HG10008724 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10008724 vs. NCBI nr
Match: XP_016903211.1 (PREDICTED: E3 ubiquitin-protein ligase RGLG2-like isoform X3 [Cucumis melo])

HSP 1 Score: 828.9 bits (2140), Expect = 2.0e-236
Identity = 415/439 (94.53%), Postives = 421/439 (95.90%), Query Frame = 0

Query: 5   LQSSCEVMGGKSSKHGSSRRILSSRSNSQQWSQYGYPQSPYDQSRSTPQYRYAPPTPSYG 64
           L SSCEVMGGKSSKHGSSRR  SSRSNSQQW  YGYP+SPY QSRSTP  RYAPPTPSYG
Sbjct: 16  LFSSCEVMGGKSSKHGSSRRNSSSRSNSQQWGHYGYPESPYTQSRSTP--RYAPPTPSYG 75

Query: 65  GTQAPETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSF 124
           GTQAPETRKRLERKYSRIDDNYNSL+QVTEALARAGLESSNLIVGIDFTKSNEWTGARSF
Sbjct: 76  GTQAPETRKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSF 135

Query: 125 NRRSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYPDERFC 184
           NRRSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFY DERFC
Sbjct: 136 NRRSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFC 195

Query: 185 NGFEEVLGRYRELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDT 244
           NGFEEVLGRY+ELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDT
Sbjct: 196 NGFEEVLGRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDT 255

Query: 245 ERGQFSPQEKKTIEAIVKASEYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFT 304
           ERGQFSPQEKKTIEAIVKAS+YPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFT
Sbjct: 256 ERGQFSPQEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFT 315

Query: 305 NIMLKNMDRSRKETEFALAALMEIPSQYKATLELDILGASRGKAIDRIPLPPPRYGPSTQ 364
           NIMLKN+DRSRKE EFALAALMEIPSQYKATLEL +LG SRGKAIDRIPLPPP YGPS Q
Sbjct: 316 NIMLKNIDRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSIQ 375

Query: 365 KTSNSSSSRPIPTSFGGVAPARTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQ 424
           KTSNSSSSRP P SF GVAP RTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQ
Sbjct: 376 KTSNSSSSRPTPPSFSGVAPVRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQ 435

Query: 425 DLQLCPICRSFIDTRIKLY 444
           DLQLCPICRSFIDTRIKLY
Sbjct: 436 DLQLCPICRSFIDTRIKLY 452

BLAST of HG10008724 vs. NCBI nr
Match: XP_016903210.1 (PREDICTED: E3 ubiquitin-protein ligase RGLG2-like isoform X2 [Cucumis melo])

HSP 1 Score: 828.2 bits (2138), Expect = 3.4e-236
Identity = 414/437 (94.74%), Postives = 420/437 (96.11%), Query Frame = 0

Query: 7   SSCEVMGGKSSKHGSSRRILSSRSNSQQWSQYGYPQSPYDQSRSTPQYRYAPPTPSYGGT 66
           SSCEVMGGKSSKHGSSRR  SSRSNSQQW  YGYP+SPY QSRSTP  RYAPPTPSYGGT
Sbjct: 28  SSCEVMGGKSSKHGSSRRNSSSRSNSQQWGHYGYPESPYTQSRSTP--RYAPPTPSYGGT 87

Query: 67  QAPETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNR 126
           QAPETRKRLERKYSRIDDNYNSL+QVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNR
Sbjct: 88  QAPETRKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNR 147

Query: 127 RSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYPDERFCNG 186
           RSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFY DERFCNG
Sbjct: 148 RSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNG 207

Query: 187 FEEVLGRYRELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTER 246
           FEEVLGRY+ELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTER
Sbjct: 208 FEEVLGRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTER 267

Query: 247 GQFSPQEKKTIEAIVKASEYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNI 306
           GQFSPQEKKTIEAIVKAS+YPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNI
Sbjct: 268 GQFSPQEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNI 327

Query: 307 MLKNMDRSRKETEFALAALMEIPSQYKATLELDILGASRGKAIDRIPLPPPRYGPSTQKT 366
           MLKN+DRSRKE EFALAALMEIPSQYKATLEL +LG SRGKAIDRIPLPPP YGPS QKT
Sbjct: 328 MLKNIDRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSIQKT 387

Query: 367 SNSSSSRPIPTSFGGVAPARTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDL 426
           SNSSSSRP P SF GVAP RTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDL
Sbjct: 388 SNSSSSRPTPPSFSGVAPVRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDL 447

Query: 427 QLCPICRSFIDTRIKLY 444
           QLCPICRSFIDTRIKLY
Sbjct: 448 QLCPICRSFIDTRIKLY 462

BLAST of HG10008724 vs. NCBI nr
Match: XP_016903209.1 (PREDICTED: E3 ubiquitin-protein ligase RGLG2-like isoform X1 [Cucumis melo])

HSP 1 Score: 828.2 bits (2138), Expect = 3.4e-236
Identity = 414/437 (94.74%), Postives = 420/437 (96.11%), Query Frame = 0

Query: 7   SSCEVMGGKSSKHGSSRRILSSRSNSQQWSQYGYPQSPYDQSRSTPQYRYAPPTPSYGGT 66
           SSCEVMGGKSSKHGSSRR  SSRSNSQQW  YGYP+SPY QSRSTP  RYAPPTPSYGGT
Sbjct: 32  SSCEVMGGKSSKHGSSRRNSSSRSNSQQWGHYGYPESPYTQSRSTP--RYAPPTPSYGGT 91

Query: 67  QAPETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNR 126
           QAPETRKRLERKYSRIDDNYNSL+QVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNR
Sbjct: 92  QAPETRKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNR 151

Query: 127 RSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYPDERFCNG 186
           RSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFY DERFCNG
Sbjct: 152 RSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNG 211

Query: 187 FEEVLGRYRELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTER 246
           FEEVLGRY+ELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTER
Sbjct: 212 FEEVLGRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTER 271

Query: 247 GQFSPQEKKTIEAIVKASEYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNI 306
           GQFSPQEKKTIEAIVKAS+YPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNI
Sbjct: 272 GQFSPQEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNI 331

Query: 307 MLKNMDRSRKETEFALAALMEIPSQYKATLELDILGASRGKAIDRIPLPPPRYGPSTQKT 366
           MLKN+DRSRKE EFALAALMEIPSQYKATLEL +LG SRGKAIDRIPLPPP YGPS QKT
Sbjct: 332 MLKNIDRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSIQKT 391

Query: 367 SNSSSSRPIPTSFGGVAPARTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDL 426
           SNSSSSRP P SF GVAP RTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDL
Sbjct: 392 SNSSSSRPTPPSFSGVAPVRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDL 451

Query: 427 QLCPICRSFIDTRIKLY 444
           QLCPICRSFIDTRIKLY
Sbjct: 452 QLCPICRSFIDTRIKLY 466

BLAST of HG10008724 vs. NCBI nr
Match: XP_038878428.1 (E3 ubiquitin-protein ligase RGLG2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 820.1 bits (2117), Expect = 9.3e-234
Identity = 410/432 (94.91%), Postives = 419/432 (96.99%), Query Frame = 0

Query: 12  MGGKSSKHGSSRRILSSRSNSQQWSQYGYPQSPYDQSRSTPQYRYAPPTPSYGGTQAPET 71
           MGG+SSKH SSRR  SSRSNSQQ +   YPQSPY QSRSTPQ+ YAPPTPSYGGTQAPE+
Sbjct: 1   MGGRSSKHVSSRRSSSSRSNSQQGTYGYYPQSPYAQSRSTPQHLYAPPTPSYGGTQAPES 60

Query: 72  RKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 131
           RKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH
Sbjct: 61  RKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 120

Query: 132 IGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYPDERFCNGFEEVL 191
           IGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYPDERFCNGFEEVL
Sbjct: 121 IGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYPDERFCNGFEEVL 180

Query: 192 GRYRELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 251
           GRYRELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP
Sbjct: 181 GRYRELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 240

Query: 252 QEKKTIEAIVKASEYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNM 311
           QEKKTIE+IVKASEYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNM
Sbjct: 241 QEKKTIESIVKASEYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNM 300

Query: 312 DRSRKETEFALAALMEIPSQYKATLELDILGASRGKAIDRIPLPPPRYGPSTQKTSNSSS 371
           DR+RKETEFALAALMEIPSQYKATLELDILGASRGK IDR+PLPPPRYGPSTQKTSNSSS
Sbjct: 301 DRARKETEFALAALMEIPSQYKATLELDILGASRGKEIDRVPLPPPRYGPSTQKTSNSSS 360

Query: 372 SRPIPTSFGGVAPARTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 431
           SRP+  SFGGV PA+TAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI
Sbjct: 361 SRPMQPSFGGVVPAQTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 420

Query: 432 CRSFIDTRIKLY 444
           CRSFIDTRIKLY
Sbjct: 421 CRSFIDTRIKLY 432

BLAST of HG10008724 vs. NCBI nr
Match: XP_008464499.1 (PREDICTED: E3 ubiquitin-protein ligase RGLG2-like isoform X4 [Cucumis melo] >XP_008464500.1 PREDICTED: E3 ubiquitin-protein ligase RGLG2-like isoform X4 [Cucumis melo] >XP_008464501.1 PREDICTED: E3 ubiquitin-protein ligase RGLG2-like isoform X4 [Cucumis melo] >XP_016903213.1 PREDICTED: E3 ubiquitin-protein ligase RGLG2-like isoform X4 [Cucumis melo] >XP_016903214.1 PREDICTED: E3 ubiquitin-protein ligase RGLG2-like isoform X4 [Cucumis melo] >KAA0057810.1 E3 ubiquitin-protein ligase RGLG2-like isoform X3 [Cucumis melo var. makuwa] >TYJ98494.1 E3 ubiquitin-protein ligase RGLG2-like isoform X3 [Cucumis melo var. makuwa])

HSP 1 Score: 818.5 bits (2113), Expect = 2.7e-233
Identity = 409/432 (94.68%), Postives = 415/432 (96.06%), Query Frame = 0

Query: 12  MGGKSSKHGSSRRILSSRSNSQQWSQYGYPQSPYDQSRSTPQYRYAPPTPSYGGTQAPET 71
           MGGKSSKHGSSRR  SSRSNSQQW  YGYP+SPY QSRSTP  RYAPPTPSYGGTQAPET
Sbjct: 1   MGGKSSKHGSSRRNSSSRSNSQQWGHYGYPESPYTQSRSTP--RYAPPTPSYGGTQAPET 60

Query: 72  RKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 131
           RKRLERKYSRIDDNYNSL+QVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH
Sbjct: 61  RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 120

Query: 132 IGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYPDERFCNGFEEVL 191
           IGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFY DERFCNGFEEVL
Sbjct: 121 IGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL 180

Query: 192 GRYRELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 251
           GRY+ELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP
Sbjct: 181 GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 240

Query: 252 QEKKTIEAIVKASEYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNM 311
           QEKKTIEAIVKAS+YPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKN+
Sbjct: 241 QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI 300

Query: 312 DRSRKETEFALAALMEIPSQYKATLELDILGASRGKAIDRIPLPPPRYGPSTQKTSNSSS 371
           DRSRKE EFALAALMEIPSQYKATLEL +LG SRGKAIDRIPLPPP YGPS QKTSNSSS
Sbjct: 301 DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSIQKTSNSSS 360

Query: 372 SRPIPTSFGGVAPARTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 431
           SRP P SF GVAP RTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI
Sbjct: 361 SRPTPPSFSGVAPVRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 420

Query: 432 CRSFIDTRIKLY 444
           CRSFIDTRIKLY
Sbjct: 421 CRSFIDTRIKLY 430

BLAST of HG10008724 vs. ExPASy Swiss-Prot
Match: Q9LY87 (E3 ubiquitin-protein ligase RGLG2 OS=Arabidopsis thaliana OX=3702 GN=RGLG2 PE=1 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 3.4e-162
Identity = 302/458 (65.94%), Postives = 345/458 (75.33%), Query Frame = 0

Query: 15  KSSKHGSSRRILSSRSNSQQWSQYG-----YPQSP----------------YDQSRSTPQ 74
           +S+   S+    SS ++ Q + QYG     YP  P                  Q  S P 
Sbjct: 16  RSTSASSASPSSSSWASQQSYPQYGAESYNYPPPPSYAQPPEYTQPPPPLYSTQPYSAPS 75

Query: 75  YRYAPPTPSYGGTQAPETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFT 134
           Y  APP+ SYG     + +KRLERKYS+I D+Y+SLEQVTEALARAGLESSNLIVGIDFT
Sbjct: 76  YS-APPSQSYGS----DNKKRLERKYSKISDDYSSLEQVTEALARAGLESSNLIVGIDFT 135

Query: 135 KSNEWTGARSFNRRSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQE 194
           KSNEWTGARSFNR+SLH IG   NPYEQAI IIG+TL++FDEDN+IPC+GFGDASTHDQ+
Sbjct: 136 KSNEWTGARSFNRKSLHFIGSSPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQD 195

Query: 195 VFSFYPDERFCNGFEEVLGRYRELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVII 254
           VFSF  ++RFCNGFEEVL RY+E+VP L+LAGPTSFAPII+MA+TIVEQSGGQYHVLVII
Sbjct: 196 VFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQSGGQYHVLVII 255

Query: 255 ADGQVTRSVDTERGQFSPQEKKTIEAIVKASEYPLSIILVGVGDGPWDMMREFDDNIPAR 314
           ADGQVTRSVDTE GQ SPQE+KT++AIV+AS+ PLSI+LVGVGDGPWDMMREFDDNIPAR
Sbjct: 256 ADGQVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWDMMREFDDNIPAR 315

Query: 315 AFDNFQFVNFTNIMLKNMDRSRKETEFALAALMEIPSQYKATLELDILGASRGKAIDRIP 374
           AFDNFQFVNFT IM KN  +S KETEFAL+ALMEIP QYKAT+EL++LG   G   +R P
Sbjct: 316 AFDNFQFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIELNLLGRRNGYIPERFP 375

Query: 375 LPPPRYGPSTQKTSNSSSSRPI--------PTSFGGVAPARTAPPLSSVSDAHACPICIS 434
           LPPP  G S+   S   S  P         PT    V  +   PP SS SD   CPIC+S
Sbjct: 376 LPPPMRGGSSSYNSPKPSRLPSFKPSVPPHPTEGYHVRSSPVPPPTSSASDNQLCPICLS 435

Query: 435 NAKDMAFGCGHQTCCECGQDLQLCPICRSFIDTRIKLY 444
           N KDMAFGCGHQTCCECG DLQ+CPICR+ I TRIKLY
Sbjct: 436 NPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKLY 468

BLAST of HG10008724 vs. ExPASy Swiss-Prot
Match: Q9SS90 (E3 ubiquitin-protein ligase RGLG1 OS=Arabidopsis thaliana OX=3702 GN=RGLG1 PE=1 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 3.5e-159
Identity = 288/411 (70.07%), Postives = 329/411 (80.05%), Query Frame = 0

Query: 36  SQYG--YPQSPYDQSRSTPQYRYAPPTPSYGGTQAPETRKRLERKYSRIDDNYNSLEQVT 95
           S YG  Y Q  Y  + S P Y   PP PS         RK+ +R+YS+I DNY+SL QV+
Sbjct: 91  SSYGHQYSQEGYASAASQPNY---PPPPSQSQV---ADRKKFDRRYSKISDNYSSLLQVS 150

Query: 96  EALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIGDEQNPYEQAIAIIGQTLSSF 155
           EAL RAGLESSNLIVGIDFTKSNEWTGA+SFNR+SLHH+ +  NPYEQAI IIG+TL++F
Sbjct: 151 EALGRAGLESSNLIVGIDFTKSNEWTGAKSFNRKSLHHLSNTPNPYEQAITIIGRTLAAF 210

Query: 156 DEDNMIPCFGFGDASTHDQEVFSFYPDERFCNGFEEVLGRYRELVPHLRLAGPTSFAPII 215
           DEDN+IPC+GFGDASTHDQ+VFSFYP+ RFCNGFEEVL RYRE+VP L+LAGPTSFAPII
Sbjct: 211 DEDNLIPCYGFGDASTHDQDVFSFYPEGRFCNGFEEVLARYREIVPQLKLAGPTSFAPII 270

Query: 216 EMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSPQEKKTIEAIVKASEYPLSIILV 275
           EMA+T+VEQS GQYHVLVIIADGQVTRSVDTE G+ SPQE+KT++AIVKAS  PLSI+LV
Sbjct: 271 EMAMTVVEQSSGQYHVLVIIADGQVTRSVDTEHGRLSPQEQKTVDAIVKASTLPLSIVLV 330

Query: 276 GVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNMDRSRKETEFALAALMEIPSQYK 335
           GVGDGPWDMM+EFDDNIPARAFDNFQFVNFT IM KN D+SRKETEFAL+ALMEIP QYK
Sbjct: 331 GVGDGPWDMMQEFDDNIPARAFDNFQFVNFTEIMSKNKDQSRKETEFALSALMEIPPQYK 390

Query: 336 ATLELDILGASRGKAIDRIPLPPPRYGPSTQKTSNSSSSRPIPTSFGGVAP-ARTAPPLS 395
           AT+EL++LG   G    RIPLPPP      Q  S+ SSSR IP     V P    +  +S
Sbjct: 391 ATIELNLLGVRNGNIPQRIPLPPP-----VQSGSSFSSSR-IPNFEPSVPPYPFESKQMS 450

Query: 396 SVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIDTRIKLY 444
           S  D   CPIC+SN K+MAFGCGHQTCCECG DL++CPICR+ I TRIKLY
Sbjct: 451 SADDIQLCPICLSNPKNMAFGCGHQTCCECGPDLKVCPICRAPIQTRIKLY 489

BLAST of HG10008724 vs. ExPASy Swiss-Prot
Match: Q8LB88 (E3 ubiquitin-protein ligase RGLG5 OS=Arabidopsis thaliana OX=3702 GN=RGLG5 PE=1 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 2.1e-151
Identity = 285/443 (64.33%), Postives = 336/443 (75.85%), Query Frame = 0

Query: 12  MGGKSSKH-----GSSRRILSSRSNSQQWSQYGYPQSPYDQSRSTPQYRYAPPTPSYGGT 71
           MGG SSK      GS RR   S S S  +S   + QS Y Q   TP +  A P  SY   
Sbjct: 1   MGGSSSKESPRGGGSGRRYERSVSGSSSYSS-AWDQSSYYQ---TPNHPSASPVSSYNSG 60

Query: 72  QAPETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNR 131
           +  +T K LERKYSRI DNY S+++VT AL+ AGLESSNLIVGID TKSNEWTGARSF R
Sbjct: 61  R--QTPKNLERKYSRIADNYRSIDEVTAALSHAGLESSNLIVGIDVTKSNEWTGARSFGR 120

Query: 132 RSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYPDERFCNG 191
           +SLH IG   NPY+QAI+IIG+TLS FDEDN+IPC+GFGDA+THDQ+VFSF P++ +CNG
Sbjct: 121 KSLHFIGTTPNPYQQAISIIGKTLSVFDEDNLIPCYGFGDATTHDQDVFSFNPNDTYCNG 180

Query: 192 FEEVLGRYRELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTER 251
           FEEVL  YRE+VP LRL+GPTSFAPIIE A+TIVE+SGGQYHVL+IIADGQVTRSVDT+ 
Sbjct: 181 FEEVLMCYREIVPQLRLSGPTSFAPIIERAMTIVEESGGQYHVLLIIADGQVTRSVDTDN 240

Query: 252 GQFSPQEKKTIEAIVKASEYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNI 311
           G FSPQE++TI+AIV+ASEYPLSI+LVGVGDGPWD MR+FDDNIPARAFDNFQFVNFT+I
Sbjct: 241 GGFSPQEQQTIDAIVRASEYPLSIVLVGVGDGPWDTMRQFDDNIPARAFDNFQFVNFTDI 300

Query: 312 MLKNMDRSRKETEFALAALMEIPSQYKATLELDILGASRGKAIDRIPLPPPRYGP----S 371
           M KN+D +RKE EFAL+ALMEIPSQYKATLEL +LG   G   DRI LPPP Y      +
Sbjct: 301 MSKNIDPARKEAEFALSALMEIPSQYKATLELGLLGQRTGHCPDRIALPPPTYATQSMRN 360

Query: 372 TQKTSNSSS--SRPIPTSFGGVAPARTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCC 431
           + +TS S+S  ++P         P+ T     + S    CP+C+ +AK+MAF CGHQTC 
Sbjct: 361 SPRTSRSTSFQNKPYDNGVSSTPPSTT----HNESQQQFCPVCLVSAKNMAFNCGHQTCA 420

Query: 432 ECGQDLQLCPICRSFIDTRIKLY 444
            CG+DL +CPICRS I  RIKLY
Sbjct: 421 GCGEDLHVCPICRSSISVRIKLY 433

BLAST of HG10008724 vs. ExPASy Swiss-Prot
Match: Q8RX26 (E3 ubiquitin-protein ligase RGLG3 OS=Arabidopsis thaliana OX=3702 GN=RGLG3 PE=1 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 2.1e-119
Identity = 220/364 (60.44%), Postives = 265/364 (72.80%), Query Frame = 0

Query: 80  SRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIGDEQNPY 139
           S I D+++SL+QV  +L  AGLESSNLI+GIDFTKSNEWTG  SFNR+SLH IG  QNPY
Sbjct: 12  SYIADHFSSLDQVITSLREAGLESSNLILGIDFTKSNEWTGRYSFNRKSLHAIGKRQNPY 71

Query: 140 EQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYPDERFCNGFEEVLGRYRELVP 199
           E+AI+IIG+TLS FDED++IPCFGFGD +T DQ VFSFYP+ + C+G E  + RYRE+VP
Sbjct: 72  EKAISIIGRTLSPFDEDDLIPCFGFGDVTTRDQYVFSFYPENKSCDGLENAVKRYREIVP 131

Query: 200 HLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSPQEKKTIEA 259
           HL+L+GPTSFAP+I+ AI IVEQ+  QYHVLVIIADGQVTR+ D   G+ SPQE+ T+ +
Sbjct: 132 HLKLSGPTSFAPVIDAAINIVEQNNMQYHVLVIIADGQVTRNPDVPLGRLSPQEEATMNS 191

Query: 260 IVKASEYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNMDRSRKETE 319
           I+ AS YPLSI+LVGVGDGPWD M++FDDNIP R FDNFQFVNFT IM ++ D ++KE  
Sbjct: 192 IMAASHYPLSIVLVGVGDGPWDTMKQFDDNIPHREFDNFQFVNFTKIMSEHKDAAKKEAA 251

Query: 320 FALAALMEIPSQYKATLELDILGASRGKAIDRIPLPPPRYGPSTQKTSNSSSSRPIPTSF 379
           FALAALMEIP QYKATL L+     R       PLPPP   P   +  N+  S P   + 
Sbjct: 252 FALAALMEIPFQYKATLSLN-RKPVRSSHQHHKPLPPP---PEVIERDNAVRSVPNQMTE 311

Query: 380 GGVAPARTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIDTR 439
                 R AP     S    CPIC++N KDMAF CGH TC ECG  +  CP+CR  I TR
Sbjct: 312 TAEKSDRLAP-----STVPVCPICLTNPKDMAFSCGHTTCKECGVVITTCPLCRQPITTR 366

Query: 440 IKLY 444
           I+LY
Sbjct: 372 IRLY 366

BLAST of HG10008724 vs. ExPASy Swiss-Prot
Match: Q9SAL0 (E3 ubiquitin-protein ligase RGLG4 OS=Arabidopsis thaliana OX=3702 GN=RGLG4 PE=1 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.4e-118
Identity = 215/406 (52.96%), Postives = 279/406 (68.72%), Query Frame = 0

Query: 39  GYPQSPYDQSRSTPQYRYAPPTPSYGGTQAPETRKRLERKYSRIDDNYNSLEQVTEALAR 98
           G  +S  + +  T   +   P+PS       +     ++KY+ I D ++SL+QV++AL  
Sbjct: 13  GKSRSSRNMTLGTTSSQSHEPSPSDPSLSLADNTNATKKKYALIPDRFSSLDQVSKALRE 72

Query: 99  AGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNM 158
           AGLESSNLI+G+DFTKSNEWTG  SF+ + LH +G+  NPYE+AI +IGQTL+ FDEDN+
Sbjct: 73  AGLESSNLILGVDFTKSNEWTGKTSFDGKCLHALGETSNPYEKAIFVIGQTLAPFDEDNL 132

Query: 159 IPCFGFGDASTHDQEVFSFYPDERFCNGFEEVLGRYRELVPHLRLAGPTSFAPIIEMAIT 218
           IPCFGFGD++THD+EVF F+ D   C+GFEEVL  Y+ + P+LRL+GPTS+ P+I+ A+ 
Sbjct: 133 IPCFGFGDSTTHDEEVFGFHSDNSPCHGFEEVLACYKRIAPNLRLSGPTSYGPLIDAAVD 192

Query: 219 IVEQSGGQYHVLVIIADGQVTRSVDTERGQFSPQEKKTIEAIVKASEYPLSIILVGVGDG 278
           IVE++ GQ+HVLVI+ADGQVTR  D   G+ S QEK TI+AIV AS Y LSI+LVGVGDG
Sbjct: 193 IVEKNNGQFHVLVIVADGQVTRGTDMAEGELSQQEKTTIDAIVNASSYALSIVLVGVGDG 252

Query: 279 PWDMMREFDDNIPARAFDNFQFVNFTNIMLKNMDRSRKETEFALAALMEIPSQYKATLEL 338
           PW+ MR+FDD IP R FDNFQFVNFT IM +N   S KET FALAALMEIP QY+A +EL
Sbjct: 253 PWEDMRKFDDKIPKREFDNFQFVNFTEIMTRNSPESAKETAFALAALMEIPFQYQAAIEL 312

Query: 339 DILGASRGKAIDRIPLPPP-RYGPSTQKTSNSSSSRPIPTSFGGVAPARTAPPLSSVSDA 398
            +LG   G A   +P PPP  Y P T        +  +P++    +P +T          
Sbjct: 313 RLLGKQTGLAKTIVPRPPPIPYTPPT--------NAELPSTASPASPEQT---------- 372

Query: 399 HACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIDTRIKLY 444
            +CPIC++N KD+AF CGH TC +CG  +  CPICR  I  R+KLY
Sbjct: 373 QSCPICLTNRKDVAFSCGHMTCGDCGSKISNCPICRVRITNRLKLY 400

BLAST of HG10008724 vs. ExPASy TrEMBL
Match: A0A0A0LP13 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G002920 PE=4 SV=1)

HSP 1 Score: 828.9 bits (2140), Expect = 9.7e-237
Identity = 413/439 (94.08%), Postives = 421/439 (95.90%), Query Frame = 0

Query: 5   LQSSCEVMGGKSSKHGSSRRILSSRSNSQQWSQYGYPQSPYDQSRSTPQYRYAPPTPSYG 64
           L SSCEVMGGKSSKH SSRR  SSRSNSQQWS YGYP+SPY QSRSTPQY YAPPTPSYG
Sbjct: 37  LFSSCEVMGGKSSKHRSSRRNSSSRSNSQQWSHYGYPESPYTQSRSTPQYEYAPPTPSYG 96

Query: 65  GTQAPETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSF 124
           GTQAPETRKRLERKYSRIDDNYNSL+QVTEALARAGLESSNLIVGIDFTKSNEWTGARSF
Sbjct: 97  GTQAPETRKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSF 156

Query: 125 NRRSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYPDERFC 184
           NRRSLHHIG+EQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFY DERFC
Sbjct: 157 NRRSLHHIGEEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFC 216

Query: 185 NGFEEVLGRYRELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDT 244
           NGFEEVLGRY+ELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDT
Sbjct: 217 NGFEEVLGRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDT 276

Query: 245 ERGQFSPQEKKTIEAIVKASEYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFT 304
           ERGQFSPQEKKTIEAIVKAS+YPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFT
Sbjct: 277 ERGQFSPQEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFT 336

Query: 305 NIMLKNMDRSRKETEFALAALMEIPSQYKATLELDILGASRGKAIDRIPLPPPRYGPSTQ 364
           NIMLKN+DRSRKE EFALAALMEIPSQYKATLEL +LG SRGKAIDRIPLPPP YGPS +
Sbjct: 337 NIMLKNIDRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSVR 396

Query: 365 KTSNSSSSRPIPTSFGGVAPARTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQ 424
           KTSNSSSSRP P SF G AP RTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQ
Sbjct: 397 KTSNSSSSRPTPPSFSGGAPGRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQ 456

Query: 425 DLQLCPICRSFIDTRIKLY 444
           DLQLCPICRSFIDTRIKLY
Sbjct: 457 DLQLCPICRSFIDTRIKLY 475

BLAST of HG10008724 vs. ExPASy TrEMBL
Match: A0A1S4E4Q2 (E3 ubiquitin-protein ligase RGLG2-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103502363 PE=4 SV=1)

HSP 1 Score: 828.9 bits (2140), Expect = 9.7e-237
Identity = 415/439 (94.53%), Postives = 421/439 (95.90%), Query Frame = 0

Query: 5   LQSSCEVMGGKSSKHGSSRRILSSRSNSQQWSQYGYPQSPYDQSRSTPQYRYAPPTPSYG 64
           L SSCEVMGGKSSKHGSSRR  SSRSNSQQW  YGYP+SPY QSRSTP  RYAPPTPSYG
Sbjct: 16  LFSSCEVMGGKSSKHGSSRRNSSSRSNSQQWGHYGYPESPYTQSRSTP--RYAPPTPSYG 75

Query: 65  GTQAPETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSF 124
           GTQAPETRKRLERKYSRIDDNYNSL+QVTEALARAGLESSNLIVGIDFTKSNEWTGARSF
Sbjct: 76  GTQAPETRKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSF 135

Query: 125 NRRSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYPDERFC 184
           NRRSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFY DERFC
Sbjct: 136 NRRSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFC 195

Query: 185 NGFEEVLGRYRELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDT 244
           NGFEEVLGRY+ELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDT
Sbjct: 196 NGFEEVLGRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDT 255

Query: 245 ERGQFSPQEKKTIEAIVKASEYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFT 304
           ERGQFSPQEKKTIEAIVKAS+YPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFT
Sbjct: 256 ERGQFSPQEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFT 315

Query: 305 NIMLKNMDRSRKETEFALAALMEIPSQYKATLELDILGASRGKAIDRIPLPPPRYGPSTQ 364
           NIMLKN+DRSRKE EFALAALMEIPSQYKATLEL +LG SRGKAIDRIPLPPP YGPS Q
Sbjct: 316 NIMLKNIDRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSIQ 375

Query: 365 KTSNSSSSRPIPTSFGGVAPARTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQ 424
           KTSNSSSSRP P SF GVAP RTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQ
Sbjct: 376 KTSNSSSSRPTPPSFSGVAPVRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQ 435

Query: 425 DLQLCPICRSFIDTRIKLY 444
           DLQLCPICRSFIDTRIKLY
Sbjct: 436 DLQLCPICRSFIDTRIKLY 452

BLAST of HG10008724 vs. ExPASy TrEMBL
Match: A0A1S4E4Q6 (E3 ubiquitin-protein ligase RGLG2-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502363 PE=4 SV=1)

HSP 1 Score: 828.2 bits (2138), Expect = 1.7e-236
Identity = 414/437 (94.74%), Postives = 420/437 (96.11%), Query Frame = 0

Query: 7   SSCEVMGGKSSKHGSSRRILSSRSNSQQWSQYGYPQSPYDQSRSTPQYRYAPPTPSYGGT 66
           SSCEVMGGKSSKHGSSRR  SSRSNSQQW  YGYP+SPY QSRSTP  RYAPPTPSYGGT
Sbjct: 32  SSCEVMGGKSSKHGSSRRNSSSRSNSQQWGHYGYPESPYTQSRSTP--RYAPPTPSYGGT 91

Query: 67  QAPETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNR 126
           QAPETRKRLERKYSRIDDNYNSL+QVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNR
Sbjct: 92  QAPETRKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNR 151

Query: 127 RSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYPDERFCNG 186
           RSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFY DERFCNG
Sbjct: 152 RSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNG 211

Query: 187 FEEVLGRYRELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTER 246
           FEEVLGRY+ELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTER
Sbjct: 212 FEEVLGRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTER 271

Query: 247 GQFSPQEKKTIEAIVKASEYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNI 306
           GQFSPQEKKTIEAIVKAS+YPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNI
Sbjct: 272 GQFSPQEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNI 331

Query: 307 MLKNMDRSRKETEFALAALMEIPSQYKATLELDILGASRGKAIDRIPLPPPRYGPSTQKT 366
           MLKN+DRSRKE EFALAALMEIPSQYKATLEL +LG SRGKAIDRIPLPPP YGPS QKT
Sbjct: 332 MLKNIDRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSIQKT 391

Query: 367 SNSSSSRPIPTSFGGVAPARTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDL 426
           SNSSSSRP P SF GVAP RTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDL
Sbjct: 392 SNSSSSRPTPPSFSGVAPVRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDL 451

Query: 427 QLCPICRSFIDTRIKLY 444
           QLCPICRSFIDTRIKLY
Sbjct: 452 QLCPICRSFIDTRIKLY 466

BLAST of HG10008724 vs. ExPASy TrEMBL
Match: A0A1S4E5G0 (E3 ubiquitin-protein ligase RGLG2-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103502363 PE=4 SV=1)

HSP 1 Score: 828.2 bits (2138), Expect = 1.7e-236
Identity = 414/437 (94.74%), Postives = 420/437 (96.11%), Query Frame = 0

Query: 7   SSCEVMGGKSSKHGSSRRILSSRSNSQQWSQYGYPQSPYDQSRSTPQYRYAPPTPSYGGT 66
           SSCEVMGGKSSKHGSSRR  SSRSNSQQW  YGYP+SPY QSRSTP  RYAPPTPSYGGT
Sbjct: 28  SSCEVMGGKSSKHGSSRRNSSSRSNSQQWGHYGYPESPYTQSRSTP--RYAPPTPSYGGT 87

Query: 67  QAPETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNR 126
           QAPETRKRLERKYSRIDDNYNSL+QVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNR
Sbjct: 88  QAPETRKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNR 147

Query: 127 RSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYPDERFCNG 186
           RSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFY DERFCNG
Sbjct: 148 RSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNG 207

Query: 187 FEEVLGRYRELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTER 246
           FEEVLGRY+ELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTER
Sbjct: 208 FEEVLGRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTER 267

Query: 247 GQFSPQEKKTIEAIVKASEYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNI 306
           GQFSPQEKKTIEAIVKAS+YPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNI
Sbjct: 268 GQFSPQEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNI 327

Query: 307 MLKNMDRSRKETEFALAALMEIPSQYKATLELDILGASRGKAIDRIPLPPPRYGPSTQKT 366
           MLKN+DRSRKE EFALAALMEIPSQYKATLEL +LG SRGKAIDRIPLPPP YGPS QKT
Sbjct: 328 MLKNIDRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSIQKT 387

Query: 367 SNSSSSRPIPTSFGGVAPARTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDL 426
           SNSSSSRP P SF GVAP RTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDL
Sbjct: 388 SNSSSSRPTPPSFSGVAPVRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDL 447

Query: 427 QLCPICRSFIDTRIKLY 444
           QLCPICRSFIDTRIKLY
Sbjct: 448 QLCPICRSFIDTRIKLY 462

BLAST of HG10008724 vs. ExPASy TrEMBL
Match: A0A1S3CLK7 (E3 ubiquitin-protein ligase RGLG2-like isoform X4 OS=Cucumis melo OX=3656 GN=LOC103502363 PE=4 SV=1)

HSP 1 Score: 818.5 bits (2113), Expect = 1.3e-233
Identity = 409/432 (94.68%), Postives = 415/432 (96.06%), Query Frame = 0

Query: 12  MGGKSSKHGSSRRILSSRSNSQQWSQYGYPQSPYDQSRSTPQYRYAPPTPSYGGTQAPET 71
           MGGKSSKHGSSRR  SSRSNSQQW  YGYP+SPY QSRSTP  RYAPPTPSYGGTQAPET
Sbjct: 1   MGGKSSKHGSSRRNSSSRSNSQQWGHYGYPESPYTQSRSTP--RYAPPTPSYGGTQAPET 60

Query: 72  RKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 131
           RKRLERKYSRIDDNYNSL+QVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH
Sbjct: 61  RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 120

Query: 132 IGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYPDERFCNGFEEVL 191
           IGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFY DERFCNGFEEVL
Sbjct: 121 IGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL 180

Query: 192 GRYRELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 251
           GRY+ELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP
Sbjct: 181 GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 240

Query: 252 QEKKTIEAIVKASEYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNM 311
           QEKKTIEAIVKAS+YPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKN+
Sbjct: 241 QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI 300

Query: 312 DRSRKETEFALAALMEIPSQYKATLELDILGASRGKAIDRIPLPPPRYGPSTQKTSNSSS 371
           DRSRKE EFALAALMEIPSQYKATLEL +LG SRGKAIDRIPLPPP YGPS QKTSNSSS
Sbjct: 301 DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSIQKTSNSSS 360

Query: 372 SRPIPTSFGGVAPARTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 431
           SRP P SF GVAP RTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI
Sbjct: 361 SRPTPPSFSGVAPVRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 420

Query: 432 CRSFIDTRIKLY 444
           CRSFIDTRIKLY
Sbjct: 421 CRSFIDTRIKLY 430

BLAST of HG10008724 vs. TAIR 10
Match: AT5G14420.1 (RING domain ligase2 )

HSP 1 Score: 572.8 bits (1475), Expect = 2.4e-163
Identity = 302/458 (65.94%), Postives = 345/458 (75.33%), Query Frame = 0

Query: 15  KSSKHGSSRRILSSRSNSQQWSQYG-----YPQSP----------------YDQSRSTPQ 74
           +S+   S+    SS ++ Q + QYG     YP  P                  Q  S P 
Sbjct: 16  RSTSASSASPSSSSWASQQSYPQYGAESYNYPPPPSYAQPPEYTQPPPPLYSTQPYSAPS 75

Query: 75  YRYAPPTPSYGGTQAPETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFT 134
           Y  APP+ SYG     + +KRLERKYS+I D+Y+SLEQVTEALARAGLESSNLIVGIDFT
Sbjct: 76  YS-APPSQSYGS----DNKKRLERKYSKISDDYSSLEQVTEALARAGLESSNLIVGIDFT 135

Query: 135 KSNEWTGARSFNRRSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQE 194
           KSNEWTGARSFNR+SLH IG   NPYEQAI IIG+TL++FDEDN+IPC+GFGDASTHDQ+
Sbjct: 136 KSNEWTGARSFNRKSLHFIGSSPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQD 195

Query: 195 VFSFYPDERFCNGFEEVLGRYRELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVII 254
           VFSF  ++RFCNGFEEVL RY+E+VP L+LAGPTSFAPII+MA+TIVEQSGGQYHVLVII
Sbjct: 196 VFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQSGGQYHVLVII 255

Query: 255 ADGQVTRSVDTERGQFSPQEKKTIEAIVKASEYPLSIILVGVGDGPWDMMREFDDNIPAR 314
           ADGQVTRSVDTE GQ SPQE+KT++AIV+AS+ PLSI+LVGVGDGPWDMMREFDDNIPAR
Sbjct: 256 ADGQVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWDMMREFDDNIPAR 315

Query: 315 AFDNFQFVNFTNIMLKNMDRSRKETEFALAALMEIPSQYKATLELDILGASRGKAIDRIP 374
           AFDNFQFVNFT IM KN  +S KETEFAL+ALMEIP QYKAT+EL++LG   G   +R P
Sbjct: 316 AFDNFQFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIELNLLGRRNGYIPERFP 375

Query: 375 LPPPRYGPSTQKTSNSSSSRPI--------PTSFGGVAPARTAPPLSSVSDAHACPICIS 434
           LPPP  G S+   S   S  P         PT    V  +   PP SS SD   CPIC+S
Sbjct: 376 LPPPMRGGSSSYNSPKPSRLPSFKPSVPPHPTEGYHVRSSPVPPPTSSASDNQLCPICLS 435

Query: 435 NAKDMAFGCGHQTCCECGQDLQLCPICRSFIDTRIKLY 444
           N KDMAFGCGHQTCCECG DLQ+CPICR+ I TRIKLY
Sbjct: 436 NPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKLY 468

BLAST of HG10008724 vs. TAIR 10
Match: AT5G14420.3 (RING domain ligase2 )

HSP 1 Score: 572.8 bits (1475), Expect = 2.4e-163
Identity = 302/458 (65.94%), Postives = 345/458 (75.33%), Query Frame = 0

Query: 15  KSSKHGSSRRILSSRSNSQQWSQYG-----YPQSP----------------YDQSRSTPQ 74
           +S+   S+    SS ++ Q + QYG     YP  P                  Q  S P 
Sbjct: 16  RSTSASSASPSSSSWASQQSYPQYGAESYNYPPPPSYAQPPEYTQPPPPLYSTQPYSAPS 75

Query: 75  YRYAPPTPSYGGTQAPETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFT 134
           Y  APP+ SYG     + +KRLERKYS+I D+Y+SLEQVTEALARAGLESSNLIVGIDFT
Sbjct: 76  YS-APPSQSYGS----DNKKRLERKYSKISDDYSSLEQVTEALARAGLESSNLIVGIDFT 135

Query: 135 KSNEWTGARSFNRRSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQE 194
           KSNEWTGARSFNR+SLH IG   NPYEQAI IIG+TL++FDEDN+IPC+GFGDASTHDQ+
Sbjct: 136 KSNEWTGARSFNRKSLHFIGSSPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQD 195

Query: 195 VFSFYPDERFCNGFEEVLGRYRELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVII 254
           VFSF  ++RFCNGFEEVL RY+E+VP L+LAGPTSFAPII+MA+TIVEQSGGQYHVLVII
Sbjct: 196 VFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQSGGQYHVLVII 255

Query: 255 ADGQVTRSVDTERGQFSPQEKKTIEAIVKASEYPLSIILVGVGDGPWDMMREFDDNIPAR 314
           ADGQVTRSVDTE GQ SPQE+KT++AIV+AS+ PLSI+LVGVGDGPWDMMREFDDNIPAR
Sbjct: 256 ADGQVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWDMMREFDDNIPAR 315

Query: 315 AFDNFQFVNFTNIMLKNMDRSRKETEFALAALMEIPSQYKATLELDILGASRGKAIDRIP 374
           AFDNFQFVNFT IM KN  +S KETEFAL+ALMEIP QYKAT+EL++LG   G   +R P
Sbjct: 316 AFDNFQFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIELNLLGRRNGYIPERFP 375

Query: 375 LPPPRYGPSTQKTSNSSSSRPI--------PTSFGGVAPARTAPPLSSVSDAHACPICIS 434
           LPPP  G S+   S   S  P         PT    V  +   PP SS SD   CPIC+S
Sbjct: 376 LPPPMRGGSSSYNSPKPSRLPSFKPSVPPHPTEGYHVRSSPVPPPTSSASDNQLCPICLS 435

Query: 435 NAKDMAFGCGHQTCCECGQDLQLCPICRSFIDTRIKLY 444
           N KDMAFGCGHQTCCECG DLQ+CPICR+ I TRIKLY
Sbjct: 436 NPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKLY 468

BLAST of HG10008724 vs. TAIR 10
Match: AT5G14420.4 (RING domain ligase2 )

HSP 1 Score: 572.8 bits (1475), Expect = 2.4e-163
Identity = 302/458 (65.94%), Postives = 345/458 (75.33%), Query Frame = 0

Query: 15  KSSKHGSSRRILSSRSNSQQWSQYG-----YPQSP----------------YDQSRSTPQ 74
           +S+   S+    SS ++ Q + QYG     YP  P                  Q  S P 
Sbjct: 16  RSTSASSASPSSSSWASQQSYPQYGAESYNYPPPPSYAQPPEYTQPPPPLYSTQPYSAPS 75

Query: 75  YRYAPPTPSYGGTQAPETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFT 134
           Y  APP+ SYG     + +KRLERKYS+I D+Y+SLEQVTEALARAGLESSNLIVGIDFT
Sbjct: 76  YS-APPSQSYGS----DNKKRLERKYSKISDDYSSLEQVTEALARAGLESSNLIVGIDFT 135

Query: 135 KSNEWTGARSFNRRSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQE 194
           KSNEWTGARSFNR+SLH IG   NPYEQAI IIG+TL++FDEDN+IPC+GFGDASTHDQ+
Sbjct: 136 KSNEWTGARSFNRKSLHFIGSSPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQD 195

Query: 195 VFSFYPDERFCNGFEEVLGRYRELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVII 254
           VFSF  ++RFCNGFEEVL RY+E+VP L+LAGPTSFAPII+MA+TIVEQSGGQYHVLVII
Sbjct: 196 VFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQSGGQYHVLVII 255

Query: 255 ADGQVTRSVDTERGQFSPQEKKTIEAIVKASEYPLSIILVGVGDGPWDMMREFDDNIPAR 314
           ADGQVTRSVDTE GQ SPQE+KT++AIV+AS+ PLSI+LVGVGDGPWDMMREFDDNIPAR
Sbjct: 256 ADGQVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWDMMREFDDNIPAR 315

Query: 315 AFDNFQFVNFTNIMLKNMDRSRKETEFALAALMEIPSQYKATLELDILGASRGKAIDRIP 374
           AFDNFQFVNFT IM KN  +S KETEFAL+ALMEIP QYKAT+EL++LG   G   +R P
Sbjct: 316 AFDNFQFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIELNLLGRRNGYIPERFP 375

Query: 375 LPPPRYGPSTQKTSNSSSSRPI--------PTSFGGVAPARTAPPLSSVSDAHACPICIS 434
           LPPP  G S+   S   S  P         PT    V  +   PP SS SD   CPIC+S
Sbjct: 376 LPPPMRGGSSSYNSPKPSRLPSFKPSVPPHPTEGYHVRSSPVPPPTSSASDNQLCPICLS 435

Query: 435 NAKDMAFGCGHQTCCECGQDLQLCPICRSFIDTRIKLY 444
           N KDMAFGCGHQTCCECG DLQ+CPICR+ I TRIKLY
Sbjct: 436 NPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKLY 468

BLAST of HG10008724 vs. TAIR 10
Match: AT5G14420.2 (RING domain ligase2 )

HSP 1 Score: 572.8 bits (1475), Expect = 2.4e-163
Identity = 302/458 (65.94%), Postives = 345/458 (75.33%), Query Frame = 0

Query: 15  KSSKHGSSRRILSSRSNSQQWSQYG-----YPQSP----------------YDQSRSTPQ 74
           +S+   S+    SS ++ Q + QYG     YP  P                  Q  S P 
Sbjct: 16  RSTSASSASPSSSSWASQQSYPQYGAESYNYPPPPSYAQPPEYTQPPPPLYSTQPYSAPS 75

Query: 75  YRYAPPTPSYGGTQAPETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFT 134
           Y  APP+ SYG     + +KRLERKYS+I D+Y+SLEQVTEALARAGLESSNLIVGIDFT
Sbjct: 76  YS-APPSQSYGS----DNKKRLERKYSKISDDYSSLEQVTEALARAGLESSNLIVGIDFT 135

Query: 135 KSNEWTGARSFNRRSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQE 194
           KSNEWTGARSFNR+SLH IG   NPYEQAI IIG+TL++FDEDN+IPC+GFGDASTHDQ+
Sbjct: 136 KSNEWTGARSFNRKSLHFIGSSPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQD 195

Query: 195 VFSFYPDERFCNGFEEVLGRYRELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVII 254
           VFSF  ++RFCNGFEEVL RY+E+VP L+LAGPTSFAPII+MA+TIVEQSGGQYHVLVII
Sbjct: 196 VFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQSGGQYHVLVII 255

Query: 255 ADGQVTRSVDTERGQFSPQEKKTIEAIVKASEYPLSIILVGVGDGPWDMMREFDDNIPAR 314
           ADGQVTRSVDTE GQ SPQE+KT++AIV+AS+ PLSI+LVGVGDGPWDMMREFDDNIPAR
Sbjct: 256 ADGQVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWDMMREFDDNIPAR 315

Query: 315 AFDNFQFVNFTNIMLKNMDRSRKETEFALAALMEIPSQYKATLELDILGASRGKAIDRIP 374
           AFDNFQFVNFT IM KN  +S KETEFAL+ALMEIP QYKAT+EL++LG   G   +R P
Sbjct: 316 AFDNFQFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIELNLLGRRNGYIPERFP 375

Query: 375 LPPPRYGPSTQKTSNSSSSRPI--------PTSFGGVAPARTAPPLSSVSDAHACPICIS 434
           LPPP  G S+   S   S  P         PT    V  +   PP SS SD   CPIC+S
Sbjct: 376 LPPPMRGGSSSYNSPKPSRLPSFKPSVPPHPTEGYHVRSSPVPPPTSSASDNQLCPICLS 435

Query: 435 NAKDMAFGCGHQTCCECGQDLQLCPICRSFIDTRIKLY 444
           N KDMAFGCGHQTCCECG DLQ+CPICR+ I TRIKLY
Sbjct: 436 NPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKLY 468

BLAST of HG10008724 vs. TAIR 10
Match: AT3G01650.1 (RING domain ligase1 )

HSP 1 Score: 562.8 bits (1449), Expect = 2.5e-160
Identity = 288/411 (70.07%), Postives = 329/411 (80.05%), Query Frame = 0

Query: 36  SQYG--YPQSPYDQSRSTPQYRYAPPTPSYGGTQAPETRKRLERKYSRIDDNYNSLEQVT 95
           S YG  Y Q  Y  + S P Y   PP PS         RK+ +R+YS+I DNY+SL QV+
Sbjct: 91  SSYGHQYSQEGYASAASQPNY---PPPPSQSQV---ADRKKFDRRYSKISDNYSSLLQVS 150

Query: 96  EALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIGDEQNPYEQAIAIIGQTLSSF 155
           EAL RAGLESSNLIVGIDFTKSNEWTGA+SFNR+SLHH+ +  NPYEQAI IIG+TL++F
Sbjct: 151 EALGRAGLESSNLIVGIDFTKSNEWTGAKSFNRKSLHHLSNTPNPYEQAITIIGRTLAAF 210

Query: 156 DEDNMIPCFGFGDASTHDQEVFSFYPDERFCNGFEEVLGRYRELVPHLRLAGPTSFAPII 215
           DEDN+IPC+GFGDASTHDQ+VFSFYP+ RFCNGFEEVL RYRE+VP L+LAGPTSFAPII
Sbjct: 211 DEDNLIPCYGFGDASTHDQDVFSFYPEGRFCNGFEEVLARYREIVPQLKLAGPTSFAPII 270

Query: 216 EMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSPQEKKTIEAIVKASEYPLSIILV 275
           EMA+T+VEQS GQYHVLVIIADGQVTRSVDTE G+ SPQE+KT++AIVKAS  PLSI+LV
Sbjct: 271 EMAMTVVEQSSGQYHVLVIIADGQVTRSVDTEHGRLSPQEQKTVDAIVKASTLPLSIVLV 330

Query: 276 GVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNMDRSRKETEFALAALMEIPSQYK 335
           GVGDGPWDMM+EFDDNIPARAFDNFQFVNFT IM KN D+SRKETEFAL+ALMEIP QYK
Sbjct: 331 GVGDGPWDMMQEFDDNIPARAFDNFQFVNFTEIMSKNKDQSRKETEFALSALMEIPPQYK 390

Query: 336 ATLELDILGASRGKAIDRIPLPPPRYGPSTQKTSNSSSSRPIPTSFGGVAP-ARTAPPLS 395
           AT+EL++LG   G    RIPLPPP      Q  S+ SSSR IP     V P    +  +S
Sbjct: 391 ATIELNLLGVRNGNIPQRIPLPPP-----VQSGSSFSSSR-IPNFEPSVPPYPFESKQMS 450

Query: 396 SVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIDTRIKLY 444
           S  D   CPIC+SN K+MAFGCGHQTCCECG DL++CPICR+ I TRIKLY
Sbjct: 451 SADDIQLCPICLSNPKNMAFGCGHQTCCECGPDLKVCPICRAPIQTRIKLY 489