HG10009012 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10009012
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	methionine gamma-lyase-like
Location	Chr06: 1688816 .. 1689149 (-)
RNA-Seq Expression	HG10009012
Synteny	HG10009012

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGACTGAAGAGAGAGCCAATAGGCTCATGAATCTCCTTCAAAACTATACACATTTCGGTCTCATGGCTGTTAGCTTGGGCTTCTTCGAGACTCTCATGTCATGCTCAGGTAGGGTTAAACCATTCCTAGTGAAGCTAATAGGTTATGATAAATTTAACTATTTATTCTTTTTTTTTTACAGGTAGTAGCACTAGTAGCGAGTTGAACTGCGAGGAGAAGGAATTGGCTGGCATTTCTCCCGGATTGATTAGGATGTCAGTTGGATATGTTGGAACCTTAGAGCAGAAATGGAGTCAATTGGAGAAGGCGCTGTTGAAGATGAAGATTTGA

mRNA sequence

ATGGGGACTGAAGAGAGAGCCAATAGGCTCATGAATCTCCTTCAAAACTATACACATTTCGGTCTCATGGCTGTTAGCTTGGGCTTCTTCGAGACTCTCATGTCATGCTCAGGTAGTAGCACTAGTAGCGAGTTGAACTGCGAGGAGAAGGAATTGGCTGGCATTTCTCCCGGATTGATTAGGATGTCAGTTGGATATGTTGGAACCTTAGAGCAGAAATGGAGTCAATTGGAGAAGGCGCTGTTGAAGATGAAGATTTGA

Coding sequence (CDS)

ATGGGGACTGAAGAGAGAGCCAATAGGCTCATGAATCTCCTTCAAAACTATACACATTTCGGTCTCATGGCTGTTAGCTTGGGCTTCTTCGAGACTCTCATGTCATGCTCAGGTAGTAGCACTAGTAGCGAGTTGAACTGCGAGGAGAAGGAATTGGCTGGCATTTCTCCCGGATTGATTAGGATGTCAGTTGGATATGTTGGAACCTTAGAGCAGAAATGGAGTCAATTGGAGAAGGCGCTGTTGAAGATGAAGATTTGA

Protein sequence

MGTEERANRLMNLLQNYTHFGLMAVSLGFFETLMSCSGSSTSSELNCEEKELAGISPGLIRMSVGYVGTLEQKWSQLEKALLKMKI

Homology

[Close]

BLAST of HG10009012 vs. NCBI nr
Match: XP_038875936.1 (methionine gamma-lyase-like [Benincasa hispida])

HSP 1 Score: 163.7 bits (413), Expect = 7.1e-37
Identity = 83/86 (96.51%), Postives = 85/86 (98.84%), Query Frame = 0


Query: 1   MGTEERANRLMNLLQNYTHFGLMAVSLGFFETLMSCSGSSTSSELNCEEKELAGISPGLI 60
           MGTEERANRLMNLLQN+THFGLMAVSLGFFETLMSCSGSSTSSELN +EKELAGISPGLI
Sbjct: 358 MGTEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNSKEKELAGISPGLI 417

Query: 61  RMSVGYVGTLEQKWSQLEKALLKMKI 87
           RMSVGYVGTLEQKWSQLEKALLKMKI
Sbjct: 418 RMSVGYVGTLEQKWSQLEKALLKMKI 443

[Close]

BLAST of HG10009012 vs. NCBI nr
Match: XP_004143943.3 (methionine gamma-lyase [Cucumis sativus] >KGN49981.1 hypothetical protein Csa_000014 [Cucumis sativus])

HSP 1 Score: 163.3 bits (412), Expect = 9.2e-37
Identity = 83/86 (96.51%), Postives = 85/86 (98.84%), Query Frame = 0


Query: 1   MGTEERANRLMNLLQNYTHFGLMAVSLGFFETLMSCSGSSTSSELNCEEKELAGISPGLI 60
           MGTEERANRLMNLLQN+THFGLMAVSLGFFETLMSCSGSSTSSELN EEKELAGISPGLI
Sbjct: 357 MGTEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLI 416

Query: 61  RMSVGYVGTLEQKWSQLEKALLKMKI 87
           RMSVGYVGTLEQKW+QLEKALLKMKI
Sbjct: 417 RMSVGYVGTLEQKWNQLEKALLKMKI 442

[Close]

BLAST of HG10009012 vs. NCBI nr
Match: XP_008437446.1 (PREDICTED: methionine gamma-lyase-like [Cucumis melo] >KAA0042648.1 methionine gamma-lyase-like [Cucumis melo var. makuwa] >TYK06049.1 methionine gamma-lyase-like [Cucumis melo var. makuwa])

HSP 1 Score: 162.9 bits (411), Expect = 1.2e-36
Identity = 83/85 (97.65%), Postives = 84/85 (98.82%), Query Frame = 0


Query: 1   MGTEERANRLMNLLQNYTHFGLMAVSLGFFETLMSCSGSSTSSELNCEEKELAGISPGLI 60
           MGTEERANRLMNLLQN+THFGLMAVSLGFFETLMSCSGSSTSSELN EEKELAGISPGLI
Sbjct: 357 MGTEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLI 416

Query: 61  RMSVGYVGTLEQKWSQLEKALLKMK 86
           RMSVGYVGTLEQKWSQLEKALLKMK
Sbjct: 417 RMSVGYVGTLEQKWSQLEKALLKMK 441

[Close]

BLAST of HG10009012 vs. NCBI nr
Match: KAG7017043.1 (Methionine gamma-lyase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 160.2 bits (404), Expect = 7.8e-36
Identity = 80/86 (93.02%), Postives = 84/86 (97.67%), Query Frame = 0


Query: 1   MGTEERANRLMNLLQNYTHFGLMAVSLGFFETLMSCSGSSTSSELNCEEKELAGISPGLI 60
           MGTEERANRLMNLLQN+THFGL+AVSLG+FETLMSCSGSSTSSE+N EEKELAGISPGLI
Sbjct: 351 MGTEERANRLMNLLQNHTHFGLLAVSLGYFETLMSCSGSSTSSEMNVEEKELAGISPGLI 410

Query: 61  RMSVGYVGTLEQKWSQLEKALLKMKI 87
           RMSVGYVGTLEQKWSQLEKALLKM I
Sbjct: 411 RMSVGYVGTLEQKWSQLEKALLKMNI 436

[Close]

BLAST of HG10009012 vs. NCBI nr
Match: KAG6579584.1 (Methionine gamma-lyase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 160.2 bits (404), Expect = 7.8e-36
Identity = 80/86 (93.02%), Postives = 84/86 (97.67%), Query Frame = 0


Query: 1   MGTEERANRLMNLLQNYTHFGLMAVSLGFFETLMSCSGSSTSSELNCEEKELAGISPGLI 60
           MGTEERANRLMNLLQN+THFGL+AVSLG+FETLMSCSGSSTSSE+N EEKELAGISPGLI
Sbjct: 351 MGTEERANRLMNLLQNHTHFGLLAVSLGYFETLMSCSGSSTSSEMNVEEKELAGISPGLI 410

Query: 61  RMSVGYVGTLEQKWSQLEKALLKMKI 87
           RMSVGYVGTLEQKWSQLEKALLKM I
Sbjct: 411 RMSVGYVGTLEQKWSQLEKALLKMNI 436

[Close]

BLAST of HG10009012 vs. ExPASy Swiss-Prot
Match: Q9SGU9 (Methionine gamma-lyase OS=Arabidopsis thaliana OX=3702 GN=MGL PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 1.9e-29
Identity = 64/84 (76.19%), Postives = 73/84 (86.90%), Query Frame = 0


Query: 1   MGTEERANRLMNLLQNYTHFGLMAVSLGFFETLMSCSGSSTSSELNCEEKELAGISPGLI 60
           M TEE+AN+LM  LQN T FG MAVSLG++ETLMSCSGSSTSSEL+  +KE AGISPGL+
Sbjct: 358 METEEKANKLMAYLQNATQFGFMAVSLGYYETLMSCSGSSTSSELDPSQKEAAGISPGLV 417

Query: 61  RMSVGYVGTLEQKWSQLEKALLKM 85
           RMSVGYVGTLEQKW+Q EKA L+M
Sbjct: 418 RMSVGYVGTLEQKWTQFEKAFLRM 441

[Close]

BLAST of HG10009012 vs. ExPASy Swiss-Prot
Match: Q8RDT4 (L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / DSM 15643 / BCRC 10681 / CIP 101130 / JCM 8532 / KCTC 2640 / LMG 13131 / VPI 4355) OX=190304 GN=FN1419 PE=1 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 6.3e-04
Identity = 28/62 (45.16%), Postives = 40/62 (64.52%), Query Frame = 0


Query: 23  MAVSLGFFETLMSCSGSSTSSELNCEEKELAGISPGLIRMSVGYVGTLEQKWSQLEKALL 82
           +AVSLG  ETL+    S T S    EE+E+AGI+ GL+R+SVG +  +E   + LE+ L 
Sbjct: 335 LAVSLGDTETLIQHPASMTHSPYTKEEREVAGITDGLVRLSVG-LENVEDIIADLEQGLE 394

Query: 83  KM 85
           K+
Sbjct: 395 KI 395

[Close]

BLAST of HG10009012 vs. ExPASy TrEMBL
Match: A0A0A0KQL7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G148520 PE=3 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 4.5e-37
Identity = 83/86 (96.51%), Postives = 85/86 (98.84%), Query Frame = 0


Query: 1   MGTEERANRLMNLLQNYTHFGLMAVSLGFFETLMSCSGSSTSSELNCEEKELAGISPGLI 60
           MGTEERANRLMNLLQN+THFGLMAVSLGFFETLMSCSGSSTSSELN EEKELAGISPGLI
Sbjct: 357 MGTEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLI 416

Query: 61  RMSVGYVGTLEQKWSQLEKALLKMKI 87
           RMSVGYVGTLEQKW+QLEKALLKMKI
Sbjct: 417 RMSVGYVGTLEQKWNQLEKALLKMKI 442

[Close]

BLAST of HG10009012 vs. ExPASy TrEMBL
Match: A0A5A7TIP4 (Methionine gamma-lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001500 PE=3 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 5.8e-37
Identity = 83/85 (97.65%), Postives = 84/85 (98.82%), Query Frame = 0


Query: 1   MGTEERANRLMNLLQNYTHFGLMAVSLGFFETLMSCSGSSTSSELNCEEKELAGISPGLI 60
           MGTEERANRLMNLLQN+THFGLMAVSLGFFETLMSCSGSSTSSELN EEKELAGISPGLI
Sbjct: 357 MGTEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLI 416

Query: 61  RMSVGYVGTLEQKWSQLEKALLKMK 86
           RMSVGYVGTLEQKWSQLEKALLKMK
Sbjct: 417 RMSVGYVGTLEQKWSQLEKALLKMK 441

[Close]

BLAST of HG10009012 vs. ExPASy TrEMBL
Match: A0A1S3AU15 (methionine gamma-lyase-like OS=Cucumis melo OX=3656 GN=LOC103482860 PE=3 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 5.8e-37
Identity = 83/85 (97.65%), Postives = 84/85 (98.82%), Query Frame = 0


Query: 1   MGTEERANRLMNLLQNYTHFGLMAVSLGFFETLMSCSGSSTSSELNCEEKELAGISPGLI 60
           MGTEERANRLMNLLQN+THFGLMAVSLGFFETLMSCSGSSTSSELN EEKELAGISPGLI
Sbjct: 357 MGTEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLI 416

Query: 61  RMSVGYVGTLEQKWSQLEKALLKMK 86
           RMSVGYVGTLEQKWSQLEKALLKMK
Sbjct: 417 RMSVGYVGTLEQKWSQLEKALLKMK 441

[Close]

BLAST of HG10009012 vs. ExPASy TrEMBL
Match: A0A6J1I2P5 (methionine gamma-lyase-like OS=Cucurbita maxima OX=3661 GN=LOC111469051 PE=3 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 3.5e-34
Identity = 76/86 (88.37%), Postives = 82/86 (95.35%), Query Frame = 0


Query: 1   MGTEERANRLMNLLQNYTHFGLMAVSLGFFETLMSCSGSSTSSELNCEEKELAGISPGLI 60
           MGTEERANRLMNLLQN+THFG +AVSLG+FETL+SCSGSSTSSE+N EEKELAGISPGLI
Sbjct: 346 MGTEERANRLMNLLQNHTHFGFLAVSLGYFETLISCSGSSTSSEMNVEEKELAGISPGLI 405

Query: 61  RMSVGYVGTLEQKWSQLEKALLKMKI 87
           RMSV YVGTLEQKWSQLEKALLK+ I
Sbjct: 406 RMSVAYVGTLEQKWSQLEKALLKLNI 431

[Close]

BLAST of HG10009012 vs. ExPASy TrEMBL
Match: A0A6J1E0P6 (methionine gamma-lyase OS=Momordica charantia OX=3673 GN=LOC111026086 PE=3 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 3.5e-34
Identity = 77/86 (89.53%), Postives = 82/86 (95.35%), Query Frame = 0


Query: 1   MGTEERANRLMNLLQNYTHFGLMAVSLGFFETLMSCSGSSTSSELNCEEKELAGISPGLI 60
           M TEERANRLMNLLQN+T FGLMAVSLGFFETLMSCSGSSTSSELN EEKELAGISPGL+
Sbjct: 358 METEERANRLMNLLQNHTKFGLMAVSLGFFETLMSCSGSSTSSELNAEEKELAGISPGLV 417

Query: 61  RMSVGYVGTLEQKWSQLEKALLKMKI 87
           R+SVGYVGTLEQKWSQLE+ LLKMK+
Sbjct: 418 RISVGYVGTLEQKWSQLERGLLKMKL 443

[Close]

BLAST of HG10009012 vs. TAIR 10
Match: AT1G64660.1 (methionine gamma-lyase )

HSP 1 Score: 129.4 bits (324), Expect = 1.4e-30
Identity = 64/84 (76.19%), Postives = 73/84 (86.90%), Query Frame = 0


Query: 1   MGTEERANRLMNLLQNYTHFGLMAVSLGFFETLMSCSGSSTSSELNCEEKELAGISPGLI 60
           M TEE+AN+LM  LQN T FG MAVSLG++ETLMSCSGSSTSSEL+  +KE AGISPGL+
Sbjct: 358 METEEKANKLMAYLQNATQFGFMAVSLGYYETLMSCSGSSTSSELDPSQKEAAGISPGLV 417

Query: 61  RMSVGYVGTLEQKWSQLEKALLKM 85
           RMSVGYVGTLEQKW+Q EKA L+M
Sbjct: 418 RMSVGYVGTLEQKWTQFEKAFLRM 441

The following BLAST results are available for this feature:

BLAST of HG10009012 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038875936.1	7.1e-37	96.51	methionine gamma-lyase-like [Benincasa hispida]	[more]
XP_004143943.3	9.2e-37	96.51	methionine gamma-lyase [Cucumis sativus] >KGN49981.1 hypothetical protein Csa_00...	[more]
XP_008437446.1	1.2e-36	97.65	PREDICTED: methionine gamma-lyase-like [Cucumis melo] >KAA0042648.1 methionine g...	[more]
KAG7017043.1	7.8e-36	93.02	Methionine gamma-lyase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6579584.1	7.8e-36	93.02	Methionine gamma-lyase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of HG10009012 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SGU9	1.9e-29	76.19	Methionine gamma-lyase OS=Arabidopsis thaliana OX=3702 GN=MGL PE=1 SV=1	[more]
Q8RDT4	6.3e-04	45.16	L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. nucleatum (strain ATC...	[more]

BLAST of HG10009012 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KQL7	4.5e-37	96.51	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G148520 PE=3 SV=1	[more]
A0A5A7TIP4	5.8e-37	97.65	Methionine gamma-lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3AU15	5.8e-37	97.65	methionine gamma-lyase-like OS=Cucumis melo OX=3656 GN=LOC103482860 PE=3 SV=1	[more]
A0A6J1I2P5	3.5e-34	88.37	methionine gamma-lyase-like OS=Cucurbita maxima OX=3661 GN=LOC111469051 PE=3 SV=...	[more]
A0A6J1E0P6	3.5e-34	89.53	methionine gamma-lyase OS=Momordica charantia OX=3673 GN=LOC111026086 PE=3 SV=1	[more]

BLAST of HG10009012 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G64660.1	1.4e-30	76.19	methionine gamma-lyase	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015422	Pyridoxal phosphate-dependent transferase, small domain	GENE3D	3.90.1150.10	Aspartate Aminotransferase, domain 1	coord: 1..84 e-value: 2.3E-11 score: 46.1
IPR000277	Cys/Met metabolism, pyridoxal phosphate-dependent enzyme	PFAM	PF01053	Cys_Met_Meta_PP	coord: 12..66 e-value: 1.0E-6 score: 27.6
IPR000277	Cys/Met metabolism, pyridoxal phosphate-dependent enzyme	PANTHER	PTHR11808	TRANS-SULFURATION ENZYME FAMILY MEMBER	coord: 1..85
None	No IPR available	PANTHER	PTHR11808:SF76	METHIONINE GAMMA-LYASE-LIKE	coord: 1..85
IPR015424	Pyridoxal phosphate-dependent transferase	SUPERFAMILY	53383	PLP-dependent transferases	coord: 14..81

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10009012.1	HG10009012.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019343	cysteine biosynthetic process via cystathionine
biological_process	GO:0019346	transsulfuration
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004123	cystathionine gamma-lyase activity
molecular_function	GO:0030170	pyridoxal phosphate binding
molecular_function	GO:0003824	catalytic activity