HG10008657 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10008657
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionUbiquitin-conjugating enzyme, E2
LocationChr10: 24953336 .. 24955583 (+)
RNA-Seq ExpressionHG10008657
SyntenyHG10008657
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCTAGGTAAGTTCAACTGTATGCATTTATTTATTGCCCTTGAATGTAGTGATGATTACAGTGAGCCGTGACCGAGAGTGACCATACTTGGTGTTGTAATCATGGTTGCAGCAAAAGTAGCGGTTATCTTGTTGTTATGTGCTAGAAAACATATTGGGCATGCTGGCTTAAAACTGGCCCAGCATTGGGGTGATTTCAGGTTTTGAGTTTGACCATTGTTCACTGTTGTGCCTGTAGACCATTGAATGGCTAATGTCTGCACTTGAGGTTGTGCTATCTCTTAATAAAAAATGTGGAACTGAGGTCCTTTTAAGGGTTGATAAAATACTATGTGGTAACATGAATTAGATTCCTAATATTGTATGATCATCTCTGGAATTCTATTACTAACTCATGCAACAGTGATGGCCATAAATTTCTGTTGGTTTATCTTGTGGTTCATGTTACATTGGCTTATGTTGTCCAGGATGATGAGTGACTACAATGTGGAGATGATAAATGATGGACTCAGTGAATTCAATGTGGAGTTCCATGGTCCCAAAGAAAGTATTACTCGACTTTCACCTCTGTCCAATATGCATTTCATAGTCATTGATGGAATAGCAATTATTCGTTTGTGGGTGTGTATGAGCATTATTGGATTGCAAATGAACAATTTAGCAAACTTGCCGGCAGTCTAAAGCCAATTCTTTTGTCTTGTTTGGAGTCTCCTGAAAATTTATCCTTGAAGTGAGGTGTTTCTACTGCTTTCCTGTGTAATTTATGGACACTTGATGCTGTTTACTGTTTCACTTAACATTGATGAGTAAGAGCTGCAGCCTGTTCCTAAAGAGTTTTAATCCATGAAGAAAACTGTGCTTTTGGCTGTTCAAAAGATTGTGAAAGTACATCAGCATGTGGTTTAGTTACTATTCGGATTCCACTTAGCTCTAAAAGGTATAAAGTTTGGATCATAGATGACTTCAAACTCTGAATAGAAATGAACTTTTTGGTTTTATTTACAAGGAAATGTCAACTGGAAACATAATATGCATATAGTTTAGCTTCATGTTTTCATTTAATGAATTATTTTTCACCACATCTGGGTCTAGAGCTGCAATAAGTTAATTATCTTTCGATAAGTATTTTCTTACTCAAGTTTCATTATCCTTGCAGGCTTATATGAAGCTGGCGTTTGGAAAATCCGTGTTGAGCTACCTGATGCATATCCATATAAGTCTCCTTCAATTGGTTTTGTGAACAAGATATTCCACCCAAATGTTGACGAACTGTAAGGAAATGTTTTTTTTTTTCTTTCTAAAACCATCAAATGAAATTGATTGAATGCTTCACCTATTCATAGCAAAGTATTTAACAGGTAACTTTTACCTGCAGATCTGGTTCTGTGTGCTTGGATGTGATTAATCAGTCTTGGAGTCCGATGTTCGGTAATTATCTGCCGCCCCAATCCTCACTAAATCTCTATTAACAATTTACTTGTTTCCAGAGCATTTTTTGCATGTGTTACTGTGATAAGATGTTATTGCACAATAGCTTTTGATGTGTTTGGAAATATAATTTAACATCCATGGGACCATATGAATTGTATTGAGACCCTCAAAATATAGGACTAAACACTTTACTACATATATTTGGATTTGGATTTGCATATGTTTGTGTTTATTCTTATCTCTTTCTGTTGGGTTTTGCAGATCTCTTAAATGTTTTTGAGGTTTTTCTTCCCCAGCTTCTCCTTTATCCCAATCCTTCGGACCCCCTTAACGGTGACGCAGCGTCGCTTATGATGAAGGACCGGAAGCAGTATGATGAAAAAGTTAAAGGTAAATATATCTCACTGAACAACTTTTCTAAATCCCCTATACCACAACCTTCTCCATTTAATTTAAAATGAACTTTCCCCCTGAAAATCATCAAAGGTTGGAAGTCAAATTCCCAATGTAAGTGAATAAACTGTCTTGCAAACTTTGTCAACAACCATTTTTCCCAGTTTAAAGTACAAATGTTATTCTGCTTCTTAAATGTTGAGATGACAGAGATTTTGGCCCTTTTTTTGGTCCTAACAATTACTTTCTTAAATGATAACTCAATATCTACTTCCATATGCATACAGAGTACTGTGACCGATATGCGAAGAAGGAGAACGACATTAAATGCACGGCAGAAGATGAAAGTGAAGAGGATGAAATCAGCGATAACGAAAGTGGTTCAAGCGACGACGATGTTGCTGGACATGCTGATCCCTAA

mRNA sequence

ATGAAGCTAGGCTTATATGAAGCTGGCGTTTGGAAAATCCGTGTTGAGCTACCTGATGCATATCCATATAAGTCTCCTTCAATTGGTTTTGTGAACAAGATATTCCACCCAAATGTTGACGAACTATCTGGTTCTGTGTGCTTGGATGTGATTAATCAGTCTTGGAGTCCGATGTTCGATCTCTTAAATGTTTTTGAGGTTTTTCTTCCCCAGCTTCTCCTTTATCCCAATCCTTCGGACCCCCTTAACGGTGACGCAGCGTCGCTTATGATGAAGGACCGGAAGCAGTATGATGAAAAAGTTAAAGAGTACTGTGACCGATATGCGAAGAAGGAGAACGACATTAAATGCACGGCAGAAGATGAAAGTGAAGAGGATGAAATCAGCGATAACGAAAGTGGTTCAAGCGACGACGATGTTGCTGGACATGCTGATCCCTAA

Coding sequence (CDS)

ATGAAGCTAGGCTTATATGAAGCTGGCGTTTGGAAAATCCGTGTTGAGCTACCTGATGCATATCCATATAAGTCTCCTTCAATTGGTTTTGTGAACAAGATATTCCACCCAAATGTTGACGAACTATCTGGTTCTGTGTGCTTGGATGTGATTAATCAGTCTTGGAGTCCGATGTTCGATCTCTTAAATGTTTTTGAGGTTTTTCTTCCCCAGCTTCTCCTTTATCCCAATCCTTCGGACCCCCTTAACGGTGACGCAGCGTCGCTTATGATGAAGGACCGGAAGCAGTATGATGAAAAAGTTAAAGAGTACTGTGACCGATATGCGAAGAAGGAGAACGACATTAAATGCACGGCAGAAGATGAAAGTGAAGAGGATGAAATCAGCGATAACGAAAGTGGTTCAAGCGACGACGATGTTGCTGGACATGCTGATCCCTAA

Protein sequence

MKLGLYEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDLLNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCDRYAKKENDIKCTAEDESEEDEISDNESGSSDDDVAGHADP
Homology
[Close]
BLAST of HG10008657 vs. NCBI nr
Match: XP_038880248.1 (ubiquitin-conjugating enzyme E2-23 kDa-like [Benincasa hispida])

HSP 1 Score: 283.5 bits (724), Expect = 1.0e-72
Identity = 140/145 (96.55%), Postives = 142/145 (97.93%), Query Frame = 0 

Query: 2   KLGLYEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL 61
           K  LYE GVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL
Sbjct: 40  KESLYETGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL 99

Query: 62  LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCDRYAKKENDIKCTAED 121
           LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYC+RYAKKENDIKCTAED
Sbjct: 100 LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCERYAKKENDIKCTAED 159

Query: 122 ESEEDEISDNESGSSDDDVAGHADP 147
           ESEEDEIS+NESGSSDDDVAGHADP
Sbjct: 160 ESEEDEISNNESGSSDDDVAGHADP 184

[Close]
BLAST of HG10008657 vs. NCBI nr
Match: XP_022988415.1 (ubiquitin-conjugating enzyme E2-23 kDa-like [Cucurbita maxima])

HSP 1 Score: 282.0 bits (720), Expect = 3.0e-72
Identity = 138/145 (95.17%), Postives = 142/145 (97.93%), Query Frame = 0 

Query: 2   KLGLYEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL 61
           K  LYE GVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL
Sbjct: 40  KQSLYETGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL 99

Query: 62  LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCDRYAKKENDIKCTAED 121
           LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYC+R+AKKENDIKCT+ED
Sbjct: 100 LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCERFAKKENDIKCTSED 159

Query: 122 ESEEDEISDNESGSSDDDVAGHADP 147
           ESEEDE+SDNESGSSDDDVAGHADP
Sbjct: 160 ESEEDEMSDNESGSSDDDVAGHADP 184

[Close]
BLAST of HG10008657 vs. NCBI nr
Match: XP_022135179.1 (ubiquitin-conjugating enzyme E2-23 kDa-like isoform X1 [Momordica charantia] >XP_022135180.1 ubiquitin-conjugating enzyme E2-23 kDa-like isoform X1 [Momordica charantia])

HSP 1 Score: 279.3 bits (713), Expect = 2.0e-71
Identity = 138/145 (95.17%), Postives = 142/145 (97.93%), Query Frame = 0 

Query: 2   KLGLYEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL 61
           K  LYEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL
Sbjct: 40  KESLYEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL 99

Query: 62  LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCDRYAKKENDIKCTAED 121
           LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYC+RYAKKEN IKCTAED
Sbjct: 100 LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCERYAKKENGIKCTAED 159

Query: 122 ESEEDEISDNESGSSDDDVAGHADP 147
           ESEEDEIS+++SGSSDDDVAGHADP
Sbjct: 160 ESEEDEISNDDSGSSDDDVAGHADP 184

[Close]
BLAST of HG10008657 vs. NCBI nr
Match: XP_022921689.1 (ubiquitin-conjugating enzyme E2-23 kDa-like isoform X2 [Cucurbita moschata] >XP_022921690.1 ubiquitin-conjugating enzyme E2-23 kDa-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 277.3 bits (708), Expect = 7.4e-71
Identity = 138/146 (94.52%), Postives = 142/146 (97.26%), Query Frame = 0 

Query: 2   KLGLYEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL 61
           K  LYE GVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL
Sbjct: 40  KQSLYETGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL 99

Query: 62  LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCDRYAKKENDIKCTAED 121
           LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYC+R+AKKENDIKCT+ED
Sbjct: 100 LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCERFAKKENDIKCTSED 159

Query: 122 ESEEDEISDNESGSSDDD-VAGHADP 147
           ESEEDE+SDNESGSSDDD VAGHADP
Sbjct: 160 ESEEDEMSDNESGSSDDDNVAGHADP 185

[Close]
BLAST of HG10008657 vs. NCBI nr
Match: XP_022921686.1 (ubiquitin-conjugating enzyme E2-23 kDa-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 277.3 bits (708), Expect = 7.4e-71
Identity = 138/146 (94.52%), Postives = 142/146 (97.26%), Query Frame = 0 

Query: 2   KLGLYEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL 61
           K  LYE GVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL
Sbjct: 61  KQSLYETGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL 120

Query: 62  LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCDRYAKKENDIKCTAED 121
           LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYC+R+AKKENDIKCT+ED
Sbjct: 121 LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCERFAKKENDIKCTSED 180

Query: 122 ESEEDEISDNESGSSDDD-VAGHADP 147
           ESEEDE+SDNESGSSDDD VAGHADP
Sbjct: 181 ESEEDEMSDNESGSSDDDNVAGHADP 206

[Close]
BLAST of HG10008657 vs. ExPASy Swiss-Prot
Match: P16577 (Ubiquitin-conjugating enzyme E2-23 kDa OS=Triticum aestivum OX=4565 GN=UBC4 PE=1 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 6.6e-54
Identity = 103/146 (70.55%), Postives = 123/146 (84.25%), Query Frame = 0 

Query: 2   KLGLYEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL 61
           K  +Y+ GVWK+RVEL +AYPYKSPSIGF NKI+HPNVDE+SGSVCLDVINQ+WSPMFDL
Sbjct: 40  KDSIYQGGVWKVRVELTEAYPYKSPSIGFTNKIYHPNVDEMSGSVCLDVINQTWSPMFDL 99

Query: 62  LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCDRYAKKENDIKCTAED 121
           +N+FEVFLPQLLLYPNPSDPLNG+AASLMM+D+  Y+ KVKEYC+RYAK E DI    E+
Sbjct: 100 VNIFEVFLPQLLLYPNPSDPLNGEAASLMMRDKNAYENKVKEYCERYAKPE-DISPEEEE 159

Query: 122 ESEEDEISDNES-GSSDDDVAGHADP 147
           E  ++E+SD E   S D+ + GHADP
Sbjct: 160 EESDEELSDAEGYDSGDEAIMGHADP 184

[Close]
BLAST of HG10008657 vs. ExPASy Swiss-Prot
Match: P42748 (Ubiquitin-conjugating enzyme E2 4 OS=Arabidopsis thaliana OX=3702 GN=UBC4 PE=2 SV=2)

HSP 1 Score: 206.5 bits (524), Expect = 2.1e-52
Identity = 103/148 (69.59%), Postives = 124/148 (83.78%), Query Frame = 0 

Query: 2   KLGLYEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL 61
           K  LY+ GVWKIRVELPDAYPYKSPS+GF+ KI+HPNVDELSGSVCLDVINQ+WSPMFDL
Sbjct: 40  KDSLYQGGVWKIRVELPDAYPYKSPSVGFITKIYHPNVDELSGSVCLDVINQTWSPMFDL 99

Query: 62  LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCDRYAK--KENDIKCTA 121
           +NVFE FLPQLLLYPNPSDPLNG+AA+LMM+DR  Y+++VKEYC++YAK  + ++ K + 
Sbjct: 100 VNVFETFLPQLLLYPNPSDPLNGEAAALMMRDRPAYEQRVKEYCEKYAKPGEGSEDKSSD 159

Query: 122 EDESEEDEISDNESGSSDD-DVAGHADP 147
           E+ SEE+  SDNE    DD  +AG  DP
Sbjct: 160 EELSEEEYGSDNEDDDDDDVAIAGKPDP 187

[Close]
BLAST of HG10008657 vs. ExPASy Swiss-Prot
Match: P42749 (Ubiquitin-conjugating enzyme E2 5 OS=Arabidopsis thaliana OX=3702 GN=UBC5 PE=2 SV=2)

HSP 1 Score: 205.3 bits (521), Expect = 4.7e-52
Identity = 101/150 (67.33%), Postives = 122/150 (81.33%), Query Frame = 0 

Query: 2   KLGLYEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL 61
           K  +YE GVWKIRVELPDAYPYKSPS+GF+ KI+HPNVDE+SGSVCLDVINQ+WSPMFDL
Sbjct: 40  KDSIYEGGVWKIRVELPDAYPYKSPSVGFITKIYHPNVDEMSGSVCLDVINQTWSPMFDL 99

Query: 62  LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCDRYAKKENDIKCTAED 121
           +NVFE FLPQLLLYPNPSDPLNG+AA+LMM+DR  Y+++VKEYC++YAK   D     E+
Sbjct: 100 VNVFETFLPQLLLYPNPSDPLNGEAAALMMRDRPTYEQRVKEYCEKYAKPRAD----TEE 159

Query: 122 ESEEDEISDNESGSSDDD-----VAGHADP 147
            S +DE+S++E  S  DD     +AG  DP
Sbjct: 160 MSSDDEMSEDEYASDGDDEDDVAIAGKLDP 185

[Close]
BLAST of HG10008657 vs. ExPASy Swiss-Prot
Match: P42750 (Ubiquitin-conjugating enzyme E2 6 OS=Arabidopsis thaliana OX=3702 GN=UBC6 PE=2 SV=2)

HSP 1 Score: 201.1 bits (510), Expect = 8.9e-51
Identity = 96/142 (67.61%), Postives = 119/142 (83.80%), Query Frame = 0 

Query: 5   LYEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDLLNV 64
           LY+ GVWKI+VELP+AYPYKSPS+GFVNKI+HPNVDE SG+VCLDVINQ+WSPMFDL+NV
Sbjct: 43  LYQGGVWKIKVELPEAYPYKSPSVGFVNKIYHPNVDESSGAVCLDVINQTWSPMFDLINV 102

Query: 65  FEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCDRYAKKENDIKCTAEDESE 124
           FE FLPQLLLYPNPSDP NG+AASL+M+DR  Y+ KVKEYC++YAK E  +    +D+S 
Sbjct: 103 FESFLPQLLLYPNPSDPFNGEAASLLMRDRAAYELKVKEYCEKYAKPEEILSDDDDDDSM 162

Query: 125 EDEISDNESGSSDDDVAGHADP 147
            ++ SD++    DD++ G ADP
Sbjct: 163 SEDGSDSDD-DDDDEIVGKADP 183

[Close]
BLAST of HG10008657 vs. ExPASy Swiss-Prot
Match: Q9P7R4 (Ubiquitin-conjugating enzyme E2 8 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ubc8 PE=3 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 6.2e-44
Identity = 89/142 (62.68%), Postives = 111/142 (78.17%), Query Frame = 0 

Query: 6   YEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDLLNVF 65
           Y  G+WK+ VELP  YP+KSPSIGFVN+IFHPN+DELSGSVCLDVINQ+WSPMFD++N+F
Sbjct: 44  YSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSVCLDVINQTWSPMFDMINIF 103

Query: 66  EVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCDRYAKKENDIKCTAEDESEE 125
           EVFLPQLL YPN SDPLNG+AA+L++++   Y  KV++Y  RYA KE D   T  D S++
Sbjct: 104 EVFLPQLLRYPNASDPLNGEAAALLLREPNTYYAKVRDYIARYANKE-DADITLNDSSDD 163

Query: 126 DEIS--DNESGSSDDDVAGHAD 146
           D +S  D ESG   D++AG  D
Sbjct: 164 DTMSSIDTESG---DEIAGPMD 181

[Close]
BLAST of HG10008657 vs. ExPASy TrEMBL
Match: A0A6J1JJH6 (ubiquitin-conjugating enzyme E2-23 kDa-like OS=Cucurbita maxima OX=3661 GN=LOC111485665 PE=3 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 1.5e-72
Identity = 138/145 (95.17%), Postives = 142/145 (97.93%), Query Frame = 0 

Query: 2   KLGLYEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL 61
           K  LYE GVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL
Sbjct: 40  KQSLYETGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL 99

Query: 62  LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCDRYAKKENDIKCTAED 121
           LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYC+R+AKKENDIKCT+ED
Sbjct: 100 LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCERFAKKENDIKCTSED 159

Query: 122 ESEEDEISDNESGSSDDDVAGHADP 147
           ESEEDE+SDNESGSSDDDVAGHADP
Sbjct: 160 ESEEDEMSDNESGSSDDDVAGHADP 184

[Close]
BLAST of HG10008657 vs. ExPASy TrEMBL
Match: A0A6J1C0G2 (ubiquitin-conjugating enzyme E2-23 kDa-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007210 PE=3 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 9.5e-72
Identity = 138/145 (95.17%), Postives = 142/145 (97.93%), Query Frame = 0 

Query: 2   KLGLYEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL 61
           K  LYEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL
Sbjct: 40  KESLYEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL 99

Query: 62  LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCDRYAKKENDIKCTAED 121
           LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYC+RYAKKEN IKCTAED
Sbjct: 100 LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCERYAKKENGIKCTAED 159

Query: 122 ESEEDEISDNESGSSDDDVAGHADP 147
           ESEEDEIS+++SGSSDDDVAGHADP
Sbjct: 160 ESEEDEISNDDSGSSDDDVAGHADP 184

[Close]
BLAST of HG10008657 vs. ExPASy TrEMBL
Match: A0A6J1E183 (ubiquitin-conjugating enzyme E2-23 kDa-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111429862 PE=3 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 3.6e-71
Identity = 138/146 (94.52%), Postives = 142/146 (97.26%), Query Frame = 0 

Query: 2   KLGLYEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL 61
           K  LYE GVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL
Sbjct: 40  KQSLYETGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL 99

Query: 62  LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCDRYAKKENDIKCTAED 121
           LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYC+R+AKKENDIKCT+ED
Sbjct: 100 LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCERFAKKENDIKCTSED 159

Query: 122 ESEEDEISDNESGSSDDD-VAGHADP 147
           ESEEDE+SDNESGSSDDD VAGHADP
Sbjct: 160 ESEEDEMSDNESGSSDDDNVAGHADP 185

[Close]
BLAST of HG10008657 vs. ExPASy TrEMBL
Match: A0A6J1E228 (ubiquitin-conjugating enzyme E2-23 kDa-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429862 PE=3 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 3.6e-71
Identity = 138/146 (94.52%), Postives = 142/146 (97.26%), Query Frame = 0 

Query: 2   KLGLYEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL 61
           K  LYE GVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL
Sbjct: 61  KQSLYETGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL 120

Query: 62  LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCDRYAKKENDIKCTAED 121
           LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYC+R+AKKENDIKCT+ED
Sbjct: 121 LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCERFAKKENDIKCTSED 180

Query: 122 ESEEDEISDNESGSSDDD-VAGHADP 147
           ESEEDE+SDNESGSSDDD VAGHADP
Sbjct: 181 ESEEDEMSDNESGSSDDDNVAGHADP 206

[Close]
BLAST of HG10008657 vs. ExPASy TrEMBL
Match: A0A0A0LP72 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G005590 PE=3 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 1.0e-70
Identity = 139/149 (93.29%), Postives = 141/149 (94.63%), Query Frame = 0 

Query: 2   KLGLYEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL 61
           K  LYEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL
Sbjct: 40  KESLYEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL 99

Query: 62  LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCDRYAKKENDIKCTAED 121
           LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKD KQYDEKVKEYC+RYAKK+NDIKCT ED
Sbjct: 100 LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDPKQYDEKVKEYCERYAKKDNDIKCTGED 159

Query: 122 ESEEDEISDNESGSS----DDDVAGHADP 147
           ESEEDEISDNESGSS    DDDVAGHADP
Sbjct: 160 ESEEDEISDNESGSSGKDDDDDVAGHADP 188

[Close]
BLAST of HG10008657 vs. TAIR 10
Match: AT5G41340.1 (ubiquitin conjugating enzyme 4 )

HSP 1 Score: 206.5 bits (524), Expect = 1.5e-53
Identity = 103/148 (69.59%), Postives = 124/148 (83.78%), Query Frame = 0 

Query: 2   KLGLYEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL 61
           K  LY+ GVWKIRVELPDAYPYKSPS+GF+ KI+HPNVDELSGSVCLDVINQ+WSPMFDL
Sbjct: 40  KDSLYQGGVWKIRVELPDAYPYKSPSVGFITKIYHPNVDELSGSVCLDVINQTWSPMFDL 99

Query: 62  LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCDRYAK--KENDIKCTA 121
           +NVFE FLPQLLLYPNPSDPLNG+AA+LMM+DR  Y+++VKEYC++YAK  + ++ K + 
Sbjct: 100 VNVFETFLPQLLLYPNPSDPLNGEAAALMMRDRPAYEQRVKEYCEKYAKPGEGSEDKSSD 159

Query: 122 EDESEEDEISDNESGSSDD-DVAGHADP 147
           E+ SEE+  SDNE    DD  +AG  DP
Sbjct: 160 EELSEEEYGSDNEDDDDDDVAIAGKPDP 187

[Close]
BLAST of HG10008657 vs. TAIR 10
Match: AT1G63800.1 (ubiquitin-conjugating enzyme 5 )

HSP 1 Score: 205.3 bits (521), Expect = 3.3e-53
Identity = 101/150 (67.33%), Postives = 122/150 (81.33%), Query Frame = 0 

Query: 2   KLGLYEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDL 61
           K  +YE GVWKIRVELPDAYPYKSPS+GF+ KI+HPNVDE+SGSVCLDVINQ+WSPMFDL
Sbjct: 40  KDSIYEGGVWKIRVELPDAYPYKSPSVGFITKIYHPNVDEMSGSVCLDVINQTWSPMFDL 99

Query: 62  LNVFEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCDRYAKKENDIKCTAED 121
           +NVFE FLPQLLLYPNPSDPLNG+AA+LMM+DR  Y+++VKEYC++YAK   D     E+
Sbjct: 100 VNVFETFLPQLLLYPNPSDPLNGEAAALMMRDRPTYEQRVKEYCEKYAKPRAD----TEE 159

Query: 122 ESEEDEISDNESGSSDDD-----VAGHADP 147
            S +DE+S++E  S  DD     +AG  DP
Sbjct: 160 MSSDDEMSEDEYASDGDDEDDVAIAGKLDP 185

[Close]
BLAST of HG10008657 vs. TAIR 10
Match: AT2G46030.1 (ubiquitin-conjugating enzyme 6 )

HSP 1 Score: 201.1 bits (510), Expect = 6.3e-52
Identity = 96/142 (67.61%), Postives = 119/142 (83.80%), Query Frame = 0 

Query: 5   LYEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDLLNV 64
           LY+ GVWKI+VELP+AYPYKSPS+GFVNKI+HPNVDE SG+VCLDVINQ+WSPMFDL+NV
Sbjct: 43  LYQGGVWKIKVELPEAYPYKSPSVGFVNKIYHPNVDESSGAVCLDVINQTWSPMFDLINV 102

Query: 65  FEVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCDRYAKKENDIKCTAEDESE 124
           FE FLPQLLLYPNPSDP NG+AASL+M+DR  Y+ KVKEYC++YAK E  +    +D+S 
Sbjct: 103 FESFLPQLLLYPNPSDPFNGEAASLLMRDRAAYELKVKEYCEKYAKPEEILSDDDDDDSM 162

Query: 125 EDEISDNESGSSDDDVAGHADP 147
            ++ SD++    DD++ G ADP
Sbjct: 163 SEDGSDSDD-DDDDEIVGKADP 183

[Close]
BLAST of HG10008657 vs. TAIR 10
Match: AT1G64230.1 (ubiquitin-conjugating enzyme 28 )

HSP 1 Score: 86.3 bits (212), Expect = 2.3e-17
Identity = 38/104 (36.54%), Postives = 60/104 (57.69%), Query Frame = 0 

Query: 6   YEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDLLNVF 65
           Y  GV+ + +  P  YP+K P + F  K+FHPNV+  +GS+CLD++ + WSP   +  V 
Sbjct: 45  YSGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNVNS-NGSICLDILKEQWSPALTISKVL 104

Query: 66  EVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCDRYA 110
            + +  LL  PNP DPL  + A +   DR +Y+   + +  +YA
Sbjct: 105 -LSICSLLTDPNPDDPLVPEIAHMYKTDRAKYESTARSWTQKYA 146

[Close]
BLAST of HG10008657 vs. TAIR 10
Match: AT1G64230.2 (ubiquitin-conjugating enzyme 28 )

HSP 1 Score: 86.3 bits (212), Expect = 2.3e-17
Identity = 38/104 (36.54%), Postives = 60/104 (57.69%), Query Frame = 0 

Query: 6   YEAGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDLLNVF 65
           Y  GV+ + +  P  YP+K P + F  K+FHPNV+  +GS+CLD++ + WSP   +  V 
Sbjct: 45  YSGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNVNS-NGSICLDILKEQWSPALTISKVL 104

Query: 66  EVFLPQLLLYPNPSDPLNGDAASLMMKDRKQYDEKVKEYCDRYA 110
            + +  LL  PNP DPL  + A +   DR +Y+   + +  +YA
Sbjct: 105 -LSICSLLTDPNPDDPLVPEIAHMYKTDRAKYESTARSWTQKYA 146

The following BLAST results are available for this feature:
BLAST of HG10008657 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038880248.11.0e-7296.55ubiquitin-conjugating enzyme E2-23 kDa-like [Benincasa hispida][more]
XP_022988415.13.0e-7295.17ubiquitin-conjugating enzyme E2-23 kDa-like [Cucurbita maxima][more]
XP_022135179.12.0e-7195.17ubiquitin-conjugating enzyme E2-23 kDa-like isoform X1 [Momordica charantia] >XP... [more]
XP_022921689.17.4e-7194.52ubiquitin-conjugating enzyme E2-23 kDa-like isoform X2 [Cucurbita moschata] >XP_... [more]
XP_022921686.17.4e-7194.52ubiquitin-conjugating enzyme E2-23 kDa-like isoform X1 [Cucurbita moschata][more]
BLAST of HG10008657 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P165776.6e-5470.55Ubiquitin-conjugating enzyme E2-23 kDa OS=Triticum aestivum OX=4565 GN=UBC4 PE=1... [more]
P427482.1e-5269.59Ubiquitin-conjugating enzyme E2 4 OS=Arabidopsis thaliana OX=3702 GN=UBC4 PE=2 S... [more]
P427494.7e-5267.33Ubiquitin-conjugating enzyme E2 5 OS=Arabidopsis thaliana OX=3702 GN=UBC5 PE=2 S... [more]
P427508.9e-5167.61Ubiquitin-conjugating enzyme E2 6 OS=Arabidopsis thaliana OX=3702 GN=UBC6 PE=2 S... [more]
Q9P7R46.2e-4462.68Ubiquitin-conjugating enzyme E2 8 OS=Schizosaccharomyces pombe (strain 972 / ATC... [more]
BLAST of HG10008657 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1JJH61.5e-7295.17ubiquitin-conjugating enzyme E2-23 kDa-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1C0G29.5e-7295.17ubiquitin-conjugating enzyme E2-23 kDa-like isoform X1 OS=Momordica charantia OX... [more]
A0A6J1E1833.6e-7194.52ubiquitin-conjugating enzyme E2-23 kDa-like isoform X2 OS=Cucurbita moschata OX=... [more]
A0A6J1E2283.6e-7194.52ubiquitin-conjugating enzyme E2-23 kDa-like isoform X1 OS=Cucurbita moschata OX=... [more]
A0A0A0LP721.0e-7093.29UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G005590 PE... [more]
BLAST of HG10008657 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G41340.11.5e-5369.59ubiquitin conjugating enzyme 4 [more]
AT1G63800.13.3e-5367.33ubiquitin-conjugating enzyme 5 [more]
AT2G46030.16.3e-5267.61ubiquitin-conjugating enzyme 6 [more]
AT1G64230.12.3e-1736.54ubiquitin-conjugating enzyme 28 [more]
AT1G64230.22.3e-1736.54ubiquitin-conjugating enzyme 28 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 94..114
NoneNo IPR availableSMARTSM00212ubc_7coord: 1..110
e-value: 9.3E-26
score: 101.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 115..146
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 119..134
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 3..146
NoneNo IPR availablePANTHERPTHR24068:SF332UBIQUITIN-CONJUGATING ENZYME E2-23 KDA-LIKEcoord: 3..146
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 2..124
e-value: 3.2E-40
score: 139.3
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 4..111
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 4..104
e-value: 5.2E-33
score: 113.7
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 1..110
score: 36.532791
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 6..105
e-value: 5.34275E-41
score: 131.941
IPR023313Ubiquitin-conjugating enzyme, active sitePROSITEPS00183UBC_1coord: 35..51

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10008657.1HG10008657.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0061631 ubiquitin conjugating enzyme activity