HG10009120 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10009120
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionubiquitin-conjugating enzyme E2 4-like
LocationChr06: 2696976 .. 2698813 (+)
RNA-Seq ExpressionHG10009120
SyntenyHG10009120
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTCTCCAAGCAAGCGACGTGAAATGGATTTGATGAAACTGTAAGTTTTTTTTTCAATTTCTTATGCCTTCCGATTCGATTGTTTCATCCTTCTTCTTCTTGGGTTGTTGTCTTTTTTTCCCCTTTACCCCGTTCTTCTTTGACTGAGCTGATTTTGAATCAGGATGATGAGTGATTACAAGGTGGAAACAATCAATGATAGCATGCAAGAGTTCTATGTGGACTTCCATGGACCTAAAGACAGTAAATCATCTATACCCATCTTTGATTTTTGCCCTTTTTGTGATTTATTGGTTGTTTTTCTTCTGAATTTAGGATTAACTCTTCATCTTTCACTTGGGTTTTGTTGTTAATTTTGTTGATTCTATCTCTTTAGGATTTCCCTCTTTTTCTTCAGATGGATGAGAACTTTCTAGCCATATAATTCATTGTATTGGGACATGTTATGATACTTTGCCTTTGTTTGAATCAACCTGAAGTTTGGGTTTTAATTTTCTGAGCTTCTTGTGTGGTTGACTAGTGTCAAATTGTAGTTTTGTTCTTCTTAATCTAGAGTTGTAATTATTGCTGACTTTCCCATTTGAAATTAAGCCAATTGATGTATACATTTCTAGGCAATTTGAATTTGGATTAAAGGATTTTTTTGTATCTTATCCCTTTTCCCTTCTTTGTTTGTTTTTGTTGAAATTGGAAGGTATTTATCAAGGTGGTGTGTGGAGGATTAGGGTGGAGTTGCCAGATGCTTATCCTTACAAGTCACCTTCAATTGGCTTTGTCAACAAAATTTATCATCCAAATGTTGATGAAATGTGAGTTGCTCAATTTCGACATCGAAACTTATTACATTGGTTCTTAATATGGATAGGACCTCATCTTCTTATGGGGTAGATTGTTGAGATTGATATACATGTTAATGAATTGCAGGTCGGGCTCAGTCTGTTTGGATGTTATCAACCAGACATGGAGCCCAATGTTTGGTAGGAAACTTCTTTGTGGAATTATATTGTTATCAGTTCTGTTGTACATATTATGGTGATCAGTTGGTTTATGCTTACTGGAACTATAATGATGCAACTGAGGAATTGTGATCTAATTCGGGTATCTTTCGTTGTGCAGACCTTGTAAATGTTTTCGAAGTGTTTCTTCCACAACTTCTCCTGTATCCCAACCCTTCGGATCCGTTGAATGGAGAAGCTGCTGCAGTAATGATGCGTGATCGCACTGCATATGAGCAAAGAGTAAAAGGTACTTCTATTGAAGATTTGGTAGTTTGAGTGAATTTTAAACTGTTGATTCGATCGGTAGTATAAAATTTTCTACATGATAATGGCAGATAGAATTTTCTTATTGCAAACTTAATTCTTGGCTGTTGATGTTGTGCTAATTCACTTGATAGTCTTACCAGCCCTGAGCTTCATCTGTAAAGAGATTCATAGTTTAGTGATGATGGCTCACCATTGTGCTAACCCCTTTTGATGTTCTGCATCTTTGAATCTGTGATGATGATTCTGAAATTACTGATAATGGTCCAGATGCATCGACATTTTTTGATCTCTCTCATACATTTAACGGTTATAAACTTAGCAGCCACATCACACCCCCACCCAACACACAGTGTTCAAAACTCTACATGGTTCGAGTCCTGTTAGAACCCTCTATCTCGGCCGTCTTTTTCTTTTACATTTCATTTGTGTTATGTACAGAATACTGTGAGAAATATGCAAAGCCAGAGGATATTGGTGCTGTTGCTGAGGAGAAATCTAGTGATGAGGAGCTGAGTGAAGATGATTATTCCTCAAGTGATGAGGCCATGGCAGGCCAAGCTGATGCTTGA

mRNA sequence

ATGTCTTCTCCAAGCAAGCGACGTGAAATGGATTTGATGAAACTGATGATGAGTGATTACAAGGTGGAAACAATCAATGATAGCATGCAAGAGTTCTATGTGGACTTCCATGGACCTAAAGACAGTATTTATCAAGGTGGTGTGTGGAGGATTAGGGTGGAGTTGCCAGATGCTTATCCTTACAAGTCACCTTCAATTGGCTTTGTCAACAAAATTTATCATCCAAATGTTGATGAAATGTCGGGCTCAGTCTGTTTGGATGTTATCAACCAGACATGGAGCCCAATGTTTGACCTTGTAAATGTTTTCGAAGTGTTTCTTCCACAACTTCTCCTGTATCCCAACCCTTCGGATCCGTTGAATGGAGAAGCTGCTGCAGTAATGATGCGTGATCGCACTGCATATGAGCAAAGAGTAAAAGAATACTGTGAGAAATATGCAAAGCCAGAGGATATTGGTGCTGTTGCTGAGGAGAAATCTAGTGATGAGGAGCTGAGTGAAGATGATTATTCCTCAAGTGATGAGGCCATGGCAGGCCAAGCTGATGCTTGA

Coding sequence (CDS)

ATGTCTTCTCCAAGCAAGCGACGTGAAATGGATTTGATGAAACTGATGATGAGTGATTACAAGGTGGAAACAATCAATGATAGCATGCAAGAGTTCTATGTGGACTTCCATGGACCTAAAGACAGTATTTATCAAGGTGGTGTGTGGAGGATTAGGGTGGAGTTGCCAGATGCTTATCCTTACAAGTCACCTTCAATTGGCTTTGTCAACAAAATTTATCATCCAAATGTTGATGAAATGTCGGGCTCAGTCTGTTTGGATGTTATCAACCAGACATGGAGCCCAATGTTTGACCTTGTAAATGTTTTCGAAGTGTTTCTTCCACAACTTCTCCTGTATCCCAACCCTTCGGATCCGTTGAATGGAGAAGCTGCTGCAGTAATGATGCGTGATCGCACTGCATATGAGCAAAGAGTAAAAGAATACTGTGAGAAATATGCAAAGCCAGAGGATATTGGTGCTGTTGCTGAGGAGAAATCTAGTGATGAGGAGCTGAGTGAAGATGATTATTCCTCAAGTGATGAGGCCATGGCAGGCCAAGCTGATGCTTGA

Protein sequence

MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSIYQGGVWRIRVELPDAYPYKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPLNGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVAEEKSSDEELSEDDYSSSDEAMAGQADA
Homology
BLAST of HG10009120 vs. NCBI nr
Match: XP_004143895.1 (ubiquitin-conjugating enzyme E2 4 [Cucumis sativus] >XP_008437307.1 PREDICTED: ubiquitin-conjugating enzyme E2 4-like [Cucumis melo] >XP_038906796.1 ubiquitin-conjugating enzyme E2 4-like [Benincasa hispida] >KAA0042743.1 ubiquitin-conjugating enzyme E2 4-like isoform X1 [Cucumis melo var. makuwa] >KGN50082.1 hypothetical protein Csa_000238 [Cucumis sativus])

HSP 1 Score: 363.2 bits (931), Expect = 1.3e-96
Identity = 180/183 (98.36%), Postives = 181/183 (98.91%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSIYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDS+YQGGVWRIRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL
Sbjct: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVAEEKSSDEELSEDDYSSSDEAMAGQ 180
           NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAV EEKSSDEELSEDDYSS DEAMAGQ
Sbjct: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQ 180

Query: 181 ADA 184
           ADA
Sbjct: 181 ADA 183

BLAST of HG10009120 vs. NCBI nr
Match: XP_022159827.1 (ubiquitin-conjugating enzyme E2 4-like [Momordica charantia])

HSP 1 Score: 359.0 bits (920), Expect = 2.4e-95
Identity = 178/183 (97.27%), Postives = 180/183 (98.36%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSIYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKVE INDSMQEFYVDFHGPKDS+YQGGVWRIRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVEMINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL
Sbjct: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVAEEKSSDEELSEDDYSSSDEAMAGQ 180
           NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAV EEKSSDEELSEDDY+SSDE MAGQ
Sbjct: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYASSDEDMAGQ 180

Query: 181 ADA 184
           ADA
Sbjct: 181 ADA 183

BLAST of HG10009120 vs. NCBI nr
Match: XP_022996146.1 (ubiquitin-conjugating enzyme E2 4-like [Cucurbita maxima])

HSP 1 Score: 357.1 bits (915), Expect = 9.3e-95
Identity = 175/183 (95.63%), Postives = 179/183 (97.81%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSIYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDS+YQGGVWRIRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPSIGF+NKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL
Sbjct: 61  YKSPSIGFINKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVAEEKSSDEELSEDDYSSSDEAMAGQ 180
           NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIG V EEKSSDEE+SED+YSS DE MAGQ
Sbjct: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGGVGEEKSSDEEVSEDEYSSDDEEMAGQ 180

Query: 181 ADA 184
           ADA
Sbjct: 181 ADA 183

BLAST of HG10009120 vs. NCBI nr
Match: XP_022958690.1 (ubiquitin-conjugating enzyme E2 4-like [Cucurbita moschata] >XP_023534777.1 ubiquitin-conjugating enzyme E2 4-like [Cucurbita pepo subsp. pepo] >KAG6606082.1 Ubiquitin-conjugating enzyme E2 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 355.1 bits (910), Expect = 3.5e-94
Identity = 174/183 (95.08%), Postives = 178/183 (97.27%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSIYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDS+YQGGVWRIRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPSIGF+NKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL
Sbjct: 61  YKSPSIGFINKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVAEEKSSDEELSEDDYSSSDEAMAGQ 180
           NGEAAAVMMRDR AYEQRVKEYCEKYAKPEDIG V EEKSSDEE+SED+YSS DE MAGQ
Sbjct: 121 NGEAAAVMMRDRAAYEQRVKEYCEKYAKPEDIGGVGEEKSSDEEVSEDEYSSDDEEMAGQ 180

Query: 181 ADA 184
           ADA
Sbjct: 181 ADA 183

BLAST of HG10009120 vs. NCBI nr
Match: KAG7036029.1 (Ubiquitin-conjugating enzyme E2 5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 355.1 bits (910), Expect = 3.5e-94
Identity = 174/183 (95.08%), Postives = 178/183 (97.27%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSIYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDS+YQGGVWRIRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPSIGF+NKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL
Sbjct: 61  YKSPSIGFINKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVAEEKSSDEELSEDDYSSSDEAMAGQ 180
           NGEAAAVMMRDR AYEQRVKEYCEKYAKPEDIG V EEKSSDEE+SED+YSS DE MAGQ
Sbjct: 121 NGEAAAVMMRDRAAYEQRVKEYCEKYAKPEDIGGVGEEKSSDEEVSEDEYSSEDEEMAGQ 180

Query: 181 ADA 184
           ADA
Sbjct: 181 ADA 183

BLAST of HG10009120 vs. ExPASy Swiss-Prot
Match: P42748 (Ubiquitin-conjugating enzyme E2 4 OS=Arabidopsis thaliana OX=3702 GN=UBC4 PE=2 SV=2)

HSP 1 Score: 310.8 bits (795), Expect = 1.0e-83
Identity = 154/189 (81.48%), Postives = 170/189 (89.95%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSIYQGGVWRIRVELPDAYP 60
           MSSPSKRREMD+MKLMMSDYKVETIND MQEFYV+F+GPKDS+YQGGVW+IRVELPDAYP
Sbjct: 1   MSSPSKRREMDMMKLMMSDYKVETINDGMQEFYVEFNGPKDSLYQGGVWKIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPS+GF+ KIYHPNVDE+SGSVCLDVINQTWSPMFDLVNVFE FLPQLLLYPNPSDPL
Sbjct: 61  YKSPSVGFITKIYHPNVDELSGSVCLDVINQTWSPMFDLVNVFETFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVAEEKSSDEELSEDDYSSSDE----- 180
           NGEAAA+MMRDR AYEQRVKEYCEKYAKP   G  +E+KSSDEELSE++Y S +E     
Sbjct: 121 NGEAAALMMRDRPAYEQRVKEYCEKYAKP---GEGSEDKSSDEELSEEEYGSDNEDDDDD 180

Query: 181 --AMAGQAD 183
             A+AG+ D
Sbjct: 181 DVAIAGKPD 186

BLAST of HG10009120 vs. ExPASy Swiss-Prot
Match: P16577 (Ubiquitin-conjugating enzyme E2-23 kDa OS=Triticum aestivum OX=4565 GN=UBC4 PE=1 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 1.4e-82
Identity = 149/183 (81.42%), Postives = 164/183 (89.62%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSIYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKV+ IND M EF+V FHGPKDSIYQGGVW++RVEL +AYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVDMINDGMHEFFVHFHGPKDSIYQGGVWKVRVELTEAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPSIGF NKIYHPNVDEMSGSVCLDVINQTWSPMFDLVN+FEVFLPQLLLYPNPSDPL
Sbjct: 61  YKSPSIGFTNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNIFEVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVAEEKSSDEELSE-DDYSSSDEAMAG 180
           NGEAA++MMRD+ AYE +VKEYCE+YAKPEDI    EE+ SDEELS+ + Y S DEA+ G
Sbjct: 121 NGEAASLMMRDKNAYENKVKEYCERYAKPEDISPEEEEEESDEELSDAEGYDSGDEAIMG 180

Query: 181 QAD 183
            AD
Sbjct: 181 HAD 183

BLAST of HG10009120 vs. ExPASy Swiss-Prot
Match: P42749 (Ubiquitin-conjugating enzyme E2 5 OS=Arabidopsis thaliana OX=3702 GN=UBC5 PE=2 SV=2)

HSP 1 Score: 303.5 bits (776), Expect = 1.6e-81
Identity = 152/187 (81.28%), Postives = 165/187 (88.24%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSIYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKVE IND MQEF+V+F GPKDSIY+GGVW+IRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVEMINDGMQEFFVEFSGPKDSIYEGGVWKIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPS+GF+ KIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFE FLPQLLLYPNPSDPL
Sbjct: 61  YKSPSVGFITKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFETFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVAEEKSSDEELSEDDYSS-----SDE 180
           NGEAAA+MMRDR  YEQRVKEYCEKYAKP    A  EE SSD+E+SED+Y+S      D 
Sbjct: 121 NGEAAALMMRDRPTYEQRVKEYCEKYAKPR---ADTEEMSSDDEMSEDEYASDGDDEDDV 180

Query: 181 AMAGQAD 183
           A+AG+ D
Sbjct: 181 AIAGKLD 184

BLAST of HG10009120 vs. ExPASy Swiss-Prot
Match: P42750 (Ubiquitin-conjugating enzyme E2 6 OS=Arabidopsis thaliana OX=3702 GN=UBC6 PE=2 SV=2)

HSP 1 Score: 282.0 bits (720), Expect = 5.0e-75
Identity = 133/182 (73.08%), Postives = 156/182 (85.71%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSIYQGGVWRIRVELPDAYP 60
           M+SPSKRREMD+MKLMMSDYKV+T+ND +Q FYV FHGP DS+YQGGVW+I+VELP+AYP
Sbjct: 1   MASPSKRREMDMMKLMMSDYKVDTVNDDLQMFYVTFHGPTDSLYQGGVWKIKVELPEAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPS+GFVNKIYHPNVDE SG+VCLDVINQTWSPMFDL+NVFE FLPQLLLYPNPSDP 
Sbjct: 61  YKSPSVGFVNKIYHPNVDESSGAVCLDVINQTWSPMFDLINVFESFLPQLLLYPNPSDPF 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVAEEKSSDEELSEDDYSSSDEAMAGQ 180
           NGEAA+++MRDR AYE +VKEYCEKYAKPE+I +  ++  S  E   D     D+ + G+
Sbjct: 121 NGEAASLLMRDRAAYELKVKEYCEKYAKPEEILSDDDDDDSMSEDGSDSDDDDDDEIVGK 180

Query: 181 AD 183
           AD
Sbjct: 181 AD 182

BLAST of HG10009120 vs. ExPASy Swiss-Prot
Match: Q9P7R4 (Ubiquitin-conjugating enzyme E2 8 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ubc8 PE=3 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 1.1e-61
Identity = 111/183 (60.66%), Postives = 143/183 (78.14%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSIYQGGVWRIRVELPDAYP 60
           MSSP +R E D+MKL+MSDY+V  +ND+MQEFYV FHGP ++ Y GG+W++ VELP  YP
Sbjct: 1   MSSPRRRIETDVMKLLMSDYEVTLVNDNMQEFYVRFHGPSETPYSGGIWKVHVELPSEYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           +KSPSIGFVN+I+HPN+DE+SGSVCLDVINQTWSPMFD++N+FEVFLPQLL YPN SDPL
Sbjct: 61  WKSPSIGFVNRIFHPNIDELSGSVCLDVINQTWSPMFDMINIFEVFLPQLLRYPNASDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVAEEKSSDEELSEDDYSSSDEAMAGQ 180
           NGEAAA+++R+   Y  +V++Y  +YA  ED      + S D+ +S  D  S DE +AG 
Sbjct: 121 NGEAAALLLREPNTYYAKVRDYIARYANKEDADITLNDSSDDDTMSSIDTESGDE-IAGP 180

Query: 181 ADA 184
            D+
Sbjct: 181 MDS 182

BLAST of HG10009120 vs. ExPASy TrEMBL
Match: A0A5A7THI1 (Ubiquitin-conjugating enzyme E2 4-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002420 PE=3 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 6.3e-97
Identity = 180/183 (98.36%), Postives = 181/183 (98.91%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSIYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDS+YQGGVWRIRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL
Sbjct: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVAEEKSSDEELSEDDYSSSDEAMAGQ 180
           NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAV EEKSSDEELSEDDYSS DEAMAGQ
Sbjct: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQ 180

Query: 181 ADA 184
           ADA
Sbjct: 181 ADA 183

BLAST of HG10009120 vs. ExPASy TrEMBL
Match: A0A0A0KNV9 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G152970 PE=3 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 6.3e-97
Identity = 180/183 (98.36%), Postives = 181/183 (98.91%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSIYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDS+YQGGVWRIRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL
Sbjct: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVAEEKSSDEELSEDDYSSSDEAMAGQ 180
           NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAV EEKSSDEELSEDDYSS DEAMAGQ
Sbjct: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQ 180

Query: 181 ADA 184
           ADA
Sbjct: 181 ADA 183

BLAST of HG10009120 vs. ExPASy TrEMBL
Match: A0A1S3AUA7 (ubiquitin-conjugating enzyme E2 4-like OS=Cucumis melo OX=3656 GN=LOC103482765 PE=3 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 6.3e-97
Identity = 180/183 (98.36%), Postives = 181/183 (98.91%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSIYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDS+YQGGVWRIRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL
Sbjct: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVAEEKSSDEELSEDDYSSSDEAMAGQ 180
           NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAV EEKSSDEELSEDDYSS DEAMAGQ
Sbjct: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYSSGDEAMAGQ 180

Query: 181 ADA 184
           ADA
Sbjct: 181 ADA 183

BLAST of HG10009120 vs. ExPASy TrEMBL
Match: A0A6J1E0W4 (ubiquitin-conjugating enzyme E2 4-like OS=Momordica charantia OX=3673 GN=LOC111026131 PE=3 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 1.2e-95
Identity = 178/183 (97.27%), Postives = 180/183 (98.36%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSIYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKVE INDSMQEFYVDFHGPKDS+YQGGVWRIRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVEMINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL
Sbjct: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVAEEKSSDEELSEDDYSSSDEAMAGQ 180
           NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAV EEKSSDEELSEDDY+SSDE MAGQ
Sbjct: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVPEEKSSDEELSEDDYASSDEDMAGQ 180

Query: 181 ADA 184
           ADA
Sbjct: 181 ADA 183

BLAST of HG10009120 vs. ExPASy TrEMBL
Match: A0A6J1K3W2 (ubiquitin-conjugating enzyme E2 4-like OS=Cucurbita maxima OX=3661 GN=LOC111491458 PE=3 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 4.5e-95
Identity = 175/183 (95.63%), Postives = 179/183 (97.81%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSIYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDS+YQGGVWRIRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSLYQGGVWRIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPSIGF+NKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL
Sbjct: 61  YKSPSIGFINKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVAEEKSSDEELSEDDYSSSDEAMAGQ 180
           NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIG V EEKSSDEE+SED+YSS DE MAGQ
Sbjct: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGGVGEEKSSDEEVSEDEYSSDDEEMAGQ 180

Query: 181 ADA 184
           ADA
Sbjct: 181 ADA 183

BLAST of HG10009120 vs. TAIR 10
Match: AT5G41340.1 (ubiquitin conjugating enzyme 4 )

HSP 1 Score: 310.8 bits (795), Expect = 7.1e-85
Identity = 154/189 (81.48%), Postives = 170/189 (89.95%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSIYQGGVWRIRVELPDAYP 60
           MSSPSKRREMD+MKLMMSDYKVETIND MQEFYV+F+GPKDS+YQGGVW+IRVELPDAYP
Sbjct: 1   MSSPSKRREMDMMKLMMSDYKVETINDGMQEFYVEFNGPKDSLYQGGVWKIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPS+GF+ KIYHPNVDE+SGSVCLDVINQTWSPMFDLVNVFE FLPQLLLYPNPSDPL
Sbjct: 61  YKSPSVGFITKIYHPNVDELSGSVCLDVINQTWSPMFDLVNVFETFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVAEEKSSDEELSEDDYSSSDE----- 180
           NGEAAA+MMRDR AYEQRVKEYCEKYAKP   G  +E+KSSDEELSE++Y S +E     
Sbjct: 121 NGEAAALMMRDRPAYEQRVKEYCEKYAKP---GEGSEDKSSDEELSEEEYGSDNEDDDDD 180

Query: 181 --AMAGQAD 183
             A+AG+ D
Sbjct: 181 DVAIAGKPD 186

BLAST of HG10009120 vs. TAIR 10
Match: AT1G63800.1 (ubiquitin-conjugating enzyme 5 )

HSP 1 Score: 303.5 bits (776), Expect = 1.1e-82
Identity = 152/187 (81.28%), Postives = 165/187 (88.24%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSIYQGGVWRIRVELPDAYP 60
           MSSPSKRREMDLMKLMMSDYKVE IND MQEF+V+F GPKDSIY+GGVW+IRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVEMINDGMQEFFVEFSGPKDSIYEGGVWKIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPS+GF+ KIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFE FLPQLLLYPNPSDPL
Sbjct: 61  YKSPSVGFITKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFETFLPQLLLYPNPSDPL 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVAEEKSSDEELSEDDYSS-----SDE 180
           NGEAAA+MMRDR  YEQRVKEYCEKYAKP    A  EE SSD+E+SED+Y+S      D 
Sbjct: 121 NGEAAALMMRDRPTYEQRVKEYCEKYAKPR---ADTEEMSSDDEMSEDEYASDGDDEDDV 180

Query: 181 AMAGQAD 183
           A+AG+ D
Sbjct: 181 AIAGKLD 184

BLAST of HG10009120 vs. TAIR 10
Match: AT2G46030.1 (ubiquitin-conjugating enzyme 6 )

HSP 1 Score: 282.0 bits (720), Expect = 3.5e-76
Identity = 133/182 (73.08%), Postives = 156/182 (85.71%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLMMSDYKVETINDSMQEFYVDFHGPKDSIYQGGVWRIRVELPDAYP 60
           M+SPSKRREMD+MKLMMSDYKV+T+ND +Q FYV FHGP DS+YQGGVW+I+VELP+AYP
Sbjct: 1   MASPSKRREMDMMKLMMSDYKVDTVNDDLQMFYVTFHGPTDSLYQGGVWKIKVELPEAYP 60

Query: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120
           YKSPS+GFVNKIYHPNVDE SG+VCLDVINQTWSPMFDL+NVFE FLPQLLLYPNPSDP 
Sbjct: 61  YKSPSVGFVNKIYHPNVDESSGAVCLDVINQTWSPMFDLINVFESFLPQLLLYPNPSDPF 120

Query: 121 NGEAAAVMMRDRTAYEQRVKEYCEKYAKPEDIGAVAEEKSSDEELSEDDYSSSDEAMAGQ 180
           NGEAA+++MRDR AYE +VKEYCEKYAKPE+I +  ++  S  E   D     D+ + G+
Sbjct: 121 NGEAASLLMRDRAAYELKVKEYCEKYAKPEEILSDDDDDDSMSEDGSDSDDDDDDEIVGK 180

Query: 181 AD 183
           AD
Sbjct: 181 AD 182

BLAST of HG10009120 vs. TAIR 10
Match: AT3G08690.1 (ubiquitin-conjugating enzyme 11 )

HSP 1 Score: 100.9 bits (250), Expect = 1.1e-21
Identity = 49/137 (35.77%), Postives = 75/137 (54.74%), Query Frame = 0

Query: 11  DLMKLMMSDYKVETINDSMQEFYVDFHGPKDSIYQGGVWRIRVELPDAYPYKSPSIGFVN 70
           DL K   S+     + + M  +     GP +S Y GGV+ + +  P  YP+K P + F  
Sbjct: 12  DLQKDPPSNCSAGPVAEDMFHWQATIMGPPESPYAGGVFLVSIHFPPDYPFKPPKVSFKT 71

Query: 71  KIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPLNGEAAAVMMR 130
           K+YHPN++  +GS+CLD++ + WSP   +  V  + +  LL  PNP DPL  E A +   
Sbjct: 72  KVYHPNINS-NGSICLDILKEQWSPALTISKVL-LSICSLLTDPNPDDPLVPEIAHMYKT 131

Query: 131 DRTAYEQRVKEYCEKYA 148
           DR+ YE   + + +KYA
Sbjct: 132 DRSKYESTARSWTQKYA 146

BLAST of HG10009120 vs. TAIR 10
Match: AT3G08690.2 (ubiquitin-conjugating enzyme 11 )

HSP 1 Score: 100.9 bits (250), Expect = 1.1e-21
Identity = 49/137 (35.77%), Postives = 75/137 (54.74%), Query Frame = 0

Query: 11  DLMKLMMSDYKVETINDSMQEFYVDFHGPKDSIYQGGVWRIRVELPDAYPYKSPSIGFVN 70
           DL K   S+     + + M  +     GP +S Y GGV+ + +  P  YP+K P + F  
Sbjct: 12  DLQKDPPSNCSAGPVAEDMFHWQATIMGPPESPYAGGVFLVSIHFPPDYPFKPPKVSFKT 71

Query: 71  KIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPLNGEAAAVMMR 130
           K+YHPN++  +GS+CLD++ + WSP   +  V  + +  LL  PNP DPL  E A +   
Sbjct: 72  KVYHPNINS-NGSICLDILKEQWSPALTISKVL-LSICSLLTDPNPDDPLVPEIAHMYKT 131

Query: 131 DRTAYEQRVKEYCEKYA 148
           DR+ YE   + + +KYA
Sbjct: 132 DRSKYESTARSWTQKYA 146

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004143895.11.3e-9698.36ubiquitin-conjugating enzyme E2 4 [Cucumis sativus] >XP_008437307.1 PREDICTED: u... [more]
XP_022159827.12.4e-9597.27ubiquitin-conjugating enzyme E2 4-like [Momordica charantia][more]
XP_022996146.19.3e-9595.63ubiquitin-conjugating enzyme E2 4-like [Cucurbita maxima][more]
XP_022958690.13.5e-9495.08ubiquitin-conjugating enzyme E2 4-like [Cucurbita moschata] >XP_023534777.1 ubiq... [more]
KAG7036029.13.5e-9495.08Ubiquitin-conjugating enzyme E2 5 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
P427481.0e-8381.48Ubiquitin-conjugating enzyme E2 4 OS=Arabidopsis thaliana OX=3702 GN=UBC4 PE=2 S... [more]
P165771.4e-8281.42Ubiquitin-conjugating enzyme E2-23 kDa OS=Triticum aestivum OX=4565 GN=UBC4 PE=1... [more]
P427491.6e-8181.28Ubiquitin-conjugating enzyme E2 5 OS=Arabidopsis thaliana OX=3702 GN=UBC5 PE=2 S... [more]
P427505.0e-7573.08Ubiquitin-conjugating enzyme E2 6 OS=Arabidopsis thaliana OX=3702 GN=UBC6 PE=2 S... [more]
Q9P7R41.1e-6160.66Ubiquitin-conjugating enzyme E2 8 OS=Schizosaccharomyces pombe (strain 972 / ATC... [more]
Match NameE-valueIdentityDescription
A0A5A7THI16.3e-9798.36Ubiquitin-conjugating enzyme E2 4-like isoform X1 OS=Cucumis melo var. makuwa OX... [more]
A0A0A0KNV96.3e-9798.36UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G152970 PE... [more]
A0A1S3AUA76.3e-9798.36ubiquitin-conjugating enzyme E2 4-like OS=Cucumis melo OX=3656 GN=LOC103482765 P... [more]
A0A6J1E0W41.2e-9597.27ubiquitin-conjugating enzyme E2 4-like OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1K3W24.5e-9595.63ubiquitin-conjugating enzyme E2 4-like OS=Cucurbita maxima OX=3661 GN=LOC1114914... [more]
Match NameE-valueIdentityDescription
AT5G41340.17.1e-8581.48ubiquitin conjugating enzyme 4 [more]
AT1G63800.11.1e-8281.28ubiquitin-conjugating enzyme 5 [more]
AT2G46030.13.5e-7673.08ubiquitin-conjugating enzyme 6 [more]
AT3G08690.11.1e-2135.77ubiquitin-conjugating enzyme 11 [more]
AT3G08690.21.1e-2135.77ubiquitin-conjugating enzyme 11 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 4..148
e-value: 3.0E-50
score: 182.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 153..183
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 3..182
NoneNo IPR availablePANTHERPTHR24068:SF321UBIQUITIN-CONJUGATING ENZYME E2-23 KDAcoord: 3..182
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 10..142
e-value: 4.9E-39
score: 133.2
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 1..148
score: 42.533482
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 7..143
e-value: 1.74919E-51
score: 160.06
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 1..160
e-value: 2.3E-50
score: 172.3
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 4..148
IPR023313Ubiquitin-conjugating enzyme, active sitePROSITEPS00183UBC_1coord: 73..89

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10009120.1HG10009120.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0061631 ubiquitin conjugating enzyme activity