Homology
BLAST of HG10008538 vs. NCBI nr
Match:
XP_038878698.1 (flavonoid 3'-monooxygenase [Benincasa hispida])
HSP 1 Score: 920.2 bits (2377), Expect = 7.8e-264
Identity = 466/519 (89.79%), Postives = 482/519 (92.87%), Query Frame = 0
Query: 1 MDEISLIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
MDEI+LIVAA VVTLA ILLHR FF R RF LPPGPKPWPIVGNMPQIGGAPLHSLLAA
Sbjct: 1 MDEIALIVAA-VVTLASTILLHRTFFRRSRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
Query: 61 MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 120
MAGEYG LMYLRLGSV VVVAASA+VAE+LLK+H TNFLSRPPNAGAKYIAYN +DMVFA
Sbjct: 61 MAGEYGSLMYLRLGSVGVVVAASAAVAEKLLKVHDTNFLSRPPNAGAKYIAYNYQDMVFA 120
Query: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 180
PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEI VLVQALA SGE PA LGSLLTVCTA
Sbjct: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIAVLVQALASSGEVPAALGSLLTVCTA 180
Query: 181 NVLGRTMISRRVFGD-GSGGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVAS 240
N LGRTMI RRVFGD GSG D EA FKAMVEQVM+L GKFNPGDFFPSLEWLDLLGVAS
Sbjct: 181 NALGRTMIGRRVFGDGGSGDDSEAKQFKAMVEQVMILAGKFNPGDFFPSLEWLDLLGVAS 240
Query: 241 EMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDSEIK 300
EMKKVHKWFDDFLTKIVEEHRNLLA G GG QDLLSTLLSS AEDENEK+ND+EIK
Sbjct: 241 EMKKVHKWFDDFLTKIVEEHRNLLARGRG-GGHQDLLSTLLSS---AEDENEKINDTEIK 300
Query: 301 ALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYL 360
ALLLNMFTAGTDTSASTVEWALTELIRHPK+MA+AQ+ELD+VVGRDRAVSDLDL QL YL
Sbjct: 301 ALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSVVGRDRAVSDLDLPQLVYL 360
Query: 361 QAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFR 420
QAVVKETFRLHPPTPLS+PRMAAESCEINGYHIPKG+TLLVD+WAI RDPKEWADPLEFR
Sbjct: 361 QAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGTTLLVDVWAIGRDPKEWADPLEFR 420
Query: 421 PNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPNG 480
PNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATL H FDWALPNG
Sbjct: 421 PNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLVHYFDWALPNG 480
Query: 481 LLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQKLP 519
LLPD+LNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQK P
Sbjct: 481 LLPDELNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQKSP 514
BLAST of HG10008538 vs. NCBI nr
Match:
XP_008453263.1 (PREDICTED: flavonoid 3'-monooxygenase [Cucumis melo] >KAA0057987.1 flavonoid 3'-monooxygenase [Cucumis melo var. makuwa])
HSP 1 Score: 904.0 bits (2335), Expect = 5.8e-259
Identity = 452/522 (86.59%), Postives = 479/522 (91.76%), Query Frame = 0
Query: 1 MDEISLIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
M+EI+LIVAA ++TLA A LLHR FFLRRRF LPPGPKPWPIVGNMPQIGGAPLHSLLAA
Sbjct: 1 MEEITLIVAA-IITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
Query: 61 MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 120
MAG+YGPLMYLRLGSVD+VVAASA+VAEQ+LKIH TNFLSRPPNAGAKYIAYN +DMVFA
Sbjct: 61 MAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 120
Query: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 180
PYGPRWRLLRKIS VHLFSSKALDDHRRIR++EI VLVQALARSGEAPA LGSLLTVC A
Sbjct: 121 PYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIA 180
Query: 181 NVLGRTMISRRVFGDGSGGDP-EANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVAS 240
N LGRTMI RRVFGDGSG D E+N FKAMVEQVMVL GKFNPGDFFP LE LDL+GV S
Sbjct: 181 NSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGS 240
Query: 241 EMKKVHKWFDDFLTKIVEEHRNLLA---GGGDRGGDQDLLSTLLSSKDEAEDENEKLNDS 300
EMKKVHKWFDDFLTKIVEEHRNLLA GG GG QDLLSTLLS KDE DENEKLND+
Sbjct: 241 EMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDT 300
Query: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQL 360
EIKALLLNMFTAGTDTSASTVEWALTELIRHP++MA+AQ+ELD+VVGRDRAVSDLDL QL
Sbjct: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQL 360
Query: 361 PYLQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPL 420
YLQAVVKETFRLHPPTPLS+PRMA++SCE+NGYHIPKGSTLLVD+WAI RDPKEW DPL
Sbjct: 361 VYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPL 420
Query: 421 EFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWAL 480
EF PNRFLP GEK HVDVKGNDFE IPFGAGRRICVG+S+GLRMVQMLTAT+ HSFDWAL
Sbjct: 421 EFGPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWAL 480
Query: 481 PNGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQKLP 519
PNGL PDKLNMDE YGLTLRRAQPLVVHPRPRLL GVYQKLP
Sbjct: 481 PNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP 521
BLAST of HG10008538 vs. NCBI nr
Match:
XP_004138192.1 (flavonoid 3'-monooxygenase [Cucumis sativus] >KGN63725.2 hypothetical protein Csa_014030 [Cucumis sativus])
HSP 1 Score: 889.0 bits (2296), Expect = 1.9e-254
Identity = 441/517 (85.30%), Postives = 474/517 (91.68%), Query Frame = 0
Query: 1 MDEISLIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
M+EI+ IVAA ++TLA A LLHRAFFLRRR LPPGPKPWPIVGNMPQIG A LHSLLA
Sbjct: 1 MEEITPIVAA-LITLASAFLLHRAFFLRRRLRLPPGPKPWPIVGNMPQIGEAHLHSLLAT 60
Query: 61 MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 120
MAG+YGPLMYLRLGSVD+VVAASA+VAEQ+LKIH TNFLSRPPNAGAKYIAYN +DMVFA
Sbjct: 61 MAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 120
Query: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 180
PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEI VLVQALARSGEAPA LGSLLTVC A
Sbjct: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVCIA 180
Query: 181 NVLGRTMISRRVFGDGSGGDP-EANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVAS 240
N LGRTMI RRVFGDGSG D E+N FK MVEQVMVL GKFNPGDFFP LEWLDL+GV
Sbjct: 181 NSLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGR 240
Query: 241 EMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDSEIK 300
EMKKVHKWFDDFLTKIVEEHRNLLA G GG QDLLSTLLS KD+ EDENEKLND+EIK
Sbjct: 241 EMKKVHKWFDDFLTKIVEEHRNLLARGVG-GGHQDLLSTLLSMKDDGEDENEKLNDTEIK 300
Query: 301 ALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYL 360
ALLLNMFTAGTDTSASTVEWALTELIRHP++MA+AQ+ELD++VGRDRAVSD+DL QL YL
Sbjct: 301 ALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSIVGRDRAVSDVDLHQLVYL 360
Query: 361 QAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFR 420
QAVVKETFRLHPPTPLS+PRMA++SCE+NGYHIPKGSTLLVD+WAI RDPK+W DPLEFR
Sbjct: 361 QAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEFR 420
Query: 421 PNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPNG 480
PNRFLP GEK HVDVKGNDFEVIPFGAGRRICVG+S+GLRMVQMLTAT+ HSFDW LPNG
Sbjct: 421 PNRFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPNG 480
Query: 481 LLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQK 517
L PDKLNMDE YGLTLRRAQPL++HPRPRLL GVY++
Sbjct: 481 LTPDKLNMDEHYGLTLRRAQPLIMHPRPRLLLGVYER 515
BLAST of HG10008538 vs. NCBI nr
Match:
XP_023529494.1 (flavonoid 3'-monooxygenase [Cucurbita pepo subsp. pepo] >XP_023529495.1 flavonoid 3'-monooxygenase [Cucurbita pepo subsp. pepo])
HSP 1 Score: 870.2 bits (2247), Expect = 9.3e-249
Identity = 433/521 (83.11%), Postives = 470/521 (90.21%), Query Frame = 0
Query: 1 MDEISLIVAAVVVTLAYAILLHRAFFLRRR---FCLPPGPKPWPIVGNMPQIGGAPLHSL 60
MDEISL +A +TLA +LL+R F +R LPPGPKPWPIVGN+PQIGGAPLHSL
Sbjct: 1 MDEISLFFSA-AITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSL 60
Query: 61 LAAMAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDM 120
LA M+GEYGPLMYLR GSVDVVVAASA++AEQ LKI TNFLSRPPNAGAKYIAYN +DM
Sbjct: 61 LADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDM 120
Query: 121 VFAPYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTV 180
VFAPYGPRWRLLRKIS VHLFSSKAL+DHRR+REEE+GVLVQALARSGEAPATLGSLLTV
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTV 180
Query: 181 CTANVLGRTMISRRVFGDGS-GGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLG 240
CTAN LGRTMI RRVFGDGS GGDP ++ FKAMVEQVM+L GKFNPGDFFPSL+WLDLLG
Sbjct: 181 CTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFPSLDWLDLLG 240
Query: 241 VASEMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGG--DQDLLSTLLSSKDEAEDENEKLN 300
VA EMKKVHKWFDD L+KI+EEHRNLLA GG GG QDLLSTLLS++D AEDENEKLN
Sbjct: 241 VAREMKKVHKWFDDLLSKILEEHRNLLASGGGGGGGEHQDLLSTLLSARDRAEDENEKLN 300
Query: 301 DSEIKALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLS 360
D+EIKALLLNMFTAGTDTSASTVEWAL ELIRHPK+M +AQ+ELD++VGRDR VSDLDL
Sbjct: 301 DTEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMHQAQQELDSIVGRDRVVSDLDLP 360
Query: 361 QLPYLQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWAD 420
+L YLQAVVKETFRLHPPTPLS+PRMAAESCEI+GY IPKGSTLLVD+WAISRDPKEW
Sbjct: 361 KLGYLQAVVKETFRLHPPTPLSLPRMAAESCEISGYRIPKGSTLLVDVWAISRDPKEWTS 420
Query: 421 PLEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDW 480
PLEFRP+RFLPGGEKAHVDVKGNDF VIPFGAGRRICVGMSMGLRMVQ+LTA+L HSFDW
Sbjct: 421 PLEFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQILTASLVHSFDW 480
Query: 481 ALPNGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQ 516
ALP+GL P +LNMDEA+GLTLRRAQPLV HPRPRL PGVYQ
Sbjct: 481 ALPSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 520
BLAST of HG10008538 vs. NCBI nr
Match:
XP_022933611.1 (flavonoid 3'-monooxygenase [Cucurbita moschata])
HSP 1 Score: 865.9 bits (2236), Expect = 1.7e-247
Identity = 432/519 (83.24%), Postives = 468/519 (90.17%), Query Frame = 0
Query: 1 MDEISLIVAAVVVTLAYAILLHRAFFLRRR---FCLPPGPKPWPIVGNMPQIGGAPLHSL 60
MDEISL +A +TLA +LL+R F +R LPPGPKPWPIVGN+PQIGGAPLHSL
Sbjct: 1 MDEISLFFSA-AITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSL 60
Query: 61 LAAMAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDM 120
LA M+GEYGPLMYLR GSVDVVVAASA++AEQ LKI TNFLSRPPNAGAKYIAYN +DM
Sbjct: 61 LADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDM 120
Query: 121 VFAPYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTV 180
VFAPYGPRWRLLRKIS VHLFSSKAL+DHRR+REEE+GVLVQALARSGEAPATLGSLLTV
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTV 180
Query: 181 CTANVLGRTMISRRVFGDGS-GGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLG 240
CTAN LGRTMI RRVFGDGS GGDP ++ FKAMVEQVM+L GKFNPGDFF LEWLDLLG
Sbjct: 181 CTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFSWLEWLDLLG 240
Query: 241 VASEMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDS 300
VA EMKKVHKWFDD ++KI+EEHRNLLA GG G QDLLSTLLS++D A+DENEKLND+
Sbjct: 241 VAGEMKKVHKWFDDLMSKILEEHRNLLATGGG-GEHQDLLSTLLSTRDRAKDENEKLNDT 300
Query: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQL 360
EIKALLLNMFTAGTDTSASTVEWAL ELIRHPK+M +AQ+ELD+VVGRDR VSDLDL +L
Sbjct: 301 EIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKL 360
Query: 361 PYLQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPL 420
YLQAVVKETFRLHPPTPLS+PRMAAESCEI+GYHIPKGSTLLVD+WAISRDPKEW PL
Sbjct: 361 GYLQAVVKETFRLHPPTPLSLPRMAAESCEISGYHIPKGSTLLVDVWAISRDPKEWTSPL 420
Query: 421 EFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWAL 480
EFRP+RFLPGGEKAHVDVKGNDF VIPFGAGRRICVGMSMGLRMVQMLTA+L HSFDWAL
Sbjct: 421 EFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWAL 480
Query: 481 PNGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQ 516
P+GL P +LNMDEA+GLTLRRAQPLV HPRPRL PGVYQ
Sbjct: 481 PSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 517
BLAST of HG10008538 vs. ExPASy Swiss-Prot
Match:
Q9SBQ9 (Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1)
HSP 1 Score: 664.5 bits (1713), Expect = 1.0e-189
Identity = 328/516 (63.57%), Postives = 406/516 (78.68%), Query Frame = 0
Query: 1 MDEISLIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
M+ +SLI+ V+ + +L F R LPPGPKPWPI+GN+ +G P H AA
Sbjct: 1 MEILSLILYTVIFSFLLQFILRSFFRKRYPLPLPPGPKPWPIIGNLVHLGPKP-HQSTAA 60
Query: 61 MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 120
MA YGPLMYL++G VDVVVAASASVA Q LK H NF SRPPN+GA+++AYN +D+VFA
Sbjct: 61 MAQTYGPLMYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQDLVFA 120
Query: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 180
PYGPRWR+LRKI +VHLFS+KALDD R +R++E+ L +ALA +G+ P LG LL VCT
Sbjct: 121 PYGPRWRMLRKICSVHLFSTKALDDFRHVRQDEVKTLTRALASAGQKPVKLGQLLNVCTT 180
Query: 181 NVLGRTMISRRVFGDGSGG-DPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVAS 240
N L R M+ +RVF DGSG DP+A FK+MV ++MV+ G FN GDF P L WLD+ GVA+
Sbjct: 181 NALARVMLGKRVFADGSGDVDPQAAEFKSMVVEMMVVAGVFNIGDFIPQLNWLDIQGVAA 240
Query: 241 EMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSK-DEAEDENEKLNDSEI 300
+MKK+H FD FLT I+EEH+ G G +DLLSTL+S K D+A+++ KL D+EI
Sbjct: 241 KMKKLHARFDAFLTDILEEHK-----GKIFGEMKDLLSTLISLKNDDADNDGGKLTDTEI 300
Query: 301 KALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPY 360
KALLLN+F AGTDTS+STVEWA+ ELIR+PKI+A+AQ+E+D VVGRDR V +LDL+QL Y
Sbjct: 301 KALLLNLFVAGTDTSSSTVEWAIAELIRNPKILAQAQQEIDKVVGRDRLVGELDLAQLTY 360
Query: 361 LQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEF 420
L+A+VKETFRLHP TPLS+PR+A+ESCEINGY IPKGSTLL+++WAI+RDP WADPLEF
Sbjct: 361 LEAIVKETFRLHPSTPLSLPRIASESCEINGYFIPKGSTLLLNVWAIARDPNAWADPLEF 420
Query: 421 RPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPN 480
RP RFLPGGEK VDV+GNDFEVIPFGAGRRIC GM++G+RMVQ++ ATL H+F+W L +
Sbjct: 421 RPERFLPGGEKPKVDVRGNDFEVIPFGAGRRICAGMNLGIRMVQLMIATLIHAFNWDLVS 480
Query: 481 GLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVY 515
G LP+ LNM+EAYGLTL+RA PLVVHPRPRL Y
Sbjct: 481 GQLPEMLNMEEAYGLTLQRADPLVVHPRPRLEAQAY 510
BLAST of HG10008538 vs. ExPASy Swiss-Prot
Match:
Q9SD85 (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP75B1 PE=1 SV=1)
HSP 1 Score: 643.7 bits (1659), Expect = 1.8e-183
Identity = 318/515 (61.75%), Postives = 396/515 (76.89%), Query Frame = 0
Query: 5 SLIVAAVVVTLAYAILLHRAFFLRRRFC----LPPGPKPWPIVGNMPQIGGAPLHSLLAA 64
+L + ++ T+ + IL R F RR LPPGP PWPI+GN+P +G P H L+A
Sbjct: 3 TLFLTILLATVLFLIL--RIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKP-HRTLSA 62
Query: 65 MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 124
M YGP+++LRLG VDVVVAAS SVAEQ LKIH NF SRPPN+GAK++AYN +D+VFA
Sbjct: 63 MVTTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFA 122
Query: 125 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 184
PYG RWRLLRKIS+VHLFS+KAL+D + +R+EE+G L + L R G P LG L+ +C
Sbjct: 123 PYGHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVV 182
Query: 185 NVLGRTMISRRVFGDGSGGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVASE 244
N LGR MI RR+F G+ D +A+ F++MV ++M L G FN GDF PSL+WLDL GVA +
Sbjct: 183 NALGREMIGRRLF--GADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGK 242
Query: 245 MKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSK-DEAEDENEKLNDSEIK 304
MK++HK FD FL+ I++EH G D+LSTL+S K + + + L D+EIK
Sbjct: 243 MKRLHKRFDAFLSSILKEHEM----NGQDQKHTDMLSTLISLKGTDLDGDGGSLTDTEIK 302
Query: 305 ALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYL 364
ALLLNMFTAGTDTSASTV+WA+ ELIRHP IM +AQ+ELD VVGRDR V++ D++QLPYL
Sbjct: 303 ALLLNMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYL 362
Query: 365 QAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFR 424
QAV+KE FRLHPPTPLS+P +A+ESCEINGYHIPKGSTLL +IWAI+RDP +W+DPL F+
Sbjct: 363 QAVIKENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFK 422
Query: 425 PNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPNG 484
P RFLPGGEK+ VDVKG+DFE+IPFGAGRRIC G+S+GLR +Q LTATL FDW L G
Sbjct: 423 PERFLPGGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGG 482
Query: 485 LLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVY 515
+ P+KLNM+E+YGLTL+RA PLVVHP+PRL P VY
Sbjct: 483 VTPEKLNMEESYGLTLQRAVPLVVHPKPRLAPNVY 508
BLAST of HG10008538 vs. ExPASy Swiss-Prot
Match:
A0A4D6Q415 (Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B137 PE=1 SV=1)
HSP 1 Score: 584.3 bits (1505), Expect = 1.3e-165
Identity = 293/516 (56.78%), Postives = 373/516 (72.29%), Query Frame = 0
Query: 5 SLIVAAVVVTLAYAILLHRAFFLRRRFC----LPPGPKPWPIVGNMPQIGGAPLHSLLAA 64
+L++++ +V L Y RRR LPPGP WPI+GN+PQ+G P H L A
Sbjct: 11 TLLLSSFIVYLLY----------RRRSAQCPPLPPGPNGWPILGNLPQLGAKP-HQTLDA 70
Query: 65 MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 124
++ +YGPL LRLGSV+VVVA+S++VA Q L+ H NF +RPPN+GA+++AYN +D+VFA
Sbjct: 71 LSKQYGPLFRLRLGSVNVVVASSSAVAAQFLRTHDVNFSNRPPNSGAEHVAYNYQDLVFA 130
Query: 125 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAP--ATLGSLLTVC 184
PYGPRWR+LRK+ +VHLFS KALDD R +R+ E+ LV+ L R + LG L VC
Sbjct: 131 PYGPRWRMLRKLCSVHLFSLKALDDLRPVRQGEVACLVRNLRRHADTGVLVNLGKALNVC 190
Query: 185 TANVLGRTMISRRVFGDGSGGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVA 244
N L R M+ RRVF D EA+ FK MV ++M L G FN GDF P L WLDL GV
Sbjct: 191 ATNALARAMLGRRVFADEDAQLAEADEFKEMVVELMRLAGVFNVGDFVPGLGWLDLQGVV 250
Query: 245 SEMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDSEI 304
+MK++H+ +D FL +++EE++ G DLLS L+ K+ + KLN+++I
Sbjct: 251 GKMKRLHRRYDAFLDRVIEENQANAKSG-------DLLSVLIRLKEADAEGEIKLNNTDI 310
Query: 305 KALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPY 364
KALLLN+FTAGTDTS+STVEW L ELIRHP I+ + Q ELD+V+GRDR V++ DL LPY
Sbjct: 311 KALLLNLFTAGTDTSSSTVEWVLAELIRHPDILQKTQHELDSVIGRDRLVAESDLPNLPY 370
Query: 365 LQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEF 424
LQAVVKETFRLHP TPLS+PRMA+E C +NGY IPK +TLLV++W+I RD W DPLEF
Sbjct: 371 LQAVVKETFRLHPSTPLSLPRMASEECIVNGYKIPKHATLLVNVWSIGRDAAVWNDPLEF 430
Query: 425 RPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPN 484
RP+RFLPGGE+ HVDVKGNDFEVIPFGAGRRIC G+S+GLRMVQ +TAT+ H++DW+LP
Sbjct: 431 RPSRFLPGGEREHVDVKGNDFEVIPFGAGRRICAGLSLGLRMVQFMTATIVHAYDWSLPK 490
Query: 485 GLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVY 515
G KL+M+EAYGLTL+RA PL+V P PRL Y
Sbjct: 491 GQECQKLDMEEAYGLTLQRAVPLMVQPIPRLSHKAY 508
BLAST of HG10008538 vs. ExPASy Swiss-Prot
Match:
Q7G602 (Flavonoid 3'-monooxygenase CYP75B3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP75B3 PE=2 SV=1)
HSP 1 Score: 575.5 bits (1482), Expect = 6.2e-163
Identity = 284/493 (57.61%), Postives = 358/493 (72.62%), Query Frame = 0
Query: 28 RRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAAMAGEYGPLMYLRLGSVDVVVAASASVA 87
R+R LPPGP+ WP++GN+PQ+G P H + A+A +YGPL LR G +VVVAASA VA
Sbjct: 36 RKRRPLPPGPRGWPVLGNLPQLGDKP-HHTMCALARQYGPLFRLRFGCAEVVVAASAPVA 95
Query: 88 EQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFAPYGPRWRLLRKISTVHLFSSKALDDHR 147
Q L+ H NF +RPPN+GA+++AYN +D+VFAPYG RWR LRK+ +HLFS+KALDD R
Sbjct: 96 AQFLRGHDANFSNRPPNSGAEHVAYNYQDLVFAPYGARWRALRKLCALHLFSAKALDDLR 155
Query: 148 RIREEEIGVLVQALARSGEAPATLGSLLTVCTANVLGRTMISRRVFG-DGSGGDPEANIF 207
+RE E+ ++V+ LAR A LG VC N L R I RVF DG G A F
Sbjct: 156 AVREGEVALMVRNLARQQAASVALGQEANVCATNTLARATIGHRVFAVDGGEG---AREF 215
Query: 208 KAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVASEMKKVHKWFDDFLTKIVEEHRNLLAGG 267
K MV ++M L G FN GDF P+L WLD GV ++MK++H+ +D+ + + E +
Sbjct: 216 KEMVVELMQLAGVFNVGDFVPALRWLDPQGVVAKMKRLHRRYDNMMNGFINERKAGAQPD 275
Query: 268 GDRGGD--QDLLSTLLSSKDE---AEDENEKLNDSEIKALLLNMFTAGTDTSASTVEWAL 327
G G+ DLLS LL+ E + + EK+ +++IKALLLN+FTAGTDT++STVEWAL
Sbjct: 276 GVAAGEHGNDLLSVLLARMQEEQKLDGDGEKITETDIKALLLNLFTAGTDTTSSTVEWAL 335
Query: 328 TELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYLQAVVKETFRLHPPTPLSVPRMA 387
ELIRHP ++ EAQ ELDTVVGR R VS+ DL +LPYL AV+KETFRLHP TPLS+PR A
Sbjct: 336 AELIRHPDVLKEAQHELDTVVGRGRLVSESDLPRLPYLTAVIKETFRLHPSTPLSLPREA 395
Query: 388 AESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFRPNRFLPGGEKAHVDVKGNDFEV 447
AE CE++GY IPKG+TLLV++WAI+RDP +W DPL+++P+RFLPG A VDVKG DF +
Sbjct: 396 AEECEVDGYRIPKGATLLVNVWAIARDPTQWPDPLQYQPSRFLPGRMHADVDVKGADFGL 455
Query: 448 IPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPNGLLPDKLNMDEAYGLTLRRAQPL 507
IPFGAGRRIC G+S GLRMV ++TATL H FDW L NG PDKLNM+EAYGLTL+RA PL
Sbjct: 456 IPFGAGRRICAGLSWGLRMVTLMTATLVHGFDWTLANGATPDKLNMEEAYGLTLQRAVPL 515
Query: 508 VVHPRPRLLPGVY 515
+V P PRLLP Y
Sbjct: 516 MVQPVPRLLPSAY 524
BLAST of HG10008538 vs. ExPASy Swiss-Prot
Match:
Q8LM92 (Flavonoid 3'-monooxygenase CYP75B4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP75B4 PE=2 SV=1)
HSP 1 Score: 560.8 bits (1444), Expect = 1.6e-158
Identity = 285/522 (54.60%), Postives = 359/522 (68.77%), Query Frame = 0
Query: 6 LIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAAMAGEY 65
L A+ V + YA++ RA R LPPGP+ WP++GN+PQ+GG H L M Y
Sbjct: 14 LTTVALSVIVCYALVFSRAGKARAPLPLPPGPRGWPVLGNLPQLGG-KTHQTLHEMTKVY 73
Query: 66 GPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFAPYGPR 125
GPL+ LR GS DVVVA SA VA Q L+ H NF SRP N+G +++AYN RD+VF PYGPR
Sbjct: 74 GPLIRLRFGSSDVVVAGSAPVAAQFLRTHDANFSSRPRNSGGEHMAYNGRDVVFGPYGPR 133
Query: 126 WRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPAT-------LGSLLTVC 185
WR +RKI V+LFS++ALDD R RE E ++V++LA + AP + LG + VC
Sbjct: 134 WRAMRKICAVNLFSARALDDLRAFREREAVLMVRSLAEASAAPGSSSPAAVVLGKEVNVC 193
Query: 186 TANVLGRTMISRRVFGDGSGGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVA 245
T N L R + RRVF G+G A FK +V +VM + G N GDF P+L WLD GV
Sbjct: 194 TTNALSRAAVGRRVFAAGAG--EGAREFKEIVLEVMEVGGVLNVGDFVPALRWLDPQGVV 253
Query: 246 SEMKKVHKWFDDFLTKIVEEHR--NLLAGGGDRGGDQDLLSTLLSSKDEAE----DENEK 305
+ MKK+H+ FDD + I+ E R +LL R +DLL LL+ E E E+++
Sbjct: 254 ARMKKLHRRFDDMMNAIIAERRAGSLLKPTDSREEGKDLLGLLLAMVQEQEWLAAGEDDR 313
Query: 306 LNDSEIKALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLD 365
+ D+EIKAL+LN+F AGTDT+++ VEW + ELIRHP I+ AQ+ELD VVGRDR +S+ D
Sbjct: 314 ITDTEIKALILNLFVAGTDTTSTIVEWTMAELIRHPDILKHAQEELDVVVGRDRLLSESD 373
Query: 366 LSQLPYLQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEW 425
LS L + A++KETFRLHP TPLS+PRMA+E CEI GY IPKG+ LLV++W I+RDP W
Sbjct: 374 LSHLTFFHAIIKETFRLHPSTPLSLPRMASEECEIAGYRIPKGAELLVNVWGIARDPAIW 433
Query: 426 ADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSF 485
DPLE++P+RFLPGG VDVKGNDF +IPFGAGRRIC G+S GLRMV M ATL H+F
Sbjct: 434 PDPLEYKPSRFLPGGTHTDVDVKGNDFGLIPFGAGRRICAGLSWGLRMVTMTAATLVHAF 493
Query: 486 DWALPNGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVY 515
DW LP PDKLNMDEA+ L L+RA+PLVVHP PRLLP Y
Sbjct: 494 DWQLPADQTPDKLNMDEAFTLLLQRAEPLVVHPVPRLLPSAY 532
BLAST of HG10008538 vs. ExPASy TrEMBL
Match:
A0A5A7UTD5 (Flavonoid 3'-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002910 PE=3 SV=1)
HSP 1 Score: 904.0 bits (2335), Expect = 2.8e-259
Identity = 452/522 (86.59%), Postives = 479/522 (91.76%), Query Frame = 0
Query: 1 MDEISLIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
M+EI+LIVAA ++TLA A LLHR FFLRRRF LPPGPKPWPIVGNMPQIGGAPLHSLLAA
Sbjct: 1 MEEITLIVAA-IITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
Query: 61 MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 120
MAG+YGPLMYLRLGSVD+VVAASA+VAEQ+LKIH TNFLSRPPNAGAKYIAYN +DMVFA
Sbjct: 61 MAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 120
Query: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 180
PYGPRWRLLRKIS VHLFSSKALDDHRRIR++EI VLVQALARSGEAPA LGSLLTVC A
Sbjct: 121 PYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIA 180
Query: 181 NVLGRTMISRRVFGDGSGGDP-EANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVAS 240
N LGRTMI RRVFGDGSG D E+N FKAMVEQVMVL GKFNPGDFFP LE LDL+GV S
Sbjct: 181 NSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGS 240
Query: 241 EMKKVHKWFDDFLTKIVEEHRNLLA---GGGDRGGDQDLLSTLLSSKDEAEDENEKLNDS 300
EMKKVHKWFDDFLTKIVEEHRNLLA GG GG QDLLSTLLS KDE DENEKLND+
Sbjct: 241 EMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDT 300
Query: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQL 360
EIKALLLNMFTAGTDTSASTVEWALTELIRHP++MA+AQ+ELD+VVGRDRAVSDLDL QL
Sbjct: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQL 360
Query: 361 PYLQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPL 420
YLQAVVKETFRLHPPTPLS+PRMA++SCE+NGYHIPKGSTLLVD+WAI RDPKEW DPL
Sbjct: 361 VYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPL 420
Query: 421 EFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWAL 480
EF PNRFLP GEK HVDVKGNDFE IPFGAGRRICVG+S+GLRMVQMLTAT+ HSFDWAL
Sbjct: 421 EFGPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWAL 480
Query: 481 PNGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQKLP 519
PNGL PDKLNMDE YGLTLRRAQPLVVHPRPRLL GVYQKLP
Sbjct: 481 PNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP 521
BLAST of HG10008538 vs. ExPASy TrEMBL
Match:
A0A1S3BVU8 (flavonoid 3'-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103494036 PE=3 SV=1)
HSP 1 Score: 904.0 bits (2335), Expect = 2.8e-259
Identity = 452/522 (86.59%), Postives = 479/522 (91.76%), Query Frame = 0
Query: 1 MDEISLIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
M+EI+LIVAA ++TLA A LLHR FFLRRRF LPPGPKPWPIVGNMPQIGGAPLHSLLAA
Sbjct: 1 MEEITLIVAA-IITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
Query: 61 MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 120
MAG+YGPLMYLRLGSVD+VVAASA+VAEQ+LKIH TNFLSRPPNAGAKYIAYN +DMVFA
Sbjct: 61 MAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 120
Query: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 180
PYGPRWRLLRKIS VHLFSSKALDDHRRIR++EI VLVQALARSGEAPA LGSLLTVC A
Sbjct: 121 PYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIA 180
Query: 181 NVLGRTMISRRVFGDGSGGDP-EANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVAS 240
N LGRTMI RRVFGDGSG D E+N FKAMVEQVMVL GKFNPGDFFP LE LDL+GV S
Sbjct: 181 NSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGS 240
Query: 241 EMKKVHKWFDDFLTKIVEEHRNLLA---GGGDRGGDQDLLSTLLSSKDEAEDENEKLNDS 300
EMKKVHKWFDDFLTKIVEEHRNLLA GG GG QDLLSTLLS KDE DENEKLND+
Sbjct: 241 EMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDT 300
Query: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQL 360
EIKALLLNMFTAGTDTSASTVEWALTELIRHP++MA+AQ+ELD+VVGRDRAVSDLDL QL
Sbjct: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQL 360
Query: 361 PYLQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPL 420
YLQAVVKETFRLHPPTPLS+PRMA++SCE+NGYHIPKGSTLLVD+WAI RDPKEW DPL
Sbjct: 361 VYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPL 420
Query: 421 EFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWAL 480
EF PNRFLP GEK HVDVKGNDFE IPFGAGRRICVG+S+GLRMVQMLTAT+ HSFDWAL
Sbjct: 421 EFGPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWAL 480
Query: 481 PNGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQKLP 519
PNGL PDKLNMDE YGLTLRRAQPLVVHPRPRLL GVYQKLP
Sbjct: 481 PNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP 521
BLAST of HG10008538 vs. ExPASy TrEMBL
Match:
A0A6J1EZJ7 (flavonoid 3'-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111440987 PE=3 SV=1)
HSP 1 Score: 865.9 bits (2236), Expect = 8.5e-248
Identity = 432/519 (83.24%), Postives = 468/519 (90.17%), Query Frame = 0
Query: 1 MDEISLIVAAVVVTLAYAILLHRAFFLRRR---FCLPPGPKPWPIVGNMPQIGGAPLHSL 60
MDEISL +A +TLA +LL+R F +R LPPGPKPWPIVGN+PQIGGAPLHSL
Sbjct: 1 MDEISLFFSA-AITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSL 60
Query: 61 LAAMAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDM 120
LA M+GEYGPLMYLR GSVDVVVAASA++AEQ LKI TNFLSRPPNAGAKYIAYN +DM
Sbjct: 61 LADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDM 120
Query: 121 VFAPYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTV 180
VFAPYGPRWRLLRKIS VHLFSSKAL+DHRR+REEE+GVLVQALARSGEAPATLGSLLTV
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTV 180
Query: 181 CTANVLGRTMISRRVFGDGS-GGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLG 240
CTAN LGRTMI RRVFGDGS GGDP ++ FKAMVEQVM+L GKFNPGDFF LEWLDLLG
Sbjct: 181 CTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFSWLEWLDLLG 240
Query: 241 VASEMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDS 300
VA EMKKVHKWFDD ++KI+EEHRNLLA GG G QDLLSTLLS++D A+DENEKLND+
Sbjct: 241 VAGEMKKVHKWFDDLMSKILEEHRNLLATGGG-GEHQDLLSTLLSTRDRAKDENEKLNDT 300
Query: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQL 360
EIKALLLNMFTAGTDTSASTVEWAL ELIRHPK+M +AQ+ELD+VVGRDR VSDLDL +L
Sbjct: 301 EIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKL 360
Query: 361 PYLQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPL 420
YLQAVVKETFRLHPPTPLS+PRMAAESCEI+GYHIPKGSTLLVD+WAISRDPKEW PL
Sbjct: 361 GYLQAVVKETFRLHPPTPLSLPRMAAESCEISGYHIPKGSTLLVDVWAISRDPKEWTSPL 420
Query: 421 EFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWAL 480
EFRP+RFLPGGEKAHVDVKGNDF VIPFGAGRRICVGMSMGLRMVQMLTA+L HSFDWAL
Sbjct: 421 EFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWAL 480
Query: 481 PNGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQ 516
P+GL P +LNMDEA+GLTLRRAQPLV HPRPRL PGVYQ
Sbjct: 481 PSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 517
BLAST of HG10008538 vs. ExPASy TrEMBL
Match:
A0A6J1L0D6 (flavonoid 3'-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111499893 PE=3 SV=1)
HSP 1 Score: 860.5 bits (2222), Expect = 3.6e-246
Identity = 429/519 (82.66%), Postives = 466/519 (89.79%), Query Frame = 0
Query: 1 MDEISLIVAAVVVTLAYAILLHRAFFLRRR---FCLPPGPKPWPIVGNMPQIGGAPLHSL 60
MDEISL +A +TLA +LL+R F +R LPPGPKPWP VGN+PQIGGAPLHS
Sbjct: 1 MDEISLFFSA-AITLATVVLLYRLLFHQRNSRSLPLPPGPKPWPTVGNLPQIGGAPLHSQ 60
Query: 61 LAAMAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDM 120
LAAM+GEYGPLMYLR GS+DVVVAASA++AEQ LKI TNFLSRPPNAGAKYIAYN +DM
Sbjct: 61 LAAMSGEYGPLMYLRFGSIDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDM 120
Query: 121 VFAPYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTV 180
VFAPYGPRWRLLRKIS VHLFSSKAL+D+R +REEE+GVLVQALAR GEAPATLGSLLTV
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALEDNRCVREEEVGVLVQALARPGEAPATLGSLLTV 180
Query: 181 CTANVLGRTMISRRVFGDGS-GGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLG 240
CTAN LGRTMI RRVFGDGS GGDP ++ FKAMVEQVM+L GKFNPG+FFPSLEWLDLLG
Sbjct: 181 CTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGNFFPSLEWLDLLG 240
Query: 241 VASEMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDS 300
VA EMKKVHKWFDD L+KI+EEHR LLA GG+ QDLLSTLLS++D AEDENEKLND+
Sbjct: 241 VAREMKKVHKWFDDLLSKILEEHRTLLASGGEH---QDLLSTLLSTRDHAEDENEKLNDT 300
Query: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQL 360
EIKALLLNMFTAGTDTSASTVEWAL ELIRHPK+M +AQ+ELD+VVGRDR VSDLDL +L
Sbjct: 301 EIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKL 360
Query: 361 PYLQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPL 420
YLQAVVKETFRLHPPTPLS+PRMAAESCEINGYHIPKGSTLLVD+WAISRDPKEW PL
Sbjct: 361 GYLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAISRDPKEWTCPL 420
Query: 421 EFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWAL 480
EFRP+RFLPGGEKAHVDVKGNDF VIPFGAGRRICVGMSMGLRMVQMLTA+L HSFDWAL
Sbjct: 421 EFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWAL 480
Query: 481 PNGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQ 516
P+GL P +LNMDEA+GLTLRRAQPLV HPRPRL PGVYQ
Sbjct: 481 PSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 515
BLAST of HG10008538 vs. ExPASy TrEMBL
Match:
A0A0A0LP99 (Flavonoid 3' hydroxylase OS=Cucumis sativus OX=3659 GN=Csa_1G013190 PE=3 SV=1)
HSP 1 Score: 797.7 bits (2059), Expect = 2.8e-227
Identity = 404/508 (79.53%), Postives = 434/508 (85.43%), Query Frame = 0
Query: 1 MDEISLIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
M+EI+ IVAA ++TLA A LLHRAFFLRRR LPPGPKPWPIVGNMPQIG A LHSLLA
Sbjct: 1 MEEITPIVAA-LITLASAFLLHRAFFLRRRLRLPPGPKPWPIVGNMPQIGEAHLHSLLAT 60
Query: 61 MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 120
MAG+YGPLMYLRLGSVD+VVAASA+VAEQ+LKIH TNFLSRPPNAGAKYIAYN +DMVFA
Sbjct: 61 MAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 120
Query: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 180
PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEI VLVQALARSGEAPA LGSLLTVC A
Sbjct: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVCIA 180
Query: 181 NVLGRTMISRRVFGDGSGGDP-EANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVAS 240
N LGRTMI RRVFGDGSG D E+N FK MVEQVMVL GKFNPGDFFP LEWLDL+GV
Sbjct: 181 NSLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGR 240
Query: 241 EMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDSEIK 300
EMKKVHKWFDDFLTKIVEEHRNLLA G GG QDLLSTLLS KD+ EDENEKLND+EIK
Sbjct: 241 EMKKVHKWFDDFLTKIVEEHRNLLARGVG-GGHQDLLSTLLSMKDDGEDENEKLNDTEIK 300
Query: 301 ALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYL 360
ALLL +F ++ L+ +GRDRAVSD+DL QL YL
Sbjct: 301 ALLLVLFL--------------------------SKFYLNCFIGRDRAVSDVDLHQLVYL 360
Query: 361 QAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFR 420
QAVVKETFRLHPPTPLS+PRMA++SCE+NGYHIPKGSTLLVD+WAI RDPK+W DPLEFR
Sbjct: 361 QAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEFR 420
Query: 421 PNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPNG 480
PNRFLP GEK HVDVKGNDFEVIPFGAGRRICVG+S+GLRMVQMLTAT+ HSFDW LPNG
Sbjct: 421 PNRFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPNG 480
Query: 481 LLPDKLNMDEAYGLTLRRAQPLVVHPRP 508
L PDKLNMDE YGLTLRRAQPL++HPRP
Sbjct: 481 LTPDKLNMDEHYGLTLRRAQPLIMHPRP 480
BLAST of HG10008538 vs. TAIR 10
Match:
AT5G07990.1 (Cytochrome P450 superfamily protein )
HSP 1 Score: 643.7 bits (1659), Expect = 1.3e-184
Identity = 318/515 (61.75%), Postives = 396/515 (76.89%), Query Frame = 0
Query: 5 SLIVAAVVVTLAYAILLHRAFFLRRRFC----LPPGPKPWPIVGNMPQIGGAPLHSLLAA 64
+L + ++ T+ + IL R F RR LPPGP PWPI+GN+P +G P H L+A
Sbjct: 3 TLFLTILLATVLFLIL--RIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKP-HRTLSA 62
Query: 65 MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 124
M YGP+++LRLG VDVVVAAS SVAEQ LKIH NF SRPPN+GAK++AYN +D+VFA
Sbjct: 63 MVTTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFA 122
Query: 125 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 184
PYG RWRLLRKIS+VHLFS+KAL+D + +R+EE+G L + L R G P LG L+ +C
Sbjct: 123 PYGHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVV 182
Query: 185 NVLGRTMISRRVFGDGSGGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVASE 244
N LGR MI RR+F G+ D +A+ F++MV ++M L G FN GDF PSL+WLDL GVA +
Sbjct: 183 NALGREMIGRRLF--GADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGK 242
Query: 245 MKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSK-DEAEDENEKLNDSEIK 304
MK++HK FD FL+ I++EH G D+LSTL+S K + + + L D+EIK
Sbjct: 243 MKRLHKRFDAFLSSILKEHEM----NGQDQKHTDMLSTLISLKGTDLDGDGGSLTDTEIK 302
Query: 305 ALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYL 364
ALLLNMFTAGTDTSASTV+WA+ ELIRHP IM +AQ+ELD VVGRDR V++ D++QLPYL
Sbjct: 303 ALLLNMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYL 362
Query: 365 QAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFR 424
QAV+KE FRLHPPTPLS+P +A+ESCEINGYHIPKGSTLL +IWAI+RDP +W+DPL F+
Sbjct: 363 QAVIKENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFK 422
Query: 425 PNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPNG 484
P RFLPGGEK+ VDVKG+DFE+IPFGAGRRIC G+S+GLR +Q LTATL FDW L G
Sbjct: 423 PERFLPGGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGG 482
Query: 485 LLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVY 515
+ P+KLNM+E+YGLTL+RA PLVVHP+PRL P VY
Sbjct: 483 VTPEKLNMEESYGLTLQRAVPLVVHPKPRLAPNVY 508
BLAST of HG10008538 vs. TAIR 10
Match:
AT4G12310.1 (cytochrome P450, family 706, subfamily A, polypeptide 5 )
HSP 1 Score: 340.9 bits (873), Expect = 1.8e-93
Identity = 183/503 (36.38%), Postives = 286/503 (56.86%), Query Frame = 0
Query: 9 AAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAAMAGEYGPL 68
A +++T ++IL + F + LPPGP+ PIVGN+P + LH+ +A +GP+
Sbjct: 21 AVLILTATFSILWY-IFKRSPQPPLPPGPRGLPIVGNLPFL-DPDLHTYFTKLAQSHGPI 80
Query: 69 MYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFAPYGPRWRL 128
L LGS VV S S+A ++LK NF + A+ + Y D+V+ PYG WR+
Sbjct: 81 FKLNLGSKLTVVVNSPSLASEILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYGAEWRM 140
Query: 129 LRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSG--EAPATLGSLLTVCTANVLGRT 188
LRK+ LFS K LD +R +EI + L + G ++P +G L + N++
Sbjct: 141 LRKVCAAKLFSRKTLDSFYELRRKEIRERTRCLYQKGLEKSPVNVGEQLFLTMMNLMMNM 200
Query: 189 MISRRVFGDGSGGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVASEMKKVHK 248
+ V + + FK ++ ++ L G N DFFP L DL G+ +M +
Sbjct: 201 LWGGSVKAEDM--ESVGTEFKGVISEITRLLGVPNVSDFFPMLARFDLQGLVKKMHLYAR 260
Query: 249 WFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDSEIKALLLNMF 308
D L + +E+ + L + GD G +D L L+ +D+ D + + + +KA+L++M
Sbjct: 261 DLDAILDRAIEQMQRLRSRDGDDGECKDFLQHLMKLRDQEADSDVPITMNHVKAVLMDMV 320
Query: 309 TAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYLQAVVKET 368
GT++S +T+E+ + ELI +P++M AQ+ELD VVG+D V + ++ LPY+ AV+KET
Sbjct: 321 VGGTESSTNTIEFVMAELISNPELMRRAQQELDEVVGKDNIVEESHITSLPYILAVLKET 380
Query: 369 FRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFRPNRFLPG 428
RL+P PL VP +E+ + GY IPK + + +++W+I RDP W P EFRP RFL
Sbjct: 381 LRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTEFRPERFL-- 440
Query: 429 GEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPNGLLPDKLN 488
+K D G D+ +PFG+GRRIC G+++ RM+ ATL HSFDW +P+G + L+
Sbjct: 441 -DKKSCDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWTIPDGHV---LD 500
Query: 489 MDEAYGLTLRRAQPLVVHPRPRL 510
++E +G+ L+ PLV P PRL
Sbjct: 501 LEEKFGIVLKLKTPLVALPIPRL 513
BLAST of HG10008538 vs. TAIR 10
Match:
AT1G01280.1 (cytochrome P450, family 703, subfamily A, polypeptide 2 )
HSP 1 Score: 339.0 bits (868), Expect = 6.9e-93
Identity = 192/517 (37.14%), Postives = 285/517 (55.13%), Query Frame = 0
Query: 6 LIVAAVVVTLAYAILLHRAFFLRRRFC----LPPGPKPWPIVGNMPQIGGAPLHSLLAAM 65
L++A++ L +LL R +L+ C LPPGP PI+GN+ Q+G P H LA++
Sbjct: 3 LVLASLFAVLILNVLLWR--WLKASACKAQRLPPGPPRLPILGNLLQLGPLP-HRDLASL 62
Query: 66 AGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFAP 125
+YGPL+YLRLG+VD + ++L F SRP A ++AY D+ AP
Sbjct: 63 CDKYGPLVYLRLGNVDAITTNDPDTIREILLRQDDVFSSRPKTLAAVHLAYGCGDVALAP 122
Query: 126 YGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEA--PATLGSLLTVCT 185
GP W+ +R+I HL ++K L+ R EE L++ + + E P L +L +
Sbjct: 123 MGPHWKRMRRICMEHLLTTKRLESFTTQRAEEARYLIRDVFKRSETGKPINLKEVLGAFS 182
Query: 186 ANVLGRTMISRRVFGDGSGGDP-EANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVA 245
N + R ++ ++ FG GS P EA F + ++ L G GD+ P W+D G
Sbjct: 183 MNNVTRMLLGKQFFGPGSLVSPKEAQEFLHITHKLFWLLGVIYLGDYLPFWRWVDPSGCE 242
Query: 246 SEMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDSEI 305
EM+ V K D+F TKI++EHR D+ GD D + LLS E+ + D EI
Sbjct: 243 KEMRDVEKRVDEFHTKIIDEHRRAKLEDEDKNGDMDFVDVLLSL--PGENGKAHMEDVEI 302
Query: 306 KALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPY 365
KAL+ +M A TDTSA T EWA+ E I+ P++M + Q+ELD VVG +R V + DL L Y
Sbjct: 303 KALIQDMIAAATDTSAVTNEWAMAEAIKQPRVMRKIQEELDNVVGSNRMVDESDLVHLNY 362
Query: 366 LQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEF 425
L+ VV+ETFR+HP P +P + + INGY+IP + + ++ + R+ K W D +F
Sbjct: 363 LRCVVRETFRMHPAGPFLIPHESVRATTINGYYIPAKTRVFINTHGLGRNTKIWDDVEDF 422
Query: 426 RPNRFLPGGEKAHVDVK-GNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALP 485
RP R P V++ G DF+++PF AG+R C G +G+ MV M A L H F+W+ P
Sbjct: 423 RPERHWPVEGSGRVEISHGPDFKILPFSAGKRKCPGAPLGVTMVLMALARLFHCFEWSSP 482
Query: 486 NGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVY 515
++ E YG+T+ +A+PL +PRL +Y
Sbjct: 483 G-----NIDTVEVYGMTMPKAKPLRAIAKPRLAAHLY 509
BLAST of HG10008538 vs. TAIR 10
Match:
AT4G12320.1 (cytochrome P450, family 706, subfamily A, polypeptide 6 )
HSP 1 Score: 337.4 bits (864), Expect = 2.0e-92
Identity = 182/508 (35.83%), Postives = 285/508 (56.10%), Query Frame = 0
Query: 4 ISLIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAAMAG 63
I+L A+++ +A +L F + LPPGP+ PIVGN+P + LH+ +A
Sbjct: 13 INLTPYAILILIAIFSILWYLFKRSPQPHLPPGPRGLPIVGNLPFL-DPDLHTYFTKLAE 72
Query: 64 EYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFAPYG 123
YGP+ L LGS VV + S+A ++LK NF + A+ + Y D+V+ PYG
Sbjct: 73 SYGPIFKLNLGSKLTVVVNTPSLAREILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYG 132
Query: 124 PRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSG--EAPATLGSLLTVCTAN 183
WR+LRK+ + L S + L+ +R +EI + L + G E+P +G + + N
Sbjct: 133 AEWRMLRKVCVLKLLSHRTLNSFYELRRKEIRERTRYLYQKGQEESPVNVGEQVFLTMMN 192
Query: 184 VLGRTMISRRVFGDGSGGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVASEM 243
+ + V + + FK ++ ++ L G+ N DFFP L DL G+ +M
Sbjct: 193 LTMNMLWGGSVKAEEM--ESVGTEFKEVISEITRLLGEPNVSDFFPRLARFDLQGLVKKM 252
Query: 244 KKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDSEIKAL 303
+ D L + +E+ + L GD G +D L L+ KD+ D + + +KA+
Sbjct: 253 HVCARELDAILDRAIEQMQLLRTRDGDDGECKDFLQHLMKLKDQEADSEVPITVNHVKAV 312
Query: 304 LLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYLQA 363
L+++ GTDTS +T+E+A+ ELIR P++M AQ+ELD VVG+D + + +++LP++ A
Sbjct: 313 LVDLVVGGTDTSTNTIEFAMAELIRKPELMKRAQQELDEVVGKDNIIEESHITRLPFISA 372
Query: 364 VVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFRPN 423
++KET RL+P PL VP +E+ + GY IPK + + +++W+I RDP W P EFRP
Sbjct: 373 IMKETLRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTEFRPE 432
Query: 424 RFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPNGLL 483
RFL +K D G D+ +PFG+GRRIC G+++ RM+ ATL HSFDW +P G +
Sbjct: 433 RFL---DKKSCDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWKIPEGHI 492
Query: 484 PDKLNMDEAYGLTLRRAQPLVVHPRPRL 510
L++ E +G+ L+ PLV P PRL
Sbjct: 493 ---LDLKEKFGIVLKLKSPLVALPVPRL 511
BLAST of HG10008538 vs. TAIR 10
Match:
AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )
HSP 1 Score: 333.6 bits (854), Expect = 2.9e-91
Identity = 190/489 (38.85%), Postives = 285/489 (58.28%), Query Frame = 0
Query: 29 RRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAAMAGEYGPLMYLRLGSVDVVVAASASVAE 88
R LPPGP PI+GN+ Q+G P HS A +A YGP+M L+ G ++ VV S A
Sbjct: 34 RAATLPPGPPRLPIIGNIHQVGKNP-HSSFADLAKIYGPIMSLKFGCLNSVVITSPEAAR 93
Query: 89 QLLKIHGTNFLSRPPNAGAKYIAYNNRDMVF-APYGPRWRLLRKISTVHLFSSKALDDHR 148
++L+ H R N + + +++ P RWR+LRK+S +FS + + +
Sbjct: 94 EVLRTHDQILSGRKSNDSIRCFGHEEVSVIWLPPSSARWRMLRKLSVTLMFSPQRTEATK 153
Query: 149 RIREEEIGVLVQALARSGEAPATL----GSLLTVCTANVLGRTMISRRVFGDGSGGDPEA 208
+R +++ LV + S E + S TV N++ + S + GS ++
Sbjct: 154 ALRMKKVQELVSFMNESSERKEAVDISRASYTTV--LNIISNILFSVDL---GSYDSKKS 213
Query: 209 NIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVASEMK----KVHKWFDDFL-TKIVEE 268
N F+ V M GK + ++FP + +LDL G M+ ++ + F F+ KI E+
Sbjct: 214 NEFQDTVIGAMEAAGKPDAANYFPFMGFLDLQGNRKAMRGLTERLFRVFRGFMDAKIAEK 273
Query: 269 HRNLLAGGGDRGGDQDLLSTL--LSSKDEAEDENEKLNDSEIKALLLNMFTAGTDTSAST 328
L ++D L +L L+ DEAE L++++I+ LLL+MFTAGTDTS+ST
Sbjct: 274 S---LGNYSKDVSNRDFLDSLLILNEGDEAE-----LDNNDIEHLLLDMFTAGTDTSSST 333
Query: 329 VEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYLQAVVKETFRLHPPTPLS 388
+EWA+ EL+R+PK M +AQ E+D V+G++ V + D+S LPYLQAVVKETFRLHP PL
Sbjct: 334 LEWAMAELLRNPKTMVKAQAEMDRVLGQNSVVQESDISGLPYLQAVVKETFRLHPAAPLL 393
Query: 389 VPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFRPNRFLPGGEKAHVDVKG 448
VPR A E+ G+ +PK + +LV++WAI RDP W +P +F P RF+ +DVKG
Sbjct: 394 VPRKAESDVEVLGFMVPKDTQVLVNVWAIGRDPSVWENPSQFEPERFM----GKDIDVKG 453
Query: 449 NDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPNGLLPDKLNMDEAYGLTLR 506
D+E+ PFG GRRIC G+ + ++ V ++ A+L +SFDW LPNG++ + L+MDE +G+TL
Sbjct: 454 RDYELTPFGGGRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVSEDLDMDETFGITLH 504
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_038878698.1 | 7.8e-264 | 89.79 | flavonoid 3'-monooxygenase [Benincasa hispida] | [more] |
XP_008453263.1 | 5.8e-259 | 86.59 | PREDICTED: flavonoid 3'-monooxygenase [Cucumis melo] >KAA0057987.1 flavonoid 3'-... | [more] |
XP_004138192.1 | 1.9e-254 | 85.30 | flavonoid 3'-monooxygenase [Cucumis sativus] >KGN63725.2 hypothetical protein Cs... | [more] |
XP_023529494.1 | 9.3e-249 | 83.11 | flavonoid 3'-monooxygenase [Cucurbita pepo subsp. pepo] >XP_023529495.1 flavonoi... | [more] |
XP_022933611.1 | 1.7e-247 | 83.24 | flavonoid 3'-monooxygenase [Cucurbita moschata] | [more] |
Match Name | E-value | Identity | Description | |
Q9SBQ9 | 1.0e-189 | 63.57 | Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1 | [more] |
Q9SD85 | 1.8e-183 | 61.75 | Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP75B1 PE=1 SV=1 | [more] |
A0A4D6Q415 | 1.3e-165 | 56.78 | Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN... | [more] |
Q7G602 | 6.2e-163 | 57.61 | Flavonoid 3'-monooxygenase CYP75B3 OS=Oryza sativa subsp. japonica OX=39947 GN=C... | [more] |
Q8LM92 | 1.6e-158 | 54.60 | Flavonoid 3'-monooxygenase CYP75B4 OS=Oryza sativa subsp. japonica OX=39947 GN=C... | [more] |
Match Name | E-value | Identity | Description | |
A0A5A7UTD5 | 2.8e-259 | 86.59 | Flavonoid 3'-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... | [more] |
A0A1S3BVU8 | 2.8e-259 | 86.59 | flavonoid 3'-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103494036 PE=3 SV=1 | [more] |
A0A6J1EZJ7 | 8.5e-248 | 83.24 | flavonoid 3'-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111440987 PE=3 SV... | [more] |
A0A6J1L0D6 | 3.6e-246 | 82.66 | flavonoid 3'-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111499893 PE=3 SV=1 | [more] |
A0A0A0LP99 | 2.8e-227 | 79.53 | Flavonoid 3' hydroxylase OS=Cucumis sativus OX=3659 GN=Csa_1G013190 PE=3 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
AT5G07990.1 | 1.3e-184 | 61.75 | Cytochrome P450 superfamily protein | [more] |
AT4G12310.1 | 1.8e-93 | 36.38 | cytochrome P450, family 706, subfamily A, polypeptide 5 | [more] |
AT1G01280.1 | 6.9e-93 | 37.14 | cytochrome P450, family 703, subfamily A, polypeptide 2 | [more] |
AT4G12320.1 | 2.0e-92 | 35.83 | cytochrome P450, family 706, subfamily A, polypeptide 6 | [more] |
AT2G45550.1 | 2.9e-91 | 38.85 | cytochrome P450, family 76, subfamily C, polypeptide 4 | [more] |