HG10009042 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10009042
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	RING-type domain-containing protein
Location	Chr06: 1950370 .. 1952919 (-)
RNA-Seq Expression	HG10009042
Synteny	HG10009042

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTTGCCTCAAACTCCATCCCCTCCTCATCTTTACCCTCAAGCACTTCAACTTAAGCTTTATCAAGCCTTCATATTCTCCATTCCAATCCTCTTCTCCATTATTCTCTTCCTTCTTTTCTACTTGTTTTATCTCAAAAGAAGAGCTTCCTCTCTCTCTTCTTCTTCATCTTCATCTCCCCCAATTCTTCCAACTACTTCCAATACACTCACTCTCTATGTTACCACTGTAAGCAAATTATTATTCCTCTCTTTCTCAATCTTCCATTTTTCCTTTCATGTTTTTTTTTGGTGTTTCCATTGCTGTAATTTGCTTTTGAGGGGTCAGGGAAAATCTAAGCAAAAAAAGTTGAAGCTTTAATATTCATCTTTCTTTTTATTGATTTGTTTTGCCATTTTTCTTCATCTCATTGATCTTAATTGTTCAGGCTTGTCAAGTGGATTTGAAGGTAGATAATCCCATAGACAAACTTCCCAAGATCCAATTCAATGAAGAACTAAAGACTAGAGATTCTCTGTAAGTATCACCATTTCTTATTGTTAACAAAACAAAAACTCTGTTGGCTTTTTCGGGTTCTTTTGAAAGCAAAAAGGTTGTTCATGAACAGCTGTTCCTCTAAAATAAATGGGTTTGGAATACTGAATTTTTCCAAAATTTGCCAATTGATCCCTTTGCCACCCACATGCACAAATTGATATTAGATTACATGAATTCAAGAGTTTTTTTTCTAAAGGGAAGTATCTATGATATAAACTAATGCAAAATATACTTTTGGTCCCTGAAGTTTAAAGATGATGTCTATTTGATCTCTAAAGTTTAAAAACAGCATTTTTAGTCCCTAAAGTTTTAAAGATAATTCTAAATGGTCCCTAAAGTCAATTAAATGTTAACTGACTAATGGAAAGATGATGTGGTCATTTTAGAATATGATTACATAGCAAAATGAGATGAGTTCTTCTTAACCCTAAACTAATAGATGCACCAAAATATCTCTACTAGAATAACACGAAGTTAACATCGGTCCACTAAAACAGGTATTAATGTGTTACATTTTCATTGCACAAAAAATTTTCTTTTTTCCTTTATTGAGTTCATCAATTATCATAATGGAAGAACGAACTATCAATTTTTAGAATGATAAGTAAGATTTTATTCGTTGAGCTGTATCCGAATTGATACAGAAAAAGCTGGAAAGAGAGAAAAGAAAAATACAAAACAGTCAAGGGATAGGGATGGAAGATTTAGTGACTTAAATAGGGAGCACACATAACAAACAATATAAAGATAAAAGTGAAACTGATCCTTTTTCAATTTTTGTTGATTTTTTTAGATTACTTTTCTTTGGAGGATTGGAATCTCTTATAACATCCGCTCTTTAATTTCTCAAAAGTGGCTTTTAAATTCTTGGTTCTAAAAGTTACTTTTGCATTTGTTTTCTTTTCTTCTTGAAAGATGTTGTTTGTAGAACCTTAGGCCGACAAAATTGATAATGAAGTTGAAGAGCATGTTTGATTTCTATTTTTTTTTTTAATTTTTTTTTCTCTTTCTATAGTAATTATGTTGCAATCTTATTTCTAAAGTTTATTCTTTATTGTTGTATTACTTTAATAAATAGTTTGATGAAGTAATTTAAATCTCATTTTTGAGCACTACTACTAGATGGCCAAGTTTGTTATTAATTTTAAACTCTTATTTCTCTTCACACGGATCGGTCTTTTTTGTTTATGAATCCAAATTTCTCATTTCTTTGGTCTACTTTTGTACATCAATGGGATTTATGACCTAGCATAAATCTAGTAGGTAGGTATCCCAATGAGCCTAGAAAAATTTCTAATGCTACGAGAATATCACACTCTCCTTGTTCACACTATAAATTTTTCTCATAATTAACTCCACATTAATGTCAGTAGTTGCAGTCTCATTCTATTTATAAAAACTTGTTGATGTAGCATACAATAAATAAAAGAAACACGAGCCTCTCTAGCCTCTCTTCATAGTGTGTTCAACAAATGTATAGGATGAAAGATTTGAACATTTGACCTCTTGATCATAAATATATGTCTTAACTAATTGAGTTGTTTTTTTCTCTCGCCGCTTGTAATTCAATTTCTCCCATTTGTGGGAGAGAAAGATTGAGATTTCAAGTCAACCTTATATTGACCTTAGGATATGTGGGGTTAGAAATGAACATTGAAACAAAGAAAAAAGCAACAACTTTCTTATCCATTTAACAGGTGCTGCGTCTGCCTTGGAGAATTCGAGATAAAAGAGGAATTGTTGCAAGTCCCATCATGCAAACATGTGTTTCATATTGACTGTATGAACCACTGGCTCATCTCCAACTCAACTTGTCCTCTCTGTAGATGCTCAGTCATCTCAACAACCCGATGTTCTGATCTGCCCGTCATCGCACCGCCCCCGCCACCGCCACTGGCTGGCTCATCAGAGCTTCCCCTGACTTCCAACCAGACTGCAGAGATGGGATCATATTCACTCCAACATCTGCAGCTGCAACAACAGGAAGGATCAAGTCGGAGTATATGCTAA

mRNA sequence

ATGGGTTTGCCTCAAACTCCATCCCCTCCTCATCTTTACCCTCAAGCACTTCAACTTAAGCTTTATCAAGCCTTCATATTCTCCATTCCAATCCTCTTCTCCATTATTCTCTTCCTTCTTTTCTACTTGTTTTATCTCAAAAGAAGAGCTTCCTCTCTCTCTTCTTCTTCATCTTCATCTCCCCCAATTCTTCCAACTACTTCCAATACACTCACTCTCTATGTTACCACTGCTTGTCAAGTGGATTTGAAGGTAGATAATCCCATAGACAAACTTCCCAAGATCCAATTCAATGAAGAACTAAAGACTAGAGATTCTCTGTGCTGCGTCTGCCTTGGAGAATTCGAGATAAAAGAGGAATTGTTGCAAGTCCCATCATGCAAACATGTGTTTCATATTGACTGTATGAACCACTGGCTCATCTCCAACTCAACTTGTCCTCTCTGTAGATGCTCAGTCATCTCAACAACCCGATGTTCTGATCTGCCCGTCATCGCACCGCCCCCGCCACCGCCACTGGCTGGCTCATCAGAGCTTCCCCTGACTTCCAACCAGACTGCAGAGATGGGATCATATTCACTCCAACATCTGCAGCTGCAACAACAGGAAGGATCAAGTCGGAGTATATGCTAA

Coding sequence (CDS)

ATGGGTTTGCCTCAAACTCCATCCCCTCCTCATCTTTACCCTCAAGCACTTCAACTTAAGCTTTATCAAGCCTTCATATTCTCCATTCCAATCCTCTTCTCCATTATTCTCTTCCTTCTTTTCTACTTGTTTTATCTCAAAAGAAGAGCTTCCTCTCTCTCTTCTTCTTCATCTTCATCTCCCCCAATTCTTCCAACTACTTCCAATACACTCACTCTCTATGTTACCACTGCTTGTCAAGTGGATTTGAAGGTAGATAATCCCATAGACAAACTTCCCAAGATCCAATTCAATGAAGAACTAAAGACTAGAGATTCTCTGTGCTGCGTCTGCCTTGGAGAATTCGAGATAAAAGAGGAATTGTTGCAAGTCCCATCATGCAAACATGTGTTTCATATTGACTGTATGAACCACTGGCTCATCTCCAACTCAACTTGTCCTCTCTGTAGATGCTCAGTCATCTCAACAACCCGATGTTCTGATCTGCCCGTCATCGCACCGCCCCCGCCACCGCCACTGGCTGGCTCATCAGAGCTTCCCCTGACTTCCAACCAGACTGCAGAGATGGGATCATATTCACTCCAACATCTGCAGCTGCAACAACAGGAAGGATCAAGTCGGAGTATATGCTAA

Protein sequence

MGLPQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSPPILPTTSNTLTLYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEIKEELLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVISTTRCSDLPVIAPPPPPPLAGSSELPLTSNQTAEMGSYSLQHLQLQQQEGSSRSIC

Homology

[Close]

BLAST of HG10009042 vs. NCBI nr
Match: XP_038907033.1 (probable E3 ubiquitin-protein ligase RHA4A [Benincasa hispida])

HSP 1 Score: 376.7 bits (966), Expect = 1.3e-100
Identity = 198/212 (93.40%), Postives = 202/212 (95.28%), Query Frame = 0


Query: 1   MGLPQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60
           MGLPQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS
Sbjct: 1   MGLPQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60

Query: 61  PPILPTTSNTLTLYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEIKEE 120
           PPILPTTSNTLTLYVTTACQVDLK DN I+KLPKIQF+EELKTRDSLCCVCLGEFEIKE+
Sbjct: 61  PPILPTTSNTLTLYVTTACQVDLKADNLIEKLPKIQFDEELKTRDSLCCVCLGEFEIKEQ 120

Query: 121 LLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVISTTRCSDLPVIAPPPPPPLAGSSELP 180
           LLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVI TTRCSD P IA  PPPPLAGSSELP
Sbjct: 121 LLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVIPTTRCSDPPGIA--PPPPLAGSSELP 180

Query: 181 LTSNQTAEMGSYSLQHLQL--QQQEGSSRSIC 211
            TSNQTAEMGSYSLQHLQL  QQQEGSS+SIC
Sbjct: 181 PTSNQTAEMGSYSLQHLQLQQQQQEGSSQSIC 210

[Close]

BLAST of HG10009042 vs. NCBI nr
Match: XP_022928899.1 (probable E3 ubiquitin-protein ligase RHA4A [Cucurbita moschata])

HSP 1 Score: 366.7 bits (940), Expect = 1.3e-97
Identity = 192/214 (89.72%), Postives = 197/214 (92.06%), Query Frame = 0


Query: 1   MGLPQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60
           MG PQTP+PPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS
Sbjct: 1   MGQPQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60

Query: 61  ---PPILPTTSNTLTLYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEI 120
              PPILPTTSNTLTLYVTTACQVDLK DNP+DKLPKI FNEEL+TRDSLCCVCLGEFEI
Sbjct: 61  SSFPPILPTTSNTLTLYVTTACQVDLKADNPVDKLPKILFNEELRTRDSLCCVCLGEFEI 120

Query: 121 KEELLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVISTTRCSDLPVIAPPPPPPLAGSS 180
           KEELLQVPSCKHVFHIDCMNHWL SNSTCPLCRCSVI TTRC D PV+  PPPPP AGSS
Sbjct: 121 KEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRCFDPPVVVSPPPPPPAGSS 180

Query: 181 ELPLTSNQTAEMGSYSLQHLQL-QQQEGSSRSIC 211
           ELPLTSNQTAEM SYSL H QL QQQEGSS+SIC
Sbjct: 181 ELPLTSNQTAEMRSYSLLHPQLQQQQEGSSQSIC 214

[Close]

BLAST of HG10009042 vs. NCBI nr
Match: XP_022970098.1 (probable E3 ubiquitin-protein ligase RHA4A [Cucurbita maxima])

HSP 1 Score: 362.5 bits (929), Expect = 2.5e-96
Identity = 190/214 (88.79%), Postives = 196/214 (91.59%), Query Frame = 0


Query: 1   MGLPQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60
           MG PQTP+PPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS
Sbjct: 1   MGQPQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60

Query: 61  ---PPILPTTSNTLTLYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEI 120
              PPILPTTSNTLTLYVTTACQVDLK DNP+DKLPKI FNEEL+TRDSLCCVCLGEFEI
Sbjct: 61  SSFPPILPTTSNTLTLYVTTACQVDLKADNPVDKLPKILFNEELRTRDSLCCVCLGEFEI 120

Query: 121 KEELLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVISTTRCSDLPVIAPPPPPPLAGSS 180
           KEELLQVPSCKHVFHIDCMNHWL SNSTCPLCRCSVI TTRC DLPV+  PPPPP AGSS
Sbjct: 121 KEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRCFDLPVVVSPPPPPSAGSS 180

Query: 181 ELPLTSNQTAEMGSYSLQHLQL-QQQEGSSRSIC 211
           E P TS+QTAEM SYSL H QL QQQEGSS+SIC
Sbjct: 181 EPPPTSDQTAEMRSYSLLHPQLQQQQEGSSQSIC 214

[Close]

BLAST of HG10009042 vs. NCBI nr
Match: XP_023550207.1 (probable E3 ubiquitin-protein ligase RHA4A isoform X1 [Cucurbita pepo subsp. pepo] >XP_023550208.1 probable E3 ubiquitin-protein ligase RHA4A isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 360.5 bits (924), Expect = 9.6e-96
Identity = 189/218 (86.70%), Postives = 196/218 (89.91%), Query Frame = 0


Query: 1   MGLPQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60
           MG PQTP+PPHLYPQALQLKLYQAFIFSIPIL SIILFLLFYLFYLKRRASSLSSSSSSS
Sbjct: 1   MGQPQTPAPPHLYPQALQLKLYQAFIFSIPILVSIILFLLFYLFYLKRRASSLSSSSSSS 60

Query: 61  -------PPILPTTSNTLTLYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLG 120
                  PPILPTTSNTLTLY+TTACQVDLK DNP+DKLPKI FNEEL+TRDSLCCVCLG
Sbjct: 61  SSSSSSFPPILPTTSNTLTLYITTACQVDLKADNPVDKLPKILFNEELRTRDSLCCVCLG 120

Query: 121 EFEIKEELLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVISTTRCSDLPVIAPPPPPPL 180
           EFEIKEELLQVPSCKHVFHIDC+NHWL SNSTCPLCRCSVI TTRC DLPV+  PPPPP 
Sbjct: 121 EFEIKEELLQVPSCKHVFHIDCINHWLTSNSTCPLCRCSVIPTTRCFDLPVVVSPPPPPP 180

Query: 181 AGSSELPLTSNQTAEMGSYSLQHLQL-QQQEGSSRSIC 211
           AGSSELP TSNQTAEM SYSL H QL QQQEGSS+SIC
Sbjct: 181 AGSSELPPTSNQTAEMRSYSLLHPQLQQQQEGSSQSIC 218

[Close]

BLAST of HG10009042 vs. NCBI nr
Match: KAG6579565.1 (30S ribosomal protein S6 alpha, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 360.1 bits (923), Expect = 1.3e-95
Identity = 189/211 (89.57%), Postives = 194/211 (91.94%), Query Frame = 0


Query: 4   PQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS--- 63
           PQTP+PPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS   
Sbjct: 192 PQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSSSF 251

Query: 64  PPILPTTSNTLTLYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEIKEE 123
           PPILPTTSNTLTLYVTTACQVDLK DNP+DKLPKI FNEEL+TRDSLCCVCLGEFEIKEE
Sbjct: 252 PPILPTTSNTLTLYVTTACQVDLKADNPVDKLPKILFNEELRTRDSLCCVCLGEFEIKEE 311

Query: 124 LLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVISTTRCSDLPVIAPPPPPPLAGSSELP 183
           LLQVPSCKHVFHIDCMNHWL SNSTCPLCRCSVI TTRC D PV+  PPPPP AGSSELP
Sbjct: 312 LLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRCFDPPVVVSPPPPPPAGSSELP 371

Query: 184 LTSNQTAEMGSYSLQHLQL-QQQEGSSRSIC 211
            TSNQTAEM SYSL H QL QQQEGSS+SIC
Sbjct: 372 PTSNQTAEMRSYSLLHPQLQQQQEGSSQSIC 402

[Close]

BLAST of HG10009042 vs. ExPASy Swiss-Prot
Match: Q84TF5 (Probable E3 ubiquitin-protein ligase RHA4A OS=Arabidopsis thaliana OX=3702 GN=RHA4A PE=2 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 1.4e-49
Identity = 108/159 (67.92%), Postives = 131/159 (82.39%), Query Frame = 0


Query: 4   PQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSPPI 63
           PQ+P  PHLYPQA+QLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSL   SS SP I
Sbjct: 4   PQSPITPHLYPQAIQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSL---SSPSPMI 63

Query: 64  LPTTSNTLT-LYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEIKEELL 123
           LP +S+  T  ++ + C +D+KV+   DKL  + FNEEL TRDSLCCVCLGEFE+KEEL+
Sbjct: 64  LPVSSSHQTSSHLPSVCLLDVKVELK-DKLHVVLFNEELGTRDSLCCVCLGEFELKEELV 123

Query: 124 QVPSCKHVFHIDCMNHWLISNSTCPLCRCSV-ISTTRCS 161
           ++P CKH+FH+DC++ WL S++TCPLCR SV IS+T+ S
Sbjct: 124 EMPLCKHIFHLDCIHLWLYSHNTCPLCRSSVSISSTKTS 158

[Close]

BLAST of HG10009042 vs. ExPASy Swiss-Prot
Match: Q570X5 (RING-H2 finger protein ATL58 OS=Arabidopsis thaliana OX=3702 GN=ATL58 PE=2 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.3e-23
Identity = 59/137 (43.07%), Postives = 87/137 (63.50%), Query Frame = 0


Query: 18  QLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSPPILPTTSNTLTLYVTT 77
           +LKLYQAFIFS+PI F+ I+  LFYL YL+R +S LSS    +  I   + +T+ L ++ 
Sbjct: 19  ELKLYQAFIFSVPICFTFIILFLFYLIYLRRSSSDLSSLGMRTTFIPGNSLSTIELGLSK 78

Query: 78  ACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEIKEELLQVPSCKHVFHIDCMN 137
             +         + LP + F E     DS C VCLG+++  ++L Q+P CKH FH+DC++
Sbjct: 79  ELR---------EMLPIVVFKESFTVMDSQCSVCLGDYQPNDKLQQIPVCKHTFHMDCID 138

Query: 138 HWLISNSTCPLCRCSVI 155
            WL S++TCPLCR ++I
Sbjct: 139 LWLTSHTTCPLCRLALI 146

[Close]

BLAST of HG10009042 vs. ExPASy Swiss-Prot
Match: Q9SN28 (RING-H2 finger protein ATL7 OS=Arabidopsis thaliana OX=3702 GN=ATL7 PE=2 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 2.1e-21
Identity = 60/156 (38.46%), Postives = 97/156 (62.18%), Query Frame = 0


Query: 3   LPQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASS---LSSSSSS 62
           +P+T +P +   ++ +LKLYQAFIFS+PI F+ I+  + Y+ YL+R +++    SS    
Sbjct: 11  IPETYAPSN-STESEKLKLYQAFIFSVPICFTFIVLFVLYVIYLRRNSTTNVDWSSLGMR 70

Query: 63  SPPILPTTSNTLTLYVTTACQVDLKVDNPI-DKLPKIQFNEELKTRDSLCCVCLGEFEIK 122
               +PT +N  T         +L +   I + LP + + E    +DS C VCLG+++ +
Sbjct: 71  GGTFVPTNNNLST--------AELGLSKDIREMLPVVIYKESFIVKDSQCSVCLGDYQAE 130

Query: 123 EELLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVI 155
           E+L Q+PSC H FH++C++ WL S++TCPLCR S+I
Sbjct: 131 EKLQQMPSCGHTFHMECIDLWLTSHTTCPLCRLSLI 157

[Close]

BLAST of HG10009042 vs. ExPASy Swiss-Prot
Match: Q9SN27 (E3 ubiquitin-protein ligase ATL59 OS=Arabidopsis thaliana OX=3702 GN=ATL59 PE=1 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 1.9e-14
Identity = 48/131 (36.64%), Postives = 76/131 (58.02%), Query Frame = 0


Query: 25  FIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSPPILPTTSNTLTLYVTTACQVDLK 84
           FI  +PI   +I+ L+ Y+    RR S+ +   SS    +PT +N  T         +L 
Sbjct: 24  FIVCVPICVILIVLLVLYIM---RRNSNTNVDWSSLGGFVPTNNNLST--------AELG 83

Query: 85  VDNPI-DKLPKIQFNEELKTRDSLCCVCLGEFEIKEELLQVPSCKHVFHIDCMNHWLISN 144
           +   I + LP + + E     D+ C VCLG+++ +E+L Q+PSC H FH++C++ WL S+
Sbjct: 84  LSKDIREMLPIVIYKESFTVNDTQCSVCLGDYQAEEKLQQMPSCGHTFHMECIDLWLTSH 143

Query: 145 STCPLCRCSVI 155
           +TCPLCR S+I
Sbjct: 144 TTCPLCRLSLI 143

[Close]

BLAST of HG10009042 vs. ExPASy Swiss-Prot
Match: Q8GW38 (RING-H2 finger protein ATL47 OS=Arabidopsis thaliana OX=3702 GN=ATL47 PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 3.1e-12
Identity = 54/141 (38.30%), Postives = 80/141 (56.74%), Query Frame = 0


Query: 26  IFSIPILFSIILFL-----LFYLFYLKRRASSLSSS---SSSSPPILPTTSNTLTLYVTT 85
           I  I +L S+I F+     L   +YLK++ S+LSSS   S+ +P    + S+T    +  
Sbjct: 53  ILFIIVLLSVIFFICSILHLLVRYYLKKKRSNLSSSPNESNQNPEF--SDSDTYQRQLQQ 112

Query: 86  ACQV-DLKVDNP-IDKLPKIQFNEELKTRDSL-CCVCLGEFEIKEELLQVPSCKHVFHID 145
              + D  +D   ID LP   + E   T++   C VCL EF   ++L  +P+C H FHID
Sbjct: 113 LFHLHDSGLDQALIDALPVFLYKEIKGTKEPFDCAVCLCEFSEDDKLRLLPNCSHAFHID 172

Query: 146 CMNHWLISNSTCPLCRCSVIS 156
           C++ WL+SNSTCPLCR ++ S
Sbjct: 173 CIDTWLLSNSTCPLCRGTLFS 191

[Close]

BLAST of HG10009042 vs. ExPASy TrEMBL
Match: A0A6J1EM64 (probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita moschata OX=3662 GN=LOC111435669 PE=4 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 6.5e-98
Identity = 192/214 (89.72%), Postives = 197/214 (92.06%), Query Frame = 0


Query: 1   MGLPQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60
           MG PQTP+PPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS
Sbjct: 1   MGQPQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60

Query: 61  ---PPILPTTSNTLTLYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEI 120
              PPILPTTSNTLTLYVTTACQVDLK DNP+DKLPKI FNEEL+TRDSLCCVCLGEFEI
Sbjct: 61  SSFPPILPTTSNTLTLYVTTACQVDLKADNPVDKLPKILFNEELRTRDSLCCVCLGEFEI 120

Query: 121 KEELLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVISTTRCSDLPVIAPPPPPPLAGSS 180
           KEELLQVPSCKHVFHIDCMNHWL SNSTCPLCRCSVI TTRC D PV+  PPPPP AGSS
Sbjct: 121 KEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRCFDPPVVVSPPPPPPAGSS 180

Query: 181 ELPLTSNQTAEMGSYSLQHLQL-QQQEGSSRSIC 211
           ELPLTSNQTAEM SYSL H QL QQQEGSS+SIC
Sbjct: 181 ELPLTSNQTAEMRSYSLLHPQLQQQQEGSSQSIC 214

[Close]

BLAST of HG10009042 vs. ExPASy TrEMBL
Match: A0A6J1I2V6 (probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita maxima OX=3661 GN=LOC111469090 PE=4 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 1.2e-96
Identity = 190/214 (88.79%), Postives = 196/214 (91.59%), Query Frame = 0


Query: 1   MGLPQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60
           MG PQTP+PPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS
Sbjct: 1   MGQPQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60

Query: 61  ---PPILPTTSNTLTLYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEI 120
              PPILPTTSNTLTLYVTTACQVDLK DNP+DKLPKI FNEEL+TRDSLCCVCLGEFEI
Sbjct: 61  SSFPPILPTTSNTLTLYVTTACQVDLKADNPVDKLPKILFNEELRTRDSLCCVCLGEFEI 120

Query: 121 KEELLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVISTTRCSDLPVIAPPPPPPLAGSS 180
           KEELLQVPSCKHVFHIDCMNHWL SNSTCPLCRCSVI TTRC DLPV+  PPPPP AGSS
Sbjct: 121 KEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRCFDLPVVVSPPPPPSAGSS 180

Query: 181 ELPLTSNQTAEMGSYSLQHLQL-QQQEGSSRSIC 211
           E P TS+QTAEM SYSL H QL QQQEGSS+SIC
Sbjct: 181 EPPPTSDQTAEMRSYSLLHPQLQQQQEGSSQSIC 214

[Close]

BLAST of HG10009042 vs. ExPASy TrEMBL
Match: A0A6J1H2A4 (probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita moschata OX=3662 GN=LOC111459379 PE=4 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 2.8e-93
Identity = 188/219 (85.84%), Postives = 196/219 (89.50%), Query Frame = 0


Query: 1   MGLPQTP-SPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSS 60
           MG+PQTP +PPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSS
Sbjct: 1   MGVPQTPAAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSS 60

Query: 61  S-PPILPTTSNTLTLYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEIK 120
           S PPILPTTSN LTLYVTTAC+VDLK DNP+DKLPKI FNEEL++RDSLCCVCLGEFEIK
Sbjct: 61  SPPPILPTTSNRLTLYVTTACRVDLKADNPMDKLPKIFFNEELRSRDSLCCVCLGEFEIK 120

Query: 121 EELLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVISTTRCSDLPVIA-------PPPPP 180
           EELLQVPSCKHVFHIDCMN WL SNSTCPLCRCSVI TTRCSD PV+        PPPPP
Sbjct: 121 EELLQVPSCKHVFHIDCMNLWLASNSTCPLCRCSVIPTTRCSDPPVLVPPQLTPPPPPPP 180

Query: 181 PLAGSSELPLTSNQTAEMGSYSLQHLQLQQQEGSSRSIC 211
           PLA S ELPL SNQTAEM SYSLQH QLQQQ GSS+SIC
Sbjct: 181 PLAASLELPLASNQTAEMVSYSLQHPQLQQQIGSSQSIC 219

[Close]

BLAST of HG10009042 vs. ExPASy TrEMBL
Match: A0A6J1DWP0 (probable E3 ubiquitin-protein ligase RHA4A OS=Momordica charantia OX=3673 GN=LOC111023829 PE=4 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 2.4e-92
Identity = 182/210 (86.67%), Postives = 189/210 (90.00%), Query Frame = 0


Query: 1   MGLPQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60
           MGLPQTP+PPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLS SSSSS
Sbjct: 1   MGLPQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSFSSSSS 60

Query: 61  PPILPTTSNTLTLYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEIKEE 120
           PPILPT+SN LTLYVTTACQVD K DNPI+KLPKI FNEEL+ RDSLCCVCLGEFEIKEE
Sbjct: 61  PPILPTSSNALTLYVTTACQVDSKADNPIEKLPKILFNEELRARDSLCCVCLGEFEIKEE 120

Query: 121 LLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVISTTRCSDLPVIAPPPPPPLAGSSELP 180
           LLQVPSCKHVFHIDCMNHWL +NSTCPLCRCSVI   RC D PV AP P  PL   SELP
Sbjct: 121 LLQVPSCKHVFHIDCMNHWLTTNSTCPLCRCSVIPPIRCPDPPVAAPSPTLPL--PSELP 180

Query: 181 LTSNQTAEMGSYSLQHLQLQQQEGSSRSIC 211
           LTSNQTAEM SYSL+H QLQQQEGSS+SIC
Sbjct: 181 LTSNQTAEMVSYSLRHPQLQQQEGSSQSIC 208

[Close]

BLAST of HG10009042 vs. ExPASy TrEMBL
Match: A0A6J1K5R3 (probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita maxima OX=3661 GN=LOC111490976 PE=4 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 3.1e-92
Identity = 187/218 (85.78%), Postives = 195/218 (89.45%), Query Frame = 0


Query: 1   MGLPQT-PSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSS 60
           MG+PQT  +PPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSS
Sbjct: 1   MGVPQTSAAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSS 60

Query: 61  S--PPILPTTSNTLTLYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEI 120
           S  PPILPTTSN LTLYVTTAC+VDLK +NPIDKLPKI FNEEL++RDSLCCVCLGEFEI
Sbjct: 61  SPPPPILPTTSNRLTLYVTTACRVDLKANNPIDKLPKIFFNEELRSRDSLCCVCLGEFEI 120

Query: 121 KEELLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVISTTRCSDLPV-----IAPPPPPP 180
           KEELLQVPSCKHVFHIDCMN WL SNSTCPLCRCSVI TTRCSD PV     + PPPPPP
Sbjct: 121 KEELLQVPSCKHVFHIDCMNLWLASNSTCPLCRCSVIPTTRCSDPPVLVPPQLTPPPPPP 180

Query: 181 LAGSSELPLTSNQTAEMGSYSLQHLQLQQQEGSSRSIC 211
           LA S ELPL SNQTAEM SYSLQH QLQQQ GSS+SIC
Sbjct: 181 LAASLELPLASNQTAEMVSYSLQHPQLQQQIGSSQSIC 218

[Close]

BLAST of HG10009042 vs. TAIR 10
Match: AT4G24015.1 (RING/U-box superfamily protein )

HSP 1 Score: 197.6 bits (501), Expect = 1.0e-50
Identity = 108/159 (67.92%), Postives = 131/159 (82.39%), Query Frame = 0


Query: 4   PQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSPPI 63
           PQ+P  PHLYPQA+QLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSL   SS SP I
Sbjct: 4   PQSPITPHLYPQAIQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSL---SSPSPMI 63

Query: 64  LPTTSNTLT-LYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEIKEELL 123
           LP +S+  T  ++ + C +D+KV+   DKL  + FNEEL TRDSLCCVCLGEFE+KEEL+
Sbjct: 64  LPVSSSHQTSSHLPSVCLLDVKVELK-DKLHVVLFNEELGTRDSLCCVCLGEFELKEELV 123

Query: 124 QVPSCKHVFHIDCMNHWLISNSTCPLCRCSV-ISTTRCS 161
           ++P CKH+FH+DC++ WL S++TCPLCR SV IS+T+ S
Sbjct: 124 EMPLCKHIFHLDCIHLWLYSHNTCPLCRSSVSISSTKTS 158

[Close]

BLAST of HG10009042 vs. TAIR 10
Match: AT1G33480.1 (RING/U-box superfamily protein )

HSP 1 Score: 111.3 bits (277), Expect = 9.5e-25
Identity = 59/137 (43.07%), Postives = 87/137 (63.50%), Query Frame = 0


Query: 18  QLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSPPILPTTSNTLTLYVTT 77
           +LKLYQAFIFS+PI F+ I+  LFYL YL+R +S LSS    +  I   + +T+ L ++ 
Sbjct: 19  ELKLYQAFIFSVPICFTFIILFLFYLIYLRRSSSDLSSLGMRTTFIPGNSLSTIELGLSK 78

Query: 78  ACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEIKEELLQVPSCKHVFHIDCMN 137
             +         + LP + F E     DS C VCLG+++  ++L Q+P CKH FH+DC++
Sbjct: 79  ELR---------EMLPIVVFKESFTVMDSQCSVCLGDYQPNDKLQQIPVCKHTFHMDCID 138

Query: 138 HWLISNSTCPLCRCSVI 155
            WL S++TCPLCR ++I
Sbjct: 139 LWLTSHTTCPLCRLALI 146

[Close]

BLAST of HG10009042 vs. TAIR 10
Match: AT4G10150.1 (RING/U-box superfamily protein )

HSP 1 Score: 104.0 bits (258), Expect = 1.5e-22
Identity = 60/156 (38.46%), Postives = 97/156 (62.18%), Query Frame = 0


Query: 3   LPQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASS---LSSSSSS 62
           +P+T +P +   ++ +LKLYQAFIFS+PI F+ I+  + Y+ YL+R +++    SS    
Sbjct: 11  IPETYAPSN-STESEKLKLYQAFIFSVPICFTFIVLFVLYVIYLRRNSTTNVDWSSLGMR 70

Query: 63  SPPILPTTSNTLTLYVTTACQVDLKVDNPI-DKLPKIQFNEELKTRDSLCCVCLGEFEIK 122
               +PT +N  T         +L +   I + LP + + E    +DS C VCLG+++ +
Sbjct: 71  GGTFVPTNNNLST--------AELGLSKDIREMLPVVIYKESFIVKDSQCSVCLGDYQAE 130

Query: 123 EELLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVI 155
           E+L Q+PSC H FH++C++ WL S++TCPLCR S+I
Sbjct: 131 EKLQQMPSCGHTFHMECIDLWLTSHTTCPLCRLSLI 157

[Close]

BLAST of HG10009042 vs. TAIR 10
Match: AT4G10160.1 (RING/U-box superfamily protein )

HSP 1 Score: 80.9 bits (198), Expect = 1.4e-15
Identity = 48/131 (36.64%), Postives = 76/131 (58.02%), Query Frame = 0


Query: 25  FIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSPPILPTTSNTLTLYVTTACQVDLK 84
           FI  +PI   +I+ L+ Y+    RR S+ +   SS    +PT +N  T         +L 
Sbjct: 24  FIVCVPICVILIVLLVLYIM---RRNSNTNVDWSSLGGFVPTNNNLST--------AELG 83

Query: 85  VDNPI-DKLPKIQFNEELKTRDSLCCVCLGEFEIKEELLQVPSCKHVFHIDCMNHWLISN 144
           +   I + LP + + E     D+ C VCLG+++ +E+L Q+PSC H FH++C++ WL S+
Sbjct: 84  LSKDIREMLPIVIYKESFTVNDTQCSVCLGDYQAEEKLQQMPSCGHTFHMECIDLWLTSH 143

Query: 145 STCPLCRCSVI 155
           +TCPLCR S+I
Sbjct: 144 TTCPLCRLSLI 143

[Close]

BLAST of HG10009042 vs. TAIR 10
Match: AT1G23980.1 (RING/U-box superfamily protein )

HSP 1 Score: 73.6 bits (179), Expect = 2.2e-13
Identity = 54/141 (38.30%), Postives = 80/141 (56.74%), Query Frame = 0


Query: 26  IFSIPILFSIILFL-----LFYLFYLKRRASSLSSS---SSSSPPILPTTSNTLTLYVTT 85
           I  I +L S+I F+     L   +YLK++ S+LSSS   S+ +P    + S+T    +  
Sbjct: 53  ILFIIVLLSVIFFICSILHLLVRYYLKKKRSNLSSSPNESNQNPEF--SDSDTYQRQLQQ 112

Query: 86  ACQV-DLKVDNP-IDKLPKIQFNEELKTRDSL-CCVCLGEFEIKEELLQVPSCKHVFHID 145
              + D  +D   ID LP   + E   T++   C VCL EF   ++L  +P+C H FHID
Sbjct: 113 LFHLHDSGLDQALIDALPVFLYKEIKGTKEPFDCAVCLCEFSEDDKLRLLPNCSHAFHID 172

Query: 146 CMNHWLISNSTCPLCRCSVIS 156
           C++ WL+SNSTCPLCR ++ S
Sbjct: 173 CIDTWLLSNSTCPLCRGTLFS 191

The following BLAST results are available for this feature:

BLAST of HG10009042 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038907033.1	1.3e-100	93.40	probable E3 ubiquitin-protein ligase RHA4A [Benincasa hispida]	[more]
XP_022928899.1	1.3e-97	89.72	probable E3 ubiquitin-protein ligase RHA4A [Cucurbita moschata]	[more]
XP_022970098.1	2.5e-96	88.79	probable E3 ubiquitin-protein ligase RHA4A [Cucurbita maxima]	[more]
XP_023550207.1	9.6e-96	86.70	probable E3 ubiquitin-protein ligase RHA4A isoform X1 [Cucurbita pepo subsp. pep...	[more]
KAG6579565.1	1.3e-95	89.57	30S ribosomal protein S6 alpha, chloroplastic, partial [Cucurbita argyrosperma s...	[more]

BLAST of HG10009042 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84TF5	1.4e-49	67.92	Probable E3 ubiquitin-protein ligase RHA4A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q570X5	1.3e-23	43.07	RING-H2 finger protein ATL58 OS=Arabidopsis thaliana OX=3702 GN=ATL58 PE=2 SV=1	[more]
Q9SN28	2.1e-21	38.46	RING-H2 finger protein ATL7 OS=Arabidopsis thaliana OX=3702 GN=ATL7 PE=2 SV=1	[more]
Q9SN27	1.9e-14	36.64	E3 ubiquitin-protein ligase ATL59 OS=Arabidopsis thaliana OX=3702 GN=ATL59 PE=1 ...	[more]
Q8GW38	3.1e-12	38.30	RING-H2 finger protein ATL47 OS=Arabidopsis thaliana OX=3702 GN=ATL47 PE=2 SV=1	[more]

BLAST of HG10009042 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EM64	6.5e-98	89.72	probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1I2V6	1.2e-96	88.79	probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1H2A4	2.8e-93	85.84	probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1DWP0	2.4e-92	86.67	probable E3 ubiquitin-protein ligase RHA4A OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A6J1K5R3	3.1e-92	85.78	probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]

BLAST of HG10009042 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G24015.1	1.0e-50	67.92	RING/U-box superfamily protein	[more]
AT1G33480.1	9.5e-25	43.07	RING/U-box superfamily protein	[more]
AT4G10150.1	1.5e-22	38.46	RING/U-box superfamily protein	[more]
AT4G10160.1	1.4e-15	36.64	RING/U-box superfamily protein	[more]
AT1G23980.1	2.2e-13	38.30	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 108..149 e-value: 0.002 score: 27.4
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 108..150 e-value: 1.8E-11 score: 44.2
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 108..150 score: 11.766381
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 76..155 e-value: 9.9E-17 score: 62.4
None	No IPR available	PANTHER	PTHR22765	RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING	coord: 4..164
None	No IPR available	PANTHER	PTHR22765:SF187	E3 UBIQUITIN-PROTEIN LIGASE RHA4A-RELATED	coord: 4..164
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 88..157

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10009042.1	HG10009042.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0061630	ubiquitin protein ligase activity