HG10008227 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10008227
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	tRNA (Adenine(37)-N6)-methyltransferase
Location	Chr10: 21051309 .. 21053815 (+)
RNA-Seq Expression	HG10008227
Synteny	HG10008227

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTACGCCGCGGAGTCAAGATGGTCGACGGCCGCCATGAGCGTCACACTGGCGTTTATCTCCGCCTCCGCCGTCATATCTCGTAAAACTTCGAAACTTTAAAGATCCTTTATGGCCTCTCTCTAAATTTATCTGATTTCCTATTTGTTTCTTACAATGGAAGTTTTGTGGTTTTCCCCAGTTTATCTTGTGACAAAGAAGTCGAGAGAGTTGGGTACCAAGGTTCGAGAGCTCGAGGCTTCTCTGGCAGCTTCTTTAAAGAAAACCGCCGCCGAGAGACAAGGCCGAATACGGGCACAGCAGGTATTTCATATTTTCTCCTCTGCATCGCTTCATGTTCCAGTGTTGTTATTGATATTTTGGTTTCATTTTTTCTCCCTATTTGTAGGCTTTAAGGAAAGCAGTTGCGGAACCGAAGTCTGATAGCCTGGAGTGTACTTCATATCCCATGACACCAATTGGTACTATACGATCATCATTCTCTACTAGGTTAGTTTTCGATTGCATTATCTGGTAGTCCAAACATCAAATATTGGGTTGTTGTTTTCCCATTGTTCTGTTCACAAAACTAATTTCAGAAGTAGATATGTTTGAGCCATATTCTTCAGTTAGCTGTAGAAATCGAGCGTTCAACAACTTTTTTGGATTTTCTTTTTAGGAATGGGACTCCAAGGCAATCTTTACTCGTACCTCTTGCAAGGGCACGGTTGGTATTTTCTGCTCGCATTCCTCCAGCTTCCATTGAAGGTCTTGGAGAATATTCCCATTGCTGGGTCATATATGTCTTTCATTTAAACACAGATCTTGACAAGTTGTGGAAGGATCCATCGAAATCAAAATTCAAGGCAAAAGTATGTTTTCTTCATTTGTACTTAAATATGGAACTTATATTTTTGTTACTCTTATAATTTCCTAAAATCCCATAAAAGGACAGCTCAATGGTAGGTTAGAGTACCGAGGTTGAAAGGCGAAAGAGTAGGAGTCTTTGCTACAAGATCTCCACATAGACCGTGCCCAATCGGACTCACCATTGCAAAGGTAAGATAAAAATTTCAACTGCTGTCAATGTATCTCACAAGTTTATGGAGAAGCCCATCATAGATCAAAGACATTTATGAAATATGTGAAGCAAACGAGTTTTAGAGAGGTTTTGCTGTCACATGAACATGGTCCAATACGTTTTGTATGGTTAGGCAGGCTGTATCAATGTCATTTAGTATTAGAAATTCATGGTGACAAGCTTACAGTTGCAAGTTTAGACAACATTCAAATGCAATAGTTAAATGGGGGAATATTAAATCTAAGATTAATGAATCTGCTATTTTACCACTTCAGGTGGAGGCTATCCAGGGACATCAAATTCTACTCTCTGGTGCTGATCTTGTTGATGGAACTGTAAGAGTTTGTTTTCACTTTTTCCAACCATCGATAAATGATTTATCTTGAATTTAGTTCTTATTGTGGTAATGATTGTTATGATTTCTTGCTTTGTTTGAACTTTGTTACAGCCAGTACTTGATGTTAAACCTTATCTACCGTACTGTGACAGTGTCCAAGATGCAACAGTCCCAAAGTGGTTACTGGTATGTGTTCAATAAATAATTTCTATGACTAAACGTTATTTTAGAATTCCTTTGATGCTTATCTTGTTACTATTCATCTTTCAACTGATTTTTTATGTGGTAATTTTTAGCAATCATTCTCTCATTCGTTTGTCCCAATGGAGATTTATTCCGAGTGTTGTGCTTTTCACACTTCAAAATGTAGAAATTCTCTTCTCATATGCATGCCATATGTGATATGACTAGTTGCGTGCAAATAACTTAAGAAATTCAGCTTTCGATATACTATTTGGTGCTATTGCTACTTTCATAGTTGGAGGCATCGATACTCTATGACCATGTAATATGTTTATATACATTCCTCTTGGCATTCTTACATCTTATTTACACTAACACAGGGGGACAATTTATTGACTGTTGCATCCATTTGCTTCTCTGAAGAATTTATTACAACTCTATCCGAGTGTTGGCCACTAATGGTGAGTTCCTTCTTTTTTCCTTATAATTAATTAATCATTGTTGTTATTATTATTATTATTACATATGTATGTATGGGACGTCCTTGCTGGAGTTTTGGGTGATAATCTTATCATATTTTTCTTGTTTCAGGAAAAGAAATCGCTTTATGCATCAGAAGATGAGTTAAAAAACTTTGTGAAGCAGGTGCTTTCTTGGGATATTCGATCAGTGTCTCAACGTAATCGACCTCATGACTCCCACGTTAAGACAACAGCAGGACATGAATTGTCCGATGGTAGTTCAGATTCAGATGGTCACCTGGATGACAAAGTTTCGAGTGGTTCCCAAAGTTCTTGTAAGAGCACTATCTATCACCTACTTGTAGATGAATTAGATGTGTCGTACACAATTGGTTGTGATGGGGACGTGGTTGTGGGCAAAGTGGAACTTGCATCACCCAGCTCAAGAACAAGATGCAATTAA

mRNA sequence

ATGGCCTACGCCGCGGAGTCAAGATGGTCGACGGCCGCCATGAGCGTCACACTGGCGTTTATCTCCGCCTCCGCCGTCATATCTCTTTATCTTGTGACAAAGAAGTCGAGAGAGTTGGGTACCAAGGTTCGAGAGCTCGAGGCTTCTCTGGCAGCTTCTTTAAAGAAAACCGCCGCCGAGAGACAAGGCCGAATACGGGCACAGCAGGCTTTAAGGAAAGCAGTTGCGGAACCGAAGTCTGATAGCCTGGAGTGTACTTCATATCCCATGACACCAATTGGTACTATACGATCATCATTCTCTACTAGGAATGGGACTCCAAGGCAATCTTTACTCGTACCTCTTGCAAGGGCACGGTTGGTATTTTCTGCTCGCATTCCTCCAGCTTCCATTGAAGGTCTTGGAGAATATTCCCATTGCTGGGTCATATATGTCTTTCATTTAAACACAGATCTTGACAAGTTGTGGAAGGATCCATCGAAATCAAAATTCAAGGCAAAAGTTAGAGTACCGAGGTTGAAAGGCGAAAGAGTAGGAGTCTTTGCTACAAGATCTCCACATAGACCGTGCCCAATCGGACTCACCATTGCAAAGGTGGAGGCTATCCAGGGACATCAAATTCTACTCTCTGGTGCTGATCTTGTTGATGGAACTCCAGTACTTGATGTTAAACCTTATCTACCGTACTGTGACAGTGTCCAAGATGCAACAGTCCCAAAGTGGTTACTGGGGGACAATTTATTGACTGTTGCATCCATTTGCTTCTCTGAAGAATTTATTACAACTCTATCCGAGTGTTGGCCACTAATGGAAAAGAAATCGCTTTATGCATCAGAAGATGAGTTAAAAAACTTTGTGAAGCAGGTGCTTTCTTGGGATATTCGATCAGTGTCTCAACGTAATCGACCTCATGACTCCCACGTTAAGACAACAGCAGGACATGAATTGTCCGATGGTAGTTCAGATTCAGATGGTCACCTGGATGACAAAGTTTCGAGTGGTTCCCAAAGTTCTTGTAAGAGCACTATCTATCACCTACTTGTAGATGAATTAGATGTGTCGTACACAATTGGTTGTGATGGGGACGTGGTTGTGGGCAAAGTGGAACTTGCATCACCCAGCTCAAGAACAAGATGCAATTAA

Coding sequence (CDS)

ATGGCCTACGCCGCGGAGTCAAGATGGTCGACGGCCGCCATGAGCGTCACACTGGCGTTTATCTCCGCCTCCGCCGTCATATCTCTTTATCTTGTGACAAAGAAGTCGAGAGAGTTGGGTACCAAGGTTCGAGAGCTCGAGGCTTCTCTGGCAGCTTCTTTAAAGAAAACCGCCGCCGAGAGACAAGGCCGAATACGGGCACAGCAGGCTTTAAGGAAAGCAGTTGCGGAACCGAAGTCTGATAGCCTGGAGTGTACTTCATATCCCATGACACCAATTGGTACTATACGATCATCATTCTCTACTAGGAATGGGACTCCAAGGCAATCTTTACTCGTACCTCTTGCAAGGGCACGGTTGGTATTTTCTGCTCGCATTCCTCCAGCTTCCATTGAAGGTCTTGGAGAATATTCCCATTGCTGGGTCATATATGTCTTTCATTTAAACACAGATCTTGACAAGTTGTGGAAGGATCCATCGAAATCAAAATTCAAGGCAAAAGTTAGAGTACCGAGGTTGAAAGGCGAAAGAGTAGGAGTCTTTGCTACAAGATCTCCACATAGACCGTGCCCAATCGGACTCACCATTGCAAAGGTGGAGGCTATCCAGGGACATCAAATTCTACTCTCTGGTGCTGATCTTGTTGATGGAACTCCAGTACTTGATGTTAAACCTTATCTACCGTACTGTGACAGTGTCCAAGATGCAACAGTCCCAAAGTGGTTACTGGGGGACAATTTATTGACTGTTGCATCCATTTGCTTCTCTGAAGAATTTATTACAACTCTATCCGAGTGTTGGCCACTAATGGAAAAGAAATCGCTTTATGCATCAGAAGATGAGTTAAAAAACTTTGTGAAGCAGGTGCTTTCTTGGGATATTCGATCAGTGTCTCAACGTAATCGACCTCATGACTCCCACGTTAAGACAACAGCAGGACATGAATTGTCCGATGGTAGTTCAGATTCAGATGGTCACCTGGATGACAAAGTTTCGAGTGGTTCCCAAAGTTCTTGTAAGAGCACTATCTATCACCTACTTGTAGATGAATTAGATGTGTCGTACACAATTGGTTGTGATGGGGACGTGGTTGTGGGCAAAGTGGAACTTGCATCACCCAGCTCAAGAACAAGATGCAATTAA

Protein sequence

MAYAAESRWSTAAMSVTLAFISASAVISLYLVTKKSRELGTKVRELEASLAASLKKTAAERQGRIRAQQALRKAVAEPKSDSLECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARLVFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGVFATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPKWLLGDNLLTVASICFSEEFITTLSECWPLMEKKSLYASEDELKNFVKQVLSWDIRSVSQRNRPHDSHVKTTAGHELSDGSSDSDGHLDDKVSSGSQSSCKSTIYHLLVDELDVSYTIGCDGDVVVGKVELASPSSRTRCN

Homology

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BLAST of HG10008227 vs. NCBI nr
Match: XP_038879626.1 (tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Benincasa hispida])

HSP 1 Score: 699.5 bits (1804), Expect = 1.6e-197
Identity = 357/380 (93.95%), Postives = 368/380 (96.84%), Query Frame = 0


Query: 1   MAYAAESRWSTAAMSVTLAFISASAVISLYLVTKKSRELGTKVRELEASLAASLKKTAAE 60
           MAYAAESRWS AA+SVTLAFISASAVISLY+VTKKSRELGTKVRELEASLA+SLKKTAAE
Sbjct: 1   MAYAAESRWSMAALSVTLAFISASAVISLYVVTKKSRELGTKVRELEASLASSLKKTAAE 60

Query: 61  RQGRIRAQQALRKAVAEPKSDSLECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARL 120
           RQGRIRAQQALRKAVAEPKSDS+ECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARL 120

Query: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180
           VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV
Sbjct: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240
           FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK
Sbjct: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240

Query: 241 WLLGDNLLTVASICFSEEFITTLSECWPLMEKKSLYASEDELKNFVKQVLSWDIRSVSQR 300
           WLLGDNLLTVASICFSEEFI TLSECWPLMEKKSLYASEDE +NFVK+VLSWDIRSVSQR
Sbjct: 241 WLLGDNLLTVASICFSEEFIPTLSECWPLMEKKSLYASEDEFRNFVKEVLSWDIRSVSQR 300

Query: 301 NRPHDSHVKTTAGHELSDGSSDSDGHLDDKVSSGSQSSCKSTIYHLLVDELDVSYTIGCD 360
           N+PHDSHVK T GHE  DGSSDS+GHLDDKVSSGSQSS ++ IYHLLVD LDVSYTIGC 
Sbjct: 301 NQPHDSHVKPTTGHECFDGSSDSEGHLDDKVSSGSQSSSENIIYHLLVDGLDVSYTIGCH 360

Query: 361 GDVVVGKVELASPSSRTRCN 381
           G+VVVGKVELASPSSRTR N
Sbjct: 361 GNVVVGKVELASPSSRTRSN 380

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BLAST of HG10008227 vs. NCBI nr
Match: TYK09463.1 (tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 672.2 bits (1733), Expect = 2.7e-189
Identity = 344/380 (90.53%), Postives = 361/380 (95.00%), Query Frame = 0


Query: 1   MAYAAESRWSTAAMSVTLAFISASAVISLYLVTKKSRELGTKVRELEASLAASLKKTAAE 60
           MAYAAES WSTAAMSV L FISASA+ISLYLVTKKSRELGTKV++LEASLAASLKKTAAE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 61  RQGRIRAQQALRKAVAEPKSDSLECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARL 120
           RQGRIRAQQALRKAVAEPKSDS+ECTSYPMTPIGTI+SSF+TRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180
           VFSARIPPASIEGL EYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240
           FATRSPHRPCPIGLT+AKVEA+QGH+ILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK
Sbjct: 181 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240

Query: 241 WLLGDNLLTVASICFSEEFITTLSECWPLMEKKSLYASEDELKNFVKQVLSWDIRSVSQR 300
           WLLGDN+LTVASICFSEEF + LS+CWPL+EKKSLYASE+ELKNFVKQVLSWDIRSVSQR
Sbjct: 241 WLLGDNVLTVASICFSEEFTSALSKCWPLVEKKSLYASEEELKNFVKQVLSWDIRSVSQR 300

Query: 301 NRPHDSHVKTTAGHELSDGSSDSDGHLDDKVSSGSQSSCKSTIYHLLVDELDVSYTIGCD 360
           +RPH SH+KT  G EL D SSDSDGH+DDKVSS SQSS KSTIYHLLVD LDVSYTIG  
Sbjct: 301 SRPHCSHIKTVTGDELLDESSDSDGHVDDKVSSSSQSSSKSTIYHLLVDGLDVSYTIGYH 360

Query: 361 GDVVVGKVELASPSSRTRCN 381
           GDVVV KVELASPSSRTR N
Sbjct: 361 GDVVVDKVELASPSSRTRSN 380

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BLAST of HG10008227 vs. NCBI nr
Match: XP_004149885.1 (uncharacterized protein LOC101219357 [Cucumis sativus] >KGN65285.1 hypothetical protein Csa_019626 [Cucumis sativus])

HSP 1 Score: 671.4 bits (1731), Expect = 4.6e-189
Identity = 345/380 (90.79%), Postives = 362/380 (95.26%), Query Frame = 0


Query: 1   MAYAAESRWSTAAMSVTLAFISASAVISLYLVTKKSRELGTKVRELEASLAASLKKTAAE 60
           M YAA+SRWSTAAMSVTLAFISASA+ISLYLVTKKSRELGTKVR+LEASLAASLKKTAAE
Sbjct: 1   MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 60

Query: 61  RQGRIRAQQALRKAVAEPKSDSLECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARL 120
           RQGRIRAQQALRKAVAEPKSDS++CTSYPMTPIGTI+SSF+TRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180
           VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV
Sbjct: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240
           FATRSPHRPCPIGLT+AKVEA+QGH+ILLSGADLVDGTPVLDVKPYLPYCDSVQ+ATVPK
Sbjct: 181 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGTPVLDVKPYLPYCDSVQNATVPK 240

Query: 241 WLLGDNLLTVASICFSEEFITTLSECWPLMEKKSLYASEDELKNFVKQVLSWDIRSVSQR 300
           WLLGDN+LTVASICFSEEF +TLS+CWPL EKKSLYASEDELKNFVKQVLSWDIRSVSQR
Sbjct: 241 WLLGDNVLTVASICFSEEFTSTLSKCWPLTEKKSLYASEDELKNFVKQVLSWDIRSVSQR 300

Query: 301 NRPHDSHVKTTAGHELSDGSSDSDGHLDDKVSSGSQSSCKSTIYHLLVDELDVSYTIGCD 360
           +RPH SH+KT      SD SSDSDGHL+DKVSS SQSS KSTIYHLLVD LDVSYTIGC 
Sbjct: 301 SRPHCSHIKTD-----SDDSSDSDGHLNDKVSSSSQSSSKSTIYHLLVDGLDVSYTIGCH 360

Query: 361 GDVVVGKVELASPSSRTRCN 381
           GDVVV KVELAS SSRTR N
Sbjct: 361 GDVVVDKVELASSSSRTRSN 375

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BLAST of HG10008227 vs. NCBI nr
Match: XP_008453657.1 (PREDICTED: tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Cucumis melo])

HSP 1 Score: 667.9 bits (1722), Expect = 5.1e-188
Identity = 342/380 (90.00%), Postives = 360/380 (94.74%), Query Frame = 0


Query: 1   MAYAAESRWSTAAMSVTLAFISASAVISLYLVTKKSRELGTKVRELEASLAASLKKTAAE 60
           MAYAAES WSTAAMSV L FISASA+ISLYLVTKKSRELGTKV++LEASLAASLKKTAAE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 61  RQGRIRAQQALRKAVAEPKSDSLECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARL 120
           RQGRIRAQQALRKAVAEPKSDS+ECTSYPMTPIGTI+SSF+TRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180
           VFSARIPPASIEGL EYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240
           FATRSPHRPCPIGLT+AKVEA+QGH+ILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK
Sbjct: 181 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240

Query: 241 WLLGDNLLTVASICFSEEFITTLSECWPLMEKKSLYASEDELKNFVKQVLSWDIRSVSQR 300
           WLLGD++LTVASICFSEEF + LS+CWPL+EKKSLYASE+ELKNFVKQVLSWDIRSVSQR
Sbjct: 241 WLLGDSVLTVASICFSEEFTSALSKCWPLVEKKSLYASEEELKNFVKQVLSWDIRSVSQR 300

Query: 301 NRPHDSHVKTTAGHELSDGSSDSDGHLDDKVSSGSQSSCKSTIYHLLVDELDVSYTIGCD 360
           +RPH SH+KT  G EL D SSDSDGH+DDKVSS SQS  KSTIYHLLVD LDVSYTIG  
Sbjct: 301 SRPHCSHIKTVTGDELLDESSDSDGHVDDKVSSSSQSYSKSTIYHLLVDGLDVSYTIGYH 360

Query: 361 GDVVVGKVELASPSSRTRCN 381
           GDVVV KVELASPSSRTR N
Sbjct: 361 GDVVVDKVELASPSSRTRSN 380

[Close]

BLAST of HG10008227 vs. NCBI nr
Match: XP_022134721.1 (uncharacterized protein LOC111006924 [Momordica charantia])

HSP 1 Score: 656.8 bits (1693), Expect = 1.2e-184
Identity = 329/380 (86.58%), Postives = 357/380 (93.95%), Query Frame = 0


Query: 1   MAYAAESRWSTAAMSVTLAFISASAVISLYLVTKKSRELGTKVRELEASLAASLKKTAAE 60
           MAY AESRWSTAAMS+ LAFISASA++SLYLVT+KSRELG+KVRELEASLAASLKKT AE
Sbjct: 1   MAYGAESRWSTAAMSIILAFISASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTTAE 60

Query: 61  RQGRIRAQQALRKAVAEPKSDSLECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARL 120
           RQGRIRAQQALRKA+A+PKSD++ECTSYPMTPIG I+S+FSTRNGTPRQSL+VPLARARL
Sbjct: 61  RQGRIRAQQALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARL 120

Query: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180
           VFSARIPPASIEGLGEYSHCW+IYVFHLNTDLDKLWK+PSKS+FKAKVRVPRLKG+RVGV
Sbjct: 121 VFSARIPPASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKRVGV 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240
           FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVP+
Sbjct: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPE 240

Query: 241 WLLGDNLLTVASICFSEEFITTLSECWPLMEKKSLYASEDELKNFVKQVLSWDIRSVSQR 300
           WL+GDNLL+VASICFSEEF +TLS+CWPLMEKKS YAS DE KNFVKQVLSWDIRS+SQR
Sbjct: 241 WLMGDNLLSVASICFSEEFTSTLSDCWPLMEKKSHYASADEFKNFVKQVLSWDIRSLSQR 300

Query: 301 NRPHDSHVKTTAGHELSDGSSDSDGHLDDKVSSGSQSSCKSTIYHLLVDELDVSYTIGCD 360
            RPH+SH++TT GHELSDGSSD D HL DK S G+Q S K+ IYHLLVD L+VSYTIGC 
Sbjct: 301 TRPHESHIETTTGHELSDGSSDLDTHLYDKASGGTQISSKNIIYHLLVDGLNVSYTIGCH 360

Query: 361 GDVVVGKVELASPSSRTRCN 381
           GDVVV K ELASPSSRTR N
Sbjct: 361 GDVVVVKAELASPSSRTRSN 380

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BLAST of HG10008227 vs. ExPASy Swiss-Prot
Match: Q9BU70 (tRNA (adenine(37)-N6)-methyltransferase OS=Homo sapiens OX=9606 GN=TRMO PE=1 SV=2)

HSP 1 Score: 140.6 bits (353), Expect = 3.7e-32
Identity = 74/161 (45.96%), Postives = 99/161 (61.49%), Query Frame = 0


Query: 82  SLECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARLVFSARI---PPASIEGLGEYS 141
           +LE  +    P+G + S FS +NGTPRQ  +   +RA L    RI   P  S+ GL ++S
Sbjct: 22  ALETGNLLTEPVGYLESCFSAKNGTPRQPSICSYSRACLRIRKRIFNNPEHSLMGLEQFS 81

Query: 142 HCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGVFATRSPHRPCPIGLTIAK 201
           H W+++VFH N  L            KAKV+ PRL G + GVF+TRSPHRP  IGLT+AK
Sbjct: 82  HVWILFVFHKNGHL----------SCKAKVQPPRLNGAKTGVFSTRSPHRPNAIGLTLAK 141

Query: 202 VEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVP 240
           +E ++G  I LSG D++ GTPVLD+KPY+   DS Q+   P
Sbjct: 142 LEKVEGGAIYLSGIDMIHGTPVLDIKPYIAEYDSPQNVMEP 172

[Close]

BLAST of HG10008227 vs. ExPASy Swiss-Prot
Match: Q562D6 (tRNA (adenine(37)-N6)-methyltransferase OS=Mus musculus OX=10090 GN=Trmo PE=2 SV=2)

HSP 1 Score: 139.8 bits (351), Expect = 6.3e-32
Identity = 73/160 (45.62%), Postives = 100/160 (62.50%), Query Frame = 0


Query: 82  SLECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARLVFSARI---PPASIEGLGEYS 141
           +LE  +    PIG + S F  + GTPRQ  +   +RA L     I   P  S+ GL E+S
Sbjct: 22  ALEAGNLLTEPIGYLESCFPAKIGTPRQPSICSHSRACLKIRKNIFNNPEHSLMGLEEFS 81

Query: 142 HCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGVFATRSPHRPCPIGLTIAK 201
           H W+++VFH N  L+          +KAKV+ PRL G + GVF+TRSPHRP  IGLT+AK
Sbjct: 82  HVWILFVFHKNGHLN----------YKAKVQPPRLNGAKTGVFSTRSPHRPNAIGLTLAK 141

Query: 202 VEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATV 239
           +E ++G  + LSG D++DGTPVLD+KPY+   DS Q+ +V
Sbjct: 142 LEKVEGGAVYLSGVDMIDGTPVLDIKPYIADYDSPQNLSV 171

[Close]

BLAST of HG10008227 vs. ExPASy Swiss-Prot
Match: Q4V7E0 (tRNA (adenine(37)-N6)-methyltransferase OS=Rattus norvegicus OX=10116 GN=Trmo PE=2 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.2e-30
Identity = 71/157 (45.22%), Postives = 98/157 (62.42%), Query Frame = 0


Query: 82  SLECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARLVFSARI---PPASIEGLGEYS 141
           +LE  +    PIG + S FS + GTPRQ  +   +RA L     I   P  S+ GL ++S
Sbjct: 22  ALETGNLLTEPIGYLESCFSAKIGTPRQPSICSQSRACLKIRKSIFNNPEHSLMGLEQFS 81

Query: 142 HCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGVFATRSPHRPCPIGLTIAK 201
           H W+++VFH N  L+          +KAKV+ PRL G + GVF+TRSPHRP  IGLT+AK
Sbjct: 82  HVWILFVFHKNGHLN----------YKAKVQPPRLNGAKTGVFSTRSPHRPNAIGLTLAK 141

Query: 202 VEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQD 236
           +E ++G  + LSG D++ GTPVLD+KPY+   DS Q+
Sbjct: 142 LEKVEGGAVYLSGIDMIHGTPVLDIKPYIADYDSPQN 168

[Close]

BLAST of HG10008227 vs. ExPASy Swiss-Prot
Match: Q9RPT0 (Putative S-adenosylmethionine-dependent methyltransferase RcsF OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=rcsF PE=4 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 9.5e-28
Identity = 68/155 (43.87%), Postives = 96/155 (61.94%), Query Frame = 0


Query: 87  SYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARLVFSARIPP----ASIEGLGEYSHCWV 146
           ++ ++PIG IRS F  +   PRQ LL P AR  L     +PP     ++EGL + SH W+
Sbjct: 2   THSVSPIGYIRSCFMEKFAIPRQPLLAPAARGTLEL---LPPFDQVEALEGLEQVSHVWL 61

Query: 147 IYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGER-VGVFATRSPHRPCPIGLTIAKVEA 206
           +++FH             + K + KVR PRL G R +GVFATR+ HRP  IG ++ ++E 
Sbjct: 62  LFLFH----------QALEDKPRLKVRPPRLGGNRSLGVFATRATHRPNGIGQSVVRLEG 121

Query: 207 IQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDA 237
            +  ++ LSG DL+DGTPVLD+KPY+PY D+V DA
Sbjct: 122 FEAGRLWLSGIDLLDGTPVLDIKPYVPYADAVADA 143

[Close]

BLAST of HG10008227 vs. ExPASy Swiss-Prot
Match: O68638 (Putative S-adenosylmethionine-dependent methyltransferase RcsF OS=Pseudomonas aeruginosa OX=287 GN=rcsF PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 8.8e-26
Identity = 65/155 (41.94%), Postives = 94/155 (60.65%), Query Frame = 0


Query: 87  SYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARLVFSARIPP----ASIEGLGEYSHCWV 146
           S+ ++PIG +RS F  +   PRQ  L P A   L     +PP     ++EGL + SH W+
Sbjct: 2   SHSISPIGHVRSCFKEKFAIPRQPQLAPAATGVLEL---LPPFDTGDAVEGLEQVSHVWL 61

Query: 147 IYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKG-ERVGVFATRSPHRPCPIGLTIAKVEA 206
           I++FH             + K + KVR PRL G + +GVFATR+ HRP  +G ++ K+E 
Sbjct: 62  IFLFH----------QALEDKPRLKVRPPRLGGNQSMGVFATRATHRPNGLGQSVVKLEK 121

Query: 207 IQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDA 237
           ++  ++ LSG DL+DGTPV+D+KPY+PY D V DA
Sbjct: 122 VEPGRLWLSGIDLLDGTPVIDIKPYVPYADIVPDA 143

[Close]

BLAST of HG10008227 vs. ExPASy TrEMBL
Match: A0A5D3CC84 (tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold499G00580 PE=4 SV=1)

HSP 1 Score: 672.2 bits (1733), Expect = 1.3e-189
Identity = 344/380 (90.53%), Postives = 361/380 (95.00%), Query Frame = 0


Query: 1   MAYAAESRWSTAAMSVTLAFISASAVISLYLVTKKSRELGTKVRELEASLAASLKKTAAE 60
           MAYAAES WSTAAMSV L FISASA+ISLYLVTKKSRELGTKV++LEASLAASLKKTAAE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 61  RQGRIRAQQALRKAVAEPKSDSLECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARL 120
           RQGRIRAQQALRKAVAEPKSDS+ECTSYPMTPIGTI+SSF+TRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180
           VFSARIPPASIEGL EYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240
           FATRSPHRPCPIGLT+AKVEA+QGH+ILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK
Sbjct: 181 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240

Query: 241 WLLGDNLLTVASICFSEEFITTLSECWPLMEKKSLYASEDELKNFVKQVLSWDIRSVSQR 300
           WLLGDN+LTVASICFSEEF + LS+CWPL+EKKSLYASE+ELKNFVKQVLSWDIRSVSQR
Sbjct: 241 WLLGDNVLTVASICFSEEFTSALSKCWPLVEKKSLYASEEELKNFVKQVLSWDIRSVSQR 300

Query: 301 NRPHDSHVKTTAGHELSDGSSDSDGHLDDKVSSGSQSSCKSTIYHLLVDELDVSYTIGCD 360
           +RPH SH+KT  G EL D SSDSDGH+DDKVSS SQSS KSTIYHLLVD LDVSYTIG  
Sbjct: 301 SRPHCSHIKTVTGDELLDESSDSDGHVDDKVSSSSQSSSKSTIYHLLVDGLDVSYTIGYH 360

Query: 361 GDVVVGKVELASPSSRTRCN 381
           GDVVV KVELASPSSRTR N
Sbjct: 361 GDVVVDKVELASPSSRTRSN 380

[Close]

BLAST of HG10008227 vs. ExPASy TrEMBL
Match: A0A0A0LTM6 (TsaA-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G295140 PE=4 SV=1)

HSP 1 Score: 671.4 bits (1731), Expect = 2.2e-189
Identity = 345/380 (90.79%), Postives = 362/380 (95.26%), Query Frame = 0


Query: 1   MAYAAESRWSTAAMSVTLAFISASAVISLYLVTKKSRELGTKVRELEASLAASLKKTAAE 60
           M YAA+SRWSTAAMSVTLAFISASA+ISLYLVTKKSRELGTKVR+LEASLAASLKKTAAE
Sbjct: 1   MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 60

Query: 61  RQGRIRAQQALRKAVAEPKSDSLECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARL 120
           RQGRIRAQQALRKAVAEPKSDS++CTSYPMTPIGTI+SSF+TRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180
           VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV
Sbjct: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240
           FATRSPHRPCPIGLT+AKVEA+QGH+ILLSGADLVDGTPVLDVKPYLPYCDSVQ+ATVPK
Sbjct: 181 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGTPVLDVKPYLPYCDSVQNATVPK 240

Query: 241 WLLGDNLLTVASICFSEEFITTLSECWPLMEKKSLYASEDELKNFVKQVLSWDIRSVSQR 300
           WLLGDN+LTVASICFSEEF +TLS+CWPL EKKSLYASEDELKNFVKQVLSWDIRSVSQR
Sbjct: 241 WLLGDNVLTVASICFSEEFTSTLSKCWPLTEKKSLYASEDELKNFVKQVLSWDIRSVSQR 300

Query: 301 NRPHDSHVKTTAGHELSDGSSDSDGHLDDKVSSGSQSSCKSTIYHLLVDELDVSYTIGCD 360
           +RPH SH+KT      SD SSDSDGHL+DKVSS SQSS KSTIYHLLVD LDVSYTIGC 
Sbjct: 301 SRPHCSHIKTD-----SDDSSDSDGHLNDKVSSSSQSSSKSTIYHLLVDGLDVSYTIGCH 360

Query: 361 GDVVVGKVELASPSSRTRCN 381
           GDVVV KVELAS SSRTR N
Sbjct: 361 GDVVVDKVELASSSSRTRSN 375

[Close]

BLAST of HG10008227 vs. ExPASy TrEMBL
Match: A0A1S3BW92 (tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494312 PE=4 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 2.5e-188
Identity = 342/380 (90.00%), Postives = 360/380 (94.74%), Query Frame = 0


Query: 1   MAYAAESRWSTAAMSVTLAFISASAVISLYLVTKKSRELGTKVRELEASLAASLKKTAAE 60
           MAYAAES WSTAAMSV L FISASA+ISLYLVTKKSRELGTKV++LEASLAASLKKTAAE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 61  RQGRIRAQQALRKAVAEPKSDSLECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARL 120
           RQGRIRAQQALRKAVAEPKSDS+ECTSYPMTPIGTI+SSF+TRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180
           VFSARIPPASIEGL EYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240
           FATRSPHRPCPIGLT+AKVEA+QGH+ILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK
Sbjct: 181 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240

Query: 241 WLLGDNLLTVASICFSEEFITTLSECWPLMEKKSLYASEDELKNFVKQVLSWDIRSVSQR 300
           WLLGD++LTVASICFSEEF + LS+CWPL+EKKSLYASE+ELKNFVKQVLSWDIRSVSQR
Sbjct: 241 WLLGDSVLTVASICFSEEFTSALSKCWPLVEKKSLYASEEELKNFVKQVLSWDIRSVSQR 300

Query: 301 NRPHDSHVKTTAGHELSDGSSDSDGHLDDKVSSGSQSSCKSTIYHLLVDELDVSYTIGCD 360
           +RPH SH+KT  G EL D SSDSDGH+DDKVSS SQS  KSTIYHLLVD LDVSYTIG  
Sbjct: 301 SRPHCSHIKTVTGDELLDESSDSDGHVDDKVSSSSQSYSKSTIYHLLVDGLDVSYTIGYH 360

Query: 361 GDVVVGKVELASPSSRTRCN 381
           GDVVV KVELASPSSRTR N
Sbjct: 361 GDVVVDKVELASPSSRTRSN 380

[Close]

BLAST of HG10008227 vs. ExPASy TrEMBL
Match: A0A6J1BYL5 (uncharacterized protein LOC111006924 OS=Momordica charantia OX=3673 GN=LOC111006924 PE=4 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 5.7e-185
Identity = 329/380 (86.58%), Postives = 357/380 (93.95%), Query Frame = 0


Query: 1   MAYAAESRWSTAAMSVTLAFISASAVISLYLVTKKSRELGTKVRELEASLAASLKKTAAE 60
           MAY AESRWSTAAMS+ LAFISASA++SLYLVT+KSRELG+KVRELEASLAASLKKT AE
Sbjct: 1   MAYGAESRWSTAAMSIILAFISASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTTAE 60

Query: 61  RQGRIRAQQALRKAVAEPKSDSLECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARL 120
           RQGRIRAQQALRKA+A+PKSD++ECTSYPMTPIG I+S+FSTRNGTPRQSL+VPLARARL
Sbjct: 61  RQGRIRAQQALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARL 120

Query: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180
           VFSARIPPASIEGLGEYSHCW+IYVFHLNTDLDKLWK+PSKS+FKAKVRVPRLKG+RVGV
Sbjct: 121 VFSARIPPASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKRVGV 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240
           FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVP+
Sbjct: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPE 240

Query: 241 WLLGDNLLTVASICFSEEFITTLSECWPLMEKKSLYASEDELKNFVKQVLSWDIRSVSQR 300
           WL+GDNLL+VASICFSEEF +TLS+CWPLMEKKS YAS DE KNFVKQVLSWDIRS+SQR
Sbjct: 241 WLMGDNLLSVASICFSEEFTSTLSDCWPLMEKKSHYASADEFKNFVKQVLSWDIRSLSQR 300

Query: 301 NRPHDSHVKTTAGHELSDGSSDSDGHLDDKVSSGSQSSCKSTIYHLLVDELDVSYTIGCD 360
            RPH+SH++TT GHELSDGSSD D HL DK S G+Q S K+ IYHLLVD L+VSYTIGC 
Sbjct: 301 TRPHESHIETTTGHELSDGSSDLDTHLYDKASGGTQISSKNIIYHLLVDGLNVSYTIGCH 360

Query: 361 GDVVVGKVELASPSSRTRCN 381
           GDVVV K ELASPSSRTR N
Sbjct: 361 GDVVVVKAELASPSSRTRSN 380

[Close]

BLAST of HG10008227 vs. ExPASy TrEMBL
Match: A0A6J1JCZ9 (uncharacterized protein LOC111485678 OS=Cucurbita maxima OX=3661 GN=LOC111485678 PE=4 SV=1)

HSP 1 Score: 637.5 bits (1643), Expect = 3.6e-179
Identity = 321/375 (85.60%), Postives = 354/375 (94.40%), Query Frame = 0


Query: 1   MAYAAESRWSTAAMSVTLAFISASAVISLYLVTKKSRELGTKVRELEASLAASLKKTAAE 60
           MAYA  SR STAAMS+TLAF+SASA++SLY VTKKSRELG+KVRELEASLAASLKKT AE
Sbjct: 1   MAYAVVSRSSTAAMSITLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE 60

Query: 61  RQGRIRAQQALRKAVAEPKSDSLECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARL 120
           RQGRIRAQQALRKAV+EPKSDS+ECT YPM PIGTI+SSFSTRNGTPRQSL+VPLARARL
Sbjct: 61  RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL 120

Query: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180
           VFS+RIPPASIEGLGEYSHCWVIYVFHLNT+LDKLWKDPSKSKFKAKV+VPRLKGE+VG+
Sbjct: 121 VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240
           FATRSPHRPCPIG+TIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDS+QDATVPK
Sbjct: 181 FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK 240

Query: 241 WLLGDNLLTVASICFSEEFITTLSECWPLMEKKSLYASEDELKNFVKQVLSWDIRSVSQR 300
           WL G+N+L+VASICFSEEF  +LSECWPL+EKKSLYAS DELK+FVKQVLSWDIRS+SQR
Sbjct: 241 WLTGENVLSVASICFSEEFTPSLSECWPLIEKKSLYASADELKSFVKQVLSWDIRSLSQR 300

Query: 301 NRPHDSHVKTTAGHELSDGSSDSDGHLDDKVSSG-SQSSCKSTIYHLLVDELDVSYTIGC 360
           ++PH+S++ TT GHELSDGSSDSDGHLD+ + SG SQSS KS +YHLLVD L+VSYTIGC
Sbjct: 301 SQPHESYLTTTRGHELSDGSSDSDGHLDETLPSGTSQSSSKSVVYHLLVDGLNVSYTIGC 360

Query: 361 DGDVVVGKVELASPS 375
            GDVVV KVE+ASPS
Sbjct: 361 QGDVVVRKVEVASPS 375

[Close]

BLAST of HG10008227 vs. TAIR 10
Match: AT4G28020.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Uncharacterised protein family UPF0066 (InterPro:IPR001378); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 399.8 bits (1026), Expect = 2.4e-111
Identity = 207/372 (55.65%), Postives = 271/372 (72.85%), Query Frame = 0


Query: 1   MAYAAESRWS-TAAMSVTLAFISASAVISLYLVTKKSRELGTKVRELEASLAASLKKTAA 60
           MA +  S  S TAA++ TLA +S SA  SLY   ++  +L  +++E E SL ++L+K+A+
Sbjct: 1   MATSGRSHGSTTAALAFTLAVVSVSAAFSLYRWRRREEDLENRIKEFEKSLGSTLEKSAS 60

Query: 61  ERQGRIRAQQALRKAVAEPKSDSLECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARAR 120
           ER+GR++AQQALR+A+A+ +S  L  T YPM PIGTI+S FSTRNGTPRQ LLV LARA 
Sbjct: 61  ERKGRVKAQQALREALAQTESHDLHSTIYPMRPIGTIQSCFSTRNGTPRQPLLVSLARAC 120

Query: 121 LVFS-ARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERV 180
           L+F  A +PPAS+EGL EYSHCW++YVFHLNTD++KLW+ PS+SK KAKVRVPRL GER 
Sbjct: 121 LIFDPALVPPASLEGLEEYSHCWILYVFHLNTDIEKLWRKPSQSKLKAKVRVPRLNGERK 180

Query: 181 GVFATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATV 240
           GVFATRSPHRPCPIGLT+AKVE IQ  ++LLSG DLVDGTPVLD+KPYLPY DS+Q A+V
Sbjct: 181 GVFATRSPHRPCPIGLTVAKVEEIQKDKVLLSGVDLVDGTPVLDIKPYLPYSDSIQGASV 240

Query: 241 PKWLLGDNLLTVASICFSEEFITTLSECWPLMEKKSLYASEDELKNFVKQVLSWDIRSVS 300
           P W+  D  L VAS+ FS+ F ++++ CW L+EKKSLY+S DE ++ + QVLSWDIRS+S
Sbjct: 241 PNWVKEDCSLAVASVTFSDTFSSSITSCWKLIEKKSLYSSADEFRSLITQVLSWDIRSMS 300

Query: 301 QRNRPHDSHVKTTAGHELSDGSSDSDGHLDDKVSSGSQSSCKSTIYHLLVDELDVSYTIG 360
           QRN+P D+                    LD+++           +YHL+++ LDVSY I 
Sbjct: 301 QRNKPQDT--------------------LDEEI-----------VYHLVLEGLDVSYMID 341

Query: 361 CDGDVVVGKVEL 371
            + +++V  V L
Sbjct: 361 NESNILVQDVSL 341

The following BLAST results are available for this feature:

BLAST of HG10008227 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038879626.1	1.6e-197	93.95	tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Benincasa hispida]	[more]
TYK09463.1	2.7e-189	90.53	tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Cucumis melo var. makuwa]	[more]
XP_004149885.1	4.6e-189	90.79	uncharacterized protein LOC101219357 [Cucumis sativus] >KGN65285.1 hypothetical ...	[more]
XP_008453657.1	5.1e-188	90.00	PREDICTED: tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Cucumis melo]	[more]
XP_022134721.1	1.2e-184	86.58	uncharacterized protein LOC111006924 [Momordica charantia]	[more]

BLAST of HG10008227 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9BU70	3.7e-32	45.96	tRNA (adenine(37)-N6)-methyltransferase OS=Homo sapiens OX=9606 GN=TRMO PE=1 SV=...	[more]
Q562D6	6.3e-32	45.63	tRNA (adenine(37)-N6)-methyltransferase OS=Mus musculus OX=10090 GN=Trmo PE=2 SV...	[more]
Q4V7E0	1.2e-30	45.22	tRNA (adenine(37)-N6)-methyltransferase OS=Rattus norvegicus OX=10116 GN=Trmo PE...	[more]
Q9RPT0	9.5e-28	43.87	Putative S-adenosylmethionine-dependent methyltransferase RcsF OS=Pseudomonas ae...	[more]
O68638	8.8e-26	41.94	Putative S-adenosylmethionine-dependent methyltransferase RcsF OS=Pseudomonas ae...	[more]

BLAST of HG10008227 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CC84	1.3e-189	90.53	tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo var. makuwa O...	[more]
A0A0A0LTM6	2.2e-189	90.79	TsaA-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G295140 P...	[more]
A0A1S3BW92	2.5e-188	90.00	tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A6J1BYL5	5.7e-185	86.58	uncharacterized protein LOC111006924 OS=Momordica charantia OX=3673 GN=LOC111006...	[more]
A0A6J1JCZ9	3.6e-179	85.60	uncharacterized protein LOC111485678 OS=Cucurbita maxima OX=3661 GN=LOC111485678...	[more]

BLAST of HG10008227 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G28020.1	2.4e-111	55.65	unknown protein; CONTAINS InterPro DOMAIN/s: Uncharacterised protein family UPF0...	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR023370	TrmO-like, N-terminal domain	PFAM	PF01980	TrmO	coord: 105..231 e-value: 6.6E-43 score: 145.7
IPR023370	TrmO-like, N-terminal domain	TIGRFAM	TIGR00104	TIGR00104	coord: 88..236 e-value: 1.9E-28 score: 97.4
IPR023370	TrmO-like, N-terminal domain	PROSITE	PS51668	TSAA_2	coord: 90..235 score: 68.35746
IPR023370	TrmO-like, N-terminal domain	CDD	cd09281	UPF0066	coord: 92..226 e-value: 2.98934E-50 score: 163.046
IPR036414	YaeB, N-terminal domain superfamily	GENE3D	2.40.30.70		coord: 89..242 e-value: 2.3E-53 score: 181.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 303..337
None	No IPR available	PANTHER	PTHR12818:SF0	TRNA (ADENINE(37)-N6)-METHYLTRANSFERASE	coord: 11..370
IPR040372	YaeB-like	PANTHER	PTHR12818	UNCHARACTERIZED	coord: 11..370
IPR036413	YaeB-like superfamily	SUPERFAMILY	118196	YaeB-like	coord: 86..306

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10008227.1	HG10008227.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030488	tRNA methylation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016430	tRNA (adenine-N6-)-methyltransferase activity