HG10008693 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10008693
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionjmjC domain-containing protein 4 isoform X1
LocationChr10: 25315759 .. 25320045 (-)
RNA-Seq ExpressionHG10008693
SyntenyHG10008693
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTATCGAAATTTGTGGAAGAATAGACAAAGTAAATGGAAAAGAACTGAGTTATAAGGAATTTGTAGAACGATACATGGAGAAGAACCAACCTGTGGTGTTGACAGGTCTCATGGATGATTGGAAGGCTTGCAGCGATTGGGTCGACGAGAATGGGGAGGCAAATCTAGGGTTCTTTTCCACCCATTTCGGGAAATCCAGGGTACAGGTACTTAAATTTCTGCTAGAAGATTATCTTTTTGGTTCTGATTGTGCGTGCTCAATTTTCATATATACTCTTTTATGTCTCATCTCATTTCAGCATGATGCATTGTGATTCAAATTTTTTGTATATCCGGGGATTTATTCAGTGGAATAAGTGGGTTGCTTTTATTCTTGCATCTTTTATTTATCATTTCCTTGTTTTAAGAAACAGCATGAAGAAGGATGAAAGTTTCCTCGTTGCTTAAAGATTAATGATCCAGTAGAATTATCCCCTTATGTTCTGTTTTGGAGGTTGGTAAATGATAAAAATGAGAAAGAAGACAGAAAATGAATTGATCCATTTTATTGCTATTAGTTAACAAGGAATGATGCAAAAACGATCAGCCCTGTTTGCCTGATTAACCATACATTTTTTGCCCCTTATTGCAATATGACTCTTGGGGGAAAATATTGGGAATGCCGTTCTTCCAGTCTCCCTCATTTGCAATGTCTTACCATAGAAAGCAAAGGAAGAGGGGAGCACTCATTGAATTTCGTAATATGTGATTGGGCCATCTCATTTAGTTGATGTGTCCATGCTTAAATGTACCATTAATGGGAATACTTCTCCTATCATGAAACTTGTCCATTCATGTTCAACTACCCACTTGAATGTTTTCAAAAAAAAGTAAAAGGAAGAAAGAAGAAGAAAAGAAAGAGTTTTAAGTCTGTATTCCACTTTTTATGAGGTCAGATAATCATTTGCCACTTGTAGGTTGCAGATTGCAGTACTAGAGAATTTACAGATCAGAAGAGAGTAGAAATGTCTGTTTCAGAGTTCATTGATCAATGGTGTAAGGAGCCTATACAGGAGCATGGCCTTACCTCAAACAACGGACTTACAGACAAGTCTGTACTATACTTGAAGGACTGGCATTTTGTGAAGGTGTAAGCGTCTACTTATTATTCCTTTTTTATTATACTTACTGAGTTAGAACTAATGGATATATATGTCTATAAGGAACAACAAATTGTAGGCCTTAAGAAAGTTCATTATTTTACTGCTTCTAGTGTCAAATTACTAGGTAAAATGCTTGGTTGGAACACTTGTGCAAGACGAACATGGCTCTTTTAACCCCACGTGATCAAGCACGTTTTATTAACAAGTGCATTTTTTAGGATAGAATTATCATTTTTGTTGACGAGTGGATTGGAATGCAGTTCATCTCATGTAGCTTAACTTTGAATCTCAACCATGGAAACGCTTAGGAACATCTTTCTTTTTCAACTGTGGATAACAATTAAAGGGTATATAGGGAATGGTGAAAACGTCTTGTATGGTTTCTCTACATATCCTATGAATAGTCCTATGAATAGAATTTTGTATTCAACATTCATTGTACTGGAACTGTGAGTATCCTGCTTAACTTCTCCTTGACTAATCCCACATGTATGTGCACACACACATGCACAGATTATTGACTATTTGAGTCATTATCTTAAATTTCCATGCTGGATTGAATTTTACATTATCATCTTACATTATTATTGTTTGGAACATGCTGCAATAGATTTTTGGTTGCATGACTGTCAAATTATGGGCTTTGTTAGTAAGAGAATAGCTAGATATGTGGTCTCACATGTTTTCCTATCCTTGTTGATCTGATTCTCAGGAATATCCAAATTATACAGCATACTCAACTCCACACTTTGTTTGTGATGACTGGCTCAACCTATACCTTGACAGTTACCGTATGCACAGGGATCCTGACTCTTACCAAGAGAAAGATGAAATATGTTGCTCTGACTATCGTTTCGTTTACATGGGAGCAAAAGGTGTAGCATCCTATAAAACTGTTGCACTTTTTTTAATGTTTGGCCTCATCAAAACTTATACATGGTTCTTAATCAGGTTCTTGGACTCCACTTCATGCCGACGTATTCAGGTCATACAGCTGGTCAGCTAATGTTTGCGGAAAGAAACAATGGTTTCTTTTACCTCCTTCTCAGTCTCATCTTGTATTTGACAGGCATGGACATCGTTTTGTTTTTTTGCACTTTCCTGTTAACTGATGGTTCAATGTGTTGGATGTTGTTCTTGTTCTAATTTTGTAAGTAGCCACCAATTTCTGTTCTGAAGGGACTCGTGGACTTGATTGACTAATTCTTTCCATTACATTAGTACTTGAGATTGAGAAGAGAAAAGTGATCGAAAAGATGCTAAAACTCTAAGCTGAAAAGAACCTATGAGTTGTCCATATTTTCTATTTCCAAGCTTTGGTATGTAAATGTGACAACAAACAATCATGATCTTTCTCACATCATATATTTAGAGTCATTCGATATCAACCCCCCCCCCCCCCCCCCCCTTCTCTCTTTAAATGCACGTCTTTGAGCTTTGTCATGGTTTCCTCTTTTTCTAACCTTTTGTTTATATTTTCAAACAGGAATATGAAGGCCTGTATATATAGTATCTTTGATGATATAAGTGAAAATTTGTTTCCTGGTTTTAAGAAGGTAAATATGATCCAACCTCTTCTCCTTCTATCTCTTGAATATATCAGCTTTTGCATGCTTTTTTGTTTATCATTATGCAGGCTACCTGGTTGGATTGTACTCAAGAACAGAATGAAATTATATTTGTGCCAAGTGGCTGGTATCATCAAGTTCATAATCTGGTATTACACTTCCTTTATTATGTCCTTGTGGTGCAAATTAATTTGTATATTTCATGGTTTCAGGGATCATTATTTTATGATAACTTGTACCTGTTCCACTATGATAGATATCTTTAATTGTCCTTTACATTCTCAGCCTCCATTCTATTATTTGATAGATATCATTATTTTCTTGACGGATGCTTGATTCAGTTTGCCTTTTCTTTTTCTGAAGATATTTATTTAACAGTTGAACTTTGAGGTTCATCCATATTTTTCATCATAGTCTGTCCGCCCAACAACCGTTCTATCTTTTGATATGTGTTTGTATCTTTTCAGGATGACACTGTATCAATAAACCACAATTGGTTTAACTCCTACAACCTCTGTTGGGTGGTAAGGATTGCTATCATGGGCATTACATAATCTTTCTTCACAACAGGATAAACAATATTCTGATCCAACATTCTTTTAGTTGGACTTGATGTTGCGGGACTACAACGAAGCTAAGGAGTACATAGAAGACATTAGAGATATCTGTGATGATTTTGAAGGTCTCTGTCAGCGCAACCTCGCGGCTAACACAGGTAGCATAAAGTTTTGGATGACTAAGTATAGCACCTTAGGGTGTGGTTGAATCTTTCTGTAAAATTTGACATTGTAACATTCTGACCAGATATACAATTTGCAGCGAGGAAACTACCCGAACAACTAGATCGTCTTCAATAATTTGAGTCGTGAATACATTATCTCATTATCTAGTTTAATTTAGAGCTGGACATTTACTTTCAAATCTTTACTCAGCTTGTCGAGATCAGAGTGTGACTAGGGAGCAAGCTCTTAGAAATATTTTCTCATTATTAGGAGTTTTCGGTGGAAAGCTCTCTAAGATGCATATACTGCTAGTATTAAGGAAGGAATGTGCATGCGCACTACACCAATGATATGGTAGTACTAGTTAATTTATACAATTTTTTTCTCCCCTTTCAGGCATGAACTTCTATGATTTCTTCATCTTTTTAGCACGCTTTTCGCTCGCCAATTTGGTGGTACTTGACACTCTAGCAAGAGACGCTGAAAATGTTGCTGGGAGCTTATCTCCAATGGTTCAACAGTTGGCTCAGAATCTTGCTTCAATACAGAGGATTGCCTTGAAAATGAAATTCTTGGAATGTTTTTCAGAAGACCAAGGATTCATGTTGGATCTAGGAGAAACACACAAAGATCCCAAGTTTTCTAAACTCTGTTACTCTTTAATTCAAACTTGTGGGTCCATACACAAACAACAGATTTCGAGCTTTTGCCCAAAGACAATCGCAACACATGATTCTAGGACTTCAATTATCATTCAAAATTTTTGTGATGGTGTTTCGTCGCCCAAAGATCTGGTCAAATTTATCGACACGGTCTTAACCAAACATACAAACTTAGAATCACCAGAATGA

mRNA sequence

ATGGGTATCGAAATTTGTGGAAGAATAGACAAAGTAAATGGAAAAGAACTGAGTTATAAGGAATTTGTAGAACGATACATGGAGAAGAACCAACCTGTGGTGTTGACAGGTCTCATGGATGATTGGAAGGCTTGCAGCGATTGGGTCGACGAGAATGGGGAGGCAAATCTAGGGTTCTTTTCCACCCATTTCGGGAAATCCAGGGTACAGGTTGCAGATTGCAGTACTAGAGAATTTACAGATCAGAAGAGAGTAGAAATGTCTGTTTCAGAGTTCATTGATCAATGGTGTAAGGAGCCTATACAGGAGCATGGCCTTACCTCAAACAACGGACTTACAGACAAGTCTGTACTATACTTGAAGGACTGGCATTTTGTGAAGGAATATCCAAATTATACAGCATACTCAACTCCACACTTTGTTTGTGATGACTGGCTCAACCTATACCTTGACAGTTACCGTATGCACAGGGATCCTGACTCTTACCAAGAGAAAGATGAAATATGTTGCTCTGACTATCGTTTCGTTTACATGGGAGCAAAAGGTTCTTGGACTCCACTTCATGCCGACGTATTCAGGTCATACAGCTGGTCAGCTAATGTTTGCGGAAAGAAACAATGGAATATGAAGGCCTGTATATATAGTATCTTTGATGATATAAGTGAAAATTTGTTTCCTGGTTTTAAGAAGGCTACCTGGTTGGATTGTACTCAAGAACAGAATGAAATTATATTTGTGCCAAGTGGCTGGTATCATCAAGTTCATAATCTGGATGACACTGTATCAATAAACCACAATTGGTTTAACTCCTACAACCTCTGTTGGGTGTTGGACTTGATGTTGCGGGACTACAACGAAGCTAAGGAGTACATAGAAGACATTAGAGATATCTGTGATGATTTTGAAGGTCTCTGTCAGCGCAACCTCGCGGCTAACACAGGCATGAACTTCTATGATTTCTTCATCTTTTTAGCACGCTTTTCGCTCGCCAATTTGGTGGTACTTGACACTCTAGCAAGAGACGCTGAAAATGTTGCTGGGAGCTTATCTCCAATGGTTCAACAGTTGGCTCAGAATCTTGCTTCAATACAGAGGATTGCCTTGAAAATGAAATTCTTGGAATGTTTTTCAGAAGACCAAGGATTCATGTTGGATCTAGGAGAAACACACAAAGATCCCAAGTTTTCTAAACTCTGTTACTCTTTAATTCAAACTTGTGGGTCCATACACAAACAACAGATTTCGAGCTTTTGCCCAAAGACAATCGCAACACATGATTCTAGGACTTCAATTATCATTCAAAATTTTTGTGATGGTGTTTCGTCGCCCAAAGATCTGGTCAAATTTATCGACACGGTCTTAACCAAACATACAAACTTAGAATCACCAGAATGA

Coding sequence (CDS)

ATGGGTATCGAAATTTGTGGAAGAATAGACAAAGTAAATGGAAAAGAACTGAGTTATAAGGAATTTGTAGAACGATACATGGAGAAGAACCAACCTGTGGTGTTGACAGGTCTCATGGATGATTGGAAGGCTTGCAGCGATTGGGTCGACGAGAATGGGGAGGCAAATCTAGGGTTCTTTTCCACCCATTTCGGGAAATCCAGGGTACAGGTTGCAGATTGCAGTACTAGAGAATTTACAGATCAGAAGAGAGTAGAAATGTCTGTTTCAGAGTTCATTGATCAATGGTGTAAGGAGCCTATACAGGAGCATGGCCTTACCTCAAACAACGGACTTACAGACAAGTCTGTACTATACTTGAAGGACTGGCATTTTGTGAAGGAATATCCAAATTATACAGCATACTCAACTCCACACTTTGTTTGTGATGACTGGCTCAACCTATACCTTGACAGTTACCGTATGCACAGGGATCCTGACTCTTACCAAGAGAAAGATGAAATATGTTGCTCTGACTATCGTTTCGTTTACATGGGAGCAAAAGGTTCTTGGACTCCACTTCATGCCGACGTATTCAGGTCATACAGCTGGTCAGCTAATGTTTGCGGAAAGAAACAATGGAATATGAAGGCCTGTATATATAGTATCTTTGATGATATAAGTGAAAATTTGTTTCCTGGTTTTAAGAAGGCTACCTGGTTGGATTGTACTCAAGAACAGAATGAAATTATATTTGTGCCAAGTGGCTGGTATCATCAAGTTCATAATCTGGATGACACTGTATCAATAAACCACAATTGGTTTAACTCCTACAACCTCTGTTGGGTGTTGGACTTGATGTTGCGGGACTACAACGAAGCTAAGGAGTACATAGAAGACATTAGAGATATCTGTGATGATTTTGAAGGTCTCTGTCAGCGCAACCTCGCGGCTAACACAGGCATGAACTTCTATGATTTCTTCATCTTTTTAGCACGCTTTTCGCTCGCCAATTTGGTGGTACTTGACACTCTAGCAAGAGACGCTGAAAATGTTGCTGGGAGCTTATCTCCAATGGTTCAACAGTTGGCTCAGAATCTTGCTTCAATACAGAGGATTGCCTTGAAAATGAAATTCTTGGAATGTTTTTCAGAAGACCAAGGATTCATGTTGGATCTAGGAGAAACACACAAAGATCCCAAGTTTTCTAAACTCTGTTACTCTTTAATTCAAACTTGTGGGTCCATACACAAACAACAGATTTCGAGCTTTTGCCCAAAGACAATCGCAACACATGATTCTAGGACTTCAATTATCATTCAAAATTTTTGTGATGGTGTTTCGTCGCCCAAAGATCTGGTCAAATTTATCGACACGGTCTTAACCAAACATACAAACTTAGAATCACCAGAATGA

Protein sequence

MGIEICGRIDKVNGKELSYKEFVERYMEKNQPVVLTGLMDDWKACSDWVDENGEANLGFFSTHFGKSRVQVADCSTREFTDQKRVEMSVSEFIDQWCKEPIQEHGLTSNNGLTDKSVLYLKDWHFVKEYPNYTAYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEICCSDYRFVYMGAKGSWTPLHADVFRSYSWSANVCGKKQWNMKACIYSIFDDISENLFPGFKKATWLDCTQEQNEIIFVPSGWYHQVHNLDDTVSINHNWFNSYNLCWVLDLMLRDYNEAKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARFSLANLVVLDTLARDAENVAGSLSPMVQQLAQNLASIQRIALKMKFLECFSEDQGFMLDLGETHKDPKFSKLCYSLIQTCGSIHKQQISSFCPKTIATHDSRTSIIIQNFCDGVSSPKDLVKFIDTVLTKHTNLESPE
Homology
BLAST of HG10008693 vs. NCBI nr
Match: XP_038879491.1 (2-oxoglutarate and iron-dependent oxygenase JMJD4 isoform X2 [Benincasa hispida])

HSP 1 Score: 918.7 bits (2373), Expect = 2.0e-263
Identity = 436/478 (91.21%), Postives = 448/478 (93.72%), Query Frame = 0

Query: 1   MGIEICGRIDKVNGKELSYKEFVERYMEKNQPVVLTGLMDDWKACSDWVDENGEANLGFF 60
           MGIEICGRIDKVNGK LSYKEFVERYMEKNQPVVLTGLMDDWKACSDWVDEN + NLGFF
Sbjct: 1   MGIEICGRIDKVNGKGLSYKEFVERYMEKNQPVVLTGLMDDWKACSDWVDENRQPNLGFF 60

Query: 61  STHFGKSRVQVADCSTREFTDQKRVEMSVSEFIDQWCKEPIQEHGLTSNNGLTDKSVLYL 120
           STHFGKSRVQVADCSTREFTDQKRVEMSVSEFIDQWCKEPIQEHGL S++GLT+KSVLYL
Sbjct: 61  STHFGKSRVQVADCSTREFTDQKRVEMSVSEFIDQWCKEPIQEHGLASDHGLTNKSVLYL 120

Query: 121 KDWHFVKEYPNYTAYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEICCSDYRFVYMGA 180
           KDWHFVKEYPNYTAYSTPHFVCDDWLNLYLDSYRMHRD DSYQEKDEI CSDYRFVYMGA
Sbjct: 121 KDWHFVKEYPNYTAYSTPHFVCDDWLNLYLDSYRMHRDSDSYQEKDEISCSDYRFVYMGA 180

Query: 181 KGSWTPLHADVFRSYSWSANVCGKKQW--------------NMKACIYSIFDDISENLFP 240
           KGSWTPLHADVFRSYSWSANVCGKKQW              NMKACIY+IFDDISE+LFP
Sbjct: 181 KGSWTPLHADVFRSYSWSANVCGKKQWFLLPPSQSHLVFDRNMKACIYNIFDDISEDLFP 240

Query: 241 GFKKATWLDCTQEQNEIIFVPSGWYHQVHNLDDTVSINHNWFNSYNLCWVLDLMLRDYNE 300
           GFKKA WL+CTQEQNEIIFVPSGWYHQVHNLDDTVSINHNWFNSYNLCWVLDLM RDYNE
Sbjct: 241 GFKKAAWLECTQEQNEIIFVPSGWYHQVHNLDDTVSINHNWFNSYNLCWVLDLMSRDYNE 300

Query: 301 AKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARFSLANLVVLDTLARDAENVA 360
           AKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLA FSLANLVVLDTLARD ENVA
Sbjct: 301 AKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLACFSLANLVVLDTLARDTENVA 360

Query: 361 GSLSPMVQQLAQNLASIQRIALKMKFLECFSEDQGFMLDLGETHKDPKFSKLCYSLIQTC 420
           GS SPMVQQLAQNLASIQ+IALKMKFLECFSEDQGF+LDL ETHKDP FSKLCYSLIQTC
Sbjct: 361 GSSSPMVQQLAQNLASIQKIALKMKFLECFSEDQGFVLDLEETHKDPNFSKLCYSLIQTC 420

Query: 421 GSIHKQQISSFCPKTIATHDSRTSIIIQNFCDGVSSPKDLVKFIDTVLTKHTNLESPE 465
           GSIHKQQIS+FC K IATHDSRTS +IQNFCDGVSSP+DLVKFIDTVLTKHTNLESPE
Sbjct: 421 GSIHKQQISNFCTKAIATHDSRTSNVIQNFCDGVSSPEDLVKFIDTVLTKHTNLESPE 478

BLAST of HG10008693 vs. NCBI nr
Match: XP_038879489.1 (2-oxoglutarate and iron-dependent oxygenase JMJD4 isoform X1 [Benincasa hispida] >XP_038879490.1 2-oxoglutarate and iron-dependent oxygenase JMJD4 isoform X1 [Benincasa hispida])

HSP 1 Score: 902.9 bits (2332), Expect = 1.1e-258
Identity = 436/508 (85.83%), Postives = 448/508 (88.19%), Query Frame = 0

Query: 1   MGIEICGRIDKVNGKELSYKEFVERYMEKNQPVVLTGLMDDWKACSDWVDENGEANLGFF 60
           MGIEICGRIDKVNGK LSYKEFVERYMEKNQPVVLTGLMDDWKACSDWVDEN + NLGFF
Sbjct: 1   MGIEICGRIDKVNGKGLSYKEFVERYMEKNQPVVLTGLMDDWKACSDWVDENRQPNLGFF 60

Query: 61  STHFGKSRVQVADCSTREFTDQKRVEMSVSEFIDQWCKEPIQEHGLTSNNGLTDKSVLYL 120
           STHFGKSRVQVADCSTREFTDQKRVEMSVSEFIDQWCKEPIQEHGL S++GLT+KSVLYL
Sbjct: 61  STHFGKSRVQVADCSTREFTDQKRVEMSVSEFIDQWCKEPIQEHGLASDHGLTNKSVLYL 120

Query: 121 KDWHFVKEYPNYTAYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEICCSDYRFVYMGA 180
           KDWHFVKEYPNYTAYSTPHFVCDDWLNLYLDSYRMHRD DSYQEKDEI CSDYRFVYMGA
Sbjct: 121 KDWHFVKEYPNYTAYSTPHFVCDDWLNLYLDSYRMHRDSDSYQEKDEISCSDYRFVYMGA 180

Query: 181 KGSWTPLHADVFRSYSWSANVCGKKQW--------------NMKACIYSIFDDISENLFP 240
           KGSWTPLHADVFRSYSWSANVCGKKQW              NMKACIY+IFDDISE+LFP
Sbjct: 181 KGSWTPLHADVFRSYSWSANVCGKKQWFLLPPSQSHLVFDRNMKACIYNIFDDISEDLFP 240

Query: 241 GFKK------------------------------ATWLDCTQEQNEIIFVPSGWYHQVHN 300
           GFKK                              A WL+CTQEQNEIIFVPSGWYHQVHN
Sbjct: 241 GFKKVSLIPYVQPLLLLSLEYIIFFHVHLFIIMQAAWLECTQEQNEIIFVPSGWYHQVHN 300

Query: 301 LDDTVSINHNWFNSYNLCWVLDLMLRDYNEAKEYIEDIRDICDDFEGLCQRNLAANTGMN 360
           LDDTVSINHNWFNSYNLCWVLDLM RDYNEAKEYIEDIRDICDDFEGLCQRNLAANTGMN
Sbjct: 301 LDDTVSINHNWFNSYNLCWVLDLMSRDYNEAKEYIEDIRDICDDFEGLCQRNLAANTGMN 360

Query: 361 FYDFFIFLARFSLANLVVLDTLARDAENVAGSLSPMVQQLAQNLASIQRIALKMKFLECF 420
           FYDFFIFLA FSLANLVVLDTLARD ENVAGS SPMVQQLAQNLASIQ+IALKMKFLECF
Sbjct: 361 FYDFFIFLACFSLANLVVLDTLARDTENVAGSSSPMVQQLAQNLASIQKIALKMKFLECF 420

Query: 421 SEDQGFMLDLGETHKDPKFSKLCYSLIQTCGSIHKQQISSFCPKTIATHDSRTSIIIQNF 465
           SEDQGF+LDL ETHKDP FSKLCYSLIQTCGSIHKQQIS+FC K IATHDSRTS +IQNF
Sbjct: 421 SEDQGFVLDLEETHKDPNFSKLCYSLIQTCGSIHKQQISNFCTKAIATHDSRTSNVIQNF 480

BLAST of HG10008693 vs. NCBI nr
Match: XP_004138273.2 (2-oxoglutarate and iron-dependent oxygenase JMJD4 isoform X2 [Cucumis sativus] >KGN63564.2 hypothetical protein Csa_013850 [Cucumis sativus])

HSP 1 Score: 894.8 bits (2311), Expect = 3.1e-256
Identity = 422/480 (87.92%), Postives = 446/480 (92.92%), Query Frame = 0

Query: 1   MGIEICGRIDKVNGKELSYKEFVERYMEKNQPVVLTGLMDDWKACSDWVDENGEANLGFF 60
           MGIEICG+IDKVNGK LSYKEFVERYMEKN+PVVLTGLMDDWKACSDWVDENG+ NL FF
Sbjct: 2   MGIEICGKIDKVNGKGLSYKEFVERYMEKNKPVVLTGLMDDWKACSDWVDENGQPNLSFF 61

Query: 61  STHFGKSRVQVADCSTREFTDQKRVEMSVSEFIDQWCKEPIQEHGLTSNNGLTDKSVLYL 120
           STHFGKSRVQVADCST+EFTDQKRVEMSVSEFIDQWCKEPIQEHGL+SNNGLTDKSVLYL
Sbjct: 62  STHFGKSRVQVADCSTKEFTDQKRVEMSVSEFIDQWCKEPIQEHGLSSNNGLTDKSVLYL 121

Query: 121 KDWHFVKEYPNYTAYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEICCSDYRFVYMGA 180
           KDWHFVKEY NYTAYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEI CSDYRFVYMGA
Sbjct: 122 KDWHFVKEYLNYTAYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEISCSDYRFVYMGA 181

Query: 181 KGSWTPLHADVFRSYSWSANVCGKKQW--------------NMKACIYSIFDDISENLFP 240
           KGSWTPLHADVFRSYSWSANVCGKKQW              NMKACIY+IFDDISE LFP
Sbjct: 182 KGSWTPLHADVFRSYSWSANVCGKKQWFLLPPSQSHLVFDRNMKACIYNIFDDISEYLFP 241

Query: 241 GFKKATWLDCTQEQNEIIFVPSGWYHQVHNLDDTVSINHNWFNSYNLCWVLDLMLRDYNE 300
           GFKKATWL+C QEQNEIIFVPSGWYHQVHNLDDTVSINHNWFNSYNLCWVLDLMLRDYNE
Sbjct: 242 GFKKATWLECIQEQNEIIFVPSGWYHQVHNLDDTVSINHNWFNSYNLCWVLDLMLRDYNE 301

Query: 301 AKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARFSLANLVVLDTLARDAENVA 360
           AKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARFSLAN VVLDTLARDAEN+A
Sbjct: 302 AKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARFSLANSVVLDTLARDAENIA 361

Query: 361 --GSLSPMVQQLAQNLASIQRIALKMKFLECFSEDQGFMLDLGETHKDPKFSKLCYSLIQ 420
             GS+SP++QQL QNL+SIQ+IALKMKFLECFS DQGFMLDL ETHK+PKFSKLCYSLI+
Sbjct: 362 GIGSISPIIQQLVQNLSSIQKIALKMKFLECFSGDQGFMLDLVETHKNPKFSKLCYSLIR 421

Query: 421 TCGSIHKQQISSFCPKTIATHDSRTSIIIQNFCDGVSSPKDLVKFIDTVLTKHTNLESPE 465
           TC SIH +Q+ SFC KT+AT DSRTSI+IQNFCD VSSP+DLVK+IDTVLT+HT++ES E
Sbjct: 422 TCESIHNRQVLSFCSKTVATQDSRTSIVIQNFCDAVSSPQDLVKYIDTVLTEHTSIESSE 481

BLAST of HG10008693 vs. NCBI nr
Match: XP_031740132.1 (2-oxoglutarate and iron-dependent oxygenase JMJD4 isoform X1 [Cucumis sativus])

HSP 1 Score: 880.2 bits (2273), Expect = 8.0e-252
Identity = 420/492 (85.37%), Postives = 446/492 (90.65%), Query Frame = 0

Query: 1   MGIEICGRIDKVNGKELSYKEFVERYMEKNQPVVLTGLMDDWKACSDWVDENGEANLGFF 60
           MGIEICG+IDKVNGK LSYKEFVERYMEKN+PVVLTGLMDDWKACSDWVDENG+ NL FF
Sbjct: 2   MGIEICGKIDKVNGKGLSYKEFVERYMEKNKPVVLTGLMDDWKACSDWVDENGQPNLSFF 61

Query: 61  STHFGKSRVQ-----VAD-------CSTREFTDQKRVEMSVSEFIDQWCKEPIQEHGLTS 120
           STHFGKSRVQ     ++D       CST+EFTDQKRVEMSVSEFIDQWCKEPIQEHGL+S
Sbjct: 62  STHFGKSRVQSPSFAISDSYHKKQNCSTKEFTDQKRVEMSVSEFIDQWCKEPIQEHGLSS 121

Query: 121 NNGLTDKSVLYLKDWHFVKEYPNYTAYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEI 180
           NNGLTDKSVLYLKDWHFVKEY NYTAYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEI
Sbjct: 122 NNGLTDKSVLYLKDWHFVKEYLNYTAYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEI 181

Query: 181 CCSDYRFVYMGAKGSWTPLHADVFRSYSWSANVCGKKQW--------------NMKACIY 240
            CSDYRFVYMGAKGSWTPLHADVFRSYSWSANVCGKKQW              NMKACIY
Sbjct: 182 SCSDYRFVYMGAKGSWTPLHADVFRSYSWSANVCGKKQWFLLPPSQSHLVFDRNMKACIY 241

Query: 241 SIFDDISENLFPGFKKATWLDCTQEQNEIIFVPSGWYHQVHNLDDTVSINHNWFNSYNLC 300
           +IFDDISE LFPGFKKATWL+C QEQNEIIFVPSGWYHQVHNLDDTVSINHNWFNSYNLC
Sbjct: 242 NIFDDISEYLFPGFKKATWLECIQEQNEIIFVPSGWYHQVHNLDDTVSINHNWFNSYNLC 301

Query: 301 WVLDLMLRDYNEAKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARFSLANLVV 360
           WVLDLMLRDYNEAKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARFSLAN VV
Sbjct: 302 WVLDLMLRDYNEAKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARFSLANSVV 361

Query: 361 LDTLARDAENVA--GSLSPMVQQLAQNLASIQRIALKMKFLECFSEDQGFMLDLGETHKD 420
           LDTLARDAEN+A  GS+SP++QQL QNL+SIQ+IALKMKFLECFS DQGFMLDL ETHK+
Sbjct: 362 LDTLARDAENIAGIGSISPIIQQLVQNLSSIQKIALKMKFLECFSGDQGFMLDLVETHKN 421

Query: 421 PKFSKLCYSLIQTCGSIHKQQISSFCPKTIATHDSRTSIIIQNFCDGVSSPKDLVKFIDT 465
           PKFSKLCYSLI+TC SIH +Q+ SFC KT+AT DSRTSI+IQNFCD VSSP+DLVK+IDT
Sbjct: 422 PKFSKLCYSLIRTCESIHNRQVLSFCSKTVATQDSRTSIVIQNFCDAVSSPQDLVKYIDT 481

BLAST of HG10008693 vs. NCBI nr
Match: XP_023516598.1 (jmjC domain-containing protein 4 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 874.0 bits (2257), Expect = 5.7e-250
Identity = 415/479 (86.64%), Postives = 432/479 (90.19%), Query Frame = 0

Query: 1   MGIEICGRIDKVNGKELSYKEFVERYMEKNQPVVLTGLMDDWKACSDWVDENGEANLGFF 60
           M IE  GRIDKVNG ELSY+EFVERYMEKNQPVVLTGLMDDWKACSDWVDENG+ NL FF
Sbjct: 2   MAIETSGRIDKVNGNELSYEEFVERYMEKNQPVVLTGLMDDWKACSDWVDENGQPNLRFF 61

Query: 61  STHFGKSRVQVADCSTREFTDQKRVEMSVSEFIDQWCKEPIQEHGLTSNNGLTDKSVLYL 120
           STHFGKS+VQVADC+TREFTDQKRVEMSVSEFIDQWCKE +QE G+TSNNG  DKSVLYL
Sbjct: 62  STHFGKSKVQVADCNTREFTDQKRVEMSVSEFIDQWCKEAVQERGVTSNNGDIDKSVLYL 121

Query: 121 KDWHFVKEYPNYTAYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEICCSDYRFVYMGA 180
           KDWHFVKEYPNY AYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEI CSDYRFVYMGA
Sbjct: 122 KDWHFVKEYPNYAAYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEISCSDYRFVYMGA 181

Query: 181 KGSWTPLHADVFRSYSWSANVCGKKQW--------------NMKACIYSIFDD-ISENLF 240
           KGSWTPLHADVFRSYSWSANVCGKKQW              NMKACIY+IFDD ISENLF
Sbjct: 182 KGSWTPLHADVFRSYSWSANVCGKKQWFLLPPSQSHLVFDRNMKACIYNIFDDNISENLF 241

Query: 241 PGFKKATWLDCTQEQNEIIFVPSGWYHQVHNLDDTVSINHNWFNSYNLCWVLDLMLRDYN 300
           PGFKKATWL+CTQEQNEIIFVPSGWYHQVHNL+DTVSINHNWFNSYNLCWVLDLM RDYN
Sbjct: 242 PGFKKATWLECTQEQNEIIFVPSGWYHQVHNLEDTVSINHNWFNSYNLCWVLDLMARDYN 301

Query: 301 EAKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARFSLANLVVLDTLARDAENV 360
           EAKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARF+LANLVVLD LARD ENV
Sbjct: 302 EAKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARFALANLVVLDNLARDTENV 361

Query: 361 AGSLSPMVQQLAQNLASIQRIALKMKFLECFSEDQGFMLDLGETHKDPKFSKLCYSLIQT 420
           AGSLSPMVQQLA NLASI+ I LKMK LECFS DQGF+LDLGETHKDPKFS LCYSL+QT
Sbjct: 362 AGSLSPMVQQLAHNLASIKNIVLKMKSLECFSGDQGFVLDLGETHKDPKFSNLCYSLVQT 421

Query: 421 CGSIHKQQISSFCPKTIATHDSRTSIIIQNFCDGVSSPKDLVKFIDTVLTKHTNLESPE 465
           CGSIHKQQIS  C KTI THDSR+S IIQNFCDGVSSP+DLV FIDTV T+    E+PE
Sbjct: 422 CGSIHKQQISRLCTKTIDTHDSRSSTIIQNFCDGVSSPQDLVTFIDTVSTERAKSEAPE 480

BLAST of HG10008693 vs. ExPASy Swiss-Prot
Match: Q08BY5 (2-oxoglutarate and iron-dependent oxygenase JMJD4 OS=Danio rerio OX=7955 GN=jmjd4 PE=2 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.8e-60
Identity = 131/371 (35.31%), Postives = 199/371 (53.64%), Query Frame = 0

Query: 15  KELSYKEFVERYMEKNQPVVLT-GLMDDWKACSDWVDENGEANLGFFSTHFGKSRVQVAD 74
           +E+ Y +F + Y+  NQP + +    ++W     WV   G+ NL      F ++ V VA+
Sbjct: 34  REIPYSKFFKNYLIPNQPCMFSKKFTEEWNCRKKWVTAEGKPNLQRLLHEFDETPVPVAN 93

Query: 75  CSTREFTDQKRVEMSVSEFIDQWCKEPIQEHGLTSNNGLTDKSVLYLKDWHFVKEYPNYT 134
           CS +E+    +  M   EFI  W +E IQ      N   + K  LYLKDWH  + +P + 
Sbjct: 94  CSVKEYNANPKQIMPFKEFIQYW-RESIQ------NGHSSPKGCLYLKDWHMQRNFPEHN 153

Query: 135 AYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEICCSDYRFVYMGAKGSWTPLHADVFR 194
            Y TP +   DWLN Y D+  +                DYRFVYMG KGSWTP HADVFR
Sbjct: 154 IYKTPIYFSSDWLNEYWDTIEV---------------DDYRFVYMGPKGSWTPFHADVFR 213

Query: 195 SYSWSANVCGKKQW---------NMKACIYSIFDDIS------ENLFPGFKKATW-LDCT 254
           SYSWSAN+CG+K+W          ++ C  ++  D++      + L+  F++A   L+  
Sbjct: 214 SYSWSANICGRKKWLLYPPGQEDFLRDCHGNLAYDVTAPILQDKGLYAQFEEACQPLEII 273

Query: 255 QEQNEIIFVPSGWYHQVHNLDDTVSINHNWFNSYNLCWVLDLMLRDYNEAKEYIEDIRDI 314
           QE  EIIFVPSGW+HQV+NL+DT+SINHNW N  NL  +   +  + +  +  IE+ RD 
Sbjct: 274 QEAGEIIFVPSGWHHQVYNLEDTISINHNWLNGCNLDIMWQFLQDELSSVQREIEEWRDT 333

Query: 315 CDDFEGLCQRNLAANTGMNFYDFFIFLARFSLANLVVLDTLARDAENVAGSLSPMVQQLA 367
            D +   CQ  + + TG+++ +F  FL   +   +  L++  R+A++    L+  +  L 
Sbjct: 334 MDTWHQHCQVIMKSCTGIDYAEFASFLKTIANNRISFLNSSPRNADSCQDLLAESLCALG 382

BLAST of HG10008693 vs. ExPASy Swiss-Prot
Match: Q5ZHV5 (2-oxoglutarate and iron-dependent oxygenase JMJD4 OS=Gallus gallus OX=9031 GN=JMJD4 PE=2 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 2.4e-57
Identity = 130/396 (32.83%), Postives = 207/396 (52.27%), Query Frame = 0

Query: 9   IDKVNGKELSYKEFVERYMEKNQPVVLT-GLMDDWKACSDWVDENGEANLGFFSTHFGKS 68
           IDK+  +  +Y +F   Y+  NQP V +    D W +  +WV   G+ +       FG++
Sbjct: 32  IDKI--ESFTYSDFFRDYLIPNQPCVFSEKFTDGWGSRRNWVTWGGKPDFDHLLQEFGEA 91

Query: 69  RVQVADCSTREFTDQKRVEMSVSEFIDQWCKEPIQEHGLTSNNGLTDKSVLYLKDWHFVK 128
            V VA+C  +E+    + ++   E+I  W KE I ++G  S+ G      LYLKDWH  +
Sbjct: 92  IVPVANCDVKEYNSNPKEQLPFKEYISYW-KEYI-KNGYRSSRG-----CLYLKDWHLSR 151

Query: 129 EYPNYTAYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEICCSDYRFVYMGAKGSWTPL 188
            +P    Y+TP +   DWLN Y D+               I   DYRFVYMG KGSWTP 
Sbjct: 152 AFPEQDVYTTPVYFSSDWLNEYWDA---------------IAVDDYRFVYMGPKGSWTPF 211

Query: 189 HADVFRSYSWSANVCGKKQW---------NMKACIYSIFDDIS------ENLFPGFKKA- 248
           HADVFRSYSWSAN+CG+K+W          +K C  ++  D++       +++P + ++ 
Sbjct: 212 HADVFRSYSWSANICGRKKWLLYPPGQEDYLKDCHGNLPFDVTAPGLQDRSVYPRYNQSQ 271

Query: 249 TWLDCTQEQNEIIFVPSGWYHQVHNLDDTVSINHNWFNSYNLCWVLDLMLRDYNEAKEYI 308
             ++  QE  EI+F+PSGW+HQV+NL+DT+SINHNW N  N+  +   +  +    +  I
Sbjct: 272 PPVEIVQEAGEIVFIPSGWHHQVYNLEDTISINHNWVNGCNVAIMWCFLQDELAAVQREI 331

Query: 309 EDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARFSLANLVVLDTLARDAENVAGSLSP 368
            + +D  DD+   CQ  + + TG+++ +F+ FL   +   + +L+    D  +   +   
Sbjct: 332 NEWKDPMDDWHLQCQLIMKSCTGIDYKEFYNFLKVIAENRISILENGLDDEASAKNTPKA 391

Query: 369 MVQQLAQNLASIQRIALKMKFLECFSEDQGF-MLDL 387
            +  L   L ++  +   +K L   S ++ F  LDL
Sbjct: 392 AISTLGM-LHAVFDLKRTVKVLTSLSANEDFKKLDL 402

BLAST of HG10008693 vs. ExPASy Swiss-Prot
Match: Q9H9V9 (2-oxoglutarate and iron-dependent oxygenase JMJD4 OS=Homo sapiens OX=9606 GN=JMJD4 PE=1 SV=2)

HSP 1 Score: 203.8 bits (517), Expect = 4.4e-51
Identity = 119/347 (34.29%), Postives = 178/347 (51.30%), Query Frame = 0

Query: 18  SYKEFVERYMEKNQPVVL-TGLMDDWKACSDWVDENGEANLGFFSTHFGKSRVQVADCST 77
           SY +FV  ++  N P V  +     W +   WV   G  +       +G   V VA+C  
Sbjct: 86  SYADFVRGFLLPNLPCVFSSAFTQGWGSRRRWVTPAGRPDFDHLLRTYGDVVVPVANCGV 145

Query: 78  REFTDQKRVEMSVSEFIDQWCKEPIQEHGLTSNNGLTDKSVLYLKDWHFVKEYPNYTAYS 137
           +E+    +  M++ ++I  W KE IQ  G +S  G      LYLKDWH  +++P    ++
Sbjct: 146 QEYNSNPKEHMTLRDYITYW-KEYIQA-GYSSPRG-----CLYLKDWHLCRDFPVEDVFT 205

Query: 138 TPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEICCSDYRFVYMGAKGSWTPLHADVFRSYS 197
            P +   DWLN + D+               +   DYRFVY G  GSW+P HAD+FRS+S
Sbjct: 206 LPVYFSSDWLNEFWDA---------------LDVDDYRFVYAGPAGSWSPFHADIFRSFS 265

Query: 198 WSANVCGKKQW-----NMKACIYSIFDDI----------SENLFPGFKKA-TWLDCTQEQ 257
           WS NVCG+K+W       +  +     ++            +L P  + A   L+ TQE 
Sbjct: 266 WSVNVCGRKKWLLFPPGQEEALRDRHGNLPYDVTSPALCDTHLHPRNQLAGPPLEITQEA 325

Query: 258 NEIIFVPSGWYHQVHNLDDTVSINHNWFNSYNLCWVLDLMLRDYNEAKEYIEDIRDICDD 317
            E++FVPSGW+HQVHNLDDT+SINHNW N +NL  +   + ++    +E + + RD   D
Sbjct: 326 GEMVFVPSGWHHQVHNLDDTISINHNWVNGFNLANMWRFLQQELCAVQEEVSEWRDSMPD 385

Query: 318 FEGLCQRNLAANTGMNFYDFFIFLARFSLANLVVLDTLARDAENVAG 348
           +   CQ  + + +G+NF +F+ FL   +   L+VL   A  AE+ AG
Sbjct: 386 WHHHCQVIMRSCSGINFEEFYHFLKVIAEKRLLVLREAA--AEDGAG 408

BLAST of HG10008693 vs. ExPASy Swiss-Prot
Match: Q8BFT6 (2-oxoglutarate and iron-dependent oxygenase JMJD4 OS=Mus musculus OX=10090 GN=Jmjd4 PE=2 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 4.8e-50
Identity = 113/366 (30.87%), Postives = 188/366 (51.37%), Query Frame = 0

Query: 18  SYKEFVERYMEKNQPVVL-TGLMDDWKACSDWVDENGEANLGFFSTHFGKSRVQVADCST 77
           SY +FV+ ++  N P V  +   + W +   WV   G+ +  +    +G + V VA+C  
Sbjct: 42  SYADFVKGFLLPNLPCVFSSAFTEGWGSRRRWVTSEGKPDFEYLQQKYGDAVVPVANCGV 101

Query: 78  REFTDQKRVEMSVSEFIDQWCKEPIQEHGLTSNNGLTDKSVLYLKDWHFVKE---YPNYT 137
           RE+    +  MS  ++I  W K+ IQ       +  + +  LYLKDWH  ++        
Sbjct: 102 REYNSNPKEHMSFRDYISYW-KDYIQ------GSYSSSRGCLYLKDWHLCRDSLVNDLED 161

Query: 138 AYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEICCSDYRFVYMGAKGSWTPLHADVFR 197
            ++ P +   DWLN + D   +                DYRFVY G +G+W+P HAD+FR
Sbjct: 162 IFTLPVYFSSDWLNEFWDVLNV---------------DDYRFVYAGPRGTWSPFHADIFR 221

Query: 198 SYSWSANVCGKKQW---------NMKACIYSIFDDISE------NLFPGFKKATW-LDCT 257
           S+SWS N+CGKK+W          ++ C  ++  D++       +L+P  +  +  ++  
Sbjct: 222 SFSWSVNICGKKKWLFFPPGEEEALRDCHGNLPYDVTSTELLDTHLYPKIQHHSLPIEVI 281

Query: 258 QEQNEIIFVPSGWYHQVHNLDDTVSINHNWFNSYNLCWVLDLMLRDYNEAKEYIEDIRDI 317
           QE  E++FVPSGW+HQV+NLDDT+SINHNW N  NL  +   + ++    +  +E+ +D 
Sbjct: 282 QEPGEMVFVPSGWHHQVYNLDDTISINHNWVNGCNLPNMWHFLQQELQAVQHEVEEWKDS 341

Query: 318 CDDFEGLCQRNLAANTGMNFYDFFIFLARFSLANLVVLDTLARDAENVAGSLSPMVQQLA 364
             D+   CQ  + + TG+NF +F+ FL   +   L+VL+   +     + SL   +QQ A
Sbjct: 342 MPDWHHHCQVIMKSCTGINFEEFYHFLKVIAEKRLLVLEQGLKGDSGDSRSLDLGLQQAA 385

BLAST of HG10008693 vs. ExPASy Swiss-Prot
Match: Q9VJ97 (2-oxoglutarate and iron-dependent oxygenase JMJD4 homolog OS=Drosophila melanogaster OX=7227 GN=CG7200 PE=2 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 3.1e-49
Identity = 120/362 (33.15%), Postives = 173/362 (47.79%), Query Frame = 0

Query: 9   IDKVNGKELSYKEFVERYMEKNQPVVLTGLMDDWKACSDW-----------VDENGEA-- 68
           I++ +G  L Y +F  RYM KN PV++  + +DW+ C +W           ++ N  A  
Sbjct: 30  IERCSG--LDYNDFFWRYMHKNIPVIIANVSNDWE-CQNWTVGQSSPESRDLNSNPSASS 89

Query: 69  -NLGFFSTHFGKSRVQVADCSTREFTDQKRVEMSVSEFIDQWCKEPIQEHG--------L 128
            N  +  T      V VA+C++  F    ++E++  +++ +W +  I+           +
Sbjct: 90  INFDYLKTKISDGPVPVANCNSSYFNSHTKLELNFHDYLAKW-RSSIESQSSAAWTSAEV 149

Query: 129 TSNNGLTDKSVLYLKDWHFVKEYPNYTAYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKD 188
            SN        LYLKDWH   + P Y  Y  P +   DWLN  L            Q KD
Sbjct: 150 NSNVAPASGDNLYLKDWHLAAQMPGYNFYKVPKYFASDWLNEQL----------IQQGKD 209

Query: 189 EICCSDYRFVYMGAKGSWTPLHADVFRSYSWSANVCGKKQW---------NMKACIYSIF 248
                DYRFVYMG K SWT  HADVF S+SWS N+ G K+W          +   + ++ 
Sbjct: 210 -----DYRFVYMGPKNSWTSYHADVFGSFSWSTNIVGLKKWLIMPPGEELKLNDRLGNLP 269

Query: 249 DDISENLFPGFKKATWLDCTQEQNEIIFVPSGWYHQVHNLDDTVSINHNWFNSYNLCWVL 308
             I E +        +    Q  NE +FVPSGW+HQV NL DT+S+NHNWFN  N+  V 
Sbjct: 270 FSIDEKMLDE-HNVRYYTINQRANEAVFVPSGWFHQVWNLTDTISVNHNWFNGCNISMVW 329

Query: 309 DLMLRDYNEAKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARFSLANLVVLDT 340
             +  +       I D + + D+FE  CQ  L A+ G+N+ D FI L  F  A  +   T
Sbjct: 330 QNLKNNLKAVCNEISDCQQM-DNFEAHCQTMLRASFGINYLD-FIELLEFIAARRLAEGT 369

BLAST of HG10008693 vs. ExPASy TrEMBL
Match: A0A1S3CLV6 (jmjC domain-containing protein 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502391 PE=4 SV=1)

HSP 1 Score: 859.8 bits (2220), Expect = 5.4e-246
Identity = 407/480 (84.79%), Postives = 430/480 (89.58%), Query Frame = 0

Query: 1   MGIEICGRIDKVNGKELSYKEFVERYMEKNQPVVLTGLMDDWKACSDWVDENGEANLGFF 60
           MGIEICG IDKVNGK LSYKEFVERYMEKN+PVVLTGLMDDWKACSDWVDENG+ NL FF
Sbjct: 2   MGIEICGEIDKVNGKGLSYKEFVERYMEKNKPVVLTGLMDDWKACSDWVDENGQPNLSFF 61

Query: 61  STHFGKSRVQVADCSTREFTDQKRVEMSVSEFIDQWCKEPIQEHGLTSNNGLTDKSVLYL 120
           STHFGKSRVQVADC TREFTDQKRVEMSVSEFIDQWCKEPI E+GL SNNGLTD+SVLYL
Sbjct: 62  STHFGKSRVQVADCGTREFTDQKRVEMSVSEFIDQWCKEPIPENGLGSNNGLTDESVLYL 121

Query: 121 KDWHFVKEYPNYTAYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEICCSDYRFVYMGA 180
           KDWHFVKEY NYTAYSTPHFVCDDWLNLYLDSYRMHRDPDSYQE DEI CSDYRFVYMGA
Sbjct: 122 KDWHFVKEYLNYTAYSTPHFVCDDWLNLYLDSYRMHRDPDSYQENDEISCSDYRFVYMGA 181

Query: 181 KGSWTPLHADVFRSYSWSANVCGKKQW--------------NMKACIYSIFDDISENLFP 240
           KGSWTPLHADVFRSYSWSANVCGKKQW              NMKAC+Y+IFDDISENLFP
Sbjct: 182 KGSWTPLHADVFRSYSWSANVCGKKQWFLLPPSQSHLVFDRNMKACVYNIFDDISENLFP 241

Query: 241 GFKKATWLDCTQEQNEIIFVPSGWYHQVHNLDDTVSINHNWFNSYNLCWVLDLMLRDYNE 300
           GFKKATWL+C QE NEIIFVPSGWYHQVHNLDDT+SINHNWFNSYNLCWVLDLMLRDYNE
Sbjct: 242 GFKKATWLECIQEPNEIIFVPSGWYHQVHNLDDTISINHNWFNSYNLCWVLDLMLRDYNE 301

Query: 301 AKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARFSLANLVVLDTLARDAENVA 360
           AKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARFSLAN VVLD L RDAEN+A
Sbjct: 302 AKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARFSLANSVVLDILVRDAENIA 361

Query: 361 --GSLSPMVQQLAQNLASIQRIALKMKFLECFSEDQGFMLDLGETHKDPKFSKLCYSLIQ 420
             GS  P++QQL QNL+SIQ+IALKMK LECFS DQGF+LDL ETHK+P FSKLCYSLIQ
Sbjct: 362 GTGSTFPIIQQLVQNLSSIQQIALKMKSLECFSGDQGFVLDLVETHKNPMFSKLCYSLIQ 421

Query: 421 TCGSIHKQQISSFCPKTIATHDSRTSIIIQNFCDGVSSPKDLVKFIDTVLTKHTNLESPE 465
            C SIH +Q+ S C KT+AT DS T I+IQNFCD VSSP+DLVK+ID VLT+HT+LES E
Sbjct: 422 ICESIHNRQVLSLCSKTVATQDSCTLIVIQNFCDAVSSPQDLVKYIDAVLTEHTSLESSE 481

BLAST of HG10008693 vs. ExPASy TrEMBL
Match: A0A6J1E455 (jmjC domain-containing protein 4 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111429764 PE=4 SV=1)

HSP 1 Score: 855.5 bits (2209), Expect = 1.0e-244
Identity = 410/480 (85.42%), Postives = 429/480 (89.38%), Query Frame = 0

Query: 1   MGIEICGRIDKVNGKELSYKEFVERYMEKNQPVVLTGLMDDWKACSDWVDENGEANLGFF 60
           M IE  GRI+KVNG EL Y+EFVERYMEKNQPVVLTGLMDDWKACSDWVDENG+ NL FF
Sbjct: 2   MAIETSGRIEKVNGNELRYEEFVERYMEKNQPVVLTGLMDDWKACSDWVDENGQPNLRFF 61

Query: 61  STHFGKSRVQVADCSTREFTDQKRVEMSVSEFIDQWCKEPIQ-EHGLTSNNGLTDKSVLY 120
           STHFGKS+VQVADC+TR+FTDQ RVEMSVSEFIDQWCKE +Q E G+TSNNG  DKSVLY
Sbjct: 62  STHFGKSKVQVADCNTRDFTDQMRVEMSVSEFIDQWCKEAVQEERGVTSNNGDIDKSVLY 121

Query: 121 LKDWHFVKEYPNYTAYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEICCSDYRFVYMG 180
           LKDWHFVKEYPNY AYSTPHFV DDWLNLYLDSYRMHRDPDSYQEKDEI CSDYRFVYMG
Sbjct: 122 LKDWHFVKEYPNYAAYSTPHFVRDDWLNLYLDSYRMHRDPDSYQEKDEISCSDYRFVYMG 181

Query: 181 AKGSWTPLHADVFRSYSWSANVCGKKQW--------------NMKACIYSIFD-DISENL 240
           AKGSWTPLHADVFRSYSWSANVCGKKQW              NMKACIY+IFD +ISENL
Sbjct: 182 AKGSWTPLHADVFRSYSWSANVCGKKQWFLLPPSQSHLVFDRNMKACIYNIFDNNISENL 241

Query: 241 FPGFKKATWLDCTQEQNEIIFVPSGWYHQVHNLDDTVSINHNWFNSYNLCWVLDLMLRDY 300
           FPGFKKATWL+C QEQNEIIFVPSGWYHQVHNL+DTVSINHNWFNSYNLCWVLDLM RDY
Sbjct: 242 FPGFKKATWLECIQEQNEIIFVPSGWYHQVHNLEDTVSINHNWFNSYNLCWVLDLMARDY 301

Query: 301 NEAKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARFSLANLVVLDTLARDAEN 360
           NEAKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARF+LANLVVLD LARD EN
Sbjct: 302 NEAKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARFTLANLVVLDNLARDTEN 361

Query: 361 VAGSLSPMVQQLAQNLASIQRIALKMKFLECFSEDQGFMLDLGETHKDPKFSKLCYSLIQ 420
           VAGSLSPMVQQLA NLASI+ I LKMK LECFS DQGFMLDLGETHKDPK SKLCYSL+Q
Sbjct: 362 VAGSLSPMVQQLAHNLASIKNIVLKMKSLECFSGDQGFMLDLGETHKDPKLSKLCYSLVQ 421

Query: 421 TCGSIHKQQISSFCPKTIATHDSRTSIIIQNFCDGVSSPKDLVKFIDTVLTKHTNLESPE 465
            CGSIHKQQISS C KTIATHDSR+  IIQNFCDGVSSP+DLVKFIDTV T+    E+PE
Sbjct: 422 ACGSIHKQQISSLCMKTIATHDSRSLTIIQNFCDGVSSPQDLVKFIDTVSTELAKSEAPE 481

BLAST of HG10008693 vs. ExPASy TrEMBL
Match: A0A6J1JH14 (jmjC domain-containing protein 4 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485632 PE=4 SV=1)

HSP 1 Score: 854.4 bits (2206), Expect = 2.3e-244
Identity = 407/479 (84.97%), Postives = 426/479 (88.94%), Query Frame = 0

Query: 1   MGIEICGRIDKVNGKELSYKEFVERYMEKNQPVVLTGLMDDWKACSDWVDENGEANLGFF 60
           M IE  GRIDKVNG ELSY+EFVERYMEKNQPVVLTGLMD WKACSDWVDENG+ NL FF
Sbjct: 2   MAIETSGRIDKVNGNELSYEEFVERYMEKNQPVVLTGLMDGWKACSDWVDENGQPNLRFF 61

Query: 61  STHFGKSRVQVADCSTREFTDQKRVEMSVSEFIDQWCKEPIQEHGLTSNNGLTDKSVLYL 120
           STHFGKS+VQVADC+TREFTDQKRVEMSVSEFIDQWCKE +QE G+TSNNG  DK VLYL
Sbjct: 62  STHFGKSKVQVADCNTREFTDQKRVEMSVSEFIDQWCKEAVQERGVTSNNGDIDKPVLYL 121

Query: 121 KDWHFVKEYPNYTAYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEICCSDYRFVYMGA 180
           KDWHFVKEYPNY AYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEI CSDYRFVYMGA
Sbjct: 122 KDWHFVKEYPNYAAYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEISCSDYRFVYMGA 181

Query: 181 KGSWTPLHADVFRSYSWSANVCGKKQW--------------NMKACIYSIFD-DISENLF 240
           KGSWTPLHADVFRSYSWSANVCGKKQW              NMKACIY+IFD +ISE LF
Sbjct: 182 KGSWTPLHADVFRSYSWSANVCGKKQWFLLPPSQSHLVFDRNMKACIYNIFDNNISEILF 241

Query: 241 PGFKKATWLDCTQEQNEIIFVPSGWYHQVHNLDDTVSINHNWFNSYNLCWVLDLMLRDYN 300
           PGFKKATWL+C QEQNEIIFVPSGWYHQVHNL+DTVSINHNWFNSYNLCWVLDLM RDYN
Sbjct: 242 PGFKKATWLECIQEQNEIIFVPSGWYHQVHNLEDTVSINHNWFNSYNLCWVLDLMARDYN 301

Query: 301 EAKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARFSLANLVVLDTLARDAENV 360
           EAKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARF+LANLVVLD LARD ENV
Sbjct: 302 EAKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARFTLANLVVLDNLARDTENV 361

Query: 361 AGSLSPMVQQLAQNLASIQRIALKMKFLECFSEDQGFMLDLGETHKDPKFSKLCYSLIQT 420
           AGSLSPMVQQLA NLASI+ I LKMK LECFS DQGF+LDLGETHKDPKFSKLCY L+QT
Sbjct: 362 AGSLSPMVQQLAHNLASIKNIVLKMKSLECFSGDQGFVLDLGETHKDPKFSKLCYFLVQT 421

Query: 421 CGSIHKQQISSFCPKTIATHDSRTSIIIQNFCDGVSSPKDLVKFIDTVLTKHTNLESPE 465
           CG IHKQQIS  C KTIATHDSR S IIQ+FCDGVS+P+DLV FID V T+    E+PE
Sbjct: 422 CGYIHKQQISRLCTKTIATHDSRFSTIIQSFCDGVSTPQDLVTFIDGVSTERAKSEAPE 480

BLAST of HG10008693 vs. ExPASy TrEMBL
Match: A0A6J1E0R3 (jmjC domain-containing protein 4 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111429764 PE=4 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 3.6e-242
Identity = 408/480 (85.00%), Postives = 427/480 (88.96%), Query Frame = 0

Query: 1   MGIEICGRIDKVNGKELSYKEFVERYMEKNQPVVLTGLMDDWKACSDWVDENGEANLGFF 60
           M IE  GRI+KVNG EL Y+EFVERYMEKNQPVVLTGLMDDWKACSDWVDENG+ NL FF
Sbjct: 2   MAIETSGRIEKVNGNELRYEEFVERYMEKNQPVVLTGLMDDWKACSDWVDENGQPNLRFF 61

Query: 61  STHFGKSRVQVADCSTREFTDQKRVEMSVSEFIDQWCKEPIQ-EHGLTSNNGLTDKSVLY 120
           STHFGKS+  VADC+TR+FTDQ RVEMSVSEFIDQWCKE +Q E G+TSNNG  DKSVLY
Sbjct: 62  STHFGKSK--VADCNTRDFTDQMRVEMSVSEFIDQWCKEAVQEERGVTSNNGDIDKSVLY 121

Query: 121 LKDWHFVKEYPNYTAYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEICCSDYRFVYMG 180
           LKDWHFVKEYPNY AYSTPHFV DDWLNLYLDSYRMHRDPDSYQEKDEI CSDYRFVYMG
Sbjct: 122 LKDWHFVKEYPNYAAYSTPHFVRDDWLNLYLDSYRMHRDPDSYQEKDEISCSDYRFVYMG 181

Query: 181 AKGSWTPLHADVFRSYSWSANVCGKKQW--------------NMKACIYSIFD-DISENL 240
           AKGSWTPLHADVFRSYSWSANVCGKKQW              NMKACIY+IFD +ISENL
Sbjct: 182 AKGSWTPLHADVFRSYSWSANVCGKKQWFLLPPSQSHLVFDRNMKACIYNIFDNNISENL 241

Query: 241 FPGFKKATWLDCTQEQNEIIFVPSGWYHQVHNLDDTVSINHNWFNSYNLCWVLDLMLRDY 300
           FPGFKKATWL+C QEQNEIIFVPSGWYHQVHNL+DTVSINHNWFNSYNLCWVLDLM RDY
Sbjct: 242 FPGFKKATWLECIQEQNEIIFVPSGWYHQVHNLEDTVSINHNWFNSYNLCWVLDLMARDY 301

Query: 301 NEAKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARFSLANLVVLDTLARDAEN 360
           NEAKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARF+LANLVVLD LARD EN
Sbjct: 302 NEAKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARFTLANLVVLDNLARDTEN 361

Query: 361 VAGSLSPMVQQLAQNLASIQRIALKMKFLECFSEDQGFMLDLGETHKDPKFSKLCYSLIQ 420
           VAGSLSPMVQQLA NLASI+ I LKMK LECFS DQGFMLDLGETHKDPK SKLCYSL+Q
Sbjct: 362 VAGSLSPMVQQLAHNLASIKNIVLKMKSLECFSGDQGFMLDLGETHKDPKLSKLCYSLVQ 421

Query: 421 TCGSIHKQQISSFCPKTIATHDSRTSIIIQNFCDGVSSPKDLVKFIDTVLTKHTNLESPE 465
            CGSIHKQQISS C KTIATHDSR+  IIQNFCDGVSSP+DLVKFIDTV T+    E+PE
Sbjct: 422 ACGSIHKQQISSLCMKTIATHDSRSLTIIQNFCDGVSSPQDLVKFIDTVSTELAKSEAPE 479

BLAST of HG10008693 vs. ExPASy TrEMBL
Match: A0A6J1JM41 (jmjC domain-containing protein 4 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111485632 PE=4 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 8.1e-242
Identity = 405/479 (84.55%), Postives = 424/479 (88.52%), Query Frame = 0

Query: 1   MGIEICGRIDKVNGKELSYKEFVERYMEKNQPVVLTGLMDDWKACSDWVDENGEANLGFF 60
           M IE  GRIDKVNG ELSY+EFVERYMEKNQPVVLTGLMD WKACSDWVDENG+ NL FF
Sbjct: 2   MAIETSGRIDKVNGNELSYEEFVERYMEKNQPVVLTGLMDGWKACSDWVDENGQPNLRFF 61

Query: 61  STHFGKSRVQVADCSTREFTDQKRVEMSVSEFIDQWCKEPIQEHGLTSNNGLTDKSVLYL 120
           STHFGKS+  VADC+TREFTDQKRVEMSVSEFIDQWCKE +QE G+TSNNG  DK VLYL
Sbjct: 62  STHFGKSK--VADCNTREFTDQKRVEMSVSEFIDQWCKEAVQERGVTSNNGDIDKPVLYL 121

Query: 121 KDWHFVKEYPNYTAYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEICCSDYRFVYMGA 180
           KDWHFVKEYPNY AYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEI CSDYRFVYMGA
Sbjct: 122 KDWHFVKEYPNYAAYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEISCSDYRFVYMGA 181

Query: 181 KGSWTPLHADVFRSYSWSANVCGKKQW--------------NMKACIYSIFD-DISENLF 240
           KGSWTPLHADVFRSYSWSANVCGKKQW              NMKACIY+IFD +ISE LF
Sbjct: 182 KGSWTPLHADVFRSYSWSANVCGKKQWFLLPPSQSHLVFDRNMKACIYNIFDNNISEILF 241

Query: 241 PGFKKATWLDCTQEQNEIIFVPSGWYHQVHNLDDTVSINHNWFNSYNLCWVLDLMLRDYN 300
           PGFKKATWL+C QEQNEIIFVPSGWYHQVHNL+DTVSINHNWFNSYNLCWVLDLM RDYN
Sbjct: 242 PGFKKATWLECIQEQNEIIFVPSGWYHQVHNLEDTVSINHNWFNSYNLCWVLDLMARDYN 301

Query: 301 EAKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARFSLANLVVLDTLARDAENV 360
           EAKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARF+LANLVVLD LARD ENV
Sbjct: 302 EAKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARFTLANLVVLDNLARDTENV 361

Query: 361 AGSLSPMVQQLAQNLASIQRIALKMKFLECFSEDQGFMLDLGETHKDPKFSKLCYSLIQT 420
           AGSLSPMVQQLA NLASI+ I LKMK LECFS DQGF+LDLGETHKDPKFSKLCY L+QT
Sbjct: 362 AGSLSPMVQQLAHNLASIKNIVLKMKSLECFSGDQGFVLDLGETHKDPKFSKLCYFLVQT 421

Query: 421 CGSIHKQQISSFCPKTIATHDSRTSIIIQNFCDGVSSPKDLVKFIDTVLTKHTNLESPE 465
           CG IHKQQIS  C KTIATHDSR S IIQ+FCDGVS+P+DLV FID V T+    E+PE
Sbjct: 422 CGYIHKQQISRLCTKTIATHDSRFSTIIQSFCDGVSTPQDLVTFIDGVSTERAKSEAPE 478

BLAST of HG10008693 vs. TAIR 10
Match: AT5G63080.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 540.4 bits (1391), Expect = 1.4e-153
Identity = 259/466 (55.58%), Postives = 331/466 (71.03%), Query Frame = 0

Query: 1   MGIEICGRIDKVNGKELSYKEFVERYMEKNQPVVLTGLMDDWKACSDWVDENGEANLGFF 60
           MGI+I G+I+++NGKELSY +F ERY+ KNQPVV++ L +DW+A  DWV ENG  NL  F
Sbjct: 1   MGIQIIGQIERINGKELSYGDFAERYLAKNQPVVISDLTEDWRAREDWVSENGNPNLHVF 60

Query: 61  STHFGKSRVQVADCSTREFTDQKRVEMSVSEFIDQWCKEPIQEHGLTSNNGLTDKSVLYL 120
           +THFGKSRVQVADC TREFTDQKR+EMSV+EF++QW           +N    ++SVLYL
Sbjct: 61  ATHFGKSRVQVADCDTREFTDQKRLEMSVTEFVEQW-----------TNKDSIEESVLYL 120

Query: 121 KDWHFVKEYPNYTAYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEICCSDYRFVYMGA 180
           KDWHFVKEYP+YTAY TP    DDWLN+YLD+Y+MH D DS+Q+ D+I CSDYRFVYMG 
Sbjct: 121 KDWHFVKEYPDYTAYQTPPLFSDDWLNVYLDNYQMHEDRDSFQKYDQISCSDYRFVYMGG 180

Query: 181 KGSWTPLHADVFRSYSWSANVCGKKQW--------------NMKACIYSIFDDISENLFP 240
           KGSWTPLHADVFRSYSWSANVCGKK+W               MK C+Y IF++++E  FP
Sbjct: 181 KGSWTPLHADVFRSYSWSANVCGKKRWLFLPPPQSHLVYDRYMKNCVYDIFEEVNETKFP 240

Query: 241 GFKKATWLDCTQEQNEIIFVPSGWYHQVHNLDDTVSINHNWFNSYNLCWVLDLMLRDYNE 300
           GFKK TWL+C QE  EIIFVPSGW+HQV+NL+DT+SINHNW N+YNL WV DL+ +DY +
Sbjct: 241 GFKKTTWLECIQEPGEIIFVPSGWHHQVYNLEDTISINHNWLNAYNLSWVWDLLWKDYKD 300

Query: 301 AKEYIEDIRDICDDFEGLCQRNLAANTGMNFYDFFIFLARFSLANLVVLDTLARDAENVA 360
            +E IEDIRDICDDFE +CQRNLAANTGMN  DFF+F++RFSL N+V+L + +   +N+ 
Sbjct: 301 TEESIEDIRDICDDFEAICQRNLAANTGMNLNDFFLFMSRFSLGNMVLLQSYSDKHKNLN 360

Query: 361 GSLSPMVQQLAQNLASIQRIALKMKFLECFSEDQGFMLDLGETHKDPKFSKLCYSLIQTC 420
                M Q L  NL++I ++ +KM      + ++ + LDL ET +DP+F +    + +T 
Sbjct: 361 SCSLAMAQNLLMNLSTILKVMMKMISAGGVTAEEVY-LDLRETLEDPQFLRFVRDMGRTY 420

Query: 421 GSIHKQQISSF-CPKTIATHDSRTSIIIQNFCDGVSSPKDLVKFID 452
             IH ++   F   K +    S  +      C    SPKDLV+ I+
Sbjct: 421 ARIHMEEEDQFLSSKELLQKLSGLAGPNMQIC----SPKDLVEMIN 450

BLAST of HG10008693 vs. TAIR 10
Match: AT1G78280.1 (transferases, transferring glycosyl groups )

HSP 1 Score: 84.0 bits (206), Expect = 3.6e-16
Identity = 68/284 (23.94%), Postives = 116/284 (40.85%), Query Frame = 0

Query: 15  KELSYKEFVERYMEKNQPVVLTGLMDDWKACSDWVDENGEANLGFFSTHFGKSRVQVADC 74
           + +S  EF + Y +  +PV+L+GL D W A + W  +         S  +G+   +++  
Sbjct: 131 RNISLDEFSKEY-DAKKPVLLSGLADSWPASNTWTIDQ-------LSEKYGEVPFRISQR 190

Query: 75  STREFTDQKRVEMSVSEFIDQWCKEPIQEHGLTSNNGLTDKSVLYLKDWHFVKEYPNYTA 134
           S        ++ M   ++I     +              D+  LY+ D  F +  P    
Sbjct: 191 S------PNKISMKFKDYIAYMKTQ-------------RDEDPLYVFDDKFGEAAPELLK 250

Query: 135 -YSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEICCSDYRFVYMGAKGSWTPLHADVFR 194
            YS PH   +DW  + LD  +  R P             YR++ +G + S    H D   
Sbjct: 251 DYSVPHLFQEDWFEI-LD--KESRPP-------------YRWLIVGPERSGASWHVDPAL 310

Query: 195 SYSWSANVCGKKQW--------NMKACIYSIFDDISENLFPGFKKATWLD---------- 254
           + +W+  +CG+K+W         +   ++   DD   ++        WLD          
Sbjct: 311 TSAWNTLLCGRKRWALYPPGKVPLGVTVHVNEDDGDVSIDTPSSLQWWLDYYPLLADEDK 370

Query: 255 ---CTQEQNEIIFVPSGWYHQVHNLDDTVSINHNWFNSYNLCWV 277
              CT    E I+VPSGW+H + NL+ TV++  N+ N  N  +V
Sbjct: 371 PIECTLLPGETIYVPSGWWHCILNLEPTVAVTQNFVNKENFGFV 371

BLAST of HG10008693 vs. TAIR 10
Match: AT5G06550.1 (CONTAINS InterPro DOMAIN/s: Transcription factor jumonji/aspartyl beta-hydroxylase (InterPro:IPR003347), F-box domain, Skp2-like (InterPro:IPR022364), Transcription factor jumonji (InterPro:IPR013129); BEST Arabidopsis thaliana protein match is: transferases, transferring glycosyl groups (TAIR:AT1G78280.1); Has 1762 Blast hits to 1747 proteins in 292 species: Archae - 0; Bacteria - 297; Metazoa - 877; Fungi - 168; Plants - 221; Viruses - 0; Other Eukaryotes - 199 (source: NCBI BLink). )

HSP 1 Score: 84.0 bits (206), Expect = 3.6e-16
Identity = 72/309 (23.30%), Postives = 130/309 (42.07%), Query Frame = 0

Query: 9   IDKVNGKELSYKEFVERYMEKNQPVVLTGLMDDWKACSDWVDENGEANLGFFSTHFGKSR 68
           I +V G  +S ++F+ ++ E N+PV+L G +D W A   W       +  + +   G   
Sbjct: 194 ITRVRG--ISVEDFITKFEEPNKPVLLEGCLDGWPAIEKW-------SRDYLTKVVGDVE 253

Query: 69  VQVADCSTREFTDQKRVEMSVSEFIDQWCKEPIQEHGLTSNNGLTDKSVLYLKDWHFVKE 128
             V             VEM + ++                ++G  ++  LYL D  F ++
Sbjct: 254 FAVGP-----------VEMKLEKY-------------FRYSDGAREERPLYLFDPKFAEK 313

Query: 129 YPNY-TAYSTPHFVCDDWLNLYLDSYRMHRDPDSYQEKDEICCSDYRFVYMGAKGSWTPL 188
            P   + Y  P +  +D   + L + R                 DYR++ +G  GS +  
Sbjct: 314 VPVLDSEYDVPVYFREDLFGV-LGNER----------------PDYRWIIIGPAGSGSSF 373

Query: 189 HADVFRSYSWSANVCGKKQWNM------------------KACIYSIFDDISENLFPGFK 248
           H D   + +W+A + G K+W +                   AC  SI +    N +   K
Sbjct: 374 HIDPNSTSAWNAVITGSKKWVLFPPDVVPPGVHPSPDGAEVACPVSIIEWFM-NFYDDTK 433

Query: 249 --KATWLDCTQEQNEIIFVPSGWYHQVHNLDDTVSINHNWFNSYNLCWVLDLMLRDYNEA 297
             +   ++C  +  E++FVP+GW+H V NL+++++I  N+ +  NL  VL+ + +    A
Sbjct: 434 DWEKKPIECICKAGEVMFVPNGWWHLVINLEESIAITQNYASRSNLLNVLEFLKKP--NA 449

BLAST of HG10008693 vs. TAIR 10
Match: AT3G20810.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 58.2 bits (139), Expect = 2.1e-08
Identity = 38/132 (28.79%), Postives = 60/132 (45.45%), Query Frame = 0

Query: 165 KDEICCSDYRFV----------YMGAKGSWTPLHADVFRSYSWSANVCGKKQWNMKACI- 224
           +D+IC  DY FV          + G  G+ TPLH D    ++  A V GKK   +     
Sbjct: 293 RDDICIPDYCFVGGGELQSLNAWFGPAGTVTPLHHD--PHHNILAQVVGKKYIRLYPSFL 352

Query: 225 ------YS----------IFDDISENLFPGFKKATWLDCTQEQNEIIFVPSGWYHQVHNL 270
                 YS            D+I E  FP   +  ++DC  E+ E++++P  W+H V +L
Sbjct: 353 QDELYPYSETMLCNSSQVDLDNIDETEFPKAMELEFMDCILEEGEMLYIPPKWWHYVRSL 412

BLAST of HG10008693 vs. TAIR 10
Match: AT3G20810.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 58.2 bits (139), Expect = 2.1e-08
Identity = 38/132 (28.79%), Postives = 60/132 (45.45%), Query Frame = 0

Query: 165 KDEICCSDYRFV----------YMGAKGSWTPLHADVFRSYSWSANVCGKKQWNMKACI- 224
           +D+IC  DY FV          + G  G+ TPLH D    ++  A V GKK   +     
Sbjct: 282 RDDICIPDYCFVGGGELQSLNAWFGPAGTVTPLHHD--PHHNILAQVVGKKYIRLYPSFL 341

Query: 225 ------YS----------IFDDISENLFPGFKKATWLDCTQEQNEIIFVPSGWYHQVHNL 270
                 YS            D+I E  FP   +  ++DC  E+ E++++P  W+H V +L
Sbjct: 342 QDELYPYSETMLCNSSQVDLDNIDETEFPKAMELEFMDCILEEGEMLYIPPKWWHYVRSL 401

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038879491.12.0e-26391.212-oxoglutarate and iron-dependent oxygenase JMJD4 isoform X2 [Benincasa hispida][more]
XP_038879489.11.1e-25885.832-oxoglutarate and iron-dependent oxygenase JMJD4 isoform X1 [Benincasa hispida]... [more]
XP_004138273.23.1e-25687.922-oxoglutarate and iron-dependent oxygenase JMJD4 isoform X2 [Cucumis sativus] >... [more]
XP_031740132.18.0e-25285.372-oxoglutarate and iron-dependent oxygenase JMJD4 isoform X1 [Cucumis sativus][more]
XP_023516598.15.7e-25086.64jmjC domain-containing protein 4 isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q08BY51.8e-6035.312-oxoglutarate and iron-dependent oxygenase JMJD4 OS=Danio rerio OX=7955 GN=jmjd... [more]
Q5ZHV52.4e-5732.832-oxoglutarate and iron-dependent oxygenase JMJD4 OS=Gallus gallus OX=9031 GN=JM... [more]
Q9H9V94.4e-5134.292-oxoglutarate and iron-dependent oxygenase JMJD4 OS=Homo sapiens OX=9606 GN=JMJ... [more]
Q8BFT64.8e-5030.872-oxoglutarate and iron-dependent oxygenase JMJD4 OS=Mus musculus OX=10090 GN=Jm... [more]
Q9VJ973.1e-4933.152-oxoglutarate and iron-dependent oxygenase JMJD4 homolog OS=Drosophila melanoga... [more]
Match NameE-valueIdentityDescription
A0A1S3CLV65.4e-24684.79jmjC domain-containing protein 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502... [more]
A0A6J1E4551.0e-24485.42jmjC domain-containing protein 4 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1JH142.3e-24484.97jmjC domain-containing protein 4 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1E0R33.6e-24285.00jmjC domain-containing protein 4 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1JM418.1e-24284.55jmjC domain-containing protein 4 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT5G63080.11.4e-15355.582-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G78280.13.6e-1623.94transferases, transferring glycosyl groups [more]
AT5G06550.13.6e-1623.30CONTAINS InterPro DOMAIN/s: Transcription factor jumonji/aspartyl beta-hydroxyla... [more]
AT3G20810.22.1e-0828.792-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G20810.12.1e-0828.792-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003347JmjC domainSMARTSM00558cupin_9coord: 144..282
e-value: 1.1E-5
score: 34.9
IPR003347JmjC domainPFAMPF02373JmjCcoord: 234..267
e-value: 5.6E-9
score: 36.4
IPR003347JmjC domainPROSITEPS51184JMJCcoord: 126..284
score: 26.750746
NoneNo IPR availableGENE3D2.60.120.650Cupincoord: 3..269
e-value: 5.5E-63
score: 214.9
NoneNo IPR availablePANTHERPTHR12480ARGININE DEMETHYLASE AND LYSYL-HYDROXYLASE JMJDcoord: 7..457
NoneNo IPR availablePANTHERPTHR12480:SF62-OXOGLUTARATE AND IRON-DEPENDENT OXYGENASE JMJD4coord: 7..457
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 9..296
IPR041667Cupin-like domain 8PFAMPF13621Cupin_8coord: 21..207
e-value: 6.7E-10
score: 39.2

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10008693.1HG10008693.1mRNA