HG10009218 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10009218 vs. NCBI nr
Match: XP_038906452.1 (subtilisin-like protease 4 [Benincasa hispida])

HSP 1 Score: 1122.1 bits (2901), Expect = 0.0e+00
Identity = 572/731 (78.25%), Postives = 629/731 (86.05%), Query Frame = 0

Query: 21  MKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLESWYTSFLPKTIETSNEQSRLLYSY 80
           MK+ +S TSN IKLQTYIVHV++ EL    D  DLESWYTSFLP+TI+TSNEQSRLLYS+
Sbjct: 1   MKSELSGTSNQIKLQTYIVHVKESELRILGDAIDLESWYTSFLPETIKTSNEQSRLLYSF 60

Query: 81  RHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNFG 140
           R+VMSGFSARLTEE VK+MEEK+GFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNFG
Sbjct: 61  RNVMSGFSARLTEE-VKAMEEKEGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNFG 120

Query: 141 KGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNLANTVLM 200
           KGVIIGVLDTGI+PDHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFN AN+ LM
Sbjct: 121 KGVIIGVLDTGISPDHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNRANSALM 180

Query: 201 GETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGIAPLAHVAMYKVCS-KGCQSSD 260
           GE+PIDENGHGTHTASTAAGTFVEGAEALGNARGKAVG+APLAH+AMYKVCS KGC SSD
Sbjct: 181 GESPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGMAPLAHLAMYKVCSPKGCSSSD 240

Query: 261 ILAGLDAAIDDGVDVLSISIGSGS------------------------------------ 320
           ILA LDAAI DGVDVLS+S+G+ S                                    
Sbjct: 241 ILAALDAAIHDGVDVLSLSLGAPSRPFYIDNIAIGAFAAIKMGIFVSCSAGNSGPSKSTL 300

Query: 321 ---------VGASTIDRTIAALAKLGNDEVHVGESLFQPRDFTSTFLPLVYAGNSGTKRS 380
                    VGASTIDRTI ALA+LGN EVHVGESLFQPRDF S+FLPLVYAG+SG K S
Sbjct: 301 ANEAPWILTVGASTIDRTIMALARLGNGEVHVGESLFQPRDFPSSFLPLVYAGSSGVKGS 360

Query: 381 EFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNAGGAAMILINQKPNGFTTSADAH 440
           EFCVQGSL NL+VKGK+VVCE GG IGR+ KG+VVKNAGGAAMI+INQK + F+T A+AH
Sbjct: 361 EFCVQGSLTNLNVKGKMVVCERGGGIGRIAKGMVVKNAGGAAMIIINQKADEFSTLAEAH 420

Query: 441 VLPATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGGSNTDFSPAMASFSSRGPCLSSH 500
           +LPAT VSYEAGLKIK+YI SSQNP ASISF+GT+IG   T FSPAMASFSSRGPCL S 
Sbjct: 421 ILPATHVSYEAGLKIKSYIRSSQNPMASISFEGTLIGSRATTFSPAMASFSSRGPCLPSP 480

Query: 501 GILKPDITGPGVNILAAWPFPIDSNKNTKSTFNVMSGTSMSCPHLSGIAALIKNVHPNWS 560
           GILKPDITGPGVNILAAWPF +D+N N KSTFNV+SGTSMSCPHLSGIAALIK+VHPNWS
Sbjct: 481 GILKPDITGPGVNILAAWPFSLDNNTNKKSTFNVISGTSMSCPHLSGIAALIKSVHPNWS 540

Query: 561 PAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFATGSGHVNPSKAADPGLVYDIQADDYV 620
           PAAIKSAIMTSADV+  QG+PIVDQDLKPANFFA GSGHVNPSKAADPGLVYDIQ DDY+
Sbjct: 541 PAAIKSAIMTSADVRNRQGRPIVDQDLKPANFFAMGSGHVNPSKAADPGLVYDIQPDDYI 600

Query: 621 RYLCGLYTDVQVSMIVRRQITCSTVSKIREGDLNYPSFSVTLGASQTFSRTVTNVGDANS 680
            YLCGLY+D QVS+IVRR++TCST+S+IREGDLNYPSF+VTLG SQ F+RTVTNVGDANS
Sbjct: 601 PYLCGLYSDAQVSIIVRRKVTCSTISRIREGDLNYPSFAVTLGPSQMFNRTVTNVGDANS 660

Query: 681 VYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVTFSVDALVKVTSEFSEGYLKWVSDKH 706
           +YSAIVEAP+GVSV+VTP+NLKFS + EK+TYSVTFS D LV+ TSEFSEGYL WVS+KH
Sbjct: 661 IYSAIVEAPIGVSVKVTPNNLKFSRVIEKVTYSVTFSRDDLVRTTSEFSEGYLIWVSNKH 720

BLAST of HG10009218 vs. NCBI nr
Match: XP_004147599.2 (subtilisin-like protease SBT1.7 [Cucumis sativus] >KAE8647878.1 hypothetical protein Csa_000520 [Cucumis sativus])

HSP 1 Score: 1095.9 bits (2833), Expect = 0.0e+00
Identity = 567/753 (75.30%), Postives = 628/753 (83.40%), Query Frame = 0

Query: 1   MVVLPLFCIFFLLNFHGNVAMKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLESWYT 60
           MV LP  CIFF LNFHG VAMKT +   SNT  LQTYIVHV+QPE+E   DT DL++WYT
Sbjct: 1   MVFLPFICIFFFLNFHGYVAMKTELWSASNT-NLQTYIVHVKQPEVEILGDTIDLQNWYT 60

Query: 61  SFLPKTIE-TSNEQSRLLYSYRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTT 120
           SFLP+TIE +SNEQSRLLYSYRHV+SGFSARLT+EQVK+MEEKDGFISA PET LNLHTT
Sbjct: 61  SFLPETIEASSNEQSRLLYSYRHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTT 120

Query: 121 HTPEYLGLNQQFGLWKDSNFGKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCEFGA 180
           HTPEYLGLNQ FGLWK+SNFGKGVIIGVLDTGI+P+HPSFND+GMP PPAKWKGRCEFGA
Sbjct: 121 HTPEYLGLNQHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGA 180

Query: 181 SICNNKLIGARTFNLANTVLMGETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGI 240
           SICNNKLIGARTFNLAN V +G++P DENGHGTHTASTAAGTFV+GAEALGNARGKAVG+
Sbjct: 181 SICNNKLIGARTFNLANNVSIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGM 240

Query: 241 APLAHVAMYKVCS-KGCQSSDILAGLDAAIDDGVDVLSISIGSGS--------------- 300
           APLAH+A+YKVCS KGC SSDILA LDAAIDDGVDVLS+S+G+ S               
Sbjct: 241 APLAHIAVYKVCSPKGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAA 300

Query: 301 ------------------------------VGASTIDRTIAALAKLGNDEVHVGESLFQP 360
                                         VGASTIDR I ALAKL + +V  GESLFQP
Sbjct: 301 IKKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQP 360

Query: 361 RDFTSTFLPLVYAGNSGTKRSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNAG 420
           RDF+S FLPLVYAG SG + SE+CV+GSL  L+V GK+VVCE GG IGR+ KGLVVKN G
Sbjct: 361 RDFSSKFLPLVYAGKSGIEGSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGG 420

Query: 421 GAAMILINQKPNGFTTSADAHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGGS 480
           GAAMIL+NQKP+GF+T A+AHVLP T +SYE GLKIK YI+SS NP ASISF+GT++G  
Sbjct: 421 GAAMILVNQKPDGFSTLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNR 480

Query: 481 NTDFSPAMASFSSRGPCLSSHGILKPDITGPGVNILAAWPFPI--DSNKNTKSTFNVMSG 540
            T FSPAMASFSSRGPC +S GILKPDITGPGVNILAAWPFP+  ++N NTKSTFNV+SG
Sbjct: 481 ATTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISG 540

Query: 541 TSMSCPHLSGIAALIKNVHPNWSPAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFATGS 600
           TSMSCPHLSGIAALIK+ HPNWSPAAIKSAIMTSADV+ PQGKPIVDQDLKPANFFA GS
Sbjct: 541 TSMSCPHLSGIAALIKSNHPNWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGS 600

Query: 601 GHVNPSKAADPGLVYDIQADDYVRYLCGLYTDVQVSMIVRRQITCSTVSKIREGDLNYPS 660
           GHVNPSKAA+PGLVYDIQ DDYV YLC LYTD QVS+IVRRQ+TCSTVS+IREGDLNYPS
Sbjct: 601 GHVNPSKAANPGLVYDIQPDDYVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPS 660

Query: 661 FSVTLGA-SQTFSRTVTNVGDANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVTF 704
           F+V+LGA SQ F+RTVTNVGDANSVY AIV+AP GVSVRVTP NLKFS LNEK+TYSVTF
Sbjct: 661 FAVSLGADSQAFNRTVTNVGDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTF 720

BLAST of HG10009218 vs. NCBI nr
Match: XP_008437177.1 (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo])

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 567/753 (75.30%), Postives = 625/753 (83.00%), Query Frame = 0

Query: 1   MVVLPLFCIFFLLNFHGNVAMKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLESWYT 60
           MV LP  CIFF LNFHG VAMKT +S TSNT  LQTYIVHV+QP LE   DT DL++WYT
Sbjct: 1   MVFLPFICIFFFLNFHGYVAMKTELSSTSNT-NLQTYIVHVKQPVLEILGDTIDLQNWYT 60

Query: 61  SFLPKTIE-TSNEQSRLLYSYRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTT 120
           SFLP+TIE +S+EQ RLLYSYRHVMSGFSARLT+EQVK+MEEKDGFISA PET LNLHTT
Sbjct: 61  SFLPETIEASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTT 120

Query: 121 HTPEYLGLNQQFGLWKDSNFGKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCEFGA 180
           HTPEYLGLN+ FGLWK+SNFGKGVIIGVLDTGI+P+HPSFND+GM  PPAKWKGRCEFGA
Sbjct: 121 HTPEYLGLNKHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGA 180

Query: 181 SICNNKLIGARTFNLANTVLMGETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGI 240
           SICNNKLIGARTFNLAN  ++G++P DENGHGTHTASTAAGTFV+GAEALGNARGKAVG+
Sbjct: 181 SICNNKLIGARTFNLANNFVIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGM 240

Query: 241 APLAHVAMYKVCS-KGCQSSDILAGLDAAIDDGVDVLSISIGSGS--------------- 300
           APLAH+AMYKVCS  GC SSDILA LDAAIDDGVDVLS+S+G+ S               
Sbjct: 241 APLAHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAMGAFAA 300

Query: 301 ------------------------------VGASTIDRTIAALAKLGNDEVHVGESLFQP 360
                                         VGASTIDR I ALAKLG+ EV+ GESLFQP
Sbjct: 301 IKKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQP 360

Query: 361 RDFTSTFLPLVYAGNSGTKRSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNAG 420
           R+F S FLPLVYAG SG + SE+C+QGSL  L+V GKVVVCE GG I RV KGLVVKN G
Sbjct: 361 RNFPSKFLPLVYAGKSGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGG 420

Query: 421 GAAMILINQKPNGFTTSADAHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGGS 480
           GAAMILINQKP GF+T A+AHVLP T +SYE GLKIKAYI+SS NP ASISFKGT++G  
Sbjct: 421 GAAMILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNR 480

Query: 481 NTDFSPAMASFSSRGPCLSSHGILKPDITGPGVNILAAWPFPI--DSNKNTKSTFNVMSG 540
            T FSPAMASFSSRGPC +S GILKPDITGPGVNILAAWPFP+  ++N NTKSTFNV+SG
Sbjct: 481 ATTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISG 540

Query: 541 TSMSCPHLSGIAALIKNVHPNWSPAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFATGS 600
           TSMSCPHLSGIAALIK+ HPNWSPAAIKSAIMTSADV+ PQGKPI+DQDLKPANFFA GS
Sbjct: 541 TSMSCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGS 600

Query: 601 GHVNPSKAADPGLVYDIQADDYVRYLCGLYTDVQVSMIVRRQITCSTVSKIREGDLNYPS 660
           GHVNPSKAA+PGLVYDIQ DDY+ YLC LYTD QVS+IVR+Q+TCSTV +IREGDLNYPS
Sbjct: 601 GHVNPSKAANPGLVYDIQPDDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPS 660

Query: 661 FSVTLGA-SQTFSRTVTNVGDANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVTF 704
           F+V+LGA SQTF+RTVTNVGDANSVY  IVEAP GVSV+VTPSNLKFS LNEK+TYSVTF
Sbjct: 661 FAVSLGAGSQTFNRTVTNVGDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTF 720

BLAST of HG10009218 vs. NCBI nr
Match: KAA0042822.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])

HSP 1 Score: 1060.4 bits (2741), Expect = 6.5e-306
Identity = 551/733 (75.17%), Postives = 608/733 (82.95%), Query Frame = 0

Query: 21  MKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLESWYTSFLPKTIE-TSNEQSRLLYS 80
           MKT +S TSNT  LQTYIVHV+QP LE   DT DL++WYTSFLP+TIE +S+EQ RLLYS
Sbjct: 1   MKTELSSTSNT-NLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYS 60

Query: 81  YRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNF 140
           YRHVMSGFSARLT+EQVK+MEEKDGFISA PET LNLHTTHTPEYLGLN  FGLWK+SNF
Sbjct: 61  YRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNF 120

Query: 141 GKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNLANTVL 200
           GKGVIIGVLDTGI+P+HPSFND+GM  PPAKWKGRCEFGASICNNKLIGARTFNLAN  +
Sbjct: 121 GKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFV 180

Query: 201 MGETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGIAPLAHVAMYKVCS-KGCQSS 260
           +G++P DENGHGTHTASTAAGTFV+GAEALGNARGKAVG+APLAH+AMYKVCS  GC SS
Sbjct: 181 IGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSS 240

Query: 261 DILAGLDAAIDDGVDVLSISIGSGS----------------------------------- 320
           DILA LDAAIDDGVDVLS+S+G+ S                                   
Sbjct: 241 DILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNT 300

Query: 321 ----------VGASTIDRTIAALAKLGNDEVHVGESLFQPRDFTSTFLPLVYAGNSGTKR 380
                     VGASTIDR I ALAKLG+ EV+ GESLFQPR+F S FLPLVYAG SG + 
Sbjct: 301 LANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEG 360

Query: 381 SEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNAGGAAMILINQKPNGFTTSADA 440
           SE+C+QGSL  L+V GKVVVCE GG I RV KGLVVKN GGAAMILINQKP GF+T A+A
Sbjct: 361 SEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEA 420

Query: 441 HVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGGSNTDFSPAMASFSSRGPCLSS 500
           HVLP T +SYE GLKIKAYI+SS NP ASISFKGT++G   T FSPAMASFSSRGPC +S
Sbjct: 421 HVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQAS 480

Query: 501 HGILKPDITGPGVNILAAWPFPI--DSNKNTKSTFNVMSGTSMSCPHLSGIAALIKNVHP 560
            GILKPDITGPGVNILAAWPFP+  ++N NTKSTFNV+SGTSMSCPHLSGIAALIK+ HP
Sbjct: 481 PGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHP 540

Query: 561 NWSPAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFATGSGHVNPSKAADPGLVYDIQAD 620
           NWSPAAIKSAIMTSADV+ PQGKPI+DQDLKPANFFA GSGHVNPSKAA+PGLVYDIQ D
Sbjct: 541 NWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPD 600

Query: 621 DYVRYLCGLYTDVQVSMIVRRQITCSTVSKIREGDLNYPSFSVTLGA-SQTFSRTVTNVG 680
           DY+ YLC LYTD QVS+IVR+Q+TCSTV +IREGDLNYPSF+V+L A SQTF+RTVTNVG
Sbjct: 601 DYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVG 660

Query: 681 DANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVTFSVDALVKVTSEFSEGYLKWV 704
           DANSVY  IVEAP GVSV+VTPSNLKFS LNEK+TYSVTFS    V+ TSEFSEGYL WV
Sbjct: 661 DANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWV 720

BLAST of HG10009218 vs. NCBI nr
Match: KAA0042821.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])

HSP 1 Score: 1003.4 bits (2593), Expect = 9.5e-289
Identity = 519/756 (68.65%), Postives = 600/756 (79.37%), Query Frame = 0

Query: 1   MVVLPLFCIFFLLNFHGNVAMKTNVSDTSNTIKLQTYIVHVQQPEL----ESSDDTFDLE 60
           MVVL    IFFLLN   +VA K  +  T    KLQTYIVHV+QP++    ESS+D   +E
Sbjct: 1   MVVLRFLSIFFLLNIESHVAAKVELPTTPLQSKLQTYIVHVEQPKIRLVGESSND--HIE 60

Query: 61  SWYTSFLPKTIETSNEQSRLLYSYRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNL 120
           SWY SF+PK+ ET+ EQ +LLYSYR+VMSGFSARLT EQVK+ME+KDGFISA PETI++L
Sbjct: 61  SWYMSFIPKSTETTVEQPQLLYSYRNVMSGFSARLTIEQVKAMEKKDGFISAMPETIMSL 120

Query: 121 HTTHTPEYLGLNQQFGLWKDSNFGKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCE 180
           HTTHTPEYLGLNQQFG+WK+SNFGKGVI+GVLDTGI+P+HPSFND+GM  PPAKWKG+CE
Sbjct: 121 HTTHTPEYLGLNQQFGIWKNSNFGKGVIVGVLDTGIHPNHPSFNDEGMSPPPAKWKGKCE 180

Query: 181 FGASICNNKLIGARTFNLANTVLMGETPIDENGHGTHTASTAAGTFVEGAEALGNARGKA 240
           F +S+CNNKLIGARTFNL N  LM E+P DE GHGTHTASTAAG FVE AEALGNA+GKA
Sbjct: 181 FNSSLCNNKLIGARTFNLGNNFLMEESPNDEKGHGTHTASTAAGAFVEDAEALGNAKGKA 240

Query: 241 VGIAPLAHVAMYKVCS-KGCQSSDILAGLDAAIDDGVDVLSISIGSGS------------ 300
            GIAPLAH+A+YKVCS K C SSD+ AG+DAAIDDGVDVLSIS+GS S            
Sbjct: 241 AGIAPLAHLAIYKVCSGKRCPSSDVFAGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVAT 300

Query: 301 ---------------------------------VGASTIDRTIAALAKLGNDEVHVGESL 360
                                            VGASTI+R I A+AKLGN E + GESL
Sbjct: 301 FGAIQKGIFVSSSAGNSGPLNSTLSNDAPWILTVGASTINRRIVAVAKLGNGEQYEGESL 360

Query: 361 FQPRDFTSTFLPLVYAGNSGTKRSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVK 420
           +QP DF S FLPLVYAGN   K   FC +GSL N+DVKGKVVVCE  G +GRV KGLVVK
Sbjct: 361 YQPNDFPSKFLPLVYAGNRENKTYAFCGEGSLENMDVKGKVVVCEGKGGVGRVAKGLVVK 420

Query: 421 NAGGAAMILINQKPNGFTTSADAHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTII 480
           NAGGAAMILINQ+ +GF+T ++AHVLPAT VSY+AG+ IK+YI+SSQNPTASISFKGT+I
Sbjct: 421 NAGGAAMILINQEEDGFSTLSEAHVLPATHVSYKAGVLIKSYINSSQNPTASISFKGTVI 480

Query: 481 GGSNTDFSPAMASFSSRGPCLSSHGILKPDITGPGVNILAAWPFPIDSNKNTKSTFNVMS 540
           G  +   +P+MASFSSRGPCL S GILKPDITGPGVNILAAWPFP+D++ NTKSTFNV+S
Sbjct: 481 GDGDDFSAPSMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDNDTNTKSTFNVIS 540

Query: 541 GTSMSCPHLSGIAALIKNVHPNWSPAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFATG 600
           GTSMSCPHLSGIAALIK+VHPNWSPAAIKSAIMT+A++KTPQG+PIVDQDL+PANFFA G
Sbjct: 541 GTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTANIKTPQGEPIVDQDLQPANFFAMG 600

Query: 601 SGHVNPSKAADPGLVYDIQADDYVRYLCGLYTDVQVSMIVRRQITCSTVSKIREGDLNYP 660
           +GHVNPSKAADPGLVYDIQ DDY+ YLCGLY D +VS+IV R + C  V  IREGDLNYP
Sbjct: 601 AGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDEEVSIIVHRTVICGLVPSIREGDLNYP 660

Query: 661 SFSVTLGASQTFSRTVTNVGDANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVTF 705
           SFSV LG  QTF RTVTNVG+ANSVY+AIVEAP+GVS+ V P  L FS +N+ +T++VTF
Sbjct: 661 SFSVALGGLQTFKRTVTNVGEANSVYTAIVEAPLGVSMTVMPRKLFFSRVNQTMTFTVTF 720

BLAST of HG10009218 vs. ExPASy Swiss-Prot
Match: A9QY38 (Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 3.0e-237
Identity = 434/749 (57.94%), Postives = 536/749 (71.56%), Query Frame = 0

Query: 11  FLLNFHGNVA----MKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLESWYTSFLPKT 70
           FLL FH + A    + T  ++++ T   + YI+HV  PE +   ++ DLESWY SFLP T
Sbjct: 11  FLLTFHVHNAQGSELPTTTTESTETSSSKIYIIHVTGPEGKMLTESEDLESWYHSFLPPT 70

Query: 71  IETSNEQSRLLYSYRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTTHTPEYLG 130
           + +S EQ R++YSY++V+ GF+A LT+E++ ++E+K+GFISA P+ +L+  TTHTP++LG
Sbjct: 71  LMSSEEQPRVIYSYKNVLRGFAASLTQEELSAVEKKNGFISAHPQRVLHRQTTHTPKFLG 130

Query: 131 LNQQFGLWKDSNFGKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCEFGASICNNKL 190
           L Q  G+WK+SNFGKGVIIGVLD+GI P HPSF+D G+P PP KWKGRC+   + CNNKL
Sbjct: 131 LQQDTGVWKESNFGKGVIIGVLDSGITPGHPSFSDVGIPPPPPKWKGRCDLNVTACNNKL 190

Query: 191 IGARTFNLANTVLMG---ETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGIAPLA 250
           IGAR FNLA   + G   E PIDE+GHGTHTASTAAG FV  AE LGNA+G A G+AP A
Sbjct: 191 IGARAFNLAAEAMNGKKAEAPIDEDGHGTHTASTAAGAFVNYAEVLGNAKGTAAGMAPHA 250

Query: 251 HVAMYKVC-SKGCQSSDILAGLDAAIDDGVDVLSISIG---------------------- 310
           H+A+YKVC  + C  SDILA LDAA++DGVDV+SIS+G                      
Sbjct: 251 HLAIYKVCFGEDCPESDILAALDAAVEDGVDVISISLGLSEPPPFFNDSTAIGAFAAMQK 310

Query: 311 ----------SG--------------SVGASTIDRTIAALAKLGNDEVHVGESLFQPRDF 370
                     SG              +VGASTIDR I A AKLGN +   GES+FQP  F
Sbjct: 311 GIFVSCAAGNSGPFNSSIVNAAPWILTVGASTIDRRIVATAKLGNGQEFDGESVFQPSSF 370

Query: 371 TSTFLPLVYAGNSGTKRSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNAGGAA 430
           T T LPL YAG +G + S FC  GSL +   +GKVV+CE GG I R+ KG  VK AGGAA
Sbjct: 371 TPTLLPLAYAGKNGKEESAFCANGSLDDSAFRGKVVLCERGGGIARIAKGEEVKRAGGAA 430

Query: 431 MILINQKPNGFTTSADAHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGGSNTD 490
           MIL+N + N F+ SAD H LPAT VSY AG++IKAYI+S+  PTA+I FKGT+IG S   
Sbjct: 431 MILMNDETNAFSLSADVHALPATHVSYAAGIEIKAYINSTATPTATILFKGTVIGNS--- 490

Query: 491 FSPAMASFSSRGPCLSSHGILKPDITGPGVNILAAWPFPIDSNKNTKSTFNVMSGTSMSC 550
            +PA+ASFSSRGP L S GILKPDI GPGVNILAAWPFP+ ++ ++K TFN+ SGTSMSC
Sbjct: 491 LAPAVASFSSRGPNLPSPGILKPDIIGPGVNILAAWPFPLSNSTDSKLTFNIESGTSMSC 550

Query: 551 PHLSGIAALIKNVHPNWSPAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFATGSGHVNP 610
           PHLSGIAAL+K+ HP+WSPAAIKSAIMTSAD      K IVD+ L+P + FATGSGHVNP
Sbjct: 551 PHLSGIAALLKSSHPHWSPAAIKSAIMTSADTINLGNKLIVDETLQPTDLFATGSGHVNP 610

Query: 611 SKAADPGLVYDIQADDYVRYLCGL-YTDVQVSMIVRRQITCSTVSKIREGDLNYPSFSVT 670
           S+A DPGLVYDIQ DDY+ YLCGL Y++ +V +I  R+I CS  + I EG+LNYPSFSV 
Sbjct: 611 SRANDPGLVYDIQPDDYIPYLCGLGYSETEVGIIAHRKIKCS--ASIPEGELNYPSFSVE 670

Query: 671 LGASQTFSRTVTNVGDANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVTFSVDAL 705
           LG+S+TF+RTVTNVG+A+S Y  IV AP GV V+V P  L FS +N+K TYSVTFS   L
Sbjct: 671 LGSSKTFTRTVTNVGEAHSSYDLIVAAPQGVDVKVQPYKLNFSEVNQKETYSVTFSRTGL 730

BLAST of HG10009218 vs. ExPASy Swiss-Prot
Match: A0A0M3R8G2 (Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1)

HSP 1 Score: 747.7 bits (1929), Expect = 1.2e-214
Identity = 403/754 (53.45%), Postives = 512/754 (67.90%), Query Frame = 0

Query: 1   MVVLPLFCIFFLLNFHGNVAMKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLESWYT 60
           M  + +F I FLL+FH   A ++          LQTYIVHV +P+ +   ++ DLES+Y 
Sbjct: 1   MTCICIFSIAFLLSFHLTTAQRST--------GLQTYIVHVDKPDAQVLANSADLESYYN 60

Query: 61  SFLPKTIETSNEQSRLLYSYRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTTH 120
           SFLP T+  S   SR+++SY HV +GF+A+L+ E+VK ME+K GF+SA+ E +L LHTTH
Sbjct: 61  SFLPATVSGSEVPSRIIHSYHHVATGFAAKLSGEEVKEMEKKAGFVSAKLEKVLTLHTTH 120

Query: 121 TPEYLGLNQQFGLWKDSNFGKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCEF-GA 180
           TP +LGL Q  G W++SN+GKGVIIG+LDTGI P HPSF+D  MP PPAKWKG+CEF G 
Sbjct: 121 TPNFLGLYQNMGFWQESNYGKGVIIGLLDTGITPGHPSFSDVNMPSPPAKWKGKCEFTGN 180

Query: 181 SICNNKLIGARTFNLANTVLMGETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGI 240
           + CN K+IGAR F   +    G  P DE GHGTHTASTAAG FV  A   GNA G AVG+
Sbjct: 181 ATCNKKIIGARNFISGS----GVPPTDEEGHGTHTASTAAGNFVNDANVFGNANGTAVGM 240

Query: 241 APLAHVAMYKVCSK-GCQSSDILAGLDAAIDDGVDVLSISIGSGS--------------- 300
           APLAH+AMYKVCS+ GC  +DILA LDAAIDDGVDVLS+S+G  S               
Sbjct: 241 APLAHIAMYKVCSEDGCSDADILAALDAAIDDGVDVLSLSLGGYSDPFYYDNIAIGAFAA 300

Query: 301 ------------------------------VGASTIDRTIAALAKLGNDEVHVGESLFQP 360
                                         VGAST DR I A A LGN + + GES FQP
Sbjct: 301 IRKGIFVSASAGNDGPLNSTLSNEAPWILTVGASTHDRKIVATAVLGNGQQYDGESAFQP 360

Query: 361 RDFTSTFLPLVYAGNSGTKRSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNAG 420
            DF  T LPLVY G S  + + FC  GSL   DVKGKVVVC+ GG++ R++K   VK+AG
Sbjct: 361 ADFPHTLLPLVYPGTS-DEEAAFCSSGSLDKFDVKGKVVVCDRGGDVARLEKSQTVKDAG 420

Query: 421 GAAMILINQKPNGFTTSADAHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGGS 480
           GAAMIL N + +G  T ADAHVLPAT V Y AG  IK+YI+S+  PTA I FKGTIIG  
Sbjct: 421 GAAMILANLEIDGEGTFADAHVLPATHVGYAAGEMIKSYINSTSTPTAGILFKGTIIGFK 480

Query: 481 NTDFSPAMASFSSRGPCLSSHGILKPDITGPGVNILAAWPFPIDSNKNTKSTFNVMSGTS 540
           +   SP+++SFSSRGP L+S GI+KPDI GPGVNILAAWP  +++   T  TFN++SGTS
Sbjct: 481 S---SPSVSSFSSRGPNLASPGIVKPDIIGPGVNILAAWPVSVENKTGTDLTFNIISGTS 540

Query: 541 MSCPHLSGIAALIKNVHPNWSPAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFATGSGH 600
           MSCPHLSGI AL+K+ HP+WSPAAIKSAIMTSAD    +G+PI+D+   PA+ FATG+GH
Sbjct: 541 MSCPHLSGIVALLKSAHPDWSPAAIKSAIMTSADQSNLEGQPILDERNLPADIFATGAGH 600

Query: 601 VNPSKAADPGLVYDIQADDYVRYLCGL-YTDVQVSMIVRRQITCSTVSKIREGDLNYPSF 660
           VNPSKA+DPGL+YDIQ +DY++YLCGL Y +  + +IV+  + C   S I E +LNYPSF
Sbjct: 601 VNPSKASDPGLIYDIQLEDYIQYLCGLGYREKDIGLIVQETVKCE--SSISEAELNYPSF 660

Query: 661 SVTLG-ASQTFSRTVTNVGDANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVTFS 706
           S+ LG  +Q ++RTVTNVGDA+S Y+  +    GV + V P+ L F+ + ++ TY+V+F+
Sbjct: 661 SIILGPKTQNYTRTVTNVGDASSTYTVNIAKTQGVDIVVEPATLVFTKMYQQATYTVSFT 720

BLAST of HG10009218 vs. ExPASy Swiss-Prot
Match: A9QY39 (Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1)

HSP 1 Score: 727.2 bits (1876), Expect = 1.7e-208
Identity = 393/728 (53.98%), Postives = 504/728 (69.23%), Query Frame = 0

Query: 27  DTSNTIKLQTYIVHVQQPELESSDDTFDLESWYTSFLPKTIETSNEQSRLLYSYRHVMSG 86
           D SN   L TYIVHV++P++  SD   DL ++Y S LP++ +T+N+  R++++YR+V++G
Sbjct: 38  DQSN---LSTYIVHVRKPQVIQSD---DLHTFYYSLLPESTKTTNQ--RIVFTYRNVVNG 97

Query: 87  FSARLTEEQVKSMEEKDGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNFGKGVIIG 146
           F+ +LT E+ K++++ +  +SARPE IL+LHTTHTP +LGL Q  GLWK SN GKGVIIG
Sbjct: 98  FAVKLTPEEAKALQQNEEVVSARPEKILSLHTTHTPSFLGLQQGLGLWKGSNSGKGVIIG 157

Query: 147 VLDTGINPDHPSFNDDGMPQPPAKWKGRCEF-GASICNNKLIGARTFNLANTVLMGETPI 206
           +LDTGI+P HPSF+D+GMP PPAKW G CEF G   CNNK+IGAR F     + +   P 
Sbjct: 158 ILDTGISPFHPSFSDEGMPSPPAKWNGICEFTGKRTCNNKIIGARNFVKTKNLTL---PF 217

Query: 207 DENGHGTHTASTAAGTFVEGAEALGNARGKAVGIAPLAHVAMYKVCS-KGCQSSDILAGL 266
           D+ GHGTHTASTAAG  V+GA   GNA G AVG+AP AH+AMYKVC   GC  S ILAG+
Sbjct: 218 DDVGHGTHTASTAAGRPVQGANVYGNANGTAVGMAPDAHIAMYKVCGLVGCSESAILAGM 277

Query: 267 DAAIDDGVDVLSISIGSGS----------------------------------------- 326
           D A+DDGVDVLS+S+G  S                                         
Sbjct: 278 DTAVDDGVDVLSLSLGGPSGPFFEDPIALGAFGAIQKGIFVSCSAANSGPAYSSLSNEAP 337

Query: 327 ----VGASTIDRTIAALAKLGNDEVHVGESLFQPRDFTSTFLPLVYAGNSGTKR-SEFCV 386
               VGAS+IDRTI A AKLGN + +VG+S+FQP+DF  + LPLVYAG +G    S FC 
Sbjct: 338 WILTVGASSIDRTIMATAKLGNGKEYVGQSVFQPKDFAPSLLPLVYAGANGNNNFSVFCA 397

Query: 387 QGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNAGGAAMILINQKPNGFTTSADAHVLPA 446
             SL   DV+GKVV+CE GG + RV KG  VK+AGGAAMIL+N     F   AD HVLPA
Sbjct: 398 PESLNRSDVEGKVVLCEDGGFVPRVFKGKAVKDAGGAAMILMNSVLEDFNPIADVHVLPA 457

Query: 447 TRVSYEAGLKIKAYISSSQNPTASISFKGTIIGGSNTDFSPAMASFSSRGPCLSSHGILK 506
             +SYEAGL +K YI+S+  PTA+I F+GT+IG      +P + SFSSRGP  +S GILK
Sbjct: 458 VHISYEAGLALKEYINSTSTPTATILFEGTVIGNL---LAPQVTSFSSRGPSKASPGILK 517

Query: 507 PDITGPGVNILAAWPFPIDSNKNTKSTFNVMSGTSMSCPHLSGIAALIKNVHPNWSPAAI 566
           PDI GPG+NILAAWP  +D+  +T   FN++SGTSMSCPHLSGIAAL+KN HP+WSPAAI
Sbjct: 518 PDIIGPGLNILAAWPVSLDN--STTPPFNIISGTSMSCPHLSGIAALLKNSHPDWSPAAI 577

Query: 567 KSAIMTSADVKTPQGKPIVDQDLKPANFFATGSGHVNPSKAADPGLVYDIQADDYVRYLC 626
           KSAIMT+A      G PI+DQ L PA+ FATG+GHVNP KA DPGLVYDI+ +DY+ YLC
Sbjct: 578 KSAIMTTASQVNLGGTPILDQRLVPADVFATGAGHVNPVKANDPGLVYDIEPNDYIPYLC 637

Query: 627 GL-YTDVQVSMIVRRQITCSTVSKIREGDLNYPSFSVTLG-ASQTFSRTVTNVGDANSVY 686
           GL YTD +V +I+++++ CS V+ I E +LNYPSFS+ LG  +Q ++RTV NVG ANS Y
Sbjct: 638 GLNYTDREVGVILQQRVRCSEVNHIAEAELNYPSFSILLGNTTQLYTRTVANVGPANSTY 697

Query: 687 SAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVTFSVDALVKVTSEFSEGYLKWVSDKHLV 705
           +A +  PVGV + ++P+ L F+ + +K+TYSV+F   +  +    F++G LKWVS K+ V
Sbjct: 698 TAEIGVPVGVGMSLSPAQLTFTEVGQKLTYSVSFIPFSEDRDNHTFAQGSLKWVSGKYSV 749

BLAST of HG10009218 vs. ExPASy Swiss-Prot
Match: A9QY40 (Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1)

HSP 1 Score: 717.6 bits (1851), Expect = 1.4e-205
Identity = 394/726 (54.27%), Postives = 498/726 (68.60%), Query Frame = 0

Query: 34  LQTYIVHVQQPELESS-DDTFDLESWYTSFLPKTIETSNEQSRLLYSYRHVMSGFSARLT 93
           L TYIVHV++ E+E     T +L +W+ SFLP   ETSN + R+++SYR+V SGF+ RLT
Sbjct: 38  LTTYIVHVKKLEIEGPLQSTEELHTWHHSFLP---ETSN-KDRMVFSYRNVASGFAVRLT 97

Query: 94  EEQVKSMEEKDGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNFGKGVIIGVLDTGI 153
            E+  +++EK+  +S RPE  L+LHTTHTP +LGL Q  GLW DSN GKGVIIGV+DTGI
Sbjct: 98  PEEANALQEKEEVMSIRPERTLSLHTTHTPSFLGLRQGQGLWNDSNLGKGVIIGVIDTGI 157

Query: 154 NPDHPSFNDDGMPQPPAKWKGRCEF-GASICNNKLIGARTFNLANTVLMGETPIDENGHG 213
            P H SFND+GMP PPAKWKG CEF G S+CNNKLIGAR  NL  + +  E P ++  HG
Sbjct: 158 YPFHLSFNDEGMPPPPAKWKGHCEFTGGSVCNNKLIGAR--NLVKSAIQ-EPPYEDFFHG 217

Query: 214 THTASTAAGTFVEGAEALGNARGKAVGIAPLAHVAMYKVCSK----GCQSSDILAGLDAA 273
           THTA+ AAG FVEGA   GNARG A G+AP AH+A+YKVCS      C  S ILA +D A
Sbjct: 218 THTAAEAAGRFVEGASVFGNARGTAAGMAPDAHLAIYKVCSSKVKDECPESAILAAMDIA 277

Query: 274 IDDGVDVLSISIGSGS-------------------------------------------- 333
           I+DGVDVLS+S+G GS                                            
Sbjct: 278 IEDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQKGIFVSCSAANSGPHYSSLSNEAPWIL 337

Query: 334 -VGASTIDRTIAALAKLGNDEVHVGESLFQPRDFTSTFLPLVYAGNSGTKRSEFCVQGSL 393
            VGASTIDR I+A AKLGN   + GE+LFQP+DF+S  LPLVYA       S  C  GSL
Sbjct: 338 TVGASTIDRKISASAKLGNGAEYEGETLFQPKDFSSQLLPLVYAAAEKNNSSALCAPGSL 397

Query: 394 ANLDVKGKVVVCETGGNIGRVKKGLVVKNAGGAAMILINQKPNGFTTSADAHVLPATRVS 453
            N++VKGKVVVC+ GG I  + KG  V +AGG+AMIL N +  GFTT A+AHVLPA  VS
Sbjct: 398 RNINVKGKVVVCDLGGGIPFIAKGQEVLDAGGSAMILANIENFGFTTLANAHVLPAVHVS 457

Query: 454 YEAGLKIKAYISSSQNPTASISFKGTIIGGSNTDFSPAMASFSSRGPCLSSHGILKPDIT 513
           Y A L IKAYI+S+  PTA++ F+GTIIG S    +P++A+FSSRGP   S GILKPDI 
Sbjct: 458 YAASLAIKAYINSTYTPTATVLFQGTIIGDS---LAPSVAAFSSRGPSQQSPGILKPDII 517

Query: 514 GPGVNILAAWPFPIDSNKNTKSTFNVMSGTSMSCPHLSGIAALIKNVHPNWSPAAIKSAI 573
           GPGVNILAAW   +D   N    F+++SGTSMSCPHLSGIAAL+K+ HP+WSPAAIKSAI
Sbjct: 518 GPGVNILAAWAVSVD---NKIPAFDIISGTSMSCPHLSGIAALLKSAHPDWSPAAIKSAI 577

Query: 574 MTSADVKTPQGKPIVDQDLKPANFFATGSGHVNPSKAADPGLVYDIQADDYVRYLCGL-Y 633
           MT+A+    +G PI+DQ L+PA+ FATG+GHVNP +A DPGLVYDIQ +DYV YLCGL Y
Sbjct: 578 MTTANTLNLRGLPILDQRLQPADIFATGAGHVNPVRANDPGLVYDIQPEDYVPYLCGLGY 637

Query: 634 TDVQVSMIVRRQITCSTVSKIREGDLNYPSFSVTLGA-SQTFSRTVTNVGDANSVYSAIV 693
           +D +V++IV+R + C  V  I + +LNYPSFS+ LG+ SQ ++RT+TNVG ANS Y+  +
Sbjct: 638 SDREVTIIVQRSVRCFNVKSIAQAELNYPSFSILLGSDSQFYTRTLTNVGPANSTYTVKI 697

Query: 694 EAPVGVSVRVTPSNLKFSSLNEKITYSVTFSVDALV-KVTSEFSEGYLKWVSDKHLVRSP 706
           + P+ + + V+PS + F+ +N+K+ Y V F       +    F++G + WVSDKH+VR+P
Sbjct: 698 DVPLAMGISVSPSQITFTQVNQKVAYFVDFIPQIKENRGNHTFAQGAITWVSDKHVVRTP 750

BLAST of HG10009218 vs. ExPASy Swiss-Prot
Match: G7KEU7 (Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1)

HSP 1 Score: 712.2 bits (1837), Expect = 5.7e-204
Identity = 397/755 (52.58%), Postives = 498/755 (65.96%), Query Frame = 0

Query: 6   LFCIFFLLNFHGNVAMKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLESWYTSFLPK 65
           L  + F+L     +A + N+    + I L TYIVHV++ E  +S  + DL SWY SFLP+
Sbjct: 9   LVSLIFILCSISMLAAEENLE--HDQINLMTYIVHVKKSENVASHQSEDLHSWYHSFLPQ 68

Query: 66  TIETSNEQSRLLYSYRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTTHTPEYL 125
           T      + R+++SYR V SGF+ +LT E+ KS++EK   +SARPE  L LHTTHTP +L
Sbjct: 69  TFP---HKERMVFSYRKVASGFAVKLTPEEAKSLQEKGEIVSARPERTLELHTTHTPTFL 128

Query: 126 GLNQQFGLWKDSNFGKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCEF-GASICNN 185
           GL Q  GLW D N GKGVIIG++DTGI P HPSFND+GMP PPAKWKG CEF G  +CNN
Sbjct: 129 GLKQGQGLWSDDNLGKGVIIGIIDTGIFPLHPSFNDEGMPPPPAKWKGHCEFTGGQVCNN 188

Query: 186 KLIGARTFNLANTVLMGETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGIAPLAH 245
           KLIGAR  NL  + +  E P +   HGTHTA+ AAG F+E A   GNA+G A G+AP AH
Sbjct: 189 KLIGAR--NLVKSAIQ-EPPFENFFHGTHTAAEAAGRFIEDASVFGNAKGVAAGMAPNAH 248

Query: 246 VAMYKVCSK--GCQSSDILAGLDAAIDDGVDVLSISIGSGS------------------- 305
           +A+YKVC+   GC  S ILA +D AI+DGVDVLS+S+G GS                   
Sbjct: 249 LAIYKVCNDKIGCTESAILAAMDIAIEDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQNG 308

Query: 306 --------------------------VGASTIDRTIAALAKLGNDEVHVGESLFQPRDFT 365
                                     VGASTIDR I A AKLGN E + GE+LFQP+DF+
Sbjct: 309 VFVSCSAANSGPGYSTLSNEAPWILTVGASTIDRKIVASAKLGNGEEYEGETLFQPKDFS 368

Query: 366 STFLPLVYAGNSG----TKRSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNAG 425
              LPLVY G+ G    T+    C+ GSL N+D+ GKVV+C+  GN+  + KG  V N+G
Sbjct: 369 QQLLPLVYPGSFGYGNQTQNQSLCLPGSLKNIDLSGKVVLCDV-GNVSSIVKGQEVLNSG 428

Query: 426 GAAMILINQKPNGFTTSADAHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGGS 485
           G AMIL N +  GF+T A AHVLPA  VSY AGL IK+YI S+ NPTA++ FKGTIIG S
Sbjct: 429 GIAMILANSEALGFSTFAIAHVLPAVEVSYAAGLTIKSYIKSTYNPTATLIFKGTIIGDS 488

Query: 486 NTDFSPAMASFSSRGPCLSSHGILKPDITGPGVNILAAWPFPIDSNKNTKSTFNVMSGTS 545
               +P++  FSSRGP   S GILKPDI GPGVNILAAW   +D   N    F+++SGTS
Sbjct: 489 ---LAPSVVYFSSRGPSQESPGILKPDIIGPGVNILAAWAVSVD---NKIPAFDIVSGTS 548

Query: 546 MSCPHLSGIAALIKNVHPNWSPAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFATGSGH 605
           MSCPHLSGIAALIK+ HP+WSPAAIKSAIMT+A+     G PI+DQ L PA+ FATG+GH
Sbjct: 549 MSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTANTLNLGGIPILDQRLFPADIFATGAGH 608

Query: 606 VNPSKAADPGLVYDIQADDYVRYLCGL-YTDVQVSMIVRRQITCSTVSKIREGDLNYPSF 665
           VNP KA DPGLVYDI+ +DYV YLCGL Y+D ++ +IV+ ++ CS V  I E  LNYPSF
Sbjct: 609 VNPVKANDPGLVYDIEPEDYVPYLCGLGYSDKEIEVIVQWKVKCSNVKSIPEAQLNYPSF 668

Query: 666 SVTLGA-SQTFSRTVTNVGDANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVTFS 706
           S+ LG+ SQ ++RT+TNVG ANS Y   +E P+ + + V PS + F+ +NEK+++SV F 
Sbjct: 669 SILLGSDSQYYTRTLTNVGFANSTYKVELEVPLALGMSVNPSEITFTEVNEKVSFSVEFI 728

BLAST of HG10009218 vs. ExPASy TrEMBL
Match: A0A1S3AT16 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482683 PE=3 SV=1)

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 567/753 (75.30%), Postives = 625/753 (83.00%), Query Frame = 0

Query: 1   MVVLPLFCIFFLLNFHGNVAMKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLESWYT 60
           MV LP  CIFF LNFHG VAMKT +S TSNT  LQTYIVHV+QP LE   DT DL++WYT
Sbjct: 1   MVFLPFICIFFFLNFHGYVAMKTELSSTSNT-NLQTYIVHVKQPVLEILGDTIDLQNWYT 60

Query: 61  SFLPKTIE-TSNEQSRLLYSYRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTT 120
           SFLP+TIE +S+EQ RLLYSYRHVMSGFSARLT+EQVK+MEEKDGFISA PET LNLHTT
Sbjct: 61  SFLPETIEASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTT 120

Query: 121 HTPEYLGLNQQFGLWKDSNFGKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCEFGA 180
           HTPEYLGLN+ FGLWK+SNFGKGVIIGVLDTGI+P+HPSFND+GM  PPAKWKGRCEFGA
Sbjct: 121 HTPEYLGLNKHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGA 180

Query: 181 SICNNKLIGARTFNLANTVLMGETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGI 240
           SICNNKLIGARTFNLAN  ++G++P DENGHGTHTASTAAGTFV+GAEALGNARGKAVG+
Sbjct: 181 SICNNKLIGARTFNLANNFVIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGM 240

Query: 241 APLAHVAMYKVCS-KGCQSSDILAGLDAAIDDGVDVLSISIGSGS--------------- 300
           APLAH+AMYKVCS  GC SSDILA LDAAIDDGVDVLS+S+G+ S               
Sbjct: 241 APLAHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAMGAFAA 300

Query: 301 ------------------------------VGASTIDRTIAALAKLGNDEVHVGESLFQP 360
                                         VGASTIDR I ALAKLG+ EV+ GESLFQP
Sbjct: 301 IKKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQP 360

Query: 361 RDFTSTFLPLVYAGNSGTKRSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNAG 420
           R+F S FLPLVYAG SG + SE+C+QGSL  L+V GKVVVCE GG I RV KGLVVKN G
Sbjct: 361 RNFPSKFLPLVYAGKSGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGG 420

Query: 421 GAAMILINQKPNGFTTSADAHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGGS 480
           GAAMILINQKP GF+T A+AHVLP T +SYE GLKIKAYI+SS NP ASISFKGT++G  
Sbjct: 421 GAAMILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNR 480

Query: 481 NTDFSPAMASFSSRGPCLSSHGILKPDITGPGVNILAAWPFPI--DSNKNTKSTFNVMSG 540
            T FSPAMASFSSRGPC +S GILKPDITGPGVNILAAWPFP+  ++N NTKSTFNV+SG
Sbjct: 481 ATTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISG 540

Query: 541 TSMSCPHLSGIAALIKNVHPNWSPAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFATGS 600
           TSMSCPHLSGIAALIK+ HPNWSPAAIKSAIMTSADV+ PQGKPI+DQDLKPANFFA GS
Sbjct: 541 TSMSCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGS 600

Query: 601 GHVNPSKAADPGLVYDIQADDYVRYLCGLYTDVQVSMIVRRQITCSTVSKIREGDLNYPS 660
           GHVNPSKAA+PGLVYDIQ DDY+ YLC LYTD QVS+IVR+Q+TCSTV +IREGDLNYPS
Sbjct: 601 GHVNPSKAANPGLVYDIQPDDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPS 660

Query: 661 FSVTLGA-SQTFSRTVTNVGDANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVTF 704
           F+V+LGA SQTF+RTVTNVGDANSVY  IVEAP GVSV+VTPSNLKFS LNEK+TYSVTF
Sbjct: 661 FAVSLGAGSQTFNRTVTNVGDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTF 720

BLAST of HG10009218 vs. ExPASy TrEMBL
Match: A0A0A0KMQ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157280 PE=3 SV=1)

HSP 1 Score: 1067.4 bits (2759), Expect = 2.6e-308
Identity = 552/733 (75.31%), Postives = 613/733 (83.63%), Query Frame = 0

Query: 21  MKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLESWYTSFLPKTIE-TSNEQSRLLYS 80
           MKT +   SNT  LQTYIVHV+QPE+E   DT DL++WYTSFLP+TIE +SNEQSRLLYS
Sbjct: 1   MKTELWSASNT-NLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYS 60

Query: 81  YRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNF 140
           YRHV+SGFSARLT+EQVK+MEEKDGFISA PET LNLHTTHTPEYLGLNQ FGLWK+SNF
Sbjct: 61  YRHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNF 120

Query: 141 GKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNLANTVL 200
           GKGVIIGVLDTGI+P+HPSFND+GMP PPAKWKGRCEFGASICNNKLIGARTFNLAN V 
Sbjct: 121 GKGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVS 180

Query: 201 MGETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGIAPLAHVAMYKVCS-KGCQSS 260
           +G++P DENGHGTHTASTAAGTFV+GAEALGNARGKAVG+APLAH+A+YKVCS KGC SS
Sbjct: 181 IGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSS 240

Query: 261 DILAGLDAAIDDGVDVLSISIGSGS----------------------------------- 320
           DILA LDAAIDDGVDVLS+S+G+ S                                   
Sbjct: 241 DILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNT 300

Query: 321 ----------VGASTIDRTIAALAKLGNDEVHVGESLFQPRDFTSTFLPLVYAGNSGTKR 380
                     VGASTIDR I ALAKL + +V  GESLFQPRDF+S FLPLVYAG SG + 
Sbjct: 301 LANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEG 360

Query: 381 SEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNAGGAAMILINQKPNGFTTSADA 440
           SE+CV+GSL  L+V GK+VVCE GG IGR+ KGLVVKN GGAAMIL+NQKP+GF+T A+A
Sbjct: 361 SEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEA 420

Query: 441 HVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGGSNTDFSPAMASFSSRGPCLSS 500
           HVLP T +SYE GLKIK YI+SS NP ASISF+GT++G   T FSPAMASFSSRGPC +S
Sbjct: 421 HVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQAS 480

Query: 501 HGILKPDITGPGVNILAAWPFPI--DSNKNTKSTFNVMSGTSMSCPHLSGIAALIKNVHP 560
            GILKPDITGPGVNILAAWPFP+  ++N NTKSTFNV+SGTSMSCPHLSGIAALIK+ HP
Sbjct: 481 PGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHP 540

Query: 561 NWSPAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFATGSGHVNPSKAADPGLVYDIQAD 620
           NWSPAAIKSAIMTSADV+ PQGKPIVDQDLKPANFFA GSGHVNPSKAA+PGLVYDIQ D
Sbjct: 541 NWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPD 600

Query: 621 DYVRYLCGLYTDVQVSMIVRRQITCSTVSKIREGDLNYPSFSVTLGA-SQTFSRTVTNVG 680
           DYV YLC LYTD QVS+IVRRQ+TCSTVS+IREGDLNYPSF+V+LGA SQ F+RTVTNVG
Sbjct: 601 DYVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVG 660

Query: 681 DANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVTFSVDALVKVTSEFSEGYLKWV 704
           DANSVY AIV+AP GVSVRVTP NLKFS LNEK+TYSVTFS    V+  SEFSEGYL WV
Sbjct: 661 DANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWV 720

BLAST of HG10009218 vs. ExPASy TrEMBL
Match: A0A5A7THE4 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003290 PE=3 SV=1)

HSP 1 Score: 1060.4 bits (2741), Expect = 3.2e-306
Identity = 551/733 (75.17%), Postives = 608/733 (82.95%), Query Frame = 0

Query: 21  MKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLESWYTSFLPKTIE-TSNEQSRLLYS 80
           MKT +S TSNT  LQTYIVHV+QP LE   DT DL++WYTSFLP+TIE +S+EQ RLLYS
Sbjct: 1   MKTELSSTSNT-NLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYS 60

Query: 81  YRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNF 140
           YRHVMSGFSARLT+EQVK+MEEKDGFISA PET LNLHTTHTPEYLGLN  FGLWK+SNF
Sbjct: 61  YRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNF 120

Query: 141 GKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNLANTVL 200
           GKGVIIGVLDTGI+P+HPSFND+GM  PPAKWKGRCEFGASICNNKLIGARTFNLAN  +
Sbjct: 121 GKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFV 180

Query: 201 MGETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGIAPLAHVAMYKVCS-KGCQSS 260
           +G++P DENGHGTHTASTAAGTFV+GAEALGNARGKAVG+APLAH+AMYKVCS  GC SS
Sbjct: 181 IGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSS 240

Query: 261 DILAGLDAAIDDGVDVLSISIGSGS----------------------------------- 320
           DILA LDAAIDDGVDVLS+S+G+ S                                   
Sbjct: 241 DILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNT 300

Query: 321 ----------VGASTIDRTIAALAKLGNDEVHVGESLFQPRDFTSTFLPLVYAGNSGTKR 380
                     VGASTIDR I ALAKLG+ EV+ GESLFQPR+F S FLPLVYAG SG + 
Sbjct: 301 LANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEG 360

Query: 381 SEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNAGGAAMILINQKPNGFTTSADA 440
           SE+C+QGSL  L+V GKVVVCE GG I RV KGLVVKN GGAAMILINQKP GF+T A+A
Sbjct: 361 SEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEA 420

Query: 441 HVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGGSNTDFSPAMASFSSRGPCLSS 500
           HVLP T +SYE GLKIKAYI+SS NP ASISFKGT++G   T FSPAMASFSSRGPC +S
Sbjct: 421 HVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQAS 480

Query: 501 HGILKPDITGPGVNILAAWPFPI--DSNKNTKSTFNVMSGTSMSCPHLSGIAALIKNVHP 560
            GILKPDITGPGVNILAAWPFP+  ++N NTKSTFNV+SGTSMSCPHLSGIAALIK+ HP
Sbjct: 481 PGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHP 540

Query: 561 NWSPAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFATGSGHVNPSKAADPGLVYDIQAD 620
           NWSPAAIKSAIMTSADV+ PQGKPI+DQDLKPANFFA GSGHVNPSKAA+PGLVYDIQ D
Sbjct: 541 NWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPD 600

Query: 621 DYVRYLCGLYTDVQVSMIVRRQITCSTVSKIREGDLNYPSFSVTLGA-SQTFSRTVTNVG 680
           DY+ YLC LYTD QVS+IVR+Q+TCSTV +IREGDLNYPSF+V+L A SQTF+RTVTNVG
Sbjct: 601 DYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVG 660

Query: 681 DANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVTFSVDALVKVTSEFSEGYLKWV 704
           DANSVY  IVEAP GVSV+VTPSNLKFS LNEK+TYSVTFS    V+ TSEFSEGYL WV
Sbjct: 661 DANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWV 720

BLAST of HG10009218 vs. ExPASy TrEMBL
Match: A0A5A7TMM4 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003280 PE=3 SV=1)

HSP 1 Score: 1003.4 bits (2593), Expect = 4.6e-289
Identity = 519/756 (68.65%), Postives = 600/756 (79.37%), Query Frame = 0

Query: 1   MVVLPLFCIFFLLNFHGNVAMKTNVSDTSNTIKLQTYIVHVQQPEL----ESSDDTFDLE 60
           MVVL    IFFLLN   +VA K  +  T    KLQTYIVHV+QP++    ESS+D   +E
Sbjct: 1   MVVLRFLSIFFLLNIESHVAAKVELPTTPLQSKLQTYIVHVEQPKIRLVGESSND--HIE 60

Query: 61  SWYTSFLPKTIETSNEQSRLLYSYRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNL 120
           SWY SF+PK+ ET+ EQ +LLYSYR+VMSGFSARLT EQVK+ME+KDGFISA PETI++L
Sbjct: 61  SWYMSFIPKSTETTVEQPQLLYSYRNVMSGFSARLTIEQVKAMEKKDGFISAMPETIMSL 120

Query: 121 HTTHTPEYLGLNQQFGLWKDSNFGKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCE 180
           HTTHTPEYLGLNQQFG+WK+SNFGKGVI+GVLDTGI+P+HPSFND+GM  PPAKWKG+CE
Sbjct: 121 HTTHTPEYLGLNQQFGIWKNSNFGKGVIVGVLDTGIHPNHPSFNDEGMSPPPAKWKGKCE 180

Query: 181 FGASICNNKLIGARTFNLANTVLMGETPIDENGHGTHTASTAAGTFVEGAEALGNARGKA 240
           F +S+CNNKLIGARTFNL N  LM E+P DE GHGTHTASTAAG FVE AEALGNA+GKA
Sbjct: 181 FNSSLCNNKLIGARTFNLGNNFLMEESPNDEKGHGTHTASTAAGAFVEDAEALGNAKGKA 240

Query: 241 VGIAPLAHVAMYKVCS-KGCQSSDILAGLDAAIDDGVDVLSISIGSGS------------ 300
            GIAPLAH+A+YKVCS K C SSD+ AG+DAAIDDGVDVLSIS+GS S            
Sbjct: 241 AGIAPLAHLAIYKVCSGKRCPSSDVFAGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVAT 300

Query: 301 ---------------------------------VGASTIDRTIAALAKLGNDEVHVGESL 360
                                            VGASTI+R I A+AKLGN E + GESL
Sbjct: 301 FGAIQKGIFVSSSAGNSGPLNSTLSNDAPWILTVGASTINRRIVAVAKLGNGEQYEGESL 360

Query: 361 FQPRDFTSTFLPLVYAGNSGTKRSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVK 420
           +QP DF S FLPLVYAGN   K   FC +GSL N+DVKGKVVVCE  G +GRV KGLVVK
Sbjct: 361 YQPNDFPSKFLPLVYAGNRENKTYAFCGEGSLENMDVKGKVVVCEGKGGVGRVAKGLVVK 420

Query: 421 NAGGAAMILINQKPNGFTTSADAHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTII 480
           NAGGAAMILINQ+ +GF+T ++AHVLPAT VSY+AG+ IK+YI+SSQNPTASISFKGT+I
Sbjct: 421 NAGGAAMILINQEEDGFSTLSEAHVLPATHVSYKAGVLIKSYINSSQNPTASISFKGTVI 480

Query: 481 GGSNTDFSPAMASFSSRGPCLSSHGILKPDITGPGVNILAAWPFPIDSNKNTKSTFNVMS 540
           G  +   +P+MASFSSRGPCL S GILKPDITGPGVNILAAWPFP+D++ NTKSTFNV+S
Sbjct: 481 GDGDDFSAPSMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDNDTNTKSTFNVIS 540

Query: 541 GTSMSCPHLSGIAALIKNVHPNWSPAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFATG 600
           GTSMSCPHLSGIAALIK+VHPNWSPAAIKSAIMT+A++KTPQG+PIVDQDL+PANFFA G
Sbjct: 541 GTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTANIKTPQGEPIVDQDLQPANFFAMG 600

Query: 601 SGHVNPSKAADPGLVYDIQADDYVRYLCGLYTDVQVSMIVRRQITCSTVSKIREGDLNYP 660
           +GHVNPSKAADPGLVYDIQ DDY+ YLCGLY D +VS+IV R + C  V  IREGDLNYP
Sbjct: 601 AGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDEEVSIIVHRTVICGLVPSIREGDLNYP 660

Query: 661 SFSVTLGASQTFSRTVTNVGDANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVTF 705
           SFSV LG  QTF RTVTNVG+ANSVY+AIVEAP+GVS+ V P  L FS +N+ +T++VTF
Sbjct: 661 SFSVALGGLQTFKRTVTNVGEANSVYTAIVEAPLGVSMTVMPRKLFFSRVNQTMTFTVTF 720

BLAST of HG10009218 vs. ExPASy TrEMBL
Match: A0A1S3AUP1 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103483066 PE=3 SV=1)

HSP 1 Score: 996.5 bits (2575), Expect = 5.6e-287
Identity = 515/752 (68.48%), Postives = 597/752 (79.39%), Query Frame = 0

Query: 5   PLFCIFFLLNFHGNVAMKTNVSDTSNTIKLQTYIVHVQQPEL----ESSDDTFDLESWYT 64
           PL+  FFLLN   +VA K  +  T    KLQTYIVHV+QP++    ESS+D   +ESWY 
Sbjct: 16  PLY--FFLLNIESHVAAKVELPTTPLQSKLQTYIVHVEQPKIRLVGESSND--HIESWYM 75

Query: 65  SFLPKTIETSNEQSRLLYSYRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTTH 124
           SF+PK+ ET+ EQ +LLYSYR+VMSGFSARLT EQVK+ME+KDGFISA PETI++LHTTH
Sbjct: 76  SFIPKSTETTVEQPQLLYSYRNVMSGFSARLTIEQVKAMEKKDGFISAMPETIMSLHTTH 135

Query: 125 TPEYLGLNQQFGLWKDSNFGKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCEFGAS 184
           TPEYLGLNQQFG+WK+SNFGKGVI+GVLDTGI+P+HPSFND+GM  PPAKWKG+CEF +S
Sbjct: 136 TPEYLGLNQQFGIWKNSNFGKGVIVGVLDTGIHPNHPSFNDEGMSPPPAKWKGKCEFNSS 195

Query: 185 ICNNKLIGARTFNLANTVLMGETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGIA 244
           +CNNKLIGARTFNL N  LM E+P DE GHGTHTASTAAG FVE AEALGNA+GKA GIA
Sbjct: 196 LCNNKLIGARTFNLGNNFLMEESPNDEKGHGTHTASTAAGAFVEDAEALGNAKGKAAGIA 255

Query: 245 PLAHVAMYKVCS-KGCQSSDILAGLDAAIDDGVDVLSISIGSGS---------------- 304
           PLAH+A+YKVCS K C SSD+ AG+DAAIDDGVDVLSIS+GS S                
Sbjct: 256 PLAHLAIYKVCSGKRCPSSDVFAGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVATFGAI 315

Query: 305 -----------------------------VGASTIDRTIAALAKLGNDEVHVGESLFQPR 364
                                        VGASTI+R I A+AKLGN E + GESL+QP 
Sbjct: 316 QKGIFVSSSAGNSGPLNSTLSNDAPWILTVGASTINRRIVAVAKLGNGEQYEGESLYQPN 375

Query: 365 DFTSTFLPLVYAGNSGTKRSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNAGG 424
           DF S FLPLVYAGN   K   FC +GSL N+DVKGKVVVCE  G +GRV KGLVVKNAGG
Sbjct: 376 DFPSKFLPLVYAGNRENKTYAFCGEGSLENMDVKGKVVVCEGKGGVGRVAKGLVVKNAGG 435

Query: 425 AAMILINQKPNGFTTSADAHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGGSN 484
           AAMILINQ+ +GF+T ++AHVLPAT VSY+AG+ IK+YI+SSQNPTASISFKGT+IG  +
Sbjct: 436 AAMILINQEEDGFSTLSEAHVLPATHVSYKAGVLIKSYINSSQNPTASISFKGTVIGDGD 495

Query: 485 TDFSPAMASFSSRGPCLSSHGILKPDITGPGVNILAAWPFPIDSNKNTKSTFNVMSGTSM 544
              +P+MASFSSRGPCL S GILKPDITGPGVNILAAWPFP+D++ NTKSTFNV+SGTSM
Sbjct: 496 DFSAPSMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDNDTNTKSTFNVISGTSM 555

Query: 545 SCPHLSGIAALIKNVHPNWSPAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFATGSGHV 604
           SCPHLSGIAALIK+VHPNWSPAAIKSAIMT+A++KTPQG+PI DQDL+PANFFA G+GHV
Sbjct: 556 SCPHLSGIAALIKSVHPNWSPAAIKSAIMTTANIKTPQGEPITDQDLQPANFFAMGAGHV 615

Query: 605 NPSKAADPGLVYDIQADDYVRYLCGLYTDVQVSMIVRRQITCSTVSKIREGDLNYPSFSV 664
           NPSKAADPGLVYDIQ DDY+ YLCGLY D +VS+IV R + C  V  IREGDLNYPSFSV
Sbjct: 616 NPSKAADPGLVYDIQPDDYIPYLCGLYKDEEVSIIVHRTVICGLVLSIREGDLNYPSFSV 675

Query: 665 TLGASQTFSRTVTNVGDANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVTFSVDA 705
            LG  QTF RTVTNVG+ANSVY+AIVEAP+GVS+ V P  L FS +N+ +T++VTF+   
Sbjct: 676 ALGGLQTFKRTVTNVGEANSVYTAIVEAPLGVSMTVMPRKLFFSRVNQTMTFTVTFNRIR 735

BLAST of HG10009218 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 530.0 bits (1364), Expect = 2.9e-150
Identity = 328/765 (42.88%), Postives = 446/765 (58.30%), Query Frame = 0

Query: 10  FFLLNFHGNVAMKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLES-WYTSFLPKTIE 69
           FFLL   G   + ++ SD        TYIVH+ + ++ SS   FDL S WY S    ++ 
Sbjct: 11  FFLLLCLGFCHVSSSSSDQG------TYIVHMAKSQMPSS---FDLHSNWYDS----SLR 70

Query: 70  TSNEQSRLLYSYRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTTHTPEYLGLN 129
           + ++ + LLY+Y + + GFS RLT+E+  S+  + G IS  PE    LHTT TP +LGL+
Sbjct: 71  SISDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLD 130

Query: 130 QQFG-LWKDSNFGKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCE----FGASICN 189
           +    L+ ++     V++GVLDTG+ P+  S++D+G    P+ WKG CE    F AS+CN
Sbjct: 131 EHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCN 190

Query: 190 NKLIGARTFNLANTVLMG--------ETPIDENGHGTHTASTAAGTFVEGAEALGNARGK 249
            KLIGAR F       MG         +P D++GHGTHT+STAAG+ VEGA  LG A G 
Sbjct: 191 RKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGT 250

Query: 250 AVGIAPLAHVAMYKVC-SKGCQSSDILAGLDAAIDDGVDVLSISIGSG------------ 309
           A G+AP A VA+YKVC   GC SSDILA +D AI D V+VLS+S+G G            
Sbjct: 251 ARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIG 310

Query: 310 ---------------------------------SVGASTIDRTIAALAKLGNDEVHVGES 369
                                            +VGA T+DR   ALA LGN +   G S
Sbjct: 311 AFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVS 370

Query: 370 LFQPRDFTSTFLPLVYAGN-SGTKRSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLV 429
           LF+        LP +YAGN S       C+ G+L    VKGK+V+C+ G N  RV+KG V
Sbjct: 371 LFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGIN-ARVQKGDV 430

Query: 430 VKNAGGAAMILINQKPNGFTTSADAHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGT 489
           VK AGG  MIL N   NG    ADAH+LPAT V  +AG  I+ Y+++  NPTASIS  GT
Sbjct: 431 VKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGT 490

Query: 490 IIGGSNTDFSPAMASFSSRGPCLSSHGILKPDITGPGVNILAAW-----PFPIDSNKNTK 549
           ++G      SP +A+FSSRGP   +  ILKPD+  PGVNILAAW     P  + S+ + +
Sbjct: 491 VVG---VKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASD-SRR 550

Query: 550 STFNVMSGTSMSCPHLSGIAALIKNVHPNWSPAAIKSAIMTSADVKTPQGKPIVD-QDLK 609
             FN++SGTSMSCPH+SG+AAL+K+VHP WSPAAI+SA+MT+A      GKP++D    K
Sbjct: 551 VEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGK 610

Query: 610 PANFFATGSGHVNPSKAADPGLVYDIQADDYVRYLCGL-YTDVQVSMIVRRQITCSTVSK 669
           P+  F  G+GHV+P+ A +PGL+YD+  +DY+ +LC L YT  Q+  + RR  TC     
Sbjct: 611 PSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKS 670

Query: 670 IREGDLNYPSFSVTLG--ASQTFSRTVTNVGDANSVYSAIVEAPVGVSVRVTPSNLKFSS 705
               DLNYPSF+V +    +  ++RTVT+VG A +    +     GV + V P+ L F  
Sbjct: 671 YSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKE 730

BLAST of HG10009218 vs. TAIR 10
Match: AT1G04110.1 (Subtilase family protein )

HSP 1 Score: 526.2 bits (1354), Expect = 4.1e-149
Identity = 330/789 (41.83%), Postives = 449/789 (56.91%), Query Frame = 0

Query: 5   PLF-CIFFLLNFHGNVAMKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLESWYTSFL 64
           P F CI FLL            S +S  ++ QTYIV +  P  E++        W+ SFL
Sbjct: 5   PFFLCIIFLL----------FCSSSSEILQKQTYIVQL-HPNSETAKTFASKFDWHLSFL 64

Query: 65  PKTI-----ETSNEQSRLLYSYRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHT 124
            + +     E     SRLLYSY   + GF+A+LTE + + +      ++ RP+ +L + T
Sbjct: 65  QEAVLGVEEEEEEPSSRLLYSYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDHVLQVQT 124

Query: 125 THTPEYLGLN--QQFGLWKDSNFGKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCE 184
           T++ ++LGL+     G+W  S FG+G IIGVLDTG+ P+ PSF+D GMP  P KWKG C+
Sbjct: 125 TYSYKFLGLDGFGNSGVWSKSRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRKWKGICQ 184

Query: 185 ----FGASICNNKLIGARTFNLANTVLMG--ETP---------IDENGHGTHTASTAAGT 244
               F +S CN KLIGAR F   + V     E+P          D  GHGTHTAST  G+
Sbjct: 185 EGESFSSSSCNRKLIGARFFIRGHRVANSPEESPNMPREYISARDSTGHGTHTASTVGGS 244

Query: 245 FVEGAEALGNARGKAVGIAPLAHVAMYKVC-SKGCQSSDILAGLDAAIDDGVDVLSISIG 304
            V  A  LGN  G A G+AP AH+A+YKVC   GC SSDILA +D AI D VDVLS+S+G
Sbjct: 245 SVSMANVLGNGAGVARGMAPGAHIAVYKVCWFNGCYSSDILAAIDVAIQDKVDVLSLSLG 304

Query: 305 S---------------------------------------------GSVGASTIDRTIAA 364
                                                          ++GA T+DR   A
Sbjct: 305 GFPIPLYDDTIAIGTFRAMERGISVICAAGNNGPIESSVANTAPWVSTIGAGTLDRRFPA 364

Query: 365 LAKLGNDEVHVGESLFQPRDFTST--FLPLVYAGNSGTKRSEFCVQGSLANLDVKGKVVV 424
           + +L N ++  GESL+  +   +    + ++Y    G K SEFC++GSL   +++GK+V+
Sbjct: 365 VVRLANGKLLYGESLYPGKGIKNAGREVEVIYV-TGGDKGSEFCLRGSLPREEIRGKMVI 424

Query: 425 CETGGNIGRVKKGLVVKNAGGAAMILINQKPNGFTTSADAHVLPATRVSYEAGLKIKAYI 484
           C+ G N GR +KG  VK AGG AMIL N + N    S D H+LPAT + Y   + +KAY+
Sbjct: 425 CDRGVN-GRSEKGEAVKEAGGVAMILANTEINQEEDSIDVHLLPATLIGYTESVLLKAYV 484

Query: 485 SSSQNPTASISFKGTIIGGSNTDFSPAMASFSSRGPCLSSHGILKPDITGPGVNILAAWP 544
           +++  P A I F GT+IG S    +P +A FS+RGP L++  ILKPD+  PGVNI+AAWP
Sbjct: 485 NATVKPKARIIFGGTVIGRSR---APEVAQFSARGPSLANPSILKPDMIAPGVNIIAAWP 544

Query: 545 -------FPIDSNKNTKSTFNVMSGTSMSCPHLSGIAALIKNVHPNWSPAAIKSAIMTSA 604
                   P DS    +  F VMSGTSMSCPH+SGI ALI++ +PNWSPAAIKSA+MT+A
Sbjct: 545 QNLGPTGLPYDSR---RVNFTVMSGTSMSCPHVSGITALIRSAYPNWSPAAIKSALMTTA 604

Query: 605 DVKTPQGKPIVDQDLKPANFFATGSGHVNPSKAADPGLVYDIQADDYVRYLCGL-YTDVQ 664
           D+   QGK I D + KPA  FA G+GHVNP KA +PGLVY+IQ  DY+ YLC L +T   
Sbjct: 605 DLYDRQGKAIKDGN-KPAGVFAIGAGHVNPQKAINPGLVYNIQPVDYITYLCTLGFTRSD 664

Query: 665 VSMIVRRQITCSTVSKIREG-DLNYPSFSVTLGASQT---FSRTVTNVGDANSVYSAIVE 706
           +  I  + ++C+ + +   G  LNYPS +V     +T    +R VTNVG  NS+YS  V+
Sbjct: 665 ILAITHKNVSCNGILRKNPGFSLNYPSIAVIFKRGKTTEMITRRVTNVGSPNSIYSVNVK 724

BLAST of HG10009218 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 510.4 bits (1313), Expect = 2.3e-144
Identity = 319/759 (42.03%), Postives = 436/759 (57.44%), Query Frame = 0

Query: 28  TSNTIKLQTYIVHVQ---QPELESSDDTFDLESWYTSFLPKTIETSNEQSRLLYSYRHVM 87
           +S++  L++YIVHVQ   +P L SS +     +W+ S L +++ +S + + LLYSY   +
Sbjct: 24  SSSSDGLESYIVHVQRSHKPSLFSSHN-----NWHVSLL-RSLPSSPQPATLLYSYSRAV 83

Query: 88  SGFSARLTEEQVKSMEEKDGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNFGKGVI 147
            GFSARL+  Q  ++      IS  P+    +HTTHTP +LG +Q  GLW +SN+G+ VI
Sbjct: 84  HGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGEDVI 143

Query: 148 IGVLDTGINPDHPSFNDDGMPQPPAKWKGRCEFG----ASICNNKLIGARTF-------- 207
           +GVLDTGI P+HPSF+D G+   P+ WKG CE G    AS CN KLIGAR F        
Sbjct: 144 VGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYLTQR 203

Query: 208 --NLANTVLMGETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGIAPLAHVAMYKV 267
                +      +P D  GHGTHTASTAAG+ V  A     ARG A G+A  A +A YK+
Sbjct: 204 NGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYKI 263

Query: 268 C-SKGCQSSDILAGLDAAIDDGVDVLSISIG-SGS------------------------- 327
           C + GC  SDILA +D A+ DGV V+S+S+G SGS                         
Sbjct: 264 CWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVSC 323

Query: 328 ---------------------VGASTIDRTIAALAKLGNDEVHVGESLFQPRDFTSTFLP 387
                                VGAST+DR  AA A  G+ +V  G SL+       + L 
Sbjct: 324 SAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDSQLS 383

Query: 388 LVYAGNSGTKRSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNAGGAAMILINQ 447
           LVY+G+ G   S  C  G L +  V+GK+V+C+ GGN  RV+KG  VK AGGA MIL N 
Sbjct: 384 LVYSGDCG---SRLCYPGKLNSSLVEGKIVLCDRGGN-ARVEKGSAVKLAGGAGMILANT 443

Query: 448 KPNGFTTSADAHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGGSNTDFSPAMA 507
             +G   +AD+H++PAT V  +AG +I+ YI +S +PTA ISF GT+IG S    SP +A
Sbjct: 444 AESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPP--SPRVA 503

Query: 508 SFSSRGPCLSSHGILKPDITGPGVNILAAW-----PFPIDSNKNTKSTFNVMSGTSMSCP 567
           +FSSRGP   +  ILKPD+  PGVNILA W     P  +D +   +  FN++SGTSMSCP
Sbjct: 504 AFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPR-RVQFNIISGTSMSCP 563

Query: 568 HLSGIAALIKNVHPNWSPAAIKSAIMTSADVKTPQGKPIVDQDL-KPANFFATGSGHVNP 627
           H+SG+AAL++  HP+WSPAAIKSA++T+A      G+PI D    K +N F  G+GHV+P
Sbjct: 564 HVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDP 623

Query: 628 SKAADPGLVYDIQADDYVRYLCGLYTDVQVSMIVRRQIT----CSTVSKIREGDLNYPSF 687
           +KA +PGLVYDI+  +YV +LC +  +    ++  +  T    C T      GDLNYPSF
Sbjct: 624 NKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPSF 683

Query: 688 SV---TLGASQTFSRTVTNVG-DANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSV 705
           SV   + G    + R V NVG + ++VY   V++P  V + V+PS L FS     + Y V
Sbjct: 684 SVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYEV 743

BLAST of HG10009218 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 498.0 bits (1281), Expect = 1.2e-140
Identity = 318/743 (42.80%), Postives = 421/743 (56.66%), Query Frame = 0

Query: 30  NTIKLQTYIVHVQQPELESSDDTFDLESWYTSFLPKTIETSNEQSRLLYSYRHVMSGFSA 89
           +T   +TYI+ V   +   S  T     WYTS L       N +S LLY+Y     GFSA
Sbjct: 23  HTTAKKTYIIRVNHSDKPESFLTH--HDWYTSQL-------NSESSLLYTYTTSFHGFSA 82

Query: 90  RLTEEQVKS-MEEKDGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNFGKGVIIGVL 149
            L   +  S +   +  +    + +  LHTT TPE+LGLN +FG+    +   GVIIGVL
Sbjct: 83  YLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVL 142

Query: 150 DTGINPDHPSFNDDGMPQPPAKWKGRCE----FGASICNNKLIGARTFNLANTVLMG--- 209
           DTG+ P+  SF+D  MP+ P+KWKG CE    F + +CN KLIGAR+F+    +  G   
Sbjct: 143 DTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGF 202

Query: 210 ------ETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGIAPLAHVAMYKVC-SKG 269
                  +P D +GHGTHT++TAAG+ V  A  LG A G A G+A  A VA YKVC S G
Sbjct: 203 SSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTG 262

Query: 270 CQSSDILAGLDAAIDDGVDVLSISIGSGS------------------------------- 329
           C  SDILA +D AI DGVDVLS+S+G GS                               
Sbjct: 263 CFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGP 322

Query: 330 --------------VGASTIDRTIAALAKLGNDEVHVGESLFQPRDFTSTFLPLVY-AGN 389
                         VGA T+DR   A A LGN +   G SL+      +  L LVY  GN
Sbjct: 323 TRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGN 382

Query: 390 SGTKRSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNAGGAAMILINQKPNGFT 449
           S +  S  C+ GSL +  V+GK+VVC+ G N  RV+KG VV++AGG  MI+ N   +G  
Sbjct: 383 SSS--SNLCLPGSLDSSIVRGKIVVCDRGVN-ARVEKGAVVRDAGGLGMIMANTAASGEE 442

Query: 450 TSADAHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGGSNTDFSPAMASFSSRG 509
             AD+H+LPA  V  + G  ++ Y+ S   PTA + FKGT++   +   SP +A+FSSRG
Sbjct: 443 LVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVL---DVKPSPVVAAFSSRG 502

Query: 510 PCLSSHGILKPDITGPGVNILAAWP---FPIDSNKNTKST-FNVMSGTSMSCPHLSGIAA 569
           P   +  ILKPD+ GPGVNILA W     P   +K+++ T FN+MSGTSMSCPH+SG+A 
Sbjct: 503 PNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAG 562

Query: 570 LIKNVHPNWSPAAIKSAIMTSADVKTPQGKPIVD-QDLKPANFFATGSGHVNPSKAADPG 629
           L+K  HP WSP+AIKSA+MT+A V      P+ D  D   +N +A GSGHV+P KA  PG
Sbjct: 563 LLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPG 622

Query: 630 LVYDIQADDYVRYLCGL-YT-DVQVSMIVRRQITCSTVSKIRE-GDLNYPSFSVTLGASQ 689
           LVYDI  ++Y+R+LC L YT D  V+++ R  + CS   K  + G LNYPSFSV  G  +
Sbjct: 623 LVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCS--KKFSDPGQLNYPSFSVLFGGKR 682

Query: 690 T--FSRTVTNVGDANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVTFSVDALVKV 702
              ++R VTNVG A+SVY   V     V + V PS L F S+ EK  Y+VTF     V +
Sbjct: 683 VVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSM 742

BLAST of HG10009218 vs. TAIR 10
Match: AT1G01900.1 (subtilase family protein )

HSP 1 Score: 481.9 bits (1239), Expect = 8.9e-136
Identity = 305/780 (39.10%), Postives = 437/780 (56.03%), Query Frame = 0

Query: 1   MVVLPLFCIFFLLNFHGNVAMKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLESWYT 60
           M+    F +FF L F       +NVS      + QTY++H       ++     + S + 
Sbjct: 18  MMFFRSFIVFFFLIF-----FASNVSS-----RKQTYVIHT-----VTTSTKHIVTSLFN 77

Query: 61  SFLPKTIETSN-EQSRLLYSYRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTT 120
           S   + I   +     + Y Y + MSGFSA LT++Q+ +++   GFISA P+ +L+LHTT
Sbjct: 78  SLQTENINDDDFSLPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTT 137

Query: 121 HTPEYLGLNQQFGLWKDSNFGKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCE--- 180
           ++ E+LGL    GLW +++    VIIG++DTGI+P+H SF D  M   P++W+G C+   
Sbjct: 138 YSHEFLGLEFGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGT 197

Query: 181 -FGASICNNKLIGARTFNLANTVLMGE--------TPIDENGHGTHTASTAAGTFVEGAE 240
            F +S CN K+IGA  F      ++G+        +  D  GHGTHTASTAAG  V  A 
Sbjct: 198 NFSSSECNKKIIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKAN 257

Query: 241 ALGNARGKAVGIAPLAHVAMYKVC-SKGCQSSDILAGLDAAIDDGVDVLSISIGSGS--- 300
             G A+G A G+   + +A YK C + GC S+D++A +D AI DGVDV+S+S+G  S   
Sbjct: 258 YFGQAKGLASGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPF 317

Query: 301 ------------------------------------------VGASTIDRTIAALAKLGN 360
                                                     V AS  DRT  A+ ++GN
Sbjct: 318 YVDPIAIAGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGN 377

Query: 361 DEVHVGESLFQPRDFTSTFLPLVYAGNSGTKR-SEFCVQGSLANLDVKGKVVVCETGGNI 420
            +  VG SL++ +   +  LPL +   +G +  + FC++ SL    V+GK+V+C  G + 
Sbjct: 378 RKSLVGSSLYKGKSLKN--LPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGAS- 437

Query: 421 GRVKKGLVVKNAGGAAMILINQKPNGFTTSADAHVLPATRVSYEAGLKIKAYISSSQNPT 480
           GR  KG  VK +GGAAM+L++ +  G    AD HVLPA  + +  G  +  Y++ + N T
Sbjct: 438 GRTAKGEEVKRSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGAANAT 497

Query: 481 ASISFKGTIIGGSNTDFSPAMASFSSRGPCLSSHGILKPDITGPGVNILAAW-PFPIDS- 540
           AS+ F+GT  G +    +P +A+FSSRGP ++   I KPDI  PG+NILA W PF   S 
Sbjct: 498 ASVRFRGTAYGAT----APMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSL 557

Query: 541 --NKNTKSTFNVMSGTSMSCPHLSGIAALIKNVHPNWSPAAIKSAIMTSADVKTPQGKPI 600
             +   +  FN++SGTSM+CPH+SGIAALIK+VH +WSPA IKSAIMT+A +   + +PI
Sbjct: 558 LRSDPRRVQFNIISGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPI 617

Query: 601 VDQDL----KPANFFATGSGHVNPSKAADPGLVYDIQADDYVRYLCGL-YTDVQVSMIVR 660
            D+        A  FA G+G+V+P++A DPGLVYD    DY+ YLC L YT  ++ +   
Sbjct: 618 GDRGAAGAESAATAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSG 677

Query: 661 RQITC-STVSKIREGDLNYPSFSVTL--GA---SQTFSRTVTNVGDANSVYSAIVEAPVG 706
              TC S    +  GDLNYPSF+V L  GA   +  + RTVTNVG     Y   VE P G
Sbjct: 678 TNYTCASNAVVLSPGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKG 737