HG10009221 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10009221
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptiongeraniol 8-hydroxylase-like
LocationChr06: 3701768 .. 3704827 (+)
RNA-Seq ExpressionHG10009221
SyntenyHG10009221
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTATAAGATAACAAAGGCACGTGGTTGGGTATTCAAGTCCTATAAAAAAAGGTAACTAAATTGTGTATGGAGCAAGTGGAAGAAAAGAGAGAAATAAGATATGGAGTTAATTATTTCCATGCTCTTTCTTTCCTTGATCTCATGTCTTTCCCTTCTTCTACTCTTCAAATCAAGTACCAAAAGAAGCCATTCAAAGCTCCCTCCTGGGCCAAAAGGTCACTTAGTCATTGGAAATTTAATGGAAATTGGTGAAAAACCCCACCAATCTCTAGCCAATCTAGCCAAGTCCCATGGCCCCATCATGAGCCTCAAACTAGGCCAAATGACTTCCATTGTGATTTCCTCAGCAGCCATGGCAAAGGAAGTCCTCCAAACCCATGACCAACAATTATGCGACCGTACAATCCCACACTCGTCCACAGTTTACGACCACGATAAACTTGGTTTCCCATGGCTTCCAGTGTCCGACCTCTGGAGAACCCTTGACTCAACCGAGATCATTCGTCGAAAGCAAATCCAACAACTTCTAACCTACATACGAGAAAGCGCTGTGAAAGGCGAGGTTGTAGATATTGGAAGAGGTGTGTTTGTGGCCACACTCAACATGCTTTCAAACACGATTTTTTCGGTAGATTTGGCGAACCTTAATTCAGAATGGGCTAAAGATTTTAAAGAGACTGTGTGGGGAATTATGGAGGAGTCTGGGAGAATGGACATAGGAGATTATTTTCCAGTGTTGAAGAAGATGGATATTCAAGGTAGTCGAAGGCCTATGATGGTTTATATTAAAAAGTTTTTGGATATGATTGGTGAGATGATCGACAAGCGGCTGGAGTTGCAAGAAATGGACGAAAATCATGATGTGCTTTACAATCTTCTCAATCTTGCAAAAGAAAATAATGGCTCCATGTTTGACGTGTATTTAATCAAGCATTTGATACTGGTAAATCTCTCTCTACCTTTGCCCAAGAAATGAACTAATCATGATATGTTTTTTTTTTATTATTATTATTTTCTAACTTTATTTTACGAATTGAAAAATTCTTTAGTGCATATCTACACTCACATTTCTTCTCTCTCTCCTTTTTTTTTTTAATATTTATTTTTTTTTTTTTTGGATTTGGGGTGGTCTTCAAAATGGATTGCACTTAAATACGCATTAATTTTTGTTAACAGTAGTTTTAAATATTTGATTTGAGAATAACCCATTTGCATATAAAATATCGGTTTTTATTTAAAATTTGAAAGAAAATGTGGTTAAAATATTATTTTGCCCTTTGTTTCATTCCATTTAGTGTTTGTGCGTATTAATAAATGAGTGTAAATTAACCATGCTTAATTCTTCGTTAATGAAGACTAGCTATATAAGTAGCCACATGTCAAATACATGACAACTCAGCAAGATTTAAAATATAACAAACTATTTAATAGTATATGTTTCAATAAACTTTTAAACGGGTCTATGTTAGTTAATTGTACATCTATTTGGATTTTTATTTTATAGATTTTAGGAATATATCTCCCTTCTCTTTAACTAATTTAACTCATTTTTTATAAAAATTAACTTTCAAGAACTAAATTTAAGATTTATTAAAATACAAAAATTAAAATTAAGATTTATTAAAAATACAAAAATAAAATTTAAAAAATTAATTAAGAGGACATAGACTAAAATGGAATGACATCCACGAAACTAAATAATATTTCAATCAAAGAATACTAAACGGTTAGAACAATGCTTCCTTGTCCACTGAGAAATTTGATATTGAGAGAGAGAAGAGAGCTTATTTTAATTTTTCAATAAAAAATAAATTTATGGTAAAATGTCATTGTCTGTCAATAATAGACACTAACAGAAATTTATAGTGTAATGACATCCAAAGTTAGTTTTTATTGAATATATACTATTAAGAAAATTTCTTATATTATTAAAACATAACATTTTCTTATATTTATAAATATTTTAGCTCATTTTTTTATATTTGAAATAGCTCAAAAAGTTAAATGATTGACAAAAAAAAACAGGAAAAAACCTTCATAGCAACAAATAGAGATTTATTAGTGTCTCCACGAAGTGATTTGATAAGTGTTTAAAAACTTTTTTCATTAAAACTATTTTTTTTTGTAACCACTTTTCGATTATAGTTTTAAGGTCTGTTTGGATTGATTTTTTAAGTGTTTATAAATATTTTTTTTACTTATAAACACTTAGAAAATCAATTCAAATGCAGTCTTATTTTTTTTAAAAAAAATATTTAACATATCACCGACTCAATCTATTTTTGTACAACTTACCAAATTACTATTTTTTTTAATATATATATATAAAATTTGTGTGGTTGAAGTTGAAGTGGAGTGCACTGAATTATCACAATTGTATTATTTCGCATCATTTTGTAGACAACTACTTGCCAGATTTTGTTGTTTATATACTTTGAGTCCACAAAAAAGGTTCTATTCCCAGCTGGTACTGACAACATCCACAGTAGAATGGGCTATGGCAGAGCTATTAAGGAACCCAAAAACTCTATCAAAGGCCAAAACAGAACTAATGGAAATCGTGGGAAAAGACAACCCAGTTGAGGAATCAGATTTCTCAAGGCTCCCTTTTCTTCAAGCAGTTGTAAAAGAAACTCTACGATTGCACCCACCAGCTCCTTTGCTACTCCCACGAAAGGCAAGACAAGACACTGAAATTGGAGGCTTCATAATTCCAAAAGATGCTCAAGTTCTTGTGAATGCATGGGACATTCAAAGGGACAAAAACATTTGGAATGATGCTGATTTGTTCAAACCAGAAAGGTTCTTAGAGTTAGAAAATTGCATTGATGATAAAGGTAGAAACTTGGAGTTGATTCCATTTGGTGCTGGAAGAAGAATTTGCCCTGGATTGCCTTTGGCTACTAGAATGGTTCATTGGATTCTTGCTTCTTTAATTCACTCTTTTGATTGGAAACTTGAAGATGGAATTACACCCAAAACCATGAATATGGATGATAAGTTTGGCCTTACCCTGGCTATGGCCCATCCTCTCAAAGCTATCCCTATCATTGTTTGA

mRNA sequence

ATGTATAAGATAACAAAGGCACGTGGTTGGGTATTCAAGTCCTATAAAAAAAGTACCAAAAGAAGCCATTCAAAGCTCCCTCCTGGGCCAAAAGGTCACTTAGTCATTGGAAATTTAATGGAAATTGGTGAAAAACCCCACCAATCTCTAGCCAATCTAGCCAAGTCCCATGGCCCCATCATGAGCCTCAAACTAGGCCAAATGACTTCCATTGTGATTTCCTCAGCAGCCATGGCAAAGGAAGTCCTCCAAACCCATGACCAACAATTATGCGACCGTACAATCCCACACTCGTCCACAGTTTACGACCACGATAAACTTGGTTTCCCATGGCTTCCAGTGTCCGACCTCTGGAGAACCCTTGACTCAACCGAGATCATTCGTCGAAAGCAAATCCAACAACTTCTAACCTACATACGAGAAAGCGCTGTGAAAGGCGAGGTTGTAGATATTGGAAGAGGTGTGTTTGTGGCCACACTCAACATGCTTTCAAACACGATTTTTTCGGTAGATTTGGCGAACCTTAATTCAGAATGGGCTAAAGATTTTAAAGAGACTGTGTGGGGAATTATGGAGGAGTCTGGGAGAATGGACATAGGAGATTATTTTCCAGTGTTGAAGAAGATGGATATTCAAGGTAGTCGAAGGCCTATGATGGTTTATATTAAAAAGTTTTTGGATATGATTGGTGAGATGATCGACAAGCGGCTGGAGTTGCAAGAAATGGACGAAAATCATGATGTGCTTTACAATCTTCTCAATCTTGCAAAAGAAAATAATGGCTCCATGTTTGACGTGTATTTAATCAAGCATTTGATACTGCTGGTACTGACAACATCCACAGTAGAATGGGCTATGGCAGAGCTATTAAGGAACCCAAAAACTCTATCAAAGGCCAAAACAGAACTAATGGAAATCGTGGGAAAAGACAACCCAGTTGAGGAATCAGATTTCTCAAGGCTCCCTTTTCTTCAAGCAGTTGTAAAAGAAACTCTACGATTGCACCCACCAGCTCCTTTGCTACTCCCACGAAAGGCAAGACAAGACACTGAAATTGGAGGCTTCATAATTCCAAAAGATGCTCAAGTTCTTGTGAATGCATGGGACATTCAAAGGGACAAAAACATTTGGAATGATGCTGATTTGTTCAAACCAGAAAGGTTCTTAGAGTTAGAAAATTGCATTGATGATAAAGGTAGAAACTTGGAGTTGATTCCATTTGGTGCTGGAAGAAGAATTTGCCCTGGATTGCCTTTGGCTACTAGAATGGTTCATTGGATTCTTGCTTCTTTAATTCACTCTTTTGATTGGAAACTTGAAGATGGAATTACACCCAAAACCATGAATATGGATGATAAGTTTGGCCTTACCCTGGCTATGGCCCATCCTCTCAAAGCTATCCCTATCATTGTTTGA

Coding sequence (CDS)

ATGTATAAGATAACAAAGGCACGTGGTTGGGTATTCAAGTCCTATAAAAAAAGTACCAAAAGAAGCCATTCAAAGCTCCCTCCTGGGCCAAAAGGTCACTTAGTCATTGGAAATTTAATGGAAATTGGTGAAAAACCCCACCAATCTCTAGCCAATCTAGCCAAGTCCCATGGCCCCATCATGAGCCTCAAACTAGGCCAAATGACTTCCATTGTGATTTCCTCAGCAGCCATGGCAAAGGAAGTCCTCCAAACCCATGACCAACAATTATGCGACCGTACAATCCCACACTCGTCCACAGTTTACGACCACGATAAACTTGGTTTCCCATGGCTTCCAGTGTCCGACCTCTGGAGAACCCTTGACTCAACCGAGATCATTCGTCGAAAGCAAATCCAACAACTTCTAACCTACATACGAGAAAGCGCTGTGAAAGGCGAGGTTGTAGATATTGGAAGAGGTGTGTTTGTGGCCACACTCAACATGCTTTCAAACACGATTTTTTCGGTAGATTTGGCGAACCTTAATTCAGAATGGGCTAAAGATTTTAAAGAGACTGTGTGGGGAATTATGGAGGAGTCTGGGAGAATGGACATAGGAGATTATTTTCCAGTGTTGAAGAAGATGGATATTCAAGGTAGTCGAAGGCCTATGATGGTTTATATTAAAAAGTTTTTGGATATGATTGGTGAGATGATCGACAAGCGGCTGGAGTTGCAAGAAATGGACGAAAATCATGATGTGCTTTACAATCTTCTCAATCTTGCAAAAGAAAATAATGGCTCCATGTTTGACGTGTATTTAATCAAGCATTTGATACTGCTGGTACTGACAACATCCACAGTAGAATGGGCTATGGCAGAGCTATTAAGGAACCCAAAAACTCTATCAAAGGCCAAAACAGAACTAATGGAAATCGTGGGAAAAGACAACCCAGTTGAGGAATCAGATTTCTCAAGGCTCCCTTTTCTTCAAGCAGTTGTAAAAGAAACTCTACGATTGCACCCACCAGCTCCTTTGCTACTCCCACGAAAGGCAAGACAAGACACTGAAATTGGAGGCTTCATAATTCCAAAAGATGCTCAAGTTCTTGTGAATGCATGGGACATTCAAAGGGACAAAAACATTTGGAATGATGCTGATTTGTTCAAACCAGAAAGGTTCTTAGAGTTAGAAAATTGCATTGATGATAAAGGTAGAAACTTGGAGTTGATTCCATTTGGTGCTGGAAGAAGAATTTGCCCTGGATTGCCTTTGGCTACTAGAATGGTTCATTGGATTCTTGCTTCTTTAATTCACTCTTTTGATTGGAAACTTGAAGATGGAATTACACCCAAAACCATGAATATGGATGATAAGTTTGGCCTTACCCTGGCTATGGCCCATCCTCTCAAAGCTATCCCTATCATTGTTTGA

Protein sequence

MYKITKARGWVFKSYKKSTKRSHSKLPPGPKGHLVIGNLMEIGEKPHQSLANLAKSHGPIMSLKLGQMTSIVISSAAMAKEVLQTHDQQLCDRTIPHSSTVYDHDKLGFPWLPVSDLWRTLDSTEIIRRKQIQQLLTYIRESAVKGEVVDIGRGVFVATLNMLSNTIFSVDLANLNSEWAKDFKETVWGIMEESGRMDIGDYFPVLKKMDIQGSRRPMMVYIKKFLDMIGEMIDKRLELQEMDENHDVLYNLLNLAKENNGSMFDVYLIKHLILLVLTTSTVEWAMAELLRNPKTLSKAKTELMEIVGKDNPVEESDFSRLPFLQAVVKETLRLHPPAPLLLPRKARQDTEIGGFIIPKDAQVLVNAWDIQRDKNIWNDADLFKPERFLELENCIDDKGRNLELIPFGAGRRICPGLPLATRMVHWILASLIHSFDWKLEDGITPKTMNMDDKFGLTLAMAHPLKAIPIIV
Homology
BLAST of HG10009221 vs. NCBI nr
Match: XP_004147600.2 (geraniol 8-hydroxylase [Cucumis sativus] >KGN50170.1 hypothetical protein Csa_000342 [Cucumis sativus])

HSP 1 Score: 753.4 bits (1944), Expect = 1.1e-213
Identity = 375/477 (78.62%), Postives = 416/477 (87.21%), Query Frame = 0

Query: 15  YKKSTKRSHSKLPPGPKGHLVIGNLMEIGEKPHQSLANLAKSHGPIMSLKLGQMTSIVIS 74
           +  STKR+HSKLPPGPKG+LVIGNLM+IG+KPHQSLANLAKSHGPIMSLKLGQMTSIVIS
Sbjct: 24  FNSSTKRTHSKLPPGPKGYLVIGNLMDIGDKPHQSLANLAKSHGPIMSLKLGQMTSIVIS 83

Query: 75  SAAMAKEVLQTHDQQLCDRTIPHSSTVYDHDKLGFPWLPVSDLWRT-------------- 134
           SAAMAKEVLQTHDQQLCDRTIP+SSTVYDHDKLGF WLPV D+WRT              
Sbjct: 84  SAAMAKEVLQTHDQQLCDRTIPYSSTVYDHDKLGFVWLPVCDVWRTLRKVCNNHMFSHKI 143

Query: 135 LDSTEIIRRKQIQQLLTYIRESAVKGEVVDIGRGVFVATLNMLSNTIFSVDLANLNSEWA 194
           LDST+IIR+KQIQ+LL  +RE A+KGE VDIG+  FV  LNMLSN +FSVDLA+ NSEWA
Sbjct: 144 LDSTKIIRQKQIQRLLDNVRERALKGEAVDIGKAAFVTILNMLSNMVFSVDLADPNSEWA 203

Query: 195 KDFKETVWGIMEESGRMDIGDYFPVLKKMDIQGSRRPMMVYIKKFLDMIGEMIDKRLELQ 254
           KD KETVWGIMEESG+ DIGDYFP+ K MDI+GSRR MMVYIKKFLDMIG+MI+KR+EL+
Sbjct: 204 KDLKETVWGIMEESGKPDIGDYFPLFKTMDIKGSRRRMMVYIKKFLDMIGDMIEKRMELE 263

Query: 255 EMDENHDVLYNLLNLAKENNGSMFDVYLIKHLILLVL------TTSTVEWAMAELLRNPK 314
            ++ENHD+LYNLLNLAKEN+ SMFDVYLIKHLIL++L      TTS VEWAMAELL+NP+
Sbjct: 264 GVEENHDMLYNLLNLAKENDDSMFDVYLIKHLILVLLPAGTDTTTSMVEWAMAELLKNPE 323

Query: 315 TLSKAKTELMEIVGKDNPVEESDFSRLPFLQAVVKETLRLHPPAPLLLPRKARQDTEIGG 374
            LSKA+ ELME+VGK+ P+EESD  +LPFLQA+ KETLRLHPP PLLLPRKARQDTEIGG
Sbjct: 324 ALSKARIELMEVVGKNRPIEESDILKLPFLQAIAKETLRLHPPVPLLLPRKARQDTEIGG 383

Query: 375 FIIPKDAQVLVNAWDIQRDKNIWNDADLFKPERFLELENCIDDKGRNLELIPFGAGRRIC 434
           F+IPKDAQV+VNAW IQRDKNIW D +LFKPERFLEL   ID KGRN+ELIPFGAGRRIC
Sbjct: 384 FVIPKDAQVIVNAWYIQRDKNIWEDGELFKPERFLELSE-IDYKGRNMELIPFGAGRRIC 443

Query: 435 PGLPLATRMVHWILASLIHSFDWKLEDGITPKTMNMDDKFGLTLAMAHPLKAIPIIV 472
           PGLPLA RM HWILASLIHSFDWKLEDGITPK MNMD+K GLTL MAHPLKAIPIIV
Sbjct: 444 PGLPLANRMGHWILASLIHSFDWKLEDGITPKNMNMDEKVGLTLVMAHPLKAIPIIV 499

BLAST of HG10009221 vs. NCBI nr
Match: XP_008437176.1 (PREDICTED: geraniol 8-hydroxylase-like [Cucumis melo])

HSP 1 Score: 746.1 bits (1925), Expect = 1.8e-211
Identity = 371/477 (77.78%), Postives = 415/477 (87.00%), Query Frame = 0

Query: 15  YKKSTKRSHSKLPPGPKGHLVIGNLMEIGEKPHQSLANLAKSHGPIMSLKLGQMTSIVIS 74
           +  S KR+HSKLPPGPKG+LVIGNLM+IG+KPHQSLANLAKSHGPIMSLKLGQMTSIVIS
Sbjct: 23  FNSSNKRTHSKLPPGPKGYLVIGNLMDIGDKPHQSLANLAKSHGPIMSLKLGQMTSIVIS 82

Query: 75  SAAMAKEVLQTHDQQLCDRTIPHSSTVYDHDKLGFPWLPVSDLWRT-------------- 134
           SAAMAKEVLQTHDQQLCDRTIP SS VYDHDKLGFPWLPVSD+WRT              
Sbjct: 83  SAAMAKEVLQTHDQQLCDRTIPCSSAVYDHDKLGFPWLPVSDVWRTLRKVCNNHMFSQKI 142

Query: 135 LDSTEIIRRKQIQQLLTYIRESAVKGEVVDIGRGVFVATLNMLSNTIFSVDLANLNSEWA 194
           LDSTEIIRRKQIQ+LL  +RE A+KGE VDI +  F   LNMLSN IFSVDLA+ NSEWA
Sbjct: 143 LDSTEIIRRKQIQRLLDNVREKALKGEAVDIEKAAFGTILNMLSNMIFSVDLADPNSEWA 202

Query: 195 KDFKETVWGIMEESGRMDIGDYFPVLKKMDIQGSRRPMMVYIKKFLDMIGEMIDKRLELQ 254
           K+ KETVWGIMEESG+ DIGDYFP+LKKMDI+GSRR MMVYIKKFLDMIG+M++KR+EL+
Sbjct: 203 KELKETVWGIMEESGKPDIGDYFPLLKKMDIKGSRRRMMVYIKKFLDMIGDMMEKRMELE 262

Query: 255 EMDENHDVLYNLLNLAKENNGSMFDVYLIKHLILLVL------TTSTVEWAMAELLRNPK 314
           +M+ENHD+LYNLLNLA EN+GS+FD+YLIKHLIL++L      TTS VEWAMAELL+NP+
Sbjct: 263 DMEENHDMLYNLLNLATENDGSIFDIYLIKHLILVLLPAGSDTTTSMVEWAMAELLKNPE 322

Query: 315 TLSKAKTELMEIVGKDNPVEESDFSRLPFLQAVVKETLRLHPPAPLLLPRKARQDTEIGG 374
            LSKA+ ELME+VGK+ P+EESD S+LPFLQA+ KETLRLHPP PLLLPRKAR+DTEIGG
Sbjct: 323 ALSKARRELMEVVGKNRPIEESDLSKLPFLQAIAKETLRLHPPVPLLLPRKAREDTEIGG 382

Query: 375 FIIPKDAQVLVNAWDIQRDKNIWNDADLFKPERFLELENCIDDKGRNLELIPFGAGRRIC 434
           FI+PKDAQV+VNAW IQRD++IW D +LFKPERFLEL   ID KGRN+ELIPFGAGRRIC
Sbjct: 383 FIVPKDAQVIVNAWYIQRDRDIWEDGELFKPERFLELSE-IDYKGRNMELIPFGAGRRIC 442

Query: 435 PGLPLATRMVHWILASLIHSFDWKLEDGITPKTMNMDDKFGLTLAMAHPLKAIPIIV 472
           PGLPLA RM  WIL SLIHSFDWKLEDGITPK MNMD+K GLTL MAHPLKAIPIIV
Sbjct: 443 PGLPLANRMGLWILGSLIHSFDWKLEDGITPKNMNMDEKVGLTLVMAHPLKAIPIIV 498

BLAST of HG10009221 vs. NCBI nr
Match: KAA0042823.1 (geraniol 8-hydroxylase-like [Cucumis melo var. makuwa])

HSP 1 Score: 743.8 bits (1919), Expect = 9.1e-211
Identity = 369/477 (77.36%), Postives = 416/477 (87.21%), Query Frame = 0

Query: 15  YKKSTKRSHSKLPPGPKGHLVIGNLMEIGEKPHQSLANLAKSHGPIMSLKLGQMTSIVIS 74
           +  S KR+HSKLPPGPKG+LVIGNLM+IG+KPHQSLANLAKSHGPIMSLKLGQMTSIVIS
Sbjct: 23  FNSSNKRTHSKLPPGPKGYLVIGNLMDIGDKPHQSLANLAKSHGPIMSLKLGQMTSIVIS 82

Query: 75  SAAMAKEVLQTHDQQLCDRTIPHSSTVYDHDKLGFPWLPVSDLWRT-------------- 134
           SAAMAKEVLQTHDQQLCDRTIP SS VYDHDKLGFPWLPVSD+WRT              
Sbjct: 83  SAAMAKEVLQTHDQQLCDRTIPCSSAVYDHDKLGFPWLPVSDVWRTLRKVCNNHMFSQKI 142

Query: 135 LDSTEIIRRKQIQQLLTYIRESAVKGEVVDIGRGVFVATLNMLSNTIFSVDLANLNSEWA 194
           LDSTEIIRRKQIQ+L+  +RE A++GE VDI +  F   LNMLSN IFSVDLA+ NSEWA
Sbjct: 143 LDSTEIIRRKQIQRLVDNVREKALEGEAVDIEKAAFGTILNMLSNMIFSVDLADPNSEWA 202

Query: 195 KDFKETVWGIMEESGRMDIGDYFPVLKKMDIQGSRRPMMVYIKKFLDMIGEMIDKRLELQ 254
           K+ KETVWGIMEESG+ DIGDYFP+LKKMDI+GSRR MMVYIKKFLDMIG+M++KR+EL+
Sbjct: 203 KELKETVWGIMEESGKPDIGDYFPLLKKMDIKGSRRRMMVYIKKFLDMIGDMMEKRMELE 262

Query: 255 EMDENHDVLYNLLNLAKENNGSMFDVYLIKHLILLVL------TTSTVEWAMAELLRNPK 314
           +M+ENHD+LYNLLNLA EN+GS+FD+YLIKHLIL++L      TTS VEWAMAELL+NP+
Sbjct: 263 DMEENHDMLYNLLNLATENDGSIFDIYLIKHLILVLLPAGSDTTTSMVEWAMAELLKNPE 322

Query: 315 TLSKAKTELMEIVGKDNPVEESDFSRLPFLQAVVKETLRLHPPAPLLLPRKARQDTEIGG 374
            LSKA+ ELME++GK+ P+EESD S+LPFLQA+ KETLRLHPP PLLLPRKAR+DTEIGG
Sbjct: 323 ALSKARRELMEVMGKNRPIEESDLSKLPFLQAIAKETLRLHPPVPLLLPRKAREDTEIGG 382

Query: 375 FIIPKDAQVLVNAWDIQRDKNIWNDADLFKPERFLELENCIDDKGRNLELIPFGAGRRIC 434
           FI+PKDAQV+VNAW IQRDK+IW D++LFKPERFLEL   ID KGRN+ELIPFGAGRRIC
Sbjct: 383 FIVPKDAQVIVNAWYIQRDKDIWEDSELFKPERFLELSE-IDYKGRNMELIPFGAGRRIC 442

Query: 435 PGLPLATRMVHWILASLIHSFDWKLEDGITPKTMNMDDKFGLTLAMAHPLKAIPIIV 472
           PGLPLA RM  WIL SLIHSFDWKLEDGITPK MNMD+K GLTL MAHPLKAIPIIV
Sbjct: 443 PGLPLANRMGLWILGSLIHSFDWKLEDGITPKNMNMDEKVGLTLVMAHPLKAIPIIV 498

BLAST of HG10009221 vs. NCBI nr
Match: XP_022159618.1 (geraniol 8-hydroxylase-like [Momordica charantia])

HSP 1 Score: 670.6 bits (1729), Expect = 9.7e-189
Identity = 347/486 (71.40%), Postives = 396/486 (81.48%), Query Frame = 0

Query: 11  VFKSYKKSTKRSHSKLPPGPKGHLVIGNLMEIGEKPHQSLANLAKSHGPIMSLKLGQMTS 70
           +F S + S+++  +K PPGPKG+ +IGNL+E+G+KPHQSLA LA SHGPIM LKLGQMT+
Sbjct: 19  LFNSKRSSSRQWKNKRPPGPKGYPLIGNLLEMGDKPHQSLAILAASHGPIMRLKLGQMTT 78

Query: 71  IVISSAAMAKEVLQTHDQQLCDRTIPHSSTVYDHDKLGFPWLPVSDLWRT---------- 130
           IVISSAAMAK+VLQTHDQ L DRT+PHSSTVYDHDKLGFPWLPVSDLWRT          
Sbjct: 79  IVISSAAMAKQVLQTHDQALSDRTVPHSSTVYDHDKLGFPWLPVSDLWRTLRKVCNNHMF 138

Query: 131 ----LDSTEIIRRKQIQQLLTYIRESAVKGEVVDIGRGVFVATLNMLSNTIFSVDLANLN 190
               LDS E IRR  + +LL  IR SA  GE VDI R VF A  NMLSNTIFSVDLA+ N
Sbjct: 139 SHNALDSHEPIRRNNVGRLLADIRRSAHNGESVDIERAVFGAAFNMLSNTIFSVDLADPN 198

Query: 191 SEWAKDFKETVWGIMEESGRMDIGDYFPVLKKMDIQGSRRPMMVYIKKFLDMIGEMIDKR 250
           SEWAK+FKETVWGI+EESG+ D+GDYFPVLK MD++GSRR MMVYIKKFLDMI EMI++R
Sbjct: 199 SEWAKEFKETVWGILEESGKFDVGDYFPVLKWMDLRGSRRRMMVYIKKFLDMIVEMIEER 258

Query: 251 LELQE----MDENH-DVLYNLLNLAKENNGSMFDVYLIKHLILLVL------TTSTVEWA 310
              QE     D  H D+ +NLLNL KEN    FDVYLIKHLIL++       TTSTV+WA
Sbjct: 259 FRRQEFAGGFDGGHRDMFHNLLNLPKENTYPNFDVYLIKHLILVLFPAGTDTTTSTVQWA 318

Query: 311 MAELLRNPKTLSKAKTELMEIVGKDNPVEESDFSRLPFLQAVVKETLRLHPPAPLLLPRK 370
           MAELLRN K L KA+TEL E++GKD P++ESDFSRLPFL+A+VKETLRLHPPAPLLLPRK
Sbjct: 319 MAELLRNRKILLKAQTELREVIGKD-PLKESDFSRLPFLRAIVKETLRLHPPAPLLLPRK 378

Query: 371 ARQDTEIGGFIIPKDAQVLVNAWDIQRDKNIWNDADLFKPERFLELENCIDDKGRNLELI 430
           AR+DT+I GF+IPKDAQV+VNAW+I RDK+IW DA  FKPERFL  E  ID KGRN ELI
Sbjct: 379 AREDTKIEGFLIPKDAQVIVNAWEIGRDKSIWEDASSFKPERFLGSE--IDFKGRNFELI 438

Query: 431 PFGAGRRICPGLPLATRMVHWILASLIHSFDWKLEDGITPKTMNMDDKFGLTLAMAHPLK 472
           PFGAGRRICPGLPLATRMVHWIL SLIHSFDWKLEDGITP+T+NMD+K GLT+ MAHPL+
Sbjct: 439 PFGAGRRICPGLPLATRMVHWILGSLIHSFDWKLEDGITPETINMDEKVGLTVVMAHPLR 498

BLAST of HG10009221 vs. NCBI nr
Match: XP_038874677.1 (geraniol 8-hydroxylase-like [Benincasa hispida])

HSP 1 Score: 613.2 bits (1580), Expect = 1.8e-171
Identity = 310/475 (65.26%), Postives = 378/475 (79.58%), Query Frame = 0

Query: 19  TKRSHSK-LPPGPKGHLVIGNLMEIGEKPHQSLANLAKSHGPIMSLKLGQMTSIVISSAA 78
           T++S+ K LPPGPK H +IGNLMEIG+  HQSLA LA+S+GPIM+LKLGQMTSIVISS +
Sbjct: 44  TRKSNQKQLPPGPKAHFLIGNLMEIGQNLHQSLAKLAESYGPIMTLKLGQMTSIVISSPS 103

Query: 79  MAKEVLQTHDQQLCDRTIPHSSTVYDHDKLGFPWLPVSDLWRT--------------LDS 138
           MAKEVLQTHDQ LCDRT P  +TVY+H K+   WLPV DLWRT              LDS
Sbjct: 104 MAKEVLQTHDQVLCDRTFPDVATVYNHHKVALAWLPVCDLWRTLRKLCNNHMFSHKSLDS 163

Query: 139 TEIIRRKQIQQLLTYIRESAVKGEVVDIGRGVFVATLNMLSNTIFSVDLANLNS---EWA 198
           + IIR K IQ L+  +R+SA KG+ +DI R VF  TL+MLSN IFS+DL++  S   +W 
Sbjct: 164 SYIIRHKVIQHLVADVRKSAAKGDTIDIERLVFSTTLDMLSNMIFSIDLSSKYSNIHKWG 223

Query: 199 KDFKETVWGIMEESGRMDIGDYFPVLKKMDIQGSRRPMMVYIKKFLDMIGEMIDKRLELQ 258
           +DFKETVWGIMEE+G ++IGD+FP+LKKMD+QG R+ MMV++ KFLD+I EMI+KR++  
Sbjct: 224 QDFKETVWGIMEEAGGLNIGDFFPILKKMDLQGCRKRMMVHMNKFLDIIDEMIEKRMKEP 283

Query: 259 EMDENHDVLYNLLNLAKENNGSMFDVYLIKHLILLVL------TTSTVEWAMAELLRNPK 318
           ++ E+ D+L N LNLAKE+    FD+ LIKHLIL++       TTSTVEWAMAELLRNPK
Sbjct: 284 KLGEDIDMLQNFLNLAKESEDPKFDLQLIKHLILVLFPAGTDTTTSTVEWAMAELLRNPK 343

Query: 319 TLSKAKTELMEIVGKDNPVEESDFSRLPFLQAVVKETLRLHPPAPLLLPRKARQDTE-IG 378
            LSKAKTEL E++G+D  ++ESD+ RLPFLQA++KETLRLHPPAPLLLP KA+ DTE IG
Sbjct: 344 MLSKAKTELKEVIGEDMEMKESDYLRLPFLQAIIKETLRLHPPAPLLLPHKAKHDTEIIG 403

Query: 379 GFIIPKDAQVLVNAWDIQRDKNIWNDADLFKPERFLELENCIDDKGRNLELIPFGAGRRI 438
           GF IPK+AQ+LVN W I RDK++W DADLFKPERFL L + +D KGR+ ELIPFG+GRRI
Sbjct: 404 GFEIPKNAQLLVNVWKIGRDKSVWEDADLFKPERFLGLGSKVDYKGRDFELIPFGSGRRI 463

Query: 439 CPGLPLATRMVHWILASLIHSFDWKLEDGITPKTMNMDDKFGLTLAMAHPLKAIP 469
           CPGLPLAT+MVHWIL SLIHSF W+LEDGI P+T+NM++K GLTL MAHP KAIP
Sbjct: 464 CPGLPLATKMVHWILGSLIHSFHWRLEDGIKPETLNMEEKVGLTLVMAHPPKAIP 518

BLAST of HG10009221 vs. ExPASy Swiss-Prot
Match: D1MI46 (Geraniol 8-hydroxylase OS=Swertia mussotii OX=137888 GN=CYP76B10 PE=1 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 3.3e-123
Identity = 220/467 (47.11%), Postives = 322/467 (68.95%), Query Frame = 0

Query: 21  RSHSKLPPGPKGHLVIGNLMEIGEKPHQSLANLAKSHGPIMSLKLGQMTSIVISSAAMAK 80
           R    LPPGP    +IGNL  +G++PH+SLA LAK HGPIM L+LGQ+T+IV++S+ MAK
Sbjct: 27  RKSKNLPPGPSPLPLIGNLHLLGDQPHKSLAKLAKKHGPIMGLQLGQVTTIVVTSSGMAK 86

Query: 81  EVLQTHDQQLCDRTIPHSSTVYDHDKLGFPWLPVSDLWR--------------TLDSTEI 140
           EVLQ  D     R+IP++   +D  K    WLPV+  WR               LD+ + 
Sbjct: 87  EVLQKQDLAFSSRSIPNAIHAHDQYKYSVIWLPVASRWRGLRKALNSNMFSGNRLDANQH 146

Query: 141 IRRKQIQQLLTYIRESAVKGEVVDIGRGVFVATLNMLSNTIFSVDLANLNSEWAKDFKET 200
           +R +++Q+L+ Y R+S+  G+ +D+GR  F  +LN+LSNT+FS DL +  S+ AK+FK+ 
Sbjct: 147 LRSRKVQELIAYCRKSSQTGDAIDVGRAAFRTSLNLLSNTMFSKDLTDPYSDSAKEFKDL 206

Query: 201 VWGIMEESGRMDIGDYFPVLKKMDIQGSRRPMMVYIKKFLDMIGEMIDKRLELQE-MDEN 260
           VW +M E+G+ ++ DYFP+L K+D QG R+ M ++  K L++ G +ID+RL+ ++    N
Sbjct: 207 VWNVMVEAGKPNLVDYFPLLDKVDPQGIRKRMTIHFGKILELFGGLIDERLQQKKAKGVN 266

Query: 261 HDVLYNLLNLAKENNGSMFDVYLIKHLILLVL-----TTSTVEWAMAELLRNPKTLSKAK 320
            DVL  LL  ++E+   +   ++ +  + L +     T+ST+EWAM+E+L+NP+ +  A+
Sbjct: 267 DDVLDVLLTTSEESPEEIDRTHIQRMCLDLFVAGTDTTSSTLEWAMSEMLKNPEKMKAAQ 326

Query: 321 TELMEIVGKDNPVEESDFSRLPFLQAVVKETLRLHPPAPLLLPRKARQDTEIGGFIIPKD 380
            EL +++GK   VEE+D +RLP+L+  +KETLR+HPP PLL+PR+  Q+ E+ G+ +PK+
Sbjct: 327 AELAQVIGKGKAVEEADLARLPYLRCAIKETLRIHPPVPLLIPRRTEQEVEVCGYTVPKN 386

Query: 381 AQVLVNAWDIQRDKNIWNDADLFKPERFLELENCIDDKGRNLELIPFGAGRRICPGLPLA 440
           +QVLVN W I RD  IW D   FKPERFLE E  ++ +G++ ELIPFGAGRRICPGLPLA
Sbjct: 387 SQVLVNVWAISRDDAIWKDPLSFKPERFLESE--LEMRGKDFELIPFGAGRRICPGLPLA 446

Query: 441 TRMVHWILASLIHSFDWKLEDGITPKTMNMDDKFGLTLAMAHPLKAI 468
            RMV  +L SL++SFDWKLE GI PK ++M++KFG+TL  AHPL+A+
Sbjct: 447 VRMVPVMLGSLLNSFDWKLEGGIAPKDLDMEEKFGITLQKAHPLRAV 491

BLAST of HG10009221 vs. ExPASy Swiss-Prot
Match: Q8VWZ7 (Geraniol 8-hydroxylase OS=Catharanthus roseus OX=4058 GN=CYP76B6 PE=1 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 8.2e-122
Identity = 219/468 (46.79%), Postives = 322/468 (68.80%), Query Frame = 0

Query: 21  RSHSKLPPGPKGHLVIGNLMEIGEKPHQSLANLAKSHGPIMSLKLGQMTSIVISSAAMAK 80
           R    LPPGP     IG+L  +G++PH+SLA L+K HGPIMSLKLGQ+T+IVISS+ MAK
Sbjct: 25  RRTKNLPPGPSPLPFIGSLHLLGDQPHKSLAKLSKKHGPIMSLKLGQITTIVISSSTMAK 84

Query: 81  EVLQTHDQQLCDRTIPHSSTVYDHDKLGFPWLPVSDLWRT--------------LDSTEI 140
           EVLQ  D     R++P++   ++  K    WLPV+  WR+              LD+ + 
Sbjct: 85  EVLQKQDLAFSSRSVPNALHAHNQFKFSVVWLPVASRWRSLRKVLNSNIFSGNRLDANQH 144

Query: 141 IRRKQIQQLLTYIRESAVKGEVVDIGRGVFVATLNMLSNTIFSVDLANLNSEWAKDFKET 200
           +R +++Q+L+ Y R+++  GE VD+GR  F  +LN+LSN IFS DL +  S+ AK+FK+ 
Sbjct: 145 LRTRKVQELIAYCRKNSQSGEAVDVGRAAFRTSLNLLSNLIFSKDLTDPYSDSAKEFKDL 204

Query: 201 VWGIMEESGRMDIGDYFPVLKKMDIQGSRRPMMVYIKKFLDMIGEMIDKRLE-LQEMDEN 260
           VW IM E+G+ ++ D+FP+L+K+D QG R  M ++  + L + G ++++RLE  +   E 
Sbjct: 205 VWNIMVEAGKPNLVDFFPLLEKVDPQGIRHRMTIHFGEVLKLFGGLVNERLEQRRSKGEK 264

Query: 261 HDVLYNLLNLAKENNGSMFDVYLIKHLILLVL-----TTSTVEWAMAELLRNPKTLSKAK 320
           +DVL  LL  ++E+   +   ++ +  + L +     T+ST+EWAM+E+L+NP  + K +
Sbjct: 265 NDVLDVLLTTSQESPEEIDRTHIERMCLDLFVAGTDTTSSTLEWAMSEMLKNPDKMKKTQ 324

Query: 321 TELMEIVGKDNPVEESDFSRLPFLQAVVKETLRLHPPAPLLLPRKARQDTEIGGFIIPKD 380
            EL +++G+   +EESD +RLP+L+ V+KETLR+HPP P L+PRK  Q  E+ G+ +PK 
Sbjct: 325 DELAQVIGRGKTIEESDINRLPYLRCVMKETLRIHPPVPFLIPRKVEQSVEVCGYNVPKG 384

Query: 381 AQVLVNAWDIQRDKNIWNDADLFKPERFLELENCIDDKGRNLELIPFGAGRRICPGLPLA 440
           +QVLVNAW I RD+ +W+DA  FKPERF+E E  +D +GR+ ELIPFGAGRRICPGLPLA
Sbjct: 385 SQVLVNAWAIGRDETVWDDALAFKPERFMESE--LDIRGRDFELIPFGAGRRICPGLPLA 444

Query: 441 TRMVHWILASLIHSFDWKLEDGITPKTMNMDDKFGLTLAMAHPLKAIP 469
            R V  +L SL++SF+WKLE G+ PK ++M++KFG+TL  AHPL+A+P
Sbjct: 445 LRTVPLMLGSLLNSFNWKLEGGMAPKDLDMEEKFGITLQKAHPLRAVP 490

BLAST of HG10009221 vs. ExPASy Swiss-Prot
Match: I3PFJ5 (Cytochrome P450 76AD1 OS=Beta vulgaris OX=161934 GN=CYP76AD1 PE=2 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 7.6e-120
Identity = 224/468 (47.86%), Postives = 306/468 (65.38%), Query Frame = 0

Query: 26  LPPGPKGHLVIGNLMEIGEKPHQSLANLAKSHGPIMSLKLGQMTSIVISSAAMAKEVLQT 85
           LPPGPK   +IGN++E+G+KPH+S ANLAK HGP++SL+LG +T+IV+SSA +AKE+   
Sbjct: 32  LPPGPKPLPIIGNILEVGKKPHRSFANLAKIHGPLISLRLGSVTTIVVSSADVAKEMFLK 91

Query: 86  HDQQLCDRTIPHSSTVYDHDKLGFPWLPVSDLWRT--------------LDSTEIIRRKQ 145
            D  L +RTIP+S T  DH KL   WLPVS  WR               LD+ +  R  +
Sbjct: 92  KDHPLSNRTIPNSVTAGDHHKLTMSWLPVSPKWRNFRKITAVHLLSPQRLDACQTFRHAK 151

Query: 146 IQQLLTYIRESAVKGEVVDIGRGVFVATLNMLSNTIFSVDLANLNSEWAKDFKETVWGIM 205
           +QQL  Y++E A KG+ VDIG+  F  +LN+LS   FSV+LA+  S  +++FKE +W IM
Sbjct: 152 VQQLYEYVQECAQKGQAVDIGKAAFTTSLNLLSKLFFSVELAHHKSHTSQEFKELIWNIM 211

Query: 206 EESGRMDIGDYFPVLKKMDIQGSRRPMMVYIKKFLDMIGEMIDKRL----ELQEMDENHD 265
           E+ G+ +  DYFP+L  +D  G RR +     K + +   +I +RL             D
Sbjct: 212 EDIGKPNYADYFPILGCVDPSGIRRRLACSFDKLIAVFQGIICERLAPDSSTTTTTTTDD 271

Query: 266 VLYNLLNLAKENNGSMFDVYLIKHLILLVL------TTSTVEWAMAELLRNPKTLSKAKT 325
           VL  LL L K+N  +M +   I HL++ +       T+ST EW M EL+RNP+ + KA+ 
Sbjct: 272 VLDVLLQLFKQNELTMGE---INHLLVDIFDAGTDTTSSTFEWVMTELIRNPEMMEKAQE 331

Query: 326 ELMEIVGKDNPVEESDFSRLPFLQAVVKETLRLHPPAPLLLPRKARQDTEIGGFIIPKDA 385
           E+ +++GKD  ++ESD   LP+LQA++KETLRLHPP   LLPRKA  D E+ G+I+PKDA
Sbjct: 332 EIKQVLGKDKQIQESDIINLPYLQAIIKETLRLHPPTVFLLPRKADTDVELYGYIVPKDA 391

Query: 386 QVLVNAWDIQRDKNIWNDADLFKPERFLELENCIDDKGRNLELIPFGAGRRICPGLPLAT 445
           Q+LVN W I RD N W +AD+F PERF+  E  ID KGR+  L+PFGAGRRICPG+ LA 
Sbjct: 392 QILVNLWAIGRDPNAWQNADIFSPERFIGCE--IDVKGRDFGLLPFGAGRRICPGMNLAI 451

Query: 446 RMVHWILASLIHSFDWKLEDGITPKTMNMDDKFGLTLAMAHPLKAIPI 470
           RM+  +LA+L+  F+WKLE  I+PK ++MD+KFG+ L    PLK IPI
Sbjct: 452 RMLTLMLATLLQFFNWKLEGDISPKDLDMDEKFGIALQKTKPLKLIPI 494

BLAST of HG10009221 vs. ExPASy Swiss-Prot
Match: W0FKI0 (Drimenol monooxygenase OS=Persicaria hydropiper OX=46901 GN=CYP76AJ1 PE=1 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 4.2e-118
Identity = 220/473 (46.51%), Postives = 312/473 (65.96%), Query Frame = 0

Query: 18  STKRSHSKLPPGPKGHLVIGNLMEIGEKPHQSLANLAKSHGPIMSLKLGQMTSIVISSAA 77
           S++ S  KLPPGP G  ++GNL ++G  PH+SLA LAK HGP+M L++G++  +VISS +
Sbjct: 19  SSRTSKMKLPPGPPGLPIVGNLFDLGSLPHRSLAKLAKLHGPVMCLRMGRLRVVVISSDS 78

Query: 78  MAKEVLQTHDQQLCDRTIPHSSTVYDHDKLGFPWLPVSDLWRTL--------------DS 137
            AKEVLQT D   C+R +  S T   H       LP + LW++L              ++
Sbjct: 79  AAKEVLQTSDTLFCNRFVYDSLTASQHHTFSMALLPPTALWKSLRKISASQLFTNARMNA 138

Query: 138 TEIIRRKQIQQLLTYIRESAVKGEVVDIGRGVFVATLNMLSNTIFSVDLANLNSEWAKDF 197
           ++ +RRKQ++ LL+Y+   +  G  ++I +  F  ++N+LS T FSVDL + +S  + +F
Sbjct: 139 SQHLRRKQLEDLLSYVESCSRSGTAINIAQAAFNTSVNLLSKTFFSVDLIDPSSSNSVEF 198

Query: 198 KETVWGIMEESGRMDIGDYFPVLKKMDIQGSRRPMMVYIKKFLDMIGEMIDKRLELQE-- 257
           KE VW IM ESG  ++ DYFPVL+++D QG+RR M +  +K LD+   MI +RL+ +   
Sbjct: 199 KEMVWQIMLESGTPNLADYFPVLRRIDPQGNRRRMKIQFEKILDLFNTMIRQRLDEKGGC 258

Query: 258 MDENHDVLYNLLNLAKENNGSMFDVYLIKHLILLVL------TTSTVEWAMAELLRNPKT 317
            DE +D L  LL + ++N+  + D+ +I HL+L +       T+STVEWAMA L  NP+ 
Sbjct: 259 FDEINDTLDALLKINQDNSEEL-DLSVIPHLLLDLFVGGSESTSSTVEWAMALLFSNPEK 318

Query: 318 LSKAKTELMEIVGKDNPVEESDFSRLPFLQAVVKETLRLHPPAPLLLPRKARQDTEIGGF 377
           + KAK EL  +VGK   V+E D  RLP+LQA +KET R+HPP P L+PRK   D ++ GF
Sbjct: 319 MKKAKEELETVVGKGIAVKEEDTGRLPYLQAAIKETFRMHPPTPFLIPRKTDSDVDLCGF 378

Query: 378 IIPKDAQVLVNAWDIQRDKNIWNDADLFKPERFLELENCIDDKGRNLELIPFGAGRRICP 437
            + K +QV+VNAW I RD ++W +AD F+PERFL +E  ID KGRN ELIPFGAGRRICP
Sbjct: 379 TVQKGSQVIVNAWAIGRDPSLWENADTFEPERFLGME--IDVKGRNFELIPFGAGRRICP 438

Query: 438 GLPLATRMVHWILASLIHSFDWKLEDGITPKTMNMDDKFGLTLAMAHPLKAIP 469
           GLP+A RM+  ++A+LI+ F+W+LE G  P+T++M DK G TL  AHP + IP
Sbjct: 439 GLPIAMRMLTLMVANLINCFEWRLEGGAAPETLDMSDKIGFTLQRAHPFRVIP 488

BLAST of HG10009221 vs. ExPASy Swiss-Prot
Match: W8JMV1 (Cytochrome P450 76T24 OS=Catharanthus roseus OX=4058 GN=CYP76T24 PE=2 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 1.3e-116
Identity = 213/469 (45.42%), Postives = 309/469 (65.88%), Query Frame = 0

Query: 24  SKLPPGPKGHLVIGNLMEIGEKPHQSLANLAKSHGPIMSLKLGQMTSIVISSAAMAKEVL 83
           ++LPPGP    VIGN+ ++G+ P+QSL  LAK++GP+MSLKLG  T++V+SS A+A+EVL
Sbjct: 32  ARLPPGPYPFPVIGNIFQLGQNPNQSLTKLAKTYGPLMSLKLGSNTTVVVSSPAVAREVL 91

Query: 84  QTHDQQLCDRTIPHSSTVYDHDKLGFPWLPVSDLWRT--------------LDSTEIIRR 143
           Q +DQ    R IPH++  + H K    WL VS LWR               LD++E +R+
Sbjct: 92  QKNDQVFSSRIIPHAAEAHAHHKYSMVWLQVSGLWRNLRKISKEHMFAAQRLDASEGLRQ 151

Query: 144 KQIQQLLTYIRESAVKGEVVDIGRGVFVATLNMLSNTIFSVDLANLNSEWAKDFKETVWG 203
           +++Q+L  Y+   +   + V+ G+  F  +LN +S+T FSVD A   S+ +++FK+ VW 
Sbjct: 152 EKLQELHDYLVRCSTSNKAVNFGQTAFTTSLNFISSTFFSVDFAAYGSDSSQEFKDIVWR 211

Query: 204 IMEESGRMDIGDYFPVLKKMDIQGSRRPMMVYIKKFLDMIGEMIDKRLELQ---EMDENH 263
           I++     ++ DYFPVLK MD QG  +       K  D+   +I++RL ++   +  + +
Sbjct: 212 ILKSYSTPNVADYFPVLKFMDPQGILQKNTFLFSKMFDIFDNIINERLMMRGSLDTSKKN 271

Query: 264 DVLYNLLNLAKENNGSMFDVYLIKHLILLVL------TTSTVEWAMAELLRNPKTLSKAK 323
           D+L  LLN    NN S F +  +KH++L +       T++T+EWAMAELLRNP+ L + +
Sbjct: 272 DLLEALLN-HSANNESEFSLNELKHMLLDLFLGGTETTSTTLEWAMAELLRNPEKLERVR 331

Query: 324 TELMEIVGKDNPVEESDFSRLPFLQAVVKETLRLHPPAPLLLPRKARQDTEIGGFIIPKD 383
            EL +++G+   + ESD SRLP+LQA+VKET RLHP  PLL+P KA  D EI G+ +PK+
Sbjct: 332 AELHQVIGEKEIIHESDISRLPYLQAIVKETFRLHPIVPLLIPHKAEADVEINGYTVPKN 391

Query: 384 AQVLVNAWDIQRDKNIWNDADLFKPERFLELENCIDDKGRNLELIPFGAGRRICPGLPLA 443
           +Q+L+N W   RD   W D + F PERFL  E  ID KGR+ ELIPFGAGRRICPGLPLA
Sbjct: 392 SQILINVWASGRDSGTWLDPETFSPERFLHSE--IDMKGRHFELIPFGAGRRICPGLPLA 451

Query: 444 TRMVHWILASLIHSFDWKLEDGITPKTMNMDDKFGLTLAMAHPLKAIPI 470
            R +H +LA+ IH+FDWKL+DG+ P+ ++M++K+GLTL +A PL  IP+
Sbjct: 452 YRTLHTMLATCIHNFDWKLKDGMKPEDIDMEEKYGLTLQLAVPLNVIPV 497

BLAST of HG10009221 vs. ExPASy TrEMBL
Match: A0A0A0KKE8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157290 PE=3 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 5.5e-214
Identity = 375/477 (78.62%), Postives = 416/477 (87.21%), Query Frame = 0

Query: 15  YKKSTKRSHSKLPPGPKGHLVIGNLMEIGEKPHQSLANLAKSHGPIMSLKLGQMTSIVIS 74
           +  STKR+HSKLPPGPKG+LVIGNLM+IG+KPHQSLANLAKSHGPIMSLKLGQMTSIVIS
Sbjct: 24  FNSSTKRTHSKLPPGPKGYLVIGNLMDIGDKPHQSLANLAKSHGPIMSLKLGQMTSIVIS 83

Query: 75  SAAMAKEVLQTHDQQLCDRTIPHSSTVYDHDKLGFPWLPVSDLWRT-------------- 134
           SAAMAKEVLQTHDQQLCDRTIP+SSTVYDHDKLGF WLPV D+WRT              
Sbjct: 84  SAAMAKEVLQTHDQQLCDRTIPYSSTVYDHDKLGFVWLPVCDVWRTLRKVCNNHMFSHKI 143

Query: 135 LDSTEIIRRKQIQQLLTYIRESAVKGEVVDIGRGVFVATLNMLSNTIFSVDLANLNSEWA 194
           LDST+IIR+KQIQ+LL  +RE A+KGE VDIG+  FV  LNMLSN +FSVDLA+ NSEWA
Sbjct: 144 LDSTKIIRQKQIQRLLDNVRERALKGEAVDIGKAAFVTILNMLSNMVFSVDLADPNSEWA 203

Query: 195 KDFKETVWGIMEESGRMDIGDYFPVLKKMDIQGSRRPMMVYIKKFLDMIGEMIDKRLELQ 254
           KD KETVWGIMEESG+ DIGDYFP+ K MDI+GSRR MMVYIKKFLDMIG+MI+KR+EL+
Sbjct: 204 KDLKETVWGIMEESGKPDIGDYFPLFKTMDIKGSRRRMMVYIKKFLDMIGDMIEKRMELE 263

Query: 255 EMDENHDVLYNLLNLAKENNGSMFDVYLIKHLILLVL------TTSTVEWAMAELLRNPK 314
            ++ENHD+LYNLLNLAKEN+ SMFDVYLIKHLIL++L      TTS VEWAMAELL+NP+
Sbjct: 264 GVEENHDMLYNLLNLAKENDDSMFDVYLIKHLILVLLPAGTDTTTSMVEWAMAELLKNPE 323

Query: 315 TLSKAKTELMEIVGKDNPVEESDFSRLPFLQAVVKETLRLHPPAPLLLPRKARQDTEIGG 374
            LSKA+ ELME+VGK+ P+EESD  +LPFLQA+ KETLRLHPP PLLLPRKARQDTEIGG
Sbjct: 324 ALSKARIELMEVVGKNRPIEESDILKLPFLQAIAKETLRLHPPVPLLLPRKARQDTEIGG 383

Query: 375 FIIPKDAQVLVNAWDIQRDKNIWNDADLFKPERFLELENCIDDKGRNLELIPFGAGRRIC 434
           F+IPKDAQV+VNAW IQRDKNIW D +LFKPERFLEL   ID KGRN+ELIPFGAGRRIC
Sbjct: 384 FVIPKDAQVIVNAWYIQRDKNIWEDGELFKPERFLELSE-IDYKGRNMELIPFGAGRRIC 443

Query: 435 PGLPLATRMVHWILASLIHSFDWKLEDGITPKTMNMDDKFGLTLAMAHPLKAIPIIV 472
           PGLPLA RM HWILASLIHSFDWKLEDGITPK MNMD+K GLTL MAHPLKAIPIIV
Sbjct: 444 PGLPLANRMGHWILASLIHSFDWKLEDGITPKNMNMDEKVGLTLVMAHPLKAIPIIV 499

BLAST of HG10009221 vs. ExPASy TrEMBL
Match: A0A1S3ATX6 (geraniol 8-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103482682 PE=3 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 8.8e-212
Identity = 371/477 (77.78%), Postives = 415/477 (87.00%), Query Frame = 0

Query: 15  YKKSTKRSHSKLPPGPKGHLVIGNLMEIGEKPHQSLANLAKSHGPIMSLKLGQMTSIVIS 74
           +  S KR+HSKLPPGPKG+LVIGNLM+IG+KPHQSLANLAKSHGPIMSLKLGQMTSIVIS
Sbjct: 23  FNSSNKRTHSKLPPGPKGYLVIGNLMDIGDKPHQSLANLAKSHGPIMSLKLGQMTSIVIS 82

Query: 75  SAAMAKEVLQTHDQQLCDRTIPHSSTVYDHDKLGFPWLPVSDLWRT-------------- 134
           SAAMAKEVLQTHDQQLCDRTIP SS VYDHDKLGFPWLPVSD+WRT              
Sbjct: 83  SAAMAKEVLQTHDQQLCDRTIPCSSAVYDHDKLGFPWLPVSDVWRTLRKVCNNHMFSQKI 142

Query: 135 LDSTEIIRRKQIQQLLTYIRESAVKGEVVDIGRGVFVATLNMLSNTIFSVDLANLNSEWA 194
           LDSTEIIRRKQIQ+LL  +RE A+KGE VDI +  F   LNMLSN IFSVDLA+ NSEWA
Sbjct: 143 LDSTEIIRRKQIQRLLDNVREKALKGEAVDIEKAAFGTILNMLSNMIFSVDLADPNSEWA 202

Query: 195 KDFKETVWGIMEESGRMDIGDYFPVLKKMDIQGSRRPMMVYIKKFLDMIGEMIDKRLELQ 254
           K+ KETVWGIMEESG+ DIGDYFP+LKKMDI+GSRR MMVYIKKFLDMIG+M++KR+EL+
Sbjct: 203 KELKETVWGIMEESGKPDIGDYFPLLKKMDIKGSRRRMMVYIKKFLDMIGDMMEKRMELE 262

Query: 255 EMDENHDVLYNLLNLAKENNGSMFDVYLIKHLILLVL------TTSTVEWAMAELLRNPK 314
           +M+ENHD+LYNLLNLA EN+GS+FD+YLIKHLIL++L      TTS VEWAMAELL+NP+
Sbjct: 263 DMEENHDMLYNLLNLATENDGSIFDIYLIKHLILVLLPAGSDTTTSMVEWAMAELLKNPE 322

Query: 315 TLSKAKTELMEIVGKDNPVEESDFSRLPFLQAVVKETLRLHPPAPLLLPRKARQDTEIGG 374
            LSKA+ ELME+VGK+ P+EESD S+LPFLQA+ KETLRLHPP PLLLPRKAR+DTEIGG
Sbjct: 323 ALSKARRELMEVVGKNRPIEESDLSKLPFLQAIAKETLRLHPPVPLLLPRKAREDTEIGG 382

Query: 375 FIIPKDAQVLVNAWDIQRDKNIWNDADLFKPERFLELENCIDDKGRNLELIPFGAGRRIC 434
           FI+PKDAQV+VNAW IQRD++IW D +LFKPERFLEL   ID KGRN+ELIPFGAGRRIC
Sbjct: 383 FIVPKDAQVIVNAWYIQRDRDIWEDGELFKPERFLELSE-IDYKGRNMELIPFGAGRRIC 442

Query: 435 PGLPLATRMVHWILASLIHSFDWKLEDGITPKTMNMDDKFGLTLAMAHPLKAIPIIV 472
           PGLPLA RM  WIL SLIHSFDWKLEDGITPK MNMD+K GLTL MAHPLKAIPIIV
Sbjct: 443 PGLPLANRMGLWILGSLIHSFDWKLEDGITPKNMNMDEKVGLTLVMAHPLKAIPIIV 498

BLAST of HG10009221 vs. ExPASy TrEMBL
Match: A0A5A7THP5 (Geraniol 8-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003300 PE=3 SV=1)

HSP 1 Score: 743.8 bits (1919), Expect = 4.4e-211
Identity = 369/477 (77.36%), Postives = 416/477 (87.21%), Query Frame = 0

Query: 15  YKKSTKRSHSKLPPGPKGHLVIGNLMEIGEKPHQSLANLAKSHGPIMSLKLGQMTSIVIS 74
           +  S KR+HSKLPPGPKG+LVIGNLM+IG+KPHQSLANLAKSHGPIMSLKLGQMTSIVIS
Sbjct: 23  FNSSNKRTHSKLPPGPKGYLVIGNLMDIGDKPHQSLANLAKSHGPIMSLKLGQMTSIVIS 82

Query: 75  SAAMAKEVLQTHDQQLCDRTIPHSSTVYDHDKLGFPWLPVSDLWRT-------------- 134
           SAAMAKEVLQTHDQQLCDRTIP SS VYDHDKLGFPWLPVSD+WRT              
Sbjct: 83  SAAMAKEVLQTHDQQLCDRTIPCSSAVYDHDKLGFPWLPVSDVWRTLRKVCNNHMFSQKI 142

Query: 135 LDSTEIIRRKQIQQLLTYIRESAVKGEVVDIGRGVFVATLNMLSNTIFSVDLANLNSEWA 194
           LDSTEIIRRKQIQ+L+  +RE A++GE VDI +  F   LNMLSN IFSVDLA+ NSEWA
Sbjct: 143 LDSTEIIRRKQIQRLVDNVREKALEGEAVDIEKAAFGTILNMLSNMIFSVDLADPNSEWA 202

Query: 195 KDFKETVWGIMEESGRMDIGDYFPVLKKMDIQGSRRPMMVYIKKFLDMIGEMIDKRLELQ 254
           K+ KETVWGIMEESG+ DIGDYFP+LKKMDI+GSRR MMVYIKKFLDMIG+M++KR+EL+
Sbjct: 203 KELKETVWGIMEESGKPDIGDYFPLLKKMDIKGSRRRMMVYIKKFLDMIGDMMEKRMELE 262

Query: 255 EMDENHDVLYNLLNLAKENNGSMFDVYLIKHLILLVL------TTSTVEWAMAELLRNPK 314
           +M+ENHD+LYNLLNLA EN+GS+FD+YLIKHLIL++L      TTS VEWAMAELL+NP+
Sbjct: 263 DMEENHDMLYNLLNLATENDGSIFDIYLIKHLILVLLPAGSDTTTSMVEWAMAELLKNPE 322

Query: 315 TLSKAKTELMEIVGKDNPVEESDFSRLPFLQAVVKETLRLHPPAPLLLPRKARQDTEIGG 374
            LSKA+ ELME++GK+ P+EESD S+LPFLQA+ KETLRLHPP PLLLPRKAR+DTEIGG
Sbjct: 323 ALSKARRELMEVMGKNRPIEESDLSKLPFLQAIAKETLRLHPPVPLLLPRKAREDTEIGG 382

Query: 375 FIIPKDAQVLVNAWDIQRDKNIWNDADLFKPERFLELENCIDDKGRNLELIPFGAGRRIC 434
           FI+PKDAQV+VNAW IQRDK+IW D++LFKPERFLEL   ID KGRN+ELIPFGAGRRIC
Sbjct: 383 FIVPKDAQVIVNAWYIQRDKDIWEDSELFKPERFLELSE-IDYKGRNMELIPFGAGRRIC 442

Query: 435 PGLPLATRMVHWILASLIHSFDWKLEDGITPKTMNMDDKFGLTLAMAHPLKAIPIIV 472
           PGLPLA RM  WIL SLIHSFDWKLEDGITPK MNMD+K GLTL MAHPLKAIPIIV
Sbjct: 443 PGLPLANRMGLWILGSLIHSFDWKLEDGITPKNMNMDEKVGLTLVMAHPLKAIPIIV 498

BLAST of HG10009221 vs. ExPASy TrEMBL
Match: A0A6J1DZB2 (geraniol 8-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111025978 PE=3 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 4.7e-189
Identity = 347/486 (71.40%), Postives = 396/486 (81.48%), Query Frame = 0

Query: 11  VFKSYKKSTKRSHSKLPPGPKGHLVIGNLMEIGEKPHQSLANLAKSHGPIMSLKLGQMTS 70
           +F S + S+++  +K PPGPKG+ +IGNL+E+G+KPHQSLA LA SHGPIM LKLGQMT+
Sbjct: 19  LFNSKRSSSRQWKNKRPPGPKGYPLIGNLLEMGDKPHQSLAILAASHGPIMRLKLGQMTT 78

Query: 71  IVISSAAMAKEVLQTHDQQLCDRTIPHSSTVYDHDKLGFPWLPVSDLWRT---------- 130
           IVISSAAMAK+VLQTHDQ L DRT+PHSSTVYDHDKLGFPWLPVSDLWRT          
Sbjct: 79  IVISSAAMAKQVLQTHDQALSDRTVPHSSTVYDHDKLGFPWLPVSDLWRTLRKVCNNHMF 138

Query: 131 ----LDSTEIIRRKQIQQLLTYIRESAVKGEVVDIGRGVFVATLNMLSNTIFSVDLANLN 190
               LDS E IRR  + +LL  IR SA  GE VDI R VF A  NMLSNTIFSVDLA+ N
Sbjct: 139 SHNALDSHEPIRRNNVGRLLADIRRSAHNGESVDIERAVFGAAFNMLSNTIFSVDLADPN 198

Query: 191 SEWAKDFKETVWGIMEESGRMDIGDYFPVLKKMDIQGSRRPMMVYIKKFLDMIGEMIDKR 250
           SEWAK+FKETVWGI+EESG+ D+GDYFPVLK MD++GSRR MMVYIKKFLDMI EMI++R
Sbjct: 199 SEWAKEFKETVWGILEESGKFDVGDYFPVLKWMDLRGSRRRMMVYIKKFLDMIVEMIEER 258

Query: 251 LELQE----MDENH-DVLYNLLNLAKENNGSMFDVYLIKHLILLVL------TTSTVEWA 310
              QE     D  H D+ +NLLNL KEN    FDVYLIKHLIL++       TTSTV+WA
Sbjct: 259 FRRQEFAGGFDGGHRDMFHNLLNLPKENTYPNFDVYLIKHLILVLFPAGTDTTTSTVQWA 318

Query: 311 MAELLRNPKTLSKAKTELMEIVGKDNPVEESDFSRLPFLQAVVKETLRLHPPAPLLLPRK 370
           MAELLRN K L KA+TEL E++GKD P++ESDFSRLPFL+A+VKETLRLHPPAPLLLPRK
Sbjct: 319 MAELLRNRKILLKAQTELREVIGKD-PLKESDFSRLPFLRAIVKETLRLHPPAPLLLPRK 378

Query: 371 ARQDTEIGGFIIPKDAQVLVNAWDIQRDKNIWNDADLFKPERFLELENCIDDKGRNLELI 430
           AR+DT+I GF+IPKDAQV+VNAW+I RDK+IW DA  FKPERFL  E  ID KGRN ELI
Sbjct: 379 AREDTKIEGFLIPKDAQVIVNAWEIGRDKSIWEDASSFKPERFLGSE--IDFKGRNFELI 438

Query: 431 PFGAGRRICPGLPLATRMVHWILASLIHSFDWKLEDGITPKTMNMDDKFGLTLAMAHPLK 472
           PFGAGRRICPGLPLATRMVHWIL SLIHSFDWKLEDGITP+T+NMD+K GLT+ MAHPL+
Sbjct: 439 PFGAGRRICPGLPLATRMVHWILGSLIHSFDWKLEDGITPETINMDEKVGLTVVMAHPLR 498

BLAST of HG10009221 vs. ExPASy TrEMBL
Match: A0A6J1EM55 (geraniol 8-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111435787 PE=3 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 2.1e-165
Identity = 299/472 (63.35%), Postives = 367/472 (77.75%), Query Frame = 0

Query: 20  KRSHSKLPPGPKGHLVIGNLMEIGEKPHQSLANLAKSHGPIMSLKLGQMTSIVISSAAMA 79
           K +  +LPPGPKG+ +IGN+MEIG  PHQSLA LA S+GPIM+LKLGQMTSIVISS +MA
Sbjct: 37  KINPKRLPPGPKGYFLIGNMMEIGHNPHQSLAKLADSYGPIMTLKLGQMTSIVISSPSMA 96

Query: 80  KEVLQTHDQQLCDRTIPHSSTVYDHDKLGFPWLPVSDLWR--------------TLDSTE 139
           K+VLQ+HDQ L DR  P ++T+YDH K+  PWLPV DLWR              TL    
Sbjct: 97  KQVLQSHDQVLSDRMYPDATTIYDHHKVALPWLPVCDLWRTLRKLCNNHMFSENTLHMNY 156

Query: 140 IIRRKQIQQLLTYIRESAVKGEVVDIGRGVFVATLNMLSNTIFSVDLANLNS---EWAKD 199
            IR K+IQ+LL  ++ESA KGE VDI R VFV TL+MLSN IFSVDL++  S   E  ++
Sbjct: 157 TIRLKKIQRLLADVQESATKGEAVDIERVVFVTTLDMLSNMIFSVDLSDEYSNIYEKGQE 216

Query: 200 FKETVWGIMEESGRMDIGDYFPVLKKMDIQGSRRPMMVYIKKFLDMIGEMIDKRLELQEM 259
           FKE VWGIMEE+GR ++GD FP+LKKMD+QG RR MM ++ KFLD+I +MI KR++   +
Sbjct: 217 FKEAVWGIMEEAGRSNVGDLFPMLKKMDLQGCRRRMMAHMNKFLDIIDDMIGKRMKEPNL 276

Query: 260 DENHDVLYNLLNLAKENNGSMFDVYLIKHLILLVL------TTSTVEWAMAELLRNPKTL 319
            E +D+L+NLLNLAKE+  + F ++LIKHLIL++       TTSTVEWAMAELLR PK L
Sbjct: 277 GEENDMLHNLLNLAKESEDAKFHLHLIKHLILVLFPAGTDTTTSTVEWAMAELLRKPKVL 336

Query: 320 SKAKTELMEIVGKDNPVEESDFSRLPFLQAVVKETLRLHPPAPLLLPRKARQDTEIGGFI 379
           SKAK EL E++G+   V+ESD+ RLPFLQ ++KETLRLHPPAPLLLP KAR+D EIGGF+
Sbjct: 337 SKAKAELKEVIGEGTAVDESDWVRLPFLQQIIKETLRLHPPAPLLLPHKAREDAEIGGFV 396

Query: 380 IPKDAQVLVNAWDIQRDKNIWNDADLFKPERFLELENCIDDKGRNLELIPFGAGRRICPG 439
           +PK+A+V+VNAW I RDK +W DAD FKPERFL  +  +D KGR+ ELIPFG+GRRICPG
Sbjct: 397 VPKNARVIVNAWKIGRDKGVWEDADSFKPERFLGSD--VDYKGRHFELIPFGSGRRICPG 456

Query: 440 LPLATRMVHWILASLIHSFDWKLEDGITPKTMNMDDKFGLTLAMAHPLKAIP 469
           L LAT+MVHWILASLIHSF W+LEDGI P+TMNM++K GLTLAMA PL+AIP
Sbjct: 457 LSLATKMVHWILASLIHSFHWRLEDGIQPETMNMEEKTGLTLAMARPLRAIP 506

BLAST of HG10009221 vs. TAIR 10
Match: AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )

HSP 1 Score: 396.7 bits (1018), Expect = 2.5e-110
Identity = 211/481 (43.87%), Postives = 315/481 (65.49%), Query Frame = 0

Query: 16  KKSTKRSHSKLPPGPKGHLVIGNLMEIGEKPHQSLANLAKSHGPIMSLKLGQMTSIVISS 75
           ++S+ R+ + LPPGP    +IGN+ ++G+ PH S A+LAK +GPIMSLK G + S+VI+S
Sbjct: 29  RRSSGRA-ATLPPGPPRLPIIGNIHQVGKNPHSSFADLAKIYGPIMSLKFGCLNSVVITS 88

Query: 76  AAMAKEVLQTHDQQLCDRTIPHSSTVYDHDKLGFPWLPVSDL-WRTL------------- 135
              A+EVL+THDQ L  R    S   + H+++   WLP S   WR L             
Sbjct: 89  PEAAREVLRTHDQILSGRKSNDSIRCFGHEEVSVIWLPPSSARWRMLRKLSVTLMFSPQR 148

Query: 136 -DSTEIIRRKQIQQLLTYIRESAVKGEVVDIGRGVFVATLNMLSNTIFSVDLANLNSEWA 195
            ++T+ +R K++Q+L++++ ES+ + E VDI R  +   LN++SN +FSVDL + +S+ +
Sbjct: 149 TEATKALRMKKVQELVSFMNESSERKEAVDISRASYTTVLNIISNILFSVDLGSYDSKKS 208

Query: 196 KDFKETVWGIMEESGRMDIGDYFPVLKKMDIQGSRRPMMVYIKKFLDMIGEMIDKRLELQ 255
            +F++TV G ME +G+ D  +YFP +  +D+QG+R+ M    ++   +    +D ++  +
Sbjct: 209 NEFQDTVIGAMEAAGKPDAANYFPFMGFLDLQGNRKAMRGLTERLFRVFRGFMDAKIAEK 268

Query: 256 EMD------ENHDVLYNLLNLAKENNGSMFDVYLIKHLILLVLT------TSTVEWAMAE 315
            +        N D L +LL +  E + +  D   I+HL+L + T      +ST+EWAMAE
Sbjct: 269 SLGNYSKDVSNRDFLDSLL-ILNEGDEAELDNNDIEHLLLDMFTAGTDTSSSTLEWAMAE 328

Query: 316 LLRNPKTLSKAKTELMEIVGKDNPVEESDFSRLPFLQAVVKETLRLHPPAPLLLPRKARQ 375
           LLRNPKT+ KA+ E+  ++G+++ V+ESD S LP+LQAVVKET RLHP APLL+PRKA  
Sbjct: 329 LLRNPKTMVKAQAEMDRVLGQNSVVQESDISGLPYLQAVVKETFRLHPAAPLLVPRKAES 388

Query: 376 DTEIGGFIIPKDAQVLVNAWDIQRDKNIWNDADLFKPERFLELENCIDDKGRNLELIPFG 435
           D E+ GF++PKD QVLVN W I RD ++W +   F+PERF+  +  ID KGR+ EL PFG
Sbjct: 389 DVEVLGFMVPKDTQVLVNVWAIGRDPSVWENPSQFEPERFMGKD--IDVKGRDYELTPFG 448

Query: 436 AGRRICPGLPLATRMVHWILASLIHSFDWKLEDGITPKTMNMDDKFGLTLAMAHPLKAIP 470
            GRRICPGLPLA + V  +LASL++SFDWKL +G+  + ++MD+ FG+TL   + L AIP
Sbjct: 449 GGRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVSEDLDMDETFGITLHRTNTLYAIP 505

BLAST of HG10009221 vs. TAIR 10
Match: AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )

HSP 1 Score: 393.3 bits (1009), Expect = 2.8e-109
Identity = 211/486 (43.42%), Postives = 309/486 (63.58%), Query Frame = 0

Query: 12  FKSYKKSTKRSHSKLPPGPKGHLVIGNLMEIGEKPHQSLANLAKSHGPIMSLKLGQMTSI 71
           F + +  + R     PPGP    +IGN+  +G  PH S A+L+K++GPIMSLK G + ++
Sbjct: 24  FTTTRPRSSRKVVPSPPGPPRLPIIGNIHLVGRNPHHSFADLSKTYGPIMSLKFGSLNTV 83

Query: 72  VISSAAMAKEVLQTHDQQLCDRTIPHSSTVYDHDKLGFPWL-PVSDLWRTL--------- 131
           V++S   A+EVL+T+DQ L  RT  +S    +HDK+   WL P S  WR L         
Sbjct: 84  VVTSPEAAREVLRTYDQILSSRTPTNSIRSINHDKVSVVWLPPSSSRWRLLRKLSATQLF 143

Query: 132 -----DSTEIIRRKQIQQLLTYIRESAVKGEVVDIGRGVFVATLNMLSNTIFSVDLANLN 191
                ++T+ +R  ++++L++++ ES+ + E VDI R  F+  LN++SN +FSVDL N +
Sbjct: 144 SPQRIEATKTLRENKVKELVSFMSESSEREEAVDISRATFITALNIISNILFSVDLGNYD 203

Query: 192 SEWAKDFKETVWGIMEESGRMDIGDYFPVLKKMDIQGSRRPMMVYIKKFLDMIGEMIDKR 251
           S  +  F++TV G+ME  G  D  ++FP L  +D+QG+R+ +    ++   +    ID +
Sbjct: 204 SNKSGVFQDTVIGVMEAVGNPDAANFFPFLGFLDLQGNRKTLKACSERLFKVFRGFIDAK 263

Query: 252 LELQEMDENH-------DVLYNLLNLAKENNGSMFDVYLIKHLILLVL------TTSTVE 311
           L  + + + +       D +  LL+L  E + +  +   I HL+L +        +STVE
Sbjct: 264 LAEKSLRDTNSKDVRERDFVDVLLDLT-EGDEAELNTNDIVHLLLDLFGAGTDTNSSTVE 323

Query: 312 WAMAELLRNPKTLSKAKTELMEIVGKDNPVEESDFSRLPFLQAVVKETLRLHPPAPLLLP 371
           WAMAELLRNP+T+ KA+ E+  ++G+   VEESD S LP+LQAVVKET RLHP APLL+P
Sbjct: 324 WAMAELLRNPETMVKAQAEIDCVIGQKGVVEESDISALPYLQAVVKETFRLHPAAPLLVP 383

Query: 372 RKARQDTEIGGFIIPKDAQVLVNAWDIQRDKNIWNDADLFKPERFLELENCIDDKGRNLE 431
           RKA  D E+ GF++PKD QV VN W I RD N+W ++  FKPERFL  +  ID +GR+ E
Sbjct: 384 RKAESDVEVLGFMVPKDTQVFVNVWAIGRDPNVWENSSRFKPERFLGKD--IDLRGRDYE 443

Query: 432 LIPFGAGRRICPGLPLATRMVHWILASLIHSFDWKLEDGITPKTMNMDDKFGLTLAMAHP 470
           L PFGAGRRICPGLPLA + V  +LASL++SFDWKL +G+  + ++MD+ FGLTL   +P
Sbjct: 444 LTPFGAGRRICPGLPLAVKTVPLMLASLLYSFDWKLPNGVGSEDLDMDETFGLTLHKTNP 503

BLAST of HG10009221 vs. TAIR 10
Match: AT2G45560.1 (cytochrome P450, family 76, subfamily C, polypeptide 1 )

HSP 1 Score: 380.6 bits (976), Expect = 1.9e-105
Identity = 203/477 (42.56%), Postives = 309/477 (64.78%), Query Frame = 0

Query: 21  RSHSKLPPGPKGHLVIGNLMEIGEKPHQSLANLAKSHGPIMSLKLGQMTSIVISSAAMAK 80
           R  + LPPGP    +IGN+  +G+ PH+S A L+K++GP+MSLKLG + ++VI+S   A+
Sbjct: 33  RGATALPPGPPRLPIIGNIHLVGKHPHRSFAELSKTYGPVMSLKLGSLNTVVIASPEAAR 92

Query: 81  EVLQTHDQQLCDRTIPHSSTVYDHDKLGFPWLPVSDL-WRTL--------------DSTE 140
           EVL+THDQ L  R+  ++    +H      WLP S   WR L              ++T+
Sbjct: 93  EVLRTHDQILSARSPTNAVRSINHQDASLVWLPSSSARWRLLRRLSVTQLLSPQRIEATK 152

Query: 141 IIRRKQIQQLLTYIRESAVKGEVVDIGRGVFVATLNMLSNTIFSVDLANLNSEWA-KDFK 200
            +R  ++++L+++I ES+ + E VDI R  F+ TLN++SN +FSVDL + N++ +    +
Sbjct: 153 ALRMNKVKELVSFISESSDREESVDISRVAFITTLNIISNILFSVDLGSYNAKASINGVQ 212

Query: 201 ETVWGIMEESGRMDIGDYFPVLKKMDIQGSRRPMMVYIKKFLDMIGEMIDKRLELQEMDE 260
           +TV  +M+ +G  D  +YFP L+ +D+QG+ +   V  ++ + +    ID ++  +    
Sbjct: 213 DTVISVMDAAGTPDAANYFPFLRFLDLQGNVKTFKVCTERLVRVFRGFIDAKIAEKSSQN 272

Query: 261 N------HDVLYNLLNLAKENNGSMFDVYLIKHLILLVLT------TSTVEWAMAELLRN 320
           N      +D + NLL+   + + S   +  I+HL+L + T      +ST+EWAM ELL+N
Sbjct: 273 NPKDVSKNDFVDNLLDY--KGDESELSISDIEHLLLDMFTAGTDTSSSTLEWAMTELLKN 332

Query: 321 PKTLSKAKTELMEIVGKDNPVEESDFSRLPFLQAVVKETLRLHPPAPLLLPRKARQDTEI 380
           PKT++KA+ E+  ++G++  VEESD S+LP+LQAVVKET RLH P PLL+PRKA  D EI
Sbjct: 333 PKTMAKAQAEIDCVIGQNGIVEESDISKLPYLQAVVKETFRLHTPVPLLIPRKAESDAEI 392

Query: 381 GGFIIPKDAQVLVNAWDIQRDKNIWNDADLFKPERFLELENCIDDKGRNLELIPFGAGRR 440
            GF++ KD QVLVN W I RD ++W++   F+PERFL  +  +D +GR+ EL PFGAGRR
Sbjct: 393 LGFMVLKDTQVLVNVWAIGRDPSVWDNPSQFEPERFLGKD--MDVRGRDYELTPFGAGRR 452

Query: 441 ICPGLPLATRMVHWILASLIHSFDWKLEDGITPKTMNMDDKFGLTLAMAHPLKAIPI 470
           ICPG+PLA + V  +LASL++SFDWKL  G+  + ++MD+ FGLTL   +PL A+P+
Sbjct: 453 ICPGMPLAMKTVSLMLASLLYSFDWKLPKGVLSEDLDMDETFGLTLHKTNPLHAVPV 505

BLAST of HG10009221 vs. TAIR 10
Match: AT2G45580.1 (cytochrome P450, family 76, subfamily C, polypeptide 3 )

HSP 1 Score: 377.5 bits (968), Expect = 1.6e-104
Identity = 198/487 (40.66%), Postives = 310/487 (63.66%), Query Frame = 0

Query: 12  FKSYKKSTKRSHSKLPPGPKGHLVIGNLMEIGEKPHQSLANLAKSHGPIMSLKLGQMTSI 71
           F + K     S   LPPGP    ++GN+ ++G  PH+SLA  +K++GPIMSLKLG++T++
Sbjct: 24  FATAKTRRSSSTGTLPPGPPILPLVGNIFQLGFNPHRSLAAFSKTYGPIMSLKLGRLTAV 83

Query: 72  VISSAAMAKEVLQTHDQQLCDRTIPHSSTVYDHDKLGFPWLPVSDLWR------------ 131
           VISS   AKE L+THD  +  RT   +   +DH K    W+P S  WR            
Sbjct: 84  VISSPEAAKEALRTHDHVMSARTFNDALRAFDHHKHSIVWIPPSARWRFLKKTITKYLLS 143

Query: 132 --TLDSTEIIRRKQIQQLLTYIRESAVKGEVVDIGRGVFVATLNMLSNTIFSVDLANLNS 191
              LD+ + +R +++++L++ + E   +GE +D+ R  FV + N++SN +FSVDLA  +S
Sbjct: 144 PQNLDAIQSLRMRKVEELVSLVNEFRERGEAIDLARASFVTSFNIISNALFSVDLATYDS 203

Query: 192 EWAK-DFKETVWGIMEESGRMDIGDYFPVLKKMDIQGSRRPMMVYIKKFLDMIGEMIDKR 251
             +  +F  TV  + + +G  ++GDYF  ++ +D+QG+R+  ++ I+K   +  E ID R
Sbjct: 204 NSSSYEFHNTVVHLTDIAGIPNVGDYFQYMRFLDLQGTRKKAVLCIEKLFRVFQEFIDAR 263

Query: 252 LELQ--------EMDENHDVLYNLLNLAKENNGSMFDVYLIKHLILLVL------TTSTV 311
           L  +        +   + D+L +LL+L ++N   +  +  +KHL+L V        +ST+
Sbjct: 264 LAKRFSRTEKEPKEASSIDMLDSLLDLTQQNEAEL-TMNDLKHLLLDVFVAGTDTNSSTM 323

Query: 312 EWAMAELLRNPKTLSKAKTELMEIVGKDNPVEESDFSRLPFLQAVVKETLRLHPPAPLLL 371
           EWAM EL R+ + + KA++E+ +++G++  V+ESD   LP+LQA+VKETLRLHP AP L+
Sbjct: 324 EWAMTELFRSTEKMVKAQSEIRQVIGQNGFVQESDIPSLPYLQAIVKETLRLHPAAP-LI 383

Query: 372 PRKARQDTEIGGFIIPKDAQVLVNAWDIQRDKNIWNDADLFKPERFLELENCIDDKGRNL 431
           PRK+  D +I GF++PK+ QV+VN W I RD ++W +   F+PERFL  E   D KGR+ 
Sbjct: 384 PRKSESDVQIMGFLVPKNTQVVVNVWAIGRDASVWENPMKFEPERFLLRET--DVKGRDF 443

Query: 432 ELIPFGAGRRICPGLPLATRMVHWILASLIHSFDWKLEDGITPKTMNMDDKFGLTLAMAH 470
           ELIPFG+GRR+CPG+ +A + +H +LASL++SFDWKL++G+ P  ++M + FGLTL  A 
Sbjct: 444 ELIPFGSGRRMCPGISMALKTMHMVLASLLYSFDWKLQNGVVPGNIDMSETFGLTLHKAK 503

BLAST of HG10009221 vs. TAIR 10
Match: AT3G61040.1 (cytochrome P450, family 76, subfamily C, polypeptide 7 )

HSP 1 Score: 368.6 bits (945), Expect = 7.4e-102
Identity = 198/468 (42.31%), Postives = 297/468 (63.46%), Query Frame = 0

Query: 24  SKLPPGPKGHLVIGNLMEIGEKPHQSLANLAKSHGPIMSLKLGQMTSIVISSAAMAKEVL 83
           +K PPGP    ++ N+++  EKPH+SLA+L++ +G +MS KLG +T++VISS   AKEVL
Sbjct: 31  AKNPPGPSKLSLLRNILQTVEKPHRSLADLSRIYGSVMSFKLGCLTTVVISSPETAKEVL 90

Query: 84  QTHDQQLCDRTIPHSSTVYDHDKLGFPWLPVSDLWR--------------TLDSTEIIRR 143
           +THD  L  R          H +L   W+P    WR               L++T  IR 
Sbjct: 91  KTHDHVLSYRVSSDPVRAAGHHELSLLWIPPLARWRFLRKITRNQLFSTQRLEATSAIRT 150

Query: 144 KQIQQLLTYIRESAVKGEVVDIGRGVFVATLNMLSNTIFSVDLANL-NSEWAKDFKETVW 203
           +++Q+L+ ++ +   + E V+I R  F+ +LN++SN +FS +LAN  +S+   DF+  V 
Sbjct: 151 RKVQELMNFVNKCCERREAVNISRASFITSLNIISNALFSTNLANFDDSKTFHDFQNVVI 210

Query: 204 GIMEESGRMDIGDYFPVLKKMDIQGSRRPMMVYIKKFLDMIGEMIDKRLELQEMDENHDV 263
            +ME SG+ ++ D+FP L  +D+QG+R+   + + K   +    ID +      + N+D+
Sbjct: 211 RMMEISGKPNLADFFPFLGFLDLQGARKEARLLMHKLFRVFQGFIDTKRSSTSRN-NNDM 270

Query: 264 LYNLLNLAKENNGSMFDVYLIKHLIL------LVLTTSTVEWAMAELLRNPKTLSKAKTE 323
           L +LL++A +   S  D   IKHL+L      +  ++S VEWAMAELLRNPK + K + E
Sbjct: 271 LDSLLDIAHKKE-SELDDNNIKHLLLDLFLAGVDTSSSAVEWAMAELLRNPKMIVKVQEE 330

Query: 324 LMEIVGKDNPVEESDFSRLPFLQAVVKETLRLHPPAPLLLPRKAR-QDTEIGGFIIPKDA 383
           + +++G    V++ D  +LP+LQAVVKE+LRLHPPAP L+PRK+   D +I  F+IPK+ 
Sbjct: 331 IRQVIGLKGTVQDLDIVKLPYLQAVVKESLRLHPPAPFLVPRKSESDDVQIFEFLIPKNT 390

Query: 384 QVLVNAWDIQRDKNIWNDADLFKPERFLELENCIDDKGRNLELIPFGAGRRICPGLPLAT 443
           QVLVN W I RD N+W +   F+PERF  L   ID KG + ELIPFGAGRRICPG+PLA 
Sbjct: 391 QVLVNVWAIGRDPNVWKNPTQFEPERF--LGRGIDVKGNHFELIPFGAGRRICPGMPLAF 450

Query: 444 RMVHWILASLIHSFDWKLEDGITPKTMNMDDKFGLTLAMAHPLKAIPI 470
           R++H +LASL++ FDW+ ++G+ P+ ++M++ FG TL  A PL  +PI
Sbjct: 451 RIMHLVLASLLYGFDWEYQNGVVPENVDMNEAFGATLHKAEPLCIVPI 494

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004147600.21.1e-21378.62geraniol 8-hydroxylase [Cucumis sativus] >KGN50170.1 hypothetical protein Csa_00... [more]
XP_008437176.11.8e-21177.78PREDICTED: geraniol 8-hydroxylase-like [Cucumis melo][more]
KAA0042823.19.1e-21177.36geraniol 8-hydroxylase-like [Cucumis melo var. makuwa][more]
XP_022159618.19.7e-18971.40geraniol 8-hydroxylase-like [Momordica charantia][more]
XP_038874677.11.8e-17165.26geraniol 8-hydroxylase-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
D1MI463.3e-12347.11Geraniol 8-hydroxylase OS=Swertia mussotii OX=137888 GN=CYP76B10 PE=1 SV=1[more]
Q8VWZ78.2e-12246.79Geraniol 8-hydroxylase OS=Catharanthus roseus OX=4058 GN=CYP76B6 PE=1 SV=1[more]
I3PFJ57.6e-12047.86Cytochrome P450 76AD1 OS=Beta vulgaris OX=161934 GN=CYP76AD1 PE=2 SV=1[more]
W0FKI04.2e-11846.51Drimenol monooxygenase OS=Persicaria hydropiper OX=46901 GN=CYP76AJ1 PE=1 SV=1[more]
W8JMV11.3e-11645.42Cytochrome P450 76T24 OS=Catharanthus roseus OX=4058 GN=CYP76T24 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KKE85.5e-21478.62Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157290 PE=3 SV=1[more]
A0A1S3ATX68.8e-21277.78geraniol 8-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103482682 PE=3 SV=1[more]
A0A5A7THP54.4e-21177.36Geraniol 8-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
A0A6J1DZB24.7e-18971.40geraniol 8-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111025978 PE=3 ... [more]
A0A6J1EM552.1e-16563.35geraniol 8-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111435787 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT2G45550.12.5e-11043.87cytochrome P450, family 76, subfamily C, polypeptide 4 [more]
AT2G45570.12.8e-10943.42cytochrome P450, family 76, subfamily C, polypeptide 2 [more]
AT2G45560.11.9e-10542.56cytochrome P450, family 76, subfamily C, polypeptide 1 [more]
AT2G45580.11.6e-10440.66cytochrome P450, family 76, subfamily C, polypeptide 3 [more]
AT3G61040.17.4e-10242.31cytochrome P450, family 76, subfamily C, polypeptide 7 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 273..290
score: 22.1
coord: 405..414
score: 60.9
coord: 326..337
score: 49.25
coord: 414..425
score: 32.6
IPR001128Cytochrome P450PFAMPF00067p450coord: 27..454
e-value: 9.5E-81
score: 271.9
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 78..99
score: 21.73
coord: 325..343
score: 41.95
coord: 54..73
score: 28.53
coord: 282..308
score: 28.24
coord: 404..414
score: 57.78
coord: 366..390
score: 34.36
coord: 414..437
score: 29.94
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 11..466
e-value: 4.5E-104
score: 350.7
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 27..467
NoneNo IPR availablePANTHERPTHR47950:SF4CYTOCHROME P450 FAMILY MONOOXYGENASEcoord: 12..470
NoneNo IPR availablePANTHERPTHR47950CYTOCHROME P450, FAMILY 76, SUBFAMILY C, POLYPEPTIDE 5-RELATEDcoord: 12..470
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 407..416

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10009221.1HG10009221.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen