HG10008221 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10008221
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionE3 ubiquitin-protein ligase XBAT32
LocationChr10: 20981575 .. 20985668 (-)
RNA-Seq ExpressionHG10008221
SyntenyHG10008221
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAACTTTTAAGTGCTGTGGTAAACTCCTTTGGATGTTCTGCATCTGGGGAACGTTTAGTTTCAGCTGCAAGAGATGGAGATTTTCAAGAAGCACAGGCTTTGTTGGAATATAATCCACGGTTGGCACGTTACTCTACTTTCGGTGTGCGCAATTCCCCACTCCACTACTCTGCAGCTCATGGCCATCACGAGGTATTTCACTCTTTAAATGCACACTGATAATGTTTGTGAAGCCATTCTCACTTCGTTTAAGATCAGAGAATCTTATAGCCTGTTGTCATTGTGCTTGTTCTTACATGTTTGCAGATAGTCTATCTTTTGCTTCAGTCTGGAGTTGACATTAATTTAAGAAACTATAGGGGTCAGGTATTGCCACTTTCTTCAAATTATTTGCCTTTTCATTTCAGGGATCTACATCCATAAGTGTTTTTTCTTCTCTTTTCACCATTTCATTTTCTCCATATTGTAATTATCTGTGCTATTGGACGTTAAGTTGTATCTCCTGCATTTCAAGATTAAATTCATTATGTATTTTGAGTTCATCATTGTTTGCTTTACACTTCTGTACACAGACTGCTTTGATGCAAGCTTGTCAACATGGTCGCTGGGAGGTTGTTTTGATTTTAGTACTTTTTGGAGCCAACGTGAGTAGCTTATCACATTCATTTTAGCTATCACGTTCATAGTGTGAGCAACTTATTAGTCATTCATATATTTGTTTCACATTTTAGGTCCATAAAGCAGATTATTTGAATGGAGGGACTGCACTTCATCTTGCTGCTACAAATGGTCATTCCCGGTGCATCAGGCTTCTTCTTGCTGATTATATTCCAAGCATACCTAATTTTAGGGAAATAATGAGTCAATTAACAGACAATGAAGAATCAATCTCAGAGTTTGATCATAGGTACATATTGAATTCCTCCTCATGAAAATTTTCCAGGTTGAAATGCTTATGATGGTTGATTAACATCATAATTTTGTGGTTTAGGGCACTCTCTCAGATTATTAACCAAACGGCAGATGGAGGCATTACCGCTCTTCACATGGCAGCCCTTAATGGGCAAGTTGAAAGCGTGCAGTTACTTCTGGAATTTGGGGCATCAGTTTCTGAGGTGACTCTAGGCGATGGAACCGTAATTGATCTAATAGGTTTCTCCCTTTGATTTTTTTTGTCTTCTCTTTCACTAAATCAACTTGAGGCCTTCTTTTTCAAGTTAGCCTTTATTTTCCTTCCTAATCCACCACTCAACTTGTTTTCTTTTTTCTTTTAAACTTCTTTTGAGAGTAACAAAACATGAATCTAAAATCTTGACATTATGTTTTGGATTTTCTTCTATTTTCATATAAAGGCTAGAGCTAGCATGCATTGTCATGGGATGTTGAAATGAAGTGGCAGTTAAATGCAATGTTATTTCTTTTGCCTCAGTAAAATATGCTACCTTATCCTTCTATTCTGTGGGCAAGTGAAAAGTACTGATAAAAGGGGTTATTGAAAAGTTGATTTCTTATGATCTCTTGCGATGATATAAATGGCTTGTCTGTTGCAACTTCGGTAGGTGCTGGGAGTACAGCTCTTCATTATGCTGCATGCGGTGGTAATGCAAAATGTTGTCAAGTATGTGTTTCATTTAAAGGAAAAGAATCAGAAATGGGATTAAATGAATGTATTGTGTTTTCTTCTTTGAGAGGCTATTACTTATTGTTTTCTATTTCGTGTAGCTTTTGATTGCGAGGGGGGCCAGTCTGACTGCTGAAAATGAAAATGGGTACGTACTATCCACTTGGCTTATTCTTTGTATGTGAACTTGAATCTTTTTTTTTTTAATCTTTGGACCTGTGCCTAGCAAGTCACCTTCTGAACATAAGAAATTGCTTTTAAGAAATGAGTTTACGTTCTTTTAATTACTTGGGCACTCATTAGGGAACTATTTAAAAAACTGAAAGCGGTAGTGGCTGTGGAGAAATTGATAGGAATGCTGTTAGGATAGAAGTTAAGGATATTATTATGCTAGGAAGGGTATTAGTTTATTTGGTTTAGGCTTGAATGATCTCGAGAGAGGAAGAGTCTATGAACCTCAAATTACTTTAAGAGTTATTGTAGTTTTTCTGTGTATTATTATAATGCCAACTCCAAGTATTTGGGTTCTATCAGAAATTTTGGAGGATTCCCATTAAAATTTTAAAGTGAAACAGTGTCAACAGCCTTGTCAATAAGTATATGCTGGCTTATGTGATTGTCTGGCTAGCTTGAGATGGAAGAATTGGGGTTCAAATTGAAAGCAGTAGCGGCTATGGAGAAATTTTGGAAGACTCCCCTTTTTAGTTCCTTGAAAACACATTACATGAATGCCTCAATTACATAAGAAATTTGTGTGGTTGAACGGTTAATTCATAGGGTTCCCTATTTCCAAGGATATTAATGGGTCACTCATTTCACATCAATAACATTCTGAGAATACTATATAAGTTTTCGATGTTCGTTTAACTACATTAAGCACGGGTATAATCCTTTGATTACACTGATCATTTTGTCATGGAAATATTGTTCCCTACTTTGTATGGAATTGTAGCATTCACTTGATTTAGCATGGTTATCGTATAGCAATCCCTTTATCCATTCTTTTATCAACTTGCATCTACTTCACTTGATTTGTCTTTTTTTCCCACACAGTTGGACACCATTGATGGTCGCTCGCTCATGGAATAAAAATTGGCTTGAGGATATTCTCACCGCAGAGCCAGTAGACCGGCCAAAAGTTATTCCCTCTCGTTACATATCTCTGCCCCTTATGAGTATTGTTCAAATTGCTAGGTAACTGGAATTCGTTATTTCCTTCTATTGCTTATCCTTCCAAAGAAATTTATATACATGGAATCGACTTGCCCTTCATTTATTCATTCTGGAGCCACCAACTTTGAGGACAATATTGTGCGCTTCTGGTTACTACATCATAGAATTTGATCCACATGCTCCTCACTCTGGTCCTCAAGATTAAGTTCATATTGGGTTGACATAGAACAAGATATTGAAGACAAGCTATTAGAACCAGTCCTAAAATATAAGTTGTTTAGAAAATCAAAGAACAAACTAGAAAATTGTTCTCTCTCTACATATAGCTTGCAGATATTGCGTCCAATGAATTTAGAACGTTGTCATGATGAATGAAATTGATTTCGGTATCCCTTTTGGATCCATTTGCAGAGAGTATGGATGGACTATTACGTCTGCCCCCGGATGCCAGGATCCATGCGTTGTCTGTTTAGAGAGGAAGTGCACGGTAGCTGCTGAAGGTATATTATTAACTTAACAATATATATACTTGACTAGAGAATTATGAAATTCTTATTTCCTTAACTTGCATAACCCGATTTATTTATGTTCCATTAAAGTTTTGACAAGTTTGCTGAGTATGAGCAAAAGTTTGAACTTCAATGTTTAAAACATGGGCTATTTTCATATTAGTTAATGAAATTTATTCATAATGGATTTAAAAGTGCAGTGATGGTAAAGATTTGATTGAAAAGAGCTGATATAGATGAAAGTTGAAACAAGCTTTTGAAATAACAAGCTTTTGATATAGATGAAAGTAGTAAACTAAATTCTATACTCAGCCCATCTATTACGTCTATTGGTATGTGGGGTTGTTGAAGTATGATAAGAAGTCCAATCCCCTGCAGGCTGTGGTCATGAATTCTGTACAAGATGTGCCTTATACCTCTGTTCTACAAACTGCGGGACAACCATTTCTCGTGGCCCTCCAGGTTCAGTTTCTTGTCCACTCTGTCGAAATGGCATAGTGTCATTCGTTAAGCTTCCTGGAAAAGCACTTGTTAAAGAGATTGCAAGAACAAGTTTGTCACTTTCAATGTGCCAATGTTCTCACGAGACACCATCAAAACCTACCCCCTTAACCACCCAACTTCAATCGCTCGAGTTGTGTCGTGTCCACGATACTCCTCTGGCAGTTTCCCTTTGCTGTCAGAGACTTCCATCAATCAATCTCTGTCGGAAAACACCGGACATATGCCACTCTTTAGTTCCACGTTGTGCCAACCGGAACTTGCAGAACCACTTGGCCAGATGTTGA

mRNA sequence

ATGAAACTTTTAAGTGCTGTGGTAAACTCCTTTGGATGTTCTGCATCTGGGGAACGTTTAGTTTCAGCTGCAAGAGATGGAGATTTTCAAGAAGCACAGGCTTTGTTGGAATATAATCCACGGTTGGCACGTTACTCTACTTTCGGTGTGCGCAATTCCCCACTCCACTACTCTGCAGCTCATGGCCATCACGAGATAGTCTATCTTTTGCTTCAGTCTGGAGTTGACATTAATTTAAGAAACTATAGGGGTCAGACTGCTTTGATGCAAGCTTGTCAACATGGTCGCTGGGAGGTTGTTTTGATTTTAGTACTTTTTGGAGCCAACGTCCATAAAGCAGATTATTTGAATGGAGGGACTGCACTTCATCTTGCTGCTACAAATGGTCATTCCCGGTGCATCAGGCTTCTTCTTGCTGATTATATTCCAAGCATACCTAATTTTAGGGAAATAATGAGTCAATTAACAGACAATGAAGAATCAATCTCAGAGTTTGATCATAGGGCACTCTCTCAGATTATTAACCAAACGGCAGATGGAGGCATTACCGCTCTTCACATGGCAGCCCTTAATGGGCAAGTTGAAAGCGTGCAGTTACTTCTGGAATTTGGGGCATCAGTTTCTGAGGTGACTCTAGGCGATGGAACCGTAATTGATCTAATAGGTGCTGGGAGTACAGCTCTTCATTATGCTGCATGCGGTGGTAATGCAAAATGTTGTCAACTTTTGATTGCGAGGGGGGCCAGTCTGACTGCTGAAAATGAAAATGGTTGGACACCATTGATGGTCGCTCGCTCATGGAATAAAAATTGGCTTGAGGATATTCTCACCGCAGAGCCAGTAGACCGGCCAAAAGTTATTCCCTCTCGTTACATATCTCTGCCCCTTATGAGTATTGTTCAAATTGCTAGAGAGTATGGATGGACTATTACGTCTGCCCCCGGATGCCAGGATCCATGCGTTGTCTGTTTAGAGAGGAAGTGCACGGTAGCTGCTGAAGGCTGTGGTCATGAATTCTGTACAAGATGTGCCTTATACCTCTGTTCTACAAACTGCGGGACAACCATTTCTCGTGGCCCTCCAGGTTCAGTTTCTTGTCCACTCTGTCGAAATGGCATAGTGTCATTCGTTAAGCTTCCTGGAAAAGCACTTGTTAAAGAGATTGCAAGAACAAGTTTGTCACTTTCAATGTGCCAATGTTCTCACGAGACACCATCAAAACCTACCCCCTTAACCACCCAACTTCAATCGCTCGAGTTGTGTCGTGTCCACGATACTCCTCTGGCAGTTTCCCTTTGCTGTCAGAGACTTCCATCAATCAATCTCTGTCGGAAAACACCGGACATATGCCACTCTTTAGTTCCACGTTGTGCCAACCGGAACTTGCAGAACCACTTGGCCAGATGTTGA

Coding sequence (CDS)

ATGAAACTTTTAAGTGCTGTGGTAAACTCCTTTGGATGTTCTGCATCTGGGGAACGTTTAGTTTCAGCTGCAAGAGATGGAGATTTTCAAGAAGCACAGGCTTTGTTGGAATATAATCCACGGTTGGCACGTTACTCTACTTTCGGTGTGCGCAATTCCCCACTCCACTACTCTGCAGCTCATGGCCATCACGAGATAGTCTATCTTTTGCTTCAGTCTGGAGTTGACATTAATTTAAGAAACTATAGGGGTCAGACTGCTTTGATGCAAGCTTGTCAACATGGTCGCTGGGAGGTTGTTTTGATTTTAGTACTTTTTGGAGCCAACGTCCATAAAGCAGATTATTTGAATGGAGGGACTGCACTTCATCTTGCTGCTACAAATGGTCATTCCCGGTGCATCAGGCTTCTTCTTGCTGATTATATTCCAAGCATACCTAATTTTAGGGAAATAATGAGTCAATTAACAGACAATGAAGAATCAATCTCAGAGTTTGATCATAGGGCACTCTCTCAGATTATTAACCAAACGGCAGATGGAGGCATTACCGCTCTTCACATGGCAGCCCTTAATGGGCAAGTTGAAAGCGTGCAGTTACTTCTGGAATTTGGGGCATCAGTTTCTGAGGTGACTCTAGGCGATGGAACCGTAATTGATCTAATAGGTGCTGGGAGTACAGCTCTTCATTATGCTGCATGCGGTGGTAATGCAAAATGTTGTCAACTTTTGATTGCGAGGGGGGCCAGTCTGACTGCTGAAAATGAAAATGGTTGGACACCATTGATGGTCGCTCGCTCATGGAATAAAAATTGGCTTGAGGATATTCTCACCGCAGAGCCAGTAGACCGGCCAAAAGTTATTCCCTCTCGTTACATATCTCTGCCCCTTATGAGTATTGTTCAAATTGCTAGAGAGTATGGATGGACTATTACGTCTGCCCCCGGATGCCAGGATCCATGCGTTGTCTGTTTAGAGAGGAAGTGCACGGTAGCTGCTGAAGGCTGTGGTCATGAATTCTGTACAAGATGTGCCTTATACCTCTGTTCTACAAACTGCGGGACAACCATTTCTCGTGGCCCTCCAGGTTCAGTTTCTTGTCCACTCTGTCGAAATGGCATAGTGTCATTCGTTAAGCTTCCTGGAAAAGCACTTGTTAAAGAGATTGCAAGAACAAGTTTGTCACTTTCAATGTGCCAATGTTCTCACGAGACACCATCAAAACCTACCCCCTTAACCACCCAACTTCAATCGCTCGAGTTGTGTCGTGTCCACGATACTCCTCTGGCAGTTTCCCTTTGCTGTCAGAGACTTCCATCAATCAATCTCTGTCGGAAAACACCGGACATATGCCACTCTTTAGTTCCACGTTGTGCCAACCGGAACTTGCAGAACCACTTGGCCAGATGTTGA

Protein sequence

MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAAHGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGTALHLAATNGHSRCIRLLLADYIPSIPNFREIMSQLTDNEESISEFDHRALSQIINQTADGGITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCCQLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSIVQIAREYGWTITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRGPPGSVSCPLCRNGIVSFVKLPGKALVKEIARTSLSLSMCQCSHETPSKPTPLTTQLQSLELCRVHDTPLAVSLCCQRLPSINLCRKTPDICHSLVPRCANRNLQNHLARC
Homology
BLAST of HG10008221 vs. NCBI nr
Match: XP_038879942.1 (probable E3 ubiquitin-protein ligase XBOS32 isoform X1 [Benincasa hispida])

HSP 1 Score: 909.1 bits (2348), Expect = 1.6e-260
Identity = 445/469 (94.88%), Postives = 457/469 (97.44%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
           HGHHEIVYLLLQSGVDIN+RNYRGQTALMQACQHG WEVVLILVLFGANVHKADYLNGGT
Sbjct: 61  HGHHEIVYLLLQSGVDINIRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGGT 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSQLTDNEESISEFDHRALSQIINQTADG 180
           ALHLAATNGHSRCIRLLLADYIPS+PNFREIMSQLTDNEESI EFDHRALS+IINQTADG
Sbjct: 121 ALHLAATNGHSRCIRLLLADYIPSMPNFREIMSQLTDNEESILEFDHRALSKIINQTADG 180

Query: 181 GITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCC 240
           GITALHMAALNGQVESVQLLLEFGASVSEVTLGDGT+IDLIGAGSTALHYAACGGNAKCC
Sbjct: 181 GITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTIIDLIGAGSTALHYAACGGNAKCC 240

Query: 241 QLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSIV 300
           QLLIARGASLTA NENGWTPLMVAR+WNK+WLEDILTAEPVD P++IPSRYISLPLMSIV
Sbjct: 241 QLLIARGASLTARNENGWTPLMVARTWNKSWLEDILTAEPVDLPRLIPSRYISLPLMSIV 300

Query: 301 QIAREYGWTITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRGP 360
           QIAREYGWT  SAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALY CSTN GTTISRGP
Sbjct: 301 QIAREYGWTSKSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYFCSTNYGTTISRGP 360

Query: 361 PGSVSCPLCRNGIVSFVKLPGKALVKEIARTSLSLSMCQCSHETPSKPTPLTTQLQSLEL 420
           PGSVSCPLCRNGIVSFVKLPGKA VK+IARTSLSLSMCQCSHETPSKPTPLTTQL+SLEL
Sbjct: 361 PGSVSCPLCRNGIVSFVKLPGKAPVKKIARTSLSLSMCQCSHETPSKPTPLTTQLRSLEL 420

Query: 421 CRVHDTPLAVSLCCQRLPSINLCRKTPDICHSLVPRCANRNLQNHLARC 470
           CRVH TPL VS+CCQRLPSINLC KTPD+CHSLVPRCANRNLQNHLARC
Sbjct: 421 CRVHYTPLGVSVCCQRLPSINLCWKTPDVCHSLVPRCANRNLQNHLARC 469

BLAST of HG10008221 vs. NCBI nr
Match: XP_008443799.1 (PREDICTED: E3 ubiquitin-protein ligase XBAT32 [Cucumis melo] >KAA0035132.1 E3 ubiquitin-protein ligase XBAT32 [Cucumis melo var. makuwa])

HSP 1 Score: 883.2 bits (2281), Expect = 9.5e-253
Identity = 433/470 (92.13%), Postives = 447/470 (95.11%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           MK LSAVV+SFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MKFLSAVVHSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
           HGHHEIVYLLL SGVDINLRNYRGQTALMQACQHG WEVVLILVLFGANVHKADYLNG T
Sbjct: 61  HGHHEIVYLLLHSGVDINLRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGWT 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMS-QLTDNEESISEFDHRALSQIINQTAD 180
           ALHLAATNGHSRCIRLLLADYIPSIPNFREIMS Q TD+EESISEFDHRALSQIINQ AD
Sbjct: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSRQSTDSEESISEFDHRALSQIINQKAD 180

Query: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKC 240
           GGITALHMAALNGQVESVQLLLEFGASVSEVT+GDGTVIDLIGAGSTALHYAACGGNAKC
Sbjct: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC 240

Query: 241 CQLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSI 300
           CQLLIARGASLT ENENGWTPLMV+RSWNK+WLEDILT EPV+RPK+IPSRYISLPLMSI
Sbjct: 241 CQLLIARGASLTTENENGWTPLMVSRSWNKSWLEDILTTEPVERPKLIPSRYISLPLMSI 300

Query: 301 VQIAREYGWTITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRG 360
           VQIAREYGW+  SAPGCQDPCVVCLERKCTVAA GC HEFCTRCALYLCSTNCGTTISRG
Sbjct: 301 VQIAREYGWSSKSAPGCQDPCVVCLERKCTVAAGGCDHEFCTRCALYLCSTNCGTTISRG 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPGKALVKEIARTSLSLSMCQCSHETPSKPTPLTTQLQSLE 420
           PPGSVSCPLCRNGIVSFVKLPGKAL KEIARTSLS  +CQCSHETP KPTPLTTQL+SLE
Sbjct: 361 PPGSVSCPLCRNGIVSFVKLPGKALAKEIARTSLSFPICQCSHETPPKPTPLTTQLRSLE 420

Query: 421 LCRVHDTPLAVSLCCQRLPSINLCRKTPDICHSLVPRCANRNLQNHLARC 470
           LCRVHDTPL +  CC +LPSI+LCRK PDICHSLVPRCAN NLQNHL+RC
Sbjct: 421 LCRVHDTPLGIYFCCHKLPSISLCRKAPDICHSLVPRCANGNLQNHLSRC 470

BLAST of HG10008221 vs. NCBI nr
Match: TYJ95947.1 (E3 ubiquitin-protein ligase XBAT32 [Cucumis melo var. makuwa])

HSP 1 Score: 882.9 bits (2280), Expect = 1.2e-252
Identity = 433/470 (92.13%), Postives = 447/470 (95.11%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           MK LSAVV+SFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MKFLSAVVHSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
           HGHHEIVYLLL SGVDINLRNYRGQTALMQACQHG WEVVLILVLFGANVHKADYLNG T
Sbjct: 61  HGHHEIVYLLLHSGVDINLRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGWT 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMS-QLTDNEESISEFDHRALSQIINQTAD 180
           ALHLAATNGHSRCIRLLLADYIPSIPNFREIMS Q TD+EESISEFDHRALSQIINQ AD
Sbjct: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSRQSTDSEESISEFDHRALSQIINQKAD 180

Query: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKC 240
           GGITALHMAALNGQVESVQLLLEFGASVSEVT+GDGTVIDLIGAGSTALHYAACGGNAKC
Sbjct: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC 240

Query: 241 CQLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSI 300
           CQLLIARGASLT ENENGWTPLMV+RSWNK+WLEDILT EPV+RPK+IPSRYISLPLMSI
Sbjct: 241 CQLLIARGASLTTENENGWTPLMVSRSWNKSWLEDILTTEPVERPKLIPSRYISLPLMSI 300

Query: 301 VQIAREYGWTITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRG 360
           VQIAREYGW+  SAPGCQDPCVVCLERKCTVAA GC HEFCTRCALYLCSTNCGTTISRG
Sbjct: 301 VQIAREYGWSSKSAPGCQDPCVVCLERKCTVAAGGCDHEFCTRCALYLCSTNCGTTISRG 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPGKALVKEIARTSLSLSMCQCSHETPSKPTPLTTQLQSLE 420
           PPGSVSCPLCRNGIVSFVKLPGKAL KEIARTSLS  +CQCSHETP KPTPLTTQL+SLE
Sbjct: 361 PPGSVSCPLCRNGIVSFVKLPGKALAKEIARTSLSFPICQCSHETPLKPTPLTTQLRSLE 420

Query: 421 LCRVHDTPLAVSLCCQRLPSINLCRKTPDICHSLVPRCANRNLQNHLARC 470
           LCRVHDTPL +  CC +LPSI+LCRK PDICHSLVPRCAN NLQNHL+RC
Sbjct: 421 LCRVHDTPLGIYFCCHKLPSISLCRKAPDICHSLVPRCANGNLQNHLSRC 470

BLAST of HG10008221 vs. NCBI nr
Match: XP_004149881.1 (E3 ubiquitin-protein ligase XBAT32 [Cucumis sativus] >KGN65279.1 hypothetical protein Csa_019889 [Cucumis sativus])

HSP 1 Score: 880.6 bits (2274), Expect = 6.2e-252
Identity = 435/470 (92.55%), Postives = 443/470 (94.26%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           MK LSAVV+SFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MKFLSAVVHSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
           HGHHEIVYLLL SGVDINLRNYRGQTALMQACQHG WEVVLILVLFGANVHKADYLNG T
Sbjct: 61  HGHHEIVYLLLHSGVDINLRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGWT 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMS-QLTDNEESISEFDHRALSQIINQTAD 180
           ALHLAATNGHSRCIRLLLADYIPSIPNFREIMS QLTDNEESISEFDHRALSQIINQ AD
Sbjct: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSRQLTDNEESISEFDHRALSQIINQKAD 180

Query: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKC 240
           GGITALHMAALNGQVESVQLLLEFGASVSEVT+GDGTVIDLIGAGSTALHYAACGGNAKC
Sbjct: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC 240

Query: 241 CQLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSI 300
           CQLLIARGASLT ENENGWTPLMV+RSWNKNWLEDILT EPV+RPK+IPSRYISLPLMSI
Sbjct: 241 CQLLIARGASLTTENENGWTPLMVSRSWNKNWLEDILTTEPVERPKLIPSRYISLPLMSI 300

Query: 301 VQIAREYGWTITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRG 360
           VQIAREYGWT  SAPGCQDPCVVCLERKCTVAAEGC HEFCTRCALYLCSTNCGTTISRG
Sbjct: 301 VQIAREYGWTSKSAPGCQDPCVVCLERKCTVAAEGCDHEFCTRCALYLCSTNCGTTISRG 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPGKALVKEIARTSLSLSMCQCSHETPSKPTPLTTQLQSLE 420
           PPGSVSCPLCRNGIVSF KL  KAL KEIART LS  + QCS ETPSKPTPLTTQL+SLE
Sbjct: 361 PPGSVSCPLCRNGIVSFNKLRSKALAKEIARTRLSFPIRQCSQETPSKPTPLTTQLRSLE 420

Query: 421 LCRVHDTPLAVSLCCQRLPSINLCRKTPDICHSLVPRCANRNLQNHLARC 470
           LCRVHDTPL +  CC RLPSINLCRKTPDICHSLVPRCAN N QNHL RC
Sbjct: 421 LCRVHDTPLGIYFCCHRLPSINLCRKTPDICHSLVPRCANGNFQNHLGRC 470

BLAST of HG10008221 vs. NCBI nr
Match: XP_038879943.1 (probable E3 ubiquitin-protein ligase XBOS32 isoform X2 [Benincasa hispida])

HSP 1 Score: 858.2 bits (2216), Expect = 3.3e-245
Identity = 426/469 (90.83%), Postives = 437/469 (93.18%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
           HGHHE                    TALMQACQHG WEVVLILVLFGANVHKADYLNGGT
Sbjct: 61  HGHHE--------------------TALMQACQHGHWEVVLILVLFGANVHKADYLNGGT 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSQLTDNEESISEFDHRALSQIINQTADG 180
           ALHLAATNGHSRCIRLLLADYIPS+PNFREIMSQLTDNEESI EFDHRALS+IINQTADG
Sbjct: 121 ALHLAATNGHSRCIRLLLADYIPSMPNFREIMSQLTDNEESILEFDHRALSKIINQTADG 180

Query: 181 GITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCC 240
           GITALHMAALNGQVESVQLLLEFGASVSEVTLGDGT+IDLIGAGSTALHYAACGGNAKCC
Sbjct: 181 GITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTIIDLIGAGSTALHYAACGGNAKCC 240

Query: 241 QLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSIV 300
           QLLIARGASLTA NENGWTPLMVAR+WNK+WLEDILTAEPVD P++IPSRYISLPLMSIV
Sbjct: 241 QLLIARGASLTARNENGWTPLMVARTWNKSWLEDILTAEPVDLPRLIPSRYISLPLMSIV 300

Query: 301 QIAREYGWTITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRGP 360
           QIAREYGWT  SAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALY CSTN GTTISRGP
Sbjct: 301 QIAREYGWTSKSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYFCSTNYGTTISRGP 360

Query: 361 PGSVSCPLCRNGIVSFVKLPGKALVKEIARTSLSLSMCQCSHETPSKPTPLTTQLQSLEL 420
           PGSVSCPLCRNGIVSFVKLPGKA VK+IARTSLSLSMCQCSHETPSKPTPLTTQL+SLEL
Sbjct: 361 PGSVSCPLCRNGIVSFVKLPGKAPVKKIARTSLSLSMCQCSHETPSKPTPLTTQLRSLEL 420

Query: 421 CRVHDTPLAVSLCCQRLPSINLCRKTPDICHSLVPRCANRNLQNHLARC 470
           CRVH TPL VS+CCQRLPSINLC KTPD+CHSLVPRCANRNLQNHLARC
Sbjct: 421 CRVHYTPLGVSVCCQRLPSINLCWKTPDVCHSLVPRCANRNLQNHLARC 449

BLAST of HG10008221 vs. ExPASy Swiss-Prot
Match: Q6KAE5 (Probable E3 ubiquitin-protein ligase XBOS32 OS=Oryza sativa subsp. japonica OX=39947 GN=XBOS32 PE=2 SV=2)

HSP 1 Score: 563.1 bits (1450), Expect = 2.9e-159
Identity = 289/456 (63.38%), Postives = 343/456 (75.22%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           M  LS V NSFGCSASGERLVSAARDGD QEA+ALLEYNPRLARYSTFG RNSPLHY+AA
Sbjct: 1   MGFLSLVGNSFGCSASGERLVSAARDGDLQEARALLEYNPRLARYSTFGGRNSPLHYAAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
            GHHEIV LLL+SGV+INLRNYRGQTALMQACQ+G WEVV  L+LF ANVH+ DYLNGG+
Sbjct: 61  QGHHEIVSLLLESGVEINLRNYRGQTALMQACQYGHWEVVQTLMLFNANVHRTDYLNGGS 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSQLTDNEESISEFDHRALSQIINQTADG 180
           ALH AA +GH+RC+RL+LADY+PS+PNF   M      E   ++ D   L +++NQ ADG
Sbjct: 121 ALHFAALHGHARCLRLVLADYVPSMPNFWNSMKDSLSEEGPSADLDEDGLFKMVNQKADG 180

Query: 181 GITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCC 240
           G+T LHMAALNG VE VQLLL+ GASV E T+ DGT IDLIGAGST LHYAACGGNA CC
Sbjct: 181 GLTPLHMAALNGHVECVQLLLDLGASVIEATIEDGTTIDLIGAGSTPLHYAACGGNAVCC 240

Query: 241 QLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSIV 300
           QLLIARGASL+A+N +GWTPLMVARSW++N LE+IL+ EP  R + +PS Y+ LPLMSI+
Sbjct: 241 QLLIARGASLSAQNASGWTPLMVARSWHRNSLEEILSKEPESRIRTVPSPYLCLPLMSIM 300

Query: 301 QIAREYGWT-ITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRG 360
            IARE+GW  +  +P C DPC VCLE  C+VAAEGC HEFCTRCALYLCST+  T++S  
Sbjct: 301 SIAREFGWRYLNQSPVCIDPCAVCLEGSCSVAAEGCKHEFCTRCALYLCSTSY-TSVS-- 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPGKALVKEIARTSLSLSMCQCSHETPSKPTP-LTTQLQSL 420
           P G++ CPLCR+ I++F  LPG + ++E+ R SLSLS C       S  TP + + L   
Sbjct: 361 PAGAIPCPLCRHPIIAFTALPGTSPIRELPRNSLSLSFCTTCPAVNSDSTPSIASHLYRT 420

Query: 421 ELCRVHDTPLAV----SLCCQRLPSINLCRKTPDIC 451
           E       P+      SL CQRLP++ L    P  C
Sbjct: 421 EFQCARMPPMGSSSFRSLSCQRLPAMKL---NPSFC 450

BLAST of HG10008221 vs. ExPASy Swiss-Prot
Match: Q6NLQ8 (E3 ubiquitin-protein ligase XBAT32 OS=Arabidopsis thaliana OX=3702 GN=XBAT32 PE=1 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 9.2e-158
Identity = 279/411 (67.88%), Postives = 327/411 (79.56%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           M+ LS V NSFGCSASGERLVSAARDGD QEA+ALL+YNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MRFLSLVGNSFGCSASGERLVSAARDGDLQEAKALLDYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
            GHHEIV LL++SGVDINLRNYRGQTALMQACQHG WEVVLIL+LFGAN+H++DYLNGGT
Sbjct: 61  QGHHEIVSLLVESGVDINLRNYRGQTALMQACQHGHWEVVLILILFGANIHRSDYLNGGT 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSQLTDNEESISEFDHRALSQIINQTADG 180
           ALHLAA NGH RCIR+LL++YIPS+PN     S L + + S++ FD   L ++IN+ ADG
Sbjct: 121 ALHLAALNGHPRCIRILLSEYIPSVPN---CWSLLKNKKTSVAGFDSSVLHEVINRAADG 180

Query: 181 GITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCC 240
           GIT LH+AALNG +E+VQLLL+ GASV++VT+ DGT IDLIGAGSTALHYA+CGGN +CC
Sbjct: 181 GITPLHVAALNGHIETVQLLLDLGASVTQVTVEDGTTIDLIGAGSTALHYASCGGNTQCC 240

Query: 241 QLLIARGASLTAENENGWTPLMVARSWNKNWLEDIL---TAEPVDRPKVIPSRYISLPLM 300
           QLLI++GA L A N NGWTP+MVARSW++NWLE+IL   T +P      +PS ++ LPLM
Sbjct: 241 QLLISKGACLAAVNSNGWTPMMVARSWHRNWLEEILNPTTEQPQLHLPNVPSPFLCLPLM 300

Query: 301 SIVQIAREYGWTITSA-PGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTN-CGTT 360
           SIV IA+E GW        C+DPC VCLERKCTVAA+GC HEFCT CALYL +T+   + 
Sbjct: 301 SIVNIAQECGWRENDCLTPCRDPCAVCLERKCTVAADGCAHEFCTNCALYLSTTSITSSK 360

Query: 361 ISRGPPGSVSCPLCRNGIVSFVKLP---GKALVKEIARTSLSLSMCQCSHE 404
            S   PGSV CPLCRNGIVSF KLP           +RTS+SLS C CS +
Sbjct: 361 TSNVTPGSVPCPLCRNGIVSFTKLPHTTATTRTSTSSRTSISLSFCTCSSD 408

BLAST of HG10008221 vs. ExPASy Swiss-Prot
Match: Q7EZ44 (Probable E3 ubiquitin-protein ligase XBOS35 OS=Oryza sativa subsp. japonica OX=39947 GN=XBOS35 PE=2 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 1.1e-147
Identity = 259/408 (63.48%), Postives = 315/408 (77.21%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           M LL  V +SFGCSA+GERLVSAARDGD QEA ALLE NPRLARYSTFG+RNSPLHYSAA
Sbjct: 1   MGLLGMVGDSFGCSATGERLVSAARDGDIQEAMALLELNPRLARYSTFGIRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
            GHHEIV LL++SGVDINLRN RGQTALMQAC +G W+VV ILVLF AN+HK D  +G T
Sbjct: 61  KGHHEIVSLLIESGVDINLRNCRGQTALMQACLYGHWKVVQILVLFKANIHKKDCFSGAT 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSQLTDNEESISEFDHRALSQIINQTADG 180
           A+H AA  GH+RC+RLL+ADY+PS+P F  +M     +E +   FD  AL ++IN  +DG
Sbjct: 121 AIHFAALKGHTRCLRLLVADYVPSLPEFWSVMHAKCTDETNKEAFDAVALRRLINNKSDG 180

Query: 181 GITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCC 240
           G+T LH+AAL+G  E VQLLL+ GASVSEVT+ DG+ IDLIG+GST LHYAACGG+A CC
Sbjct: 181 GVTPLHLAALHGHAECVQLLLDLGASVSEVTINDGSTIDLIGSGSTPLHYAACGGSAVCC 240

Query: 241 QLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSIV 300
           QLL+A GA++ A+N NG TPLMVARSW+K+ +E ILT       +++PS Y+SLPLMSIV
Sbjct: 241 QLLVAAGANMRAQNTNGLTPLMVARSWHKSSVEGILTKRSEVPVRILPSSYLSLPLMSIV 300

Query: 301 QIAREYGWTITSAPG-CQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRG 360
           +IARE GW  TS    C DPC +CL+ +CTV+AEGCGHEFCT+CALYLC+T   +T  RG
Sbjct: 301 KIARECGWRKTSVSSVCHDPCAICLDTECTVSAEGCGHEFCTKCALYLCATASSSTSIRG 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPGKALVKEIARTSLSLSMCQCSHETPSK 408
            PGS+ CPLCR+ IVSFV+L     +KE+  T+ SL++C     T SK
Sbjct: 361 VPGSIPCPLCRHTIVSFVRLTSTTPIKELPWTNKSLALCAAGASTGSK 408

BLAST of HG10008221 vs. ExPASy Swiss-Prot
Match: Q4FE45 (E3 ubiquitin-protein ligase XBAT33 OS=Arabidopsis thaliana OX=3702 GN=XBAT33 PE=2 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 2.5e-123
Identity = 245/470 (52.13%), Postives = 315/470 (67.02%), Query Frame = 0

Query: 9   NSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAAHGHHEIVY 68
           NSFGCSASGERLVSAARDGDF EA+ LL+ NP LA+YSTFG  NSPLH++AA GH+EIV 
Sbjct: 3   NSFGCSASGERLVSAARDGDFVEAKMLLDCNPCLAKYSTFGGLNSPLHFAAAKGHNEIVG 62

Query: 69  LLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGTALHLAATN 128
           LLL++G D+N RNY GQTALMQAC++G WEVV  L+LF  NV +ADYL G TALH AA N
Sbjct: 63  LLLENGADVNSRNYCGQTALMQACRYGHWEVVQTLLLFRCNVTRADYLAGRTALHFAAVN 122

Query: 129 GHSRCIRLLLADYIPSIPNFREIMSQL--TDNEESISEFDHRALSQIINQTADGGITALH 188
           GH+RCIRL+LAD++PS     + ++ L  T    + ++ +  ALS+ +N+ ADGGITALH
Sbjct: 123 GHARCIRLVLADFLPS-----DKLNSLPETGVVTAKNKSEQSALSKFVNKAADGGITALH 182

Query: 189 MAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCCQLLIAR 248
           MAALNG  + VQLLL+  A+VS VT   GT +D+IGAGST LHYAACGGN KCCQ+L+AR
Sbjct: 183 MAALNGLFDCVQLLLDLEANVSAVTFHYGTSMDMIGAGSTPLHYAACGGNLKCCQILLAR 242

Query: 249 GASLTAENENGWTPLMVARSWNKNWLEDILTA-EPVDRPKVIPSRYISLPLMSIVQIARE 308
           GA     N NGW P+ +AR W+++WLE +L+    V  P    S Y+SLPL+SI+ IARE
Sbjct: 243 GARKMTLNCNGWLPIDIARMWSRHWLEPLLSPNSDVVIPAFPHSNYLSLPLLSILNIARE 302

Query: 309 YGWTITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRGPPGSVS 368
           +G    +     D C VCLER CTVAAEGC H+ C RCALYLCS++   +++ GPPGS+ 
Sbjct: 303 FGLQSATIGDEVDICAVCLERTCTVAAEGCEHQLCVRCALYLCSSSNVPSVTVGPPGSIP 362

Query: 369 CPLCRNGIVSFVKLPGKALVKEIARTSLSLSMC-QCSHET-----PSKPT-PLTTQLQSL 428
           CPLCR+GI +F +LP  +L +E+ +  +SL  C  C   T      S PT P T Q  S 
Sbjct: 363 CPLCRHGITAFKRLP-SSLTREM-KLPMSLGFCAPCMLHTGDTTDQSSPTCPPTEQRSSK 422

Query: 429 ELCRVHDTPLAVSLCCQRLPSIN--LCRKTPDICHSLVPRCANRNLQNHL 467
                  + +   + C   PS+N  +C      C +       R+ + H+
Sbjct: 423 TRAASVSSDIFCPVTCSPFPSVNIPMCTCNEGTCPNFETHGTERHSEEHV 465

BLAST of HG10008221 vs. ExPASy Swiss-Prot
Match: Q337A0 (Probable E3 ubiquitin-protein ligase XBOS33 OS=Oryza sativa subsp. japonica OX=39947 GN=XBOS33 PE=2 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 5.5e-118
Identity = 242/459 (52.72%), Postives = 300/459 (65.36%), Query Frame = 0

Query: 9   NSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAAHGHHEIVY 68
           NS GCSASGERLVSAARDGD  EA+ LLE +P LARYSTFG  NSPLH++AA GH +IV 
Sbjct: 3   NSLGCSASGERLVSAARDGDAVEARMLLELSPALARYSTFGGLNSPLHFAAAKGHLDIVT 62

Query: 69  LLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGTALHLAATN 128
           LLL+ G D+N+RNY GQTALM AC+HG WEVV +L+LF  NV +ADYL+G TALH AA +
Sbjct: 63  LLLEKGADVNVRNYCGQTALMHACRHGHWEVVQMLLLFRCNVTRADYLSGRTALHFAAHD 122

Query: 129 GHSRCIRLLLADYIPSIPNFREIMSQLTDNEESIS------------EFDHRALSQIINQ 188
           G  RC+RLLLAD++PS P   +  S   D  E  +            +F+  A  + IN+
Sbjct: 123 GLVRCVRLLLADFVPSAP-LEDGASSTVDGGECQTNSGSSPCSSLGLKFNESARLRYINK 182

Query: 189 TADGGITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGN 248
            ADGG+TALHMAALNG  + +QLL++ GA+VS VT   GT  +LIGAGST LHYAA GGN
Sbjct: 183 PADGGVTALHMAALNGHFDCMQLLIDLGANVSAVTFPYGTTANLIGAGSTPLHYAAGGGN 242

Query: 249 AKCCQLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDR-PKVIPSRYISLP 308
           A+CCQLL+++GAS    N NGW P+ VAR + + +LE +L+       P   PS Y++LP
Sbjct: 243 AECCQLLLSKGASKLTLNCNGWLPIDVARMFGRRFLEPLLSPNSNSSIPAYQPSNYLALP 302

Query: 309 LMSIVQIAREYGWTITSAP-GCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGT 368
            MSI+ IARE+G   T A     D C VCLER C+VAAEGC HEFC +CALYLCST+   
Sbjct: 303 FMSILNIAREFGLLHTVASVDDSDLCAVCLERSCSVAAEGCCHEFCIKCALYLCSTSNTR 362

Query: 369 TISRGPPGSVSCPLCRNGIVSFVKLPGKALVKEIARTSLSLSMCQ-CSHETPSKPTPLTT 428
               GPPGS+ CPLCRNGI+SF KLP  +   E  ++S +L+ C  C   T S  +P T 
Sbjct: 363 VEFTGPPGSIPCPLCRNGIMSFTKLP--STPTEGLKSSSALTFCNPCMLNTRSVDSPATI 422

Query: 429 QLQSLELCRVH--DTPLAVSLCCQRLPSINL--CRKTPD 449
               +   RV    + L   L C   PS  L  CR + D
Sbjct: 423 SKAEIRRNRVAAVSSELVCPLTCSPFPSSALPTCRCSDD 458

BLAST of HG10008221 vs. ExPASy TrEMBL
Match: A0A5A7SV55 (E3 ubiquitin-protein ligase XBAT32 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold57G002150 PE=4 SV=1)

HSP 1 Score: 883.2 bits (2281), Expect = 4.6e-253
Identity = 433/470 (92.13%), Postives = 447/470 (95.11%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           MK LSAVV+SFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MKFLSAVVHSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
           HGHHEIVYLLL SGVDINLRNYRGQTALMQACQHG WEVVLILVLFGANVHKADYLNG T
Sbjct: 61  HGHHEIVYLLLHSGVDINLRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGWT 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMS-QLTDNEESISEFDHRALSQIINQTAD 180
           ALHLAATNGHSRCIRLLLADYIPSIPNFREIMS Q TD+EESISEFDHRALSQIINQ AD
Sbjct: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSRQSTDSEESISEFDHRALSQIINQKAD 180

Query: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKC 240
           GGITALHMAALNGQVESVQLLLEFGASVSEVT+GDGTVIDLIGAGSTALHYAACGGNAKC
Sbjct: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC 240

Query: 241 CQLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSI 300
           CQLLIARGASLT ENENGWTPLMV+RSWNK+WLEDILT EPV+RPK+IPSRYISLPLMSI
Sbjct: 241 CQLLIARGASLTTENENGWTPLMVSRSWNKSWLEDILTTEPVERPKLIPSRYISLPLMSI 300

Query: 301 VQIAREYGWTITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRG 360
           VQIAREYGW+  SAPGCQDPCVVCLERKCTVAA GC HEFCTRCALYLCSTNCGTTISRG
Sbjct: 301 VQIAREYGWSSKSAPGCQDPCVVCLERKCTVAAGGCDHEFCTRCALYLCSTNCGTTISRG 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPGKALVKEIARTSLSLSMCQCSHETPSKPTPLTTQLQSLE 420
           PPGSVSCPLCRNGIVSFVKLPGKAL KEIARTSLS  +CQCSHETP KPTPLTTQL+SLE
Sbjct: 361 PPGSVSCPLCRNGIVSFVKLPGKALAKEIARTSLSFPICQCSHETPPKPTPLTTQLRSLE 420

Query: 421 LCRVHDTPLAVSLCCQRLPSINLCRKTPDICHSLVPRCANRNLQNHLARC 470
           LCRVHDTPL +  CC +LPSI+LCRK PDICHSLVPRCAN NLQNHL+RC
Sbjct: 421 LCRVHDTPLGIYFCCHKLPSISLCRKAPDICHSLVPRCANGNLQNHLSRC 470

BLAST of HG10008221 vs. ExPASy TrEMBL
Match: A0A1S3B8E1 (E3 ubiquitin-protein ligase XBAT32 OS=Cucumis melo OX=3656 GN=LOC103487302 PE=4 SV=1)

HSP 1 Score: 883.2 bits (2281), Expect = 4.6e-253
Identity = 433/470 (92.13%), Postives = 447/470 (95.11%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           MK LSAVV+SFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MKFLSAVVHSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
           HGHHEIVYLLL SGVDINLRNYRGQTALMQACQHG WEVVLILVLFGANVHKADYLNG T
Sbjct: 61  HGHHEIVYLLLHSGVDINLRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGWT 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMS-QLTDNEESISEFDHRALSQIINQTAD 180
           ALHLAATNGHSRCIRLLLADYIPSIPNFREIMS Q TD+EESISEFDHRALSQIINQ AD
Sbjct: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSRQSTDSEESISEFDHRALSQIINQKAD 180

Query: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKC 240
           GGITALHMAALNGQVESVQLLLEFGASVSEVT+GDGTVIDLIGAGSTALHYAACGGNAKC
Sbjct: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC 240

Query: 241 CQLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSI 300
           CQLLIARGASLT ENENGWTPLMV+RSWNK+WLEDILT EPV+RPK+IPSRYISLPLMSI
Sbjct: 241 CQLLIARGASLTTENENGWTPLMVSRSWNKSWLEDILTTEPVERPKLIPSRYISLPLMSI 300

Query: 301 VQIAREYGWTITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRG 360
           VQIAREYGW+  SAPGCQDPCVVCLERKCTVAA GC HEFCTRCALYLCSTNCGTTISRG
Sbjct: 301 VQIAREYGWSSKSAPGCQDPCVVCLERKCTVAAGGCDHEFCTRCALYLCSTNCGTTISRG 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPGKALVKEIARTSLSLSMCQCSHETPSKPTPLTTQLQSLE 420
           PPGSVSCPLCRNGIVSFVKLPGKAL KEIARTSLS  +CQCSHETP KPTPLTTQL+SLE
Sbjct: 361 PPGSVSCPLCRNGIVSFVKLPGKALAKEIARTSLSFPICQCSHETPPKPTPLTTQLRSLE 420

Query: 421 LCRVHDTPLAVSLCCQRLPSINLCRKTPDICHSLVPRCANRNLQNHLARC 470
           LCRVHDTPL +  CC +LPSI+LCRK PDICHSLVPRCAN NLQNHL+RC
Sbjct: 421 LCRVHDTPLGIYFCCHKLPSISLCRKAPDICHSLVPRCANGNLQNHLSRC 470

BLAST of HG10008221 vs. ExPASy TrEMBL
Match: A0A5D3B9Z5 (E3 ubiquitin-protein ligase XBAT32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold271G00090 PE=4 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 6.0e-253
Identity = 433/470 (92.13%), Postives = 447/470 (95.11%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           MK LSAVV+SFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MKFLSAVVHSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
           HGHHEIVYLLL SGVDINLRNYRGQTALMQACQHG WEVVLILVLFGANVHKADYLNG T
Sbjct: 61  HGHHEIVYLLLHSGVDINLRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGWT 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMS-QLTDNEESISEFDHRALSQIINQTAD 180
           ALHLAATNGHSRCIRLLLADYIPSIPNFREIMS Q TD+EESISEFDHRALSQIINQ AD
Sbjct: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSRQSTDSEESISEFDHRALSQIINQKAD 180

Query: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKC 240
           GGITALHMAALNGQVESVQLLLEFGASVSEVT+GDGTVIDLIGAGSTALHYAACGGNAKC
Sbjct: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC 240

Query: 241 CQLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSI 300
           CQLLIARGASLT ENENGWTPLMV+RSWNK+WLEDILT EPV+RPK+IPSRYISLPLMSI
Sbjct: 241 CQLLIARGASLTTENENGWTPLMVSRSWNKSWLEDILTTEPVERPKLIPSRYISLPLMSI 300

Query: 301 VQIAREYGWTITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRG 360
           VQIAREYGW+  SAPGCQDPCVVCLERKCTVAA GC HEFCTRCALYLCSTNCGTTISRG
Sbjct: 301 VQIAREYGWSSKSAPGCQDPCVVCLERKCTVAAGGCDHEFCTRCALYLCSTNCGTTISRG 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPGKALVKEIARTSLSLSMCQCSHETPSKPTPLTTQLQSLE 420
           PPGSVSCPLCRNGIVSFVKLPGKAL KEIARTSLS  +CQCSHETP KPTPLTTQL+SLE
Sbjct: 361 PPGSVSCPLCRNGIVSFVKLPGKALAKEIARTSLSFPICQCSHETPLKPTPLTTQLRSLE 420

Query: 421 LCRVHDTPLAVSLCCQRLPSINLCRKTPDICHSLVPRCANRNLQNHLARC 470
           LCRVHDTPL +  CC +LPSI+LCRK PDICHSLVPRCAN NLQNHL+RC
Sbjct: 421 LCRVHDTPLGIYFCCHKLPSISLCRKAPDICHSLVPRCANGNLQNHLSRC 470

BLAST of HG10008221 vs. ExPASy TrEMBL
Match: A0A0A0LWB1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G294100 PE=4 SV=1)

HSP 1 Score: 880.6 bits (2274), Expect = 3.0e-252
Identity = 435/470 (92.55%), Postives = 443/470 (94.26%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           MK LSAVV+SFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MKFLSAVVHSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
           HGHHEIVYLLL SGVDINLRNYRGQTALMQACQHG WEVVLILVLFGANVHKADYLNG T
Sbjct: 61  HGHHEIVYLLLHSGVDINLRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGWT 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMS-QLTDNEESISEFDHRALSQIINQTAD 180
           ALHLAATNGHSRCIRLLLADYIPSIPNFREIMS QLTDNEESISEFDHRALSQIINQ AD
Sbjct: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSRQLTDNEESISEFDHRALSQIINQKAD 180

Query: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKC 240
           GGITALHMAALNGQVESVQLLLEFGASVSEVT+GDGTVIDLIGAGSTALHYAACGGNAKC
Sbjct: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC 240

Query: 241 CQLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSI 300
           CQLLIARGASLT ENENGWTPLMV+RSWNKNWLEDILT EPV+RPK+IPSRYISLPLMSI
Sbjct: 241 CQLLIARGASLTTENENGWTPLMVSRSWNKNWLEDILTTEPVERPKLIPSRYISLPLMSI 300

Query: 301 VQIAREYGWTITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRG 360
           VQIAREYGWT  SAPGCQDPCVVCLERKCTVAAEGC HEFCTRCALYLCSTNCGTTISRG
Sbjct: 301 VQIAREYGWTSKSAPGCQDPCVVCLERKCTVAAEGCDHEFCTRCALYLCSTNCGTTISRG 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPGKALVKEIARTSLSLSMCQCSHETPSKPTPLTTQLQSLE 420
           PPGSVSCPLCRNGIVSF KL  KAL KEIART LS  + QCS ETPSKPTPLTTQL+SLE
Sbjct: 361 PPGSVSCPLCRNGIVSFNKLRSKALAKEIARTRLSFPIRQCSQETPSKPTPLTTQLRSLE 420

Query: 421 LCRVHDTPLAVSLCCQRLPSINLCRKTPDICHSLVPRCANRNLQNHLARC 470
           LCRVHDTPL +  CC RLPSINLCRKTPDICHSLVPRCAN N QNHL RC
Sbjct: 421 LCRVHDTPLGIYFCCHRLPSINLCRKTPDICHSLVPRCANGNFQNHLGRC 470

BLAST of HG10008221 vs. ExPASy TrEMBL
Match: A0A6J1E1L3 (probable E3 ubiquitin-protein ligase XBOS32 OS=Cucurbita moschata OX=3662 GN=LOC111430008 PE=4 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 3.9e-244
Identity = 419/468 (89.53%), Postives = 440/468 (94.02%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLE NPRLARYSTFGVRNSPLH+SAA
Sbjct: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLECNPRLARYSTFGVRNSPLHFSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
           HGHHEIV+LLLQSGVDI+LRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT
Sbjct: 61  HGHHEIVHLLLQSGVDIDLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSQLTDNEESISEFDHRALSQIINQTADG 180
           ALHLAATNGHSRCIRLLLADYIPSIPNFRE+MSQ+ DNEE ISEFDH+ALSQ INQ+ADG
Sbjct: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFRELMSQIRDNEEPISEFDHKALSQFINQSADG 180

Query: 181 GITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCC 240
           GITALHMAALN Q ESVQLLLEFGASVSEVT+GDGTVIDLIG GSTALHYAACGGNAKCC
Sbjct: 181 GITALHMAALNAQAESVQLLLEFGASVSEVTIGDGTVIDLIGPGSTALHYAACGGNAKCC 240

Query: 241 QLLIARGASLTAENENGWTPLMVARSWNKNWLEDILTAEPVDRPKVIPSRYISLPLMSIV 300
           +LLIA GASLT EN+NGWTPLMVARSW+K+WLEDIL+ EPV RPK+IPSRY+SLPLMSIV
Sbjct: 241 KLLIAWGASLTTENQNGWTPLMVARSWHKSWLEDILSTEPVGRPKLIPSRYLSLPLMSIV 300

Query: 301 QIAREYGWTITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRGP 360
           QIAREYGW   SAPGCQDPCVVCLERKCTVAAEGC HEFCTRCALYLCSTNCGTTISRGP
Sbjct: 301 QIAREYGWNSKSAPGCQDPCVVCLERKCTVAAEGCDHEFCTRCALYLCSTNCGTTISRGP 360

Query: 361 PGSVSCPLCRNGIVSFVKLPGKALVKEIARTSLSLSMCQCSHETPSKPTPLTTQLQSLEL 420
           PGSVSCPLCRNGIVSFVKLP KALVKE+ARTS  L + Q SHETPSK TPLT+QL+SLEL
Sbjct: 361 PGSVSCPLCRNGIVSFVKLPEKALVKEVARTSFLLPIGQRSHETPSKATPLTSQLRSLEL 420

Query: 421 CRVHDTPLAVSLCCQRLPSINLCRKTPDICHSLVPRCANRNLQNHLAR 469
           C V DT L VS CCQRLPSINLCR+TP+ICHSLVPRC NRNLQNHLAR
Sbjct: 421 CHVDDTSLGVSFCCQRLPSINLCRETPNICHSLVPRCVNRNLQNHLAR 468

BLAST of HG10008221 vs. TAIR 10
Match: AT5G57740.1 (XB3 ortholog 2 in Arabidopsis thaliana )

HSP 1 Score: 558.1 bits (1437), Expect = 6.5e-159
Identity = 279/411 (67.88%), Postives = 327/411 (79.56%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           M+ LS V NSFGCSASGERLVSAARDGD QEA+ALL+YNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MRFLSLVGNSFGCSASGERLVSAARDGDLQEAKALLDYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGT 120
            GHHEIV LL++SGVDINLRNYRGQTALMQACQHG WEVVLIL+LFGAN+H++DYLNGGT
Sbjct: 61  QGHHEIVSLLVESGVDINLRNYRGQTALMQACQHGHWEVVLILILFGANIHRSDYLNGGT 120

Query: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSQLTDNEESISEFDHRALSQIINQTADG 180
           ALHLAA NGH RCIR+LL++YIPS+PN     S L + + S++ FD   L ++IN+ ADG
Sbjct: 121 ALHLAALNGHPRCIRILLSEYIPSVPN---CWSLLKNKKTSVAGFDSSVLHEVINRAADG 180

Query: 181 GITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCC 240
           GIT LH+AALNG +E+VQLLL+ GASV++VT+ DGT IDLIGAGSTALHYA+CGGN +CC
Sbjct: 181 GITPLHVAALNGHIETVQLLLDLGASVTQVTVEDGTTIDLIGAGSTALHYASCGGNTQCC 240

Query: 241 QLLIARGASLTAENENGWTPLMVARSWNKNWLEDIL---TAEPVDRPKVIPSRYISLPLM 300
           QLLI++GA L A N NGWTP+MVARSW++NWLE+IL   T +P      +PS ++ LPLM
Sbjct: 241 QLLISKGACLAAVNSNGWTPMMVARSWHRNWLEEILNPTTEQPQLHLPNVPSPFLCLPLM 300

Query: 301 SIVQIAREYGWTITSA-PGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTN-CGTT 360
           SIV IA+E GW        C+DPC VCLERKCTVAA+GC HEFCT CALYL +T+   + 
Sbjct: 301 SIVNIAQECGWRENDCLTPCRDPCAVCLERKCTVAADGCAHEFCTNCALYLSTTSITSSK 360

Query: 361 ISRGPPGSVSCPLCRNGIVSFVKLP---GKALVKEIARTSLSLSMCQCSHE 404
            S   PGSV CPLCRNGIVSF KLP           +RTS+SLS C CS +
Sbjct: 361 TSNVTPGSVPCPLCRNGIVSFTKLPHTTATTRTSTSSRTSISLSFCTCSSD 408

BLAST of HG10008221 vs. TAIR 10
Match: AT5G07270.1 (XB3 ortholog 3 in Arabidopsis thaliana )

HSP 1 Score: 443.7 bits (1140), Expect = 1.8e-124
Identity = 245/470 (52.13%), Postives = 315/470 (67.02%), Query Frame = 0

Query: 9   NSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAAHGHHEIVY 68
           NSFGCSASGERLVSAARDGDF EA+ LL+ NP LA+YSTFG  NSPLH++AA GH+EIV 
Sbjct: 3   NSFGCSASGERLVSAARDGDFVEAKMLLDCNPCLAKYSTFGGLNSPLHFAAAKGHNEIVG 62

Query: 69  LLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGTALHLAATN 128
           LLL++G D+N RNY GQTALMQAC++G WEVV  L+LF  NV +ADYL G TALH AA N
Sbjct: 63  LLLENGADVNSRNYCGQTALMQACRYGHWEVVQTLLLFRCNVTRADYLAGRTALHFAAVN 122

Query: 129 GHSRCIRLLLADYIPSIPNFREIMSQL--TDNEESISEFDHRALSQIINQTADGGITALH 188
           GH+RCIRL+LAD++PS     + ++ L  T    + ++ +  ALS+ +N+ ADGGITALH
Sbjct: 123 GHARCIRLVLADFLPS-----DKLNSLPETGVVTAKNKSEQSALSKFVNKAADGGITALH 182

Query: 189 MAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCCQLLIAR 248
           MAALNG  + VQLLL+  A+VS VT   GT +D+IGAGST LHYAACGGN KCCQ+L+AR
Sbjct: 183 MAALNGLFDCVQLLLDLEANVSAVTFHYGTSMDMIGAGSTPLHYAACGGNLKCCQILLAR 242

Query: 249 GASLTAENENGWTPLMVARSWNKNWLEDILTA-EPVDRPKVIPSRYISLPLMSIVQIARE 308
           GA     N NGW P+ +AR W+++WLE +L+    V  P    S Y+SLPL+SI+ IARE
Sbjct: 243 GARKMTLNCNGWLPIDIARMWSRHWLEPLLSPNSDVVIPAFPHSNYLSLPLLSILNIARE 302

Query: 309 YGWTITSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYLCSTNCGTTISRGPPGSVS 368
           +G    +     D C VCLER CTVAAEGC H+ C RCALYLCS++   +++ GPPGS+ 
Sbjct: 303 FGLQSATIGDEVDICAVCLERTCTVAAEGCEHQLCVRCALYLCSSSNVPSVTVGPPGSIP 362

Query: 369 CPLCRNGIVSFVKLPGKALVKEIARTSLSLSMC-QCSHET-----PSKPT-PLTTQLQSL 428
           CPLCR+GI +F +LP  +L +E+ +  +SL  C  C   T      S PT P T Q  S 
Sbjct: 363 CPLCRHGITAFKRLP-SSLTREM-KLPMSLGFCAPCMLHTGDTTDQSSPTCPPTEQRSSK 422

Query: 429 ELCRVHDTPLAVSLCCQRLPSIN--LCRKTPDICHSLVPRCANRNLQNHL 467
                  + +   + C   PS+N  +C      C +       R+ + H+
Sbjct: 423 TRAASVSSDIFCPVTCSPFPSVNIPMCTCNEGTCPNFETHGTERHSEEHV 465

BLAST of HG10008221 vs. TAIR 10
Match: AT2G28840.1 (XB3 ortholog 1 in Arabidopsis thaliana )

HSP 1 Score: 134.0 bits (336), Expect = 3.0e-31
Identity = 119/401 (29.68%), Postives = 177/401 (44.14%), Query Frame = 0

Query: 10  SFGCSASGER-LVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAAHGHHEIVY 69
           S  C +  E  + ++ + GD    + ++   P L   +T   R+S LH +AA+G  EI+ 
Sbjct: 4   SMSCGSRPEHGIFASVQCGDIITIRRVMATEPSLLNQTTPYDRHSVLHVAAANGQIEILS 63

Query: 70  LLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGTALHLAATN 129
           LLL+   + +L N   QT LM A  +GR   V  L   GAN+   D +N  T LH AA  
Sbjct: 64  LLLERFTNPDLLNRHKQTPLMLAAMYGRISCVKKLAEVGANILMFDSVNRRTCLHYAAYY 123

Query: 130 GHSRCIRLLLADYIPSIPNFREIMSQLTDNEESISEFDHRALSQIINQTADGGITALHMA 189
           GH+ C++ +L+                    +S     H   ++ +N   D G T LH+A
Sbjct: 124 GHANCVQAILS------------------AAQSSPVAVHWGYARFVNIRDDKGATPLHLA 183

Query: 190 ALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCCQLLIARGA 249
           A   + E V +LL+ G+ V   T   G+       GST LH AA  G+  C + L+A GA
Sbjct: 184 ARQRRPECVNVLLDSGSLVCASTSVYGS------PGSTPLHLAARSGSIDCVRKLLAWGA 243

Query: 250 SLTAENENGWTPLMVARSWNKNWLEDIL---TAEPVDRPKVIPSRYIS--------LPLM 309
                + +G  P +VA          +L   +AEP+  P   P ++IS        L   
Sbjct: 244 DRLQRDASGRIPYVVAMKHKHGACGALLNPSSAEPLVWPS--PLKFISELNDEAKLLLEQ 303

Query: 310 SIVQIAREYGWTITS-------APGCQDP------------CVVCLERKCTVAAEGCGHE 369
           ++++  RE   TI         +P   D             C +C E+ CT+  + CGH+
Sbjct: 304 ALMEANREREKTILKGTAYSLPSPSFSDTDDNMSEVSDTELCCICFEQVCTIEVKDCGHQ 363

Query: 370 FCTRCALYLCSTN--CGTTISRGPPGSVSCPLCRNGIVSFV 378
            C +C L LC  N    TT +  PP    CP CR+ I   V
Sbjct: 364 MCAQCTLALCCHNKPNPTTSTVTPP---VCPFCRSTIACLV 375

BLAST of HG10008221 vs. TAIR 10
Match: AT2G28840.2 (XB3 ortholog 1 in Arabidopsis thaliana )

HSP 1 Score: 115.9 bits (289), Expect = 8.6e-26
Identity = 103/345 (29.86%), Postives = 150/345 (43.48%), Query Frame = 0

Query: 65  EIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGANVHKADYLNGGTALHL 124
           +I+ LLL+   + +L N   QT LM A  +GR   V  L   GAN+   D +N  T LH 
Sbjct: 46  QILSLLLERFTNPDLLNRHKQTPLMLAAMYGRISCVKKLAEVGANILMFDSVNRRTCLHY 105

Query: 125 AATNGHSRCIRLLLADYIPSIPNFREIMSQLTDNEESISEFDHRALSQIINQTADGGITA 184
           AA  GH+ C++ +L+                    +S     H   ++ +N   D G T 
Sbjct: 106 AAYYGHANCVQAILS------------------AAQSSPVAVHWGYARFVNIRDDKGATP 165

Query: 185 LHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDLIGAGSTALHYAACGGNAKCCQLLI 244
           LH+AA   + E V +LL+ G+ V   T   G+       GST LH AA  G+  C + L+
Sbjct: 166 LHLAARQRRPECVNVLLDSGSLVCASTSVYGS------PGSTPLHLAARSGSIDCVRKLL 225

Query: 245 ARGASLTAENENGWTPLMVARSWNKNWLEDIL---TAEPVDRPKVIPSRYIS-------- 304
           A GA     + +G  P +VA          +L   +AEP+  P   P ++IS        
Sbjct: 226 AWGADRLQRDASGRIPYVVAMKHKHGACGALLNPSSAEPLVWPS--PLKFISELNDEAKL 285

Query: 305 LPLMSIVQIAREYGWTITS-------APGCQDP------------CVVCLERKCTVAAEG 364
           L   ++++  RE   TI         +P   D             C +C E+ CT+  + 
Sbjct: 286 LLEQALMEANREREKTILKGTAYSLPSPSFSDTDDNMSEVSDTELCCICFEQVCTIEVKD 345

Query: 365 CGHEFCTRCALYLCSTN--CGTTISRGPPGSVSCPLCRNGIVSFV 378
           CGH+ C +C L LC  N    TT +  PP    CP CR+ I   V
Sbjct: 346 CGHQMCAQCTLALCCHNKPNPTTSTVTPP---VCPFCRSTIACLV 361

BLAST of HG10008221 vs. TAIR 10
Match: AT5G14230.1 (CONTAINS InterPro DOMAIN/s: Ankyrin repeat-containing domain (InterPro:IPR020683), Ankyrin repeat (InterPro:IPR002110); BEST Arabidopsis thaliana protein match is: XB3 ortholog 2 in Arabidopsis thaliana (TAIR:AT5G57740.1); Has 66374 Blast hits to 25358 proteins in 1201 species: Archae - 121; Bacteria - 8133; Metazoa - 29530; Fungi - 5885; Plants - 3349; Viruses - 785; Other Eukaryotes - 18571 (source: NCBI BLink). )

HSP 1 Score: 75.5 bits (184), Expect = 1.3e-13
Identity = 70/249 (28.11%), Postives = 112/249 (44.98%), Query Frame = 0

Query: 49  GVRNSPLHYSAAHGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGRWEVVLILVLFGA 108
           G+   P+H +A  G  EI+  L+  G DIN +N  G TAL+ + +H   E V +L L GA
Sbjct: 339 GIELRPIHIAARDGSVEIIQQLVGFGCDINSKNDVGNTALLISIKHKHPECVKVLALDGA 398

Query: 109 NVHKADYLNGGTALHLAATN----GHSRCI-RLLLADYIPSIPN---FREIMSQLTDNEE 168
           +    +   G +A+ +A +N    G  R I  L+    +P   N   F  ++      + 
Sbjct: 399 DFGLVNKF-GHSAVSIAESNKWSLGLERVILELIRFGVVPHSSNASVFSPLLYGAQAGDA 458

Query: 169 SISEFDHRALSQIINQTADGGITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTVIDL 228
              +   +A    ++   + G +A  +AA+NG VE+ ++L+  GA V        TV+ L
Sbjct: 459 EALKALVKAQDIYLDYQDEEGFSAAMLAAMNGHVEAFRVLVYAGADVKLYNNSGDTVVSL 518

Query: 229 -------------------------IGAGSTALHYAACGGNAKCCQLLIARGASLTAENE 265
                                    +  G  ALH AA  G+ K  +LL  +G SL   + 
Sbjct: 519 SEQNGNRDVIEKVMLEFALEKDSRNMAGGFYALHCAARRGDVKAVKLLSGKGYSLDIPDG 578

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038879942.11.6e-26094.88probable E3 ubiquitin-protein ligase XBOS32 isoform X1 [Benincasa hispida][more]
XP_008443799.19.5e-25392.13PREDICTED: E3 ubiquitin-protein ligase XBAT32 [Cucumis melo] >KAA0035132.1 E3 ub... [more]
TYJ95947.11.2e-25292.13E3 ubiquitin-protein ligase XBAT32 [Cucumis melo var. makuwa][more]
XP_004149881.16.2e-25292.55E3 ubiquitin-protein ligase XBAT32 [Cucumis sativus] >KGN65279.1 hypothetical pr... [more]
XP_038879943.13.3e-24590.83probable E3 ubiquitin-protein ligase XBOS32 isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q6KAE52.9e-15963.38Probable E3 ubiquitin-protein ligase XBOS32 OS=Oryza sativa subsp. japonica OX=3... [more]
Q6NLQ89.2e-15867.88E3 ubiquitin-protein ligase XBAT32 OS=Arabidopsis thaliana OX=3702 GN=XBAT32 PE=... [more]
Q7EZ441.1e-14763.48Probable E3 ubiquitin-protein ligase XBOS35 OS=Oryza sativa subsp. japonica OX=3... [more]
Q4FE452.5e-12352.13E3 ubiquitin-protein ligase XBAT33 OS=Arabidopsis thaliana OX=3702 GN=XBAT33 PE=... [more]
Q337A05.5e-11852.72Probable E3 ubiquitin-protein ligase XBOS33 OS=Oryza sativa subsp. japonica OX=3... [more]
Match NameE-valueIdentityDescription
A0A5A7SV554.6e-25392.13E3 ubiquitin-protein ligase XBAT32 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A1S3B8E14.6e-25392.13E3 ubiquitin-protein ligase XBAT32 OS=Cucumis melo OX=3656 GN=LOC103487302 PE=4 ... [more]
A0A5D3B9Z56.0e-25392.13E3 ubiquitin-protein ligase XBAT32 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A0A0LWB13.0e-25292.55Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G294100 PE=4 SV=1[more]
A0A6J1E1L33.9e-24489.53probable E3 ubiquitin-protein ligase XBOS32 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT5G57740.16.5e-15967.88XB3 ortholog 2 in Arabidopsis thaliana [more]
AT5G07270.11.8e-12452.13XB3 ortholog 3 in Arabidopsis thaliana [more]
AT2G28840.13.0e-3129.68XB3 ortholog 1 in Arabidopsis thaliana [more]
AT2G28840.28.6e-2629.86XB3 ortholog 1 in Arabidopsis thaliana [more]
AT5G14230.11.3e-1328.11CONTAINS InterPro DOMAIN/s: Ankyrin repeat-containing domain (InterPro:IPR020683... [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002110Ankyrin repeatPRINTSPR01415ANKYRINcoord: 118..133
score: 43.69
coord: 196..210
score: 36.31
IPR002110Ankyrin repeatSMARTSM00248ANK_2acoord: 223..252
e-value: 9.7E-5
score: 31.7
coord: 50..79
e-value: 1.7E-5
score: 34.3
coord: 117..147
e-value: 0.21
score: 20.7
coord: 83..112
e-value: 0.027
score: 23.6
coord: 180..209
e-value: 1.6E-4
score: 31.1
IPR002110Ankyrin repeatPFAMPF00023Ankcoord: 224..254
e-value: 1.0E-6
score: 28.9
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 83..115
score: 10.33894
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 50..82
score: 13.11678
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 117..138
score: 9.965
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 180..212
score: 12.52916
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 223..255
score: 11.94154
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 320..369
e-value: 0.0019
score: 27.4
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 320..370
score: 9.951762
IPR020683Ankyrin repeat-containing domainPFAMPF12796Ank_2coord: 88..207
e-value: 1.1E-14
score: 54.7
coord: 20..81
e-value: 2.0E-6
score: 28.3
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 313..389
e-value: 1.9E-6
score: 29.1
IPR036770Ankyrin repeat-containing domain superfamilyGENE3D1.25.40.20coord: 146..283
e-value: 2.6E-27
score: 97.8
IPR036770Ankyrin repeat-containing domain superfamilyGENE3D1.25.40.20coord: 13..145
e-value: 2.5E-32
score: 113.8
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILY48403Ankyrin repeatcoord: 20..277
NoneNo IPR availablePANTHERPTHR24128:SF63E3 UBIQUITIN-PROTEIN LIGASE XBOS32 ISOFORM X1-RELATEDcoord: 1..420
NoneNo IPR availablePANTHERPTHR24128HOMEOBOX PROTEIN WARIAIcoord: 1..420
NoneNo IPR availablePROSITEPS50297ANK_REP_REGIONcoord: 223..255
score: 10.577295
NoneNo IPR availablePROSITEPS50297ANK_REP_REGIONcoord: 50..81
score: 12.009989
NoneNo IPR availablePROSITEPS50297ANK_REP_REGIONcoord: 83..114
score: 9.250726
NoneNo IPR availablePROSITEPS50297ANK_REP_REGIONcoord: 180..208
score: 12.089582
NoneNo IPR availablePROSITEPS50297ANK_REP_REGIONcoord: 117..138
score: 9.940542
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 319..376
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 335..344

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10008221.1HG10008221.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006464 cellular protein modification process
biological_process GO:0016310 phosphorylation
molecular_function GO:0140096 catalytic activity, acting on a protein
molecular_function GO:0016301 kinase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0005515 protein binding