HG10009054 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10009054 vs. NCBI nr
Match: XP_038907056.1 (probable trehalase [Benincasa hispida] >XP_038907058.1 probable trehalase [Benincasa hispida])

HSP 1 Score: 1129.8 bits (2921), Expect = 0.0e+00
Identity = 538/580 (92.76%), Postives = 560/580 (96.55%), Query Frame = 0

Query: 1   MPLIPPAAMSLPEVLSPRLVDKGPVIPVTNLVKFLERLQVAALNSFGKLDFDLKYYVDLS 60
           MPL+ PA  +LPEV SPRLVD+GPV+PVTNLVKFLERLQV ALNSFGKLDFDLKYYVDLS
Sbjct: 25  MPLLRPATATLPEVFSPRLVDRGPVVPVTNLVKFLERLQVVALNSFGKLDFDLKYYVDLS 84

Query: 61  LKFDLNSTETAFDALERSSNGSVPVENLKAFIIDYFDSAGTDLVYSEPVDFFPQPVGFLP 120
           LKFDL+STETAFDALERSSNGSV VENLK FI DYFDSAGTDLVYSEPVDF PQP+GFLP
Sbjct: 85  LKFDLDSTETAFDALERSSNGSVSVENLKTFITDYFDSAGTDLVYSEPVDFVPQPIGFLP 144

Query: 121 KVENAEVRAWALDIHNFWKNLSRRVSDDLIHRPDTHTLLPLPEPVVIPGARFREVYYWDS 180
           KVEN+EVRAWALDIHNFWKNLSRRVSDDLIHRP+THTLLPLPEPVV+PG+RFREVYYWDS
Sbjct: 145 KVENSEVRAWALDIHNFWKNLSRRVSDDLIHRPETHTLLPLPEPVVVPGSRFREVYYWDS 204

Query: 181 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 240
           YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGAR+YYTNRSQPPLLSSMVYDIYLR
Sbjct: 205 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARSYYTNRSQPPLLSSMVYDIYLR 264

Query: 241 TGDLEFVSNALPALIKEHKFWNSGFHSITVQNAPGGNGNHSLSRYYAMWNEPRPESSLLD 300
           TGDLEFV NALPALIKEHKFWNSGFHSITV+NAPGGNGNHSLSRYYAMWNEPRPESSLLD
Sbjct: 265 TGDLEFVRNALPALIKEHKFWNSGFHSITVRNAPGGNGNHSLSRYYAMWNEPRPESSLLD 324

Query: 301 VKLASKLVNNNEKQHLYREIASAAESGWDFSTRWMRDPTDLSTLATTSILPVDLNVFILK 360
            KLASK VNN EKQHLYREIASAAESGWDFS+RWMRD TDLSTLATTSILPVDLNVFILK
Sbjct: 325 EKLASKFVNNYEKQHLYREIASAAESGWDFSSRWMRDSTDLSTLATTSILPVDLNVFILK 384

Query: 361 MELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHTW 420
           MELDISNLARAVGDYCTA+HFFEASLVRKKTINSIFWNSEKGQWLDYWLDNG YKG HTW
Sbjct: 385 MELDISNLARAVGDYCTAKHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGLYKGTHTW 444

Query: 421 DARNQNQNVYASNFIPLWIESFYSDNKQMEKVLRSLRNSGLLCNAGIATSMINSGEQWDF 480
           DARNQNQNVYASNFIPLWIESFYSD++QM+KVL+SLRNSGLLCNAGIATSMINSGEQWDF
Sbjct: 445 DARNQNQNVYASNFIPLWIESFYSDSRQMKKVLKSLRNSGLLCNAGIATSMINSGEQWDF 504

Query: 481 PNGWAPIQHMIVEGLARSGLKEARALAEDIASRWLRTNYVAYKQTGYMHEKYDVQKCGDF 540
           PNGWAPIQHMIVEGLARSGLKEARALAEDIA+RWL+TNYVAYK TGYMHEKYDVQKCGDF
Sbjct: 505 PNGWAPIQHMIVEGLARSGLKEARALAEDIAARWLQTNYVAYKHTGYMHEKYDVQKCGDF 564

Query: 541 GGGGEYVPQTGFGWSNGVVLAFLEEFGWLEDQKIDCHIAS 581
           GGGGEYVPQTGFGWSNGVVLAFLEEFGW +DQ IDCH+ S
Sbjct: 565 GGGGEYVPQTGFGWSNGVVLAFLEEFGWPKDQNIDCHLTS 604

BLAST of HG10009054 vs. NCBI nr
Match: XP_008437392.1 (PREDICTED: probable trehalase [Cucumis melo])

HSP 1 Score: 1090.9 bits (2820), Expect = 0.0e+00
Identity = 522/580 (90.00%), Postives = 549/580 (94.66%), Query Frame = 0

Query: 1   MPLIPPAAMSLPEVLSPRLVDKGPVIPVTNLVKFLERLQVAALNSFGKLDFDLKYYVDLS 60
           MPL+ PAA SLPEV SPRL DKGPVIPV NLVKFLERLQV ALNSFGKLDFDLKYYVDLS
Sbjct: 25  MPLLRPAAASLPEVFSPRLADKGPVIPVPNLVKFLERLQVVALNSFGKLDFDLKYYVDLS 84

Query: 61  LKFDLNSTETAFDALERSSNGSVPVENLKAFIIDYFDSAGTDLVYSEPVDFFPQPVGFLP 120
           LKFDLNST+ AFDALERSSNGSV VENL+ FI +YF SAGTDLVYS PVDF PQP GFLP
Sbjct: 85  LKFDLNSTQIAFDALERSSNGSVSVENLRTFISNYFHSAGTDLVYSNPVDFNPQPDGFLP 144

Query: 121 KVENAEVRAWALDIHNFWKNLSRRVSDDLIHRPDTHTLLPLPEPVVIPGARFREVYYWDS 180
           KVEN EVR WALDIHNFWKNLSRRVSDDLIH PDTHTLLPLPEPVV+PG+RFRE+YYWDS
Sbjct: 145 KVENVEVRTWALDIHNFWKNLSRRVSDDLIHHPDTHTLLPLPEPVVVPGSRFREIYYWDS 204

Query: 181 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 240
           YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGAR+YYTNRSQPPLLSSMVYDIYLR
Sbjct: 205 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARSYYTNRSQPPLLSSMVYDIYLR 264

Query: 241 TGDLEFVSNALPALIKEHKFWNSGFHSITVQNAPGGNGNHSLSRYYAMWNEPRPESSLLD 300
           TGDLEFV N+LPALIKEHKFWNSGFHS+TVQN   GNGNHSLSRYYAMWNEPRPESSL+D
Sbjct: 265 TGDLEFVRNSLPALIKEHKFWNSGFHSVTVQN---GNGNHSLSRYYAMWNEPRPESSLVD 324

Query: 301 VKLASKLVNNNEKQHLYREIASAAESGWDFSTRWMRDPTDLSTLATTSILPVDLNVFILK 360
            KLASK  NN EK+HLYREIASAAESGWDFS+RWMRD TDLSTLATTSILPVDLNVFILK
Sbjct: 325 EKLASKFANNYEKKHLYREIASAAESGWDFSSRWMRDSTDLSTLATTSILPVDLNVFILK 384

Query: 361 MELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHTW 420
           MELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAH+W
Sbjct: 385 MELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHSW 444

Query: 421 DARNQNQNVYASNFIPLWIESFYSDNKQMEKVLRSLRNSGLLCNAGIATSMINSGEQWDF 480
           +ARNQNQN+YASNFIPLWIESFYSD++QM+KVL+SLRNSGLLCNAGIATS+INSGEQWDF
Sbjct: 445 NARNQNQNIYASNFIPLWIESFYSDSRQMKKVLKSLRNSGLLCNAGIATSVINSGEQWDF 504

Query: 481 PNGWAPIQHMIVEGLARSGLKEARALAEDIASRWLRTNYVAYKQTGYMHEKYDVQKCGDF 540
           PNGWAPIQHMIVEGLARS L EA+ALAEDIA+RWLRTNYVAYK TG+MHEKYDVQKCGDF
Sbjct: 505 PNGWAPIQHMIVEGLARSELPEAKALAEDIAARWLRTNYVAYKHTGFMHEKYDVQKCGDF 564

Query: 541 GGGGEYVPQTGFGWSNGVVLAFLEEFGWLEDQKIDCHIAS 581
           GGGGEYVPQTGFGWSNGVVLAFLEEFGW +DQKIDC++ S
Sbjct: 565 GGGGEYVPQTGFGWSNGVVLAFLEEFGWPKDQKIDCYLPS 601

BLAST of HG10009054 vs. NCBI nr
Match: KAA0042682.1 (putative trehalase [Cucumis melo var. makuwa] >TYK06085.1 putative trehalase [Cucumis melo var. makuwa])

HSP 1 Score: 1090.9 bits (2820), Expect = 0.0e+00
Identity = 522/580 (90.00%), Postives = 549/580 (94.66%), Query Frame = 0

Query: 1   MPLIPPAAMSLPEVLSPRLVDKGPVIPVTNLVKFLERLQVAALNSFGKLDFDLKYYVDLS 60
           MPL+ PAA SLPEV SPRL DKGPVIPV NLVKFLERLQV ALNSFGKLDFDLKYYVDLS
Sbjct: 1   MPLLRPAAASLPEVFSPRLADKGPVIPVPNLVKFLERLQVVALNSFGKLDFDLKYYVDLS 60

Query: 61  LKFDLNSTETAFDALERSSNGSVPVENLKAFIIDYFDSAGTDLVYSEPVDFFPQPVGFLP 120
           LKFDLNST+ AFDALERSSNGSV VENL+ FI +YF SAGTDLVYS PVDF PQP GFLP
Sbjct: 61  LKFDLNSTQIAFDALERSSNGSVSVENLRTFISNYFHSAGTDLVYSNPVDFNPQPDGFLP 120

Query: 121 KVENAEVRAWALDIHNFWKNLSRRVSDDLIHRPDTHTLLPLPEPVVIPGARFREVYYWDS 180
           KVEN EVR WALDIHNFWKNLSRRVSDDLIH PDTHTLLPLPEPVV+PG+RFRE+YYWDS
Sbjct: 121 KVENVEVRTWALDIHNFWKNLSRRVSDDLIHHPDTHTLLPLPEPVVVPGSRFREIYYWDS 180

Query: 181 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 240
           YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGAR+YYTNRSQPPLLSSMVYDIYLR
Sbjct: 181 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARSYYTNRSQPPLLSSMVYDIYLR 240

Query: 241 TGDLEFVSNALPALIKEHKFWNSGFHSITVQNAPGGNGNHSLSRYYAMWNEPRPESSLLD 300
           TGDLEFV N+LPALIKEHKFWNSGFHS+TVQN   GNGNHSLSRYYAMWNEPRPESSL+D
Sbjct: 241 TGDLEFVRNSLPALIKEHKFWNSGFHSVTVQN---GNGNHSLSRYYAMWNEPRPESSLVD 300

Query: 301 VKLASKLVNNNEKQHLYREIASAAESGWDFSTRWMRDPTDLSTLATTSILPVDLNVFILK 360
            KLASK  NN EK+HLYREIASAAESGWDFS+RWMRD TDLSTLATTSILPVDLNVFILK
Sbjct: 301 EKLASKFANNYEKKHLYREIASAAESGWDFSSRWMRDSTDLSTLATTSILPVDLNVFILK 360

Query: 361 MELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHTW 420
           MELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAH+W
Sbjct: 361 MELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHSW 420

Query: 421 DARNQNQNVYASNFIPLWIESFYSDNKQMEKVLRSLRNSGLLCNAGIATSMINSGEQWDF 480
           +ARNQNQN+YASNFIPLWIESFYSD++QM+KVL+SLRNSGLLCNAGIATS+INSGEQWDF
Sbjct: 421 NARNQNQNIYASNFIPLWIESFYSDSRQMKKVLKSLRNSGLLCNAGIATSVINSGEQWDF 480

Query: 481 PNGWAPIQHMIVEGLARSGLKEARALAEDIASRWLRTNYVAYKQTGYMHEKYDVQKCGDF 540
           PNGWAPIQHMIVEGLARS L EA+ALAEDIA+RWLRTNYVAYK TG+MHEKYDVQKCGDF
Sbjct: 481 PNGWAPIQHMIVEGLARSELPEAKALAEDIAARWLRTNYVAYKHTGFMHEKYDVQKCGDF 540

Query: 541 GGGGEYVPQTGFGWSNGVVLAFLEEFGWLEDQKIDCHIAS 581
           GGGGEYVPQTGFGWSNGVVLAFLEEFGW +DQKIDC++ S
Sbjct: 541 GGGGEYVPQTGFGWSNGVVLAFLEEFGWPKDQKIDCYLPS 577

BLAST of HG10009054 vs. NCBI nr
Match: XP_004143867.2 (probable trehalase [Cucumis sativus] >KGN50019.1 hypothetical protein Csa_000235 [Cucumis sativus])

HSP 1 Score: 1089.3 bits (2816), Expect = 0.0e+00
Identity = 521/580 (89.83%), Postives = 547/580 (94.31%), Query Frame = 0

Query: 1   MPLIPPAAMSLPEVLSPRLVDKGPVIPVTNLVKFLERLQVAALNSFGKLDFDLKYYVDLS 60
           MPL+ PAA SLPEV SPRL DKGPVIPV NLVKFLER+QV ALNSFGKLDFDLKYYVDLS
Sbjct: 25  MPLLRPAAASLPEVFSPRLADKGPVIPVPNLVKFLERIQVVALNSFGKLDFDLKYYVDLS 84

Query: 61  LKFDLNSTETAFDALERSSNGSVPVENLKAFIIDYFDSAGTDLVYSEPVDFFPQPVGFLP 120
           LKFDLNST+ AFDALERSSNGSV VENL+ FI +YFDSAGTDLVYS P DF P P GFLP
Sbjct: 85  LKFDLNSTQIAFDALERSSNGSVSVENLRTFISNYFDSAGTDLVYSNPADFDPHPDGFLP 144

Query: 121 KVENAEVRAWALDIHNFWKNLSRRVSDDLIHRPDTHTLLPLPEPVVIPGARFREVYYWDS 180
           KVEN EVRAWA DIHNFWKNLSRRVSDDLIH PDTHTLLPLPEPVV+PG+RFRE+YYWDS
Sbjct: 145 KVENVEVRAWAFDIHNFWKNLSRRVSDDLIHHPDTHTLLPLPEPVVVPGSRFREIYYWDS 204

Query: 181 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 240
           YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGAR+YYTNRSQPPLLSSMVYDIYLR
Sbjct: 205 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARSYYTNRSQPPLLSSMVYDIYLR 264

Query: 241 TGDLEFVSNALPALIKEHKFWNSGFHSITVQNAPGGNGNHSLSRYYAMWNEPRPESSLLD 300
           TGDLEFV N+LPALIKEHKFWNSGFHSITVQN   GNGNHSLSRYYAMWNEPRPESSL+D
Sbjct: 265 TGDLEFVRNSLPALIKEHKFWNSGFHSITVQN---GNGNHSLSRYYAMWNEPRPESSLVD 324

Query: 301 VKLASKLVNNNEKQHLYREIASAAESGWDFSTRWMRDPTDLSTLATTSILPVDLNVFILK 360
            K+ASK VNN EK+HLYREIASAAESGWDFS+RWMRD TDLSTLATTSILPVDLNVFILK
Sbjct: 325 EKVASKFVNNYEKKHLYREIASAAESGWDFSSRWMRDSTDLSTLATTSILPVDLNVFILK 384

Query: 361 MELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHTW 420
           MELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAH+W
Sbjct: 385 MELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHSW 444

Query: 421 DARNQNQNVYASNFIPLWIESFYSDNKQMEKVLRSLRNSGLLCNAGIATSMINSGEQWDF 480
           D RNQNQNVYASNFIPLW+ESFYSD++QM+KVL+SLRNSGLLCNAGIATSMINSGEQWDF
Sbjct: 445 DVRNQNQNVYASNFIPLWVESFYSDSRQMKKVLKSLRNSGLLCNAGIATSMINSGEQWDF 504

Query: 481 PNGWAPIQHMIVEGLARSGLKEARALAEDIASRWLRTNYVAYKQTGYMHEKYDVQKCGDF 540
           PNGWAPIQHMIVEGLARS L EA+ALAEDIA+RWLRTNYVAYK TG+MHEKYDVQKCG F
Sbjct: 505 PNGWAPIQHMIVEGLARSELPEAKALAEDIAARWLRTNYVAYKHTGFMHEKYDVQKCGGF 564

Query: 541 GGGGEYVPQTGFGWSNGVVLAFLEEFGWLEDQKIDCHIAS 581
           GGGGEYVPQTGFGWSNGVVLAFLEEFGW +DQKIDC++ S
Sbjct: 565 GGGGEYVPQTGFGWSNGVVLAFLEEFGWPKDQKIDCYLPS 601

BLAST of HG10009054 vs. NCBI nr
Match: XP_022145919.1 (probable trehalase [Momordica charantia])

HSP 1 Score: 1052.0 bits (2719), Expect = 1.9e-303
Identity = 493/580 (85.00%), Postives = 538/580 (92.76%), Query Frame = 0

Query: 1   MPLIPPAAMSLPEVLSPRLVDKGPVIPVTNLVKFLERLQVAALNSFGKLDFDLKYYVDLS 60
           +PL+   AM+LPEVL PR  D+GPV+PVT+LV+FLERLQ+ A NS GKLDFDLKYYVDLS
Sbjct: 22  LPLLLLPAMTLPEVLLPRAADRGPVVPVTDLVRFLERLQLVAFNSSGKLDFDLKYYVDLS 81

Query: 61  LKFDLNSTETAFDALERSSNGSVPVENLKAFIIDYFDSAGTDLVYSEPVDFFPQPVGFLP 120
           LK+DLNSTE AFDALER+ NGSVPVE LK FI +YF  AG DLVYSEP DF PQP GFLP
Sbjct: 82  LKYDLNSTELAFDALERAPNGSVPVEQLKTFIREYFGGAGNDLVYSEPADFVPQPTGFLP 141

Query: 121 KVENAEVRAWALDIHNFWKNLSRRVSDDLIHRPDTHTLLPLPEPVVIPGARFREVYYWDS 180
           KVENAEVRAWA DIHNFWKNLSRR SDDL+ RPD+HTLLPLP+P V+PG+RFRE+YYWDS
Sbjct: 142 KVENAEVRAWAFDIHNFWKNLSRRTSDDLLKRPDSHTLLPLPKPCVVPGSRFREIYYWDS 201

Query: 181 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 240
           YWIIRGLLASKMYDTAKGIVINLISMI++FGHVLNGARAYYTNRSQPPLLSSMVYDIYLR
Sbjct: 202 YWIIRGLLASKMYDTAKGIVINLISMIEDFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 261

Query: 241 TGDLEFVSNALPALIKEHKFWNSGFHSITVQNAPGGNGNHSLSRYYAMWNEPRPESSLLD 300
           T DLEFV  ALPALI+EHKFWNSGFHS+ V+NAPG  GNHSL RYYAMWNEPRPESSLLD
Sbjct: 262 TEDLEFVRTALPALIEEHKFWNSGFHSVAVRNAPG--GNHSLCRYYAMWNEPRPESSLLD 321

Query: 301 VKLASKLVNNNEKQHLYREIASAAESGWDFSTRWMRDPTDLSTLATTSILPVDLNVFILK 360
            KLASKLVNN+EKQHLYRE+A+AAESGWDFS+RWMRD TDLSTLAT+SILPVDLN F+LK
Sbjct: 322 EKLASKLVNNHEKQHLYRELATAAESGWDFSSRWMRDSTDLSTLATSSILPVDLNAFVLK 381

Query: 361 MELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHTW 420
           MELDISNLARA+GDYCTA+HF EAS+VRK+TIN+IFWNSEKGQWLDYWL+NGSYKGAHTW
Sbjct: 382 MELDISNLARAIGDYCTADHFLEASIVRKRTINAIFWNSEKGQWLDYWLENGSYKGAHTW 441

Query: 421 DARNQNQNVYASNFIPLWIESFYSDNKQMEKVLRSLRNSGLLCNAGIATSMINSGEQWDF 480
           DARNQNQ +YASNFIPLWIESFYSDN+Q++KVL+SLRNSGLLCNAGIATS+INSGEQWDF
Sbjct: 442 DARNQNQEIYASNFIPLWIESFYSDNRQLKKVLKSLRNSGLLCNAGIATSLINSGEQWDF 501

Query: 481 PNGWAPIQHMIVEGLARSGLKEARALAEDIASRWLRTNYVAYKQTGYMHEKYDVQKCGDF 540
           PNGWAPIQHMIVEGLARSGLKEARALAEDIA +WLRTNYVAYK TGYMHEKYDV+KCGDF
Sbjct: 502 PNGWAPIQHMIVEGLARSGLKEARALAEDIAMKWLRTNYVAYKNTGYMHEKYDVEKCGDF 561

Query: 541 GGGGEYVPQTGFGWSNGVVLAFLEEFGWLEDQKIDCHIAS 581
           GGGGEY+PQTGFGWSNGVVLAFLEEFGW  DQ IDC++ S
Sbjct: 562 GGGGEYIPQTGFGWSNGVVLAFLEEFGWPADQNIDCYLPS 599

BLAST of HG10009054 vs. ExPASy Swiss-Prot
Match: Q9SU50 (Trehalase OS=Arabidopsis thaliana OX=3702 GN=TRE1 PE=2 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 1.2e-194
Identity = 323/552 (58.51%), Postives = 417/552 (75.54%), Query Frame = 0

Query: 21  DKGPVIPVTNLVKFLERLQVAALNSFGKLDF-DLKYYVDLSLK--FDLNSTETAFDALER 80
           D GPV+  T LV FL+R+Q  AL S+ K    D K Y+DLSLK  + L++ E+AFD L  
Sbjct: 70  DSGPVVATTKLVTFLQRVQHTALRSYPKKQTPDPKSYIDLSLKRPYSLSTIESAFDDLTS 129

Query: 81  SSNGS-VPVENLKAFIIDYFDSAGTDLVYSEPVDFFPQPVGFLPKVENAEVRAWALDIHN 140
            S+   VPVE L+ F+ +YFD AG DL++ EPVDF   P GFL  VEN EVR WA ++H 
Sbjct: 130 ESHDQPVPVETLEKFVKEYFDGAGEDLLHHEPVDFVSDPSGFLSNVENEEVREWAREVHG 189

Query: 141 FWKNLSRRVSDDLIHRPDTHTLLPLPEPVVIPGARFREVYYWDSYWIIRGLLASKMYDTA 200
            W+NLS RVSD +    D HTLLPLPEPV+IPG+RFREVYYWDSYW+I+GL+ S+M+ TA
Sbjct: 190 LWRNLSCRVSDSVRESADRHTLLPLPEPVIIPGSRFREVYYWDSYWVIKGLMTSQMFTTA 249

Query: 201 KGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVSNALPALIK 260
           KG+V NL+S+++ +G+ LNGARAYYTNRSQPPLLSSMVY+IY  T D E V  A+P L+K
Sbjct: 250 KGLVTNLMSLVETYGYALNGARAYYTNRSQPPLLSSMVYEIYNVTKDEELVRKAIPLLLK 309

Query: 261 EHKFWNSGFHSITVQNAPGGNGNHSLSRYYAMWNEPRPESSLLDVKLASKLVNNNEKQHL 320
           E++FWNSG H + +++A G   +H LSRYYAMWN+PRPESS+ D + AS      EKQ  
Sbjct: 310 EYEFWNSGKHKVVIRDANG--YDHVLSRYYAMWNKPRPESSVFDEESASGFSTMLEKQRF 369

Query: 321 YREIASAAESGWDFSTRWMRDPTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDYC 380
           +R+IA+AAESG DFSTRWMRDP + +T+ATTS++PVDLNVF+LKMELDI+ + +  GD  
Sbjct: 370 HRDIATAAESGCDFSTRWMRDPPNFTTMATTSVVPVDLNVFLLKMELDIAFMMKVSGDQN 429

Query: 381 TAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHTWDARNQNQNVYASNFIP 440
            ++ F +AS  R+K   ++FWN + GQWLDYWL + S + + TW A NQN NV+ASNF P
Sbjct: 430 GSDRFVKASKAREKAFQTVFWNEKAGQWLDYWL-SSSGEESETWKAENQNTNVFASNFAP 489

Query: 441 LWIESFYSDNKQMEKVLRSLRNSGLLCNAGIATSMINSGEQWDFPNGWAPIQHMIVEGLA 500
           +WI S  SD   ++KV+ +L+NSGL+  AGI TS+ NSG+QWD PNGWAP Q MIV GL 
Sbjct: 490 IWINSINSDENLVKKVVTALKNSGLIAPAGILTSLTNSGQQWDSPNGWAPQQEMIVTGLG 549

Query: 501 RSGLKEARALAEDIASRWLRTNYVAYKQTGYMHEKYDVQKCGDFGGGGEYVPQTGFGWSN 560
           RS +KEA+ +AEDIA RW+++NY+ YK++G +HEK  V + G++GGGGEY+PQTGFGWSN
Sbjct: 550 RSSVKEAKEMAEDIARRWIKSNYLVYKKSGTIHEKLKVTELGEYGGGGEYMPQTGFGWSN 609

Query: 561 GVVLAFLEEFGW 569
           GV+LAFLEE+GW
Sbjct: 610 GVILAFLEEYGW 618

BLAST of HG10009054 vs. ExPASy Swiss-Prot
Match: Q9FWC1 (Probable trehalase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0521000 PE=2 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 2.1e-188
Identity = 322/570 (56.49%), Postives = 417/570 (73.16%), Query Frame = 0

Query: 15  LSPRLVDKGPVIPVTNLVKFLERLQVAALNSFGKLDFDLKYYVDLSLKFDLNSTETAFDA 74
           ++P     G  +    L+  L+R+Q  AL +FG  DFD K YVDL L  D  S   A  +
Sbjct: 1   MAPTAAVAGGGVEAEALLGLLQRVQSEALRAFGPNDFDPKLYVDLPLAADA-SAAAALAS 60

Query: 75  LERSSNGSVPVENLKAFIIDYFDSAGTDLV-YSEPVDFFPQPVGFLPKVENAEVRAWALD 134
           L R++     +E   A+I  YF  AG+DLV  ++P DF   P GFLP+VE AE RAWAL+
Sbjct: 61  LPRAAPSRGEME---AYISRYFALAGSDLVAAADPPDFERDPPGFLPRVERAEARAWALE 120

Query: 135 IHNFWKNLSRRVSDDLIHRPDTHTLLPLPEPVVIPGARFREVYYWDSYWIIRGLLASKMY 194
           +H  WK+L+RRV+  +  RPD HTLLPLP  VV+PG+RFREVYYWDSYW++RGLL SKMY
Sbjct: 121 VHALWKDLTRRVAPAVAARPDRHTLLPLPGRVVVPGSRFREVYYWDSYWVVRGLLVSKMY 180

Query: 195 DTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVSNALPA 254
           +TAK IV+NL+ +++++G VLNGAR+YYTNRSQPPLLSSMV DIY+ TGD+ FV    P+
Sbjct: 181 ETAKDIVLNLVYLVEKYGFVLNGARSYYTNRSQPPLLSSMVLDIYMATGDMAFVRRVFPS 240

Query: 255 LIKEHKFWNSGFHSITVQNAPGGNGNHSLSRYYAMWNEPRPESSLLDVKLASKLVNNNEK 314
           L+KEH FW S  H++ V +  G    H+LSRY AMWN+PRPES+ +D + ASKL +   K
Sbjct: 241 LLKEHSFWMSEVHNVAVMDNHG--RVHNLSRYQAMWNKPRPESATIDEEFASKL-STAAK 300

Query: 315 QHLYREIASAAESGWDFSTRWMRDPTDLSTLATTSILPVDLNVFILKMELDISNLARAVG 374
           +  Y ++AS AE+GWDFS+RWMRD TD++TL T+ I+PVDLN FILKME DI+  A+ +G
Sbjct: 301 EKFYHQVASTAETGWDFSSRWMRDSTDMTTLTTSCIIPVDLNTFILKMEQDIAFFAKLIG 360

Query: 375 DYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWL-DNGSYKGAHTWDARNQNQNVYAS 434
           +  T+E F EAS  R   I+S+ WN++  QWLDYWL  +G+ +G + W + +QN+ ++AS
Sbjct: 361 ESTTSEIFSEASKARHNAIDSVLWNADMEQWLDYWLPTDGNCQGVYQWKSISQNRAIFAS 420

Query: 435 NFIPLWIESFYS------DNKQMEKVLRSLRNSGLLCNAGIATSMINSGEQWDFPNGWAP 494
           NF+PLW+ + +S      D  +  +V+RSL+ SGLL  AGIATS+ N+G+QWDFPNGWAP
Sbjct: 421 NFVPLWLNAQHSGLEQFVDEAKSVRVMRSLQKSGLLQPAGIATSLSNTGQQWDFPNGWAP 480

Query: 495 IQHMIVEGLARSGLKEARALAEDIASRWLRTNYVAYKQTGYMHEKYDVQKCGDFGGGGEY 554
           +QH+IVEGL RSG  EAR LAEDIA+RW+RTNY AYK TG MHEKYDV  CG  GGGGEY
Sbjct: 481 LQHLIVEGLLRSGSGEARELAEDIATRWVRTNYDAYKATGAMHEKYDVVTCGKSGGGGEY 540

Query: 555 VPQTGFGWSNGVVLAFLEEFGWLEDQKIDC 577
            PQTGFGWSNGV+L+FL+EFGW +D+KIDC
Sbjct: 541 KPQTGFGWSNGVILSFLDEFGWPQDKKIDC 563

BLAST of HG10009054 vs. ExPASy Swiss-Prot
Match: O43280 (Trehalase OS=Homo sapiens OX=9606 GN=TREH PE=1 SV=2)

HSP 1 Score: 390.2 bits (1001), Expect = 4.1e-107
Identity = 205/525 (39.05%), Postives = 303/525 (57.71%), Query Frame = 0

Query: 48  KLDFDLKYYVDLSLKFDLNSTETAFDALERSSNGSVPVENLKAFIIDYFDSAGTDLVYSE 107
           KL  D K +VD+ L          F  L R  N S+P E L+AF+ ++F + G +L    
Sbjct: 46  KLYQDDKQFVDMPLSIAPEQVLQTFTELSRDHNHSIPREQLQAFVHEHFQAKGQELQPWT 105

Query: 108 PVDFFPQPVGFLPKVENAEVRAWALDIHNFWKNLSRRVSDDLIHRPDTHTLLPLPEPVVI 167
           P D+   P  FL K+ +A++RAWA  +H  WK L +++  +++  P+  +L+    P ++
Sbjct: 106 PADWKDSP-QFLQKISDAKLRAWAGQLHQLWKKLGKKMKPEVLSHPERFSLIYSEHPFIV 165

Query: 168 PGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQP 227
           PG RF E YYWDSYW++ GLL S+M +T KG++ N + ++  +GHV NG R YY  RSQP
Sbjct: 166 PGGRFVEFYYWDSYWVMEGLLLSEMAETVKGMLQNFLDLVKTYGHVPNGGRVYYLQRSQP 225

Query: 228 PLLSSMVYDIYL-RTGDLEFVSNALPALIKEHKFWNSGFHSITVQNAPGGNGNHSLSRYY 287
           PLL+ M+ D YL  T D  F+   +  L  E  FW     + TV  +  G  N+ L+RYY
Sbjct: 226 PLLTLMM-DCYLTHTNDTAFLQENIETLALELDFWTK---NRTVSVSLEGK-NYLLNRYY 285

Query: 288 AMWNEPRPESSLLDVKLASKLVNNNEKQHLYREIASAAESGWDFSTRWM---RDPTDLST 347
             +  PRPES   DV+LA  L    +++ L+ E+ + AESGWDFS+RW+    +P  LS 
Sbjct: 286 VPYGGPRPESYSKDVELADTL-PEGDREALWAELKAGAESGWDFSSRWLIGGPNPNSLSG 345

Query: 348 LATTSILPVDLNVFILKMELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQ 407
           + T+ ++PVDLN F+ + E  +SN    +G+   A  +      R   +N++ W+ + G 
Sbjct: 346 IRTSKLVPVDLNAFLCQAEELMSNFYSRLGNDSQATKYRILRSQRLAALNTVLWDEQTGA 405

Query: 408 WLDYWLDNGSYKGAHTWDARNQNQNVYASNFIPLWIESFYSDNKQMEKVLRSLRNSGLLC 467
           W DY L+            + +N+  Y SN  PLW   F SD    +K L+ L ++ +L 
Sbjct: 406 WFDYDLEK-----------KKKNREFYPSNLTPLWAGCF-SDPGVADKALKYLEDNRILT 465

Query: 468 -NAGIATSMINSGEQWDFPNGWAPIQHMIVEGLARSGLKEARALAEDIASRWLRTNYVAY 527
              GI TS+  +G+QWDFPN WAP+Q +++ GLA++ L+ A+ +A  +A  W+RTN+  Y
Sbjct: 466 YQYGIPTSLQKTGQQWDFPNAWAPLQDLVIRGLAKAPLRRAQEVAFQLAQNWIRTNFDVY 525

Query: 528 KQTGYMHEKYDVQKCGDFGGGGEYVPQTGFGWSNGVVLAFLEEFG 568
            Q   M+EKYDV   G  GGGGEY  Q GFGW+NGVVL  L+ +G
Sbjct: 526 SQKSAMYEKYDVSNGGQPGGGGEYEVQEGFGWTNGVVLMLLDRYG 551

BLAST of HG10009054 vs. ExPASy Swiss-Prot
Match: Q9JLT2 (Trehalase OS=Mus musculus OX=10090 GN=Treh PE=1 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.2e-103
Identity = 198/539 (36.73%), Postives = 298/539 (55.29%), Query Frame = 0

Query: 33  KFLERLQVAALNSFGKLDFDLKYYVDLSLKFDLNSTETAFDALERSSNGSVPVENLKAFI 92
           + L ++Q+A L        D K +VD+SL    +     F  L    N S+P E L+ F+
Sbjct: 34  ELLHQVQMAQLYQ------DDKQFVDMSLATSPDEVLQKFSELATVHNHSIPKEQLQEFV 93

Query: 93  IDYFDSAGTDLVYSEPVDFFPQPVGFLPKVENAEVRAWALDIHNFWKNLSRRVSDDLIHR 152
             +F   G +L    P D+   P  FL K+ +A +R WA ++H  WK L +++  +++  
Sbjct: 94  QSHFQPVGQELQSWTPEDWKDSP-QFLQKISDANLRVWAEELHKIWKKLGKKMKAEVLSY 153

Query: 153 PDTHTLLPLPEPVVIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGH 212
           P+  +L+    P ++PG RF E YYWDSYW++ GLL S+M  T KG++ N + ++  +GH
Sbjct: 154 PERSSLIYSKHPFIVPGGRFVEFYYWDSYWVMEGLLLSEMASTVKGMLQNFLDLVKTYGH 213

Query: 213 VLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVSNALPALIKEHKFWNSGFHSITVQN 272
           + NG R YY  RSQPPLL+ M+      T D+ F+   +  L  E  FW     + TV  
Sbjct: 214 IPNGGRIYYLQRSQPPLLTLMMDRYVAHTKDVAFLQENIGTLASELDFWTV---NRTVSV 273

Query: 273 APGGNGNHSLSRYYAMWNEPRPESSLLDVKLASKLVNNNEKQHLYREIASAAESGWDFST 332
             GG  ++ L+RYY  +  PRPES   D +LA+  V   +++ L+ E+ + AESGWDFS+
Sbjct: 274 VSGGQ-SYVLNRYYVPYGGPRPESYRKDAELANS-VPEGDRETLWAELKAGAESGWDFSS 333

Query: 333 RWM---RDPTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDYCTAEHFFEASLVRK 392
           RW+    DP  LS++ T+ ++P DLN F+ + E  +SN    +G+   A  +      R 
Sbjct: 334 RWLVGGPDPDLLSSIRTSKMVPADLNAFLCQAEELMSNFYSRLGNDTEATKYRNLRAQRL 393

Query: 393 KTINSIFWNSEKGQWLDYWLDNGSYKGAHTWDARNQNQNVYASNFIPLWIESFYSDNKQM 452
             + ++ W+ +KG W DY L+ G            +N   Y SN  PLW   F SD    
Sbjct: 394 AAMEAVLWDEQKGAWFDYDLEKG-----------KKNLEFYPSNLSPLWAGCF-SDPSVA 453

Query: 453 EKVLRSLRNSGLLC-NAGIATSMINSGEQWDFPNGWAPIQHMIVEGLARSGLKEARALAE 512
           +K L+ L +S +L    GI TS+ N+G+QWDFPN WAP+Q +++ GLA+S     + +A 
Sbjct: 454 DKALKYLEDSKILTYQYGIPTSLRNTGQQWDFPNAWAPLQDLVIRGLAKSASPRTQEVAF 513

Query: 513 DIASRWLRTNYVAYKQTGYMHEKYDVQKCGDFGGGGEYVPQTGFGWSNGVVLAFLEEFG 568
            +A  W++TN+  Y Q   M EKYD+   G  GGGGEY  Q GFGW+NG+ L  L+ +G
Sbjct: 514 QLAQNWIKTNFKVYSQKSAMFEKYDISNGGHPGGGGEYEVQEGFGWTNGLALMLLDRYG 548

BLAST of HG10009054 vs. ExPASy Swiss-Prot
Match: P19813 (Trehalase OS=Oryctolagus cuniculus OX=9986 GN=TREH PE=1 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.2e-103
Identity = 194/520 (37.31%), Postives = 299/520 (57.50%), Query Frame = 0

Query: 52  DLKYYVDLSLKFDLNSTETAFDALERSSNGSVPVENLKAFIIDYFDSAGTDLVYSEPVDF 111
           D K +VD+ L    +    +F  L  + N +VP E L+ F+ ++F + G +L    P D+
Sbjct: 50  DDKQFVDMPLSTAPDQVLQSFAELAATYNNTVPREQLEKFVQEHFQAVGQELESWTPGDW 109

Query: 112 FPQPVGFLPKVENAEVRAWALDIHNFWKNLSRRVSDDLIHRPDTHTLLPLPEPVVIPGAR 171
              P  FL K+ + ++RAWA  +H  WK L +++  +++ +P+  +L+    P ++PG R
Sbjct: 110 KESP-QFLQKISDPKLRAWAEQLHLLWKKLGKKIKPEVLSQPERFSLIYSQHPFIVPGGR 169

Query: 172 FREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLS 231
           F E YYWDSYW++ GLL S+M +T KG++ N + ++  +GH+ NG R YY  RSQPPLL+
Sbjct: 170 FVEFYYWDSYWVMEGLLLSEMAETVKGMLQNFLDLVTAYGHIPNGGRVYYLQRSQPPLLT 229

Query: 232 SMVYDIYLRTGDLEFVSNALPALIKEHKFWNSGFHSITVQNAPGGNGNHSLSRYYAMWNE 291
            M+      TGDL F+   +  L  E  FW     + T+  + GGN +H+L+RY+  +  
Sbjct: 230 LMMDRYVAHTGDLAFLRENIETLALELDFW---AENRTISVSSGGN-SHTLNRYHVPYGG 289

Query: 292 PRPESSLLDVKLASKLVNNNEKQHLYREIASAAESGWDFSTRWM---RDPTDLSTLATTS 351
           PRPES   D +LA  L   +  + L+ E+ + AESGWDFS+RW+    +P  L ++ T+ 
Sbjct: 290 PRPESYSKDTELAHTLPEGS-WETLWAELKAGAESGWDFSSRWLVGSPNPDSLGSIRTSK 349

Query: 352 ILPVDLNVFILKMELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYW 411
           ++PVDLN F+ + E  +S     +G+   A  +      R   + ++ W+ +KG W DY 
Sbjct: 350 LVPVDLNAFLCQAEELLSGFYSRLGNESQATKYRNLRAQRIAALTALLWDEDKGAWFDYD 409

Query: 412 LDNGSYKGAHTWDARNQNQNVYASNFIPLWIESFYSDNKQMEKVLRSLRNSGLLCNA-GI 471
           L+N           + +N   Y SN  PLW   F SD    +K L+ L++S +L +  GI
Sbjct: 410 LEN-----------QKKNHEFYPSNLTPLWAGCF-SDPAIADKALQYLQDSQILNHRHGI 469

Query: 472 ATSMINSGEQWDFPNGWAPIQHMIVEGLARSGLKEARALAEDIASRWLRTNYVAYKQTGY 531
            TS+ N+G+QWDFPN WAP+Q +++ GLA+S     + +A  +A  W+RTN+  Y Q   
Sbjct: 470 PTSLQNTGQQWDFPNAWAPLQDLVIRGLAKSPSARTQEVAFQLAQNWIRTNFDVYSQRSA 529

Query: 532 MHEKYDVQKCGDFGGGGEYVPQTGFGWSNGVVLAFLEEFG 568
           M+EKYD+      GGGGEY  Q GFGW+NGV L  L+ +G
Sbjct: 530 MYEKYDISNAQP-GGGGEYEVQEGFGWTNGVALMLLDRYG 550

BLAST of HG10009054 vs. ExPASy TrEMBL
Match: A0A5A7TH01 (Trehalase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001860 PE=3 SV=1)

HSP 1 Score: 1090.9 bits (2820), Expect = 0.0e+00
Identity = 522/580 (90.00%), Postives = 549/580 (94.66%), Query Frame = 0

Query: 1   MPLIPPAAMSLPEVLSPRLVDKGPVIPVTNLVKFLERLQVAALNSFGKLDFDLKYYVDLS 60
           MPL+ PAA SLPEV SPRL DKGPVIPV NLVKFLERLQV ALNSFGKLDFDLKYYVDLS
Sbjct: 1   MPLLRPAAASLPEVFSPRLADKGPVIPVPNLVKFLERLQVVALNSFGKLDFDLKYYVDLS 60

Query: 61  LKFDLNSTETAFDALERSSNGSVPVENLKAFIIDYFDSAGTDLVYSEPVDFFPQPVGFLP 120
           LKFDLNST+ AFDALERSSNGSV VENL+ FI +YF SAGTDLVYS PVDF PQP GFLP
Sbjct: 61  LKFDLNSTQIAFDALERSSNGSVSVENLRTFISNYFHSAGTDLVYSNPVDFNPQPDGFLP 120

Query: 121 KVENAEVRAWALDIHNFWKNLSRRVSDDLIHRPDTHTLLPLPEPVVIPGARFREVYYWDS 180
           KVEN EVR WALDIHNFWKNLSRRVSDDLIH PDTHTLLPLPEPVV+PG+RFRE+YYWDS
Sbjct: 121 KVENVEVRTWALDIHNFWKNLSRRVSDDLIHHPDTHTLLPLPEPVVVPGSRFREIYYWDS 180

Query: 181 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 240
           YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGAR+YYTNRSQPPLLSSMVYDIYLR
Sbjct: 181 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARSYYTNRSQPPLLSSMVYDIYLR 240

Query: 241 TGDLEFVSNALPALIKEHKFWNSGFHSITVQNAPGGNGNHSLSRYYAMWNEPRPESSLLD 300
           TGDLEFV N+LPALIKEHKFWNSGFHS+TVQN   GNGNHSLSRYYAMWNEPRPESSL+D
Sbjct: 241 TGDLEFVRNSLPALIKEHKFWNSGFHSVTVQN---GNGNHSLSRYYAMWNEPRPESSLVD 300

Query: 301 VKLASKLVNNNEKQHLYREIASAAESGWDFSTRWMRDPTDLSTLATTSILPVDLNVFILK 360
            KLASK  NN EK+HLYREIASAAESGWDFS+RWMRD TDLSTLATTSILPVDLNVFILK
Sbjct: 301 EKLASKFANNYEKKHLYREIASAAESGWDFSSRWMRDSTDLSTLATTSILPVDLNVFILK 360

Query: 361 MELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHTW 420
           MELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAH+W
Sbjct: 361 MELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHSW 420

Query: 421 DARNQNQNVYASNFIPLWIESFYSDNKQMEKVLRSLRNSGLLCNAGIATSMINSGEQWDF 480
           +ARNQNQN+YASNFIPLWIESFYSD++QM+KVL+SLRNSGLLCNAGIATS+INSGEQWDF
Sbjct: 421 NARNQNQNIYASNFIPLWIESFYSDSRQMKKVLKSLRNSGLLCNAGIATSVINSGEQWDF 480

Query: 481 PNGWAPIQHMIVEGLARSGLKEARALAEDIASRWLRTNYVAYKQTGYMHEKYDVQKCGDF 540
           PNGWAPIQHMIVEGLARS L EA+ALAEDIA+RWLRTNYVAYK TG+MHEKYDVQKCGDF
Sbjct: 481 PNGWAPIQHMIVEGLARSELPEAKALAEDIAARWLRTNYVAYKHTGFMHEKYDVQKCGDF 540

Query: 541 GGGGEYVPQTGFGWSNGVVLAFLEEFGWLEDQKIDCHIAS 581
           GGGGEYVPQTGFGWSNGVVLAFLEEFGW +DQKIDC++ S
Sbjct: 541 GGGGEYVPQTGFGWSNGVVLAFLEEFGWPKDQKIDCYLPS 577

BLAST of HG10009054 vs. ExPASy TrEMBL
Match: A0A1S3ATK4 (Trehalase OS=Cucumis melo OX=3656 GN=LOC103482823 PE=3 SV=1)

HSP 1 Score: 1090.9 bits (2820), Expect = 0.0e+00
Identity = 522/580 (90.00%), Postives = 549/580 (94.66%), Query Frame = 0

Query: 1   MPLIPPAAMSLPEVLSPRLVDKGPVIPVTNLVKFLERLQVAALNSFGKLDFDLKYYVDLS 60
           MPL+ PAA SLPEV SPRL DKGPVIPV NLVKFLERLQV ALNSFGKLDFDLKYYVDLS
Sbjct: 25  MPLLRPAAASLPEVFSPRLADKGPVIPVPNLVKFLERLQVVALNSFGKLDFDLKYYVDLS 84

Query: 61  LKFDLNSTETAFDALERSSNGSVPVENLKAFIIDYFDSAGTDLVYSEPVDFFPQPVGFLP 120
           LKFDLNST+ AFDALERSSNGSV VENL+ FI +YF SAGTDLVYS PVDF PQP GFLP
Sbjct: 85  LKFDLNSTQIAFDALERSSNGSVSVENLRTFISNYFHSAGTDLVYSNPVDFNPQPDGFLP 144

Query: 121 KVENAEVRAWALDIHNFWKNLSRRVSDDLIHRPDTHTLLPLPEPVVIPGARFREVYYWDS 180
           KVEN EVR WALDIHNFWKNLSRRVSDDLIH PDTHTLLPLPEPVV+PG+RFRE+YYWDS
Sbjct: 145 KVENVEVRTWALDIHNFWKNLSRRVSDDLIHHPDTHTLLPLPEPVVVPGSRFREIYYWDS 204

Query: 181 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 240
           YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGAR+YYTNRSQPPLLSSMVYDIYLR
Sbjct: 205 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARSYYTNRSQPPLLSSMVYDIYLR 264

Query: 241 TGDLEFVSNALPALIKEHKFWNSGFHSITVQNAPGGNGNHSLSRYYAMWNEPRPESSLLD 300
           TGDLEFV N+LPALIKEHKFWNSGFHS+TVQN   GNGNHSLSRYYAMWNEPRPESSL+D
Sbjct: 265 TGDLEFVRNSLPALIKEHKFWNSGFHSVTVQN---GNGNHSLSRYYAMWNEPRPESSLVD 324

Query: 301 VKLASKLVNNNEKQHLYREIASAAESGWDFSTRWMRDPTDLSTLATTSILPVDLNVFILK 360
            KLASK  NN EK+HLYREIASAAESGWDFS+RWMRD TDLSTLATTSILPVDLNVFILK
Sbjct: 325 EKLASKFANNYEKKHLYREIASAAESGWDFSSRWMRDSTDLSTLATTSILPVDLNVFILK 384

Query: 361 MELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHTW 420
           MELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAH+W
Sbjct: 385 MELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHSW 444

Query: 421 DARNQNQNVYASNFIPLWIESFYSDNKQMEKVLRSLRNSGLLCNAGIATSMINSGEQWDF 480
           +ARNQNQN+YASNFIPLWIESFYSD++QM+KVL+SLRNSGLLCNAGIATS+INSGEQWDF
Sbjct: 445 NARNQNQNIYASNFIPLWIESFYSDSRQMKKVLKSLRNSGLLCNAGIATSVINSGEQWDF 504

Query: 481 PNGWAPIQHMIVEGLARSGLKEARALAEDIASRWLRTNYVAYKQTGYMHEKYDVQKCGDF 540
           PNGWAPIQHMIVEGLARS L EA+ALAEDIA+RWLRTNYVAYK TG+MHEKYDVQKCGDF
Sbjct: 505 PNGWAPIQHMIVEGLARSELPEAKALAEDIAARWLRTNYVAYKHTGFMHEKYDVQKCGDF 564

Query: 541 GGGGEYVPQTGFGWSNGVVLAFLEEFGWLEDQKIDCHIAS 581
           GGGGEYVPQTGFGWSNGVVLAFLEEFGW +DQKIDC++ S
Sbjct: 565 GGGGEYVPQTGFGWSNGVVLAFLEEFGWPKDQKIDCYLPS 601

BLAST of HG10009054 vs. ExPASy TrEMBL
Match: A0A0A0KMF2 (Trehalase OS=Cucumis sativus OX=3659 GN=Csa_5G149880 PE=3 SV=1)

HSP 1 Score: 1089.3 bits (2816), Expect = 0.0e+00
Identity = 521/580 (89.83%), Postives = 547/580 (94.31%), Query Frame = 0

Query: 1   MPLIPPAAMSLPEVLSPRLVDKGPVIPVTNLVKFLERLQVAALNSFGKLDFDLKYYVDLS 60
           MPL+ PAA SLPEV SPRL DKGPVIPV NLVKFLER+QV ALNSFGKLDFDLKYYVDLS
Sbjct: 25  MPLLRPAAASLPEVFSPRLADKGPVIPVPNLVKFLERIQVVALNSFGKLDFDLKYYVDLS 84

Query: 61  LKFDLNSTETAFDALERSSNGSVPVENLKAFIIDYFDSAGTDLVYSEPVDFFPQPVGFLP 120
           LKFDLNST+ AFDALERSSNGSV VENL+ FI +YFDSAGTDLVYS P DF P P GFLP
Sbjct: 85  LKFDLNSTQIAFDALERSSNGSVSVENLRTFISNYFDSAGTDLVYSNPADFDPHPDGFLP 144

Query: 121 KVENAEVRAWALDIHNFWKNLSRRVSDDLIHRPDTHTLLPLPEPVVIPGARFREVYYWDS 180
           KVEN EVRAWA DIHNFWKNLSRRVSDDLIH PDTHTLLPLPEPVV+PG+RFRE+YYWDS
Sbjct: 145 KVENVEVRAWAFDIHNFWKNLSRRVSDDLIHHPDTHTLLPLPEPVVVPGSRFREIYYWDS 204

Query: 181 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 240
           YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGAR+YYTNRSQPPLLSSMVYDIYLR
Sbjct: 205 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARSYYTNRSQPPLLSSMVYDIYLR 264

Query: 241 TGDLEFVSNALPALIKEHKFWNSGFHSITVQNAPGGNGNHSLSRYYAMWNEPRPESSLLD 300
           TGDLEFV N+LPALIKEHKFWNSGFHSITVQN   GNGNHSLSRYYAMWNEPRPESSL+D
Sbjct: 265 TGDLEFVRNSLPALIKEHKFWNSGFHSITVQN---GNGNHSLSRYYAMWNEPRPESSLVD 324

Query: 301 VKLASKLVNNNEKQHLYREIASAAESGWDFSTRWMRDPTDLSTLATTSILPVDLNVFILK 360
            K+ASK VNN EK+HLYREIASAAESGWDFS+RWMRD TDLSTLATTSILPVDLNVFILK
Sbjct: 325 EKVASKFVNNYEKKHLYREIASAAESGWDFSSRWMRDSTDLSTLATTSILPVDLNVFILK 384

Query: 361 MELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHTW 420
           MELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAH+W
Sbjct: 385 MELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHSW 444

Query: 421 DARNQNQNVYASNFIPLWIESFYSDNKQMEKVLRSLRNSGLLCNAGIATSMINSGEQWDF 480
           D RNQNQNVYASNFIPLW+ESFYSD++QM+KVL+SLRNSGLLCNAGIATSMINSGEQWDF
Sbjct: 445 DVRNQNQNVYASNFIPLWVESFYSDSRQMKKVLKSLRNSGLLCNAGIATSMINSGEQWDF 504

Query: 481 PNGWAPIQHMIVEGLARSGLKEARALAEDIASRWLRTNYVAYKQTGYMHEKYDVQKCGDF 540
           PNGWAPIQHMIVEGLARS L EA+ALAEDIA+RWLRTNYVAYK TG+MHEKYDVQKCG F
Sbjct: 505 PNGWAPIQHMIVEGLARSELPEAKALAEDIAARWLRTNYVAYKHTGFMHEKYDVQKCGGF 564

Query: 541 GGGGEYVPQTGFGWSNGVVLAFLEEFGWLEDQKIDCHIAS 581
           GGGGEYVPQTGFGWSNGVVLAFLEEFGW +DQKIDC++ S
Sbjct: 565 GGGGEYVPQTGFGWSNGVVLAFLEEFGWPKDQKIDCYLPS 601

BLAST of HG10009054 vs. ExPASy TrEMBL
Match: A0A6J1CWN7 (Trehalase OS=Momordica charantia OX=3673 GN=LOC111015267 PE=3 SV=1)

HSP 1 Score: 1052.0 bits (2719), Expect = 9.3e-304
Identity = 493/580 (85.00%), Postives = 538/580 (92.76%), Query Frame = 0

Query: 1   MPLIPPAAMSLPEVLSPRLVDKGPVIPVTNLVKFLERLQVAALNSFGKLDFDLKYYVDLS 60
           +PL+   AM+LPEVL PR  D+GPV+PVT+LV+FLERLQ+ A NS GKLDFDLKYYVDLS
Sbjct: 22  LPLLLLPAMTLPEVLLPRAADRGPVVPVTDLVRFLERLQLVAFNSSGKLDFDLKYYVDLS 81

Query: 61  LKFDLNSTETAFDALERSSNGSVPVENLKAFIIDYFDSAGTDLVYSEPVDFFPQPVGFLP 120
           LK+DLNSTE AFDALER+ NGSVPVE LK FI +YF  AG DLVYSEP DF PQP GFLP
Sbjct: 82  LKYDLNSTELAFDALERAPNGSVPVEQLKTFIREYFGGAGNDLVYSEPADFVPQPTGFLP 141

Query: 121 KVENAEVRAWALDIHNFWKNLSRRVSDDLIHRPDTHTLLPLPEPVVIPGARFREVYYWDS 180
           KVENAEVRAWA DIHNFWKNLSRR SDDL+ RPD+HTLLPLP+P V+PG+RFRE+YYWDS
Sbjct: 142 KVENAEVRAWAFDIHNFWKNLSRRTSDDLLKRPDSHTLLPLPKPCVVPGSRFREIYYWDS 201

Query: 181 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 240
           YWIIRGLLASKMYDTAKGIVINLISMI++FGHVLNGARAYYTNRSQPPLLSSMVYDIYLR
Sbjct: 202 YWIIRGLLASKMYDTAKGIVINLISMIEDFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 261

Query: 241 TGDLEFVSNALPALIKEHKFWNSGFHSITVQNAPGGNGNHSLSRYYAMWNEPRPESSLLD 300
           T DLEFV  ALPALI+EHKFWNSGFHS+ V+NAPG  GNHSL RYYAMWNEPRPESSLLD
Sbjct: 262 TEDLEFVRTALPALIEEHKFWNSGFHSVAVRNAPG--GNHSLCRYYAMWNEPRPESSLLD 321

Query: 301 VKLASKLVNNNEKQHLYREIASAAESGWDFSTRWMRDPTDLSTLATTSILPVDLNVFILK 360
            KLASKLVNN+EKQHLYRE+A+AAESGWDFS+RWMRD TDLSTLAT+SILPVDLN F+LK
Sbjct: 322 EKLASKLVNNHEKQHLYRELATAAESGWDFSSRWMRDSTDLSTLATSSILPVDLNAFVLK 381

Query: 361 MELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHTW 420
           MELDISNLARA+GDYCTA+HF EAS+VRK+TIN+IFWNSEKGQWLDYWL+NGSYKGAHTW
Sbjct: 382 MELDISNLARAIGDYCTADHFLEASIVRKRTINAIFWNSEKGQWLDYWLENGSYKGAHTW 441

Query: 421 DARNQNQNVYASNFIPLWIESFYSDNKQMEKVLRSLRNSGLLCNAGIATSMINSGEQWDF 480
           DARNQNQ +YASNFIPLWIESFYSDN+Q++KVL+SLRNSGLLCNAGIATS+INSGEQWDF
Sbjct: 442 DARNQNQEIYASNFIPLWIESFYSDNRQLKKVLKSLRNSGLLCNAGIATSLINSGEQWDF 501

Query: 481 PNGWAPIQHMIVEGLARSGLKEARALAEDIASRWLRTNYVAYKQTGYMHEKYDVQKCGDF 540
           PNGWAPIQHMIVEGLARSGLKEARALAEDIA +WLRTNYVAYK TGYMHEKYDV+KCGDF
Sbjct: 502 PNGWAPIQHMIVEGLARSGLKEARALAEDIAMKWLRTNYVAYKNTGYMHEKYDVEKCGDF 561

Query: 541 GGGGEYVPQTGFGWSNGVVLAFLEEFGWLEDQKIDCHIAS 581
           GGGGEY+PQTGFGWSNGVVLAFLEEFGW  DQ IDC++ S
Sbjct: 562 GGGGEYIPQTGFGWSNGVVLAFLEEFGWPADQNIDCYLPS 599

BLAST of HG10009054 vs. ExPASy TrEMBL
Match: A0A6J1K2W1 (Trehalase OS=Cucurbita maxima OX=3661 GN=LOC111491217 PE=3 SV=1)

HSP 1 Score: 1049.3 bits (2712), Expect = 6.0e-303
Identity = 507/577 (87.87%), Postives = 535/577 (92.72%), Query Frame = 0

Query: 1   MPLIPPAA-MSLPEVLSPRLVDKGPVIPVTNLVKFLERLQVAALNSFGKLDFDLKYYVDL 60
           +PL+PPAA M L EV SPRL  +GPVIPV+NLVKFLERLQV ALNSFGKLDFDLKYYVDL
Sbjct: 25  VPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKFLERLQVVALNSFGKLDFDLKYYVDL 84

Query: 61  SLKFDLNSTETAFDALERSSNGSVPVENLKAFIIDYFDSAGTDLVYSEPVDFFPQPVGFL 120
           SLKFDLNSTE AFDAL RSSNGSVPVE+LK FI +YFDSAGTD+VYS+PVDF P+P GFL
Sbjct: 85  SLKFDLNSTERAFDALSRSSNGSVPVEDLKTFIGEYFDSAGTDMVYSDPVDFVPRPQGFL 144

Query: 121 PKVENAEVRAWALDIHNFWKNLSRRVSDDLIHRPDTHTLLPLPEPVVIPGARFREVYYWD 180
           PKVENA VRAWAL+IHN+WKNLSR+VSDD+IHRPD HTLLPLP P VIPGARFREVYYWD
Sbjct: 145 PKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCVIPGARFREVYYWD 204

Query: 181 SYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYL 240
           SYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYL
Sbjct: 205 SYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYL 264

Query: 241 RTGDLEFVSNALPALIKEHKFWNSGFHSITVQNAPGGNGNHSLSRYYAMWNEPRPESSLL 300
           RTGDLEFV NALPALIKEH FWNSGFHS+TV+ APG  GNHSLSRYYAMWNEPRPESSLL
Sbjct: 265 RTGDLEFVRNALPALIKEHTFWNSGFHSVTVRKAPG--GNHSLSRYYAMWNEPRPESSLL 324

Query: 301 DVKLASKLVNNNEKQHLYREIASAAESGWDFSTRWMRDPTDLSTLATTSILPVDLNVFIL 360
           D KLASK VNN +KQ LY E+AS AESGWDFS+RWMRD TDLSTLATTSILPVDLNVFIL
Sbjct: 325 DEKLASKFVNNYDKQRLYSELASTAESGWDFSSRWMRDHTDLSTLATTSILPVDLNVFIL 384

Query: 361 KMELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHT 420
           KMELDISNLARAVGD+C A  F EASLVRKKTINSIFWNSEKGQWLDYWLDNGS+KG HT
Sbjct: 385 KMELDISNLARAVGDFCLAYKFLEASLVRKKTINSIFWNSEKGQWLDYWLDNGSHKGVHT 444

Query: 421 WDARNQNQNVYASNFIPLWIESFYSDNKQMEKVLRSLRNSGLLCNAGIATSMINSGEQWD 480
           WDA+NQN+N+YASNFIPLWIESFYSD+ QM+KVL+SLRNSGLLCNAGIATS INSGEQWD
Sbjct: 445 WDAQNQNKNIYASNFIPLWIESFYSDSIQMKKVLKSLRNSGLLCNAGIATSTINSGEQWD 504

Query: 481 FPNGWAPIQHMIVEGLARSGLKEARALAEDIASRWLRTNYVAYKQTGYMHEKYDVQKCGD 540
           FPNGWAPIQHMIVEGLARS LKEAR LAED+A RWLRTNYVAYK+TGYMHEKYDVQKCGD
Sbjct: 505 FPNGWAPIQHMIVEGLARSELKEARVLAEDMAVRWLRTNYVAYKKTGYMHEKYDVQKCGD 564

Query: 541 FGGGGEYVPQTGFGWSNGVVLAFLEEFGWLEDQKIDC 577
           FG GGEYVPQTGFGWSNGVVLAFLEEFGW EDQ IDC
Sbjct: 565 FGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 599

BLAST of HG10009054 vs. TAIR 10
Match: AT4G24040.1 (trehalase 1 )

HSP 1 Score: 681.0 bits (1756), Expect = 8.3e-196
Identity = 323/552 (58.51%), Postives = 417/552 (75.54%), Query Frame = 0

Query: 21  DKGPVIPVTNLVKFLERLQVAALNSFGKLDF-DLKYYVDLSLK--FDLNSTETAFDALER 80
           D GPV+  T LV FL+R+Q  AL S+ K    D K Y+DLSLK  + L++ E+AFD L  
Sbjct: 70  DSGPVVATTKLVTFLQRVQHTALRSYPKKQTPDPKSYIDLSLKRPYSLSTIESAFDDLTS 129

Query: 81  SSNGS-VPVENLKAFIIDYFDSAGTDLVYSEPVDFFPQPVGFLPKVENAEVRAWALDIHN 140
            S+   VPVE L+ F+ +YFD AG DL++ EPVDF   P GFL  VEN EVR WA ++H 
Sbjct: 130 ESHDQPVPVETLEKFVKEYFDGAGEDLLHHEPVDFVSDPSGFLSNVENEEVREWAREVHG 189

Query: 141 FWKNLSRRVSDDLIHRPDTHTLLPLPEPVVIPGARFREVYYWDSYWIIRGLLASKMYDTA 200
            W+NLS RVSD +    D HTLLPLPEPV+IPG+RFREVYYWDSYW+I+GL+ S+M+ TA
Sbjct: 190 LWRNLSCRVSDSVRESADRHTLLPLPEPVIIPGSRFREVYYWDSYWVIKGLMTSQMFTTA 249

Query: 201 KGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVSNALPALIK 260
           KG+V NL+S+++ +G+ LNGARAYYTNRSQPPLLSSMVY+IY  T D E V  A+P L+K
Sbjct: 250 KGLVTNLMSLVETYGYALNGARAYYTNRSQPPLLSSMVYEIYNVTKDEELVRKAIPLLLK 309

Query: 261 EHKFWNSGFHSITVQNAPGGNGNHSLSRYYAMWNEPRPESSLLDVKLASKLVNNNEKQHL 320
           E++FWNSG H + +++A G   +H LSRYYAMWN+PRPESS+ D + AS      EKQ  
Sbjct: 310 EYEFWNSGKHKVVIRDANG--YDHVLSRYYAMWNKPRPESSVFDEESASGFSTMLEKQRF 369

Query: 321 YREIASAAESGWDFSTRWMRDPTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDYC 380
           +R+IA+AAESG DFSTRWMRDP + +T+ATTS++PVDLNVF+LKMELDI+ + +  GD  
Sbjct: 370 HRDIATAAESGCDFSTRWMRDPPNFTTMATTSVVPVDLNVFLLKMELDIAFMMKVSGDQN 429

Query: 381 TAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHTWDARNQNQNVYASNFIP 440
            ++ F +AS  R+K   ++FWN + GQWLDYWL + S + + TW A NQN NV+ASNF P
Sbjct: 430 GSDRFVKASKAREKAFQTVFWNEKAGQWLDYWL-SSSGEESETWKAENQNTNVFASNFAP 489

Query: 441 LWIESFYSDNKQMEKVLRSLRNSGLLCNAGIATSMINSGEQWDFPNGWAPIQHMIVEGLA 500
           +WI S  SD   ++KV+ +L+NSGL+  AGI TS+ NSG+QWD PNGWAP Q MIV GL 
Sbjct: 490 IWINSINSDENLVKKVVTALKNSGLIAPAGILTSLTNSGQQWDSPNGWAPQQEMIVTGLG 549

Query: 501 RSGLKEARALAEDIASRWLRTNYVAYKQTGYMHEKYDVQKCGDFGGGGEYVPQTGFGWSN 560
           RS +KEA+ +AEDIA RW+++NY+ YK++G +HEK  V + G++GGGGEY+PQTGFGWSN
Sbjct: 550 RSSVKEAKEMAEDIARRWIKSNYLVYKKSGTIHEKLKVTELGEYGGGGEYMPQTGFGWSN 609

Query: 561 GVVLAFLEEFGW 569
           GV+LAFLEE+GW
Sbjct: 610 GVILAFLEEYGW 618