HG10008896 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10008896 vs. NCBI nr
Match: XP_038874841.1 (probable pectinesterase/pectinesterase inhibitor 61 [Benincasa hispida])

HSP 1 Score: 565.1 bits (1455), Expect = 4.0e-157
Identity = 296/326 (90.80%), Postives = 307/326 (94.17%), Query Frame = 0

Query: 1   MINNNYNKLHSSRSVDSTPATSFHLSSND---PPPPSSSHLPRSRVLIISFLSVFLIAAS 60
           MINNNYNKLHSS S  STPA+SFHLS ND    PPPSSSH  RSR+LIISFLSV LIAAS
Sbjct: 1   MINNNYNKLHSSLSATSTPASSFHLSGNDLPPTPPPSSSHRRRSRILIISFLSVLLIAAS 60

Query: 61  VVSAAVLVRSRIASNQPHFPHNNPSQAISRACSHTLYPSLCLSSLLSFPGSLSADVHDLV 120
           +VSAAVLVRSRIASN+PHFPHN PSQAISRACSHTLYPSLCLSSLLS P +LSADVHDLV
Sbjct: 61  IVSAAVLVRSRIASNEPHFPHNKPSQAISRACSHTLYPSLCLSSLLSIPDALSADVHDLV 120

Query: 121 HISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSLSLFSPEAS 180
           HISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFS+SLFS EAS
Sbjct: 121 HISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSVSLFSTEAS 180

Query: 181 NHDVMTWLSAALTYHDTCTDGFADVADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDL 240
           NHDVMTWLSAALTYHDTCTDGFADVADLAVK+QVEAKLSDLSEMISNSLAIFSGFGGGDL
Sbjct: 181 NHDVMTWLSAALTYHDTCTDGFADVADLAVKNQVEAKLSDLSEMISNSLAIFSGFGGGDL 240

Query: 241 PVQNRRRRLMESGAQPWTAENGGDGGFPQWLSGKDRRLLASPISSIQADIVVAKDGSGKF 300
           PVQNRRRRLMESG + W AENGGDGGFPQWLS KDRRLLA+PIS+IQADIVVAKDGSGKF
Sbjct: 241 PVQNRRRRLMESGTRSWAAENGGDGGFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGKF 300

Query: 301 KTVAEAIEAAPSSSGRRIVIYIKAGK 324
           KTVAEAI+AAPSSSGRRIVIYIKAGK
Sbjct: 301 KTVAEAIDAAPSSSGRRIVIYIKAGK 326

BLAST of HG10008896 vs. NCBI nr
Match: XP_008437596.1 (PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor 61 [Cucumis melo])

HSP 1 Score: 532.3 bits (1370), Expect = 2.8e-147
Identity = 280/324 (86.42%), Postives = 298/324 (91.98%), Query Frame = 0

Query: 3   NNNYNKLHSSRSVDSTPATSFHLSSNDPPPPSSSHLPRSRVLIISFLSVFLIAASVVSAA 62
           NNNY +LH+S S ++TPATSF LS NDPPPPSSS   RSR+LIISF+SV LIAASVVSAA
Sbjct: 4   NNNYKQLHTSHSANTTPATSFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAA 63

Query: 63  VLVRSRIASNQPHFPHNNPSQAISRACSHTLYPSLCLSSLLSFPGSLSADVHDLVHISLN 122
           +LVRSRIAS+QPHFPHN PSQAISRACSHTLYPSLCLSSLLSFPG+ SADVHDLVHISLN
Sbjct: 64  ILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHISLN 123

Query: 123 LTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSLSLFSPEASNHDVM 182
           LTLQHLTKALYSTSQIPI QIS DPLAHSAYEDCMELLNDA+DAFSLSLFS  ASNHD+M
Sbjct: 124 LTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNHDIM 183

Query: 183 TWLSAALTYHDTCTDGFADVADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDLPVQNR 242
           TWLSAALTYHDTCTDGF DV DL VK++VEAKLSDLSEMISNSLAIFSGFGGGDLPV+NR
Sbjct: 184 TWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENR 243

Query: 243 -RRRLMESGA-QPWTAENGGD-GGFPQWLSGKDRRLLASPISSIQADIVVAKDGSGKFKT 302
            RRRLMESG    W AENGGD  GFP+WLSGKDRRLLA+PIS+IQADIVVAKDGSGKFKT
Sbjct: 244 KRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGKFKT 303

Query: 303 VAEAIEAAPSSSGRRIVIYIKAGK 324
           VAEAIEAAPSSSGRRI+IYIKAGK
Sbjct: 304 VAEAIEAAPSSSGRRIIIYIKAGK 327

BLAST of HG10008896 vs. NCBI nr
Match: KAA0042551.1 (putative pectinesterase/pectinesterase inhibitor 61 [Cucumis melo var. makuwa] >TYK05955.1 putative pectinesterase/pectinesterase inhibitor 61 [Cucumis melo var. makuwa])

HSP 1 Score: 532.3 bits (1370), Expect = 2.8e-147
Identity = 280/324 (86.42%), Postives = 298/324 (91.98%), Query Frame = 0

Query: 3   NNNYNKLHSSRSVDSTPATSFHLSSNDPPPPSSSHLPRSRVLIISFLSVFLIAASVVSAA 62
           NNNY +LH+S S ++TPATSF LS NDPPPPSSS   RSR+LIISF+SV LIAASVVSAA
Sbjct: 4   NNNYKQLHTSHSANTTPATSFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAA 63

Query: 63  VLVRSRIASNQPHFPHNNPSQAISRACSHTLYPSLCLSSLLSFPGSLSADVHDLVHISLN 122
           +LVRSRIAS+QPHFPHN PSQAISRACSHTLYPSLCLSSLLSFPG+ SADVHDLVHISLN
Sbjct: 64  ILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHISLN 123

Query: 123 LTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSLSLFSPEASNHDVM 182
           LTLQHLTKALYSTSQIPI QIS DPLAHSAYEDCMELLNDA+DAFSLSLFS  ASNHD+M
Sbjct: 124 LTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNHDIM 183

Query: 183 TWLSAALTYHDTCTDGFADVADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDLPVQNR 242
           TWLSAALTYHDTCTDGF DV DL VK++VEAKLSDLSEMISNSLAIFSGFGGGDLPV+NR
Sbjct: 184 TWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENR 243

Query: 243 -RRRLMESGA-QPWTAENGGD-GGFPQWLSGKDRRLLASPISSIQADIVVAKDGSGKFKT 302
            RRRLMESG    W AENGGD  GFP+WLSGKDRRLLA+PIS+IQADIVVAKDGSGKFKT
Sbjct: 244 KRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGKFKT 303

Query: 303 VAEAIEAAPSSSGRRIVIYIKAGK 324
           VAEAIEAAPSSSGRRI+IYIKAGK
Sbjct: 304 VAEAIEAAPSSSGRRIIIYIKAGK 327

BLAST of HG10008896 vs. NCBI nr
Match: XP_011654619.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucumis sativus] >KGN49865.1 hypothetical protein Csa_000423 [Cucumis sativus])

HSP 1 Score: 529.3 bits (1362), Expect = 2.4e-146
Identity = 275/323 (85.14%), Postives = 295/323 (91.33%), Query Frame = 0

Query: 3   NNNYNKLHSSRSVDSTPATSFHLSSNDPPPPSSSHLPRSRVLIISFLSVFLIAASVVSAA 62
           NN+YNKLH+S S ++TPA SF LS NDPPPPSSS   RSR+LIISF+SV LIAASVVSAA
Sbjct: 4   NNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAA 63

Query: 63  VLVRSRIASNQPHFPHNNPSQAISRACSHTLYPSLCLSSLLSFPGSLSADVHDLVHISLN 122
           +LVRS + S+QPHFPHN PSQAISRACSHTLYPSLCLSSLLSFPG+ SADVHDLVHISLN
Sbjct: 64  ILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHISLN 123

Query: 123 LTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSLSLFSPEASNHDVM 182
           LTLQHLTKALYSTSQIP+ QIS DPLAHSAYEDCMELLNDA+DAFSLSLFS +ASNHD+M
Sbjct: 124 LTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNHDIM 183

Query: 183 TWLSAALTYHDTCTDGFADVADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDLPVQNR 242
           TWLSAALTYHDTCT GF DVADL VKD+VEAKLSDLSEMISNSLAIFSGFGGGDLPV+NR
Sbjct: 184 TWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENR 243

Query: 243 -RRRLMESGAQPWTAENGGD-GGFPQWLSGKDRRLLASPISSIQADIVVAKDGSGKFKTV 302
            RRRLMES    W AENGGD  GFP WLSGKDRRLLA+P+S+IQADIVVAKDGSGKFKTV
Sbjct: 244 KRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTV 303

Query: 303 AEAIEAAPSSSGRRIVIYIKAGK 324
           AEAIEAAPSSSGRRI+IYIKAGK
Sbjct: 304 AEAIEAAPSSSGRRIIIYIKAGK 326

BLAST of HG10008896 vs. NCBI nr
Match: KAG6579321.1 (putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 488.4 bits (1256), Expect = 4.7e-134
Identity = 266/326 (81.60%), Postives = 286/326 (87.73%), Query Frame = 0

Query: 2   INNNYNKLHSSRSVDSTPATSFHLSS--NDPPPPSSSHLPRSRVLIISFLSVFLIAASVV 61
           +NNNYNKLHSSRS D TPA+SF +S+  +DP PP S    R R+L+ISFLS+FLI AS V
Sbjct: 1   MNNNYNKLHSSRSGD-TPASSFRVSNDHSDPLPPPSPSSSRRRILLISFLSIFLITASAV 60

Query: 62  SAAVLVRSRI-ASNQPHFPHNNPSQAISRACSHTLYPSLCLSSLLSFPGSLSADVHDLVH 121
           SA VLV SRI AS + HFPHN P+QAISRACS TLYPSLCLSSLLS PGSLSADVHDLVH
Sbjct: 61  SAVVLVHSRIAASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLVH 120

Query: 122 ISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSLSLFSPEASN 181
           ISLNLTLQHLTKALYSTSQIPISQISNDPLA SAYEDCMELLNDAV+AFSLSLFS  A+N
Sbjct: 121 ISLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTATN 180

Query: 182 HDVMTWLSAALTYHDTCTDGFADVADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDLP 241
           HDVMTWLSAALTYHDTCTDGF+DVAD  +KDQ+ AKLSDLSEMISNSLAIFSGFGG DLP
Sbjct: 181 HDVMTWLSAALTYHDTCTDGFSDVAD-PIKDQITAKLSDLSEMISNSLAIFSGFGGDDLP 240

Query: 242 VQNRRRRLMESGAQPWTA-ENGGDGGFPQWLSGKDRRLLASPISSIQADIVVAKDGSGKF 301
           V+NRRRR +    + W A EN GDG FPQW+SGKDRRLLA+PISSIQADIVVAKDGSGKF
Sbjct: 241 VENRRRRRLMESDEHWLAEENNGDGDFPQWVSGKDRRLLAAPISSIQADIVVAKDGSGKF 300

Query: 302 KTVAEAIEAAPSSSGRRIVIYIKAGK 324
           KTVAEAI AAPSSSGRRIVIYIKAGK
Sbjct: 301 KTVAEAIAAAPSSSGRRIVIYIKAGK 324

BLAST of HG10008896 vs. ExPASy Swiss-Prot
Match: Q9M3B0 (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3702 GN=PME34 PE=2 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 2.3e-59
Identity = 139/321 (43.30%), Postives = 201/321 (62.62%), Query Frame = 0

Query: 12  SRSVDSTPATSFHLSSNDPPPPSSSHLPRSRVLIISF-LSVFLIAASVVSAAVLVRSRIA 71
           S +  +T A   +  S    P +++   + ++++ S  L++ LI A+ + A V  R ++ 
Sbjct: 14  SVTTSTTTAPILNQVSTSEQPENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLN 73

Query: 72  SNQPHFPHNNPSQAISRACSHTLYPSLCLSSLLSFPGSLSA-DVHDLVHISLNLTLQHLT 131
            + P      PSQAIS+AC  T +P LC+ SL+ FPGSL+A    DL+H+++N+TL H +
Sbjct: 74  QSVPGLA-RKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFS 133

Query: 132 KALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFS---LSLFSPEASNHDVMTWLS 191
            ALYS++   +S +   P A SAY+ C+ELL+D+VDA S    S+ S  A   DV TWLS
Sbjct: 134 HALYSSAS--LSFVDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLS 193

Query: 192 AALTYHDTCTDGFADVADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGD----LPVQNR 251
           AALT HDTCT+GF  V D  VKD + A L +LSE++SN LAIFS    GD    +P+QNR
Sbjct: 194 AALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNR 253

Query: 252 RRRLMESGAQPWTAENGGDGGFPQWLSGKDRRLLASPISSIQADIVVAKDGSGKFKTVAE 311
           R   +E   +           FP+W+  K+R +L  P+S IQADI+V+KDG+G  KT++E
Sbjct: 254 RLLGVEEREEK----------FPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISE 313

Query: 312 AIEAAPSSSGRRIVIYIKAGK 324
           AI+ AP +S RRI+IY+KAG+
Sbjct: 314 AIKKAPQNSTRRIIIYVKAGR 321

BLAST of HG10008896 vs. ExPASy Swiss-Prot
Match: Q9FK05 (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3702 GN=PME61 PE=2 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 1.7e-57
Identity = 136/304 (44.74%), Postives = 184/304 (60.53%), Query Frame = 0

Query: 31  PPPSSSHLPRSRVLIISFLSVFLIAASVVSAAVLVRSRIASNQPHFPH--NNPSQAISRA 90
           P  S   L +     +   ++ ++   VV   +    R   +    P     P+QAISR 
Sbjct: 19  PATSLPELQKKTKTKLILFTLAVLVVGVVCFGIFAGIRAVDSGKTEPKLTRKPTQAISRT 78

Query: 91  CSHTLYPSLCLSSLLSFPGSLSADVHDLVHISLNLTLQHLTKALYSTSQIPISQISNDPL 150
           CS +LYP+LC+ +LL FPGSL+AD ++L+HIS N TLQ  +KALY++S I  +Q+   P 
Sbjct: 79  CSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSSTITYTQM--PPR 138

Query: 151 AHSAYEDCMELLNDAVDAF-----SLSLFSPEASNHDVMTWLSAALTYHDTCTDGFADVA 210
             SAY+ C+ELL+D+VDA      S+ + S + S+ DVMTWLS+A+T HDTCTDGF ++ 
Sbjct: 139 VRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTNHDTCTDGFDEIE 198

Query: 211 DLA--VKDQVEAKLSDLSEMISNSLAIFSG--FGGGDLPVQNRRRRLMESGAQPWTAENG 270
                VKDQV   + DLSEM+SN LAIF+G       +PV N R+ L     +       
Sbjct: 199 GQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETEE------ 258

Query: 271 GDGGFPQWLSGKDRRLLASPISSIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIVIYI 324
                P WL  +DR LL +P S+IQADI V+KDGSG FKT+AEAI+ AP  S RR VIY+
Sbjct: 259 ----LPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYV 310

BLAST of HG10008896 vs. ExPASy Swiss-Prot
Match: Q43111 (Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 2.3e-27
Identity = 96/303 (31.68%), Postives = 149/303 (49.17%), Query Frame = 0

Query: 40  RSRVLIISFLSVFLIAASVVS-AAVLVRSRIASNQP---HFPHN--NPSQAISRACSHTL 99
           R R++II+  S+ LIA  + + A V++ +R + + P     P    +P+ ++   C  T 
Sbjct: 26  RKRLIIIAVSSIVLIAVIIAAVAGVVIHNRNSESSPSSDSVPQTELSPAASLKAVCDTTR 85

Query: 100 YPSLCLSSLLSFPGSLSADVHDLVHISLNLTLQHL----TKALYSTSQIPISQISNDPLA 159
           YPS C SS+ S P S + D   L  +SL + +  L    +K   +  Q    Q + D + 
Sbjct: 86  YPSSCFSSISSLPESNTTDPELLFKLSLRVAIDELSSFPSKLRANAEQDARLQKAID-VC 145

Query: 160 HSAYEDCMELLNDAVDAFS--LSLFSPEASNHDVMTWLSAALTYHDTCTDGFADVADLAV 219
            S + D ++ LND++ A        +  AS  +V TWLSAALT  DTC D   ++   A 
Sbjct: 146 SSVFGDALDRLNDSISALGTVAGRIASSASVSNVETWLSAALTDQDTCLDAVGELNSTAA 205

Query: 220 K---DQVEAKLSDLSEMISNSLAIFSGFGG----GDLPVQNRRRRLMESGAQPWTAENGG 279
           +    ++E  + + +E  SNSLAI +   G     + P+ +RR                 
Sbjct: 206 RGALQEIETAMRNSTEFASNSLAIVTKILGLLSRFETPIHHRRLL--------------- 265

Query: 280 DGGFPQWLSGKDRRLLASPISSIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIVIYIK 324
             GFP+WL   +RRLL    +    D VVAKDGSG+FKT+ EA++     S  R  +Y+K
Sbjct: 266 --GFPEWLGAAERRLLEEKNNDSTPDAVVAKDGSGQFKTIGEALKLVKKKSEERFSVYVK 310

BLAST of HG10008896 vs. ExPASy Swiss-Prot
Match: Q43143 (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 5.2e-27
Identity = 89/300 (29.67%), Postives = 159/300 (53.00%), Query Frame = 0

Query: 40  RSRVLIISFLSVFLIAASVVSAAVLVRSRIASNQPHFP----HNNPSQAISRACSHTLYP 99
           R + + ++ ++  L+ A+V+     V+S   ++  H       ++    +  ACS+TL+P
Sbjct: 17  RKKKIYLAIVASVLLVAAVIGVVAGVKSHSKNSDDHADIMAISSSAHAIVKSACSNTLHP 76

Query: 100 SLCLSSLLS---FPGSLSADVHDLVHISLNLTLQHLTKALYSTSQIPISQISNDPLAHSA 159
            LC S++++   F   +++   D++ +SLN+T++ + +  Y+  ++  ++    P    A
Sbjct: 77  ELCYSAIVNVSDFSKKVTSQ-KDVIELSLNITVKAVRRNYYAVKELIKTRKGLTPREKVA 136

Query: 160 YEDCMELLNDAVDAF-----SLSLFSPEAS----NHDVMTWLSAALTYHDTCTDGFA-DV 219
             DC+E +++ +D        L L+  + S      D+ T +S+A+T  +TC DGF+ D 
Sbjct: 137 LHDCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSAITNQETCLDGFSHDE 196

Query: 220 ADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDLPVQNRRRRLMESGAQPWTAENGGDG 279
           AD  V+  +      + +M SN+LA+       D  + N  +    +  +    +N   G
Sbjct: 197 ADKKVRKVLLKGQKHVEKMCSNALAMICNM--TDTDIANEMKLSAPANNRKLVEDN---G 256

Query: 280 GFPQWLSGKDRRLLASPISSIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIVIYIKAG 323
            +P+WLS  DRRLL S  S++  D+VVA DGSG +KTV+EA+  AP  S +R VI IKAG
Sbjct: 257 EWPEWLSAGDRRLLQS--STVTPDVVVAADGSGDYKTVSEAVRKAPEKSSKRYVIRIKAG 308

BLAST of HG10008896 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 116.7 bits (291), Expect = 4.9e-25
Identity = 98/310 (31.61%), Postives = 153/310 (49.35%), Query Frame = 0

Query: 36  SHLPRSRVLIISFLSV-FLIAASVVSAAVLVRSRIASNQPHFPHNNPSQAISRA-CSHTL 95
           S    ++ LI+S  ++  L+ AS+V  A    ++   NQ     ++ S AI ++ CS TL
Sbjct: 12  SDFKNNKKLILSSAAIALLLLASIVGIAATTTNQ-NKNQKITTLSSTSHAILKSVCSSTL 71

Query: 96  YPSLCLSSLLSFPGSLSADVHDLVHISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAY 155
           YP LC S++ +  G       +++  SLNLT + +    ++  ++   +    P   +A 
Sbjct: 72  YPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTAL 131

Query: 156 EDCMELLNDAVDAFSLSL---------FSPEASNHDVMTWLSAALTYHDTCTDGFA-DVA 215
            DC+E +++ +D   +++          S      D+ T +S+A+T   TC DGF+ D A
Sbjct: 132 HDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDA 191

Query: 216 DLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDLP-----------VQNRRRRLMESGAQ 275
           D  V+  +      +  M SN+LA+       D+              N  R+L E    
Sbjct: 192 DRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEV--- 251

Query: 276 PWTAENGGDGGFPQWLSGKDRRLLASPISSIQADIVVAKDGSGKFKTVAEAIEAAPSSSG 323
             T +   D G+P+WLS  DRRLL    S+I+AD  VA DGSG F TVA A+ AAP  S 
Sbjct: 252 --TGDLDSD-GWPKWLSVGDRRLLQG--STIKADATVADDGSGDFTTVAAAVAAAPEKSN 311

BLAST of HG10008896 vs. ExPASy TrEMBL
Match: A0A5D3C3J9 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00480 PE=3 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.4e-147
Identity = 280/324 (86.42%), Postives = 298/324 (91.98%), Query Frame = 0

Query: 3   NNNYNKLHSSRSVDSTPATSFHLSSNDPPPPSSSHLPRSRVLIISFLSVFLIAASVVSAA 62
           NNNY +LH+S S ++TPATSF LS NDPPPPSSS   RSR+LIISF+SV LIAASVVSAA
Sbjct: 4   NNNYKQLHTSHSANTTPATSFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAA 63

Query: 63  VLVRSRIASNQPHFPHNNPSQAISRACSHTLYPSLCLSSLLSFPGSLSADVHDLVHISLN 122
           +LVRSRIAS+QPHFPHN PSQAISRACSHTLYPSLCLSSLLSFPG+ SADVHDLVHISLN
Sbjct: 64  ILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHISLN 123

Query: 123 LTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSLSLFSPEASNHDVM 182
           LTLQHLTKALYSTSQIPI QIS DPLAHSAYEDCMELLNDA+DAFSLSLFS  ASNHD+M
Sbjct: 124 LTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNHDIM 183

Query: 183 TWLSAALTYHDTCTDGFADVADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDLPVQNR 242
           TWLSAALTYHDTCTDGF DV DL VK++VEAKLSDLSEMISNSLAIFSGFGGGDLPV+NR
Sbjct: 184 TWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENR 243

Query: 243 -RRRLMESGA-QPWTAENGGD-GGFPQWLSGKDRRLLASPISSIQADIVVAKDGSGKFKT 302
            RRRLMESG    W AENGGD  GFP+WLSGKDRRLLA+PIS+IQADIVVAKDGSGKFKT
Sbjct: 244 KRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGKFKT 303

Query: 303 VAEAIEAAPSSSGRRIVIYIKAGK 324
           VAEAIEAAPSSSGRRI+IYIKAGK
Sbjct: 304 VAEAIEAAPSSSGRRIIIYIKAGK 327

BLAST of HG10008896 vs. ExPASy TrEMBL
Match: A0A1S3AUZ6 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482964 PE=3 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.4e-147
Identity = 280/324 (86.42%), Postives = 298/324 (91.98%), Query Frame = 0

Query: 3   NNNYNKLHSSRSVDSTPATSFHLSSNDPPPPSSSHLPRSRVLIISFLSVFLIAASVVSAA 62
           NNNY +LH+S S ++TPATSF LS NDPPPPSSS   RSR+LIISF+SV LIAASVVSAA
Sbjct: 4   NNNYKQLHTSHSANTTPATSFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAA 63

Query: 63  VLVRSRIASNQPHFPHNNPSQAISRACSHTLYPSLCLSSLLSFPGSLSADVHDLVHISLN 122
           +LVRSRIAS+QPHFPHN PSQAISRACSHTLYPSLCLSSLLSFPG+ SADVHDLVHISLN
Sbjct: 64  ILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHISLN 123

Query: 123 LTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSLSLFSPEASNHDVM 182
           LTLQHLTKALYSTSQIPI QIS DPLAHSAYEDCMELLNDA+DAFSLSLFS  ASNHD+M
Sbjct: 124 LTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNHDIM 183

Query: 183 TWLSAALTYHDTCTDGFADVADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDLPVQNR 242
           TWLSAALTYHDTCTDGF DV DL VK++VEAKLSDLSEMISNSLAIFSGFGGGDLPV+NR
Sbjct: 184 TWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENR 243

Query: 243 -RRRLMESGA-QPWTAENGGD-GGFPQWLSGKDRRLLASPISSIQADIVVAKDGSGKFKT 302
            RRRLMESG    W AENGGD  GFP+WLSGKDRRLLA+PIS+IQADIVVAKDGSGKFKT
Sbjct: 244 KRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGKFKT 303

Query: 303 VAEAIEAAPSSSGRRIVIYIKAGK 324
           VAEAIEAAPSSSGRRI+IYIKAGK
Sbjct: 304 VAEAIEAAPSSSGRRIIIYIKAGK 327

BLAST of HG10008896 vs. ExPASy TrEMBL
Match: A0A0A0KJG1 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G139430 PE=3 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 1.2e-146
Identity = 275/323 (85.14%), Postives = 295/323 (91.33%), Query Frame = 0

Query: 3   NNNYNKLHSSRSVDSTPATSFHLSSNDPPPPSSSHLPRSRVLIISFLSVFLIAASVVSAA 62
           NN+YNKLH+S S ++TPA SF LS NDPPPPSSS   RSR+LIISF+SV LIAASVVSAA
Sbjct: 4   NNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAA 63

Query: 63  VLVRSRIASNQPHFPHNNPSQAISRACSHTLYPSLCLSSLLSFPGSLSADVHDLVHISLN 122
           +LVRS + S+QPHFPHN PSQAISRACSHTLYPSLCLSSLLSFPG+ SADVHDLVHISLN
Sbjct: 64  ILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHISLN 123

Query: 123 LTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSLSLFSPEASNHDVM 182
           LTLQHLTKALYSTSQIP+ QIS DPLAHSAYEDCMELLNDA+DAFSLSLFS +ASNHD+M
Sbjct: 124 LTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNHDIM 183

Query: 183 TWLSAALTYHDTCTDGFADVADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDLPVQNR 242
           TWLSAALTYHDTCT GF DVADL VKD+VEAKLSDLSEMISNSLAIFSGFGGGDLPV+NR
Sbjct: 184 TWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENR 243

Query: 243 -RRRLMESGAQPWTAENGGD-GGFPQWLSGKDRRLLASPISSIQADIVVAKDGSGKFKTV 302
            RRRLMES    W AENGGD  GFP WLSGKDRRLLA+P+S+IQADIVVAKDGSGKFKTV
Sbjct: 244 KRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTV 303

Query: 303 AEAIEAAPSSSGRRIVIYIKAGK 324
           AEAIEAAPSSSGRRI+IYIKAGK
Sbjct: 304 AEAIEAAPSSSGRRIIIYIKAGK 326

BLAST of HG10008896 vs. ExPASy TrEMBL
Match: A0A6J1IA52 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111471502 PE=3 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 4.3e-133
Identity = 267/329 (81.16%), Postives = 285/329 (86.63%), Query Frame = 0

Query: 2   INNNYNKLHSSRSVDSTPATSFHLSSN-----DPPPPSSSHLPRSRVLIISFLSVFLIAA 61
           +NNNYNKLHSSRS D TP TSF +S++      PPPPSSSH    R+L+ISF S+ LI A
Sbjct: 1   MNNNYNKLHSSRSGD-TPVTSFRVSNDHSNPPPPPPPSSSH---RRILLISFFSILLITA 60

Query: 62  SVVSAAVLVRSRI-ASNQPHFPHNNPSQAISRACSHTLYPSLCLSSLLSFPGSLSADVHD 121
           S VSA VLV SRI  S + HFPHN P+QAISRACS TLYPSLC+SSLLS PGSLSADVHD
Sbjct: 61  STVSAVVLVHSRITTSTESHFPHNKPTQAISRACSLTLYPSLCISSLLSAPGSLSADVHD 120

Query: 122 LVHISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSLSLFSPE 181
           LVHISLNLTLQHLTKALYSTSQIPISQISNDPLA SAYEDCMELLNDAV+AFSLSLFS  
Sbjct: 121 LVHISLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTT 180

Query: 182 ASNHDVMTWLSAALTYHDTCTDGFADVADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGG 241
           A+NHDVMTWLSAALTYHDTCTDGF+DVAD  VKDQ+ AKLSDLSEMISNSLAIFSGFGGG
Sbjct: 181 ATNHDVMTWLSAALTYHDTCTDGFSDVAD-PVKDQITAKLSDLSEMISNSLAIFSGFGGG 240

Query: 242 DLPVQN-RRRRLMESGAQPWTAENGGDGGFPQWLSGKDRRLLASPISSIQADIVVAKDGS 301
           DLPV+N RRRRLMES  Q    EN GDG FP+W+SGKDRRLLA+PISSIQADIVVAKDGS
Sbjct: 241 DLPVENRRRRRLMESDEQWLAEENNGDGDFPRWVSGKDRRLLAAPISSIQADIVVAKDGS 300

Query: 302 GKFKTVAEAIEAAPSSSGRRIVIYIKAGK 324
           GKFKTVAEAI AAPSSS RRIVIYIKAGK
Sbjct: 301 GKFKTVAEAIAAAPSSSSRRIVIYIKAGK 324

BLAST of HG10008896 vs. ExPASy TrEMBL
Match: A0A6J1E2V1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430323 PE=3 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 6.2e-132
Identity = 264/326 (80.98%), Postives = 283/326 (86.81%), Query Frame = 0

Query: 2   INNNYNKLHSSRSVDSTPATSFHLSS--NDPPPPSSSHLPRSRVLIISFLSVFLIAASVV 61
           +NNNYN LHSS S D TPA+SF +S+  +DP PP S    R R+L+ISF S+ LI AS V
Sbjct: 1   MNNNYNMLHSSHSGD-TPASSFRVSNDHSDPLPPPSPSSSRRRILLISFFSILLITASAV 60

Query: 62  SAAVLVRSRI-ASNQPHFPHNNPSQAISRACSHTLYPSLCLSSLLSFPGSLSADVHDLVH 121
           SA VLV SRI AS + HFPHN P+QAISRACS TLYPSLCLSSLLS PGSLSADVHDLVH
Sbjct: 61  SAVVLVHSRIAASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLVH 120

Query: 122 ISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSLSLFSPEASN 181
           ISLNLTLQHLTKALYSTSQIPISQISNDPLA SAYEDCMELLNDAV+AFSLSLFS  A+N
Sbjct: 121 ISLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTATN 180

Query: 182 HDVMTWLSAALTYHDTCTDGFADVADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDLP 241
           HDVMTWLSAALTYHDTCTDGF+DVAD  +KDQ+ AKLSDLSEMISNSLAIFSGFGG DLP
Sbjct: 181 HDVMTWLSAALTYHDTCTDGFSDVAD-PIKDQITAKLSDLSEMISNSLAIFSGFGGDDLP 240

Query: 242 VQN-RRRRLMESGAQPWTAENGGDGGFPQWLSGKDRRLLASPISSIQADIVVAKDGSGKF 301
           V+N RRRRLMES  Q    EN GDG FP+W+SGKDRRLLA+PISSIQADIVVAKDGSGKF
Sbjct: 241 VENRRRRRLMESDEQWLAEENNGDGDFPRWVSGKDRRLLAAPISSIQADIVVAKDGSGKF 300

Query: 302 KTVAEAIEAAPSSSGRRIVIYIKAGK 324
           KTVAEAI AAPSSSGRRIVIYIKAGK
Sbjct: 301 KTVAEAIAAAPSSSGRRIVIYIKAGK 324

BLAST of HG10008896 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 230.7 bits (587), Expect = 1.6e-60
Identity = 139/321 (43.30%), Postives = 201/321 (62.62%), Query Frame = 0

Query: 12  SRSVDSTPATSFHLSSNDPPPPSSSHLPRSRVLIISF-LSVFLIAASVVSAAVLVRSRIA 71
           S +  +T A   +  S    P +++   + ++++ S  L++ LI A+ + A V  R ++ 
Sbjct: 14  SVTTSTTTAPILNQVSTSEQPENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLN 73

Query: 72  SNQPHFPHNNPSQAISRACSHTLYPSLCLSSLLSFPGSLSA-DVHDLVHISLNLTLQHLT 131
            + P      PSQAIS+AC  T +P LC+ SL+ FPGSL+A    DL+H+++N+TL H +
Sbjct: 74  QSVPGLA-RKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFS 133

Query: 132 KALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFS---LSLFSPEASNHDVMTWLS 191
            ALYS++   +S +   P A SAY+ C+ELL+D+VDA S    S+ S  A   DV TWLS
Sbjct: 134 HALYSSAS--LSFVDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLS 193

Query: 192 AALTYHDTCTDGFADVADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGD----LPVQNR 251
           AALT HDTCT+GF  V D  VKD + A L +LSE++SN LAIFS    GD    +P+QNR
Sbjct: 194 AALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNR 253

Query: 252 RRRLMESGAQPWTAENGGDGGFPQWLSGKDRRLLASPISSIQADIVVAKDGSGKFKTVAE 311
           R   +E   +           FP+W+  K+R +L  P+S IQADI+V+KDG+G  KT++E
Sbjct: 254 RLLGVEEREEK----------FPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISE 313

Query: 312 AIEAAPSSSGRRIVIYIKAGK 324
           AI+ AP +S RRI+IY+KAG+
Sbjct: 314 AIKKAPQNSTRRIIIYVKAGR 321

BLAST of HG10008896 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 224.6 bits (571), Expect = 1.2e-58
Identity = 136/304 (44.74%), Postives = 184/304 (60.53%), Query Frame = 0

Query: 31  PPPSSSHLPRSRVLIISFLSVFLIAASVVSAAVLVRSRIASNQPHFPH--NNPSQAISRA 90
           P  S   L +     +   ++ ++   VV   +    R   +    P     P+QAISR 
Sbjct: 19  PATSLPELQKKTKTKLILFTLAVLVVGVVCFGIFAGIRAVDSGKTEPKLTRKPTQAISRT 78

Query: 91  CSHTLYPSLCLSSLLSFPGSLSADVHDLVHISLNLTLQHLTKALYSTSQIPISQISNDPL 150
           CS +LYP+LC+ +LL FPGSL+AD ++L+HIS N TLQ  +KALY++S I  +Q+   P 
Sbjct: 79  CSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSSTITYTQM--PPR 138

Query: 151 AHSAYEDCMELLNDAVDAF-----SLSLFSPEASNHDVMTWLSAALTYHDTCTDGFADVA 210
             SAY+ C+ELL+D+VDA      S+ + S + S+ DVMTWLS+A+T HDTCTDGF ++ 
Sbjct: 139 VRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTNHDTCTDGFDEIE 198

Query: 211 DLA--VKDQVEAKLSDLSEMISNSLAIFSG--FGGGDLPVQNRRRRLMESGAQPWTAENG 270
                VKDQV   + DLSEM+SN LAIF+G       +PV N R+ L     +       
Sbjct: 199 GQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETEE------ 258

Query: 271 GDGGFPQWLSGKDRRLLASPISSIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIVIYI 324
                P WL  +DR LL +P S+IQADI V+KDGSG FKT+AEAI+ AP  S RR VIY+
Sbjct: 259 ----LPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYV 310

BLAST of HG10008896 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 116.7 bits (291), Expect = 3.5e-26
Identity = 98/310 (31.61%), Postives = 153/310 (49.35%), Query Frame = 0

Query: 36  SHLPRSRVLIISFLSV-FLIAASVVSAAVLVRSRIASNQPHFPHNNPSQAISRA-CSHTL 95
           S    ++ LI+S  ++  L+ AS+V  A    ++   NQ     ++ S AI ++ CS TL
Sbjct: 12  SDFKNNKKLILSSAAIALLLLASIVGIAATTTNQ-NKNQKITTLSSTSHAILKSVCSSTL 71

Query: 96  YPSLCLSSLLSFPGSLSADVHDLVHISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAY 155
           YP LC S++ +  G       +++  SLNLT + +    ++  ++   +    P   +A 
Sbjct: 72  YPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTAL 131

Query: 156 EDCMELLNDAVDAFSLSL---------FSPEASNHDVMTWLSAALTYHDTCTDGFA-DVA 215
            DC+E +++ +D   +++          S      D+ T +S+A+T   TC DGF+ D A
Sbjct: 132 HDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDA 191

Query: 216 DLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDLP-----------VQNRRRRLMESGAQ 275
           D  V+  +      +  M SN+LA+       D+              N  R+L E    
Sbjct: 192 DRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEV--- 251

Query: 276 PWTAENGGDGGFPQWLSGKDRRLLASPISSIQADIVVAKDGSGKFKTVAEAIEAAPSSSG 323
             T +   D G+P+WLS  DRRLL    S+I+AD  VA DGSG F TVA A+ AAP  S 
Sbjct: 252 --TGDLDSD-GWPKWLSVGDRRLLQG--STIKADATVADDGSGDFTTVAAAVAAAPEKSN 311

BLAST of HG10008896 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 116.7 bits (291), Expect = 3.5e-26
Identity = 92/312 (29.49%), Postives = 156/312 (50.00%), Query Frame = 0

Query: 34  SSSHLPRSRVLIISFLSVFLIAASVVSAAVLVRSRIASNQPHFPHNNPSQAISRACSHTL 93
           S  +  +++ L++   +V L+  + V+      S+    +   P ++    +  +CS T 
Sbjct: 10  SKDNFKKNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSH--AVLRSSCSSTR 69

Query: 94  YPSLCLSSLLSFPGSLSADVHDLVHISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAY 153
           YP LC+S++++  G       D++  S+NLT+  +    ++  ++   +    P   +A 
Sbjct: 70  YPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTAL 129

Query: 154 EDCMELLNDAVDAF-----SLSLFSPEAS--NH--DVMTWLSAALTYHDTCTDGFA-DVA 213
            DC+E +++ +D        L L+  + +   H  D+ T +S+A+T  +TC DGF+ D A
Sbjct: 130 HDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDA 189

Query: 214 DLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDL-------PVQNRRRRLMESGAQPWTA 273
           D  V+  +      +  M SN+LA+       D+        + +  R+L E   +   A
Sbjct: 190 DKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVA 249

Query: 274 -ENGGDG-----GFPQWLSGKDRRLLASPISSIQADIVVAKDGSGKFKTVAEAIEAAPSS 323
            +  G G     G+P WLS  DRRLL    S ++AD  VA DGSG FKTVA A+ AAP +
Sbjct: 250 VDIAGAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAAAVAAAPEN 309

BLAST of HG10008896 vs. TAIR 10
Match: AT5G04960.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 107.5 bits (267), Expect = 2.1e-23
Identity = 90/300 (30.00%), Postives = 145/300 (48.33%), Query Frame = 0

Query: 25  LSSNDPPPPSSSHLPRSRVLIISFLSVFLIAASVVSAAVLVRSRIASNQPHFPHNNP--S 84
           L  ++     +S   + R+ II+  S+ L+   VV A V   +R  S +P   +N    S
Sbjct: 7   LDEHEQAKLEASRKTKKRIAIIAISSIVLVCI-VVGAVVGTTARDNSKKPPTENNGEPIS 66

Query: 85  QAISRACSHTLYPSLCLSSLLSFPGSLSADVHDLVHISLNLTLQHLTKALYSTSQIPISQ 144
            ++   C  TL+   C  +L S P +  +   +L   ++ +T+  L+K L   S    + 
Sbjct: 67  VSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLDGFS----NG 126

Query: 145 ISNDPLAHSAYEDCMELLNDAVDAFSLSLFSPEASNHDVMTWLSAALTYHDTCTDGFADV 204
              D    +A   C+EL+  AVD  + ++ S   +  D+ TWLS+  TY +TC D   + 
Sbjct: 127 EHMDNATSAAMGACVELIGLAVDQLNETMTSSLKNFDDLRTWLSSVGTYQETCMDALVEA 186

Query: 205 ADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDLPVQNRRRRLMESGAQPWTAENGGDG 264
              ++    E  L + +EM SN+LAI +  G     V+ RRRRL+E+G       +    
Sbjct: 187 NKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVVVAD---- 246

Query: 265 GFPQWLSGKDRRLLASPISSIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIVIYIKAG 323
             P     + RRLL S     +A IVVAKDGSGK++T+ EA+      + +  +IY+K G
Sbjct: 247 -LPMM---EGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKG 293