HG10009174 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10009174
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionheat stress transcription factor B-2b
LocationChr06: 3174481 .. 3175654 (+)
RNA-Seq ExpressionHG10009174
SyntenyHG10009174
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGCCGTCGCCGGCGGAAACGATCGGCGATTCTGGAACAGGAGATTCTCAGAGATCTATACCGACGCCATTTCTTACGAAAACTTATCAACTGGTTGATGATCCGGCTGTGGACGATCTCATCTCGTGGAATGAAGATGGATCTACCTTCATAGTTTGGCGACCGGCTGAATTTGCTCGAGATTTACTTCCTAAATACTTTAAACACAATAATTTCTCTAGTTTCGTCCGTCAACTCAACACTTACGTAAGTTTCTGCCGTTTTGAACTGTTTGCCAAATTTTCTATTTCTTGGGAACAATGGATGATGATGCCTCTGTTTAAGTACTTTCTCCAATATATTTCTCTCTGGAATCTTCGCTTTATTTTTGTTTTTTCAAAATACCGCTTAGGGATTCCGAAAGGTTGTGCCGGACCGATGGGAATTTGCGAATGATTGCTTCCGGAGAGGCGAGAAAGGACTTCTCCGAGACATTCAGCGGCGGAAAGTAGCGCTGTCGGTAACAACAACGTCGCCGGCGTCGCCAGCTGCGGTGGCTGTGCCTGTGACGGTGGTGGCGTCTCCAGCAGTGTTGGCTCACGTGATATCGCCGGCGAACTCTGCGGAAGAGCAGGTTACATCCTCGAACTCATCGCCAATGGCATTTCAGCGAAGTACGAGCTGCACCACGACGCCGGAACTTGTGAGAGAGAATGAGCGGTTAAGGAAGGAGAATATGCAACTGAGTCACGAGTTGACTCAGTTGAAAGGACTCTGTAACAACATACTATCGTTGATGACGAATTACGCTTCAGGTCACCATCACCAGTTGGAGTCGGTGAGCGTCCGAGACGGGAAGGCACTGGAGCTCTTACCGGCGAGGCAGGTGATGGAAGACGAAGGCGCCGTCAGCGACGGGGCTCATGAGGTGAGACTGAAGATGGAGGAGACCATGACAGCGGCCGCGGCGGCGGTAGGAATGACGCCGAAACTGTTCGGAGTATCGATCGGAGTGAAGCGGATGAGGAGAGATGAAGAAGACGAAGAAGAAGAGATGGTGGGGCAGAATCATGTACAGTCGGAAGAAGGTGAGACCGGGTCAGAGATCAAAGCTGAACCGTTGGATGAAAACTCTGAAAATCCAGAGGGATCCGCGTCGCCGTGGCTTGAACTCGGAAATCAAGGCTCCTGA

mRNA sequence

ATGACGCCGTCGCCGGCGGAAACGATCGGCGATTCTGGAACAGGAGATTCTCAGAGATCTATACCGACGCCATTTCTTACGAAAACTTATCAACTGGTTGATGATCCGGCTGTGGACGATCTCATCTCGTGGAATGAAGATGGATCTACCTTCATAGTTTGGCGACCGGCTGAATTTGCTCGAGATTTACTTCCTAAATACTTTAAACACAATAATTTCTCTAGTTTCGTCCGTCAACTCAACACTTACGGATTCCGAAAGGTTGTGCCGGACCGATGGGAATTTGCGAATGATTGCTTCCGGAGAGGCGAGAAAGGACTTCTCCGAGACATTCAGCGGCGGAAAGTAGCGCTGTCGGTAACAACAACGTCGCCGGCGTCGCCAGCTGCGGTGGCTGTGCCTGTGACGGTGGTGGCGTCTCCAGCAGTGTTGGCTCACGTGATATCGCCGGCGAACTCTGCGGAAGAGCAGGTTACATCCTCGAACTCATCGCCAATGGCATTTCAGCGAAGTACGAGCTGCACCACGACGCCGGAACTTGTGAGAGAGAATGAGCGGTTAAGGAAGGAGAATATGCAACTGAGTCACGAGTTGACTCAGTTGAAAGGACTCTGTAACAACATACTATCGTTGATGACGAATTACGCTTCAGGTCACCATCACCAGTTGGAGTCGGTGAGCGTCCGAGACGGGAAGGCACTGGAGCTCTTACCGGCGAGGCAGGTGATGGAAGACGAAGGCGCCGTCAGCGACGGGGCTCATGAGGTGAGACTGAAGATGGAGGAGACCATGACAGCGGCCGCGGCGGCGGTAGGAATGACGCCGAAACTGTTCGGAGTATCGATCGGAGTGAAGCGGATGAGGAGAGATGAAGAAGACGAAGAAGAAGAGATGGTGGGGCAGAATCATGTACAGTCGGAAGAAGGTGAGACCGGGTCAGAGATCAAAGCTGAACCGTTGGATGAAAACTCTGAAAATCCAGAGGGATCCGCGTCGCCGTGGCTTGAACTCGGAAATCAAGGCTCCTGA

Coding sequence (CDS)

ATGACGCCGTCGCCGGCGGAAACGATCGGCGATTCTGGAACAGGAGATTCTCAGAGATCTATACCGACGCCATTTCTTACGAAAACTTATCAACTGGTTGATGATCCGGCTGTGGACGATCTCATCTCGTGGAATGAAGATGGATCTACCTTCATAGTTTGGCGACCGGCTGAATTTGCTCGAGATTTACTTCCTAAATACTTTAAACACAATAATTTCTCTAGTTTCGTCCGTCAACTCAACACTTACGGATTCCGAAAGGTTGTGCCGGACCGATGGGAATTTGCGAATGATTGCTTCCGGAGAGGCGAGAAAGGACTTCTCCGAGACATTCAGCGGCGGAAAGTAGCGCTGTCGGTAACAACAACGTCGCCGGCGTCGCCAGCTGCGGTGGCTGTGCCTGTGACGGTGGTGGCGTCTCCAGCAGTGTTGGCTCACGTGATATCGCCGGCGAACTCTGCGGAAGAGCAGGTTACATCCTCGAACTCATCGCCAATGGCATTTCAGCGAAGTACGAGCTGCACCACGACGCCGGAACTTGTGAGAGAGAATGAGCGGTTAAGGAAGGAGAATATGCAACTGAGTCACGAGTTGACTCAGTTGAAAGGACTCTGTAACAACATACTATCGTTGATGACGAATTACGCTTCAGGTCACCATCACCAGTTGGAGTCGGTGAGCGTCCGAGACGGGAAGGCACTGGAGCTCTTACCGGCGAGGCAGGTGATGGAAGACGAAGGCGCCGTCAGCGACGGGGCTCATGAGGTGAGACTGAAGATGGAGGAGACCATGACAGCGGCCGCGGCGGCGGTAGGAATGACGCCGAAACTGTTCGGAGTATCGATCGGAGTGAAGCGGATGAGGAGAGATGAAGAAGACGAAGAAGAAGAGATGGTGGGGCAGAATCATGTACAGTCGGAAGAAGGTGAGACCGGGTCAGAGATCAAAGCTGAACCGTTGGATGAAAACTCTGAAAATCCAGAGGGATCCGCGTCGCCGTGGCTTGAACTCGGAAATCAAGGCTCCTGA

Protein sequence

MTPSPAETIGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFARDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVALSVTTTSPASPAAVAVPVTVVASPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPELVRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHHHQLESVSVRDGKALELLPARQVMEDEGAVSDGAHEVRLKMEETMTAAAAAVGMTPKLFGVSIGVKRMRRDEEDEEEEMVGQNHVQSEEGETGSEIKAEPLDENSENPEGSASPWLELGNQGS
Homology
BLAST of HG10009174 vs. NCBI nr
Match: XP_004143930.1 (heat stress transcription factor B-2b [Cucumis sativus] >XP_031741133.1 heat stress transcription factor B-2b [Cucumis sativus])

HSP 1 Score: 616.7 bits (1589), Expect = 1.2e-172
Identity = 322/342 (94.15%), Postives = 329/342 (96.20%), Query Frame = 0

Query: 1   MTPSPAETIGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA 60
           M PSPAE IGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA
Sbjct: 1   MAPSPAEPIGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA 60

Query: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVALSV 120
           RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFR+GEKGLLRDIQRRKV LSV
Sbjct: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRKGEKGLLRDIQRRKVVLSV 120

Query: 121 TTTSPASPAAVAVPVTVVASPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPEL 180
           TTT+  S AAVAVPVTV  SPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPEL
Sbjct: 121 TTTTTTS-AAVAVPVTVATSPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPEL 180

Query: 181 VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHHHQLESVSVRDGKALELLPAR 240
           VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASG H QLES SVRDGKALELLPAR
Sbjct: 181 VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGQHQQLESGSVRDGKALELLPAR 240

Query: 241 QVMEDEGAVSDGAHEVRLKMEETMTAAAAAVGMTPKLFGVSIGVKRMRRDEEDEEEEMVG 300
           QVMEDEGAVSDGAHEVRLKMEE MTAAAAAVGMTPKLFGVSIG+KRMRR+ E+EEEEMVG
Sbjct: 241 QVMEDEGAVSDGAHEVRLKMEEKMTAAAAAVGMTPKLFGVSIGMKRMRREIEEEEEEMVG 300

Query: 301 QNHVQSEEGETGSEIKAEPLDENSENPEGSASPWLELGNQGS 343
           QNHVQSEEGETGSEIKAEPLDENSE+P+GSASPWLELGNQGS
Sbjct: 301 QNHVQSEEGETGSEIKAEPLDENSEHPDGSASPWLELGNQGS 341

BLAST of HG10009174 vs. NCBI nr
Match: KAE8647868.1 (hypothetical protein Csa_000645 [Cucumis sativus])

HSP 1 Score: 616.7 bits (1589), Expect = 1.2e-172
Identity = 322/342 (94.15%), Postives = 329/342 (96.20%), Query Frame = 0

Query: 1   MTPSPAETIGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA 60
           M PSPAE IGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA
Sbjct: 389 MAPSPAEPIGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA 448

Query: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVALSV 120
           RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFR+GEKGLLRDIQRRKV LSV
Sbjct: 449 RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRKGEKGLLRDIQRRKVVLSV 508

Query: 121 TTTSPASPAAVAVPVTVVASPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPEL 180
           TTT+  S AAVAVPVTV  SPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPEL
Sbjct: 509 TTTTTTS-AAVAVPVTVATSPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPEL 568

Query: 181 VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHHHQLESVSVRDGKALELLPAR 240
           VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASG H QLES SVRDGKALELLPAR
Sbjct: 569 VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGQHQQLESGSVRDGKALELLPAR 628

Query: 241 QVMEDEGAVSDGAHEVRLKMEETMTAAAAAVGMTPKLFGVSIGVKRMRRDEEDEEEEMVG 300
           QVMEDEGAVSDGAHEVRLKMEE MTAAAAAVGMTPKLFGVSIG+KRMRR+ E+EEEEMVG
Sbjct: 629 QVMEDEGAVSDGAHEVRLKMEEKMTAAAAAVGMTPKLFGVSIGMKRMRREIEEEEEEMVG 688

Query: 301 QNHVQSEEGETGSEIKAEPLDENSENPEGSASPWLELGNQGS 343
           QNHVQSEEGETGSEIKAEPLDENSE+P+GSASPWLELGNQGS
Sbjct: 689 QNHVQSEEGETGSEIKAEPLDENSEHPDGSASPWLELGNQGS 729

BLAST of HG10009174 vs. NCBI nr
Match: XP_038875590.1 (heat stress transcription factor B-2b [Benincasa hispida] >XP_038875591.1 heat stress transcription factor B-2b [Benincasa hispida])

HSP 1 Score: 616.3 bits (1588), Expect = 1.6e-172
Identity = 323/343 (94.17%), Postives = 329/343 (95.92%), Query Frame = 0

Query: 1   MTPSPAETIGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA 60
           M PSPAE IGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA
Sbjct: 1   MAPSPAEPIGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA 60

Query: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVALSV 120
           RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVALS+
Sbjct: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVALSL 120

Query: 121 -TTTSPASPAAVAVPVTVVASPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPE 180
            TTT+ A+PAAVAVPV V ASPAVLAHVISPANS EEQVTSSNSSPM FQR TSCTTTPE
Sbjct: 121 TTTTATATPAAVAVPVAVAASPAVLAHVISPANSGEEQVTSSNSSPMTFQRGTSCTTTPE 180

Query: 181 LVRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHHHQLESVSVRDGKALELLPA 240
           LVRENERLRKENMQLSHELTQLKGLCNNILSLMTNYAS H+HQLESVSVRDGKALELLPA
Sbjct: 181 LVRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASDHNHQLESVSVRDGKALELLPA 240

Query: 241 RQVMEDEGAVSDGAHEVRLKMEETMTAAAAAVGMTPKLFGVSIGVKRMRRDEEDEEEEMV 300
           RQVMEDEGAVSDGA EVRLKMEETM   AAAVGMTPKLFGVSIGVKRMRR+E+DEEEEMV
Sbjct: 241 RQVMEDEGAVSDGAQEVRLKMEETM---AAAVGMTPKLFGVSIGVKRMRREEQDEEEEMV 300

Query: 301 GQNHVQSEEGETGSEIKAEPLDENSENPEGSASPWLELGNQGS 343
           GQNHVQSEEGETGSEIKAEPLDENSENPEGSASPWLELGNQGS
Sbjct: 301 GQNHVQSEEGETGSEIKAEPLDENSENPEGSASPWLELGNQGS 340

BLAST of HG10009174 vs. NCBI nr
Match: KAA0042789.1 (bifunctional UDP-glucose 4-epimerase and UDP-xylose 4-epimerase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 609.8 bits (1571), Expect = 1.5e-170
Identity = 319/342 (93.27%), Postives = 327/342 (95.61%), Query Frame = 0

Query: 1   MTPSPAETIGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA 60
           M PSPAE IGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA
Sbjct: 344 MAPSPAEPIGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA 403

Query: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVALSV 120
           RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFR+GEKGLLRDIQRRKVALSV
Sbjct: 404 RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRKGEKGLLRDIQRRKVALSV 463

Query: 121 TTTSPASPAAVAVPVTVVASPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPEL 180
           TTT+  + AAVAVPV V ASPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPEL
Sbjct: 464 TTTT--TSAAVAVPVPVAASPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPEL 523

Query: 181 VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHHHQLESVSVRDGKALELLPAR 240
           VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASG HH  ES SVRDGKALELLPAR
Sbjct: 524 VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGQHHHFESGSVRDGKALELLPAR 583

Query: 241 QVMEDEGAVSDGAHEVRLKMEETMTAAAAAVGMTPKLFGVSIGVKRMRRDEEDEEEEMVG 300
           QVMEDEGAVSDGA EVRLKMEE MTAAAAA G+TPKLFGVSIGVKRMRR+ E+EEEEMVG
Sbjct: 584 QVMEDEGAVSDGALEVRLKMEEKMTAAAAAAGVTPKLFGVSIGVKRMRREVEEEEEEMVG 643

Query: 301 QNHVQSEEGETGSEIKAEPLDENSENPEGSASPWLELGNQGS 343
           QNHVQSEEGETGSEIKAEPLDENSE+P+GSASPWLELGNQGS
Sbjct: 644 QNHVQSEEGETGSEIKAEPLDENSEHPDGSASPWLELGNQGS 683

BLAST of HG10009174 vs. NCBI nr
Match: XP_008437221.1 (PREDICTED: heat stress transcription factor B-2b [Cucumis melo])

HSP 1 Score: 600.9 bits (1548), Expect = 6.9e-168
Identity = 317/342 (92.69%), Postives = 325/342 (95.03%), Query Frame = 0

Query: 1   MTPSPAETIGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA 60
           M PSPAE IGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA
Sbjct: 1   MAPSPAEPIGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA 60

Query: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVALSV 120
           RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFR+GEKGLLRDIQRRKVALSV
Sbjct: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRKGEKGLLRDIQRRKVALSV 120

Query: 121 TTTSPASPAAVAVPVTVVASPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPEL 180
           TTT+  + AAVAVPV V ASPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPEL
Sbjct: 121 TTTT--TSAAVAVPVPVAASPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPEL 180

Query: 181 VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHHHQLESVSVRDGKALELLPAR 240
           VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASG HH  ES SVRDGKALELLPAR
Sbjct: 181 VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGQHHHFESGSVRDGKALELLPAR 240

Query: 241 QVMEDEGAVSDGAHEVRLKMEETMTAAAAAVGMTPKLFGVSIGVKRMRRDEEDEEEEMVG 300
           QVMEDEGAVSDGA EVRLKM E M AAAAA G+TPKLFGVSIGVKRMRR+ E+EEEEMVG
Sbjct: 241 QVMEDEGAVSDGALEVRLKMGEKM-AAAAAAGVTPKLFGVSIGVKRMRREVEEEEEEMVG 300

Query: 301 QNHVQSEEGETGSEIKAEPLDENSENPEGSASPWLELGNQGS 343
           QNHVQSEEGETGSEIKAEPLDENSE+P+GSASPWLELGNQGS
Sbjct: 301 QNHVQSEEGETGSEIKAEPLDENSEHPDGSASPWLELGNQGS 339

BLAST of HG10009174 vs. ExPASy Swiss-Prot
Match: Q9T0D3 (Heat stress transcription factor B-2b OS=Arabidopsis thaliana OX=3702 GN=HSFB2B PE=2 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 2.1e-95
Identity = 203/346 (58.67%), Postives = 243/346 (70.23%), Query Frame = 0

Query: 10  GDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFARDLLPKYFK 69
           G  G GDSQRSIPTPFLTKTYQLV+DP  D+LISWNEDG+TFIVWRPAEFARDLLPKYFK
Sbjct: 45  GGGGGGDSQRSIPTPFLTKTYQLVEDPVYDELISWNEDGTTFIVWRPAEFARDLLPKYFK 104

Query: 70  HNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVAL-SVTTTSPASP 129
           HNNFSSFVRQLNTYGFRKVVPDRWEF+NDCF+RGEK LLRDIQRRK++  ++   + A+ 
Sbjct: 105 HNNFSSFVRQLNTYGFRKVVPDRWEFSNDCFKRGEKILLRDIQRRKISQPAMAAAAAAAA 164

Query: 130 AAVAVPVTVVASPAVLAHVISPANSAEEQVTSSNSSPMA-------------FQRSTSCT 189
           AAVA     VA+  V+AH++SP+NS EEQV SSNSSP A              QR+TSCT
Sbjct: 165 AAVAASAVTVAAVPVVAHIVSPSNSGEEQVISSNSSPAAAAAAIGGVVGGGSLQRTTSCT 224

Query: 190 TTPELVRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHHHQLESVSVRDGKALE 249
           T PELV ENERLRK+N +L  E+T+LKGL  NI +LM N+  G       +   +GK L+
Sbjct: 225 TAPELVEENERLRKDNERLRKEMTKLKGLYANIYTLMANFTPGQEDCAHLLP--EGKPLD 284

Query: 250 LLPARQVMEDEGAVSDGAHEVRLKMEETMTAAAAAVGMTPKLFGVSIGVKRMRRDEE--D 309
           LLP RQ M +    S+    + LK+ E          +TP+LFGVSIGVKR RR+EE   
Sbjct: 285 LLPERQEMSEAIMASEIETGIGLKLGE---------DLTPRLFGVSIGVKRARREEELGA 344

Query: 310 EEEEMVGQNHVQSEEGETGSEIKAEPLDE-NSENPEGSASPWLELG 339
            EEE   +    ++EGE  S++KAEP++E NS N  GS   WLELG
Sbjct: 345 AEEEDDDRREAAAQEGEQSSDVKAEPMEENNSGNHNGS---WLELG 376

BLAST of HG10009174 vs. ExPASy Swiss-Prot
Match: Q6Z9C8 (Heat stress transcription factor B-2b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB2B PE=2 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 4.5e-69
Identity = 165/335 (49.25%), Postives = 202/335 (60.30%), Query Frame = 0

Query: 3   PSPAETIGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFARD 62
           P+PA      G G  QR++PTPFLTKTYQLVDDPAVDD+ISWN+DGSTF+VWRPAEFARD
Sbjct: 27  PAPAAEAAGVGVGQQQRTVPTPFLTKTYQLVDDPAVDDVISWNDDGSTFVVWRPAEFARD 86

Query: 63  LLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVALSVTT 122
           LLPKYFKHNNFSSFVRQLNTYGFRK+VPDRWEFANDCFRRGE+ LL +I RRK    VT 
Sbjct: 87  LLPKYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDCFRRGERRLLCEIHRRK----VTP 146

Query: 123 TSPASPAAVAVPVTVVASPAVLAHVISPANSAEEQVTSSNSS---PMAFQRSTSCT---- 182
            +PA+  A       +A P       SP  S EEQV SS+SS   P+   ++ S +    
Sbjct: 147 PAPAATTAAVAAAIPMALPVTTTRDGSPVLSGEEQVISSSSSPEPPLVLPQAPSGSGSGG 206

Query: 183 -TTPELVRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHHHQLESVSVRDGKAL 242
             + ++  ENERLR+EN QL+ EL+Q++ LCNNIL LM+ YAS    QL++ +       
Sbjct: 207 VASGDVGDENERLRRENAQLARELSQMRKLCNNILLLMSKYAS--TQQLDAANASSAAGN 266

Query: 243 ELLPARQVMEDEGAVSDGAHEVRLKMEETMTAAAAAVG--------MTPKLFGVSIGVKR 302
                      E A       V   M     AA+AA          M+ KLFGVSIG KR
Sbjct: 267 NNNNNCSGESAEAATPLPLPAVLDLMPSCPGAASAAAPVSDNEEGMMSAKLFGVSIGRKR 326

Query: 303 MRRDEEDEEEEMVGQNHVQSEEGETGSEIKAEPLD 322
           MR D   +++                + +KAEP+D
Sbjct: 327 MRHDGGGDDDH--------------AATVKAEPMD 341

BLAST of HG10009174 vs. ExPASy Swiss-Prot
Match: Q652B0 (Heat stress transcription factor B-2c OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB2C PE=2 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 5.8e-69
Identity = 175/351 (49.86%), Postives = 217/351 (61.82%), Query Frame = 0

Query: 18  QRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFARDLLPKYFKHNNFSSFV 77
           QRS+PTPFLTKTYQLV+DPAVDD+ISWNEDGSTF+VWRPAEFARDLLPKYFKHNNFSSFV
Sbjct: 32  QRSLPTPFLTKTYQLVEDPAVDDVISWNEDGSTFVVWRPAEFARDLLPKYFKHNNFSSFV 91

Query: 78  RQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRK-VALSVTTTSPASP----AAVA 137
           RQLNTYGFRK+VPDRWEFANDCFRRGEK LL DI RRK VA +     P SP    AA A
Sbjct: 92  RQLNTYGFRKIVPDRWEFANDCFRRGEKRLLCDIHRRKVVAAAAAAPPPPSPGMATAAAA 151

Query: 138 V---PVTVVASPAVLAHVI----SPANSAEEQVTSSNSSPMAFQRSTS------------ 197
           V    VTV A+P  +A  +    SPA+S+EEQV SSNS      R  S            
Sbjct: 152 VASGAVTVAAAPIPMALPVTRAGSPAHSSEEQVLSSNSGSGEEHRQASGSGSAPGGGGGG 211

Query: 198 CTTTPELVRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHHHQ----LESVSVR 257
             +  ++  ENERLR+EN +L+ EL  +K LCNNIL LM+ YA+  H +    + S++  
Sbjct: 212 SASGGDMGEENERLRRENARLTRELGHMKKLCNNILLLMSKYAATQHVEGSAGISSIANC 271

Query: 258 DGKALELLPARQVMEDEGAVSDGAHEVRLKMEETMTAAAAAVGM--------TPKLFGVS 317
            G++ E +P    +    A+ D      +     +  AAAA G+        + +LFGVS
Sbjct: 272 SGESSEAVPPPPPLPP--AILD-----LMPSCPALATAAAAAGLAIDGEPDPSARLFGVS 331

Query: 318 IGVKRMRRDEEDEEEEMVGQNHVQSEEGETGSEIKAEPLDENSENPEGSAS 333
           IG+KR R D     +E  G    Q+E G  G+++K E  D +     G +S
Sbjct: 332 IGLKRTRDDAAAAADE-DGGGEDQAEHG--GADVKPEAADPHPAGGGGGSS 372

BLAST of HG10009174 vs. ExPASy Swiss-Prot
Match: Q9SCW4 (Heat stress transcription factor B-2a OS=Arabidopsis thaliana OX=3702 GN=HSFB2A PE=2 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.7e-60
Identity = 143/300 (47.67%), Postives = 195/300 (65.00%), Query Frame = 0

Query: 12  SGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFARDLLPKYFKHN 71
           +G   SQRSIPTPFLTKT+ LV+D ++DD+ISWNEDGS+FIVW P +FA+DLLPK+FKHN
Sbjct: 11  TGESSSQRSIPTPFLTKTFNLVEDSSIDDVISWNEDGSSFIVWNPTDFAKDLLPKHFKHN 70

Query: 72  NFSSFVRQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVALSVTTTSPASPAAV 131
           NFSSFVRQLNTYGF+KVVPDRWEF+ND F+RGEK LLR+IQRRK    +TTT       V
Sbjct: 71  NFSSFVRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRK----ITTTH----QTV 130

Query: 132 AVPVTVVASPAVLAHVISPANSAEEQVTSS--NSSPMAFQRSTSCTT-----TPELVREN 191
             P +   +  +   V+SP+NS E+   +   +SSP ++    + TT     + EL+ EN
Sbjct: 131 VAPSSEQRNQTM---VVSPSNSGEDNNNNQVMSSSPSSWYCHQTKTTGNGGLSVELLEEN 190

Query: 192 ERLRKENMQLSHELTQLKGLCNNILSLMTNY-ASGHHHQLESVSVRDGKALELLPARQVM 251
           E+LR +N+QL+ ELTQ+K +C+NI SLM+NY  S    +  S      + +E LPA++  
Sbjct: 191 EKLRSQNIQLNRELTQMKSICDNIYSLMSNYVGSQPTDRSYSPGGSSSQPMEFLPAKRFS 250

Query: 252 EDEGAVSDGAHEVRLKMEETMTAAAAAVGMTPKLFGVSIGVKRMRRD-EEDEEEEMVGQN 303
           E             +++EE   A       +P+LFGV IG+KR R +  + +   +VG+N
Sbjct: 251 E-------------MEIEEEEEA-------SPRLFGVPIGLKRTRSEGVQVKTTAVVGEN 279

BLAST of HG10009174 vs. ExPASy Swiss-Prot
Match: P22335 (Heat shock factor protein HSF24 OS=Solanum peruvianum OX=4082 GN=HSF24 PE=2 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 4.6e-50
Identity = 134/324 (41.36%), Postives = 188/324 (58.02%), Query Frame = 0

Query: 17  SQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFARDLLPKYFKHNNFSSF 76
           SQR+ P PFL KTYQLVDD A DD+ISWNE G+TF+VW+ AEFA+DLLPKYFKHNNFSSF
Sbjct: 2   SQRTAPAPFLLKTYQLVDDAATDDVISWNEIGTTFVVWKTAEFAKDLLPKYFKHNNFSSF 61

Query: 77  VRQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVALSVTTTSPASPAAVAVPVT 136
           VRQLNTYGFRK+VPD+WEFAN+ F+RG+K LL  I+RRK      T++PA   +VA   +
Sbjct: 62  VRQLNTYGFRKIVPDKWEFANENFKRGQKELLTAIRRRK----TVTSTPAGGKSVAAGAS 121

Query: 137 VVASPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTP-------ELVRENERLRK 196
                       SP NS ++  +SS SSP +  ++     TP       +L  ENE+L+K
Sbjct: 122 A-----------SPDNSGDDIGSSSTSSPDS--KNPGSVDTPGKLSQFTDLSDENEKLKK 181

Query: 197 ENMQLSHELTQLKGLCNNILSLMTNY---ASGHHHQLESVSVRDGKALELLPARQVMEDE 256
           +N  LS EL Q K  CN +++ ++ Y   A    +++ S     G +LE     +++++ 
Sbjct: 182 DNQMLSSELVQAKKQCNELVAFLSQYVKVAPDMINRIMSQGTPSGSSLE-----ELVKEV 241

Query: 257 GAVSD----GAHEVRLKMEETMTAAAAAVGMTPKLFGVSIGVKRMRRDEEDEEEEMVGQN 316
           G V D    G++      E+         G T KLFGV +  K+ +R  ++  E   G+ 
Sbjct: 242 GGVKDLEEQGSYNDNDDKEDD-----DEKGDTLKLFGVLLKEKKKKRGPDENIETCGGRG 298

Query: 317 HVQSEEGETGSEIK-AEPLDENSE 326
            +       G  +K + P  E+S+
Sbjct: 302 KMMKTVDYNGPWMKMSSPAGESSK 298

BLAST of HG10009174 vs. ExPASy TrEMBL
Match: A0A0A0KNZ8 (HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G155480 PE=3 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 5.9e-173
Identity = 322/342 (94.15%), Postives = 329/342 (96.20%), Query Frame = 0

Query: 1   MTPSPAETIGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA 60
           M PSPAE IGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA
Sbjct: 1   MAPSPAEPIGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA 60

Query: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVALSV 120
           RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFR+GEKGLLRDIQRRKV LSV
Sbjct: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRKGEKGLLRDIQRRKVVLSV 120

Query: 121 TTTSPASPAAVAVPVTVVASPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPEL 180
           TTT+  S AAVAVPVTV  SPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPEL
Sbjct: 121 TTTTTTS-AAVAVPVTVATSPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPEL 180

Query: 181 VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHHHQLESVSVRDGKALELLPAR 240
           VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASG H QLES SVRDGKALELLPAR
Sbjct: 181 VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGQHQQLESGSVRDGKALELLPAR 240

Query: 241 QVMEDEGAVSDGAHEVRLKMEETMTAAAAAVGMTPKLFGVSIGVKRMRRDEEDEEEEMVG 300
           QVMEDEGAVSDGAHEVRLKMEE MTAAAAAVGMTPKLFGVSIG+KRMRR+ E+EEEEMVG
Sbjct: 241 QVMEDEGAVSDGAHEVRLKMEEKMTAAAAAVGMTPKLFGVSIGMKRMRREIEEEEEEMVG 300

Query: 301 QNHVQSEEGETGSEIKAEPLDENSENPEGSASPWLELGNQGS 343
           QNHVQSEEGETGSEIKAEPLDENSE+P+GSASPWLELGNQGS
Sbjct: 301 QNHVQSEEGETGSEIKAEPLDENSEHPDGSASPWLELGNQGS 341

BLAST of HG10009174 vs. ExPASy TrEMBL
Match: A0A5A7TN46 (Bifunctional UDP-glucose 4-epimerase and UDP-xylose 4-epimerase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002920 PE=3 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 7.2e-171
Identity = 319/342 (93.27%), Postives = 327/342 (95.61%), Query Frame = 0

Query: 1   MTPSPAETIGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA 60
           M PSPAE IGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA
Sbjct: 344 MAPSPAEPIGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA 403

Query: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVALSV 120
           RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFR+GEKGLLRDIQRRKVALSV
Sbjct: 404 RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRKGEKGLLRDIQRRKVALSV 463

Query: 121 TTTSPASPAAVAVPVTVVASPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPEL 180
           TTT+  + AAVAVPV V ASPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPEL
Sbjct: 464 TTTT--TSAAVAVPVPVAASPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPEL 523

Query: 181 VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHHHQLESVSVRDGKALELLPAR 240
           VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASG HH  ES SVRDGKALELLPAR
Sbjct: 524 VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGQHHHFESGSVRDGKALELLPAR 583

Query: 241 QVMEDEGAVSDGAHEVRLKMEETMTAAAAAVGMTPKLFGVSIGVKRMRRDEEDEEEEMVG 300
           QVMEDEGAVSDGA EVRLKMEE MTAAAAA G+TPKLFGVSIGVKRMRR+ E+EEEEMVG
Sbjct: 584 QVMEDEGAVSDGALEVRLKMEEKMTAAAAAAGVTPKLFGVSIGVKRMRREVEEEEEEMVG 643

Query: 301 QNHVQSEEGETGSEIKAEPLDENSENPEGSASPWLELGNQGS 343
           QNHVQSEEGETGSEIKAEPLDENSE+P+GSASPWLELGNQGS
Sbjct: 644 QNHVQSEEGETGSEIKAEPLDENSEHPDGSASPWLELGNQGS 683

BLAST of HG10009174 vs. ExPASy TrEMBL
Match: A0A1S3AU16 (heat stress transcription factor B-2b OS=Cucumis melo OX=3656 GN=LOC103482716 PE=3 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 3.3e-168
Identity = 317/342 (92.69%), Postives = 325/342 (95.03%), Query Frame = 0

Query: 1   MTPSPAETIGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA 60
           M PSPAE IGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA
Sbjct: 1   MAPSPAEPIGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA 60

Query: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVALSV 120
           RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFR+GEKGLLRDIQRRKVALSV
Sbjct: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRKGEKGLLRDIQRRKVALSV 120

Query: 121 TTTSPASPAAVAVPVTVVASPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPEL 180
           TTT+  + AAVAVPV V ASPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPEL
Sbjct: 121 TTTT--TSAAVAVPVPVAASPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPEL 180

Query: 181 VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHHHQLESVSVRDGKALELLPAR 240
           VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASG HH  ES SVRDGKALELLPAR
Sbjct: 181 VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGQHHHFESGSVRDGKALELLPAR 240

Query: 241 QVMEDEGAVSDGAHEVRLKMEETMTAAAAAVGMTPKLFGVSIGVKRMRRDEEDEEEEMVG 300
           QVMEDEGAVSDGA EVRLKM E M AAAAA G+TPKLFGVSIGVKRMRR+ E+EEEEMVG
Sbjct: 241 QVMEDEGAVSDGALEVRLKMGEKM-AAAAAAGVTPKLFGVSIGVKRMRREVEEEEEEMVG 300

Query: 301 QNHVQSEEGETGSEIKAEPLDENSENPEGSASPWLELGNQGS 343
           QNHVQSEEGETGSEIKAEPLDENSE+P+GSASPWLELGNQGS
Sbjct: 301 QNHVQSEEGETGSEIKAEPLDENSEHPDGSASPWLELGNQGS 339

BLAST of HG10009174 vs. ExPASy TrEMBL
Match: A0A6J1DU11 (heat stress transcription factor B-2b OS=Momordica charantia OX=3673 GN=LOC111024372 PE=3 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 7.4e-160
Identity = 306/344 (88.95%), Postives = 321/344 (93.31%), Query Frame = 0

Query: 1   MTPSPAETIGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA 60
           M+PSPAE IG+SGTGDSQRSIPTPFLTKT+QLVDDPAVDDLISWNEDGSTFIVWRPAEFA
Sbjct: 1   MSPSPAEPIGESGTGDSQRSIPTPFLTKTFQLVDDPAVDDLISWNEDGSTFIVWRPAEFA 60

Query: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVALSV 120
           RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVALSV
Sbjct: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVALSV 120

Query: 121 TTTSPASPAAVAVPVTVVASPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPEL 180
            TT P +PAA+  PVTV A+PAV AHVISPANS EEQVTSSNSSPMAFQR TSCTTTPEL
Sbjct: 121 ATT-PPTPAAMVSPVTVAAAPAV-AHVISPANSGEEQVTSSNSSPMAFQRGTSCTTTPEL 180

Query: 181 VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHHHQLESVSVRDGKALELLPAR 240
           +RENERLRKENMQLSHELTQLKGLCNNILSLMTNYASG  HQ ESVSVRDGKALEL+PA 
Sbjct: 181 LRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASG--HQSESVSVRDGKALELMPAT 240

Query: 241 QV-MEDEGAVSDGAHEVRLKMEETMTAAAAAV-GMTPKLFGVSIGVKRMRRDEEDEEEEM 300
           QV MEDEGAVSDG  E+RLK+EE  TAAAAA  G+TPKLFGVSIGVKR+RR+EE+EEEEM
Sbjct: 241 QVMMEDEGAVSDGIQELRLKVEEATTAAAAAAEGVTPKLFGVSIGVKRVRREEEEEEEEM 300

Query: 301 VGQNHVQSEEGETGSEIKAEPLDENSENPEGSASPWLELGNQGS 343
           VGQNHVQSEEGE GSEIKAEPLDENS+NPEGSAS WLELGNQGS
Sbjct: 301 VGQNHVQSEEGENGSEIKAEPLDENSDNPEGSASQWLELGNQGS 340

BLAST of HG10009174 vs. ExPASy TrEMBL
Match: A0A6J1H317 (heat stress transcription factor B-2b-like OS=Cucurbita moschata OX=3662 GN=LOC111459647 PE=3 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 5.5e-155
Identity = 300/343 (87.46%), Postives = 313/343 (91.25%), Query Frame = 0

Query: 1   MTPSPAETIGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA 60
           M PSPAE IGDSGTGDSQRSIPTPFLTKTYQLVDDP VDDLISWNEDGSTFIVWRPAEFA
Sbjct: 1   MAPSPAEPIGDSGTGDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFA 60

Query: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVALSV 120
           RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCF+RGEK LLRDIQRRKVALSV
Sbjct: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVALSV 120

Query: 121 TTTSPASPAAVAVP-VTVVASPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPE 180
              +PA+PA+V+VP VTV ASPAV A VISP NSAEEQVTSSNSSPM FQR TSC TTPE
Sbjct: 121 --AAPATPASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPMEFQRGTSCCTTPE 180

Query: 181 LVRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHHHQLESVSVRDGKALELLPA 240
           LVRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASG H QLESVSVRDGKAL+LLPA
Sbjct: 181 LVRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASG-HQQLESVSVRDGKALDLLPA 240

Query: 241 RQVMEDEGAVSDGAHEVRLKMEETMTAAAAAVGMTPKLFGVSIGVKRMRRDEEDEEEEMV 300
           RQ+M+DEGAVSDG  EVRLK+EE + A A A G TPKLFGVSIGVKR+RR+E+D  EEMV
Sbjct: 241 RQMMDDEGAVSDGIQEVRLKVEEAV-AVATAEGRTPKLFGVSIGVKRVRREEDD--EEMV 300

Query: 301 GQNHVQSEEGETGSEIKAEPLDENSENPEGSASPWLELGNQGS 343
           G NHVQSEE ETGSEIKAEPLDENSENPEGSAS WLELGNQGS
Sbjct: 301 GPNHVQSEECETGSEIKAEPLDENSENPEGSASQWLELGNQGS 337

BLAST of HG10009174 vs. TAIR 10
Match: AT4G11660.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 350.5 bits (898), Expect = 1.5e-96
Identity = 203/346 (58.67%), Postives = 243/346 (70.23%), Query Frame = 0

Query: 10  GDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFARDLLPKYFK 69
           G  G GDSQRSIPTPFLTKTYQLV+DP  D+LISWNEDG+TFIVWRPAEFARDLLPKYFK
Sbjct: 45  GGGGGGDSQRSIPTPFLTKTYQLVEDPVYDELISWNEDGTTFIVWRPAEFARDLLPKYFK 104

Query: 70  HNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVAL-SVTTTSPASP 129
           HNNFSSFVRQLNTYGFRKVVPDRWEF+NDCF+RGEK LLRDIQRRK++  ++   + A+ 
Sbjct: 105 HNNFSSFVRQLNTYGFRKVVPDRWEFSNDCFKRGEKILLRDIQRRKISQPAMAAAAAAAA 164

Query: 130 AAVAVPVTVVASPAVLAHVISPANSAEEQVTSSNSSPMA-------------FQRSTSCT 189
           AAVA     VA+  V+AH++SP+NS EEQV SSNSSP A              QR+TSCT
Sbjct: 165 AAVAASAVTVAAVPVVAHIVSPSNSGEEQVISSNSSPAAAAAAIGGVVGGGSLQRTTSCT 224

Query: 190 TTPELVRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHHHQLESVSVRDGKALE 249
           T PELV ENERLRK+N +L  E+T+LKGL  NI +LM N+  G       +   +GK L+
Sbjct: 225 TAPELVEENERLRKDNERLRKEMTKLKGLYANIYTLMANFTPGQEDCAHLLP--EGKPLD 284

Query: 250 LLPARQVMEDEGAVSDGAHEVRLKMEETMTAAAAAVGMTPKLFGVSIGVKRMRRDEE--D 309
           LLP RQ M +    S+    + LK+ E          +TP+LFGVSIGVKR RR+EE   
Sbjct: 285 LLPERQEMSEAIMASEIETGIGLKLGE---------DLTPRLFGVSIGVKRARREEELGA 344

Query: 310 EEEEMVGQNHVQSEEGETGSEIKAEPLDE-NSENPEGSASPWLELG 339
            EEE   +    ++EGE  S++KAEP++E NS N  GS   WLELG
Sbjct: 345 AEEEDDDRREAAAQEGEQSSDVKAEPMEENNSGNHNGS---WLELG 376

BLAST of HG10009174 vs. TAIR 10
Match: AT5G62020.1 (heat shock transcription factor B2A )

HSP 1 Score: 234.6 bits (597), Expect = 1.2e-61
Identity = 143/300 (47.67%), Postives = 195/300 (65.00%), Query Frame = 0

Query: 12  SGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFARDLLPKYFKHN 71
           +G   SQRSIPTPFLTKT+ LV+D ++DD+ISWNEDGS+FIVW P +FA+DLLPK+FKHN
Sbjct: 11  TGESSSQRSIPTPFLTKTFNLVEDSSIDDVISWNEDGSSFIVWNPTDFAKDLLPKHFKHN 70

Query: 72  NFSSFVRQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVALSVTTTSPASPAAV 131
           NFSSFVRQLNTYGF+KVVPDRWEF+ND F+RGEK LLR+IQRRK    +TTT       V
Sbjct: 71  NFSSFVRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRK----ITTTH----QTV 130

Query: 132 AVPVTVVASPAVLAHVISPANSAEEQVTSS--NSSPMAFQRSTSCTT-----TPELVREN 191
             P +   +  +   V+SP+NS E+   +   +SSP ++    + TT     + EL+ EN
Sbjct: 131 VAPSSEQRNQTM---VVSPSNSGEDNNNNQVMSSSPSSWYCHQTKTTGNGGLSVELLEEN 190

Query: 192 ERLRKENMQLSHELTQLKGLCNNILSLMTNY-ASGHHHQLESVSVRDGKALELLPARQVM 251
           E+LR +N+QL+ ELTQ+K +C+NI SLM+NY  S    +  S      + +E LPA++  
Sbjct: 191 EKLRSQNIQLNRELTQMKSICDNIYSLMSNYVGSQPTDRSYSPGGSSSQPMEFLPAKRFS 250

Query: 252 EDEGAVSDGAHEVRLKMEETMTAAAAAVGMTPKLFGVSIGVKRMRRD-EEDEEEEMVGQN 303
           E             +++EE   A       +P+LFGV IG+KR R +  + +   +VG+N
Sbjct: 251 E-------------MEIEEEEEA-------SPRLFGVPIGLKRTRSEGVQVKTTAVVGEN 279

BLAST of HG10009174 vs. TAIR 10
Match: AT4G36990.1 (heat shock factor 4 )

HSP 1 Score: 192.6 bits (488), Expect = 5.3e-49
Identity = 119/285 (41.75%), Postives = 166/285 (58.25%), Query Frame = 0

Query: 17  SQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFARDLLPKYFKHNNFSSF 76
           +QRS+P PFL+KTYQLVDD + DD++SWNE+G+ F+VW+ AEFA+DLLP+YFKHNNFSSF
Sbjct: 7   AQRSVPAPFLSKTYQLVDDHSTDDVVSWNEEGTAFVVWKTAEFAKDLLPQYFKHNNFSSF 66

Query: 77  VRQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVALSVTTTSPASPAAVAVPVT 136
           +RQLNTYGFRK VPD+WEFAND FRRG + LL DI+RRK  +          A+ A    
Sbjct: 67  IRQLNTYGFRKTVPDKWEFANDYFRRGGEDLLTDIRRRKSVI----------ASTAGKCV 126

Query: 137 VVASPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSC-TTTPELVRENERLRKENMQLS 196
           VV SP+      S +   ++  +SS SSP + +   S      +L  ENE+L++EN  LS
Sbjct: 127 VVGSPSE-----SNSGGGDDHGSSSTSSPGSSKNPGSVENMVADLSGENEKLKRENNNLS 186

Query: 197 HELTQLKGLCNNILSLMTNYASGHHHQLESVSVRDGKALELLPARQVMEDEGAVSDGAHE 256
            EL   K   + +++ +T +      Q++ + ++ GK     P     E E    DG   
Sbjct: 187 SELAAAKKQRDELVTFLTGHLKVRPEQIDKM-IKGGK---FKPVESDEESECEGCDGG-- 246

Query: 257 VRLKMEETMTAAAAAVGMTPKLFGVSIGVKRMRRDEEDEEEEMVG 301
                      A   VG   KLFGV +  +R +RD +++   + G
Sbjct: 247 ---------GGAEEGVGEGLKLFGVWLKGERKKRDRDEKNYVVSG 261

BLAST of HG10009174 vs. TAIR 10
Match: AT1G46264.1 (heat shock transcription factor B4 )

HSP 1 Score: 176.4 bits (446), Expect = 3.9e-44
Identity = 98/203 (48.28%), Postives = 124/203 (61.08%), Query Frame = 0

Query: 19  RSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFARDLLPKYFKHNNFSSFVR 78
           +++P PFLTKTYQLVDDPA D ++SW +D +TF+VWRP EFARDLLP YFKHNNFSSFVR
Sbjct: 29  KAVPAPFLTKTYQLVDDPATDHVVSWGDDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR 88

Query: 79  QLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVALSVTTTSPASPAAVAVPVTVV 138
           QLNTYGFRK+VPDRWEFAN+ F+RGEK LL +I RRK +  +        +    P  + 
Sbjct: 89  QLNTYGFRKIVPDRWEFANEFFKRGEKHLLCEIHRRKTSQMIPQQHSPFMSHHHAPPQIP 148

Query: 139 AS-----PAVLAHVISPANSAEEQVTSSNSSPMAF-QRSTSCTTTPELVRENERLRKENM 198
            S     P     V +P         S  S P    Q+  +      L  +NERLR+ N 
Sbjct: 149 FSGGSFFPLPPPRVTTPEEDHYWCDDSPPSRPRVIPQQIDTAAQVTALSEDNERLRRSNT 208

Query: 199 QLSHELTQLKGLCNNILSLMTNY 216
            L  EL  +K L N+I+  + N+
Sbjct: 209 VLMSELAHMKKLYNDIIYFVQNH 231

BLAST of HG10009174 vs. TAIR 10
Match: AT2G41690.1 (heat shock transcription factor B3 )

HSP 1 Score: 150.6 bits (379), Expect = 2.3e-36
Identity = 93/232 (40.09%), Postives = 130/232 (56.03%), Query Frame = 0

Query: 5   PAETIGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFARDLL 64
           P E +  + T  ++   P PFL KTY++V+DP  D +ISWNE G+ F+VW+PAEFARDLL
Sbjct: 21  PLEFMIGNSTSTAELQPPPPFLVKTYKVVEDPTTDGVISWNEYGTGFVVWQPAEFARDLL 80

Query: 65  PKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVALSVTTTS 124
           P  FKH NFSSFVRQLNTYGFRKV   RWEF+N+ FR+G++ L+ +I+RRK   S   + 
Sbjct: 81  PTLFKHCNFSSFVRQLNTYGFRKVTTIRWEFSNEMFRKGQRELMSNIRRRK---SQHWSH 140

Query: 125 PASPAAVAVPVTVVASPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPELVREN 184
             S   V    T+V         I   +  E+Q +S+ SS   +           L+ EN
Sbjct: 141 NKSNHQVVPTTTMVNQEG--HQRIGIDHHHEDQQSSATSSSFVY---------TALLDEN 200

Query: 185 ERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHHHQLESVSVRDGKALEL 237
           + L+ EN  LS EL + K  C  ++ L+  Y        +     + + L+L
Sbjct: 201 KCLKNENELLSCELGKTKKKCKQLMELVERYRGEDEDATDESDDEEDEGLKL 238

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004143930.11.2e-17294.15heat stress transcription factor B-2b [Cucumis sativus] >XP_031741133.1 heat str... [more]
KAE8647868.11.2e-17294.15hypothetical protein Csa_000645 [Cucumis sativus][more]
XP_038875590.11.6e-17294.17heat stress transcription factor B-2b [Benincasa hispida] >XP_038875591.1 heat s... [more]
KAA0042789.11.5e-17093.27bifunctional UDP-glucose 4-epimerase and UDP-xylose 4-epimerase 1 [Cucumis melo ... [more]
XP_008437221.16.9e-16892.69PREDICTED: heat stress transcription factor B-2b [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q9T0D32.1e-9558.67Heat stress transcription factor B-2b OS=Arabidopsis thaliana OX=3702 GN=HSFB2B ... [more]
Q6Z9C84.5e-6949.25Heat stress transcription factor B-2b OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q652B05.8e-6949.86Heat stress transcription factor B-2c OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q9SCW41.7e-6047.67Heat stress transcription factor B-2a OS=Arabidopsis thaliana OX=3702 GN=HSFB2A ... [more]
P223354.6e-5041.36Heat shock factor protein HSF24 OS=Solanum peruvianum OX=4082 GN=HSF24 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KNZ85.9e-17394.15HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G155480 ... [more]
A0A5A7TN467.2e-17193.27Bifunctional UDP-glucose 4-epimerase and UDP-xylose 4-epimerase 1 OS=Cucumis mel... [more]
A0A1S3AU163.3e-16892.69heat stress transcription factor B-2b OS=Cucumis melo OX=3656 GN=LOC103482716 PE... [more]
A0A6J1DU117.4e-16088.95heat stress transcription factor B-2b OS=Momordica charantia OX=3673 GN=LOC11102... [more]
A0A6J1H3175.5e-15587.46heat stress transcription factor B-2b-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT4G11660.11.5e-9658.67winged-helix DNA-binding transcription factor family protein [more]
AT5G62020.11.2e-6147.67heat shock transcription factor B2A [more]
AT4G36990.15.3e-4941.75heat shock factor 4 [more]
AT1G46264.13.9e-4448.28heat shock transcription factor B4 [more]
AT2G41690.12.3e-3640.09heat shock transcription factor B3 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 184..204
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 325..342
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 155..176
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 288..323
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 288..342
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availablePANTHERPTHR10015:SF169HEAT STRESS TRANSCRIPTION FACTOR B-2Bcoord: 11..329
NoneNo IPR availableCDDcd14686bZIPcoord: 179..202
e-value: 0.00729268
score: 32.5173
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 25..48
score: 48.69
coord: 63..75
score: 81.98
coord: 76..88
score: 87.03
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 21..114
e-value: 7.4E-59
score: 211.5
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 25..114
e-value: 3.2E-32
score: 111.0
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 64..88
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 17..115
e-value: 1.1E-37
score: 130.6
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 11..329
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 22..114

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10009174.1HG10009174.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006012 galactose metabolic process
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function GO:0003978 UDP-glucose 4-epimerase activity
molecular_function GO:0043565 sequence-specific DNA binding