HG10008099 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10008099
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationChr10: 19716683 .. 19718020 (-)
RNA-Seq ExpressionHG10008099
SyntenyHG10008099
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGGAACTAACCCTTCCGGCACTGTCCAAGATGTAGCTTCTAAAGGGGAAGTCCCGGAAAGATACATCCATAAAGAAAGCGATCGAGGAGCTCTAAATGCTCCTTTAATGGCAGCTCCTGTAATCGATATCGCTCTCCTCTCCTCTTCATCCAAATCCGGACCGGAACTGGAGAAACTCCGACATGGACTTCAGTCATGGGGCTGCTTTCAGGTTCAATTTCTGTTTATTTTTCTCTCTAAAACCCTAATTGGGGGGGTAGGAATTTCTATAAACCCTAATTTCTGTGGACTCTGTATCTCGATCCCATTAAATATCAAATTGAAGAAATTAGTAAGTGTTTGTGGTGTTTTTATAGGCTATAAATCATGGAATGTCAACTGAATTTCTTTATGAAGTTCGTCAATTAGCGAAACAGTTCTTTGGTCTTCCAATGAAAGAGAAATTGAAATACTCAAGAGAAGAAGATGAAATGGAAGGATATGGCAATGACATGGTTCTTTCAAACGAACAAATTCTTGATTGGACTGATCGTTTATACCTTACTGTATATCCACAACAAAGCCGTCGTTCCAAGTATTGGCCAACCAATCCTGAAAGATTTAGGTACTTTTCTTTCTCAAATCTTGTTTTCTATATCATTTTTTTTTTATGAATAATGTAAAAGAATCTGCTAGATTGATGAACATTGTAGGGAAGTTGTTGATGAGTACACTGATAATGTGAGGCTGATAAGTGAGAAAATTCTTAAGGCCATGGCAAGGTCATTGGATTTAGATGAGAGTAGCTTTCTTAACCAGTACGGTGAGCGAGTTAAGCTTGACGCACGGTTCAACTTCTACCCTCGATGTCGAAATCCCGATCTTGTTCTCGGTGTTAAGCCGCACGCTGATGGATCGGCAATCACCATTTTGTTGCAGGATAAGGAAGTAGAAGGTCTGCAGTTCATGAAAGGTAACGAGTGGTTCAATGCTGCAATCATTCCTGACGCTCTTCTTGTCAATGTCGGGGATCAAGCAGAGGTAAACACTCTGTACAAAACGAATAATTAATTCTAAAATCACTGTCAAACATGTTGGTAAATGTATATTGTGACAGATCACAAGTAATGGGATATTCAAGAGTCCAGTTCATAGAGTATTGACAAACTCAGAGAGGGAAAGGATATCTTTGGCAGTGTTTTATCTTCCAGATTCAGAGAAAGAAATTGAACCATTAGAGAAACTGATTAACGAAACTCAGCCTAGATTGTACAAGACTGTCAAGAACTTTGTTGGGCTTTACTTTGAGTACTATCAACAGGGCCAGAGACCAATGGAGGCTGCAAGAATCTAA

mRNA sequence

ATGGCCGGAACTAACCCTTCCGGCACTGTCCAAGATGTAGCTTCTAAAGGGGAAGTCCCGGAAAGATACATCCATAAAGAAAGCGATCGAGGAGCTCTAAATGCTCCTTTAATGGCAGCTCCTGTAATCGATATCGCTCTCCTCTCCTCTTCATCCAAATCCGGACCGGAACTGGAGAAACTCCGACATGGACTTCAGTCATGGGGCTGCTTTCAGGCTATAAATCATGGAATGTCAACTGAATTTCTTTATGAAGTTCGTCAATTAGCGAAACAGTTCTTTGGTCTTCCAATGAAAGAGAAATTGAAATACTCAAGAGAAGAAGATGAAATGGAAGGATATGGCAATGACATGGTTCTTTCAAACGAACAAATTCTTGATTGGACTGATCGTTTATACCTTACTGTATATCCACAACAAAGCCGTCGTTCCAAGTATTGGCCAACCAATCCTGAAAGATTTAGGGAAGTTGTTGATGAGTACACTGATAATGTGAGGCTGATAAGTGAGAAAATTCTTAAGGCCATGGCAAGGTCATTGGATTTAGATGAGAGTAGCTTTCTTAACCAGTACGGTGAGCGAGTTAAGCTTGACGCACGGTTCAACTTCTACCCTCGATGTCGAAATCCCGATCTTGTTCTCGGTGTTAAGCCGCACGCTGATGGATCGGCAATCACCATTTTGTTGCAGGATAAGGAAGTAGAAGGTCTGCAGTTCATGAAAGGTAACGAGTGGTTCAATGCTGCAATCATTCCTGACGCTCTTCTTGTCAATGTCGGGGATCAAGCAGAGATCACAAGTAATGGGATATTCAAGAGTCCAGTTCATAGAGTATTGACAAACTCAGAGAGGGAAAGGATATCTTTGGCAGTGTTTTATCTTCCAGATTCAGAGAAAGAAATTGAACCATTAGAGAAACTGATTAACGAAACTCAGCCTAGATTGTACAAGACTGTCAAGAACTTTGTTGGGCTTTACTTTGAGTACTATCAACAGGGCCAGAGACCAATGGAGGCTGCAAGAATCTAA

Coding sequence (CDS)

ATGGCCGGAACTAACCCTTCCGGCACTGTCCAAGATGTAGCTTCTAAAGGGGAAGTCCCGGAAAGATACATCCATAAAGAAAGCGATCGAGGAGCTCTAAATGCTCCTTTAATGGCAGCTCCTGTAATCGATATCGCTCTCCTCTCCTCTTCATCCAAATCCGGACCGGAACTGGAGAAACTCCGACATGGACTTCAGTCATGGGGCTGCTTTCAGGCTATAAATCATGGAATGTCAACTGAATTTCTTTATGAAGTTCGTCAATTAGCGAAACAGTTCTTTGGTCTTCCAATGAAAGAGAAATTGAAATACTCAAGAGAAGAAGATGAAATGGAAGGATATGGCAATGACATGGTTCTTTCAAACGAACAAATTCTTGATTGGACTGATCGTTTATACCTTACTGTATATCCACAACAAAGCCGTCGTTCCAAGTATTGGCCAACCAATCCTGAAAGATTTAGGGAAGTTGTTGATGAGTACACTGATAATGTGAGGCTGATAAGTGAGAAAATTCTTAAGGCCATGGCAAGGTCATTGGATTTAGATGAGAGTAGCTTTCTTAACCAGTACGGTGAGCGAGTTAAGCTTGACGCACGGTTCAACTTCTACCCTCGATGTCGAAATCCCGATCTTGTTCTCGGTGTTAAGCCGCACGCTGATGGATCGGCAATCACCATTTTGTTGCAGGATAAGGAAGTAGAAGGTCTGCAGTTCATGAAAGGTAACGAGTGGTTCAATGCTGCAATCATTCCTGACGCTCTTCTTGTCAATGTCGGGGATCAAGCAGAGATCACAAGTAATGGGATATTCAAGAGTCCAGTTCATAGAGTATTGACAAACTCAGAGAGGGAAAGGATATCTTTGGCAGTGTTTTATCTTCCAGATTCAGAGAAAGAAATTGAACCATTAGAGAAACTGATTAACGAAACTCAGCCTAGATTGTACAAGACTGTCAAGAACTTTGTTGGGCTTTACTTTGAGTACTATCAACAGGGCCAGAGACCAATGGAGGCTGCAAGAATCTAA

Protein sequence

MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIALLSSSSKSGPELEKLRHGLQSWGCFQAINHGMSTEFLYEVRQLAKQFFGLPMKEKLKYSREEDEMEGYGNDMVLSNEQILDWTDRLYLTVYPQQSRRSKYWPTNPERFREVVDEYTDNVRLISEKILKAMARSLDLDESSFLNQYGERVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFMKGNEWFNAAIIPDALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKEIEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI
Homology
BLAST of HG10008099 vs. NCBI nr
Match: XP_038878494.1 (probable 2-oxoglutarate-dependent dioxygenase ANS [Benincasa hispida])

HSP 1 Score: 645.6 bits (1664), Expect = 2.4e-181
Identity = 322/342 (94.15%), Postives = 329/342 (96.20%), Query Frame = 0

Query: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIALLSSSSKSGPELEK 60
           MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDI L S    SGPELEK
Sbjct: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIGLFS----SGPELEK 60

Query: 61  LRHGLQSWGCFQAINHGMSTEFLYEVRQLAKQFFGLPMKEKLKYSREEDEMEGYGNDMVL 120
           LRHGLQSWGCFQAINHGMSTEFL EVRQLAKQFFGLPM+EKLKYSR EDEMEGYGNDMVL
Sbjct: 61  LRHGLQSWGCFQAINHGMSTEFLDEVRQLAKQFFGLPMEEKLKYSR-EDEMEGYGNDMVL 120

Query: 121 SNEQILDWTDRLYLTVYPQQSRRSKYWPTNPERFREVVDEYTDNVRLISEKILKAMARSL 180
           S++QILDWTDRLYLTV PQQSRR KYWPTNPERFREVVDEYT NV+LISEKILKAMARSL
Sbjct: 121 SDQQILDWTDRLYLTVCPQQSRRFKYWPTNPERFREVVDEYTANVKLISEKILKAMARSL 180

Query: 181 DLDESSFLNQYGERVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 240
           DLDESSFLNQYGER++LDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM
Sbjct: 181 DLDESSFLNQYGERIQLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 240

Query: 241 KGNEWFNAAIIPDALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 300
           KGNEWFNA IIP ALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDS+KE
Sbjct: 241 KGNEWFNAPIIPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSDKE 300

Query: 301 IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 343
           IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI
Sbjct: 301 IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 337

BLAST of HG10008099 vs. NCBI nr
Match: KAA0050082.1 (protein SRG1-like [Cucumis melo var. makuwa])

HSP 1 Score: 641.3 bits (1653), Expect = 4.6e-180
Identity = 313/342 (91.52%), Postives = 328/342 (95.91%), Query Frame = 0

Query: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIALLSSSSKSGPELEK 60
           MA TNPSGTVQDVASKGEVPERYIHKESDRGA NAPLMAAPVIDIALLSSSSKSGPELEK
Sbjct: 20  MASTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEK 79

Query: 61  LRHGLQSWGCFQAINHGMSTEFLYEVRQLAKQFFGLPMKEKLKYSREEDEMEGYGNDMVL 120
           LRHGLQSWGCFQAINHGM++E+L EVRQL KQFFGL M+EKLKY +EE EMEGYGNDM+L
Sbjct: 80  LRHGLQSWGCFQAINHGMTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMIL 139

Query: 121 SNEQILDWTDRLYLTVYPQQSRRSKYWPTNPERFREVVDEYTDNVRLISEKILKAMARSL 180
           SN+QILDWTDRLYLTVYP QSRR KYWPTNP+RFREVV EYT NV+LISEKILKAMARSL
Sbjct: 140 SNQQILDWTDRLYLTVYPHQSRRFKYWPTNPQRFREVVGEYTTNVKLISEKILKAMARSL 199

Query: 181 DLDESSFLNQYGERVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 240
           DLDESSFLNQYGE+VKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM
Sbjct: 200 DLDESSFLNQYGEQVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 259

Query: 241 KGNEWFNAAIIPDALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 300
           K NEW+NA I+PDALLVNVGDQ EITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE
Sbjct: 260 KDNEWYNAPIVPDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 319

Query: 301 IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 343
           IEPLE+LINE+QPRLYK+VKNFVGLYFEYYQQGQRPMEAARI
Sbjct: 320 IEPLEELINESQPRLYKSVKNFVGLYFEYYQQGQRPMEAARI 361

BLAST of HG10008099 vs. NCBI nr
Match: TYK10346.1 (protein SRG1-like [Cucumis melo var. makuwa])

HSP 1 Score: 634.8 bits (1636), Expect = 4.3e-178
Identity = 310/342 (90.64%), Postives = 326/342 (95.32%), Query Frame = 0

Query: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIALLSSSSKSGPELEK 60
           MA TNPSGTVQDVASKGEVPERYIHKESDRGA NAPLMAAPVIDIALLSSSSKSGPELEK
Sbjct: 20  MASTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEK 79

Query: 61  LRHGLQSWGCFQAINHGMSTEFLYEVRQLAKQFFGLPMKEKLKYSREEDEMEGYGNDMVL 120
           LRHGLQSWGCFQAINHGM++E+L EVRQL KQFFGL M+EKLKY +EE EMEGYGNDM+L
Sbjct: 80  LRHGLQSWGCFQAINHGMTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMIL 139

Query: 121 SNEQILDWTDRLYLTVYPQQSRRSKYWPTNPERFREVVDEYTDNVRLISEKILKAMARSL 180
           SN+QILDWTDRLYLTVYP QSRR KYWP NP+RFREVV EYT NV+LISEKILKAMARSL
Sbjct: 140 SNQQILDWTDRLYLTVYPHQSRRFKYWPINPQRFREVVGEYTTNVKLISEKILKAMARSL 199

Query: 181 DLDESSFLNQYGERVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 240
           DLDESSFLNQYGE+VKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM
Sbjct: 200 DLDESSFLNQYGEQVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 259

Query: 241 KGNEWFNAAIIPDALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 300
           K NEW+NA I+ DALLVNVGDQ EITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE
Sbjct: 260 KDNEWYNAPIVTDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 319

Query: 301 IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 343
           IEPLE+LINE+QPRLYK+VKNFVGLYFEYYQQG+RPMEAARI
Sbjct: 320 IEPLEELINESQPRLYKSVKNFVGLYFEYYQQGERPMEAARI 361

BLAST of HG10008099 vs. NCBI nr
Match: XP_008465535.1 (PREDICTED: protein SRG1-like [Cucumis melo] >XP_016903407.1 PREDICTED: protein SRG1-like [Cucumis melo])

HSP 1 Score: 633.6 bits (1633), Expect = 9.6e-178
Identity = 309/342 (90.35%), Postives = 327/342 (95.61%), Query Frame = 0

Query: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIALLSSSSKSGPELEK 60
           MA TNPSGTVQDVASKGEVPERYIHKESDRGA NAPLMAAPVIDIALLSSSSKSGPELEK
Sbjct: 20  MASTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEK 79

Query: 61  LRHGLQSWGCFQAINHGMSTEFLYEVRQLAKQFFGLPMKEKLKYSREEDEMEGYGNDMVL 120
           LRHGLQSWGCFQAINHGM++E+L EVRQL KQFFGL M+EKLKY +EE EMEGYGNDM+L
Sbjct: 80  LRHGLQSWGCFQAINHGMTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMIL 139

Query: 121 SNEQILDWTDRLYLTVYPQQSRRSKYWPTNPERFREVVDEYTDNVRLISEKILKAMARSL 180
           SN+QILDWTDRLYLTVYP QSRR KYWPTNP+RFREVV EYT NV+LISEKILKAMARSL
Sbjct: 140 SNQQILDWTDRLYLTVYPHQSRRFKYWPTNPQRFREVVGEYTTNVKLISEKILKAMARSL 199

Query: 181 DLDESSFLNQYGERVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 240
           DLDESSFL+QYGE+VKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDK+VEGLQFM
Sbjct: 200 DLDESSFLSQYGEQVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKKVEGLQFM 259

Query: 241 KGNEWFNAAIIPDALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 300
           K NEW+NA I+ DALLVNVGDQ EITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE
Sbjct: 260 KDNEWYNAPIVTDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 319

Query: 301 IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 343
           IEPLE+LINE+QPRLYK+VKNFVGLYFEYYQQG+RPMEAARI
Sbjct: 320 IEPLEELINESQPRLYKSVKNFVGLYFEYYQQGERPMEAARI 361

BLAST of HG10008099 vs. NCBI nr
Match: XP_011655688.2 (probable 2-oxoglutarate-dependent dioxygenase ANS [Cucumis sativus] >KAE8653027.1 hypothetical protein Csa_019640 [Cucumis sativus])

HSP 1 Score: 631.3 bits (1627), Expect = 4.8e-177
Identity = 308/342 (90.06%), Postives = 326/342 (95.32%), Query Frame = 0

Query: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIALLSSSSKSGPELEK 60
           MAGTNPSGTVQDVASKGEVPERYIHKESDRGA NAPLMAAPVIDIALLSSSSKSGPELEK
Sbjct: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEK 60

Query: 61  LRHGLQSWGCFQAINHGMSTEFLYEVRQLAKQFFGLPMKEKLKYSREEDEMEGYGNDMVL 120
           LRHGLQSWGCFQAINHGM++E+L EVR+L KQFFGL M+EKLKY +EE EMEGYGNDM+L
Sbjct: 61  LRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGLSMEEKLKYLKEELEMEGYGNDMIL 120

Query: 121 SNEQILDWTDRLYLTVYPQQSRRSKYWPTNPERFREVVDEYTDNVRLISEKILKAMARSL 180
           SN+QILDWTDRLYLTVYP QSRR KYWPTN +RFREV+DEYT NV+LISEKI KAMARSL
Sbjct: 121 SNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFREVIDEYTTNVKLISEKIFKAMARSL 180

Query: 181 DLDESSFLNQYGERVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 240
           DLDESSFL QYGE++KLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM
Sbjct: 181 DLDESSFLKQYGEQIKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 240

Query: 241 KGNEWFNAAIIPDALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 300
           K NEWFNA I+PDALLVNVGDQ EITSNGIFKSPVHRVLTNSERERISLAVFYLPD+EKE
Sbjct: 241 KDNEWFNAPIVPDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDAEKE 300

Query: 301 IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 343
           IEPLE+LI+ETQPRLYK+VKNFVGL FEYYQQGQRPMEAARI
Sbjct: 301 IEPLEELISETQPRLYKSVKNFVGLSFEYYQQGQRPMEAARI 342

BLAST of HG10008099 vs. ExPASy Swiss-Prot
Match: O80449 (Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 1.5e-61
Identity = 121/321 (37.69%), Postives = 186/321 (57.94%), Query Frame = 0

Query: 6   PSGTVQDVASKG--EVPERYIHKESDRGALNAPLMAAPVIDIALLSSSSKSG-PE-LEKL 65
           P  +VQ ++  G   VP RY+     R   N     A  I+I +L  +   G PE L  +
Sbjct: 8   PIVSVQSLSQTGVPTVPNRYVKPAHQRPVFNTTQSDAG-IEIPVLDMNDVWGKPEGLRLV 67

Query: 66  RHGLQSWGCFQAINHGMSTEFLYEVRQLAKQFFGLPMKEKLKYSREEDEMEGYGNDMVLS 125
           R   + WG FQ +NHG++   +  VR   ++FF LP++EK KY+   D  EGYG+ + + 
Sbjct: 68  RSACEEWGFFQMVNHGVTHSLMERVRGAWREFFELPLEEKRKYANSPDTYEGYGSRLGVV 127

Query: 126 NEQILDWTDRLYLTVYPQQSRRSKYWPTNPERFREVVDEYTDNVRLISEKILKAMARSLD 185
            +  LDW+D  +L   P   R    WP+ P + RE++++Y + VR + E++ + ++ SL 
Sbjct: 128 KDAKLDWSDYFFLNYLPSSIRNPSKWPSQPPKIRELIEKYGEEVRKLCERLTETLSESLG 187

Query: 186 LDESSFLNQY--GERVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQF 245
           L  +  +     G++V    R NFYP+C  P L LG+  H+D   ITILL D++V GLQ 
Sbjct: 188 LKPNKLMQALGGGDKVGASLRTNFYPKCPQPQLTLGLSSHSDPGGITILLPDEKVAGLQV 247

Query: 246 MKGNEWFNAAIIPDALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEK 305
            +G+ W     +P+AL+VN+GDQ +I SNGI+KS  H+V+ NS  ER+SLA FY P S+ 
Sbjct: 248 RRGDGWVTIKSVPNALIVNIGDQLQILSNGIYKSVEHQVIVNSGMERVSLAFFYNPRSDI 307

Query: 306 EIEPLEKLINETQPRLYKTVK 321
            + P+E+L+   +P LYK ++
Sbjct: 308 PVGPIEELVTANRPALYKPIR 327

BLAST of HG10008099 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 2.1e-58
Identity = 114/330 (34.55%), Postives = 190/330 (57.58%), Query Frame = 0

Query: 19  VPERYIHKESDRGALNAPL---MAAPVIDIALLSSSSKSGPELEKLRHGLQSWGCFQAIN 78
           VP RY+  + D+  ++      +  P+ID+  L SS+    E+EKL    + WG FQ +N
Sbjct: 29  VPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDSEVEKLDFACKEWGFFQLVN 88

Query: 79  HGMSTEFLYEVRQLAKQFFGLPMKEKLKYSREEDEMEGYGNDMVLSNEQILDWTDRLYLT 138
           HG+ + FL +V+   + FF LPM+EK K+ +  DE+EG+G   V+S +Q LDW D  + T
Sbjct: 89  HGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQAFVVSEDQKLDWADLFFHT 148

Query: 139 VYPQQSRRSKYWPTNPERFREVVDEYTDNVRLISEKILKAMARSLDLDESSFLNQYGERV 198
           V P + R+   +P  P  FR+ ++ Y+  V+ +++ ++  MAR+L++        + +  
Sbjct: 149 VQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKMARALEIKPEELEKLFDDVD 208

Query: 199 KLDA-RFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFMKGNEWFNAAIIPDA 258
            + + R N+YP C  PD V+G+ PH+D   +T+L+Q  +VEGLQ  K  +W     +P+A
Sbjct: 209 SVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVNDVEGLQIKKDGKWVPVKPLPNA 268

Query: 259 LLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKEIEPLEKLINETQPR 318
            +VN+GD  EI +NG ++S  HR + NSE+ER+S+A F+     KE+ P + L+   +  
Sbjct: 269 FIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHNVGMYKEVGPAKSLVERQKVA 328

Query: 319 LYK--TVKNFVGLYFEYYQQGQRPMEAARI 343
            +K  T+K +    F     G+  ++A RI
Sbjct: 329 RFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

BLAST of HG10008099 vs. ExPASy Swiss-Prot
Match: Q94LP4 (2-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD11 PE=1 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 3.3e-56
Identity = 115/329 (34.95%), Postives = 181/329 (55.02%), Query Frame = 0

Query: 19  VPERYIHKE-SDRGALN--APLMAAPVIDIALLSSSSKSGPELEKLRHGLQSWGCFQAIN 78
           +PERYI  E S    +N     MA P+ID+  L     S  E  KLR   Q WG F  IN
Sbjct: 29  IPERYIRPEASSEEVINNYQGDMAIPIIDLKKLLCPQSSEEECVKLRSACQYWGFFLLIN 88

Query: 79  HGMSTEFLYEVRQLAKQFFGLPMKEKLKYSREEDEMEGYGNDMVLSNEQILDWTDRLYLT 138
           HG+  E +  +++    FF  P+  K +Y++  + +EGYG   V S +Q LDW D LYL 
Sbjct: 89  HGVPDEVIANLKRDIVDFFSQPLDTKKEYTQLPNSLEGYGQSFVFSEDQKLDWADMLYLH 148

Query: 139 VYPQQSRRSKYWPTNPERFREVVDEYTDNVRLISEKILKAMARSLDLDESSFLNQYGERV 198
           V+P  SR  ++WPT+P  FR+ +D Y+   + ++  + + MA+++     S L+ + E+ 
Sbjct: 149 VHPSDSRDLRFWPTSPASFRQSIDAYSSETKSLALCLFEFMAKAVGAKPESLLDLFEEQP 208

Query: 199 KLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFMKGNEWFNAAIIPDAL 258
           +   R  +YP CR  D V+G+ PH+D   +T+LL+   V+GLQ  K  +WF+      AL
Sbjct: 209 R-GLRMAYYPPCRQADKVMGLSPHSDAGGLTLLLEINNVQGLQIKKDGKWFSIDAPNGAL 268

Query: 259 LVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKEIEPLEKLINETQPRL 318
           + N+GD  EI SNG F+S  HR + N  +ERIS A+F+ P     I PL + + + + + 
Sbjct: 269 IANIGDTLEILSNGKFRSVEHRAVINPNKERISAALFHYPSENMVISPLPEFVKDGKVK- 328

Query: 319 YKTVK--NFVGLYFEYYQQGQRPMEAARI 343
           Y+++   +F+   F     G+  +E  ++
Sbjct: 329 YRSISYLDFMKQIFTQQLDGKNRVEVLKL 355

BLAST of HG10008099 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.6e-55
Identity = 114/319 (35.74%), Postives = 174/319 (54.55%), Query Frame = 0

Query: 6   PSGTVQDVASK--GEVPERYIHKESDRGALNAPLMAA---PVIDIALLSSSSKSGPE--L 65
           P   VQ +A      +P+RYI   S R         A   P+ID+  L S      +  +
Sbjct: 23  PIVRVQSLAESNLSSLPDRYIKPASLRPTTTEDAPTATNIPIIDLEGLFSEEGLSDDVIM 82

Query: 66  EKLRHGLQSWGCFQAINHGMSTEFLYEVRQLAKQFFGLPMKEKLKYSREEDEMEGYGNDM 125
            ++    + WG FQ +NHG+  E +   R+  ++FF +P+  K  YS      EGYG+ +
Sbjct: 83  ARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMPVNAKETYSNSPRTYEGYGSRL 142

Query: 126 VLSNEQILDWTDRLYLTVYPQQSRRSKYWPTNPERFREVVDEYTDNVRLISEKILKAMAR 185
            +     LDW+D  +L + P   +    WP+ P   REV+DEY + +  +S +I++ ++ 
Sbjct: 143 GVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTIREVIDEYGEELVKLSGRIMRVLST 202

Query: 186 SLDLDESSFLNQY-GERVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGL 245
           +L L E  F   + GE +    R N+YP+C  P+L LG+ PH+D   +TILL D +V GL
Sbjct: 203 NLGLKEDKFQEAFGGENIGACLRVNYYPKCPRPELALGLSPHSDPGGMTILLPDDQVFGL 262

Query: 246 QFMKGNEWFNAAIIPDALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDS 305
           Q  K + W      P A +VN+GDQ +I SN  +KS  HRV+ NS++ER+SLA FY P S
Sbjct: 263 QVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEHRVIVNSDKERVSLAFFYNPKS 322

Query: 306 EKEIEPLEKLINETQPRLY 317
           +  I+PL++L++   P LY
Sbjct: 323 DIPIQPLQELVSTHNPPLY 341

BLAST of HG10008099 vs. ExPASy Swiss-Prot
Match: D4N502 (Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.8e-54
Identity = 119/317 (37.54%), Postives = 174/317 (54.89%), Query Frame = 0

Query: 9   TVQDVA--SKGEVPERY-IHKESDRGALNAPLM---AAPVIDIA-LLSSSSKSGP-ELEK 68
           +VQ++A  +  E+P RY    ES    + A +      PVID+  LLS     G  EL+K
Sbjct: 17  SVQELAKLTLAEIPSRYTCTGESPLNNIGASVTDDETVPVIDLQNLLSPEPVVGKLELDK 76

Query: 69  LRHGLQSWGCFQAINHGMSTEFLYEVRQLAKQFFGLPMKEKLKYSREEDEMEGYGNDMVL 128
           L    + WG FQ +NHG+    +  ++   K FF LPM EK KY +++ + EG+G   + 
Sbjct: 77  LHSACKEWGFFQLVNHGVDALLMDNIKSEIKGFFNLPMNEKTKYGQQDGDFEGFGQPYIE 136

Query: 129 SNEQILDWTDRLYLTVYPQQSRRSKYWPTNPERFREVVDEYTDNVRLISEKILKAMARSL 188
           S +Q LDWT+   +   P   R+   +P  P  FRE ++ Y   ++ +S  + + + +SL
Sbjct: 137 SEDQRLDWTEVFSMLSLPLHLRKPHLFPELPLPFRETLESYLSKMKKLSTVVFEMLEKSL 196

Query: 189 DLDESSFLNQYGERVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 248
            L E   +    E      R N+YP C  P+LVLG+  H+D S +TILLQ  EVEGLQ  
Sbjct: 197 QLVEIKGMTDLFEDGLQTMRMNYYPPCPRPELVLGLTSHSDFSGLTILLQLNEVEGLQIR 256

Query: 249 KGNEWFNAAIIPDALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 308
           K   W +   +PDA +VNVGD  EI +NGI++S  HR + NS +ER+S+A F+    E E
Sbjct: 257 KEERWISIKPLPDAFIVNVGDILEIMTNGIYRSVEHRAVVNSTKERLSIATFHDSKLESE 316

Query: 309 IEPLEKLINETQPRLYK 318
           I P+  L+    P L+K
Sbjct: 317 IGPISSLVTPETPALFK 333

BLAST of HG10008099 vs. ExPASy TrEMBL
Match: A0A5A7U2M0 (Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00660 PE=3 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 2.2e-180
Identity = 313/342 (91.52%), Postives = 328/342 (95.91%), Query Frame = 0

Query: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIALLSSSSKSGPELEK 60
           MA TNPSGTVQDVASKGEVPERYIHKESDRGA NAPLMAAPVIDIALLSSSSKSGPELEK
Sbjct: 20  MASTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEK 79

Query: 61  LRHGLQSWGCFQAINHGMSTEFLYEVRQLAKQFFGLPMKEKLKYSREEDEMEGYGNDMVL 120
           LRHGLQSWGCFQAINHGM++E+L EVRQL KQFFGL M+EKLKY +EE EMEGYGNDM+L
Sbjct: 80  LRHGLQSWGCFQAINHGMTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMIL 139

Query: 121 SNEQILDWTDRLYLTVYPQQSRRSKYWPTNPERFREVVDEYTDNVRLISEKILKAMARSL 180
           SN+QILDWTDRLYLTVYP QSRR KYWPTNP+RFREVV EYT NV+LISEKILKAMARSL
Sbjct: 140 SNQQILDWTDRLYLTVYPHQSRRFKYWPTNPQRFREVVGEYTTNVKLISEKILKAMARSL 199

Query: 181 DLDESSFLNQYGERVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 240
           DLDESSFLNQYGE+VKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM
Sbjct: 200 DLDESSFLNQYGEQVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 259

Query: 241 KGNEWFNAAIIPDALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 300
           K NEW+NA I+PDALLVNVGDQ EITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE
Sbjct: 260 KDNEWYNAPIVPDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 319

Query: 301 IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 343
           IEPLE+LINE+QPRLYK+VKNFVGLYFEYYQQGQRPMEAARI
Sbjct: 320 IEPLEELINESQPRLYKSVKNFVGLYFEYYQQGQRPMEAARI 361

BLAST of HG10008099 vs. ExPASy TrEMBL
Match: A0A5D3CIZ6 (Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold367G00230 PE=3 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 2.1e-178
Identity = 310/342 (90.64%), Postives = 326/342 (95.32%), Query Frame = 0

Query: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIALLSSSSKSGPELEK 60
           MA TNPSGTVQDVASKGEVPERYIHKESDRGA NAPLMAAPVIDIALLSSSSKSGPELEK
Sbjct: 20  MASTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEK 79

Query: 61  LRHGLQSWGCFQAINHGMSTEFLYEVRQLAKQFFGLPMKEKLKYSREEDEMEGYGNDMVL 120
           LRHGLQSWGCFQAINHGM++E+L EVRQL KQFFGL M+EKLKY +EE EMEGYGNDM+L
Sbjct: 80  LRHGLQSWGCFQAINHGMTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMIL 139

Query: 121 SNEQILDWTDRLYLTVYPQQSRRSKYWPTNPERFREVVDEYTDNVRLISEKILKAMARSL 180
           SN+QILDWTDRLYLTVYP QSRR KYWP NP+RFREVV EYT NV+LISEKILKAMARSL
Sbjct: 140 SNQQILDWTDRLYLTVYPHQSRRFKYWPINPQRFREVVGEYTTNVKLISEKILKAMARSL 199

Query: 181 DLDESSFLNQYGERVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 240
           DLDESSFLNQYGE+VKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM
Sbjct: 200 DLDESSFLNQYGEQVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 259

Query: 241 KGNEWFNAAIIPDALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 300
           K NEW+NA I+ DALLVNVGDQ EITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE
Sbjct: 260 KDNEWYNAPIVTDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 319

Query: 301 IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 343
           IEPLE+LINE+QPRLYK+VKNFVGLYFEYYQQG+RPMEAARI
Sbjct: 320 IEPLEELINESQPRLYKSVKNFVGLYFEYYQQGERPMEAARI 361

BLAST of HG10008099 vs. ExPASy TrEMBL
Match: A0A1S3CP33 (protein SRG1-like OS=Cucumis melo OX=3656 GN=LOC103503160 PE=3 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 4.6e-178
Identity = 309/342 (90.35%), Postives = 327/342 (95.61%), Query Frame = 0

Query: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIALLSSSSKSGPELEK 60
           MA TNPSGTVQDVASKGEVPERYIHKESDRGA NAPLMAAPVIDIALLSSSSKSGPELEK
Sbjct: 20  MASTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEK 79

Query: 61  LRHGLQSWGCFQAINHGMSTEFLYEVRQLAKQFFGLPMKEKLKYSREEDEMEGYGNDMVL 120
           LRHGLQSWGCFQAINHGM++E+L EVRQL KQFFGL M+EKLKY +EE EMEGYGNDM+L
Sbjct: 80  LRHGLQSWGCFQAINHGMTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMIL 139

Query: 121 SNEQILDWTDRLYLTVYPQQSRRSKYWPTNPERFREVVDEYTDNVRLISEKILKAMARSL 180
           SN+QILDWTDRLYLTVYP QSRR KYWPTNP+RFREVV EYT NV+LISEKILKAMARSL
Sbjct: 140 SNQQILDWTDRLYLTVYPHQSRRFKYWPTNPQRFREVVGEYTTNVKLISEKILKAMARSL 199

Query: 181 DLDESSFLNQYGERVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 240
           DLDESSFL+QYGE+VKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDK+VEGLQFM
Sbjct: 200 DLDESSFLSQYGEQVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKKVEGLQFM 259

Query: 241 KGNEWFNAAIIPDALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 300
           K NEW+NA I+ DALLVNVGDQ EITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE
Sbjct: 260 KDNEWYNAPIVTDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 319

Query: 301 IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 343
           IEPLE+LINE+QPRLYK+VKNFVGLYFEYYQQG+RPMEAARI
Sbjct: 320 IEPLEELINESQPRLYKSVKNFVGLYFEYYQQGERPMEAARI 361

BLAST of HG10008099 vs. ExPASy TrEMBL
Match: A0A0A0LYX6 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G263950 PE=3 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 8.2e-175
Identity = 305/342 (89.18%), Postives = 324/342 (94.74%), Query Frame = 0

Query: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIALLSSSSKSGPELEK 60
           MAGTNPSGTVQDVASKGEV ERYIH ESDRGA NAPLMAAPVIDIALLSSSSKSGPELEK
Sbjct: 1   MAGTNPSGTVQDVASKGEVHERYIHIESDRGARNAPLMAAPVIDIALLSSSSKSGPELEK 60

Query: 61  LRHGLQSWGCFQAINHGMSTEFLYEVRQLAKQFFGLPMKEKLKYSREEDEMEGYGNDMVL 120
           LRHGLQSWGCFQAINHGM++E+L EVR+L KQFFGL M+EKLKY +EE EMEGYGNDM+L
Sbjct: 61  LRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGLSMEEKLKYLKEELEMEGYGNDMIL 120

Query: 121 SNEQILDWTDRLYLTVYPQQSRRSKYWPTNPERFREVVDEYTDNVRLISEKILKAMARSL 180
           SN+QILDWTDRLYLTVYP QSRR KYWPTN +RFREV+DEYT NV+LISEKI KAMARSL
Sbjct: 121 SNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFREVIDEYTTNVKLISEKIFKAMARSL 180

Query: 181 DLDESSFLNQYGERVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 240
           DLDESSFL QYGE++KL ARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM
Sbjct: 181 DLDESSFLKQYGEQIKLGARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 240

Query: 241 KGNEWFNAAIIPDALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 300
           K NEWFNA+I+PDALLVNVGDQ EITSNGIFKSPVHRVLTNSERERISLAVFYLPD+EKE
Sbjct: 241 KDNEWFNASIVPDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDAEKE 300

Query: 301 IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 343
           IEPLE+LI+ETQPRLYK+VKNFVGL FEYYQQGQRPMEAARI
Sbjct: 301 IEPLEELISETQPRLYKSVKNFVGLSFEYYQQGQRPMEAARI 342

BLAST of HG10008099 vs. ExPASy TrEMBL
Match: A0A6J1JKD6 (probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485215 PE=3 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.4e-174
Identity = 301/342 (88.01%), Postives = 324/342 (94.74%), Query Frame = 0

Query: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIALLSSSSKSGPELEK 60
           MAGTNPSGTVQDVASKGEVPERYIH ESDRGAL+APLMAAPVID+ALLSS SKSGPELEK
Sbjct: 1   MAGTNPSGTVQDVASKGEVPERYIHIESDRGALDAPLMAAPVIDMALLSSPSKSGPELEK 60

Query: 61  LRHGLQSWGCFQAINHGMSTEFLYEVRQLAKQFFGLPMKEKLKYSREEDEMEGYGNDMVL 120
           LRHGLQSWGCFQ +NHGMS EFL EVR+LAKQFF LPM+EK KYSREE+E+EGYGNDM+L
Sbjct: 61  LRHGLQSWGCFQVVNHGMSAEFLDEVRRLAKQFFDLPMEEKSKYSREENEIEGYGNDMIL 120

Query: 121 SNEQILDWTDRLYLTVYPQQSRRSKYWPTNPERFREVVDEYTDNVRLISEKILKAMARSL 180
           SN+QILDWTDRLYLTVYP++S R KYWPTNPERFREV+ EYT NV+L+SEKILKAMA SL
Sbjct: 121 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMAISL 180

Query: 181 DLDESSFLNQYGERVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 240
           DL+E SF+ QYGE VKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQD+EVEGLQF+
Sbjct: 181 DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL 240

Query: 241 KGNEWFNAAIIPDALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 300
            GNEWFNA I+P ALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE
Sbjct: 241 NGNEWFNAPIVPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 300

Query: 301 IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 343
           IEPLEKLI+ET+PRLYK+VKNFVGLYF+YYQQGQRPMEAARI
Sbjct: 301 IEPLEKLIDETRPRLYKSVKNFVGLYFQYYQQGQRPMEAARI 342

BLAST of HG10008099 vs. TAIR 10
Match: AT5G20400.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 397.9 bits (1021), Expect = 8.2e-111
Identity = 192/341 (56.30%), Postives = 254/341 (74.49%), Query Frame = 0

Query: 9   TVQDVASKGE-VPERYIHKESDRG---ALNA--PLMAAPVIDIALLSSSSKSG-PELEKL 68
           TVQ+V + GE +PERY+H  +  G    LNA  P M  P ID+ LL SSS++G  EL KL
Sbjct: 8   TVQEVVAAGEGLPERYLHAPTGDGEVQPLNAAVPEMDIPAIDLNLLLSSSEAGQQELSKL 67

Query: 69  RHGLQSWGCFQAINHGMSTEFLYEVRQLAKQFFGLPMKEKLKYSREEDEMEGYGNDMVLS 128
              L +WG  Q +NHG++  FL ++ +L K+FF LP +EK K +RE D ++GYGNDM+L 
Sbjct: 68  HSALSTWGVVQVMNHGITKAFLDKIYKLTKEFFALPTEEKQKCAREIDSIQGYGNDMILW 127

Query: 129 NEQILDWTDRLYLTVYPQQSRRSKYWPTNPERFREVVDEYTDNVRLISEKILKAMARSLD 188
           ++Q+LDW DRLY+T YP+  R+  +WP  P  FRE + EYT   R++ E+  KAMARSL+
Sbjct: 128 DDQVLDWIDRLYITTYPEDQRQLNFWPEVPLGFRETLHEYTMKQRIVIEQFFKAMARSLE 187

Query: 189 LDESSFLNQYGERVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFMK 248
           L+E+SFL+ YGE   LD RFN YP C +PD V+GVKPHADGSAIT+LL DK+V GLQF K
Sbjct: 188 LEENSFLDMYGESATLDTRFNMYPPCPSPDKVIGVKPHADGSAITLLLPDKDVGGLQFQK 247

Query: 249 GNEWFNAAIIPDALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKEI 308
             +W+ A I+PD +L+NVGDQ EI SNGI+KSPVHRV+TN E+ERIS+A F +P ++KEI
Sbjct: 248 DGKWYKAPIVPDTILINVGDQMEIMSNGIYKSPVHRVVTNREKERISVATFCIPGADKEI 307

Query: 309 EPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 343
           +P+ +L++E +PRLYKTVK +V LYF+YYQQG+RP+EAA I
Sbjct: 308 QPVNELVSEARPRLYKTVKKYVELYFKYYQQGRRPIEAALI 348

BLAST of HG10008099 vs. TAIR 10
Match: AT1G49390.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 382.1 bits (980), Expect = 4.7e-106
Identity = 183/341 (53.67%), Postives = 247/341 (72.43%), Query Frame = 0

Query: 9   TVQDVASKGE-VPERYIHKESDRG---ALN--APLMAAPVIDIALLSSSSKSG-PELEKL 68
           TVQ+V + G+ +PERY+H  +  G    LN   P M  P ID++LL SSS  G  E++KL
Sbjct: 8   TVQEVVAAGQGLPERYLHAPTGEGESQPLNGAVPEMDIPAIDLSLLFSSSVDGQEEMKKL 67

Query: 69  RHGLQSWGCFQAINHGMSTEFLYEVRQLAKQFFGLPMKEKLKYSREEDEMEGYGNDMVLS 128
              L +WG  Q +NHG++  FL ++ +L KQFF LP +EK K +RE   ++GYGNDM+LS
Sbjct: 68  HSALSTWGVVQVMNHGITEAFLDKIYKLTKQFFALPTEEKHKCARETGNIQGYGNDMILS 127

Query: 129 NEQILDWTDRLYLTVYPQQSRRSKYWPTNPERFREVVDEYTDNVRLISEKILKAMARSLD 188
           + Q+LDW DRL+LT YP+  R+ K+WP  P  F E +DEYT   R++ EK  KAMARSL+
Sbjct: 128 DNQVLDWIDRLFLTTYPEDKRQLKFWPQVPVGFSETLDEYTMKQRVLIEKFFKAMARSLE 187

Query: 189 LDESSFLNQYGERVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFMK 248
           L+E+ FL  YGE   +++RFNF+P C  PD V+G+KPHADGSAIT+LL DK+VEGLQF+K
Sbjct: 188 LEENCFLEMYGENAVMNSRFNFFPPCPRPDKVIGIKPHADGSAITLLLPDKDVEGLQFLK 247

Query: 249 GNEWFNAAIIPDALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKEI 308
             +W+ A I+PD +L+ +GDQ EI SNGI+KSPVHRV+TN E+ERIS+A F +P  +KEI
Sbjct: 248 DGKWYKAPIVPDTILITLGDQMEIMSNGIYKSPVHRVVTNREKERISVATFCVPGLDKEI 307

Query: 309 EPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 343
            P + L+ E +PRLYKTV  +V L+++YYQQG+R +EAA I
Sbjct: 308 HPADGLVTEARPRLYKTVTKYVDLHYKYYQQGRRTIEAALI 348

BLAST of HG10008099 vs. TAIR 10
Match: AT5G54000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 367.5 bits (942), Expect = 1.2e-101
Identity = 175/342 (51.17%), Postives = 237/342 (69.30%), Query Frame = 0

Query: 9   TVQDVASKGE-VPERYIHKESDRGALNAPL------MAAPVIDIALLSSSSKSG-PELEK 68
           TVQ+V + GE +PERY++  +  G  + P       M   +ID+ LL SSS  G  EL K
Sbjct: 8   TVQEVVAAGEKLPERYLYTPTGDGEGDQPFNGLLPEMKISIIDLNLLFSSSDDGREELSK 67

Query: 69  LRHGLQSWGCFQAINHGMSTEFLYEVRQLAKQFFGLPMKEKLKYSREEDEMEGYGNDMVL 128
           L   + +WG  Q +NHG+S   L ++ +L KQFF LP KEK KY+RE    +G+GNDM+L
Sbjct: 68  LHSAISTWGVVQVMNHGISEALLDKIHELTKQFFVLPTKEKQKYAREISSFQGFGNDMIL 127

Query: 129 SNEQILDWTDRLYLTVYPQQSRRSKYWPTNPERFREVVDEYTDNVRLISEKILKAMARSL 188
           S++Q+LDW DRLYL  YP+  R+ K+WP NP  FRE + EYT   +L+ EK  KA+ARSL
Sbjct: 128 SDDQVLDWVDRLYLITYPEDQRQLKFWPENPSGFRETLHEYTMKQQLVVEKFFKALARSL 187

Query: 189 DLDESSFLNQYGERVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 248
           +L+++ FL  +GE   L+ RFN YP C  PD VLG+KPH+DGSA T++L DK VEGLQF+
Sbjct: 188 ELEDNCFLEMHGENATLETRFNIYPPCPRPDKVLGLKPHSDGSAFTLILPDKNVEGLQFL 247

Query: 249 KGNEWFNAAIIPDALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 308
           K  +W+ A+I+P  +L+NVGD  E+ SNGI+KSPVHRV+ N ++ERI +A F   D +KE
Sbjct: 248 KDGKWYKASILPHTILINVGDTMEVMSNGIYKSPVHRVVLNGKKERIYVATFCNADEDKE 307

Query: 309 IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 343
           I+PL  L++E +PRLYK VK     +F+YYQQG+RP+EAA I
Sbjct: 308 IQPLNGLVSEARPRLYKAVKKSEKNFFDYYQQGRRPIEAAMI 349

BLAST of HG10008099 vs. TAIR 10
Match: AT5G20550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 360.1 bits (923), Expect = 1.9e-99
Identity = 178/342 (52.05%), Postives = 242/342 (70.76%), Query Frame = 0

Query: 9   TVQDVASKGE-VPERYIHKES--DRGA-LNA--PLMAAPVIDIALLSSSSKSG-PELEKL 68
           TVQ+V + GE +PERY+   +  D G  LNA  P+M  P ID++LL S S  G  EL KL
Sbjct: 8   TVQEVVAAGEGIPERYLQPPAVDDNGQHLNAAVPVMDIPAIDLSLLLSPSDDGREELSKL 67

Query: 69  RHGLQSWGCFQAINHGMSTEFLYEVRQLAKQFFGLPMKEKLKYSREEDEMEGYGNDMVLS 128
              L +WG  Q INHG++   L ++ +L K+F  LP +EK KY+RE   ++GYGNDM+L 
Sbjct: 68  HSALSTWGVVQVINHGITKALLDKIYKLTKEFCALPSEEKQKYAREIGSIQGYGNDMILW 127

Query: 129 NEQILDWTDRLYLTVYPQQSRRSKYWPTNPERFREVVDEYTDNVRLISEKILKAMARSLD 188
           ++Q+LDW DRLY+T YP+  R+ K+WP  P  FRE + EYT    L+  ++ KAMA SL+
Sbjct: 128 DDQVLDWIDRLYITTYPEDQRQLKFWPDVPVGFRETLHEYTMKQHLVFNQVFKAMAISLE 187

Query: 189 LDESSFLNQYGERVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFMK 248
           L+E+ FL+  GE   +D RFN YP C  PD V+GV+PHAD SA T+LL DK VEGLQF+K
Sbjct: 188 LEENCFLDMCGENATMDTRFNMYPPCPRPDKVIGVRPHADKSAFTLLLPDKNVEGLQFLK 247

Query: 249 GNEWFNAAII-PDALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 308
             +W+ A ++  D +L+NVGDQ EI SNGI+KSPVHRV+TN+E+ERIS+A F +P ++KE
Sbjct: 248 DGKWYKAPVVASDTILINVGDQMEIMSNGIYKSPVHRVVTNTEKERISVATFCIPGADKE 307

Query: 309 IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 343
           I+P++ L++E +PRLYK VKN+V L  +YY QGQRP+ A+ I
Sbjct: 308 IQPVDGLVSEARPRLYKPVKNYVDLLNKYYIQGQRPIAASLI 349

BLAST of HG10008099 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 262.3 bits (669), Expect = 5.4e-70
Identity = 138/354 (38.98%), Postives = 211/354 (59.60%), Query Frame = 0

Query: 8   GTVQDV-----ASKGEVPERYIHKESDRGALNAPLMA------APVIDIALLSSSSKSG- 67
           G + DV     +   +VPER+I +E +RG + + L         PVID++ LS       
Sbjct: 12  GKIDDVQELIKSKPNKVPERFIREEYERGVVVSSLKTHHLHHQIPVIDLSKLSKPDNDDF 71

Query: 68  -PELEKLRHGLQSWGCFQAINHGMSTEFLYEVRQLAKQFFGLPMKEKLKYSREEDEMEGY 127
             E+ KL    + WG FQ INHG+  E + ++ ++A +FF +P++EK KY  E   ++GY
Sbjct: 72  FFEILKLSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDMPLEEKKKYPMEPGTVQGY 131

Query: 128 GNDMVLSNEQILDWTDRLYLTVYPQQSRRSKYWPTNPERFREVVDEYTDNVRLISEKILK 187
           G   + S +Q LDW +   L V+P Q R  K WP+ P RF E ++ Y+  +R + +++LK
Sbjct: 132 GQAFIFSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPARFSESLEGYSKEIRELCKRLLK 191

Query: 188 AMARSLDLDESSFLNQYGERVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDK-E 247
            +A SL L E  F   +GE V+   R N+YP C +PDLVLG+ PH+DGSA+T+L Q K  
Sbjct: 192 YIAISLGLKEERFEEMFGEAVQA-VRMNYYPPCSSPDLVLGLSPHSDGSALTVLQQSKNS 251

Query: 248 VEGLQFMKGNEWFNAAIIPDALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFY 307
             GLQ +K N W     +P+AL++N+GD  E+ SNG +KS  HR +TN E+ER+++  FY
Sbjct: 252 CVGLQILKDNTWVPVKPLPNALVINIGDTIEVLSNGKYKSVEHRAVTNREKERLTIVTFY 311

Query: 308 LPDSEKEIEPLEKLI-NETQPRLYKTVKNFVGLYFEYY----QQGQRPMEAARI 343
            P+ E EIEP+ +L+ +ET P  Y++  +  G Y  +Y     QG++ ++ A+I
Sbjct: 312 APNYEVEIEPMSELVDDETNPCKYRSYNH--GDYSYHYVSNKLQGKKSLDFAKI 362

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038878494.12.4e-18194.15probable 2-oxoglutarate-dependent dioxygenase ANS [Benincasa hispida][more]
KAA0050082.14.6e-18091.52protein SRG1-like [Cucumis melo var. makuwa][more]
TYK10346.14.3e-17890.64protein SRG1-like [Cucumis melo var. makuwa][more]
XP_008465535.19.6e-17890.35PREDICTED: protein SRG1-like [Cucumis melo] >XP_016903407.1 PREDICTED: protein S... [more]
XP_011655688.24.8e-17790.06probable 2-oxoglutarate-dependent dioxygenase ANS [Cucumis sativus] >KAE8653027.... [more]
Match NameE-valueIdentityDescription
O804491.5e-6137.69Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1[more]
Q392242.1e-5834.55Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1[more]
Q94LP43.3e-5634.952-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947... [more]
Q9FFF61.6e-5535.74Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1[more]
D4N5021.8e-5437.54Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7U2M02.2e-18091.52Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00... [more]
A0A5D3CIZ62.1e-17890.64Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold367G00... [more]
A0A1S3CP334.6e-17890.35protein SRG1-like OS=Cucumis melo OX=3656 GN=LOC103503160 PE=3 SV=1[more]
A0A0A0LYX68.2e-17589.18Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G... [more]
A0A6J1JKD62.4e-17488.01probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 OS=Cucurbita maxima... [more]
Match NameE-valueIdentityDescription
AT5G20400.18.2e-11156.302-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G49390.14.7e-10653.672-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G54000.11.2e-10151.172-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G20550.11.9e-9952.052-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G21420.15.4e-7038.982-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 9..332
e-value: 1.6E-91
score: 309.0
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 41..149
e-value: 1.9E-23
score: 83.4
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 201..294
e-value: 9.3E-21
score: 74.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availablePANTHERPTHR47991:SF632-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEINcoord: 9..335
NoneNo IPR availablePANTHERPTHR47991OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASEcoord: 9..335
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 14..320
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 195..295
score: 11.952472

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10008099.1HG10008099.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding