sbjct: 301 VKISFSKNPRSMLFYLEALLNQAFFEDFESIGFQKNASTQVLNPIERCETNFECFNFLHE 360

Query: 361 LTWEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFSASKSVWLLHLL 420
           LTWEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFSASKSVWLLHLL
Sbjct: 361 LTWEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFSASKSVWLLHLL 420

Query: 421 ANSVHPNLPIFRVDKEADFDSVYMEDMGGDKARKLIPSLVRIMIAPGFYVYGSVVKCKVL 480
           ANSVHPNLPIFRVDKEADFDSVYMEDMGGDKARKLIPSLVRIMIAPGFYVYGSVVKCKVL
Sbjct: 421 ANSVHPNLPIFRVDKEADFDSVYMEDMGGDKARKLIPSLVRIMIAPGFYVYGSVVKCKVL 480

Query: 481 CRYN-------AAASAVTAAALSP 498
           CRYN       AAASAVTAAALSP
Sbjct: 481 CRYNAATAAATAAASAVTAAALSP 496

BLAST of HG10009250 vs. NCBI nr
Match: XP_004147608.3 (IRK-interacting protein [Cucumis sativus] >KGN49712.2 hypothetical protein Csa_000067 [Cucumis sativus])

HSP 1 Score: 851.3 bits (2198), Expect = 4.2e-243
Identity = 452/506 (89.33%), Postives = 460/506 (90.91%), Query Frame = 0

Query: 3   AASSPTSFNSSSSSSSFSLPTHHHSPSHFTPIQECDREEREDSIPAENRTQITAAATGGC 62
           AA SPTSF SS               SHFTPIQECDREERED+         +A A GGC
Sbjct: 2   AAPSPTSFISS---------------SHFTPIQECDREEREDT---------SATAAGGC 61

Query: 63  TDRGTSPKYHPTPLNRSKNGKSQTVKKRSESESDSVSSSDGPVSCNRCRPHAREKISVVP 122
           TDRGTSPK++PTPLNR+KNGKSQT+KKRSESESDSVSSSDGPVSCNRCRPHAREKISVVP
Sbjct: 62  TDRGTSPKHYPTPLNRNKNGKSQTIKKRSESESDSVSSSDGPVSCNRCRPHAREKISVVP 121

Query: 123 LDNNNGVNKQTYFSMASPNGIFKSLISSLTRKSPKSINESSALTAREEQWRTAVTELSQK 182
           LDNNNGVNKQTYFSMASPNGIFKSLISSLTRKSPKSINESSALTAREEQWR AVTELSQK
Sbjct: 122 LDNNNGVNKQTYFSMASPNGIFKSLISSLTRKSPKSINESSALTAREEQWRAAVTELSQK 181

Query: 183 LVQATRKRDEAIMEASRLKYAMAELEKKLDKLETYCHSLKSGIEECSGNSPSPCQIGKYN 242
           LVQATRKRDEA+MEASRLKYAMAELEKKLDKLETYCHSLKSGIEECSGN  SPCQIGKYN
Sbjct: 182 LVQATRKRDEAVMEASRLKYAMAELEKKLDKLETYCHSLKSGIEECSGN--SPCQIGKYN 241

Query: 243 QIQSFQQRNQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYDIK 302
           QIQSFQQ NQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYDIK
Sbjct: 242 QIQSFQQSNQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYDIK 301

Query: 303 ISFSKNPRSMLFYLEALLNQAFFEDFESIGFQKNASTQVLNPIERCEANFGCFNLLHELT 362
            SFSKNPRSMLFYLEALLNQAFFEDFESIGFQKNASTQVLNPIERCEANF CFN LHELT
Sbjct: 302 TSFSKNPRSMLFYLEALLNQAFFEDFESIGFQKNASTQVLNPIERCEANFECFNFLHELT 361

Query: 363 WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFSASKSVWLLHLLAN 422
           WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFSASKSVWLLHLLAN
Sbjct: 362 WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFSASKSVWLLHLLAN 421

Query: 423 SVHPNLPIFRVDKEADFDSVYMEDMGGDKARKLIPSLVRIMIAPGFYVYGSVVKCKVLCR 482
           SVHPNLPIFRV+KEADFDSVYMEDMGGDKARKLIPSLVRIMIAPGFYVYGSVVKCKVLCR
Sbjct: 422 SVHPNLPIFRVEKEADFDSVYMEDMGGDKARKLIPSLVRIMIAPGFYVYGSVVKCKVLCR 481

Query: 483 YNAA-----------ASAVTAAALSP 498
           YNAA           ASAVTAAALSP
Sbjct: 482 YNAATATATATATATASAVTAAALSP 481

BLAST of HG10009250 vs. NCBI nr
Match: XP_008437154.1 (PREDICTED: IRK-interacting protein [Cucumis melo] >KAA0042846.1 IRK-interacting protein [Cucumis melo var. makuwa])

HSP 1 Score: 844.7 bits (2181), Expect = 4.0e-241
Identity = 448/496 (90.32%), Postives = 456/496 (91.94%), Query Frame = 0

Query: 3   AASSPTSFNSSSSSSSFSLPTHHHSPSHFTPIQECDREEREDSIPAENRTQITAAATGGC 62
           AA SPTSF SS               SHFTPIQECDREERED          T+AA GGC
Sbjct: 2   AAPSPTSFISS---------------SHFTPIQECDREERED----------TSAAAGGC 61

Query: 63  TDRGTSPKYHPTPLNRSKNGKSQTVKKRSESESDSVSSSDGPVSCNRCRPHAREKISVVP 122
           TD GTSPK +PTPLNR+ NGKSQTVKKRSESESDSVSSSDGPVSCNRCRPHAREKISVVP
Sbjct: 62  TDLGTSPKLYPTPLNRNMNGKSQTVKKRSESESDSVSSSDGPVSCNRCRPHAREKISVVP 121

Query: 123 LDNNNGVNKQTYFSMASPNGIFKSLISSLTRKSPKSINESSALTAREEQWRTAVTELSQK 182
           LDN+NGVNKQTYFSMASPNGIFKSLISSLTRKSPKSINESSALTAREEQWR AVTELSQK
Sbjct: 122 LDNSNGVNKQTYFSMASPNGIFKSLISSLTRKSPKSINESSALTAREEQWRAAVTELSQK 181

Query: 183 LVQATRKRDEAIMEASRLKYAMAELEKKLDKLETYCHSLKSGIEECSGNSPSPCQIGKYN 242
           LVQATRKRDEA+MEASRLKYAMAELEKKLDKLETYCHSLKSGIEECSGN  SPCQIGKYN
Sbjct: 182 LVQATRKRDEAVMEASRLKYAMAELEKKLDKLETYCHSLKSGIEECSGN--SPCQIGKYN 241

Query: 243 QIQSFQQRNQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYDIK 302
           QIQSFQQ NQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERIS+LLQPYDIK
Sbjct: 242 QIQSFQQSNQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISLLLQPYDIK 301

Query: 303 ISFSKNPRSMLFYLEALLNQAFFEDFESIGFQKNASTQVLNPIERCEANFGCFNLLHELT 362
           ISFSKNPRSMLFYLEALLNQAFFEDFESIGFQKNASTQVLNPIERCEANF CFN LHELT
Sbjct: 302 ISFSKNPRSMLFYLEALLNQAFFEDFESIGFQKNASTQVLNPIERCEANFECFNFLHELT 361

Query: 363 WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFSASKSVWLLHLLAN 422
           WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFSASKSVWLLHLLAN
Sbjct: 362 WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFSASKSVWLLHLLAN 421

Query: 423 SVHPNLPIFRVDKEADFDSVYMEDMGGDKARKLIPSLVRIMIAPGFYVYGSVVKCKVLCR 482
           SVHPNLPIFRV+KEADFDSVYMEDMGGDKARKLIPSLVRIMI+PGFYVYGSVVKCKVLCR
Sbjct: 422 SVHPNLPIFRVEKEADFDSVYMEDMGGDKARKLIPSLVRIMISPGFYVYGSVVKCKVLCR 470

Query: 483 YN-AAASAVTAAALSP 498
           YN AAASAVTA  LSP
Sbjct: 482 YNAAAASAVTAPVLSP 470

BLAST of HG10009250 vs. NCBI nr
Match: XP_022922117.1 (IRK-interacting protein-like [Cucurbita moschata])

HSP 1 Score: 839.3 bits (2167), Expect = 1.7e-239
Identity = 445/495 (89.90%), Postives = 460/495 (92.93%), Query Frame = 0

Query: 3   AASSPTSFNSSSSSSSFSLPTHHHSPSHFTPIQECDREEREDSIPAENRTQITAAATGGC 62
           A  SPTSF SS SSSS + PT HHS SHFTPIQEC+REERED   A+NRTQ TAA   GC
Sbjct: 28  APPSPTSFYSSPSSSS-AAPT-HHSRSHFTPIQECEREEREDEFAAQNRTQNTAAV--GC 87

Query: 63  TDRGTSPKYHPTPLNRSKNGKSQTVKKRSESESDSVSSSDGPVSCNRCRPHAREKISVVP 122
           TDRGTSP  H TPLNR      +TVKKRSESESDSVSSSDGPVSCNRCRPHAREKISVVP
Sbjct: 88  TDRGTSPNLHSTPLNR------ETVKKRSESESDSVSSSDGPVSCNRCRPHAREKISVVP 147

Query: 123 LDNNNGVNKQTYFSMASPNGIFKSLISSLTRKSPKSINESSALTAREEQWRTAVTELSQK 182
           LDNNNGVNKQTYFSMASPNGIFKSLISSLTR+SPKSINESSALTAREEQWRTAVTELSQK
Sbjct: 148 LDNNNGVNKQTYFSMASPNGIFKSLISSLTRRSPKSINESSALTAREEQWRTAVTELSQK 207

Query: 183 LVQATRKRDEAIMEASRLKYAMAELEKKLDKLETYCHSLKSGIEECSGNSPSPCQIGKYN 242
           LVQATRKRDEAIMEASRLKYAMAELEKKL+KLETYCHSLKSGIEECSGN  SPCQIG Y+
Sbjct: 208 LVQATRKRDEAIMEASRLKYAMAELEKKLNKLETYCHSLKSGIEECSGN--SPCQIGNYH 267

Query: 243 QIQSFQQRNQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYDIK 302
           QIQ  QQ NQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYDIK
Sbjct: 268 QIQDLQQMNQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYDIK 327

Query: 303 ISFSKNPRSMLFYLEALLNQAFFEDFESIGFQKNASTQVLNPIERCEANFGCFNLLHELT 362
           ISFSKNPRS+LFYLEALLNQAFFEDFES+GFQKNASTQ+LNPIERC+ANFGCFN LHELT
Sbjct: 328 ISFSKNPRSLLFYLEALLNQAFFEDFESVGFQKNASTQILNPIERCDANFGCFNFLHELT 387

Query: 363 WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFSASKSVWLLHLLAN 422
           WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFF ASKSVWLLHLLAN
Sbjct: 388 WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFGASKSVWLLHLLAN 447

Query: 423 SVHPNLPIFRVDKEADFDSVYMEDMGGDKARKLIPSLVRIMIAPGFYVYGSVVKCKVLCR 482
           SVHPNLPIFRV+KEADFDSVYMEDM G+KARKLIPS+VRIMIAPGFYVYGSVVKCKVLCR
Sbjct: 448 SVHPNLPIFRVEKEADFDSVYMEDMSGEKARKLIPSVVRIMIAPGFYVYGSVVKCKVLCR 507

Query: 483 YNAAASAVTAAALSP 498
           YNAAA+  TAAALSP
Sbjct: 508 YNAAAA--TAAALSP 508

BLAST of HG10009250 vs. NCBI nr
Match: KAG6579443.1 (IRK-interacting protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016916.1 IRK-interacting protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 839.0 bits (2166), Expect = 2.2e-239
Identity = 445/495 (89.90%), Postives = 460/495 (92.93%), Query Frame = 0

Query: 3   AASSPTSFNSSSSSSSFSLPTHHHSPSHFTPIQECDREEREDSIPAENRTQITAAATGGC 62
           A  SPTSF SS SSSS + PT HHS SHFTPIQEC+REERED   A+NRTQ TAA   GC
Sbjct: 29  APPSPTSFYSSPSSSS-AAPT-HHSRSHFTPIQECEREEREDEFAAQNRTQNTAAV--GC 88

Query: 63  TDRGTSPKYHPTPLNRSKNGKSQTVKKRSESESDSVSSSDGPVSCNRCRPHAREKISVVP 122
           TDRGTSP  H TPLNR      +TVKKRSESESDSVSSSDGPVSCNRCRPHAREKISVVP
Sbjct: 89  TDRGTSPNLHSTPLNR------ETVKKRSESESDSVSSSDGPVSCNRCRPHAREKISVVP 148

Query: 123 LDNNNGVNKQTYFSMASPNGIFKSLISSLTRKSPKSINESSALTAREEQWRTAVTELSQK 182
           LDNNNGVNKQTYFSMASPNGIFKSLISSLTR+SPKSINESSALTAREEQWRTAVTELSQK
Sbjct: 149 LDNNNGVNKQTYFSMASPNGIFKSLISSLTRRSPKSINESSALTAREEQWRTAVTELSQK 208

Query: 183 LVQATRKRDEAIMEASRLKYAMAELEKKLDKLETYCHSLKSGIEECSGNSPSPCQIGKYN 242
           LVQATRKRDEAIMEASRLKYAMAELEKKL+KLETYCHSLKSGIEECSGN  SPCQIG Y+
Sbjct: 209 LVQATRKRDEAIMEASRLKYAMAELEKKLNKLETYCHSLKSGIEECSGN--SPCQIGHYH 268

Query: 243 QIQSFQQRNQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYDIK 302
           QIQ  QQ NQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYDIK
Sbjct: 269 QIQDLQQMNQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYDIK 328

Query: 303 ISFSKNPRSMLFYLEALLNQAFFEDFESIGFQKNASTQVLNPIERCEANFGCFNLLHELT 362
           ISFSKNPRS+LFYLEALLNQAFFEDFES+GFQKNASTQ+LNPIERC+ANFGCFN LHELT
Sbjct: 329 ISFSKNPRSLLFYLEALLNQAFFEDFESVGFQKNASTQILNPIERCDANFGCFNFLHELT 388

Query: 363 WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFSASKSVWLLHLLAN 422
           WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFF ASKSVWLLHLLAN
Sbjct: 389 WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFGASKSVWLLHLLAN 448

Query: 423 SVHPNLPIFRVDKEADFDSVYMEDMGGDKARKLIPSLVRIMIAPGFYVYGSVVKCKVLCR 482
           SVHPNLPIFRV+KEADFDSVYMEDM G+KARKLIPS+VRIMIAPGFYVYGSVVKCKVLCR
Sbjct: 449 SVHPNLPIFRVEKEADFDSVYMEDMSGEKARKLIPSVVRIMIAPGFYVYGSVVKCKVLCR 508

Query: 483 YNAAASAVTAAALSP 498
           YNAAA+  TAAALSP
Sbjct: 509 YNAAAA--TAAALSP 509

BLAST of HG10009250 vs. ExPASy Swiss-Prot
Match: Q9LXU9 (IRK-interacting protein OS=Arabidopsis thaliana OX=3702 GN=IRKI PE=1 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 7.4e-65
Identity = 137/347 (39.48%), Postives = 216/347 (62.25%), Query Frame = 0

Query: 153 RKSPKSINESSALTAREEQWR-------TAVTELSQKLVQATRKRDEAIMEASRLKYAMA 212
           ++   SI  S ++T + E+         + V +L ++L++A R RD A+ + S +K ++ 
Sbjct: 218 KQKSNSIFNSPSITEKSEEVSEVLKDSGSGVEKLKRELMEANRSRDAALTQVSEMKSSLG 277

Query: 213 ELEKKLDKLETYCHSLKSGIEEC----SGNSPSPCQIGKYNQIQSFQQRNQKQVIEHFLV 272
           EL +KL  LE+YC +LK  + E     S  +      GK N   S    +++ ++E FL 
Sbjct: 278 ELSEKLQYLESYCDNLKKALREATEVVSQENSGGRSSGKKN---SEMPVSEEVMVEGFLQ 337

Query: 273 SVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYDIKISFSKNPRSMLFYLEALLN 332
            VSE+R SI+   ++L  ++    + +   I+ LLQP+++  + SK  + + ++LEA+++
Sbjct: 338 IVSEARLSIKQFLKTLVSEIDEEDSTLIGNINTLLQPHNLSFT-SKYSKIIQYHLEAIIS 397

Query: 333 QAFFEDFESIGFQKNASTQVLNPIERCEANFGCFNLLHELTWEEVLSKGTKHFSEDFSRF 392
           Q+ ++DFE+  FQKN   ++L+P +  +ANF  F  L  L+W EVL KGTK++S++FSRF
Sbjct: 398 QSVYQDFENCVFQKNGKPKLLDPEQDRQANFSSFASLRNLSWNEVLKKGTKYYSDEFSRF 457

Query: 393 CDRKMSEIVAMLGWNRAWPEPLLQAFFSASKSVWLLHLLANSVHPNLPIFRVDKEADFDS 452
           CD KMS I+  L W R W E +LQAFF A+K VWLLHLLA S +P L I RV++  +F+S
Sbjct: 458 CDEKMSLIITTLNWTRPWSEQMLQAFFVAAKCVWLLHLLAFSFNPALGILRVEENREFES 517

Query: 453 VYMEDMGGDKARKLI---PSLVRIMIAPGFYVYGSVVKCKVLCRYNA 486
            +MEDMG D+ R  +   P+ V++M+ PGFYV   V++CKVLCRY +
Sbjct: 518 SFMEDMGADRQRSALSRGPARVKVMVMPGFYVLDRVLRCKVLCRYKS 560

BLAST of HG10009250 vs. ExPASy Swiss-Prot
Match: F4KGE8 (Protein GRAVITROPIC IN THE LIGHT 1 OS=Arabidopsis thaliana OX=3702 GN=GIL1 PE=1 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 6.0e-06
Identity = 52/188 (27.66%), Postives = 85/188 (45.21%), Query Frame = 0

Query: 314 FYLEALLNQAFFEDFESIGFQKNAS-TQVLNPIERCEANFGCFNLLHELTWEEVLS-KGT 373
           F LE+ + +  F+ F+   F  + S + ++NP +     F  F  +  +   E+L    T
Sbjct: 370 FALESYICRKIFQGFDHETFYMDGSLSSLINPDQYRRDCFAQFKDMKAMDPMELLGILPT 429

Query: 374 KHFSEDFSRFCDRKMSEIV------AMLGWNR------AWPEPLLQ---AFFSASKSVWL 433
            H    F +FC +K   I+      ++ G +       A   P  Q    F   +K+VWL
Sbjct: 430 CH----FGKFCSKKYLSIIHQKMEESLFGDSEQRELVVAGNHPRSQFYGEFLGLAKAVWL 489

Query: 434 LHLLANSVHPNLPIFRVDKEADFDSVYMED-MGGDKARKLIPSLVRIMIAPGFYV----Y 480
           LHLLA S+ P+   F  ++ A+F S YME  +     R     +V   + PGF +     
Sbjct: 490 LHLLAFSLDPSPSHFEANRGAEFHSQYMESVVRFSDGRVPAGQVVGFPVCPGFKLSHQGK 549

BLAST of HG10009250 vs. ExPASy TrEMBL
Match: A0A0A0KMS3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G158500 PE=4 SV=1)

HSP 1 Score: 851.7 bits (2199), Expect = 1.6e-243
Identity = 451/502 (89.84%), Postives = 460/502 (91.63%), Query Frame = 0

Query: 3   AASSPTSFNSSSSSSSFSLPTHHHSPSHFTPIQECDREEREDSIPAENRTQITAAATGGC 62
           AA SPTSF SS               SHFTPIQECDREERED+         +A A GGC
Sbjct: 2   AAPSPTSFISS---------------SHFTPIQECDREEREDT---------SATAAGGC 61

Query: 63  TDRGTSPKYHPTPLNRSKNGKSQTVKKRSESESDSVSSSDGPVSCNRCRPHAREKISVVP 122
           TDRGTSPK++PTPLNR+KNGKSQT+KKRSESESDSVSSSDGPVSCNRCRPHAREKISVVP
Sbjct: 62  TDRGTSPKHYPTPLNRNKNGKSQTIKKRSESESDSVSSSDGPVSCNRCRPHAREKISVVP 121

Query: 123 LDNNNGVNKQTYFSMASPNGIFKSLISSLTRKSPKSINESSALTAREEQWRTAVTELSQK 182
           LDNNNGVNKQTYFSMASPNGIFKSLISSLTRKSPKSINESSALTAREEQWR AVTELSQK
Sbjct: 122 LDNNNGVNKQTYFSMASPNGIFKSLISSLTRKSPKSINESSALTAREEQWRAAVTELSQK 181

Query: 183 LVQATRKRDEAIMEASRLKYAMAELEKKLDKLETYCHSLKSGIEECSGNSPSPCQIGKYN 242
           LVQATRKRDEA+MEASRLKYAMAELEKKLDKLETYCHSLKSGIEECSGN  SPCQIGKYN
Sbjct: 182 LVQATRKRDEAVMEASRLKYAMAELEKKLDKLETYCHSLKSGIEECSGN--SPCQIGKYN 241

Query: 243 QIQSFQQRNQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYDIK 302
           QIQSFQQ NQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYDIK
Sbjct: 242 QIQSFQQSNQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYDIK 301

Query: 303 ISFSKNPRSMLFYLEALLNQAFFEDFESIGFQKNASTQVLNPIERCEANFGCFNLLHELT 362
            SFSKNPRSMLFYLEALLNQAFFEDFESIGFQKNASTQVLNPIERCEANF CFN LHELT
Sbjct: 302 TSFSKNPRSMLFYLEALLNQAFFEDFESIGFQKNASTQVLNPIERCEANFECFNFLHELT 361

Query: 363 WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFSASKSVWLLHLLAN 422
           WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFSASKSVWLLHLLAN
Sbjct: 362 WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFSASKSVWLLHLLAN 421

Query: 423 SVHPNLPIFRVDKEADFDSVYMEDMGGDKARKLIPSLVRIMIAPGFYVYGSVVKCKVLCR 482
           SVHPNLPIFRV+KEADFDSVYMEDMGGDKARKLIPSLVRIMIAPGFYVYGSVVKCKVLCR
Sbjct: 422 SVHPNLPIFRVEKEADFDSVYMEDMGGDKARKLIPSLVRIMIAPGFYVYGSVVKCKVLCR 477

Query: 483 YNAA-------ASAVTAAALSP 498
           YNAA       A+AVTAAALSP
Sbjct: 482 YNAATATATATATAVTAAALSP 477

BLAST of HG10009250 vs. ExPASy TrEMBL
Match: A0A5A7TLI4 (IRK-interacting protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003570 PE=4 SV=1)

HSP 1 Score: 844.7 bits (2181), Expect = 1.9e-241
Identity = 448/496 (90.32%), Postives = 456/496 (91.94%), Query Frame = 0

Query: 3   AASSPTSFNSSSSSSSFSLPTHHHSPSHFTPIQECDREEREDSIPAENRTQITAAATGGC 62
           AA SPTSF SS               SHFTPIQECDREERED          T+AA GGC
Sbjct: 2   AAPSPTSFISS---------------SHFTPIQECDREERED----------TSAAAGGC 61

Query: 63  TDRGTSPKYHPTPLNRSKNGKSQTVKKRSESESDSVSSSDGPVSCNRCRPHAREKISVVP 122
           TD GTSPK +PTPLNR+ NGKSQTVKKRSESESDSVSSSDGPVSCNRCRPHAREKISVVP
Sbjct: 62  TDLGTSPKLYPTPLNRNMNGKSQTVKKRSESESDSVSSSDGPVSCNRCRPHAREKISVVP 121

Query: 123 LDNNNGVNKQTYFSMASPNGIFKSLISSLTRKSPKSINESSALTAREEQWRTAVTELSQK 182
           LDN+NGVNKQTYFSMASPNGIFKSLISSLTRKSPKSINESSALTAREEQWR AVTELSQK
Sbjct: 122 LDNSNGVNKQTYFSMASPNGIFKSLISSLTRKSPKSINESSALTAREEQWRAAVTELSQK 181

Query: 183 LVQATRKRDEAIMEASRLKYAMAELEKKLDKLETYCHSLKSGIEECSGNSPSPCQIGKYN 242
           LVQATRKRDEA+MEASRLKYAMAELEKKLDKLETYCHSLKSGIEECSGN  SPCQIGKYN
Sbjct: 182 LVQATRKRDEAVMEASRLKYAMAELEKKLDKLETYCHSLKSGIEECSGN--SPCQIGKYN 241

Query: 243 QIQSFQQRNQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYDIK 302
           QIQSFQQ NQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERIS+LLQPYDIK
Sbjct: 242 QIQSFQQSNQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISLLLQPYDIK 301

Query: 303 ISFSKNPRSMLFYLEALLNQAFFEDFESIGFQKNASTQVLNPIERCEANFGCFNLLHELT 362
           ISFSKNPRSMLFYLEALLNQAFFEDFESIGFQKNASTQVLNPIERCEANF CFN LHELT
Sbjct: 302 ISFSKNPRSMLFYLEALLNQAFFEDFESIGFQKNASTQVLNPIERCEANFECFNFLHELT 361

Query: 363 WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFSASKSVWLLHLLAN 422
           WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFSASKSVWLLHLLAN
Sbjct: 362 WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFSASKSVWLLHLLAN 421

Query: 423 SVHPNLPIFRVDKEADFDSVYMEDMGGDKARKLIPSLVRIMIAPGFYVYGSVVKCKVLCR 482
           SVHPNLPIFRV+KEADFDSVYMEDMGGDKARKLIPSLVRIMI+PGFYVYGSVVKCKVLCR
Sbjct: 422 SVHPNLPIFRVEKEADFDSVYMEDMGGDKARKLIPSLVRIMISPGFYVYGSVVKCKVLCR 470

Query: 483 YN-AAASAVTAAALSP 498
           YN AAASAVTA  LSP
Sbjct: 482 YNAAAASAVTAPVLSP 470

BLAST of HG10009250 vs. ExPASy TrEMBL
Match: A0A1S3ATV6 (IRK-interacting protein OS=Cucumis melo OX=3656 GN=LOC103482662 PE=4 SV=1)

HSP 1 Score: 844.7 bits (2181), Expect = 1.9e-241
Identity = 448/496 (90.32%), Postives = 456/496 (91.94%), Query Frame = 0

Query: 3   AASSPTSFNSSSSSSSFSLPTHHHSPSHFTPIQECDREEREDSIPAENRTQITAAATGGC 62
           AA SPTSF SS               SHFTPIQECDREERED          T+AA GGC
Sbjct: 2   AAPSPTSFISS---------------SHFTPIQECDREERED----------TSAAAGGC 61

Query: 63  TDRGTSPKYHPTPLNRSKNGKSQTVKKRSESESDSVSSSDGPVSCNRCRPHAREKISVVP 122
           TD GTSPK +PTPLNR+ NGKSQTVKKRSESESDSVSSSDGPVSCNRCRPHAREKISVVP
Sbjct: 62  TDLGTSPKLYPTPLNRNMNGKSQTVKKRSESESDSVSSSDGPVSCNRCRPHAREKISVVP 121

Query: 123 LDNNNGVNKQTYFSMASPNGIFKSLISSLTRKSPKSINESSALTAREEQWRTAVTELSQK 182
           LDN+NGVNKQTYFSMASPNGIFKSLISSLTRKSPKSINESSALTAREEQWR AVTELSQK
Sbjct: 122 LDNSNGVNKQTYFSMASPNGIFKSLISSLTRKSPKSINESSALTAREEQWRAAVTELSQK 181

Query: 183 LVQATRKRDEAIMEASRLKYAMAELEKKLDKLETYCHSLKSGIEECSGNSPSPCQIGKYN 242
           LVQATRKRDEA+MEASRLKYAMAELEKKLDKLETYCHSLKSGIEECSGN  SPCQIGKYN
Sbjct: 182 LVQATRKRDEAVMEASRLKYAMAELEKKLDKLETYCHSLKSGIEECSGN--SPCQIGKYN 241

Query: 243 QIQSFQQRNQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYDIK 302
           QIQSFQQ NQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERIS+LLQPYDIK
Sbjct: 242 QIQSFQQSNQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISLLLQPYDIK 301

Query: 303 ISFSKNPRSMLFYLEALLNQAFFEDFESIGFQKNASTQVLNPIERCEANFGCFNLLHELT 362
           ISFSKNPRSMLFYLEALLNQAFFEDFESIGFQKNASTQVLNPIERCEANF CFN LHELT
Sbjct: 302 ISFSKNPRSMLFYLEALLNQAFFEDFESIGFQKNASTQVLNPIERCEANFECFNFLHELT 361

Query: 363 WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFSASKSVWLLHLLAN 422
           WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFSASKSVWLLHLLAN
Sbjct: 362 WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFSASKSVWLLHLLAN 421

Query: 423 SVHPNLPIFRVDKEADFDSVYMEDMGGDKARKLIPSLVRIMIAPGFYVYGSVVKCKVLCR 482
           SVHPNLPIFRV+KEADFDSVYMEDMGGDKARKLIPSLVRIMI+PGFYVYGSVVKCKVLCR
Sbjct: 422 SVHPNLPIFRVEKEADFDSVYMEDMGGDKARKLIPSLVRIMISPGFYVYGSVVKCKVLCR 470

Query: 483 YN-AAASAVTAAALSP 498
           YN AAASAVTA  LSP
Sbjct: 482 YNAAAASAVTAPVLSP 470

BLAST of HG10009250 vs. ExPASy TrEMBL
Match: A0A6J1E2B7 (IRK-interacting protein-like OS=Cucurbita moschata OX=3662 GN=LOC111430185 PE=4 SV=1)

HSP 1 Score: 839.3 bits (2167), Expect = 8.1e-240
Identity = 445/495 (89.90%), Postives = 460/495 (92.93%), Query Frame = 0

Query: 3   AASSPTSFNSSSSSSSFSLPTHHHSPSHFTPIQECDREEREDSIPAENRTQITAAATGGC 62
           A  SPTSF SS SSSS + PT HHS SHFTPIQEC+REERED   A+NRTQ TAA   GC
Sbjct: 28  APPSPTSFYSSPSSSS-AAPT-HHSRSHFTPIQECEREEREDEFAAQNRTQNTAAV--GC 87

Query: 63  TDRGTSPKYHPTPLNRSKNGKSQTVKKRSESESDSVSSSDGPVSCNRCRPHAREKISVVP 122
           TDRGTSP  H TPLNR      +TVKKRSESESDSVSSSDGPVSCNRCRPHAREKISVVP
Sbjct: 88  TDRGTSPNLHSTPLNR------ETVKKRSESESDSVSSSDGPVSCNRCRPHAREKISVVP 147

Query: 123 LDNNNGVNKQTYFSMASPNGIFKSLISSLTRKSPKSINESSALTAREEQWRTAVTELSQK 182
           LDNNNGVNKQTYFSMASPNGIFKSLISSLTR+SPKSINESSALTAREEQWRTAVTELSQK
Sbjct: 148 LDNNNGVNKQTYFSMASPNGIFKSLISSLTRRSPKSINESSALTAREEQWRTAVTELSQK 207

Query: 183 LVQATRKRDEAIMEASRLKYAMAELEKKLDKLETYCHSLKSGIEECSGNSPSPCQIGKYN 242
           LVQATRKRDEAIMEASRLKYAMAELEKKL+KLETYCHSLKSGIEECSGN  SPCQIG Y+
Sbjct: 208 LVQATRKRDEAIMEASRLKYAMAELEKKLNKLETYCHSLKSGIEECSGN--SPCQIGNYH 267

Query: 243 QIQSFQQRNQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYDIK 302
           QIQ  QQ NQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYDIK
Sbjct: 268 QIQDLQQMNQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYDIK 327

Query: 303 ISFSKNPRSMLFYLEALLNQAFFEDFESIGFQKNASTQVLNPIERCEANFGCFNLLHELT 362
           ISFSKNPRS+LFYLEALLNQAFFEDFES+GFQKNASTQ+LNPIERC+ANFGCFN LHELT
Sbjct: 328 ISFSKNPRSLLFYLEALLNQAFFEDFESVGFQKNASTQILNPIERCDANFGCFNFLHELT 387

Query: 363 WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFSASKSVWLLHLLAN 422
           WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFF ASKSVWLLHLLAN
Sbjct: 388 WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFGASKSVWLLHLLAN 447

Query: 423 SVHPNLPIFRVDKEADFDSVYMEDMGGDKARKLIPSLVRIMIAPGFYVYGSVVKCKVLCR 482
           SVHPNLPIFRV+KEADFDSVYMEDM G+KARKLIPS+VRIMIAPGFYVYGSVVKCKVLCR
Sbjct: 448 SVHPNLPIFRVEKEADFDSVYMEDMSGEKARKLIPSVVRIMIAPGFYVYGSVVKCKVLCR 507

Query: 483 YNAAASAVTAAALSP 498
           YNAAA+  TAAALSP
Sbjct: 508 YNAAAA--TAAALSP 508

BLAST of HG10009250 vs. ExPASy TrEMBL
Match: A0A6J1I269 (IRK-interacting protein-like OS=Cucurbita maxima OX=3661 GN=LOC111469159 PE=4 SV=1)

HSP 1 Score: 833.2 bits (2151), Expect = 5.8e-238
Identity = 441/495 (89.09%), Postives = 457/495 (92.32%), Query Frame = 0

Query: 3   AASSPTSFNSSSSSSSFSLPTHHHSPSHFTPIQECDREEREDSIPAENRTQITAAATGGC 62
           A  SPTSF+SS SSSS + PTHH  P HFTPIQEC+REERED   A NRTQ TAA   GC
Sbjct: 21  APPSPTSFHSSPSSSS-AAPTHHSRP-HFTPIQECEREEREDEFAAHNRTQNTAAV--GC 80

Query: 63  TDRGTSPKYHPTPLNRSKNGKSQTVKKRSESESDSVSSSDGPVSCNRCRPHAREKISVVP 122
           TDRGTSP  H TPLNR      +TVKKRSESESDSVSSSDGPVSCNRCRPHAREK SVVP
Sbjct: 81  TDRGTSPNPHSTPLNR------ETVKKRSESESDSVSSSDGPVSCNRCRPHAREKFSVVP 140

Query: 123 LDNNNGVNKQTYFSMASPNGIFKSLISSLTRKSPKSINESSALTAREEQWRTAVTELSQK 182
           LDNNNGVNKQTYFSMASPNGIFKSLISSLTR+SPKSINESSALTAREEQWRTAVTELSQK
Sbjct: 141 LDNNNGVNKQTYFSMASPNGIFKSLISSLTRRSPKSINESSALTAREEQWRTAVTELSQK 200

Query: 183 LVQATRKRDEAIMEASRLKYAMAELEKKLDKLETYCHSLKSGIEECSGNSPSPCQIGKYN 242
           LVQATRKRDEAIMEASRLKYAMAELEKKL+KLETYCHSLKSGIEECSGN  SPCQIG Y+
Sbjct: 201 LVQATRKRDEAIMEASRLKYAMAELEKKLNKLETYCHSLKSGIEECSGN--SPCQIGNYH 260

Query: 243 QIQSFQQRNQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYDIK 302
           QIQ  QQ NQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPY+IK
Sbjct: 261 QIQDLQQMNQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYNIK 320

Query: 303 ISFSKNPRSMLFYLEALLNQAFFEDFESIGFQKNASTQVLNPIERCEANFGCFNLLHELT 362
           ISFSKNPRS+LFYLEALLNQA FEDFES+GFQKNASTQ+LNPIERC+ANFGCFN LHELT
Sbjct: 321 ISFSKNPRSLLFYLEALLNQALFEDFESVGFQKNASTQILNPIERCDANFGCFNFLHELT 380

Query: 363 WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFSASKSVWLLHLLAN 422
           WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFF ASKSVWLLHLLAN
Sbjct: 381 WEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLLQAFFGASKSVWLLHLLAN 440

Query: 423 SVHPNLPIFRVDKEADFDSVYMEDMGGDKARKLIPSLVRIMIAPGFYVYGSVVKCKVLCR 482
           SVHPNLPIFRV+KEADFDSVYMEDM G+KARKLIPS+VRIMIAPGFYVYGSVVKCKVLCR
Sbjct: 441 SVHPNLPIFRVEKEADFDSVYMEDMSGEKARKLIPSVVRIMIAPGFYVYGSVVKCKVLCR 500

Query: 483 YNAAASAVTAAALSP 498
           YNAAA+  TAAALSP
Sbjct: 501 YNAAAA--TAAALSP 501

BLAST of HG10009250 vs. TAIR 10
Match: AT1G12330.1 (unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 12 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G12900.1); Has 249 Blast hits to 249 proteins in 27 species: Archae - 0; Bacteria - 0; Metazoa - 7; Fungi - 14; Plants - 217; Viruses - 0; Other Eukaryotes - 11 (source: NCBI BLink). )

HSP 1 Score: 491.1 bits (1263), Expect = 1.0e-138
Identity = 274/498 (55.02%), Postives = 361/498 (72.49%), Query Frame = 0

Query: 11  NSSSSSSSFSLPTHHHSPSHFTPIQECDREEREDSIPAENRTQITAAATGGC-------- 70
           +SSSS S   +P+ H S  HFTPI EC+ ++ ++      + + T ++ GG         
Sbjct: 4   SSSSSPSKSPVPSLHQS-LHFTPILECEEDDLQEE---RYKNRATPSSDGGSSATPNHQH 63

Query: 71  ---TDRGTSPKYHPTPLNRSKNGKSQTVKKRSESESDSVSSSDGPVSCNRCRPH--AREK 130
              T+   + ++  TPL+   NGK QT K+  +++ D      G VSCN+CRPH   R+K
Sbjct: 64  HRRTNSNNNHQHTLTPLHH--NGKPQTRKRHDDNDED----DGGAVSCNKCRPHHSHRDK 123

Query: 131 ISVVPLDNNNGVNKQTYFSMASPNGIFKSLISSLTRKSPK-----------SINESSALT 190
            SVVPL+++N  +      ++SPN I KS+  SLTR+SPK           S + S+A  
Sbjct: 124 FSVVPLESHNNPS-----FISSPNLIIKSIFQSLTRRSPKPSSATAALPPRSSSSSAADA 183

Query: 191 AREEQWRTAVTELSQKLVQATRKRDEAIMEASRLKYAMAELEKKLDKLETYCHSLKSGIE 250
           +REEQWR AV ELS KL+QAT+K+++A++EASRLK +MAELEKKL+KLE YCH+LKSG++
Sbjct: 184 SREEQWRLAVAELSHKLIQATKKKEDAVIEASRLKSSMAELEKKLNKLEIYCHNLKSGLD 243

Query: 251 ECSGNSPS-PCQIGKYNQIQSFQQRNQKQVIEHFLVSVSESRSSIRLLSRSLTLQLRHVG 310
           ECS    S P +   +N           ++I+ FLVSVSESRSSIR LSRSL  QLR VG
Sbjct: 244 ECSNKKQSVPIRKDGFND----------RIIQQFLVSVSESRSSIRALSRSLASQLRTVG 303

Query: 311 AKVYERISVLLQPYDIKI-SFSKNPRSMLFYLEALLNQAFFEDFESIGFQKNASTQVLNP 370
            KVYER+S+LLQP+D+KI SF+KNP+S++FYLEA+L++AFFEDFE+ GFQKN ST++LNP
Sbjct: 304 GKVYERLSLLLQPFDVKINSFAKNPKSLIFYLEAILSRAFFEDFEAPGFQKNGSTRILNP 363

Query: 371 IERCEANFGCFNLLHELTWEEVLSKGTKHFSEDFSRFCDRKMSEIVAMLGWNRAWPEPLL 430
           I+RCE+N+  FN+L ELTW+EVLS+GTKHFSE+FSRFCDRKMS++V+ML WNRAWPEPLL
Sbjct: 364 IDRCESNYASFNVLMELTWDEVLSRGTKHFSEEFSRFCDRKMSDVVSMLSWNRAWPEPLL 423

Query: 431 QAFFSASKSVWLLHLLANSVHPNLPIFRVDKEADFDSVYMEDMGGDKARKLIPSLVRIMI 483
           QAFF ASKSVWL+HLLANSV+P L IFRV+K+  FD +YME+ GG++ +    SLVR M+
Sbjct: 424 QAFFGASKSVWLVHLLANSVNPGLQIFRVEKDDRFDPIYMEETGGERFK----SLVRAMV 472

BLAST of HG10009250 vs. TAIR 10
Match: AT5G12900.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G12330.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 249.6 bits (636), Expect = 5.3e-66
Identity = 137/347 (39.48%), Postives = 216/347 (62.25%), Query Frame = 0

Query: 153 RKSPKSINESSALTAREEQWR-------TAVTELSQKLVQATRKRDEAIMEASRLKYAMA 212
           ++   SI  S ++T + E+         + V +L ++L++A R RD A+ + S +K ++ 
Sbjct: 218 KQKSNSIFNSPSITEKSEEVSEVLKDSGSGVEKLKRELMEANRSRDAALTQVSEMKSSLG 277

Query: 213 ELEKKLDKLETYCHSLKSGIEEC----SGNSPSPCQIGKYNQIQSFQQRNQKQVIEHFLV 272
           EL +KL  LE+YC +LK  + E     S  +      GK N   S    +++ ++E FL 
Sbjct: 278 ELSEKLQYLESYCDNLKKALREATEVVSQENSGGRSSGKKN---SEMPVSEEVMVEGFLQ 337

Query: 273 SVSESRSSIRLLSRSLTLQLRHVGAKVYERISVLLQPYDIKISFSKNPRSMLFYLEALLN 332
            VSE+R SI+   ++L  ++    + +   I+ LLQP+++  + SK  + + ++LEA+++
Sbjct: 338 IVSEARLSIKQFLKTLVSEIDEEDSTLIGNINTLLQPHNLSFT-SKYSKIIQYHLEAIIS 397

Query: 333 QAFFEDFESIGFQKNASTQVLNPIERCEANFGCFNLLHELTWEEVLSKGTKHFSEDFSRF 392
           Q+ ++DFE+  FQKN   ++L+P +  +ANF  F  L  L+W EVL KGTK++S++FSRF
Sbjct: 398 QSVYQDFENCVFQKNGKPKLLDPEQDRQANFSSFASLRNLSWNEVLKKGTKYYSDEFSRF 457

Query: 393 CDRKMSEIVAMLGWNRAWPEPLLQAFFSASKSVWLLHLLANSVHPNLPIFRVDKEADFDS 452
           CD KMS I+  L W R W E +LQAFF A+K VWLLHLLA S +P L I RV++  +F+S
Sbjct: 458 CDEKMSLIITTLNWTRPWSEQMLQAFFVAAKCVWLLHLLAFSFNPALGILRVEENREFES 517

Query: 453 VYMEDMGGDKARKLI---PSLVRIMIAPGFYVYGSVVKCKVLCRYNA 486
            +MEDMG D+ R  +   P+ V++M+ PGFYV   V++CKVLCRY +
Sbjct: 518 SFMEDMGADRQRSALSRGPARVKVMVMPGFYVLDRVLRCKVLCRYKS 560

BLAST of HG10009250 vs. TAIR 10
Match: AT2G45260.1 (Plant protein of unknown function (DUF641) )

HSP 1 Score: 56.6 bits (135), Expect = 6.5e-08
Identity = 30/77 (38.96%), Postives = 45/77 (58.44%), Query Frame = 0

Query: 405 QAFFSASKSVWLLHLLANSVHPNLPIFRVDKEADFDSVYMEDMGGDKA--RKLIPSLVRI 464
           QAF   +KS+W+LH LA S  P   IF+V K ++F   YME +  +     K     V +
Sbjct: 340 QAFLKLAKSIWILHRLAYSFDPAAKIFQVKKGSEFSDSYMESVVKNIVVDEKEENPRVGL 399

Query: 465 MIAPGFYVYGSVVKCKV 480
           M+ PGF++ GSV++ +V
Sbjct: 400 MVMPGFWIGGSVIQSRV 416

BLAST of HG10009250 vs. TAIR 10
Match: AT5G58960.1 (Plant protein of unknown function (DUF641) )

HSP 1 Score: 53.9 bits (128), Expect = 4.2e-07
Identity = 52/188 (27.66%), Postives = 85/188 (45.21%), Query Frame = 0

Query: 314 FYLEALLNQAFFEDFESIGFQKNAS-TQVLNPIERCEANFGCFNLLHELTWEEVLS-KGT 373
           F LE+ + +  F+ F+   F  + S + ++NP +     F  F  +  +   E+L    T
Sbjct: 370 FALESYICRKIFQGFDHETFYMDGSLSSLINPDQYRRDCFAQFKDMKAMDPMELLGILPT 429

Query: 374 KHFSEDFSRFCDRKMSEIV------AMLGWNR------AWPEPLLQ---AFFSASKSVWL 433
            H    F +FC +K   I+      ++ G +       A   P  Q    F   +K+VWL
Sbjct: 430 CH----FGKFCSKKYLSIIHQKMEESLFGDSEQRELVVAGNHPRSQFYGEFLGLAKAVWL 489

Query: 434 LHLLANSVHPNLPIFRVDKEADFDSVYMED-MGGDKARKLIPSLVRIMIAPGFYV----Y 480
           LHLLA S+ P+   F  ++ A+F S YME  +     R     +V   + PGF +     
Sbjct: 490 LHLLAFSLDPSPSHFEANRGAEFHSQYMESVVRFSDGRVPAGQVVGFPVCPGFKLSHQGK 549

BLAST of HG10009250 vs. TAIR 10
Match: AT5G58960.2 (Plant protein of unknown function (DUF641) )

HSP 1 Score: 53.9 bits (128), Expect = 4.2e-07
Identity = 52/188 (27.66%), Postives = 85/188 (45.21%), Query Frame = 0

Query: 314 FYLEALLNQAFFEDFESIGFQKNAS-TQVLNPIERCEANFGCFNLLHELTWEEVLS-KGT 373
           F LE+ + +  F+ F+   F  + S + ++NP +     F  F  +  +   E+L    T
Sbjct: 295 FALESYICRKIFQGFDHETFYMDGSLSSLINPDQYRRDCFAQFKDMKAMDPMELLGILPT 354

Query: 374 KHFSEDFSRFCDRKMSEIV------AMLGWNR------AWPEPLLQ---AFFSASKSVWL 433
            H    F +FC +K   I+      ++ G +       A   P  Q    F   +K+VWL
Sbjct: 355 CH----FGKFCSKKYLSIIHQKMEESLFGDSEQRELVVAGNHPRSQFYGEFLGLAKAVWL 414

Query: 434 LHLLANSVHPNLPIFRVDKEADFDSVYMED-MGGDKARKLIPSLVRIMIAPGFYV----Y 480
           LHLLA S+ P+   F  ++ A+F S YME  +     R     +V   + PGF +     
Sbjct: 415 LHLLAFSLDPSPSHFEANRGAEFHSQYMESVVRFSDGRVPAGQVVGFPVCPGFKLSHQGK 474

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038875926.11.2e-25091.67IRK-interacting protein [Benincasa hispida][more]
XP_004147608.34.2e-24389.33IRK-interacting protein [Cucumis sativus] >KGN49712.2 hypothetical protein Csa_0... [more]
XP_008437154.14.0e-24190.32PREDICTED: IRK-interacting protein [Cucumis melo] >KAA0042846.1 IRK-interacting ... [more]
XP_022922117.11.7e-23989.90IRK-interacting protein-like [Cucurbita moschata][more]
KAG6579443.12.2e-23989.90IRK-interacting protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG701... [more]
Match NameE-valueIdentityDescription
Q9LXU97.4e-6539.48IRK-interacting protein OS=Arabidopsis thaliana OX=3702 GN=IRKI PE=1 SV=1[more]
F4KGE86.0e-0627.66Protein GRAVITROPIC IN THE LIGHT 1 OS=Arabidopsis thaliana OX=3702 GN=GIL1 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A0A0KMS31.6e-24389.84Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G158500 PE=4 SV=1[more]
A0A5A7TLI41.9e-24190.32IRK-interacting protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A1S3ATV61.9e-24190.32IRK-interacting protein OS=Cucumis melo OX=3656 GN=LOC103482662 PE=4 SV=1[more]
A0A6J1E2B78.1e-24089.90IRK-interacting protein-like OS=Cucurbita moschata OX=3662 GN=LOC111430185 PE=4 ... [more]
A0A6J1I2695.8e-23889.09IRK-interacting protein-like OS=Cucurbita maxima OX=3661 GN=LOC111469159 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT1G12330.11.0e-13855.02unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplas... [more]
AT5G12900.15.3e-6639.48unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT2G45260.16.5e-0838.96Plant protein of unknown function (DUF641) [more]
AT5G58960.14.2e-0727.66Plant protein of unknown function (DUF641) [more]
AT5G58960.24.2e-0727.66Plant protein of unknown function (DUF641) [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 176..224
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..110
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..30
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 31..48
NoneNo IPR availablePANTHERPTHR31029:SF4CYCLIN-DEPENDENT KINASE-LIKE PROTEINcoord: 11..484
IPR042316IRK-interacting protein-likePANTHERPTHR31029CYCLIN-DEPENDENT KINASE-LIKE PROTEINcoord: 11..484
IPR001363Proteinase inhibitor I25C, fetuin, conserved sitePROSITEPS01255FETUIN_2coord: 212..221

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10009250.1HG10009250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
biological_process GO:0016192 vesicle-mediated transport
cellular_component GO:0005615 extracellular space
molecular_function GO:0016301 kinase activity