HG10009059 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10009059 vs. NCBI nr
Match: XP_038876129.1 (probable polygalacturonase [Benincasa hispida])

HSP 1 Score: 887.9 bits (2293), Expect = 3.8e-254
Identity = 433/461 (93.93%), Postives = 439/461 (95.23%), Query Frame = 0

Query: 1   MVPFFLFIFIFFTTLFPSPLVIADLPTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60
           MVPFFLFIF +FTTLFPSPL +ADL TCSGIVPMKYRNDKISI DFGGIGDGRTLNTRAF
Sbjct: 1   MVPFFLFIFFYFTTLFPSPLAVADLTTCSGIVPMKYRNDKISITDFGGIGDGRTLNTRAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVFLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120
           RAAIYRIQHLRRRGGTLLYIPPGV+LTESFNLTSHMTLYLAKGAVI+AVQDSSNWPVIAP
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIRAVQDSSNWPVIAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV 180
           LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV 180

Query: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPALYILESN 240
           EFINSYNIIISNVMFLNSPFWNIHPVYC+NVVVRYVTILAPRDSPNTDGVDP        
Sbjct: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCTNVVVRYVTILAPRDSPNTDGVDP-------- 240

Query: 241 KPCKLMSTDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFA 300
                   DSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRR+SGSSPFA
Sbjct: 241 --------DSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFA 300

Query: 301 GVAVGSETSGGVENVLAEHLNFYDMGVGINIKTNIGRGGFIRNITVSNVYMESSRKGLKI 360
           GVAVGSETSGGVENVLAEHLNFYDMGVGINIKTNIGRGGFIRNITVSNVYMESSRKGLKI
Sbjct: 301 GVAVGSETSGGVENVLAEHLNFYDMGVGINIKTNIGRGGFIRNITVSNVYMESSRKGLKI 360

Query: 361 AGDTGDHPDDKYDPNALPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNINLRGA 420
           AGDTGDHPDDKYDPNALPIVKDITIKNVWG NVQQAGSIYGLRNSPFTGICLSNINLRGA
Sbjct: 361 AGDTGDHPDDKYDPNALPIVKDITIKNVWGENVQQAGSIYGLRNSPFTGICLSNINLRGA 420

Query: 421 TRPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDGLCSDNF 462
            RPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDGLCSDNF
Sbjct: 421 RRPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDGLCSDNF 445

BLAST of HG10009059 vs. NCBI nr
Match: XP_008437386.1 (PREDICTED: probable polygalacturonase [Cucumis melo] >KAA0042687.1 putative polygalacturonase [Cucumis melo var. makuwa] >TYK06090.1 putative polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 873.2 bits (2255), Expect = 9.7e-250
Identity = 425/461 (92.19%), Postives = 436/461 (94.58%), Query Frame = 0

Query: 1   MVPFFLFIFIFFTTLFPSPLVIADLPTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60
           MVPF LF+FIFFTTLFPSPL +ADL TCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF
Sbjct: 1   MVPFSLFVFIFFTTLFPSPLAVADLITCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVFLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120
           RAAIYRIQHLRRRGGTLLYIPPGV+LTE+FNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTETFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV 180
           LPSYGRGRERPGGRYVSFIHG GVHDVVITGENGTIDGQGDAWWNMWRTGTLK+TRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGHGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLV 180

Query: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPALYILESN 240
           EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDP        
Sbjct: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDP-------- 240

Query: 241 KPCKLMSTDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFA 300
                   DSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFA
Sbjct: 241 --------DSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFA 300

Query: 301 GVAVGSETSGGVENVLAEHLNFYDMGVGINIKTNIGRGGFIRNITVSNVYMESSRKGLKI 360
           GVAVGSETSGGV NVLAEHLNFYDMGVGINIKTNIGRGGFI+NITVSNVYME+SRKGLKI
Sbjct: 301 GVAVGSETSGGVANVLAEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKI 360

Query: 361 AGDTGDHPDDKYDPNALPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNINLRGA 420
           AGDTGDHPDDK+DPNALPIVKDITIKN WGV VQQAGSIYGLR+SPFTGICLSNIN RG 
Sbjct: 361 AGDTGDHPDDKFDPNALPIVKDITIKNAWGVKVQQAGSIYGLRDSPFTGICLSNINFRGT 420

Query: 421 TRPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDGLCSDNF 462
           TRPRSVPWTCSYVSGAAL+VSPWPCSELTST+QDGLCSDNF
Sbjct: 421 TRPRSVPWTCSYVSGAALMVSPWPCSELTSTEQDGLCSDNF 445

BLAST of HG10009059 vs. NCBI nr
Match: XP_004143871.1 (probable polygalacturonase [Cucumis sativus] >KGN50025.1 hypothetical protein Csa_000391 [Cucumis sativus])

HSP 1 Score: 866.7 bits (2238), Expect = 9.1e-248
Identity = 421/461 (91.32%), Postives = 434/461 (94.14%), Query Frame = 0

Query: 1   MVPFFLFIFIFFTTLFPSPLVIADLPTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60
           M+PF LFIFIFFTTLFPSPL +ADL TCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF
Sbjct: 1   MLPFPLFIFIFFTTLFPSPLAVADLTTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVFLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120
           RAAIYRIQHLRRRGGTLLYIPPGV+LTE+FNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTETFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV 180
           LPSYGRGRERPGGRY+S IHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLK+TRP+LV
Sbjct: 121 LPSYGRGRERPGGRYISLIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPSLV 180

Query: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPALYILESN 240
           EF+NSYNIIISNVMFLNSPFWNIHPVYC NVVVRYVTILAPRDSPNTDGVDP        
Sbjct: 181 EFVNSYNIIISNVMFLNSPFWNIHPVYCRNVVVRYVTILAPRDSPNTDGVDP-------- 240

Query: 241 KPCKLMSTDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFA 300
                   DSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGR SYDITIRRISGSSPFA
Sbjct: 241 --------DSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRCSYDITIRRISGSSPFA 300

Query: 301 GVAVGSETSGGVENVLAEHLNFYDMGVGINIKTNIGRGGFIRNITVSNVYMESSRKGLKI 360
           GVAVGSE SGGV NVLAEHLNFYDMGVGINIKTNIGRGGFI+NITVSNVYME+SRKGLKI
Sbjct: 301 GVAVGSEASGGVANVLAEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKI 360

Query: 361 AGDTGDHPDDKYDPNALPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNINLRGA 420
           AGD GDHPDDK+DPNALPIVKDITIKN+WGVNVQQAGSIYGLR+SPFTGICLSNINLRGA
Sbjct: 361 AGDAGDHPDDKFDPNALPIVKDITIKNIWGVNVQQAGSIYGLRDSPFTGICLSNINLRGA 420

Query: 421 TRPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDGLCSDNF 462
           TRPRSVPWTCSYVSGAA LVSPWPCSELTST QDGLCSDNF
Sbjct: 421 TRPRSVPWTCSYVSGAASLVSPWPCSELTSTDQDGLCSDNF 445

BLAST of HG10009059 vs. NCBI nr
Match: XP_022970040.1 (probable polygalacturonase [Cucurbita maxima] >XP_022970041.1 probable polygalacturonase [Cucurbita maxima])

HSP 1 Score: 851.7 bits (2199), Expect = 3.0e-243
Identity = 415/460 (90.22%), Postives = 428/460 (93.04%), Query Frame = 0

Query: 1   MVPFFLFIFIFFTTLFPSPLVIADLPTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60
           M+PF LFIFI+F TL PSP  IA L TCSGIVPM YRNDKISI DFGGIGDGRTLNT+AF
Sbjct: 1   MLPFSLFIFIYFATLLPSPFAIAQLTTCSGIVPMNYRNDKISITDFGGIGDGRTLNTKAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVFLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120
           RAAIYRIQ+L+RRGGTLLYIPPGV+LTESFNLTSHMTLYLAK AVIKAVQ SSNWPV+AP
Sbjct: 61  RAAIYRIQNLKRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQGSSNWPVVAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV 180
           LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV 180

Query: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPALYILESN 240
           EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDG+DP        
Sbjct: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDP-------- 240

Query: 241 KPCKLMSTDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFA 300
                   DSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRR+SGSSPFA
Sbjct: 241 --------DSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFA 300

Query: 301 GVAVGSETSGGVENVLAEHLNFYDMGVGINIKTNIGRGGFIRNITVSNVYMESSRKGLKI 360
           GVAVGSETSGGV NVLAEHLNFYDMG+GINIKTNIGRGGFI+NITVSNVYME SRKGLKI
Sbjct: 301 GVAVGSETSGGVANVLAEHLNFYDMGIGINIKTNIGRGGFIKNITVSNVYMERSRKGLKI 360

Query: 361 AGDTGDHPDDKYDPNALPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNINLRGA 420
           AGDTGDHPDDK+DPNALPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNI+LRGA
Sbjct: 361 AGDTGDHPDDKFDPNALPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNIHLRGA 420

Query: 421 TRPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDGLCSDN 461
            RPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDG CS N
Sbjct: 421 MRPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDGSCSAN 444

BLAST of HG10009059 vs. NCBI nr
Match: XP_023550399.1 (probable polygalacturonase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 850.1 bits (2195), Expect = 8.8e-243
Identity = 414/460 (90.00%), Postives = 427/460 (92.83%), Query Frame = 0

Query: 1   MVPFFLFIFIFFTTLFPSPLVIADLPTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60
           M+PF LFIFI+F TL PSP  IA L TCSGIVPM YRNDKISI DFGGIGDGRTLNT+AF
Sbjct: 1   MLPFSLFIFIYFATLLPSPFAIAQLTTCSGIVPMNYRNDKISITDFGGIGDGRTLNTKAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVFLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120
           RAAIYRIQHL+RRGGTLLYIPPGV+LTESFNLTSHMTLYLAK AVIKAVQ SSNWPV+AP
Sbjct: 61  RAAIYRIQHLKRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQGSSNWPVVAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV 180
           LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV 180

Query: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPALYILESN 240
           EFINSY+IIISNV FLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDG+DP        
Sbjct: 181 EFINSYDIIISNVRFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDP-------- 240

Query: 241 KPCKLMSTDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFA 300
                   DSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRR+SGSSPFA
Sbjct: 241 --------DSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFA 300

Query: 301 GVAVGSETSGGVENVLAEHLNFYDMGVGINIKTNIGRGGFIRNITVSNVYMESSRKGLKI 360
           GVAVGSETSGGV NVLAEHLNFYDMG+GINIKTNIGRGGFI+NITVSNVYME SRKGLKI
Sbjct: 301 GVAVGSETSGGVANVLAEHLNFYDMGIGINIKTNIGRGGFIKNITVSNVYMERSRKGLKI 360

Query: 361 AGDTGDHPDDKYDPNALPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNINLRGA 420
           AGDTGDHPDDK+DPNALPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNI+LRGA
Sbjct: 361 AGDTGDHPDDKFDPNALPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNIHLRGA 420

Query: 421 TRPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDGLCSDN 461
            RPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDG CS N
Sbjct: 421 MRPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDGSCSAN 444

BLAST of HG10009059 vs. ExPASy Swiss-Prot
Match: A7PZL3 (Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 4.0e-113
Identity = 202/425 (47.53%), Postives = 277/425 (65.18%), Query Frame = 0

Query: 37  RNDKISIADFGGIGDGRTLNTRAFRAAIYRIQHLRRRGGTLLYIPPGVFLTESFNLTSHM 96
           R    S+ DFGG+GDG+TLNT+AF+ A+  +      GG  LY+P G +LT SF+LTSH 
Sbjct: 59  RAHSASLVDFGGVGDGQTLNTKAFQDAVSELSKYGSEGGAQLYVPAGKWLTGSFSLTSHF 118

Query: 97  TLYLAKGAVIKAVQDSSNWPVIAPLPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTI 156
           TL+L + AV+ A QD S WPVI PLPSYGRGR+   GRY S I G  + DV+ITG+NGTI
Sbjct: 119 TLFLHRDAVLLASQDISQWPVIKPLPSYGRGRDAAAGRYTSLIFGTNLTDVIITGDNGTI 178

Query: 157 DGQGDAWWNMWRTGTLKFTRPNLVEFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYV 216
           DGQG  WW  +  G LK+TRP L+E + S +I ISN+  LNSP WN+HPVY  N++++ +
Sbjct: 179 DGQGGLWWQRFHGGKLKYTRPYLIELMYSADIQISNLTLLNSPSWNVHPVYSRNILIQGI 238

Query: 217 TILAPRDSPNTDGVDPALYILESNKPCKLMSTDSSNNVCIEDSYISTGDDLVAVKSGWDE 276
           TILAP  SPNTDG++P                DS  N  IED YI +GDD VAVKSGWDE
Sbjct: 239 TILAPVRSPNTDGINP----------------DSCTNTRIEDCYIVSGDDCVAVKSGWDE 298

Query: 277 YGIAYGRSSYDITIRRISGSSPFAGV-AVGSETSGGVENVLAEHLNFYDMGVGINIKTNI 336
           YGIAYG  +  + IRR++  SP++ V A+GSE SGG+++V AE +   +   GI IKT I
Sbjct: 299 YGIAYGMPTKQLVIRRLTCISPYSAVIALGSEMSGGIQDVRAEDIVAINSESGIRIKTGI 358

Query: 337 GRGGFIRNITVSNVYMESSRKGLKIAGDTGDHPDDKYDPNALPIVKDITIKNVWGVNVQQ 396
           GRGG++++I V  + M++ +    + G+ G H D+ YDP A P+++ I  +++   NV  
Sbjct: 359 GRGGYVKDIYVRGMTMKTMKWAFWMTGNYGSHADNHYDPKAFPVIQGINYRDMVAENVSM 418

Query: 397 AGSIYGLRNSPFTGICLSNINLRGATRPRSVPWTCSYVSGAALLVSPWPCSEL--TSTQQ 456
           A  + G+ + PFTGIC+SN+ +  A + + VPWTC+ V G +  V+P PCS L     ++
Sbjct: 419 AARLEGIPSDPFTGICISNVTIHLAAKAKKVPWTCTDVEGISSGVTPTPCSTLPDQGPEK 467

Query: 457 DGLCS 459
             LC+
Sbjct: 479 TSLCN 467

BLAST of HG10009059 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 121.3 bits (303), Expect = 2.8e-26
Identity = 103/391 (26.34%), Postives = 176/391 (45.01%), Query Frame = 0

Query: 41  ISIADFGGIGDGRTLNTRAFRAAIYRIQHLRRRGGTLLYIPPG-VFLTESFNLTS--HMT 100
           +S++DFG  GDG+T +T+AF  A  +       G   L +P G  +L +S  LT   +  
Sbjct: 68  VSVSDFGAKGDGKTDDTQAFVNAWKKA--CSSNGAVNLLVPKGNTYLLKSIQLTGPCNSI 127

Query: 101 LYLAKGAVIKAVQDSSNWPVIAPLPSYGRGRERPGGRYVSFIHGDGVHDVVIT-GENGTI 160
           L +     + A Q  S++  I+              +++ F   DGV+++ +  G+ G +
Sbjct: 128 LTVQIFGTLSASQKRSDYKDIS--------------KWIMF---DGVNNLSVDGGDTGVV 187

Query: 161 DGQGDAWW-NMWRTGTLK--FTRPNLVEFINSYNIIISNVMFLNSPFWNIHPVYCSNVVV 220
           DG G+ WW N  +    K     P  + F NS ++I+ N+   N+    I    CSNV V
Sbjct: 188 DGNGETWWQNSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQV 247

Query: 221 RYVTILAPRDSPNTDGVDPALYILESNKPCKLMSTDSSNNVCIEDSYISTGDDLVAVKSG 280
             V + AP DSPNTDG+                   ++ N+ + +S I TGDD ++++SG
Sbjct: 248 SNVVVTAPADSPNTDGI----------------HITNTQNIRVSESIIGTGDDCISIESG 307

Query: 281 WDEYGIAYGRSSYDITIRRISGSSPFAGVAVGS----ETSGGVENVLAEHLNFYDMGVGI 340
                      S ++ I  I+   P  G+++GS     +   V  V  +         G+
Sbjct: 308 -----------SQNVQINDIT-CGPGHGISIGSLGDDNSKAFVSGVTVDGAKLSGTDNGV 367

Query: 341 NIKTNIGRGGFIRNITVSNVYMESSRKGLKIAGDTGDHPDDKYDPNALPIVKDITIKNVW 400
            IKT  G  G   NI   N+ M++ +  + I  D  D      + +A+  VK++  +++ 
Sbjct: 368 RIKTYQGGSGTASNIIFQNIQMDNVKNPIIIDQDYCDKSKCTTEKSAVQ-VKNVVYRDIS 410

Query: 401 GVNVQQAGSIYGL-RNSPFTGICLSNINLRG 420
           G +  +    +   +N P  GI L  +N++G
Sbjct: 428 GTSASENAITFNCSKNYPCQGIVLDRVNIKG 410

BLAST of HG10009059 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 7.2e-22
Identity = 113/429 (26.34%), Postives = 181/429 (42.19%), Query Frame = 0

Query: 38  NDKISIADFGGIGDGRTLNTRAFRAAIYRIQHLRRRGGTLLYIPPGVFLTESFNLTSHMT 97
           ++ + +  FG +GDG T +++AF  A   +      G     +P G            MT
Sbjct: 21  SNALDVTQFGAVGDGVTDDSQAFLKAWEAV--CSGTGDGQFVVPAG------------MT 80

Query: 98  LYLAKGAVIKAVQDSSNWPVIAPL------PSYGRGRERPGGRYVSFIHGDGVHDVVITG 157
             L     +K      + PV   +      PS G  +    G    +I    +  +VI G
Sbjct: 81  FMLQP---LKFQGSCKSTPVFVQMLGKLVAPSKGNWK----GDKDQWILFTDIEGLVIEG 140

Query: 158 ENGTIDGQGDAWWNMWRTGTLKFTRPNLVEFINSYNIIISNVMFLNSPFWNIHPVYCSNV 217
           + G I+GQG +WW        K +RP  ++F +  N+ +S +  L+SP  +IH   C+ V
Sbjct: 141 D-GEINGQGSSWWEH------KGSRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYV 200

Query: 218 VVRYVTILAPRDSPNTDGVDPALYILESNKPCKLMSTDSSNNVCIEDSYISTGDDLVAVK 277
            +  + I AP  SPNTDG+D                  +S+NV I+D  I+TGDD +A+ 
Sbjct: 201 TISSLRINAPESSPNTDGID----------------VGASSNVVIQDCIIATGDDCIAIN 260

Query: 278 SGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGG----VENVLAEHLNFYDMGV 337
           SG     I    S  D          P  G+++GS    G    VENV  ++ NF     
Sbjct: 261 SGTSNIHI----SGID--------CGPGHGISIGSLGKDGETATVENVCVQNCNFRGTMN 320

Query: 338 GINIKTNIGRGGFIRNITVSNVYMESSRKGLKIAG--DTGDHPDDKYDPNALPIVKDITI 397
           G  IKT  G  G+ R IT + + +++    + I    + GD  + K   ++   V  +  
Sbjct: 321 GARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKVVF 380

Query: 398 KNVWGVNVQQAGSIYGL-RNSPFTGICLSNINLRGAT--RPRSVPWTCSYVSGAALLVSP 451
            N  G +  + G  +      P T I L ++ +  A+    +     C  V GA+ +  P
Sbjct: 381 SNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGSGQVAQGQCLNVRGASTIAVP 393

BLAST of HG10009059 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.8e-17
Identity = 109/402 (27.11%), Postives = 166/402 (41.29%), Query Frame = 0

Query: 37  RNDKISIAD---FGGIGDGRTLNTRAFRAAIYRIQHLRRRGGTLLYIPPG---VFLTESF 96
           R+D I+I +   +G +GDG+   T AF  A    Q   ++   +L +P     V     F
Sbjct: 52  RHDAINIFNVEKYGAVGDGKHDCTEAFSTA---WQAACKKPSAMLLVPGNKKFVVNNLFF 111

Query: 97  NLTSHMTLYLAKGAVIKAVQDSSNWPVIAPLPSYGRGRERPGGRYVSFIHGDGVHDVVIT 156
           N             +I A Q+ ++W              +    ++ F    G     + 
Sbjct: 112 NGPCQPHFTFKVDGIIAAYQNPASW--------------KNNRIWLQFAKLTG---FTLM 171

Query: 157 GENGTIDGQGDAWWN---MWRTG---TLKFTRPNLVEFINSYNIIISNVMFLNSPFWNIH 216
           G+ G IDGQG  WW     W  G        RP  ++F  S  +II  +  +NSP +++ 
Sbjct: 172 GK-GVIDGQGKQWWAGQCKWVNGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLV 231

Query: 217 PVYCSNVVVRYVTILAPRDSPNTDGVDPALYILESNKPCKLMSTDSSNNVCIEDSYISTG 276
              C  V +  ++I APRDSPNTDG+D                  +S N  ++ + I TG
Sbjct: 232 FGNCEGVKIIGISITAPRDSPNTDGID----------------IFASKNFHLQKNTIGTG 291

Query: 277 DDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVENVLAE----HL 336
           DD VA+           G  S +I I  +    P  G+++GS    G EN  AE    H+
Sbjct: 292 DDCVAI-----------GTGSSNIVIEDLI-CGPGHGISIGSL---GRENSRAEVSYVHV 351

Query: 337 N---FYDMGVGINIKTNIGRGGFIRNITVSNVYMESSRKGLKIAGDTGDHPDDKYDPNAL 396
           N   F D   G+ IKT  G  G   +I   NV M +S   + I            +  + 
Sbjct: 352 NGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCTSASACQNQRSA 401

Query: 397 PIVKDITIKNVWGVNVQQAGSIYGLRNS-PFTGICLSNINLR 419
             ++D+T KN+ G +   A       +S P   I LS+I+L+
Sbjct: 412 VQIQDVTYKNIRGTSATAAAIQLKCSDSMPCKDIKLSDISLK 401

BLAST of HG10009059 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 3.1e-17
Identity = 79/276 (28.62%), Postives = 118/276 (42.75%), Query Frame = 0

Query: 154 GTIDGQGDAWWN------MWRTGTLKFTRPNLVEFINSYNIIISNVMFLNSPFWNIHPVY 213
           G IDGQG  WW         RT      RP  ++   S ++ +  +  +NSP +++    
Sbjct: 154 GVIDGQGQQWWAGQCKVVNGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGE 213

Query: 214 CSNVVVRYVTILAPRDSPNTDGVDPALYILESNKPCKLMSTDSSNNVCIEDSYISTGDDL 273
           C  V ++ + I APRDSPNTDG+D                  +S    IE   I TGDD 
Sbjct: 214 CEGVKIQGLKIKAPRDSPNTDGID----------------IFASKRFHIEKCVIGTGDDC 273

Query: 274 VAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGS----ETSGGVENVLAEHLNFY 333
           +A+           G  S +ITI+ +    P  G+++GS     +   V +V      F 
Sbjct: 274 IAI-----------GTGSSNITIKDLI-CGPGHGISIGSLGRDNSRAEVSHVHVNRAKFI 333

Query: 334 DMGVGINIKTNIGRGGFIRNITVSNVYMESSRKGLKIAGDTGDHPDDKYDPNALPIVKDI 393
           D   G+ IKT  G  G    IT  NV M +S   + I            +  +   ++ +
Sbjct: 334 DTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTSASACQNQRSAVQIQGV 393

Query: 394 TIKNVWGVNVQQAGSIYGLRNS-PFTGICLSNINLR 419
           T KN+ G +   A       +S P TGI LSN++L+
Sbjct: 394 TYKNIHGTSATAAAIQLMCSDSVPCTGIQLSNVSLK 401

BLAST of HG10009059 vs. ExPASy TrEMBL
Match: A0A5A7TLS6 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001910 PE=3 SV=1)

HSP 1 Score: 873.2 bits (2255), Expect = 4.7e-250
Identity = 425/461 (92.19%), Postives = 436/461 (94.58%), Query Frame = 0

Query: 1   MVPFFLFIFIFFTTLFPSPLVIADLPTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60
           MVPF LF+FIFFTTLFPSPL +ADL TCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF
Sbjct: 1   MVPFSLFVFIFFTTLFPSPLAVADLITCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVFLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120
           RAAIYRIQHLRRRGGTLLYIPPGV+LTE+FNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTETFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV 180
           LPSYGRGRERPGGRYVSFIHG GVHDVVITGENGTIDGQGDAWWNMWRTGTLK+TRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGHGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLV 180

Query: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPALYILESN 240
           EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDP        
Sbjct: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDP-------- 240

Query: 241 KPCKLMSTDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFA 300
                   DSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFA
Sbjct: 241 --------DSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFA 300

Query: 301 GVAVGSETSGGVENVLAEHLNFYDMGVGINIKTNIGRGGFIRNITVSNVYMESSRKGLKI 360
           GVAVGSETSGGV NVLAEHLNFYDMGVGINIKTNIGRGGFI+NITVSNVYME+SRKGLKI
Sbjct: 301 GVAVGSETSGGVANVLAEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKI 360

Query: 361 AGDTGDHPDDKYDPNALPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNINLRGA 420
           AGDTGDHPDDK+DPNALPIVKDITIKN WGV VQQAGSIYGLR+SPFTGICLSNIN RG 
Sbjct: 361 AGDTGDHPDDKFDPNALPIVKDITIKNAWGVKVQQAGSIYGLRDSPFTGICLSNINFRGT 420

Query: 421 TRPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDGLCSDNF 462
           TRPRSVPWTCSYVSGAAL+VSPWPCSELTST+QDGLCSDNF
Sbjct: 421 TRPRSVPWTCSYVSGAALMVSPWPCSELTSTEQDGLCSDNF 445

BLAST of HG10009059 vs. ExPASy TrEMBL
Match: A0A1S3ATJ9 (probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103482817 PE=3 SV=1)

HSP 1 Score: 873.2 bits (2255), Expect = 4.7e-250
Identity = 425/461 (92.19%), Postives = 436/461 (94.58%), Query Frame = 0

Query: 1   MVPFFLFIFIFFTTLFPSPLVIADLPTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60
           MVPF LF+FIFFTTLFPSPL +ADL TCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF
Sbjct: 1   MVPFSLFVFIFFTTLFPSPLAVADLITCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVFLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120
           RAAIYRIQHLRRRGGTLLYIPPGV+LTE+FNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTETFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV 180
           LPSYGRGRERPGGRYVSFIHG GVHDVVITGENGTIDGQGDAWWNMWRTGTLK+TRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGHGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLV 180

Query: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPALYILESN 240
           EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDP        
Sbjct: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDP-------- 240

Query: 241 KPCKLMSTDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFA 300
                   DSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFA
Sbjct: 241 --------DSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFA 300

Query: 301 GVAVGSETSGGVENVLAEHLNFYDMGVGINIKTNIGRGGFIRNITVSNVYMESSRKGLKI 360
           GVAVGSETSGGV NVLAEHLNFYDMGVGINIKTNIGRGGFI+NITVSNVYME+SRKGLKI
Sbjct: 301 GVAVGSETSGGVANVLAEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKI 360

Query: 361 AGDTGDHPDDKYDPNALPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNINLRGA 420
           AGDTGDHPDDK+DPNALPIVKDITIKN WGV VQQAGSIYGLR+SPFTGICLSNIN RG 
Sbjct: 361 AGDTGDHPDDKFDPNALPIVKDITIKNAWGVKVQQAGSIYGLRDSPFTGICLSNINFRGT 420

Query: 421 TRPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDGLCSDNF 462
           TRPRSVPWTCSYVSGAAL+VSPWPCSELTST+QDGLCSDNF
Sbjct: 421 TRPRSVPWTCSYVSGAALMVSPWPCSELTSTEQDGLCSDNF 445

BLAST of HG10009059 vs. ExPASy TrEMBL
Match: A0A0A0KK36 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G150430 PE=3 SV=1)

HSP 1 Score: 866.7 bits (2238), Expect = 4.4e-248
Identity = 421/461 (91.32%), Postives = 434/461 (94.14%), Query Frame = 0

Query: 1   MVPFFLFIFIFFTTLFPSPLVIADLPTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60
           M+PF LFIFIFFTTLFPSPL +ADL TCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF
Sbjct: 1   MLPFPLFIFIFFTTLFPSPLAVADLTTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVFLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120
           RAAIYRIQHLRRRGGTLLYIPPGV+LTE+FNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTETFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV 180
           LPSYGRGRERPGGRY+S IHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLK+TRP+LV
Sbjct: 121 LPSYGRGRERPGGRYISLIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPSLV 180

Query: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPALYILESN 240
           EF+NSYNIIISNVMFLNSPFWNIHPVYC NVVVRYVTILAPRDSPNTDGVDP        
Sbjct: 181 EFVNSYNIIISNVMFLNSPFWNIHPVYCRNVVVRYVTILAPRDSPNTDGVDP-------- 240

Query: 241 KPCKLMSTDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFA 300
                   DSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGR SYDITIRRISGSSPFA
Sbjct: 241 --------DSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRCSYDITIRRISGSSPFA 300

Query: 301 GVAVGSETSGGVENVLAEHLNFYDMGVGINIKTNIGRGGFIRNITVSNVYMESSRKGLKI 360
           GVAVGSE SGGV NVLAEHLNFYDMGVGINIKTNIGRGGFI+NITVSNVYME+SRKGLKI
Sbjct: 301 GVAVGSEASGGVANVLAEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKI 360

Query: 361 AGDTGDHPDDKYDPNALPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNINLRGA 420
           AGD GDHPDDK+DPNALPIVKDITIKN+WGVNVQQAGSIYGLR+SPFTGICLSNINLRGA
Sbjct: 361 AGDAGDHPDDKFDPNALPIVKDITIKNIWGVNVQQAGSIYGLRDSPFTGICLSNINLRGA 420

Query: 421 TRPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDGLCSDNF 462
           TRPRSVPWTCSYVSGAA LVSPWPCSELTST QDGLCSDNF
Sbjct: 421 TRPRSVPWTCSYVSGAASLVSPWPCSELTSTDQDGLCSDNF 445

BLAST of HG10009059 vs. ExPASy TrEMBL
Match: A0A6J1I1Q1 (probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111469049 PE=3 SV=1)

HSP 1 Score: 851.7 bits (2199), Expect = 1.5e-243
Identity = 415/460 (90.22%), Postives = 428/460 (93.04%), Query Frame = 0

Query: 1   MVPFFLFIFIFFTTLFPSPLVIADLPTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60
           M+PF LFIFI+F TL PSP  IA L TCSGIVPM YRNDKISI DFGGIGDGRTLNT+AF
Sbjct: 1   MLPFSLFIFIYFATLLPSPFAIAQLTTCSGIVPMNYRNDKISITDFGGIGDGRTLNTKAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVFLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120
           RAAIYRIQ+L+RRGGTLLYIPPGV+LTESFNLTSHMTLYLAK AVIKAVQ SSNWPV+AP
Sbjct: 61  RAAIYRIQNLKRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQGSSNWPVVAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV 180
           LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV 180

Query: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPALYILESN 240
           EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDG+DP        
Sbjct: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDP-------- 240

Query: 241 KPCKLMSTDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFA 300
                   DSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRR+SGSSPFA
Sbjct: 241 --------DSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFA 300

Query: 301 GVAVGSETSGGVENVLAEHLNFYDMGVGINIKTNIGRGGFIRNITVSNVYMESSRKGLKI 360
           GVAVGSETSGGV NVLAEHLNFYDMG+GINIKTNIGRGGFI+NITVSNVYME SRKGLKI
Sbjct: 301 GVAVGSETSGGVANVLAEHLNFYDMGIGINIKTNIGRGGFIKNITVSNVYMERSRKGLKI 360

Query: 361 AGDTGDHPDDKYDPNALPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNINLRGA 420
           AGDTGDHPDDK+DPNALPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNI+LRGA
Sbjct: 361 AGDTGDHPDDKFDPNALPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNIHLRGA 420

Query: 421 TRPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDGLCSDN 461
            RPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDG CS N
Sbjct: 421 MRPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDGSCSAN 444

BLAST of HG10009059 vs. ExPASy TrEMBL
Match: A0A6J1EMW9 (probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111435810 PE=3 SV=1)

HSP 1 Score: 846.3 bits (2185), Expect = 6.1e-242
Identity = 413/460 (89.78%), Postives = 425/460 (92.39%), Query Frame = 0

Query: 1   MVPFFLFIFIFFTTLFPSPLVIADLPTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60
           M+PF LFIFI   TL PSP  IA L TCSGIVPM YRNDKISI DFGGIGDGRTLNT+AF
Sbjct: 1   MLPFSLFIFIHLATLLPSPFAIAQLTTCSGIVPMNYRNDKISITDFGGIGDGRTLNTKAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVFLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120
           RAAIYRIQHL+RRGGTLLYIPPGV+LTESFNLTSHMTLYLAK AVIKAVQ SSNWPV+AP
Sbjct: 61  RAAIYRIQHLKRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQGSSNWPVVAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV 180
           LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV 180

Query: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPALYILESN 240
           EFINSY+IIISNV FLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDG+DP        
Sbjct: 181 EFINSYDIIISNVRFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDP-------- 240

Query: 241 KPCKLMSTDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFA 300
                   DSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRR+SGSSPFA
Sbjct: 241 --------DSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFA 300

Query: 301 GVAVGSETSGGVENVLAEHLNFYDMGVGINIKTNIGRGGFIRNITVSNVYMESSRKGLKI 360
           GVAVGSETSGGV NVLAEHLNFYDMG+GINIKTNIGRGGFI+NITVSNVYME SRKGLKI
Sbjct: 301 GVAVGSETSGGVANVLAEHLNFYDMGIGINIKTNIGRGGFIKNITVSNVYMERSRKGLKI 360

Query: 361 AGDTGDHPDDKYDPNALPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNINLRGA 420
           AGDTGDHPDDK+DPNALPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNI+LRGA
Sbjct: 361 AGDTGDHPDDKFDPNALPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNIHLRGA 420

Query: 421 TRPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDGLCSDN 461
           TRPRSVPWTCSYVSGAALLVSPWPCS LTSTQQDG CS N
Sbjct: 421 TRPRSVPWTCSYVSGAALLVSPWPCSALTSTQQDGSCSAN 444

BLAST of HG10009059 vs. TAIR 10
Match: AT4G23820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 677.9 bits (1748), Expect = 5.6e-195
Identity = 326/458 (71.18%), Postives = 383/458 (83.62%), Query Frame = 0

Query: 6   LFIFIF-FTTLFPSPLVIADL-PTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAFRAA 65
           L + IF F+ LF S   + D   TCSGIVP++YR DKISI DFGG+GDGRT+NT+AFRAA
Sbjct: 4   LSVSIFLFSCLFVSSSSLGDSEATCSGIVPLRYRYDKISITDFGGVGDGRTVNTKAFRAA 63

Query: 66  IYRIQHLRRRGGTLLYIPPGVFLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAPLPS 125
           IYRIQHL+RRGGTLLYIPPGV+LTESFNLTSHMTLYLAKGAVI+AVQD+ NWP+I PLPS
Sbjct: 64  IYRIQHLKRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIRAVQDTWNWPLIDPLPS 123

Query: 126 YGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLVEFI 185
           YGRGRE PGGRY+SFIHGDG+ DVVITG+NGTIDGQG+ WWNMWR+ TLK+TRPNL+EF 
Sbjct: 124 YGRGRELPGGRYMSFIHGDGLRDVVITGQNGTIDGQGEVWWNMWRSRTLKYTRPNLIEFK 183

Query: 186 NSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPALYILESNKPC 245
           +S  IIISNV+F NSPFWNIHPVYCSNVV+ +VTILAP+DSPNTDG+DP           
Sbjct: 184 DSKEIIISNVIFQNSPFWNIHPVYCSNVVIHHVTILAPQDSPNTDGIDP----------- 243

Query: 246 KLMSTDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVA 305
                DSS NVCIEDSYISTGDDLVA+KSGWD+YGIAYGR S +ITIRRI+GSSPFAG+A
Sbjct: 244 -----DSSYNVCIEDSYISTGDDLVAIKSGWDQYGIAYGRPSSNITIRRITGSSPFAGIA 303

Query: 306 VGSETSGGVENVLAEHLNFYDMGVGINIKTNIGRGGFIRNITVSNVYMESSRKGLKIAGD 365
           +GSETSGG++N++AEH+   +MGVG+NIKTNIGRGG+I+NI +S+VY+++++ G+KIAGD
Sbjct: 304 IGSETSGGIKNIIAEHITLSNMGVGVNIKTNIGRGGYIKNIKISDVYVDTAKYGIKIAGD 363

Query: 366 TGDHPDDKYDPNALPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNINLRGATRP 425
           TGDHPD+ Y+PNALP+VK I IKNVWGVNV+ AGSI GL+ SPFTGICLS INL G+   
Sbjct: 364 TGDHPDENYNPNALPVVKGIHIKNVWGVNVRNAGSIQGLKGSPFTGICLSEINLHGSLNS 423

Query: 426 RSVPWTCSYVSGAALLVSPWPCSELTSTQQDGLCSDNF 462
               W CS VSG +L VSPWPCSEL +T    LCS  F
Sbjct: 424 YKT-WKCSDVSGTSLKVSPWPCSELRTTGGSNLCSSTF 444

BLAST of HG10009059 vs. TAIR 10
Match: AT5G41870.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 607.4 bits (1565), Expect = 9.2e-174
Identity = 294/459 (64.05%), Postives = 358/459 (78.00%), Query Frame = 0

Query: 3   PFFLFIFIFFTTLFPSPLVIADLPTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAFRA 62
           P  +F+    +T+ P  L +    TCSGIVPMK+RN+ +SI+DFG +GDG+TLNT+AF +
Sbjct: 7   PISVFVLFLLSTVLPHHLSLGAPITCSGIVPMKHRNEMLSISDFGAVGDGKTLNTKAFNS 66

Query: 63  AIYRIQHLRRRG-GTLLYIPPGVFLTESFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAPL 122
           AI RI++      GTLLY+P GV+LT+SFNLTSHMTLYLA GAVIKAVQD+  WP+  PL
Sbjct: 67  AIDRIRNSNNSNEGTLLYVPRGVYLTQSFNLTSHMTLYLADGAVIKAVQDTEKWPLTDPL 126

Query: 123 PSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLVE 182
           PSYGRGRE PG RY+SFIHGDG++DVVITG NGTIDGQG+ WWNMWR GTLKFTRP L+E
Sbjct: 127 PSYGRGREHPGRRYISFIHGDGLNDVVITGRNGTIDGQGEPWWNMWRHGTLKFTRPGLIE 186

Query: 183 FINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPALYILESNK 242
           F NS NI++S+V+  NSPFW +HPVYCSNVVV +VTILAP DS NTDG+DP         
Sbjct: 187 FNNSTNILVSHVVLQNSPFWTLHPVYCSNVVVHHVTILAPTDSYNTDGIDP--------- 246

Query: 243 PCKLMSTDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAG 302
                  DSS+NVCIEDSYISTGDDLVAVKSGWDEYGIAY R S DITIRRI+GSSPFAG
Sbjct: 247 -------DSSSNVCIEDSYISTGDDLVAVKSGWDEYGIAYNRPSRDITIRRITGSSPFAG 306

Query: 303 VAVGSETSGGVENVLAEHLNFYDMGVGINIKTNIGRGGFIRNITVSNVYMESSRKGLKIA 362
           +A+GSETSGG++NV  E++  Y+ G+GI+IKTNIGRGG I+ IT+S VY+E  R G+KI+
Sbjct: 307 IAIGSETSGGIQNVTVENITLYNSGIGIHIKTNIGRGGSIQGITISGVYLEKVRTGIKIS 366

Query: 363 GDTGDHPDDKYDPNALPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNINLRGAT 422
           GDTGDHPDDK++ +ALPIV+ ITIKNVWG+ V++AG + GL++SPFT +C SN+ L G  
Sbjct: 367 GDTGDHPDDKFNTSALPIVRGITIKNVWGIKVERAGMVQGLKDSPFTNLCFSNVTLTGT- 426

Query: 423 RPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDGLCSDN 461
             RS  W CS V GAA  V+P PC EL++T Q G   +N
Sbjct: 427 -KRSPIWKCSDVVGAADKVNPTPCPELSATTQQGGSCEN 447

BLAST of HG10009059 vs. TAIR 10
Match: AT3G62110.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 438.3 bits (1126), Expect = 7.4e-123
Identity = 210/408 (51.47%), Postives = 278/408 (68.14%), Query Frame = 0

Query: 36  YRNDKISIADFGGIGDGRTLNTRAFRAAIYRIQHLRRRGGTLLYIPPGVFLTESFNLTSH 95
           +R   +SI +FG +GDG TLNT+AF+ A++ +     +GG  L++P G +LT SF+L SH
Sbjct: 38  HRPHSVSITEFGAVGDGVTLNTKAFQNALFYLNSFSDKGGAKLFVPAGQWLTGSFDLISH 97

Query: 96  MTLYLAKGAVIKAVQDSSNWPVIAPLPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGT 155
           +TL+L KGA I     S NWPV+ PLPSYGRGRE PG R+ S I+G  + DVVITGENGT
Sbjct: 98  LTLWLDKGATILGSTSSENWPVVDPLPSYGRGRELPGRRHRSLIYGQNLTDVVITGENGT 157

Query: 156 IDGQGDAWWNMWRTGTLKFTRPNLVEFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRY 215
           IDGQG  WW+ +R G L +TRP+LVE +NS  +IISN+ FLNSPFWNIHPVYC +VVV+ 
Sbjct: 158 IDGQGTVWWDWFRNGELNYTRPHLVELMNSTGLIISNLTFLNSPFWNIHPVYCRDVVVKN 217

Query: 216 VTILAPRDSPNTDGVDPALYILESNKPCKLMSTDSSNNVCIEDSYISTGDDLVAVKSGWD 275
           +TILAP +SPNTDGVDP                DSS NVCIED YI TGDDLV++KSGWD
Sbjct: 218 LTILAPLESPNTDGVDP----------------DSSTNVCIEDCYIVTGDDLVSIKSGWD 277

Query: 276 EYGIAYGRSSYDITIRRISG-SSPFAGVAVGSETSGGVENVLAEHLNFYDMGVGINIKTN 335
           EYGI+Y R S  I I R++G ++  +G+A+GSE SGGV  +  + L+ ++   GI IKT+
Sbjct: 278 EYGISYARPSSKIKINRLTGQTTSSSGIAIGSEMSGGVSEIYIKDLHLFNSNTGIRIKTS 337

Query: 336 IGRGGFIRNITVSNVYMESSRKGLKIAGDTGDHPDDKYDPNALPIVKDITIKNVWGVNVQ 395
            GRGG++RN+ + NV +++ +K ++  G  G+HPD+KYDP ALP ++ IT +NV G  + 
Sbjct: 338 AGRGGYVRNVHILNVKLDNVKKAIRFTGKYGEHPDEKYDPKALPAIEKITFENVNGDGIG 397

Query: 396 QAGSIYGLRNSPFTGICLSNINLRGATRPRSVPWTCSYVSGAALLVSP 443
            AG + G+    F  IC  N+ LR     +  PW CS V G +  VSP
Sbjct: 398 VAGLLEGIEGDVFKNICFLNVTLRVKKNSKKSPWECSNVRGYSQWVSP 429

BLAST of HG10009059 vs. TAIR 10
Match: AT4G33440.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 434.9 bits (1117), Expect = 8.2e-122
Identity = 227/464 (48.92%), Postives = 297/464 (64.01%), Query Frame = 0

Query: 3   PFFLFIFIFFTTLFPSPLVIA---------------DLPTCSGIVPMKYRNDKI--SIAD 62
           P + F+ + FT L    L I+               D  TCSG        ++I  SI D
Sbjct: 17  PSWAFLLLVFTVLAILSLQISSNSFLPLWIPTSQYDDPVTCSGFFNHDPFPNRIVMSITD 76

Query: 63  FGGIGDGRTLNTRAFRAAIYRIQHLRRRGGTLLYIPPGVFLTESFNLTSHMTLYLAKGAV 122
           FGG+GDG+T NT AFR A+  ++     GG  L +P G +L+ SFNLTS+ TL+L +GA+
Sbjct: 77  FGGVGDGKTSNTAAFRRAVRHLEGFAAEGGAQLNVPEGTWLSGSFNLTSNFTLFLERGAL 136

Query: 123 IKAVQDSSNWPVIAPLPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWN 182
           I   +D   WP+I PLPSYGRGRERPGGR++S IHGD + +VVITGENGTIDGQG  WW 
Sbjct: 137 ILGSKDLDEWPIIEPLPSYGRGRERPGGRHISLIHGDNLTNVVITGENGTIDGQGKMWWE 196

Query: 183 MWRTGTLKFTRPNLVEFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSP 242
           +W   TL  TR +L+E  NS+NI+ISN+  LNSPFW IHPVYCSNVV+R +TILAP ++P
Sbjct: 197 LWWNRTLVHTRGHLIELKNSHNILISNLTLLNSPFWTIHPVYCSNVVIRNMTILAPMNAP 256

Query: 243 NTDGVDPALYILESNKPCKLMSTDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSS 302
           NTDG+DP                DSS NVCIED YI +GDDLVAVKSGWD+YG+A  R S
Sbjct: 257 NTDGIDP----------------DSSTNVCIEDCYIESGDDLVAVKSGWDQYGMAVARPS 316

Query: 303 YDITIRRISGSS-PFAGVAVGSETSGGVENVLAEHLNFYDMGVGINIKTNIGRGGFIRNI 362
            +I IRRISG++   +GV +GSE SGG+ N+  E ++ +D   G+ IKT+ GRGG+I NI
Sbjct: 317 SNIVIRRISGTTRTCSGVGIGSEMSGGIFNITVEDIHVWDSAAGLRIKTDKGRGGYISNI 376

Query: 363 TVSNVYMESSRKGLKIAGDTGDHPDDKYDPNALPIVKDITIKNVWGVNVQQAGSIYGLRN 422
           T +NV +E  +  ++ +  + DH DDK+DP ALP VK I I NV  +N ++A  + G+  
Sbjct: 377 TFNNVLLEKVKVPIRFSSGSNDHSDDKWDPKALPRVKGIYISNVVSLNSRKAPMLLGVEG 436

Query: 423 SPFTGICLSNINLRGATRPRSVPWTCSYVSGAALLVSPWPCSEL 449
           + F  +CL N+ L G   P++  W C  VSG A  V P  C +L
Sbjct: 437 TSFQDVCLRNVTLLGL--PKTEKWKCKDVSGYASDVFPLSCPQL 462

BLAST of HG10009059 vs. TAIR 10
Match: AT3G16850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 410.6 bits (1054), Expect = 1.7e-114
Identity = 210/416 (50.48%), Postives = 281/416 (67.55%), Query Frame = 0

Query: 37  RNDKISIADFGGIGDGRTLNTRAFRAAIYRIQHLRRRGGTLLYIPPGVFLTESFNLTSHM 96
           R   +SI +FG +GDG+TLNT AF+ A++ ++    +GG  LY+PPG +LT SFNLTSH+
Sbjct: 36  RPHSVSILEFGAVGDGKTLNTIAFQNAVFYLKSFADKGGAQLYVPPGKWLTGSFNLTSHL 95

Query: 97  TLYLAKGAVIKAVQDSSNWPVIAPLPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTI 156
           TL+L KGA I A  D S+W V++PLPSYGRG E PG RY S I+GD + DVVITGENGT 
Sbjct: 96  TLFLEKGATILASPDPSHWDVVSPLPSYGRGIELPGKRYRSLINGDNLIDVVITGENGTF 155

Query: 157 DGQGDAWWNMWRTGTLKFTRPNLVEFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYV 216
           DGQG AWW    +G+L ++RP+++EF++S +I+ISN+ FLN+P  NIHPVYCS + +R V
Sbjct: 156 DGQGAAWWEWLESGSLNYSRPHIIEFVSSKHILISNLTFLNAPAINIHPVYCSQIHIRKV 215

Query: 217 TILAPRDSPNTDGVDPALYILESNKPCKLMSTDSSNNVCIEDSYISTGDDLVAVKSGWDE 276
            I    DSP+  GV P                DSS+NVCIEDS I+ G D V++KSGWD+
Sbjct: 216 LIETSVDSPHVLGVAP----------------DSSDNVCIEDSTINVGHDAVSLKSGWDQ 275

Query: 277 YGIAYGRSSYDITIRRISGSSPF-AGVAVGSETSGGVENVLAEHLNFYDMGVGINIKTNI 336
           YGI YGR +  + IR +   SP  AG++ GSE SGGV +V  E LN +   +G+  +T  
Sbjct: 276 YGIHYGRPTTAVHIRNLRLKSPTGAGISFGSEMSGGVSDVTVERLNIHSSLIGVAFRTTR 335

Query: 337 GRGGFIRNITVSNVYMESSRKGLKIAGDTGDHPDDKYDPNALPIVKDITIKNVWGVNVQQ 396
           GRGG+IRNIT+S+V + S    +   G TG HPDDK+D +ALP+V  I ++N  GV++  
Sbjct: 336 GRGGYIRNITISDVDLTSVDTAIVANGHTGSHPDDKFDRDALPVVTHIVMRNFTGVDIGV 395

Query: 397 AGSIYGLRNSPFTGICLSNINLRGATRPRSVPWTCSYVSGAALLVSPWPCSELTST 452
           AG++ G+  SPFT ICL++I+L+  TR     W CS VSG +  VSP PC EL S+
Sbjct: 396 AGNLTGIGESPFTSICLADIHLQ--TRSEE-SWICSNVSGFSDDVSPEPCQELMSS 432