HG10008550 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10008550
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionSNAP25 homologous protein SNAP33
LocationChr10: 24094750 .. 24097086 (+)
RNA-Seq ExpressionHG10008550
SyntenyHG10008550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCACCTTTTAGGAAATCCCCTATAAATGTTGCTAGGCACGGCACAAGCCAACCTGCTAATCCTGCTCGTTCCAGTCCAAACCCAGTTGATTCAAATGACTTAGACAAGAGGCAAAAACACAAGTCCTCAAGAATCACCACTTCTCATCAAGCAACTGTAGCACCAAATCCCAACTCCAATCTTTTCGACGATCATCTCGAGAAAGAGACTGATTCTACTTCATATTCTTCATCTTCAAGTTCATATTCATGGTCATCAAGAAACAGATACAAGAATGATTTCAGGGACTCAGGTGGGATTGAGAATCAAACAGTGGAAGAGTTGGAGAATTATGCTGTATACAAGGCTGAGGAGACAACTAACTCAGTCAACAACTGCCTGAAGATTGCTGAGGACATGAGAGAAGATGCAACCAAAACCTTAGTTGCCTTGCATCAACAGGGTGAGCAAATCACTCGGACCCATGTGGTTACTGCTGATATCGACAACGATCTTAGTCGGGTACGTGGAACGAATTAACCGCCATATCAATGACATGTTTGAAGTGATTTTGAAATGGTTAAAATCACTTTTGTCTTGTTCAATAATCATTCAAAATTAGTTTCATGTTTGGTTTACACTTTTAAATGTGGTTGTCATCCTATTAAAATTGATTTTAAACGATTAAAAACTTGTAAAAAAAAAACTCTTGAACCTGCCCTTTTCTTTCCTTATGTCCTGAATAAACAAACCTATGCACTAAATATAGGCTTAGCTTCATTTATGGATGCTTTTTGTAAAGATATTGAAATTTATTTTTGGTTATGAAATGATCATCAATATTTTACGATGTTCATTGCAGGGTGAGAAACTTCTTGGAAGCTTAGGAGGAATATTCTCTAAGACTTGGAAGCCAAAGAAAGGTCGTCAGATAACAGGCCCTGTTATCTTTGAAGGTAATATTTTCAAATTTTGGCTGTGGAAGAAAATAATTAAGAAAAATAGAACTGCTTTTTATAACTTCTTTTTTCTTTGTTTTATTATTTAATGATCTAAAAGTTTTACATGAATATATATATTTTTTTAATTAAATATTGATTTTTTATGTTATACATTTTTTAAAAAATAAATAAATAGAACAATACGTTTATTTATGTTTTGGAAAAAAAACTTCAAACTGAAAAAAAAAAAATAGAACAGTTAATAAACACTTATGTTTCTATTCCTTACTCTAACTCGGGATGTACTAGAAAAAAGACTCAATTATGTAACGATAAAACTAAAAAAATACTATTTACCAAAAACATATAATCTATTGTTGAACGGACCTTACCGCAAATGAATATATTTTGTTTTTTCATTCTCAATAATAAATGAAACTTCTAAAAAAAATTATAGTGAAAATGTTTGGACAAATAAGACTCGTGCTATCTTTTTTAATACTAATTACCCATAAATTGAAGATAAAATTTTTTTAATACTAATTACCCATAAATTGAAGATAAAATTGAGTCAGAAGATCTAAAAACAAGAAACATAAACATTATCTCCGTAATTTTAAATTATTTGAACTAATTTGAACAAACTTAATCTATAGAACCAAAAAGGTTTCAACTTTCCCACTAATCAGATGGATTAAGTTGATGGCCAATTAAGTTTTTAAAAATATTATTAAATTTTTAATAAATAAATTTGATTTGCCTTGGTTGCAGGGGATCAGGCTGTTATAAGCAAGGGCAACCATTTGGAGCAAAGGGAGAAGTTGGGGTTAAGTCGGGCACCAAATCCACGGTCCTCACGTGCCCCACATCCTGAGCCAACCAACTCACTTCAGAAAGTTGAGGTTTGTAGTTATTCAGTTATAGTGCTTCTCATGGAAGTACTTACAGAAAAAGCACTTCACTTACAATACACAAACGTGAAGGGAGCAGAATAGAAGAATATTTGAAAACACTTAACAAGAAATTTGTTCTAAACCAGGTGGAAAAGTTCAAGCAAGAGGATGCTCTTTCAGATTTGAGCAATATATTGGGAGAGCTTAAAGAAATGGCTGTTGATATGGGATCAGAACTAGACAGGTACCTTTTTTTTTTCTTCTTAGAATCACTTCTCAAATTGCCCAGGAGAAATAGAAAAAATATGAATGAGAAATAAAAAAAAACTGAGAACGTTACCAAATGGGTAAAAATGCCAAGTTTTCTTTCTGTTTCTTTTATTTTTGTTAAACTCAGCTGGAATCAATCTCATTATTTCAGGCAAAATAAGGCTTTGGATAACTTGTTTGATGATGCCAATGAGGTCACGATTCGTGTCGATAATGCTAACCAGCGTGCTCGTCATCTGCTTAGAAAGTAG

mRNA sequence

ATGTCACCTTTTAGGAAATCCCCTATAAATGTTGCTAGGCACGGCACAAGCCAACCTGCTAATCCTGCTCGTTCCAGTCCAAACCCAGTTGATTCAAATGACTTAGACAAGAGGCAAAAACACAAGTCCTCAAGAATCACCACTTCTCATCAAGCAACTGTAGCACCAAATCCCAACTCCAATCTTTTCGACGATCATCTCGAGAAAGAGACTGATTCTACTTCATATTCTTCATCTTCAAGTTCATATTCATGGTCATCAAGAAACAGATACAAGAATGATTTCAGGGACTCAGGTGGGATTGAGAATCAAACAGTGGAAGAGTTGGAGAATTATGCTGTATACAAGGCTGAGGAGACAACTAACTCAGTCAACAACTGCCTGAAGATTGCTGAGGACATGAGAGAAGATGCAACCAAAACCTTAGTTGCCTTGCATCAACAGGGTGAGCAAATCACTCGGACCCATGTGGTTACTGCTGATATCGACAACGATCTTAGTCGGGGTGAGAAACTTCTTGGAAGCTTAGGAGGAATATTCTCTAAGACTTGGAAGCCAAAGAAAGGTCGTCAGATAACAGGCCCTGTTATCTTTGAAGGGGATCAGGCTGTTATAAGCAAGGGCAACCATTTGGAGCAAAGGGAGAAGTTGGGGTTAAGTCGGGCACCAAATCCACGGTCCTCACGTGCCCCACATCCTGAGCCAACCAACTCACTTCAGAAAGTTGAGGTGGAAAAGTTCAAGCAAGAGGATGCTCTTTCAGATTTGAGCAATATATTGGGAGAGCTTAAAGAAATGGCTGTTGATATGGGATCAGAACTAGACAGGCAAAATAAGGCTTTGGATAACTTGTTTGATGATGCCAATGAGGTCACGATTCGTGTCGATAATGCTAACCAGCGTGCTCGTCATCTGCTTAGAAAGTAG

Coding sequence (CDS)

ATGTCACCTTTTAGGAAATCCCCTATAAATGTTGCTAGGCACGGCACAAGCCAACCTGCTAATCCTGCTCGTTCCAGTCCAAACCCAGTTGATTCAAATGACTTAGACAAGAGGCAAAAACACAAGTCCTCAAGAATCACCACTTCTCATCAAGCAACTGTAGCACCAAATCCCAACTCCAATCTTTTCGACGATCATCTCGAGAAAGAGACTGATTCTACTTCATATTCTTCATCTTCAAGTTCATATTCATGGTCATCAAGAAACAGATACAAGAATGATTTCAGGGACTCAGGTGGGATTGAGAATCAAACAGTGGAAGAGTTGGAGAATTATGCTGTATACAAGGCTGAGGAGACAACTAACTCAGTCAACAACTGCCTGAAGATTGCTGAGGACATGAGAGAAGATGCAACCAAAACCTTAGTTGCCTTGCATCAACAGGGTGAGCAAATCACTCGGACCCATGTGGTTACTGCTGATATCGACAACGATCTTAGTCGGGGTGAGAAACTTCTTGGAAGCTTAGGAGGAATATTCTCTAAGACTTGGAAGCCAAAGAAAGGTCGTCAGATAACAGGCCCTGTTATCTTTGAAGGGGATCAGGCTGTTATAAGCAAGGGCAACCATTTGGAGCAAAGGGAGAAGTTGGGGTTAAGTCGGGCACCAAATCCACGGTCCTCACGTGCCCCACATCCTGAGCCAACCAACTCACTTCAGAAAGTTGAGGTGGAAAAGTTCAAGCAAGAGGATGCTCTTTCAGATTTGAGCAATATATTGGGAGAGCTTAAAGAAATGGCTGTTGATATGGGATCAGAACTAGACAGGCAAAATAAGGCTTTGGATAACTTGTTTGATGATGCCAATGAGGTCACGATTCGTGTCGATAATGCTAACCAGCGTGCTCGTCATCTGCTTAGAAAGTAG

Protein sequence

MSPFRKSPINVARHGTSQPANPARSSPNPVDSNDLDKRQKHKSSRITTSHQATVAPNPNSNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEETTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGIFSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNANQRARHLLRK
Homology
BLAST of HG10008550 vs. NCBI nr
Match: XP_038879751.1 (SNAP25 homologous protein SNAP33-like [Benincasa hispida] >XP_038879752.1 SNAP25 homologous protein SNAP33-like [Benincasa hispida] >XP_038879753.1 SNAP25 homologous protein SNAP33-like [Benincasa hispida])

HSP 1 Score: 560.8 bits (1444), Expect = 7.1e-156
Identity = 292/309 (94.50%), Postives = 303/309 (98.06%), Query Frame = 0

Query: 1   MSPFRKSPINVARHGTSQPANPARSSPNPVDSND-LDKRQKHKSSRITTSHQATVAPNPN 60
           MSPFRKSPINVARHG+SQP N AR SPNPVDSND LDK+QK+KSSRIT+SHQATVAPNP+
Sbjct: 1   MSPFRKSPINVARHGSSQPDNSARPSPNPVDSNDNLDKKQKYKSSRITSSHQATVAPNPS 60

Query: 61  SNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           SNLFDDHLE+ET+S SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE
Sbjct: 61  SNLFDDHLEQETNSASYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120

Query: 121 TTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TTN+VNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI
Sbjct: 121 TTNAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRS RAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSLRAPHPEPTNSL 240

Query: 241 QKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNAN 300
           +KVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALD+LFDDA+EVTIRVDNAN
Sbjct: 241 KKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDHLFDDADEVTIRVDNAN 300

Query: 301 QRARHLLRK 309
           QRARHLLRK
Sbjct: 301 QRARHLLRK 309

BLAST of HG10008550 vs. NCBI nr
Match: XP_004138184.1 (SNAP25 homologous protein SNAP33 [Cucumis sativus] >KGN63709.1 hypothetical protein Csa_013922 [Cucumis sativus])

HSP 1 Score: 547.7 bits (1410), Expect = 6.2e-152
Identity = 288/309 (93.20%), Postives = 299/309 (96.76%), Query Frame = 0

Query: 1   MSPFRKSPINVARHGTSQPANPARSSPNPVDSND-LDKRQKHKSSRITTSHQATVAPNPN 60
           MSPFRKSPINV RHG+SQPAN ARSSPNPV+SND LDK+QK +SSRIT+SHQAT APNP 
Sbjct: 1   MSPFRKSPINVGRHGSSQPANSARSSPNPVNSNDNLDKKQK-QSSRITSSHQATAAPNPK 60

Query: 61  SNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           S LFDDHLEK TDS+SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELE+YAVYKAEE
Sbjct: 61  SALFDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAEE 120

Query: 121 TTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TTNSVNNCLKIAED+REDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI
Sbjct: 121 TTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGD AVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 QKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNAN 300
           QKVEVEK KQ+DALSDLSNILGELKEMAVDMGSELD+QNKALD+LFDDANEVTIRVDNAN
Sbjct: 241 QKVEVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDNAN 300

Query: 301 QRARHLLRK 309
           QRARHLLRK
Sbjct: 301 QRARHLLRK 308

BLAST of HG10008550 vs. NCBI nr
Match: XP_008453247.1 (PREDICTED: putative SNAP25 homologous protein SNAP30 [Cucumis melo] >KAA0057978.1 putative SNAP25-like proteinous protein SNAP30 [Cucumis melo var. makuwa])

HSP 1 Score: 540.4 bits (1391), Expect = 9.9e-150
Identity = 287/309 (92.88%), Postives = 296/309 (95.79%), Query Frame = 0

Query: 1   MSPFRKSPINVARHGTSQPANPARSSPNPVDSND-LDKRQKHKSSRITTSHQATVAPNPN 60
           MSPFRKSPINV RH ++QPANPARSSPN V+SND LDKRQK +SSRIT+SHQ T APNP 
Sbjct: 1   MSPFRKSPINVVRHSSNQPANPARSSPNLVNSNDNLDKRQK-QSSRITSSHQETAAPNPK 60

Query: 61  SNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           S LFDDHLEK TDS SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE
Sbjct: 61  SALFDDHLEKGTDS-SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120

Query: 121 TTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TTNSVNNCLKIAED+REDATKTLVALHQQGEQITRTH+VTADIDNDLSRGEKLLGSLGGI
Sbjct: 121 TTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHMVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGD AVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 QKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNAN 300
           QKVEVEK KQEDALSDLSNILGELKEMAVDMGSELD+QN+ALDNLFDDANEVTIRVDNAN
Sbjct: 241 QKVEVEKVKQEDALSDLSNILGELKEMAVDMGSELDKQNRALDNLFDDANEVTIRVDNAN 300

Query: 301 QRARHLLRK 309
           QRARHLLRK
Sbjct: 301 QRARHLLRK 307

BLAST of HG10008550 vs. NCBI nr
Match: XP_022921394.1 (putative SNAP25 homologous protein SNAP30 [Cucurbita moschata] >XP_022921396.1 putative SNAP25 homologous protein SNAP30 [Cucurbita moschata] >XP_022921397.1 putative SNAP25 homologous protein SNAP30 [Cucurbita moschata])

HSP 1 Score: 533.5 bits (1373), Expect = 1.2e-147
Identity = 277/309 (89.64%), Postives = 293/309 (94.82%), Query Frame = 0

Query: 1   MSPFRKSPINVARHGTSQPANPARSSPNPVDSND-LDKRQKHKSSRITTSHQATVAPNPN 60
           MS FRKSPI  ARHG+SQPANPARS+  P+DSND LDKRQKHKSSRIT+SHQATVAPNP+
Sbjct: 1   MSHFRKSPIRFARHGSSQPANPARSTSQPIDSNDNLDKRQKHKSSRITSSHQATVAPNPS 60

Query: 61  SNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           SNLFDDHLEKETD    SSS SSYSWSSRNRYKNDFRDSGG+++QTVEELENYA YKAEE
Sbjct: 61  SNLFDDHLEKETD----SSSHSSYSWSSRNRYKNDFRDSGGLQSQTVEELENYAAYKAEE 120

Query: 121 TTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TTN+VNNCLKIAEDMREDAT TLVALH QGEQITRTH+VTADIDNDLSRGEKLLGSLGGI
Sbjct: 121 TTNAVNNCLKIAEDMREDATNTLVALHHQGEQITRTHMVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQRE+LGLSRAPNPRSSRAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQRERLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 QKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNAN 300
           QKVEVEK KQEDALSDLSNILGELK MAVDMG+ELDRQNKALDNLFDDA+E+T+RVDNAN
Sbjct: 241 QKVEVEKVKQEDALSDLSNILGELKGMAVDMGTELDRQNKALDNLFDDADEITVRVDNAN 300

Query: 301 QRARHLLRK 309
           +RARHLLRK
Sbjct: 301 RRARHLLRK 305

BLAST of HG10008550 vs. NCBI nr
Match: XP_023516514.1 (putative SNAP25 homologous protein SNAP30 [Cucurbita pepo subsp. pepo] >XP_023516515.1 putative SNAP25 homologous protein SNAP30 [Cucurbita pepo subsp. pepo] >XP_023516516.1 putative SNAP25 homologous protein SNAP30 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 532.3 bits (1370), Expect = 2.7e-147
Identity = 276/309 (89.32%), Postives = 292/309 (94.50%), Query Frame = 0

Query: 1   MSPFRKSPINVARHGTSQPANPARSSPNPVDSND-LDKRQKHKSSRITTSHQATVAPNPN 60
           MS FRKSPI  ARHG+SQPANPARS   P+DSND LDKRQKHKSSRIT+SHQATVAPNP+
Sbjct: 1   MSHFRKSPIRFARHGSSQPANPARSPSQPIDSNDNLDKRQKHKSSRITSSHQATVAPNPS 60

Query: 61  SNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           SNLFDDHLEKETD    SSS SSYSWSSRNRYKNDFRDSGG+++QTVEELENYA YKAEE
Sbjct: 61  SNLFDDHLEKETD----SSSHSSYSWSSRNRYKNDFRDSGGLQSQTVEELENYAAYKAEE 120

Query: 121 TTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TTN+VNNCLKIAEDMREDAT TLVALH QGEQITRTH+VTADIDNDLSRGEKLLGSLGGI
Sbjct: 121 TTNAVNNCLKIAEDMREDATNTLVALHHQGEQITRTHMVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGP+IFEGDQAVISKGNHLEQRE+LGLSRAPNPRSSRAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPIIFEGDQAVISKGNHLEQRERLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 QKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNAN 300
           QKVEVEK KQEDALSDLSNILGELK MAVDMG+ELDRQNKALDNLFDDA+E+T+RVDNAN
Sbjct: 241 QKVEVEKVKQEDALSDLSNILGELKGMAVDMGTELDRQNKALDNLFDDADEITVRVDNAN 300

Query: 301 QRARHLLRK 309
           +RARHLLRK
Sbjct: 301 RRARHLLRK 305

BLAST of HG10008550 vs. ExPASy Swiss-Prot
Match: Q9S7P9 (SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 2.1e-78
Identity = 174/310 (56.13%), Postives = 217/310 (70.00%), Query Frame = 0

Query: 1   MSPFRKSPINVARHGTSQPANPARSSPNPVDSND-LDKRQKHKSSRITTSHQATVAPNPN 60
           M   RKSP N+ +H +    +   S PNP DS+D  D +     S+ TTS  +       
Sbjct: 1   MFGLRKSPANLPKHNS---VDLKSSKPNPFDSDDESDNKHTLNPSKRTTSEPSLA---DM 60

Query: 61  SNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           +N F     ++ DS   SSS  S   +S+ +YKN+FRDSGGIENQ+V+ELE YAVYKAEE
Sbjct: 61  TNPFGGERVQKGDS---SSSKQSLFSNSKYQYKNNFRDSGGIENQSVQELEGYAVYKAEE 120

Query: 121 TTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TT SV  CLK+AED+R DAT+TLV LH QGEQITRTH    +ID+DLSRGEKLLGSLGG+
Sbjct: 121 TTKSVQGCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGGM 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRS-SRAPHPEPTNS 240
           FSKTWKPKK R I GPV+   D +   + NHLE+REKLGL+ AP  +S +R P PE  ++
Sbjct: 181 FSKTWKPKKTRPINGPVVTR-DDSPTRRVNHLEKREKLGLNSAPRGQSRTREPLPESADA 240

Query: 241 LQKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNA 300
            Q+VE+EK KQ+D LSDLS+ILGELK MAVDMGSE+++QNK LD+L DD +E+  RV  +
Sbjct: 241 YQRVEMEKAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQS 300

Query: 301 NQRARHLLRK 309
           NQR R LL K
Sbjct: 301 NQRGRRLLGK 300

BLAST of HG10008550 vs. ExPASy Swiss-Prot
Match: Q9LMG8 (Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNAP30 PE=3 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 5.6e-71
Identity = 143/220 (65.00%), Postives = 169/220 (76.82%), Query Frame = 0

Query: 89  NRYKNDFRDSGGIENQTVEELENYAVYKAEETTNSVNNCLKIAEDMREDATKTLVALHQQ 148
           ++YKN F DSGG+++QT EELE YAVYKAEETT  VNNCLKIAED+R D  +TL  LHQQ
Sbjct: 46  DKYKNGFNDSGGLQSQTTEELEKYAVYKAEETTKGVNNCLKIAEDIRSDGARTLEMLHQQ 105

Query: 149 GEQITRTHVVTADIDNDLSRGEKLLGSLGGIFSKTWKPKKGRQITGPVIFEGDQAVISKG 208
           GEQI RTH +  D+D DLSRGEKLL +LGG+FSK WKPKK + ITGP+I   D+      
Sbjct: 106 GEQINRTHEMAVDMDKDLSRGEKLLNNLGGMFSKPWKPKKTKNITGPMI-TPDKPSKKSE 165

Query: 209 NHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQKVEVEKFKQEDALSDLSNILGELKEMAV 268
           NH E+REKLGL  A    SS+    +PTN+LQKVE EK KQ+D LSDLS+ILG+LK MAV
Sbjct: 166 NHKEEREKLGLG-AKGRSSSQPALDQPTNALQKVEQEKAKQDDGLSDLSDILGDLKSMAV 225

Query: 269 DMGSELDRQNKALDNLFDDANEVTIRVDNANQRARHLLRK 309
           DMGSE+D+QNKALD+L DD +E+  RV  ANQRARHLL K
Sbjct: 226 DMGSEIDKQNKALDHLGDDVDELNSRVQGANQRARHLLSK 263

BLAST of HG10008550 vs. ExPASy Swiss-Prot
Match: Q9SD96 (SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 4.2e-58
Identity = 130/252 (51.59%), Postives = 173/252 (68.65%), Query Frame = 0

Query: 57  NPNSNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYK 116
           N + N FDD  E     +               R+ +  + SGG ENQTV+ELE+YAVY 
Sbjct: 5   NSSWNPFDDEKEAAKSFSLNPFDDDDDDKEVEKRFTSSLKPSGGKENQTVQELESYAVYN 64

Query: 117 AEETTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSL 176
           +EETT +V  CLK+AE++R DA+KTLV L++QG+QITRTH  T D+D+ LSRGEK+LG L
Sbjct: 65  SEETTKTVQGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSRGEKILGRL 124

Query: 177 GGIFSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPT 236
           GG+FS+TWKPKK R ITGPVI +GD     K   L+ REKLGL+ +  P+S     PE  
Sbjct: 125 GGVFSRTWKPKKSRSITGPVITKGDSPK-RKVIDLKTREKLGLNPSLKPKSKTL--PEAV 184

Query: 237 NSLQKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVD 296
           ++ QK ++   KQ++AL+DLS +LGELK MAVDMG+ ++RQ   LD+L D+A+E+  RV 
Sbjct: 185 DAYQKTQIA--KQDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNADELNYRVK 244

Query: 297 NANQRARHLLRK 309
            +NQRAR+LLRK
Sbjct: 245 QSNQRARYLLRK 251

BLAST of HG10008550 vs. ExPASy Swiss-Prot
Match: Q17QQ3 (Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 4.8e-14
Identity = 61/213 (28.64%), Postives = 102/213 (47.89%), Query Frame = 0

Query: 106 VEELENYAVYKAEETTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDND 165
           +EE++  A   A+E+  S    L++ E+ ++   +TLV L +QGEQ+ R       I+ D
Sbjct: 11  LEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 166 LSRGEKLLGSLGGI------------FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQ 225
           +   EK L  LG               S  +K   G    G V  +  + V       ++
Sbjct: 71  MKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVV-------DE 130

Query: 226 REKLGLSRAPNPRSSRAPHPEPTNSLQKVEVEKFKQEDALSDLSNILGELKEMAVDMGSE 285
           RE++ +S     R         TN  ++ E+     ++ L  +S I+G L+ MA+DMG+E
Sbjct: 131 REQMAISGGFIRR--------VTNDARENEM-----DENLEQVSGIIGNLRHMALDMGNE 190

Query: 286 LDRQNKALDNLFDDANEVTIRVDNANQRARHLL 307
           +D QN+ +D + + A+    R+D ANQRA  +L
Sbjct: 191 IDTQNRQIDRIMEKADSNKTRIDEANQRATKML 203

BLAST of HG10008550 vs. ExPASy Swiss-Prot
Match: P60878 (Synaptosomal-associated protein 25 OS=Gallus gallus OX=9031 GN=SNAP25 PE=1 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 4.8e-14
Identity = 61/213 (28.64%), Postives = 102/213 (47.89%), Query Frame = 0

Query: 106 VEELENYAVYKAEETTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDND 165
           +EE++  A   A+E+  S    L++ E+ ++   +TLV L +QGEQ+ R       I+ D
Sbjct: 11  LEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 166 LSRGEKLLGSLGGI------------FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQ 225
           +   EK L  LG               S  +K   G    G V  +  + V       ++
Sbjct: 71  MKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVV-------DE 130

Query: 226 REKLGLSRAPNPRSSRAPHPEPTNSLQKVEVEKFKQEDALSDLSNILGELKEMAVDMGSE 285
           RE++ +S     R         TN  ++ E+     ++ L  +S I+G L+ MA+DMG+E
Sbjct: 131 REQMAISGGFIRR--------VTNDARENEM-----DENLEQVSGIIGNLRHMALDMGNE 190

Query: 286 LDRQNKALDNLFDDANEVTIRVDNANQRARHLL 307
           +D QN+ +D + + A+    R+D ANQRA  +L
Sbjct: 191 IDTQNRQIDRIMEKADSNKTRIDEANQRATKML 203

BLAST of HG10008550 vs. ExPASy TrEMBL
Match: A0A0A0LRS5 (t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G012100 PE=4 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 3.0e-152
Identity = 288/309 (93.20%), Postives = 299/309 (96.76%), Query Frame = 0

Query: 1   MSPFRKSPINVARHGTSQPANPARSSPNPVDSND-LDKRQKHKSSRITTSHQATVAPNPN 60
           MSPFRKSPINV RHG+SQPAN ARSSPNPV+SND LDK+QK +SSRIT+SHQAT APNP 
Sbjct: 1   MSPFRKSPINVGRHGSSQPANSARSSPNPVNSNDNLDKKQK-QSSRITSSHQATAAPNPK 60

Query: 61  SNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           S LFDDHLEK TDS+SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELE+YAVYKAEE
Sbjct: 61  SALFDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAEE 120

Query: 121 TTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TTNSVNNCLKIAED+REDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI
Sbjct: 121 TTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGD AVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 QKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNAN 300
           QKVEVEK KQ+DALSDLSNILGELKEMAVDMGSELD+QNKALD+LFDDANEVTIRVDNAN
Sbjct: 241 QKVEVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDNAN 300

Query: 301 QRARHLLRK 309
           QRARHLLRK
Sbjct: 301 QRARHLLRK 308

BLAST of HG10008550 vs. ExPASy TrEMBL
Match: A0A5A7US66 (Putative SNAP25-like proteinous protein SNAP30 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002820 PE=4 SV=1)

HSP 1 Score: 540.4 bits (1391), Expect = 4.8e-150
Identity = 287/309 (92.88%), Postives = 296/309 (95.79%), Query Frame = 0

Query: 1   MSPFRKSPINVARHGTSQPANPARSSPNPVDSND-LDKRQKHKSSRITTSHQATVAPNPN 60
           MSPFRKSPINV RH ++QPANPARSSPN V+SND LDKRQK +SSRIT+SHQ T APNP 
Sbjct: 1   MSPFRKSPINVVRHSSNQPANPARSSPNLVNSNDNLDKRQK-QSSRITSSHQETAAPNPK 60

Query: 61  SNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           S LFDDHLEK TDS SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE
Sbjct: 61  SALFDDHLEKGTDS-SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120

Query: 121 TTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TTNSVNNCLKIAED+REDATKTLVALHQQGEQITRTH+VTADIDNDLSRGEKLLGSLGGI
Sbjct: 121 TTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHMVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGD AVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 QKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNAN 300
           QKVEVEK KQEDALSDLSNILGELKEMAVDMGSELD+QN+ALDNLFDDANEVTIRVDNAN
Sbjct: 241 QKVEVEKVKQEDALSDLSNILGELKEMAVDMGSELDKQNRALDNLFDDANEVTIRVDNAN 300

Query: 301 QRARHLLRK 309
           QRARHLLRK
Sbjct: 301 QRARHLLRK 307

BLAST of HG10008550 vs. ExPASy TrEMBL
Match: A0A1S3BVT2 (putative SNAP25 homologous protein SNAP30 OS=Cucumis melo OX=3656 GN=LOC103494024 PE=4 SV=1)

HSP 1 Score: 540.4 bits (1391), Expect = 4.8e-150
Identity = 287/309 (92.88%), Postives = 296/309 (95.79%), Query Frame = 0

Query: 1   MSPFRKSPINVARHGTSQPANPARSSPNPVDSND-LDKRQKHKSSRITTSHQATVAPNPN 60
           MSPFRKSPINV RH ++QPANPARSSPN V+SND LDKRQK +SSRIT+SHQ T APNP 
Sbjct: 1   MSPFRKSPINVVRHSSNQPANPARSSPNLVNSNDNLDKRQK-QSSRITSSHQETAAPNPK 60

Query: 61  SNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           S LFDDHLEK TDS SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE
Sbjct: 61  SALFDDHLEKGTDS-SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120

Query: 121 TTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TTNSVNNCLKIAED+REDATKTLVALHQQGEQITRTH+VTADIDNDLSRGEKLLGSLGGI
Sbjct: 121 TTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHMVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGD AVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 QKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNAN 300
           QKVEVEK KQEDALSDLSNILGELKEMAVDMGSELD+QN+ALDNLFDDANEVTIRVDNAN
Sbjct: 241 QKVEVEKVKQEDALSDLSNILGELKEMAVDMGSELDKQNRALDNLFDDANEVTIRVDNAN 300

Query: 301 QRARHLLRK 309
           QRARHLLRK
Sbjct: 301 QRARHLLRK 307

BLAST of HG10008550 vs. ExPASy TrEMBL
Match: A0A6J1E197 (putative SNAP25 homologous protein SNAP30 OS=Cucurbita moschata OX=3662 GN=LOC111429687 PE=4 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 5.9e-148
Identity = 277/309 (89.64%), Postives = 293/309 (94.82%), Query Frame = 0

Query: 1   MSPFRKSPINVARHGTSQPANPARSSPNPVDSND-LDKRQKHKSSRITTSHQATVAPNPN 60
           MS FRKSPI  ARHG+SQPANPARS+  P+DSND LDKRQKHKSSRIT+SHQATVAPNP+
Sbjct: 1   MSHFRKSPIRFARHGSSQPANPARSTSQPIDSNDNLDKRQKHKSSRITSSHQATVAPNPS 60

Query: 61  SNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           SNLFDDHLEKETD    SSS SSYSWSSRNRYKNDFRDSGG+++QTVEELENYA YKAEE
Sbjct: 61  SNLFDDHLEKETD----SSSHSSYSWSSRNRYKNDFRDSGGLQSQTVEELENYAAYKAEE 120

Query: 121 TTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TTN+VNNCLKIAEDMREDAT TLVALH QGEQITRTH+VTADIDNDLSRGEKLLGSLGGI
Sbjct: 121 TTNAVNNCLKIAEDMREDATNTLVALHHQGEQITRTHMVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQRE+LGLSRAPNPRSSRAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQRERLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 QKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNAN 300
           QKVEVEK KQEDALSDLSNILGELK MAVDMG+ELDRQNKALDNLFDDA+E+T+RVDNAN
Sbjct: 241 QKVEVEKVKQEDALSDLSNILGELKGMAVDMGTELDRQNKALDNLFDDADEITVRVDNAN 300

Query: 301 QRARHLLRK 309
           +RARHLLRK
Sbjct: 301 RRARHLLRK 305

BLAST of HG10008550 vs. ExPASy TrEMBL
Match: A0A6J1JJU0 (putative SNAP25 homologous protein SNAP30 OS=Cucurbita maxima OX=3661 GN=LOC111485094 PE=4 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 7.2e-146
Identity = 275/309 (89.00%), Postives = 291/309 (94.17%), Query Frame = 0

Query: 1   MSPFRKSPINVARHGTSQPANPARSSPNPVDSND-LDKRQKHKSSRITTSHQATVAPNPN 60
           MS FRKSPI  ARHG+SQPA PARS+  P+DSND  DKRQKHKSSRIT+SHQATVAPNP 
Sbjct: 1   MSHFRKSPIRFARHGSSQPAYPARSTSQPIDSNDNSDKRQKHKSSRITSSHQATVAPNPI 60

Query: 61  SNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           SNLFDDHLEKETD    SSS SSYSWSSRNRYKNDFRDSGG+++QTVEELENYA YKAEE
Sbjct: 61  SNLFDDHLEKETD----SSSHSSYSWSSRNRYKNDFRDSGGLQSQTVEELENYAAYKAEE 120

Query: 121 TTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TTN+VNNCLKIAEDMREDATKTLVALH QGEQITRTH+VTADIDNDLSRGEKLLGSLGGI
Sbjct: 121 TTNAVNNCLKIAEDMREDATKTLVALHHQGEQITRTHMVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQRE+LGLSRAPNPRSSRAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQRERLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 QKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNAN 300
           QKVEVEK KQEDALSDLSNILGELK MAVDMG+ELDRQNKALD+LFDDA+E+T+RVDNAN
Sbjct: 241 QKVEVEKVKQEDALSDLSNILGELKGMAVDMGTELDRQNKALDHLFDDADEITVRVDNAN 300

Query: 301 QRARHLLRK 309
           +RARHLLRK
Sbjct: 301 RRARHLLRK 305

BLAST of HG10008550 vs. TAIR 10
Match: AT5G61210.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 33 )

HSP 1 Score: 293.9 bits (751), Expect = 1.5e-79
Identity = 174/310 (56.13%), Postives = 217/310 (70.00%), Query Frame = 0

Query: 1   MSPFRKSPINVARHGTSQPANPARSSPNPVDSND-LDKRQKHKSSRITTSHQATVAPNPN 60
           M   RKSP N+ +H +    +   S PNP DS+D  D +     S+ TTS  +       
Sbjct: 1   MFGLRKSPANLPKHNS---VDLKSSKPNPFDSDDESDNKHTLNPSKRTTSEPSLA---DM 60

Query: 61  SNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           +N F     ++ DS   SSS  S   +S+ +YKN+FRDSGGIENQ+V+ELE YAVYKAEE
Sbjct: 61  TNPFGGERVQKGDS---SSSKQSLFSNSKYQYKNNFRDSGGIENQSVQELEGYAVYKAEE 120

Query: 121 TTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TT SV  CLK+AED+R DAT+TLV LH QGEQITRTH    +ID+DLSRGEKLLGSLGG+
Sbjct: 121 TTKSVQGCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGGM 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRS-SRAPHPEPTNS 240
           FSKTWKPKK R I GPV+   D +   + NHLE+REKLGL+ AP  +S +R P PE  ++
Sbjct: 181 FSKTWKPKKTRPINGPVVTR-DDSPTRRVNHLEKREKLGLNSAPRGQSRTREPLPESADA 240

Query: 241 LQKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVDNA 300
            Q+VE+EK KQ+D LSDLS+ILGELK MAVDMGSE+++QNK LD+L DD +E+  RV  +
Sbjct: 241 YQRVEMEKAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQS 300

Query: 301 NQRARHLLRK 309
           NQR R LL K
Sbjct: 301 NQRGRRLLGK 300

BLAST of HG10008550 vs. TAIR 10
Match: AT1G13890.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 30 )

HSP 1 Score: 269.2 bits (687), Expect = 4.0e-72
Identity = 143/220 (65.00%), Postives = 169/220 (76.82%), Query Frame = 0

Query: 89  NRYKNDFRDSGGIENQTVEELENYAVYKAEETTNSVNNCLKIAEDMREDATKTLVALHQQ 148
           ++YKN F DSGG+++QT EELE YAVYKAEETT  VNNCLKIAED+R D  +TL  LHQQ
Sbjct: 46  DKYKNGFNDSGGLQSQTTEELEKYAVYKAEETTKGVNNCLKIAEDIRSDGARTLEMLHQQ 105

Query: 149 GEQITRTHVVTADIDNDLSRGEKLLGSLGGIFSKTWKPKKGRQITGPVIFEGDQAVISKG 208
           GEQI RTH +  D+D DLSRGEKLL +LGG+FSK WKPKK + ITGP+I   D+      
Sbjct: 106 GEQINRTHEMAVDMDKDLSRGEKLLNNLGGMFSKPWKPKKTKNITGPMI-TPDKPSKKSE 165

Query: 209 NHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQKVEVEKFKQEDALSDLSNILGELKEMAV 268
           NH E+REKLGL  A    SS+    +PTN+LQKVE EK KQ+D LSDLS+ILG+LK MAV
Sbjct: 166 NHKEEREKLGLG-AKGRSSSQPALDQPTNALQKVEQEKAKQDDGLSDLSDILGDLKSMAV 225

Query: 269 DMGSELDRQNKALDNLFDDANEVTIRVDNANQRARHLLRK 309
           DMGSE+D+QNKALD+L DD +E+  RV  ANQRARHLL K
Sbjct: 226 DMGSEIDKQNKALDHLGDDVDELNSRVQGANQRARHLLSK 263

BLAST of HG10008550 vs. TAIR 10
Match: AT5G07880.1 (synaptosomal-associated protein SNAP25-like 29 )

HSP 1 Score: 226.5 bits (576), Expect = 3.0e-59
Identity = 130/252 (51.59%), Postives = 173/252 (68.65%), Query Frame = 0

Query: 57  NPNSNLFDDHLEKETDSTSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYK 116
           N + N FDD  E     +               R+ +  + SGG ENQTV+ELE+YAVY 
Sbjct: 5   NSSWNPFDDEKEAAKSFSLNPFDDDDDDKEVEKRFTSSLKPSGGKENQTVQELESYAVYN 64

Query: 117 AEETTNSVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSL 176
           +EETT +V  CLK+AE++R DA+KTLV L++QG+QITRTH  T D+D+ LSRGEK+LG L
Sbjct: 65  SEETTKTVQGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSRGEKILGRL 124

Query: 177 GGIFSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPT 236
           GG+FS+TWKPKK R ITGPVI +GD     K   L+ REKLGL+ +  P+S     PE  
Sbjct: 125 GGVFSRTWKPKKSRSITGPVITKGDSPK-RKVIDLKTREKLGLNPSLKPKSKTL--PEAV 184

Query: 237 NSLQKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTIRVD 296
           ++ QK ++   KQ++AL+DLS +LGELK MAVDMG+ ++RQ   LD+L D+A+E+  RV 
Sbjct: 185 DAYQKTQIA--KQDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNADELNYRVK 244

Query: 297 NANQRARHLLRK 309
            +NQRAR+LLRK
Sbjct: 245 QSNQRARYLLRK 251

BLAST of HG10008550 vs. TAIR 10
Match: AT3G09740.1 (syntaxin of plants 71 )

HSP 1 Score: 42.7 bits (99), Expect = 6.1e-04
Identity = 26/70 (37.14%), Postives = 35/70 (50.00%), Query Frame = 0

Query: 234 EPTNSLQKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDNLFDDANEVTI 293
           E +   Q+ E+ K KQE  L  +S  L  LK MA DM  ELDRQ   +D +    +  T 
Sbjct: 163 ESSQFRQEYEMRKIKQEQGLDMISEGLDALKNMASDMNEELDRQVPLMDEIDTKVDRATS 222

Query: 294 RVDNANQRAR 304
            + N N R +
Sbjct: 223 DLKNTNVRLK 232

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038879751.17.1e-15694.50SNAP25 homologous protein SNAP33-like [Benincasa hispida] >XP_038879752.1 SNAP25... [more]
XP_004138184.16.2e-15293.20SNAP25 homologous protein SNAP33 [Cucumis sativus] >KGN63709.1 hypothetical prot... [more]
XP_008453247.19.9e-15092.88PREDICTED: putative SNAP25 homologous protein SNAP30 [Cucumis melo] >KAA0057978.... [more]
XP_022921394.11.2e-14789.64putative SNAP25 homologous protein SNAP30 [Cucurbita moschata] >XP_022921396.1 p... [more]
XP_023516514.12.7e-14789.32putative SNAP25 homologous protein SNAP30 [Cucurbita pepo subsp. pepo] >XP_02351... [more]
Match NameE-valueIdentityDescription
Q9S7P92.1e-7856.13SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 ... [more]
Q9LMG85.6e-7165.00Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNA... [more]
Q9SD964.2e-5851.59SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 ... [more]
Q17QQ34.8e-1428.64Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1[more]
P608784.8e-1428.64Synaptosomal-associated protein 25 OS=Gallus gallus OX=9031 GN=SNAP25 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LRS53.0e-15293.20t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=365... [more]
A0A5A7US664.8e-15092.88Putative SNAP25-like proteinous protein SNAP30 OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3BVT24.8e-15092.88putative SNAP25 homologous protein SNAP30 OS=Cucumis melo OX=3656 GN=LOC10349402... [more]
A0A6J1E1975.9e-14889.64putative SNAP25 homologous protein SNAP30 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1JJU07.2e-14689.00putative SNAP25 homologous protein SNAP30 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT5G61210.11.5e-7956.13soluble N-ethylmaleimide-sensitive factor adaptor protein 33 [more]
AT1G13890.14.0e-7265.00soluble N-ethylmaleimide-sensitive factor adaptor protein 30 [more]
AT5G07880.13.0e-5951.59synaptosomal-associated protein SNAP25-like 29 [more]
AT3G09740.16.1e-0437.14syntaxin of plants 71 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 281..308
NoneNo IPR availableCOILSCoilCoilcoord: 128..148
NoneNo IPR availableGENE3D1.20.5.110coord: 100..177
e-value: 2.2E-20
score: 74.4
NoneNo IPR availableGENE3D1.20.5.110coord: 228..308
e-value: 1.5E-20
score: 74.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 46..60
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..97
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 11..30
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 73..89
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 209..238
NoneNo IPR availablePANTHERPTHR19305:SF30TARGET SNARE COILED-COIL DOMAIN-CONTAINING PROTEIN-RELATEDcoord: 22..308
NoneNo IPR availablePANTHERPTHR19305SYNAPTOSOMAL ASSOCIATED PROTEINcoord: 22..308
NoneNo IPR availableCDDcd15841SNARE_Qccoord: 248..304
e-value: 4.10983E-12
score: 58.3388
NoneNo IPR availableSUPERFAMILY58038SNARE fusion complexcoord: 104..174
NoneNo IPR availableSUPERFAMILY58038SNARE fusion complexcoord: 246..306
IPR000727Target SNARE coiled-coil homology domainSMARTSM00397tSNARE_6coord: 238..305
e-value: 3.0E-8
score: 43.4
coord: 109..176
e-value: 12.0
score: 3.1
IPR000727Target SNARE coiled-coil homology domainPROSITEPS50192T_SNAREcoord: 248..305
score: 14.072053
IPR044766NPSN/SNAP25-like, N-terminal SNARE domainCDDcd15861SNARE_SNAP25N_23N_29N_SEC9Ncoord: 111..175
e-value: 1.59511E-20
score: 81.4655

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10008550.1HG10008550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0061025 membrane fusion
biological_process GO:0015031 protein transport
cellular_component GO:0005886 plasma membrane
cellular_component GO:0031201 SNARE complex
molecular_function GO:0005484 SNAP receptor activity