Homology
BLAST of HG10008957 vs. NCBI nr
Match:
XP_038874300.1 (cucumisin-like [Benincasa hispida])
HSP 1 Score: 1208.0 bits (3124), Expect = 0.0e+00
Identity = 604/723 (83.54%), Postives = 644/723 (89.07%), Query Frame = 0
Query: 1 MGSHPKDQVSTPSHHMRMLQEAIGSTFPPHSLLHSYKRSFNGFVAKLTKVEAQKVSEMKG 60
MGSHPK QVST SHHMRMLQEAIGSTF PHSLLHSYKRSFNGFVAKLT+ E +KVSEMK
Sbjct: 35 MGSHPKHQVSTSSHHMRMLQEAIGSTFAPHSLLHSYKRSFNGFVAKLTETEVKKVSEMKD 94
Query: 61 VISIFPNGKVQLHTTRSWDFMGLSQQVNRVPSVESDIIVGVIDTGIWPESPSFLDRGYGP 120
VIS+F N K+QLHTTRSWDFMGL+QQVNRVPSVESDIIVGV DTGIWPESPSFLD GYGP
Sbjct: 95 VISVFLNEKIQLHTTRSWDFMGLTQQVNRVPSVESDIIVGVFDTGIWPESPSFLDHGYGP 154
Query: 121 PPPKWKGSCEVSPDFSCNNKIIGARSYRSDGRYPINDIKGPRDSNGHGTHTASTVAGGLV 180
PPPKWKGSCEVS +FSCNNKIIGARSYR +G+YPINDIKGPRDSNGHGTH ASTVAGGLV
Sbjct: 155 PPPKWKGSCEVSSNFSCNNKIIGARSYRRNGQYPINDIKGPRDSNGHGTHVASTVAGGLV 214
Query: 181 GGASMLGLGSGTARGGVPSARIAAYKVCWSDDCFDADILAAFDDAIADGVDIISISVGSK 240
ASMLGLGSGTARGGVPSARIAAYKVCWSD CF DILAAFDDAIADGVDIIS+S+G +
Sbjct: 215 RQASMLGLGSGTARGGVPSARIAAYKVCWSDTCFSVDILAAFDDAIADGVDIISMSLGPR 274
Query: 241 TPRDNYFNDPIAIGAFHAMKKGILTSTSAGNLGPTSFTVTNFSPWSLSMAASTTDRKFLT 300
P NYF+DPIAIG FHAMK GILTSTSAGN GP+SFT+TNFSPWSL++AASTTDRKF+T
Sbjct: 275 KPIANYFSDPIAIGTFHAMKNGILTSTSAGNDGPSSFTLTNFSPWSLTVAASTTDRKFVT 334
Query: 301 AVQLGDGRKFNGVTINTFDLNGTQYPLVYAGNIPNVTAGFNGSISRIFFFFFFFFFFFFF 360
VQLGDGR+FNGVTINTFDLNGTQYP VYAGN+PNVT GFNGSIS
Sbjct: 335 GVQLGDGRRFNGVTINTFDLNGTQYPFVYAGNVPNVTGGFNGSIS--------------- 394
Query: 361 FWEKRLCSGNTVDRELVKGKIAVCDSILSPTNLGSLVSAVGIIMQDKNPKDRASSFPLPA 420
R C GNTVDRELVKGKIA+CD ++ T LGS+ AVGIIMQDK+PKD SFPLPA
Sbjct: 395 ----RFCLGNTVDRELVKGKIALCDILVPQTLLGSIEGAVGIIMQDKSPKDLTFSFPLPA 454
Query: 421 SHLGTQQGALISSYLNLSRLPTATILKSTEGKHETAPFVASFSSRGPNPITPHILKPDLS 480
SHLGTQ+GALISSYLNL+ LPTATI KS EGKHE APFVASFSSRGPNP TP+ILKPDLS
Sbjct: 455 SHLGTQEGALISSYLNLTSLPTATISKSIEGKHEAAPFVASFSSRGPNPTTPNILKPDLS 514
Query: 481 GPGVEILAAWSPLASPSRAKDDKRELLFNIISGTSMSCPHATAVAAYVKSFHPSWSPAAL 540
GPGVEILAAWSP++SPS AKDD R+LLFNIISGTSM+CPHATAVAAYVKSFHPSWSPAAL
Sbjct: 515 GPGVEILAAWSPISSPSTAKDDNRKLLFNIISGTSMACPHATAVAAYVKSFHPSWSPAAL 574
Query: 541 KSALMTTAYLMRAELNPDAEFAYGSGYINPLGTVNPRLIYNASEIDYIRFLCGEGYNTTL 600
KSALMTTA+ MRAELN DAEFAYGSG+INPLG VNP LIYNASEIDYIRFLCGEGYNTTL
Sbjct: 575 KSALMTTAFPMRAELNKDAEFAYGSGHINPLGAVNPGLIYNASEIDYIRFLCGEGYNTTL 634
Query: 601 LQQISGDNSTCSPTNSDEVFDLNYPSFALFTYISTPFSQIYKRRVTNVGSTNSTYKATIF 660
LQ+I DNSTCSPTNSD+VFDLNYPSFAL T+IST F+Q YKRRVTNVGSTNSTYKAT F
Sbjct: 635 LQRIFEDNSTCSPTNSDQVFDLNYPSFALSTHISTSFNQTYKRRVTNVGSTNSTYKATTF 694
Query: 661 APLGLNITVNPSILSFKALGEELKFELTIEGKISKSISSASLVWDDGVHKVRSPIIVFDS 720
AP G+NI+VNPSILSFKALGEELKFEL IEGKIS+SI SASLVWDDGVHKVRSPIIVFDS
Sbjct: 695 APSGINISVNPSILSFKALGEELKFELIIEGKISRSIESASLVWDDGVHKVRSPIIVFDS 738
Query: 721 DKF 724
D F
Sbjct: 755 DPF 738
BLAST of HG10008957 vs. NCBI nr
Match:
XP_008437519.1 (PREDICTED: cucumisin-like isoform X1 [Cucumis melo])
HSP 1 Score: 1174.8 bits (3038), Expect = 0.0e+00
Identity = 594/727 (81.71%), Postives = 642/727 (88.31%), Query Frame = 0
Query: 1 MGSHPKDQV-STP---SHHMRMLQEAIGSTFPPHSLLHSYKRSFNGFVAKLTKVEAQKVS 60
MGSHPKDQV STP HHMR+LQEAIGSTF PH LLHSYKRSFNGFVAKLT+ EA+KVS
Sbjct: 37 MGSHPKDQVSSTPLSSHHHMRLLQEAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVS 96
Query: 61 EMKGVISIFPNGKVQLHTTRSWDFMGLSQQVNRVPSVESDIIVGVIDTGIWPESPSFLDR 120
EM+GVIS+FPN ++QLHTTRSWDFMG S+QV RVPSVESDIIVGV DTGIWPESPSFLD
Sbjct: 97 EMEGVISVFPNEELQLHTTRSWDFMGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDH 156
Query: 121 GYGPPPPKWKGSCEVSPDFSCNNKIIGARSYRSDGRYPINDIKGPRDSNGHGTHTASTVA 180
GYGPPPPKWKGSCEVS +FSCNNKIIGA+SYRSDGRYPI+DIKGPRDSNGHGTH ASTVA
Sbjct: 157 GYGPPPPKWKGSCEVSLNFSCNNKIIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVA 216
Query: 181 GGLVGGASMLGLGSGTARGGVPSARIAAYKVCWSDDCFDADILAAFDDAIADGVDIISIS 240
GGLV ASMLGLG+GTARGGVPSARIAAYKVCWSD C AD+LAAFDDAIADGVDIIS+S
Sbjct: 217 GGLVRQASMLGLGTGTARGGVPSARIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLS 276
Query: 241 VGSKTPRDNYFNDPIAIGAFHAMKKGILTSTSAGNLGPTSFTVTNFSPWSLSMAASTTDR 300
VG K P NYFNDPIAIG FHAM+ GILTS+SAGN GP FT+TNFSPW+LS+AASTTDR
Sbjct: 277 VGPKRPIPNYFNDPIAIGTFHAMRNGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDR 336
Query: 301 KFLTAVQLGDGRKFNGVTINTFDLNGTQYPLVYAGNIPNVTAGFNGSISRIFFFFFFFFF 360
+FLTAVQLGDGRKFNGVTINTFDLNGTQYPLV+AGNIPNVT GFNGSIS
Sbjct: 337 RFLTAVQLGDGRKFNGVTINTFDLNGTQYPLVHAGNIPNVTGGFNGSIS----------- 396
Query: 361 FFFFFWEKRLCSGNTVDRELVKGKIAVCDSILSPTNLGSLVSAVGIIMQDKNPKDRASSF 420
R C GN+VDRELVKGKIAVCDSI+ P+++GSL SA+GIIMQD +PKD ++F
Sbjct: 397 --------RFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLESALGIIMQDGSPKDLTAAF 456
Query: 421 PLPASHLGTQQGALISSYLNLSRLPTATILKSTEGKHETAPFVASFSSRGPNPITPHILK 480
PLPASHLGTQQ LISSYLNL+R+PTATILKSTE K E AP VASFSSRGPNP TP ILK
Sbjct: 457 PLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPLVASFSSRGPNPTTPDILK 516
Query: 481 PDLSGPGVEILAAWSPLASPSRAKDDKRELLFNIISGTSMSCPHATAVAAYVKSFHPSWS 540
PD+ GPGVEILAAWSP+ SPS AK D R+LLFNIISGTSM+CPHATAVAAYVKSFHPSWS
Sbjct: 517 PDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMACPHATAVAAYVKSFHPSWS 576
Query: 541 PAALKSALMTTAYLMRAELNPDAEFAYGSGYINPLGTVNPRLIYNASEIDYIRFLCGEGY 600
PAALKSAL+TTA+ MR +L P+AEFAYGSG+INPLG VNP LIYNASEIDYIRFLCGEGY
Sbjct: 577 PAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLIYNASEIDYIRFLCGEGY 636
Query: 601 NTTLLQQISGDNSTCSPTNSDEVFDLNYPSFALFTYISTPFSQIYKRRVTNVGSTNSTYK 660
NTTLL+ I+ DNSTCSPTNS V+DLNY SFALFTYISTPFSQ+YKRRVTNVGSTNSTYK
Sbjct: 637 NTTLLRIITKDNSTCSPTNSGLVYDLNYHSFALFTYISTPFSQVYKRRVTNVGSTNSTYK 696
Query: 661 ATIFAPLGLNITVNPSILSFKALGEELKFELTIEGKISKSISSASLVWDDGVHKVRSPII 720
ATIFAP LNITVNPS LSFKAL EELKFE+TIEGKI+ SI+SASLVWDDGVHKVRSPII
Sbjct: 697 ATIFAPSELNITVNPSTLSFKALEEELKFEVTIEGKINGSIASASLVWDDGVHKVRSPII 744
Query: 721 VFDSDKF 724
VFDSD F
Sbjct: 757 VFDSDTF 744
BLAST of HG10008957 vs. NCBI nr
Match:
XP_008437521.1 (PREDICTED: cucumisin-like isoform X2 [Cucumis melo])
HSP 1 Score: 1170.6 bits (3027), Expect = 0.0e+00
Identity = 592/725 (81.66%), Postives = 640/725 (88.28%), Query Frame = 0
Query: 3 SHPKDQV-STP---SHHMRMLQEAIGSTFPPHSLLHSYKRSFNGFVAKLTKVEAQKVSEM 62
SHPKDQV STP HHMR+LQEAIGSTF PH LLHSYKRSFNGFVAKLT+ EA+KVSEM
Sbjct: 6 SHPKDQVSSTPLSSHHHMRLLQEAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEM 65
Query: 63 KGVISIFPNGKVQLHTTRSWDFMGLSQQVNRVPSVESDIIVGVIDTGIWPESPSFLDRGY 122
+GVIS+FPN ++QLHTTRSWDFMG S+QV RVPSVESDIIVGV DTGIWPESPSFLD GY
Sbjct: 66 EGVISVFPNEELQLHTTRSWDFMGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGY 125
Query: 123 GPPPPKWKGSCEVSPDFSCNNKIIGARSYRSDGRYPINDIKGPRDSNGHGTHTASTVAGG 182
GPPPPKWKGSCEVS +FSCNNKIIGA+SYRSDGRYPI+DIKGPRDSNGHGTH ASTVAGG
Sbjct: 126 GPPPPKWKGSCEVSLNFSCNNKIIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGG 185
Query: 183 LVGGASMLGLGSGTARGGVPSARIAAYKVCWSDDCFDADILAAFDDAIADGVDIISISVG 242
LV ASMLGLG+GTARGGVPSARIAAYKVCWSD C AD+LAAFDDAIADGVDIIS+SVG
Sbjct: 186 LVRQASMLGLGTGTARGGVPSARIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVG 245
Query: 243 SKTPRDNYFNDPIAIGAFHAMKKGILTSTSAGNLGPTSFTVTNFSPWSLSMAASTTDRKF 302
K P NYFNDPIAIG FHAM+ GILTS+SAGN GP FT+TNFSPW+LS+AASTTDR+F
Sbjct: 246 PKRPIPNYFNDPIAIGTFHAMRNGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRF 305
Query: 303 LTAVQLGDGRKFNGVTINTFDLNGTQYPLVYAGNIPNVTAGFNGSISRIFFFFFFFFFFF 362
LTAVQLGDGRKFNGVTINTFDLNGTQYPLV+AGNIPNVT GFNGSIS
Sbjct: 306 LTAVQLGDGRKFNGVTINTFDLNGTQYPLVHAGNIPNVTGGFNGSIS------------- 365
Query: 363 FFFWEKRLCSGNTVDRELVKGKIAVCDSILSPTNLGSLVSAVGIIMQDKNPKDRASSFPL 422
R C GN+VDRELVKGKIAVCDSI+ P+++GSL SA+GIIMQD +PKD ++FPL
Sbjct: 366 ------RFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLESALGIIMQDGSPKDLTAAFPL 425
Query: 423 PASHLGTQQGALISSYLNLSRLPTATILKSTEGKHETAPFVASFSSRGPNPITPHILKPD 482
PASHLGTQQ LISSYLNL+R+PTATILKSTE K E AP VASFSSRGPNP TP ILKPD
Sbjct: 426 PASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPLVASFSSRGPNPTTPDILKPD 485
Query: 483 LSGPGVEILAAWSPLASPSRAKDDKRELLFNIISGTSMSCPHATAVAAYVKSFHPSWSPA 542
+ GPGVEILAAWSP+ SPS AK D R+LLFNIISGTSM+CPHATAVAAYVKSFHPSWSPA
Sbjct: 486 VIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMACPHATAVAAYVKSFHPSWSPA 545
Query: 543 ALKSALMTTAYLMRAELNPDAEFAYGSGYINPLGTVNPRLIYNASEIDYIRFLCGEGYNT 602
ALKSAL+TTA+ MR +L P+AEFAYGSG+INPLG VNP LIYNASEIDYIRFLCGEGYNT
Sbjct: 546 ALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLIYNASEIDYIRFLCGEGYNT 605
Query: 603 TLLQQISGDNSTCSPTNSDEVFDLNYPSFALFTYISTPFSQIYKRRVTNVGSTNSTYKAT 662
TLL+ I+ DNSTCSPTNS V+DLNY SFALFTYISTPFSQ+YKRRVTNVGSTNSTYKAT
Sbjct: 606 TLLRIITKDNSTCSPTNSGLVYDLNYHSFALFTYISTPFSQVYKRRVTNVGSTNSTYKAT 665
Query: 663 IFAPLGLNITVNPSILSFKALGEELKFELTIEGKISKSISSASLVWDDGVHKVRSPIIVF 722
IFAP LNITVNPS LSFKAL EELKFE+TIEGKI+ SI+SASLVWDDGVHKVRSPIIVF
Sbjct: 666 IFAPSELNITVNPSTLSFKALEEELKFEVTIEGKINGSIASASLVWDDGVHKVRSPIIVF 711
Query: 723 DSDKF 724
DSD F
Sbjct: 726 DSDTF 711
BLAST of HG10008957 vs. NCBI nr
Match:
XP_023550545.1 (cucumisin-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1159.1 bits (2997), Expect = 0.0e+00
Identity = 578/725 (79.72%), Postives = 632/725 (87.17%), Query Frame = 0
Query: 1 MGSHPKDQVSTPSHHMRMLQEAIGSTFPPHSLLHSYKRSFNGFVAKLTKVEAQKVSEMKG 60
MGSHPKD+VST SHH+RMLQE IGS+F PHSLLHSY+RSFNGFVAKLT+VE QKVSEMKG
Sbjct: 35 MGSHPKDRVSTRSHHVRMLQETIGSSFAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKG 94
Query: 61 VISIFPNGKVQLHTTRSWDFMGLSQQVNRVPSVESDIIVGVIDTGIWPESPSFLDRGYGP 120
VIS+FPNGK QLHTTRSWDFMGLSQQ +RVPSVESDIIVGV+DTGIWPESPSFLD+GYGP
Sbjct: 95 VISVFPNGKKQLHTTRSWDFMGLSQQASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGP 154
Query: 121 PPPKWKGSCEVSPDFSCNNKIIGARSYRSDGRYPINDIKGPRDSNGHGTHTASTVAGGLV 180
PP KWKGSCEVS DFSCNNKIIGARSYR++G+YPINDI+GPRDSNGHGTH ASTVAGGLV
Sbjct: 155 PPRKWKGSCEVSSDFSCNNKIIGARSYRTNGQYPINDIQGPRDSNGHGTHAASTVAGGLV 214
Query: 181 GGASMLGLGSGTARGGVPSARIAAYKVCWSDDCFDADILAAFDDAIADGVDIISISVGSK 240
ASMLGLGSGTARGGVPSARIA+YK+CWSD C DAD+LAAFDDAIADGVDIIS SVG +
Sbjct: 215 RQASMLGLGSGTARGGVPSARIASYKICWSDGCRDADVLAAFDDAIADGVDIISFSVGGR 274
Query: 241 TPRDNYFNDPIAIGAFHAMKKGILTSTSAGNLGPTSFTVTNFSPWSLSMAASTTDRKFLT 300
PRD YFND IAIGAFHAMKKGILTS SAGN GP SFTV NFSPWSLS+AASTTDRKFL+
Sbjct: 275 KPRD-YFNDSIAIGAFHAMKKGILTSMSAGNSGPKSFTVRNFSPWSLSVAASTTDRKFLS 334
Query: 301 AVQLGDGRKFNGVTINTFDLNGTQYPLVYAGNIPNVTAGFNGSISRIFFFFFFFFFFFFF 360
VQLGDGR F+GVTINTFDLNGTQYPLVYAGNIPN+ GFNGSIS
Sbjct: 335 GVQLGDGRSFDGVTINTFDLNGTQYPLVYAGNIPNI--GFNGSIS--------------- 394
Query: 361 FWEKRLCSGNTVDRELVKGKIAVCDSILSPTNLGSLVSAVGIIMQDKNPKDRASSFPLPA 420
R C N+VD+ELVKGKI +CD +SPTNL L A+GIIMQD NPKD FPLPA
Sbjct: 395 ----RFCLTNSVDKELVKGKIVLCDFFVSPTNLSFLEGAIGIIMQDNNPKDLTFPFPLPA 454
Query: 421 SHLGTQQGALISSYLNLSRLPTATILKSTEGKHETAPFVASFSSRGPNPITPHILKPDLS 480
SHLGTQ+GALISSY NL+ LPTATILKSTEGK++ PFVASFSSRGPNPITP ILKPDLS
Sbjct: 455 SHLGTQEGALISSYANLTSLPTATILKSTEGKYKETPFVASFSSRGPNPITPDILKPDLS 514
Query: 481 GPGVEILAAWSPLASPSRAKDDKRELLFNIISGTSMSCPHATAVAAYVKSFHPSWSPAAL 540
GPGVEILAAWSP+ PS A+DD R+LLFNIISGTSMSCPHATAVAAYVKSFHPSWSPAAL
Sbjct: 515 GPGVEILAAWSPIGPPSGAEDDTRQLLFNIISGTSMSCPHATAVAAYVKSFHPSWSPAAL 574
Query: 541 KSALMTTAYLMRAELNPDAEFAYGSGYINPLGTVNPRLIYNASEIDYIRFLCGEGYNTTL 600
KSALMTTA+ MRA+LNPDAEFAYGSG+INPL VNP LIYNA+EIDY+RFLCG+GY+T L
Sbjct: 575 KSALMTTAFPMRADLNPDAEFAYGSGHINPLSAVNPGLIYNATEIDYVRFLCGQGYSTKL 634
Query: 601 LQQISGDNSTCSPTNSDEVFDLNYPSFALFTYISTPFSQIYKRRVTNVGSTNSTYKATIF 660
+QQ+SGDNS+CS + D VFDLNYPSFAL T IST SQ+Y+RRVTNVGS NSTYKA +
Sbjct: 635 VQQVSGDNSSCSRGDIDLVFDLNYPSFALSTSISTSISQVYRRRVTNVGSANSTYKAIVS 694
Query: 661 APLGLNITVNPSILSFKALGEELKFELTIEGKISKSISSASLVWDDGVHKVRSPIIVFDS 720
PLGLNITVNPS+LSFKALGEEL+FE+TIEG IS SI+S SLVWDDG HKV+SPI+VFD
Sbjct: 695 GPLGLNITVNPSVLSFKALGEELRFEVTIEGSISSSIASGSLVWDDGKHKVKSPIVVFDE 737
Query: 721 DKFTN 726
+ F N
Sbjct: 755 NTFIN 737
BLAST of HG10008957 vs. NCBI nr
Match:
KAG6579374.1 (hypothetical protein SDJN03_23822, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1153.3 bits (2982), Expect = 0.0e+00
Identity = 576/725 (79.45%), Postives = 631/725 (87.03%), Query Frame = 0
Query: 1 MGSHPKDQVSTPSHHMRMLQEAIGSTFPPHSLLHSYKRSFNGFVAKLTKVEAQKVSEMKG 60
MGSHPK++V T SHH+RMLQE IGS+F PHSLLHSY+RSFNGFVAKLT+VE QKVSEMKG
Sbjct: 35 MGSHPKERVLTRSHHVRMLQETIGSSFAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKG 94
Query: 61 VISIFPNGKVQLHTTRSWDFMGLSQQVNRVPSVESDIIVGVIDTGIWPESPSFLDRGYGP 120
VIS+F NGK QLHTTRSWDFMGLSQQ +RVPSVESDIIVGV+DTGIWPESPSFLD+GYGP
Sbjct: 95 VISVFLNGKKQLHTTRSWDFMGLSQQASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGP 154
Query: 121 PPPKWKGSCEVSPDFSCNNKIIGARSYRSDGRYPINDIKGPRDSNGHGTHTASTVAGGLV 180
PPPKWKGSCEVS DFSCNNKIIGARSYR++G+YPINDI+GPRDSNGHGTH ASTVAG LV
Sbjct: 155 PPPKWKGSCEVSSDFSCNNKIIGARSYRTNGQYPINDIQGPRDSNGHGTHAASTVAGELV 214
Query: 181 GGASMLGLGSGTARGGVPSARIAAYKVCWSDDCFDADILAAFDDAIADGVDIISISVGSK 240
ASMLGLGSGTARGGVPSARIA+YK+CWSD C DAD+LAAFDDAIADGVDIIS SVG +
Sbjct: 215 RQASMLGLGSGTARGGVPSARIASYKICWSDGCRDADVLAAFDDAIADGVDIISFSVGGR 274
Query: 241 TPRDNYFNDPIAIGAFHAMKKGILTSTSAGNLGPTSFTVTNFSPWSLSMAASTTDRKFLT 300
PRD YFND IAIGAFHAMKKGILTS SAGN GP SFTV NFSPWSLS+AASTTDRK LT
Sbjct: 275 KPRD-YFNDSIAIGAFHAMKKGILTSMSAGNSGPKSFTVRNFSPWSLSVAASTTDRKLLT 334
Query: 301 AVQLGDGRKFNGVTINTFDLNGTQYPLVYAGNIPNVTAGFNGSISRIFFFFFFFFFFFFF 360
VQLGDGR F+GVT+NTFDLNGTQYPLVYAGNIPNV GFNGSIS
Sbjct: 335 GVQLGDGRSFDGVTVNTFDLNGTQYPLVYAGNIPNV--GFNGSIS--------------- 394
Query: 361 FWEKRLCSGNTVDRELVKGKIAVCDSILSPTNLGSLVSAVGIIMQDKNPKDRASSFPLPA 420
R C N+VD+E VKGKI +CD +SPTNL L A+GIIMQD NPKD FPLPA
Sbjct: 395 ----RFCLTNSVDKESVKGKIVLCDFFISPTNLSFLEGAIGIIMQDNNPKDLTFPFPLPA 454
Query: 421 SHLGTQQGALISSYLNLSRLPTATILKSTEGKHETAPFVASFSSRGPNPITPHILKPDLS 480
SHLGTQ+GALISSY NL+ LPTATILKSTEGK++ PFVASFSSRGPNPITP ILKPDLS
Sbjct: 455 SHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRGPNPITPDILKPDLS 514
Query: 481 GPGVEILAAWSPLASPSRAKDDKRELLFNIISGTSMSCPHATAVAAYVKSFHPSWSPAAL 540
GPGVEILAAWSP+ PS A++D R+LLFNIISGTSMSCPHATAVAAYVKSFHPSWSPAAL
Sbjct: 515 GPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYVKSFHPSWSPAAL 574
Query: 541 KSALMTTAYLMRAELNPDAEFAYGSGYINPLGTVNPRLIYNASEIDYIRFLCGEGYNTTL 600
KSALMTTA+ MR++LNPDAEFAYGSG+INPLG VNP LIYNA+EIDY+RFLCG+GY+T L
Sbjct: 575 KSALMTTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNATEIDYVRFLCGQGYSTKL 634
Query: 601 LQQISGDNSTCSPTNSDEVFDLNYPSFALFTYISTPFSQIYKRRVTNVGSTNSTYKATIF 660
+QQ+SGDNS+CS ++SD VFDLNYPSFAL T IST SQ+Y+R+VTNVGS NS YKAT+
Sbjct: 635 IQQVSGDNSSCSRSDSDVVFDLNYPSFALSTPISTSISQVYRRKVTNVGSANSIYKATVS 694
Query: 661 APLGLNITVNPSILSFKALGEELKFELTIEGKISKSISSASLVWDDGVHKVRSPIIVFDS 720
P GL ITVNPS+LSFKALGEEL FE+TIEG IS SI+SASLVWDDG HKVRSPIIVFDS
Sbjct: 695 GPSGLKITVNPSVLSFKALGEELSFEVTIEGSISSSIASASLVWDDGQHKVRSPIIVFDS 737
Query: 721 DKFTN 726
+KF N
Sbjct: 755 NKFIN 737
BLAST of HG10008957 vs. ExPASy Swiss-Prot
Match:
Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)
HSP 1 Score: 772.3 bits (1993), Expect = 4.8e-222
Identity = 402/718 (55.99%), Postives = 496/718 (69.08%), Query Frame = 0
Query: 1 MGSHPKDQVSTPSHHMRMLQEAIGSTFPPHSLLHSYKRSFNGFVAKLTKVEAQKVSEMKG 60
MG +D S HH ML++ +GSTF P S+LH+YKRSFNGF KLT+ EA+K++ M+G
Sbjct: 38 MGRKLEDPDSAHLHHRAMLEQVVGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEG 97
Query: 61 VISIFPNGKVQLHTTRSWDFMGLSQQVNRVPSVESDIIVGVIDTGIWPESPSFLDRGYGP 120
V+S+F N +LHTTRSWDF+G V R VES+I+VGV+DTGIWPESPSF D G+ P
Sbjct: 98 VVSVFLNEMNELHTTRSWDFLGFPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSP 157
Query: 121 PPPKWKGSCEVSPDFSCNNKIIGARSYRSDGRYPINDIKGPRDSNGHGTHTASTVAGGLV 180
PPPKWKG+CE S +F CN KIIGARSY D+ GPRD+NGHGTHTAST AGGLV
Sbjct: 158 PPPKWKGTCETSNNFRCNRKIIGARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLV 217
Query: 181 GGASMLGLGSGTARGGVPSARIAAYKVCWSDDCFDADILAAFDDAIADGVDIISISVGSK 240
A++ GLG GTARGGVP ARIAAYKVCW+D C D DILAA+DDAIADGVDIIS+SVG
Sbjct: 218 SQANLYGLGLGTARGGVPLARIAAYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGA 277
Query: 241 TPRDNYFNDPIAIGAFHAMKKGILTSTSAGNLGPTSFTVTNFSPWSLSMAASTTDRKFLT 300
PR +YF D IAIG+FHA+++GILTS SAGN GP FT + SPW LS+AAST DRKF+T
Sbjct: 278 NPR-HYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVT 337
Query: 301 AVQLGDGRKFNGVTINTFDLNGTQYPLVYAGNIPNVTAGFNGSISRIFFFFFFFFFFFFF 360
VQ+G+G+ F GV+INTFD YPLV +IPN GF+ S S
Sbjct: 338 QVQIGNGQSFQGVSINTFD--NQYYPLVSGRDIPN--TGFDKSTS--------------- 397
Query: 361 FWEKRLCSGNTVDRELVKGKIAVCDSILSPTN-LGSLVSAVGIIMQDKNPKDRASSFPLP 420
R C+ +V+ L+KGKI VC++ P SL A G++M N +D A S+PLP
Sbjct: 398 ----RFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVLM-TSNTRDYADSYPLP 457
Query: 421 ASHLGTQQGALISSYLNLSRLPTATILKSTEGKHETAPFVASFSSRGPNPITPHILKPDL 480
+S L Y+ R P ATI KST + +AP V SFSSRGPN T ++KPD+
Sbjct: 458 SSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDI 517
Query: 481 SGPGVEILAAWSPLASPSRAKDDKRELLFNIISGTSMSCPHATAVAAYVKSFHPSWSPAA 540
SGPGVEILAAW +A +R LFNIISGTSMSCPH T +A YVK+++P+WSPAA
Sbjct: 518 SGPGVEILAAWPSVAPVGGI---RRNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAA 577
Query: 541 LKSALMTTAYLMRAELNPDAEFAYGSGYINPLGTVNPRLIYNASEIDYIRFLCGEGYNTT 600
+KSALMTTA M A NP AEFAYGSG++NPL V P L+Y+A+E DY++FLCG+GYNT
Sbjct: 578 IKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQ 637
Query: 601 LLQQISGDNSTCSPTNSDEVFDLNYPSFALFTYISTPFSQIYKRRVTNVGSTNSTYKATI 660
+++I+GD S C+ N+ V+DLNYPSF L S F+Q + R +T+V STY+A I
Sbjct: 638 AVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQYFNRTLTSVAPQASTYRAMI 697
Query: 661 FAPLGLNITVNPSILSFKALGEELKFELTIEGKISKSISSASLVWDDGVHKVRSPIIV 718
AP GL I+VNP++LSF LG+ F LT+ G I + SASLVW DGVH VRSPI +
Sbjct: 698 SAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDGVHYVRSPITI 727
BLAST of HG10008957 vs. ExPASy Swiss-Prot
Match:
Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)
HSP 1 Score: 668.3 bits (1723), Expect = 9.8e-191
Identity = 365/724 (50.41%), Postives = 470/724 (64.92%), Query Frame = 0
Query: 1 MGSHPKDQVSTPSHHMRMLQEAIGSTFPPHSLLHSYKRSFNGFVAKLTKVEAQKVSEMKG 60
MG+ P+ + S PSHH+ +LQ+ +G+ H L+ SYKRSFNGF A L++ E+QK+ MK
Sbjct: 37 MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 96
Query: 61 VISIFPNGKVQLHTTRSWDFMGLSQQVNRVPSVESDIIVGVIDTGIWPESPSFLDRGYGP 120
V+S+FP+ +L TTRSWDF+G ++ R ESD+IVGVID+GIWPES SF D G+GP
Sbjct: 97 VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 156
Query: 121 PPPKWKGSCEVSPDFSCNNKIIGARSYRSDGRYPINDIKGPRDSNGHGTHTASTVAGGLV 180
PP KWKGSC+ F+CNNK+IGAR Y RD GHGTHTAST AG V
Sbjct: 157 PPKKWKGSCKGGLKFACNNKLIGARFYNKFA-------DSARDEEGHGTHTASTAAGNAV 216
Query: 181 GGASMLGLGSGTARGGVPSARIAAYKVCWSDDCFDADILAAFDDAIADGVDIISISVGSK 240
AS GL GTARGGVPSARIAAYKVC+ + C D DILAAFDDAIADGVD+ISIS+ S
Sbjct: 217 QAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISI-SA 276
Query: 241 TPRDNYFNDPIAIGAFHAMKKGILTSTSAGNLGPTSFTVTNFSPWSLSMAASTTDRKFLT 300
N N +AIG+FHAM +GI+T+ SAGN GP +V N SPW +++AAS TDR+F+
Sbjct: 277 DYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFID 336
Query: 301 AVQLGDGRKFNGVTINTFDLNGTQYPLVYAGNIPNVTAGFNGSISRIFFFFFFFFFFFFF 360
V LG+G+ G+++NTF+LNGT++P+VY N+ N S ++ +
Sbjct: 337 RVVLGNGKALTGISVNTFNLNGTKFPIVYGQNVSR-----NCSQAQAGY----------- 396
Query: 361 FWEKRLCSGNTVDRELVKGKIAVCDSILSPTNLGSLVSAVGIIMQDKNPKDRASSFPLPA 420
CS VD ELVKGKI +CD L L A+G+I+Q+ D A P PA
Sbjct: 397 ------CSSGCVDSELVKGKIVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPA 456
Query: 421 SHLGTQQGALISSYLNLSRLPTATILKSTEGKHETAPFVASFSSRGPNPITPHILKPDLS 480
S LG + I SY+ + P A IL++ E AP+V SFSSRGP+ + ++LKPD+S
Sbjct: 457 SSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVS 516
Query: 481 GPGVEILAAWSPLASPSR--AKDDKRELLFNIISGTSMSCPHATAVAAYVKSFHPSWSPA 540
PG+EILAA+SP+ASPS +DKR + ++++SGTSM+CPH VAAYVKSFHP WSP+
Sbjct: 517 APGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPS 576
Query: 541 ALKSALMTTAYLMRAELNPDAEFAYGSGYINPLGTVNPRLIYNASEIDYIRFLCGEGYNT 600
A+KSA+MTTA M + NP+ EFAYGSG INP +P L+Y DY++ LC EG+++
Sbjct: 577 AIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDS 636
Query: 601 TLLQQISGDNSTCSPTNSDEVFDLNYPSFALFTYISTPFSQIYKRRVTNVGSTNSTYKAT 660
T L SG N TCS EV DLNYP+ F PF+ +KR VTNVG NSTYKA+
Sbjct: 637 TTLTTTSGQNVTCS--ERTEVKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKAS 696
Query: 661 IFAPL--GLNITVNPSILSFKALGEELKFELTIEGKISK--SISSASLVWDDGVHKVRSP 719
+ PL L I++ P IL F L E+ F +TI GK K S S+S+VW DG H VRSP
Sbjct: 697 V-VPLQPELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSP 725
BLAST of HG10008957 vs. ExPASy Swiss-Prot
Match:
Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)
HSP 1 Score: 628.2 bits (1619), Expect = 1.1e-178
Identity = 348/731 (47.61%), Postives = 464/731 (63.47%), Query Frame = 0
Query: 1 MGSHPKDQVSTP-SHHMRMLQEAIGSTFPPHSLLHSYKRSFNGFVAKLTKVEAQKVSEMK 60
MGS TP S HM +LQE G + L+ SYKRSFNGF A+LT+ E ++V++M
Sbjct: 36 MGSLSSRADYTPTSDHMNILQEVTGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMV 95
Query: 61 GVISIFPNGKVQLHTTRSWDFMGLSQ--QVNRVPSVESDIIVGVIDTGIWPESPSFLDRG 120
GV+S+FPN K+QL TT SWDFMGL + + R P+VESD I+GVID+GI PES SF D+G
Sbjct: 96 GVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKG 155
Query: 121 YGPPPPKWKGSCEVSPDFSCNNKIIGARSYRSDGRYPINDIKGPRDSNGHGTHTASTVAG 180
+GPPP KWKG C +F+CNNK+IGAR Y S+ G RD +GHGTHTAST AG
Sbjct: 156 FGPPPQKWKGVCSGGKNFTCNNKLIGARDYTSE---------GTRDMDGHGTHTASTAAG 215
Query: 181 GLVGGASMLGLGSGTARGGVPSARIAAYKVCWSDDCFDADILAAFDDAIADGVDIISISV 240
V AS G+G+GT RGGVP++R+AAYKVC C +L+AFDDAIADGVD+I+IS+
Sbjct: 216 NAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISI 275
Query: 241 GSKTPRDNYFNDPIAIGAFHAMKKGILTSTSAGNLGPTSFTVTNFSPWSLSMAASTTDRK 300
G KT + NDPIAIGAFHAM KG+LT SAGN GP +V+ +PW L++AASTT+R
Sbjct: 276 GDKT-ASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRG 335
Query: 301 FLTAVQLGDGRKFNGVTINTFDLNGTQYPLVYAGNIPNVTAGFNGSISRIFFFFFFFFFF 360
F+T V LG+G+ G ++N +++ G YPLVY + + +
Sbjct: 336 FVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSAASSACDAESA-------------- 395
Query: 361 FFFFWEKRLCSGNTVDRELVKGKIAVCDSILSPTNLGSLVSAVGIIMQDKNPKDRASSFP 420
LC + VD+ VKGKI VC + V AVG+I + P D A P
Sbjct: 396 -------GLCELSCVDKSRVKGKILVCGG-PGGLKIVESVGAVGLIYRTPKP-DVAFIHP 455
Query: 421 LPASHLGTQQGALISSYLNLSRLPTATILKSTEGKHETAPFVASFSSRGPNPITPHILKP 480
LPA+ L T+ + SYL + P A +LK+ + T+P +ASFSSRGPN I ILKP
Sbjct: 456 LPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKP 515
Query: 481 DLSGPGVEILAAWSPLASPSRAKDDKRELLFNIISGTSMSCPHATAVAAYVKSFHPSWSP 540
D++ PGVEILAA+SP PS +DD R + ++++SGTSMSCPH VAAYVK+F+P WSP
Sbjct: 516 DITAPGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSP 575
Query: 541 AALKSALMTTAYLMRAELN--PDAEFAYGSGYINPLGTVNPRLIYNASEIDYIRFLCGEG 600
+ ++SA+MTTA+ + A EFAYGSG+++P+ NP L+Y + D+I FLCG
Sbjct: 576 SMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMN 635
Query: 601 YNTTLLQQISGDNSTCSPTNSDEVFDLNYPSF-ALFTYISTPFSQIYKRRVTNVGSTNST 660
Y + +L+ ISG+ TCS +LNYPS A + T F+ + R +TNVG+ NST
Sbjct: 636 YTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNST 695
Query: 661 YKATIFAPLG--LNITVNPSILSFKALGEELKFELTIEGK--ISKSISSASLVWDDGVHK 720
Y + + A G L++ + PS+LSFK + E+ F +T+ G S+ SSA+L+W DG H
Sbjct: 696 YTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSDGTHN 731
Query: 721 VRSPIIVFDSD 722
VRSPI+V+ SD
Sbjct: 756 VRSPIVVYTSD 731
BLAST of HG10008957 vs. ExPASy Swiss-Prot
Match:
Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)
HSP 1 Score: 622.5 bits (1604), Expect = 6.1e-177
Identity = 343/734 (46.73%), Postives = 475/734 (64.71%), Query Frame = 0
Query: 1 MGSHPKDQVSTP-SHHMRMLQEAIGSTFPPHSLLHSYKRSFNGFVAKLTKVEAQKVSEMK 60
+GS P + TP S HM +LQE G + + L+ SYK+SFNGF A+LT+ E ++++ M+
Sbjct: 39 LGSLPSREEYTPMSDHMSILQEITGESLIENRLVRSYKKSFNGFAARLTESERKRLAGME 98
Query: 61 GVISIFPNGKVQLHTTRSWDFMGLSQ--QVNRVPSVESDIIVGVIDTGIWPESPSFLDRG 120
V+S+FP+ K++L TT SW+FMGL + + R S+ESD I+GVID+GI+PES SF D+G
Sbjct: 99 RVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQG 158
Query: 121 YGPPPPKWKGSCEVSPDFSCNNKIIGARSYRSDGRYPINDIKGPRDSNGHGTHTASTVAG 180
+GPPP KWKG+C +F+CNNK+IGAR Y + + + RD +GHGTHTAS AG
Sbjct: 159 FGPPPKKWKGTCAGGKNFTCNNKVIGARDYTAKSKAN----QTARDYSGHGTHTASIAAG 218
Query: 181 GLVGGASMLGLGSGTARGGVPSARIAAYKVCWSDDCFDADILAAFDDAIADGVDIISISV 240
V ++ GLG+GTARGGVP+ARIA YKVC ++ C +++AFDDAIADGVD+ISIS+
Sbjct: 219 NAVANSNFYGLGNGTARGGVPAARIAVYKVCDNEGCDGEAMMSAFDDAIADGVDVISISI 278
Query: 241 GSKTPRDN---YFNDPIAIGAFHAMKKGILTSTSAGNLGPTSFTVTNFSPWSLSMAASTT 300
DN + DPIAIGAFHAM G+LT +AGN GP TVT+ +PW S+AAS T
Sbjct: 279 ----VLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGPKISTVTSTAPWVFSVAASVT 338
Query: 301 DRKFLTAVQLGDGRKFNGVTINTFDLNGTQYPLVYAGNIPNVTAGFNGSISRIFFFFFFF 360
+R F+ V LGDG+ G ++NT+D+NGT YPLVY + T +
Sbjct: 339 NRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSAALSTCSVD------------- 398
Query: 361 FFFFFFFWEKRLCSGNTVDRELVKGKIAVCDS---ILSPTNLGSLVSAVGIIMQDKNPKD 420
+ RLC +D +LVKGKI +CDS ++ LG AVG I+++ P D
Sbjct: 399 --------KARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLG----AVGSIVKNPEP-D 458
Query: 421 RASSFPLPASHLGTQQGALISSYLNLSRLPTATILKSTEGKHETAPFVASFSSRGPNPIT 480
RA P S L + SY+N ++ P AT+LKS E ++ AP VASFSSRGP+ I
Sbjct: 459 RAFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIV 518
Query: 481 PHILKPDLSGPGVEILAAWSPLASPSRAKDDKRELLFNIISGTSMSCPHATAVAAYVKSF 540
ILKPD++ PGVEILAA+SP +SP+ ++ D R + ++++SGTSM+CPH VAAYVK+F
Sbjct: 519 SDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTF 578
Query: 541 HPSWSPAALKSALMTTAYLMRAELN--PDAEFAYGSGYINPLGTVNPRLIYNASEIDYIR 600
HP WSP+ ++SA+MTTA+ M A + EFAYGSG+++P+ +NP L+Y ++ D+I
Sbjct: 579 HPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHIN 638
Query: 601 FLCGEGYNTTLLQQISGDNSTCSPTNSDEV-FDLNYPSFALFTYISTPFSQIYKRRVTNV 660
FLCG Y + L+ ISGDNSTC+ S + +LNYP+ + + PF+ ++R VTNV
Sbjct: 639 FLCGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQRTVTNV 698
Query: 661 GSTNSTYKATI--FAPLGLNITVNPSILSFKALGEELKFELTIEGKI--SKSISSASLVW 719
G STY A + F L+I V+P +LS K++ E+ F +T+ +K SA+L+W
Sbjct: 699 GMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQPVSANLIW 738
BLAST of HG10008957 vs. ExPASy Swiss-Prot
Match:
Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)
HSP 1 Score: 615.9 bits (1587), Expect = 5.7e-175
Identity = 338/716 (47.21%), Postives = 460/716 (64.25%), Query Frame = 0
Query: 13 SHHMRMLQEAIGSTFPPHSLLHSYKRSFNGFVAKLTKVEAQKVSEMKGVISIFPNGKVQL 72
S HM +LQ+ G + L+ SYKRSFNGF A+LT+ E ++E++GV+S+FPN +QL
Sbjct: 50 SDHMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQL 109
Query: 73 HTTRSWDFMGLSQQVN--RVPSVESDIIVGVIDTGIWPESPSFLDRGYGPPPPKWKGSCE 132
HTT SWDFMG+ + N R ++ESD I+GVIDTGIWPES SF D+G+GPPP KWKG C
Sbjct: 110 HTTTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCS 169
Query: 133 VSPDFSCNNKIIGARSYRSDGRYPINDIKGPRDSNGHGTHTASTVAGGLVGGASMLGLGS 192
+F+CNNK+IGAR Y S+ G RD++GHGTHTAST AG V S G+G+
Sbjct: 170 GGKNFTCNNKLIGARDYTSE---------GTRDTSGHGTHTASTAAGNAVKDTSFFGIGN 229
Query: 193 GTARGGVPSARIAAYKVCWSDDCFDADILAAFDDAIADGVDIISISVGSKTPRDNYFNDP 252
GT RGGVP++RIAAYKVC C +L++FDDAIADGVD+I+IS+G + P + +DP
Sbjct: 230 GTVRGGVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFP-SIFEDDP 289
Query: 253 IAIGAFHAMKKGILTSTSAGNLGPTSFTVTNFSPWSLSMAASTTDRKFLTAVQLGDGRKF 312
IAIGAFHAM KGILT +SAGN GP TV++ +PW ++AASTT+R F+T V LG+G+
Sbjct: 290 IAIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTL 349
Query: 313 NGVTINTFDLNGTQYPLVYAGNIPNVTAGFNGSISRIFFFFFFFFFFFFFFWEKRLCSGN 372
G ++N FD+ G +YPLVY + + + LC+
Sbjct: 350 AGRSVNAFDMKGKKYPLVYGKSAASSACDAKTA---------------------ALCAPA 409
Query: 373 TVDRELVKGKIAVCDSILSPTNLGSLVSAVGIIMQDKNPK-DRASSFPLPASHLGTQQGA 432
+++ VKGKI VC S + V A+ II DK+P+ D A + LPAS L +
Sbjct: 410 CLNKSRVKGKILVCGG-PSGYKIAKSVGAIAII--DKSPRPDVAFTHHLPASGLKAKDFK 469
Query: 433 LISSYLNLSRLPTATILKSTEGKHETAPFVASFSSRGPNPITPHILKPDLSGPGVEILAA 492
+ SY+ P A +LK+ + T+P +ASFSSRGPN I ILKPD++ PGVEILAA
Sbjct: 470 SLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAA 529
Query: 493 WSPLASPSRAKDDKRELLFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALMTTAY 552
+SP PS +DD R + +++ SGTSM+CPH VAAYVK+F+P WSP+ ++SA+MTTA+
Sbjct: 530 FSPNGEPS--EDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAW 589
Query: 553 LMRAELN--PDAEFAYGSGYINPLGTVNPRLIYNASEIDYIRFLCGEGYNTTLLQQISGD 612
++A+ EFAYG+G+++P+ +NP L+Y + D+I FLCG Y + L+ ISGD
Sbjct: 590 PVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGD 649
Query: 613 NSTCSPTNSDEVFDLNYPSF-ALFTYISTPFSQIYKRRVTNVGSTNSTYKATIFAPLG-- 672
CS N +LNYPS A + + FS + R +TNVG+ NSTYK+ + A G
Sbjct: 650 TVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSK 709
Query: 673 LNITVNPSILSFKALGEELKFELTIEGK--ISKSISSASLVWDDGVHKVRSPIIVF 719
L+I V PS+L FK + E+ F +T+ G S+ SSA+L+W DG H VRSPI+V+
Sbjct: 710 LSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIVVY 729
BLAST of HG10008957 vs. ExPASy TrEMBL
Match:
A0A1S3AUC4 (cucumisin-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482910 PE=3 SV=1)
HSP 1 Score: 1174.8 bits (3038), Expect = 0.0e+00
Identity = 594/727 (81.71%), Postives = 642/727 (88.31%), Query Frame = 0
Query: 1 MGSHPKDQV-STP---SHHMRMLQEAIGSTFPPHSLLHSYKRSFNGFVAKLTKVEAQKVS 60
MGSHPKDQV STP HHMR+LQEAIGSTF PH LLHSYKRSFNGFVAKLT+ EA+KVS
Sbjct: 37 MGSHPKDQVSSTPLSSHHHMRLLQEAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVS 96
Query: 61 EMKGVISIFPNGKVQLHTTRSWDFMGLSQQVNRVPSVESDIIVGVIDTGIWPESPSFLDR 120
EM+GVIS+FPN ++QLHTTRSWDFMG S+QV RVPSVESDIIVGV DTGIWPESPSFLD
Sbjct: 97 EMEGVISVFPNEELQLHTTRSWDFMGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDH 156
Query: 121 GYGPPPPKWKGSCEVSPDFSCNNKIIGARSYRSDGRYPINDIKGPRDSNGHGTHTASTVA 180
GYGPPPPKWKGSCEVS +FSCNNKIIGA+SYRSDGRYPI+DIKGPRDSNGHGTH ASTVA
Sbjct: 157 GYGPPPPKWKGSCEVSLNFSCNNKIIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVA 216
Query: 181 GGLVGGASMLGLGSGTARGGVPSARIAAYKVCWSDDCFDADILAAFDDAIADGVDIISIS 240
GGLV ASMLGLG+GTARGGVPSARIAAYKVCWSD C AD+LAAFDDAIADGVDIIS+S
Sbjct: 217 GGLVRQASMLGLGTGTARGGVPSARIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLS 276
Query: 241 VGSKTPRDNYFNDPIAIGAFHAMKKGILTSTSAGNLGPTSFTVTNFSPWSLSMAASTTDR 300
VG K P NYFNDPIAIG FHAM+ GILTS+SAGN GP FT+TNFSPW+LS+AASTTDR
Sbjct: 277 VGPKRPIPNYFNDPIAIGTFHAMRNGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDR 336
Query: 301 KFLTAVQLGDGRKFNGVTINTFDLNGTQYPLVYAGNIPNVTAGFNGSISRIFFFFFFFFF 360
+FLTAVQLGDGRKFNGVTINTFDLNGTQYPLV+AGNIPNVT GFNGSIS
Sbjct: 337 RFLTAVQLGDGRKFNGVTINTFDLNGTQYPLVHAGNIPNVTGGFNGSIS----------- 396
Query: 361 FFFFFWEKRLCSGNTVDRELVKGKIAVCDSILSPTNLGSLVSAVGIIMQDKNPKDRASSF 420
R C GN+VDRELVKGKIAVCDSI+ P+++GSL SA+GIIMQD +PKD ++F
Sbjct: 397 --------RFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLESALGIIMQDGSPKDLTAAF 456
Query: 421 PLPASHLGTQQGALISSYLNLSRLPTATILKSTEGKHETAPFVASFSSRGPNPITPHILK 480
PLPASHLGTQQ LISSYLNL+R+PTATILKSTE K E AP VASFSSRGPNP TP ILK
Sbjct: 457 PLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPLVASFSSRGPNPTTPDILK 516
Query: 481 PDLSGPGVEILAAWSPLASPSRAKDDKRELLFNIISGTSMSCPHATAVAAYVKSFHPSWS 540
PD+ GPGVEILAAWSP+ SPS AK D R+LLFNIISGTSM+CPHATAVAAYVKSFHPSWS
Sbjct: 517 PDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMACPHATAVAAYVKSFHPSWS 576
Query: 541 PAALKSALMTTAYLMRAELNPDAEFAYGSGYINPLGTVNPRLIYNASEIDYIRFLCGEGY 600
PAALKSAL+TTA+ MR +L P+AEFAYGSG+INPLG VNP LIYNASEIDYIRFLCGEGY
Sbjct: 577 PAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLIYNASEIDYIRFLCGEGY 636
Query: 601 NTTLLQQISGDNSTCSPTNSDEVFDLNYPSFALFTYISTPFSQIYKRRVTNVGSTNSTYK 660
NTTLL+ I+ DNSTCSPTNS V+DLNY SFALFTYISTPFSQ+YKRRVTNVGSTNSTYK
Sbjct: 637 NTTLLRIITKDNSTCSPTNSGLVYDLNYHSFALFTYISTPFSQVYKRRVTNVGSTNSTYK 696
Query: 661 ATIFAPLGLNITVNPSILSFKALGEELKFELTIEGKISKSISSASLVWDDGVHKVRSPII 720
ATIFAP LNITVNPS LSFKAL EELKFE+TIEGKI+ SI+SASLVWDDGVHKVRSPII
Sbjct: 697 ATIFAPSELNITVNPSTLSFKALEEELKFEVTIEGKINGSIASASLVWDDGVHKVRSPII 744
Query: 721 VFDSDKF 724
VFDSD F
Sbjct: 757 VFDSDTF 744
BLAST of HG10008957 vs. ExPASy TrEMBL
Match:
A0A1S3AUU4 (cucumisin-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103482910 PE=3 SV=1)
HSP 1 Score: 1170.6 bits (3027), Expect = 0.0e+00
Identity = 592/725 (81.66%), Postives = 640/725 (88.28%), Query Frame = 0
Query: 3 SHPKDQV-STP---SHHMRMLQEAIGSTFPPHSLLHSYKRSFNGFVAKLTKVEAQKVSEM 62
SHPKDQV STP HHMR+LQEAIGSTF PH LLHSYKRSFNGFVAKLT+ EA+KVSEM
Sbjct: 6 SHPKDQVSSTPLSSHHHMRLLQEAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEM 65
Query: 63 KGVISIFPNGKVQLHTTRSWDFMGLSQQVNRVPSVESDIIVGVIDTGIWPESPSFLDRGY 122
+GVIS+FPN ++QLHTTRSWDFMG S+QV RVPSVESDIIVGV DTGIWPESPSFLD GY
Sbjct: 66 EGVISVFPNEELQLHTTRSWDFMGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGY 125
Query: 123 GPPPPKWKGSCEVSPDFSCNNKIIGARSYRSDGRYPINDIKGPRDSNGHGTHTASTVAGG 182
GPPPPKWKGSCEVS +FSCNNKIIGA+SYRSDGRYPI+DIKGPRDSNGHGTH ASTVAGG
Sbjct: 126 GPPPPKWKGSCEVSLNFSCNNKIIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGG 185
Query: 183 LVGGASMLGLGSGTARGGVPSARIAAYKVCWSDDCFDADILAAFDDAIADGVDIISISVG 242
LV ASMLGLG+GTARGGVPSARIAAYKVCWSD C AD+LAAFDDAIADGVDIIS+SVG
Sbjct: 186 LVRQASMLGLGTGTARGGVPSARIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVG 245
Query: 243 SKTPRDNYFNDPIAIGAFHAMKKGILTSTSAGNLGPTSFTVTNFSPWSLSMAASTTDRKF 302
K P NYFNDPIAIG FHAM+ GILTS+SAGN GP FT+TNFSPW+LS+AASTTDR+F
Sbjct: 246 PKRPIPNYFNDPIAIGTFHAMRNGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRF 305
Query: 303 LTAVQLGDGRKFNGVTINTFDLNGTQYPLVYAGNIPNVTAGFNGSISRIFFFFFFFFFFF 362
LTAVQLGDGRKFNGVTINTFDLNGTQYPLV+AGNIPNVT GFNGSIS
Sbjct: 306 LTAVQLGDGRKFNGVTINTFDLNGTQYPLVHAGNIPNVTGGFNGSIS------------- 365
Query: 363 FFFWEKRLCSGNTVDRELVKGKIAVCDSILSPTNLGSLVSAVGIIMQDKNPKDRASSFPL 422
R C GN+VDRELVKGKIAVCDSI+ P+++GSL SA+GIIMQD +PKD ++FPL
Sbjct: 366 ------RFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLESALGIIMQDGSPKDLTAAFPL 425
Query: 423 PASHLGTQQGALISSYLNLSRLPTATILKSTEGKHETAPFVASFSSRGPNPITPHILKPD 482
PASHLGTQQ LISSYLNL+R+PTATILKSTE K E AP VASFSSRGPNP TP ILKPD
Sbjct: 426 PASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPLVASFSSRGPNPTTPDILKPD 485
Query: 483 LSGPGVEILAAWSPLASPSRAKDDKRELLFNIISGTSMSCPHATAVAAYVKSFHPSWSPA 542
+ GPGVEILAAWSP+ SPS AK D R+LLFNIISGTSM+CPHATAVAAYVKSFHPSWSPA
Sbjct: 486 VIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMACPHATAVAAYVKSFHPSWSPA 545
Query: 543 ALKSALMTTAYLMRAELNPDAEFAYGSGYINPLGTVNPRLIYNASEIDYIRFLCGEGYNT 602
ALKSAL+TTA+ MR +L P+AEFAYGSG+INPLG VNP LIYNASEIDYIRFLCGEGYNT
Sbjct: 546 ALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLIYNASEIDYIRFLCGEGYNT 605
Query: 603 TLLQQISGDNSTCSPTNSDEVFDLNYPSFALFTYISTPFSQIYKRRVTNVGSTNSTYKAT 662
TLL+ I+ DNSTCSPTNS V+DLNY SFALFTYISTPFSQ+YKRRVTNVGSTNSTYKAT
Sbjct: 606 TLLRIITKDNSTCSPTNSGLVYDLNYHSFALFTYISTPFSQVYKRRVTNVGSTNSTYKAT 665
Query: 663 IFAPLGLNITVNPSILSFKALGEELKFELTIEGKISKSISSASLVWDDGVHKVRSPIIVF 722
IFAP LNITVNPS LSFKAL EELKFE+TIEGKI+ SI+SASLVWDDGVHKVRSPIIVF
Sbjct: 666 IFAPSELNITVNPSTLSFKALEEELKFEVTIEGKINGSIASASLVWDDGVHKVRSPIIVF 711
Query: 723 DSDKF 724
DSD F
Sbjct: 726 DSDTF 711
BLAST of HG10008957 vs. ExPASy TrEMBL
Match:
A0A6J1E2G4 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430231 PE=3 SV=1)
HSP 1 Score: 1150.2 bits (2974), Expect = 0.0e+00
Identity = 570/725 (78.62%), Postives = 628/725 (86.62%), Query Frame = 0
Query: 1 MGSHPKDQVSTPSHHMRMLQEAIGSTFPPHSLLHSYKRSFNGFVAKLTKVEAQKVSEMKG 60
MGSHPKD+VST SHH+RMLQE IGS+F PHSLLHSY+RSFNGFV KLT+ E + +SEMKG
Sbjct: 35 MGSHPKDRVSTRSHHVRMLQETIGSSFAPHSLLHSYQRSFNGFVVKLTEDEVKIISEMKG 94
Query: 61 VISIFPNGKVQLHTTRSWDFMGLSQQVNRVPSVESDIIVGVIDTGIWPESPSFLDRGYGP 120
VIS+FPN K QLHTTRSWDFMGLSQQV RVPSVESDIIVGV+DTGIWPESPSFLD GYGP
Sbjct: 95 VISVFPNEKKQLHTTRSWDFMGLSQQVGRVPSVESDIIVGVLDTGIWPESPSFLDEGYGP 154
Query: 121 PPPKWKGSCEVSPDFSCNNKIIGARSYRSDGRYPINDIKGPRDSNGHGTHTASTVAGGLV 180
PPP+WKGSCE S +FSCNNKIIGARSYR++G+YPINDI+GPRDSNGHGTHTASTVAGGLV
Sbjct: 155 PPPRWKGSCEASLNFSCNNKIIGARSYRTNGQYPINDIQGPRDSNGHGTHTASTVAGGLV 214
Query: 181 GGASMLGLGSGTARGGVPSARIAAYKVCWSDDCFDADILAAFDDAIADGVDIISISVGSK 240
ASMLGLGSGTARGGVPSARIA+YK+CWSDDC DADILAAFDDAIADGVDIIS SVG
Sbjct: 215 RQASMLGLGSGTARGGVPSARIASYKICWSDDCSDADILAAFDDAIADGVDIISFSVGGH 274
Query: 241 TPRDNYFNDPIAIGAFHAMKKGILTSTSAGNLGPTSFTVTNFSPWSLSMAASTTDRKFLT 300
TP+D YFND +AIGAFHAMKKGILTS SAGN GP SFT+ NFSPWSLS+AASTT+R +L+
Sbjct: 275 TPKD-YFNDSMAIGAFHAMKKGILTSMSAGNDGPQSFTIRNFSPWSLSVAASTTNRSYLS 334
Query: 301 AVQLGDGRKFNGVTINTFDLNGTQYPLVYAGNIPNVTAGFNGSISRIFFFFFFFFFFFFF 360
+QLGDGR FNGVTINTFDLNGTQYPLVYAGNIPN+T GFNGSIS
Sbjct: 335 GIQLGDGRSFNGVTINTFDLNGTQYPLVYAGNIPNITGGFNGSIS--------------- 394
Query: 361 FWEKRLCSGNTVDRELVKGKIAVCDSILSPTNLGSLVSAVGIIMQDKNPKDRASSFPLPA 420
R C N+VDRE VKGKI +CD +SP NLGSL A+GIIMQD NPKD FPLPA
Sbjct: 395 ----RFCLPNSVDRESVKGKIVLCDFFVSPKNLGSLEGAIGIIMQDTNPKDLTFPFPLPA 454
Query: 421 SHLGTQQGALISSYLNLSRLPTATILKSTEGKHETAPFVASFSSRGPNPITPHILKPDLS 480
SHLGTQ+GALISSY NL+ LPTATILKSTEGK++ PFVASFSSRGPNPITP ILKPDLS
Sbjct: 455 SHLGTQEGALISSYANLTGLPTATILKSTEGKYKATPFVASFSSRGPNPITPDILKPDLS 514
Query: 481 GPGVEILAAWSPLASPSRAKDDKRELLFNIISGTSMSCPHATAVAAYVKSFHPSWSPAAL 540
GPGVEILAAWSP+ PS A++D R+LLFNIISGTSMSCPHATAVAAYVK+FHPSWSPAAL
Sbjct: 515 GPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYVKTFHPSWSPAAL 574
Query: 541 KSALMTTAYLMRAELNPDAEFAYGSGYINPLGTVNPRLIYNASEIDYIRFLCGEGYNTTL 600
KSALMTTA+ MRA+LNPDAEFAYGSG+INPL VNP LIYNA+EIDY+RFLCG+GY+T L
Sbjct: 575 KSALMTTAFPMRADLNPDAEFAYGSGHINPLSAVNPGLIYNATEIDYVRFLCGQGYSTKL 634
Query: 601 LQQISGDNSTCSPTNSDEVFDLNYPSFALFTYISTPFSQIYKRRVTNVGSTNSTYKATIF 660
+QQ+SGDNS+CS + D VFDLNYPSFAL T IST SQ+Y+RRVTNVGS NSTYKA +
Sbjct: 635 VQQVSGDNSSCSRGDIDLVFDLNYPSFALSTSISTSISQVYRRRVTNVGSANSTYKAIVS 694
Query: 661 APLGLNITVNPSILSFKALGEELKFELTIEGKISKSISSASLVWDDGVHKVRSPIIVFDS 720
P GLNITVNPS+LSFKALGEEL FELTIEG IS SI+SASLVWDDG HKV+SPI+VFD
Sbjct: 695 GPSGLNITVNPSVLSFKALGEELSFELTIEGSISSSIASASLVWDDGKHKVKSPIVVFDE 739
Query: 721 DKFTN 726
+ F N
Sbjct: 755 NTFIN 739
BLAST of HG10008957 vs. ExPASy TrEMBL
Match:
A0A0A0KJN5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G141040 PE=3 SV=1)
HSP 1 Score: 1149.0 bits (2971), Expect = 0.0e+00
Identity = 580/728 (79.67%), Postives = 630/728 (86.54%), Query Frame = 0
Query: 1 MGSHPKDQVS----TPSHHMRMLQEAIGSTFPPHSLLHSYKRSFNGFVAKLTKVEAQKVS 60
MGSH QVS + HHMR+LQEA+GSTF PH LLHSYKRSFNGFVAKLT++EA+KVS
Sbjct: 36 MGSH--HQVSSAPLSSHHHMRILQEAVGSTFAPHCLLHSYKRSFNGFVAKLTEIEAKKVS 95
Query: 61 EMKGVISIFPNGKVQLHTTRSWDFMGLSQQVNRVPSVESDIIVGVIDTGIWPESPSFLDR 120
EM+GVIS+FPNG++QLHTTRSWDFMG+S+QV RVPSVESDIIVGV DTGIWPESPSFLD
Sbjct: 96 EMEGVISVFPNGELQLHTTRSWDFMGMSEQVERVPSVESDIIVGVFDTGIWPESPSFLDH 155
Query: 121 GYGPPPPKWKGSCEVSPDFSCNNKIIGARSYRSDGRYPINDIKGPRDSNGHGTHTASTVA 180
GYGPPPPKWKGSCEVS +FSCNNKIIGARSYRSDGRYPI+DIKGPRDSNGHGTH ASTVA
Sbjct: 156 GYGPPPPKWKGSCEVSANFSCNNKIIGARSYRSDGRYPIDDIKGPRDSNGHGTHAASTVA 215
Query: 181 GGLVGGASMLGLGSGTARGGVPSARIAAYKVCWSDDCFDADILAAFDDAIADGVDIISIS 240
GGLV ASMLGLG GTARGGVPSARIAAYKVCWSD C DAD+LAAFDDAIADGVDIIS+S
Sbjct: 216 GGLVRQASMLGLGMGTARGGVPSARIAAYKVCWSDTCSDADVLAAFDDAIADGVDIISMS 275
Query: 241 VGSKTPRDNYFNDPIAIGAFHAMKKGILTSTSAGNLGPTSFTVTNFSPWSLSMAASTTDR 300
VG K PR NYF DPIAIG FHAM+ GILTSTSAGN GP FTVTNFSPW+LS+AAST+DR
Sbjct: 276 VGPKRPRPNYFQDPIAIGTFHAMRNGILTSTSAGNEGPLHFTVTNFSPWALSVAASTSDR 335
Query: 301 KFLTAVQLGDGRKFNGVTINTFDLNGTQYPLVYAGNIPNVTAGFNGSISRIFFFFFFFFF 360
+FLTAVQLGDGRKFNGVTINTFDLNGTQYPLVYAGNIPNVT GFNGS S
Sbjct: 336 RFLTAVQLGDGRKFNGVTINTFDLNGTQYPLVYAGNIPNVTGGFNGSFS----------- 395
Query: 361 FFFFFWEKRLCSGNTVDRELVKGKIAVCDSILSPTNLGSLVSAVGIIMQDKNPKDRASSF 420
R C ++VDRELVKGKIA+CDS +SP+++GSL SAVGIIMQD++PKD +F
Sbjct: 396 --------RFCLRDSVDRELVKGKIAICDSFVSPSDVGSLESAVGIIMQDRSPKDLTFAF 455
Query: 421 PLPASHLGTQQGALISSYLNLSRLPTATILKSTEGKHETAPFVASFSSRGPNPITPHILK 480
PLPASHLG QQ LISSYLN +R+PTATILKST K + AP VASFSSRGPNP +P+ILK
Sbjct: 456 PLPASHLGIQQRPLISSYLNSTRIPTATILKSTGLKLQVAPLVASFSSRGPNPTSPYILK 515
Query: 481 PDLSGPGVEILAAWSPLASPSRAKDDKRELLFNIISGTSMSCPHATAVAAYVKSFHPSWS 540
PD+ GPGVEILAAWSPL SPS AK D R+LLFNIISGTSM+CPHATAVAAYVKSFHPSWS
Sbjct: 516 PDVIGPGVEILAAWSPLRSPSNAKGDNRKLLFNIISGTSMACPHATAVAAYVKSFHPSWS 575
Query: 541 PAALKSALMTTAYLMRAELNPDAEFAYGSGYINPLGTVNPRLIYNASEIDYIRFLCGEGY 600
PAALKSAL+TTA+ MR +L P+AEFAYGSG+INPLG VNP LIYNASE DYIRFLC EGY
Sbjct: 576 PAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLIYNASETDYIRFLCDEGY 635
Query: 601 NTTLLQQISGDNSTCSPTNSDEVFDLNYPSFALFTYISTPFSQIYKRRVTNVGSTNSTYK 660
NTT L+ I+ DNSTCS T S V+DLNYPSFALFT+ISTPFSQ KRRVTNVGSTNSTYK
Sbjct: 636 NTTFLRIITKDNSTCSTTQSIRVYDLNYPSFALFTHISTPFSQTSKRRVTNVGSTNSTYK 695
Query: 661 ATIFAPLGLNITVNPSILSFKALGEELKFELTIEGKISKSISSASLVWDDGVHKVRSPII 720
ATI AP GLNITVNPSILSFKAL EEL FE+T EGKI +SI SASLVWDDGVHKVRSPII
Sbjct: 696 ATISAPSGLNITVNPSILSFKALEEELNFEVTFEGKIDRSIESASLVWDDGVHKVRSPII 742
Query: 721 VFDSDKFT 725
VFDSD FT
Sbjct: 756 VFDSDTFT 742
BLAST of HG10008957 vs. ExPASy TrEMBL
Match:
A0A6J1E2C5 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430194 PE=3 SV=1)
HSP 1 Score: 1147.9 bits (2968), Expect = 0.0e+00
Identity = 575/725 (79.31%), Postives = 626/725 (86.34%), Query Frame = 0
Query: 1 MGSHPKDQVSTPSHHMRMLQEAIGSTFPPHSLLHSYKRSFNGFVAKLTKVEAQKVSEMKG 60
MGSHPKD+V T SHH+RMLQE IGS F PHSLLHSY+RSFNGFVAKLT+VE QKVSEMKG
Sbjct: 35 MGSHPKDRVLTRSHHVRMLQETIGSHFAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKG 94
Query: 61 VISIFPNGKVQLHTTRSWDFMGLSQQVNRVPSVESDIIVGVIDTGIWPESPSFLDRGYGP 120
VIS+FPNGK QLHTTRSWDFMGLSQQ +RVPSVESDIIVGV+DTGIWPESPSFLD+GYGP
Sbjct: 95 VISVFPNGKKQLHTTRSWDFMGLSQQASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGP 154
Query: 121 PPPKWKGSCEVSPDFSCNNKIIGARSYRSDGRYPINDIKGPRDSNGHGTHTASTVAGGLV 180
PP KWKGSCEVS DFSCNNKIIGARSYR++G+YPINDI+GPRDSNGHGTH ASTVAGGLV
Sbjct: 155 PPRKWKGSCEVSSDFSCNNKIIGARSYRTNGQYPINDIQGPRDSNGHGTHAASTVAGGLV 214
Query: 181 GGASMLGLGSGTARGGVPSARIAAYKVCWSDDCFDADILAAFDDAIADGVDIISISVGSK 240
ASMLGLGSGTARGGVPSARIA+YK+CWSD C DADILAAFDDAIADGVDIIS SVG +
Sbjct: 215 RQASMLGLGSGTARGGVPSARIASYKICWSDGCPDADILAAFDDAIADGVDIISFSVGGQ 274
Query: 241 TPRDNYFNDPIAIGAFHAMKKGILTSTSAGNLGPTSFTVTNFSPWSLSMAASTTDRKFLT 300
TPRD YFND IAIGAFHAMKK ILTS SAGN GP SFTV NFSPWSLS+AASTTDRKFLT
Sbjct: 275 TPRD-YFNDSIAIGAFHAMKKEILTSMSAGNNGPKSFTVRNFSPWSLSVAASTTDRKFLT 334
Query: 301 AVQLGDGRKFNGVTINTFDLNGTQYPLVYAGNIPNVTAGFNGSISRIFFFFFFFFFFFFF 360
VQLGDGR FNGVTINTFDLNGTQYPLVYAGNIPN+ GFNGSIS
Sbjct: 335 GVQLGDGRSFNGVTINTFDLNGTQYPLVYAGNIPNI--GFNGSIS--------------- 394
Query: 361 FWEKRLCSGNTVDRELVKGKIAVCDSILSPTNLGSLVSAVGIIMQDKNPKDRASSFPLPA 420
R C N+VD+E VKGKI +CD + PTNL L A+GIIMQD NPKD FPLPA
Sbjct: 395 ----RFCLTNSVDKESVKGKIVLCDFFVLPTNLNFLEGAIGIIMQDNNPKDLTFPFPLPA 454
Query: 421 SHLGTQQGALISSYLNLSRLPTATILKSTEGKHETAPFVASFSSRGPNPITPHILKPDLS 480
SHLGTQ+GALISSY NL+ LPTATILKSTEGK++ PFVASFSSRGPNPITP ILKPDLS
Sbjct: 455 SHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRGPNPITPDILKPDLS 514
Query: 481 GPGVEILAAWSPLASPSRAKDDKRELLFNIISGTSMSCPHATAVAAYVKSFHPSWSPAAL 540
GPGVEILAAWSP+ PS A++D R+LLFNIISGTSMSCPHATAVAAYVK+FHPSWSPAAL
Sbjct: 515 GPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYVKTFHPSWSPAAL 574
Query: 541 KSALMTTAYLMRAELNPDAEFAYGSGYINPLGTVNPRLIYNASEIDYIRFLCGEGYNTTL 600
KSALMTTA+ MR++LNPDAEFAYGSG+INPLG VNP LIYNA+EIDY+RFLCG+GY+T L
Sbjct: 575 KSALMTTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNATEIDYVRFLCGQGYSTKL 634
Query: 601 LQQISGDNSTCSPTNSDEVFDLNYPSFALFTYISTPFSQIYKRRVTNVGSTNSTYKATIF 660
+QQ+SGDNS+CS ++S+ VFDLNYPSFAL T ISTP SQ+Y+RRVTNVGS NSTY A +
Sbjct: 635 IQQVSGDNSSCSRSDSNVVFDLNYPSFALSTSISTPISQVYRRRVTNVGSANSTYNAIVS 694
Query: 661 APLGLNITVNPSILSFKALGEELKFELTIEGKISKSISSASLVWDDGVHKVRSPIIVFDS 720
P L ITVNPS+LSFKALGEEL FE+TIEG IS I SASLVWDDG HKVRSP+IVFDS
Sbjct: 695 GPSRLKITVNPSVLSFKALGEELSFEVTIEGSISSGIVSASLVWDDGQHKVRSPVIVFDS 737
Query: 721 DKFTN 726
F N
Sbjct: 755 TSFIN 737
BLAST of HG10008957 vs. TAIR 10
Match:
AT5G59190.1 (subtilase family protein )
HSP 1 Score: 668.3 bits (1723), Expect = 6.9e-192
Identity = 365/724 (50.41%), Postives = 470/724 (64.92%), Query Frame = 0
Query: 1 MGSHPKDQVSTPSHHMRMLQEAIGSTFPPHSLLHSYKRSFNGFVAKLTKVEAQKVSEMKG 60
MG+ P+ + S PSHH+ +LQ+ +G+ H L+ SYKRSFNGF A L++ E+QK+ MK
Sbjct: 1 MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60
Query: 61 VISIFPNGKVQLHTTRSWDFMGLSQQVNRVPSVESDIIVGVIDTGIWPESPSFLDRGYGP 120
V+S+FP+ +L TTRSWDF+G ++ R ESD+IVGVID+GIWPES SF D G+GP
Sbjct: 61 VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 120
Query: 121 PPPKWKGSCEVSPDFSCNNKIIGARSYRSDGRYPINDIKGPRDSNGHGTHTASTVAGGLV 180
PP KWKGSC+ F+CNNK+IGAR Y RD GHGTHTAST AG V
Sbjct: 121 PPKKWKGSCKGGLKFACNNKLIGARFYNKFA-------DSARDEEGHGTHTASTAAGNAV 180
Query: 181 GGASMLGLGSGTARGGVPSARIAAYKVCWSDDCFDADILAAFDDAIADGVDIISISVGSK 240
AS GL GTARGGVPSARIAAYKVC+ + C D DILAAFDDAIADGVD+ISIS+ S
Sbjct: 181 QAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISI-SA 240
Query: 241 TPRDNYFNDPIAIGAFHAMKKGILTSTSAGNLGPTSFTVTNFSPWSLSMAASTTDRKFLT 300
N N +AIG+FHAM +GI+T+ SAGN GP +V N SPW +++AAS TDR+F+
Sbjct: 241 DYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFID 300
Query: 301 AVQLGDGRKFNGVTINTFDLNGTQYPLVYAGNIPNVTAGFNGSISRIFFFFFFFFFFFFF 360
V LG+G+ G+++NTF+LNGT++P+VY N+ N S ++ +
Sbjct: 301 RVVLGNGKALTGISVNTFNLNGTKFPIVYGQNVSR-----NCSQAQAGY----------- 360
Query: 361 FWEKRLCSGNTVDRELVKGKIAVCDSILSPTNLGSLVSAVGIIMQDKNPKDRASSFPLPA 420
CS VD ELVKGKI +CD L L A+G+I+Q+ D A P PA
Sbjct: 361 ------CSSGCVDSELVKGKIVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPA 420
Query: 421 SHLGTQQGALISSYLNLSRLPTATILKSTEGKHETAPFVASFSSRGPNPITPHILKPDLS 480
S LG + I SY+ + P A IL++ E AP+V SFSSRGP+ + ++LKPD+S
Sbjct: 421 SSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVS 480
Query: 481 GPGVEILAAWSPLASPSR--AKDDKRELLFNIISGTSMSCPHATAVAAYVKSFHPSWSPA 540
PG+EILAA+SP+ASPS +DKR + ++++SGTSM+CPH VAAYVKSFHP WSP+
Sbjct: 481 APGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPS 540
Query: 541 ALKSALMTTAYLMRAELNPDAEFAYGSGYINPLGTVNPRLIYNASEIDYIRFLCGEGYNT 600
A+KSA+MTTA M + NP+ EFAYGSG INP +P L+Y DY++ LC EG+++
Sbjct: 541 AIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDS 600
Query: 601 TLLQQISGDNSTCSPTNSDEVFDLNYPSFALFTYISTPFSQIYKRRVTNVGSTNSTYKAT 660
T L SG N TCS EV DLNYP+ F PF+ +KR VTNVG NSTYKA+
Sbjct: 601 TTLTTTSGQNVTCS--ERTEVKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKAS 660
Query: 661 IFAPL--GLNITVNPSILSFKALGEELKFELTIEGKISK--SISSASLVWDDGVHKVRSP 719
+ PL L I++ P IL F L E+ F +TI GK K S S+S+VW DG H VRSP
Sbjct: 661 V-VPLQPELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSP 689
BLAST of HG10008957 vs. TAIR 10
Match:
AT5G59120.1 (subtilase 4.13 )
HSP 1 Score: 628.2 bits (1619), Expect = 7.9e-180
Identity = 348/731 (47.61%), Postives = 464/731 (63.47%), Query Frame = 0
Query: 1 MGSHPKDQVSTP-SHHMRMLQEAIGSTFPPHSLLHSYKRSFNGFVAKLTKVEAQKVSEMK 60
MGS TP S HM +LQE G + L+ SYKRSFNGF A+LT+ E ++V++M
Sbjct: 36 MGSLSSRADYTPTSDHMNILQEVTGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMV 95
Query: 61 GVISIFPNGKVQLHTTRSWDFMGLSQ--QVNRVPSVESDIIVGVIDTGIWPESPSFLDRG 120
GV+S+FPN K+QL TT SWDFMGL + + R P+VESD I+GVID+GI PES SF D+G
Sbjct: 96 GVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKG 155
Query: 121 YGPPPPKWKGSCEVSPDFSCNNKIIGARSYRSDGRYPINDIKGPRDSNGHGTHTASTVAG 180
+GPPP KWKG C +F+CNNK+IGAR Y S+ G RD +GHGTHTAST AG
Sbjct: 156 FGPPPQKWKGVCSGGKNFTCNNKLIGARDYTSE---------GTRDMDGHGTHTASTAAG 215
Query: 181 GLVGGASMLGLGSGTARGGVPSARIAAYKVCWSDDCFDADILAAFDDAIADGVDIISISV 240
V AS G+G+GT RGGVP++R+AAYKVC C +L+AFDDAIADGVD+I+IS+
Sbjct: 216 NAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISI 275
Query: 241 GSKTPRDNYFNDPIAIGAFHAMKKGILTSTSAGNLGPTSFTVTNFSPWSLSMAASTTDRK 300
G KT + NDPIAIGAFHAM KG+LT SAGN GP +V+ +PW L++AASTT+R
Sbjct: 276 GDKT-ASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRG 335
Query: 301 FLTAVQLGDGRKFNGVTINTFDLNGTQYPLVYAGNIPNVTAGFNGSISRIFFFFFFFFFF 360
F+T V LG+G+ G ++N +++ G YPLVY + + +
Sbjct: 336 FVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSAASSACDAESA-------------- 395
Query: 361 FFFFWEKRLCSGNTVDRELVKGKIAVCDSILSPTNLGSLVSAVGIIMQDKNPKDRASSFP 420
LC + VD+ VKGKI VC + V AVG+I + P D A P
Sbjct: 396 -------GLCELSCVDKSRVKGKILVCGG-PGGLKIVESVGAVGLIYRTPKP-DVAFIHP 455
Query: 421 LPASHLGTQQGALISSYLNLSRLPTATILKSTEGKHETAPFVASFSSRGPNPITPHILKP 480
LPA+ L T+ + SYL + P A +LK+ + T+P +ASFSSRGPN I ILKP
Sbjct: 456 LPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKP 515
Query: 481 DLSGPGVEILAAWSPLASPSRAKDDKRELLFNIISGTSMSCPHATAVAAYVKSFHPSWSP 540
D++ PGVEILAA+SP PS +DD R + ++++SGTSMSCPH VAAYVK+F+P WSP
Sbjct: 516 DITAPGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSP 575
Query: 541 AALKSALMTTAYLMRAELN--PDAEFAYGSGYINPLGTVNPRLIYNASEIDYIRFLCGEG 600
+ ++SA+MTTA+ + A EFAYGSG+++P+ NP L+Y + D+I FLCG
Sbjct: 576 SMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMN 635
Query: 601 YNTTLLQQISGDNSTCSPTNSDEVFDLNYPSF-ALFTYISTPFSQIYKRRVTNVGSTNST 660
Y + +L+ ISG+ TCS +LNYPS A + T F+ + R +TNVG+ NST
Sbjct: 636 YTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNST 695
Query: 661 YKATIFAPLG--LNITVNPSILSFKALGEELKFELTIEGK--ISKSISSASLVWDDGVHK 720
Y + + A G L++ + PS+LSFK + E+ F +T+ G S+ SSA+L+W DG H
Sbjct: 696 YTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSDGTHN 731
Query: 721 VRSPIIVFDSD 722
VRSPI+V+ SD
Sbjct: 756 VRSPIVVYTSD 731
BLAST of HG10008957 vs. TAIR 10
Match:
AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 622.5 bits (1604), Expect = 4.4e-178
Identity = 343/734 (46.73%), Postives = 475/734 (64.71%), Query Frame = 0
Query: 1 MGSHPKDQVSTP-SHHMRMLQEAIGSTFPPHSLLHSYKRSFNGFVAKLTKVEAQKVSEMK 60
+GS P + TP S HM +LQE G + + L+ SYK+SFNGF A+LT+ E ++++ M+
Sbjct: 39 LGSLPSREEYTPMSDHMSILQEITGESLIENRLVRSYKKSFNGFAARLTESERKRLAGME 98
Query: 61 GVISIFPNGKVQLHTTRSWDFMGLSQ--QVNRVPSVESDIIVGVIDTGIWPESPSFLDRG 120
V+S+FP+ K++L TT SW+FMGL + + R S+ESD I+GVID+GI+PES SF D+G
Sbjct: 99 RVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQG 158
Query: 121 YGPPPPKWKGSCEVSPDFSCNNKIIGARSYRSDGRYPINDIKGPRDSNGHGTHTASTVAG 180
+GPPP KWKG+C +F+CNNK+IGAR Y + + + RD +GHGTHTAS AG
Sbjct: 159 FGPPPKKWKGTCAGGKNFTCNNKVIGARDYTAKSKAN----QTARDYSGHGTHTASIAAG 218
Query: 181 GLVGGASMLGLGSGTARGGVPSARIAAYKVCWSDDCFDADILAAFDDAIADGVDIISISV 240
V ++ GLG+GTARGGVP+ARIA YKVC ++ C +++AFDDAIADGVD+ISIS+
Sbjct: 219 NAVANSNFYGLGNGTARGGVPAARIAVYKVCDNEGCDGEAMMSAFDDAIADGVDVISISI 278
Query: 241 GSKTPRDN---YFNDPIAIGAFHAMKKGILTSTSAGNLGPTSFTVTNFSPWSLSMAASTT 300
DN + DPIAIGAFHAM G+LT +AGN GP TVT+ +PW S+AAS T
Sbjct: 279 ----VLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGPKISTVTSTAPWVFSVAASVT 338
Query: 301 DRKFLTAVQLGDGRKFNGVTINTFDLNGTQYPLVYAGNIPNVTAGFNGSISRIFFFFFFF 360
+R F+ V LGDG+ G ++NT+D+NGT YPLVY + T +
Sbjct: 339 NRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSAALSTCSVD------------- 398
Query: 361 FFFFFFFWEKRLCSGNTVDRELVKGKIAVCDS---ILSPTNLGSLVSAVGIIMQDKNPKD 420
+ RLC +D +LVKGKI +CDS ++ LG AVG I+++ P D
Sbjct: 399 --------KARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLG----AVGSIVKNPEP-D 458
Query: 421 RASSFPLPASHLGTQQGALISSYLNLSRLPTATILKSTEGKHETAPFVASFSSRGPNPIT 480
RA P S L + SY+N ++ P AT+LKS E ++ AP VASFSSRGP+ I
Sbjct: 459 RAFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIV 518
Query: 481 PHILKPDLSGPGVEILAAWSPLASPSRAKDDKRELLFNIISGTSMSCPHATAVAAYVKSF 540
ILKPD++ PGVEILAA+SP +SP+ ++ D R + ++++SGTSM+CPH VAAYVK+F
Sbjct: 519 SDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTF 578
Query: 541 HPSWSPAALKSALMTTAYLMRAELN--PDAEFAYGSGYINPLGTVNPRLIYNASEIDYIR 600
HP WSP+ ++SA+MTTA+ M A + EFAYGSG+++P+ +NP L+Y ++ D+I
Sbjct: 579 HPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHIN 638
Query: 601 FLCGEGYNTTLLQQISGDNSTCSPTNSDEV-FDLNYPSFALFTYISTPFSQIYKRRVTNV 660
FLCG Y + L+ ISGDNSTC+ S + +LNYP+ + + PF+ ++R VTNV
Sbjct: 639 FLCGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQRTVTNV 698
Query: 661 GSTNSTYKATI--FAPLGLNITVNPSILSFKALGEELKFELTIEGKI--SKSISSASLVW 719
G STY A + F L+I V+P +LS K++ E+ F +T+ +K SA+L+W
Sbjct: 699 GMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQPVSANLIW 738
BLAST of HG10008957 vs. TAIR 10
Match:
AT5G59090.1 (subtilase 4.12 )
HSP 1 Score: 615.9 bits (1587), Expect = 4.1e-176
Identity = 338/716 (47.21%), Postives = 460/716 (64.25%), Query Frame = 0
Query: 13 SHHMRMLQEAIGSTFPPHSLLHSYKRSFNGFVAKLTKVEAQKVSEMKGVISIFPNGKVQL 72
S HM +LQ+ G + L+ SYKRSFNGF A+LT+ E ++E++GV+S+FPN +QL
Sbjct: 50 SDHMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQL 109
Query: 73 HTTRSWDFMGLSQQVN--RVPSVESDIIVGVIDTGIWPESPSFLDRGYGPPPPKWKGSCE 132
HTT SWDFMG+ + N R ++ESD I+GVIDTGIWPES SF D+G+GPPP KWKG C
Sbjct: 110 HTTTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCS 169
Query: 133 VSPDFSCNNKIIGARSYRSDGRYPINDIKGPRDSNGHGTHTASTVAGGLVGGASMLGLGS 192
+F+CNNK+IGAR Y S+ G RD++GHGTHTAST AG V S G+G+
Sbjct: 170 GGKNFTCNNKLIGARDYTSE---------GTRDTSGHGTHTASTAAGNAVKDTSFFGIGN 229
Query: 193 GTARGGVPSARIAAYKVCWSDDCFDADILAAFDDAIADGVDIISISVGSKTPRDNYFNDP 252
GT RGGVP++RIAAYKVC C +L++FDDAIADGVD+I+IS+G + P + +DP
Sbjct: 230 GTVRGGVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFP-SIFEDDP 289
Query: 253 IAIGAFHAMKKGILTSTSAGNLGPTSFTVTNFSPWSLSMAASTTDRKFLTAVQLGDGRKF 312
IAIGAFHAM KGILT +SAGN GP TV++ +PW ++AASTT+R F+T V LG+G+
Sbjct: 290 IAIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTL 349
Query: 313 NGVTINTFDLNGTQYPLVYAGNIPNVTAGFNGSISRIFFFFFFFFFFFFFFWEKRLCSGN 372
G ++N FD+ G +YPLVY + + + LC+
Sbjct: 350 AGRSVNAFDMKGKKYPLVYGKSAASSACDAKTA---------------------ALCAPA 409
Query: 373 TVDRELVKGKIAVCDSILSPTNLGSLVSAVGIIMQDKNPK-DRASSFPLPASHLGTQQGA 432
+++ VKGKI VC S + V A+ II DK+P+ D A + LPAS L +
Sbjct: 410 CLNKSRVKGKILVCGG-PSGYKIAKSVGAIAII--DKSPRPDVAFTHHLPASGLKAKDFK 469
Query: 433 LISSYLNLSRLPTATILKSTEGKHETAPFVASFSSRGPNPITPHILKPDLSGPGVEILAA 492
+ SY+ P A +LK+ + T+P +ASFSSRGPN I ILKPD++ PGVEILAA
Sbjct: 470 SLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAA 529
Query: 493 WSPLASPSRAKDDKRELLFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALMTTAY 552
+SP PS +DD R + +++ SGTSM+CPH VAAYVK+F+P WSP+ ++SA+MTTA+
Sbjct: 530 FSPNGEPS--EDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAW 589
Query: 553 LMRAELN--PDAEFAYGSGYINPLGTVNPRLIYNASEIDYIRFLCGEGYNTTLLQQISGD 612
++A+ EFAYG+G+++P+ +NP L+Y + D+I FLCG Y + L+ ISGD
Sbjct: 590 PVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGD 649
Query: 613 NSTCSPTNSDEVFDLNYPSF-ALFTYISTPFSQIYKRRVTNVGSTNSTYKATIFAPLG-- 672
CS N +LNYPS A + + FS + R +TNVG+ NSTYK+ + A G
Sbjct: 650 TVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSK 709
Query: 673 LNITVNPSILSFKALGEELKFELTIEGK--ISKSISSASLVWDDGVHKVRSPIIVF 719
L+I V PS+L FK + E+ F +T+ G S+ SSA+L+W DG H VRSPI+V+
Sbjct: 710 LSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIVVY 729
BLAST of HG10008957 vs. TAIR 10
Match:
AT5G59090.2 (subtilase 4.12 )
HSP 1 Score: 612.5 bits (1578), Expect = 4.5e-175
Identity = 337/714 (47.20%), Postives = 456/714 (63.87%), Query Frame = 0
Query: 13 SHHMRMLQEAIGSTFPPHSLLHSYKRSFNGFVAKLTKVEAQKVSEMKGVISIFPNGKVQL 72
S HM +LQ+ G + L+ SYKRSFNGF A+LT+ E ++E++GV+S+FPN +QL
Sbjct: 50 SDHMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQL 109
Query: 73 HTTRSWDFMGLSQQVN--RVPSVESDIIVGVIDTGIWPESPSFLDRGYGPPPPKWKGSCE 132
HTT SWDFMG+ + N R ++ESD I+GVIDTGIWPES SF D+G+GPPP KWKG C
Sbjct: 110 HTTTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCS 169
Query: 133 VSPDFSCNNKIIGARSYRSDGRYPINDIKGPRDSNGHGTHTASTVAGGLVGGASMLGLGS 192
+F+CNNK+IGAR Y S+ G RD++GHGTHTAST AG V S G+G+
Sbjct: 170 GGKNFTCNNKLIGARDYTSE---------GTRDTSGHGTHTASTAAGNAVKDTSFFGIGN 229
Query: 193 GTARGGVPSARIAAYKVCWSDDCFDADILAAFDDAIADGVDIISISVGSKTPRDNYFNDP 252
GT RGGVP++RIAAYKVC C +L++FDDAIADGVD+I+IS+G + P + +DP
Sbjct: 230 GTVRGGVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFP-SIFEDDP 289
Query: 253 IAIGAFHAMKKGILTSTSAGNLGPTSFTVTNFSPWSLSMAASTTDRKFLTAVQLGDGRKF 312
IAIGAFHAM KGILT +SAGN GP TV++ +PW ++AASTT+R F+T V LG+G+
Sbjct: 290 IAIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTL 349
Query: 313 NGVTINTFDLNGTQYPLVYAGNIPNVTAGFNGSISRIFFFFFFFFFFFFFFWEKRLCSGN 372
G ++N FD+ G +YPLVY + + + LC+
Sbjct: 350 AGRSVNAFDMKGKKYPLVYGKSAASSACDAKTA---------------------ALCAPA 409
Query: 373 TVDRELVKGKIAVCDSILSPTNLGSLVSAVGIIMQDKNPK-DRASSFPLPASHLGTQQGA 432
+++ VKGKI VC S + V A+ II DK+P+ D A + LPAS L +
Sbjct: 410 CLNKSRVKGKILVCGG-PSGYKIAKSVGAIAII--DKSPRPDVAFTHHLPASGLKAKDFK 469
Query: 433 LISSYLNLSRLPTATILKSTEGKHETAPFVASFSSRGPNPITPHILKPDLSGPGVEILAA 492
+ SY+ P A +LK+ + T+P +ASFSSRGPN I ILKPD++ PGVEILAA
Sbjct: 470 SLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAA 529
Query: 493 WSPLASPSRAKDDKRELLFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALMTTAY 552
+SP PS +DD R + +++ SGTSM+CPH VAAYVK+F+P WSP+ ++SA+MTTA
Sbjct: 530 FSPNGEPS--EDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTA- 589
Query: 553 LMRAELNPDAEFAYGSGYINPLGTVNPRLIYNASEIDYIRFLCGEGYNTTLLQQISGDNS 612
+ EFAYG+G+++P+ +NP L+Y + D+I FLCG Y + L+ ISGD
Sbjct: 590 --KGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTV 649
Query: 613 TCSPTNSDEVFDLNYPSF-ALFTYISTPFSQIYKRRVTNVGSTNSTYKATIFAPLG--LN 672
CS N +LNYPS A + + FS + R +TNVG+ NSTYK+ + A G L+
Sbjct: 650 KCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLS 709
Query: 673 ITVNPSILSFKALGEELKFELTIEGK--ISKSISSASLVWDDGVHKVRSPIIVF 719
I V PS+L FK + E+ F +T+ G S+ SSA+L+W DG H VRSPI+V+
Sbjct: 710 IKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIVVY 724
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q39547 | 4.8e-222 | 55.99 | Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1 | [more] |
| Q9FIF8 | 9.8e-191 | 50.41 | Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... | [more] |
| Q9FIG2 | 1.1e-178 | 47.61 | Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2... | [more] |
| Q9FGU3 | 6.1e-177 | 46.73 | Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 S... | [more] |
| Q8L7D2 | 5.7e-175 | 47.21 | Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A1S3AUC4 | 0.0e+00 | 81.71 | cucumisin-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482910 PE=3 SV=1 | [more] |
| A0A1S3AUU4 | 0.0e+00 | 81.66 | cucumisin-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103482910 PE=3 SV=1 | [more] |
| A0A6J1E2G4 | 0.0e+00 | 78.62 | cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430231 PE=3 SV=1 | [more] |
| A0A0A0KJN5 | 0.0e+00 | 79.67 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G141040 PE=3 SV=1 | [more] |
| A0A6J1E2C5 | 0.0e+00 | 79.31 | cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430194 PE=3 SV=1 | [more] |
