HG10008061 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10008061
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionmitogen-activated protein kinase kinase kinase YODA-like
LocationChr10: 19223415 .. 19224413 (-)
RNA-Seq ExpressionHG10008061
SyntenyHG10008061
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAAAGTCCTGTGTTGTTTCACAAGTGAATCAACAAAGCCACAACTTTGTTAACTTGCACGAATTCTTGAAAACAAAGAAACGCATTCTGAAGATTGCAAAGAAATCGATACCCAACAACAATTGGAAAGACAACGTCAAACACAATTCCGGAAACTTCGGAAACAATTTCGCTATCGAAGAATACCATGGTTCGATTTGGATTGGGGATCGTCTCTTTCGCAAAACAGGATCCAGCTTAATTTTCTTGGCCAAGAACAGAGAACCCATCAACCCAAATTCAAATCTTCCTACAGAGTTCGTTGTGAAATCTTGTTTAATGGAATCCTCTTCTTCACTCCAACATGAGAAGGAAGTGTTATTCAATCTAGGACCATACATTAATCTAGTGCAATGTTATGGAGATGAAATAACAACGACGAAATCAGGTGAAAAGATTTATAATGTCTTGTTTGAGTATTGCTCTGGGTCTAGTTTGTTTGATCACATTCTCAAATTTGGGCCTAATGGGCTGCCAGAACAGGAAGTTCGAAGGTATACAAGAGATATTGTACATGGACTTTATTACATGCATTGTAATGCTTGGTATATTCATGGAGACATAAAATCAAGAAACATTTTATTGTCACACGGTACGGCTAAGTTAGCTAGTTTTGGATTGGCTAGGAAGCTCACTGCTGAAGTATTGTGTGAGGAGGAAATTAGCGGTTCGGGATCGTATATGTCTCCGGAGTTGGCTCGAGATGGTTATTTAAGTTGGCCGGCTGATATTTGGGCACTTGGATGCGTCGTGTTGGAGATGTTGACGGGAAAACCTGCATGGAGCTTTGAAAGTGCACATAAATGTTTAGTGGATTGCAACAATGAAGAAGTGCCAGAAATTCCTAATGACATATCAAGGGAAGGAAGAGATTTTATTAAGAAGTGTTTGATTAGAAGTCCTTATAAAAGAAGACCAATTTGGTTGTTGATTAAACATCCTTTTGTTTGCTTTTGA

mRNA sequence

ATGACAAAGTCCTGTGTTGTTTCACAAGTGAATCAACAAAGCCACAACTTTGTTAACTTGCACGAATTCTTGAAAACAAAGAAACGCATTCTGAAGATTGCAAAGAAATCGATACCCAACAACAATTGGAAAGACAACGTCAAACACAATTCCGGAAACTTCGGAAACAATTTCGCTATCGAAGAATACCATGGTTCGATTTGGATTGGGGATCGTCTCTTTCGCAAAACAGGATCCAGCTTAATTTTCTTGGCCAAGAACAGAGAACCCATCAACCCAAATTCAAATCTTCCTACAGAGTTCGTTGTGAAATCTTGTTTAATGGAATCCTCTTCTTCACTCCAACATGAGAAGGAAGTGTTATTCAATCTAGGACCATACATTAATCTAGTGCAATGTTATGGAGATGAAATAACAACGACGAAATCAGGTGAAAAGATTTATAATGTCTTGTTTGAGTATTGCTCTGGGTCTAGTTTGTTTGATCACATTCTCAAATTTGGGCCTAATGGGCTGCCAGAACAGGAAGTTCGAAGGTATACAAGAGATATTGTACATGGACTTTATTACATGCATTGTAATGCTTGGTATATTCATGGAGACATAAAATCAAGAAACATTTTATTGTCACACGGTACGGCTAAGTTAGCTAGTTTTGGATTGGCTAGGAAGCTCACTGCTGAAGTATTGTGTGAGGAGGAAATTAGCGGTTCGGGATCGTATATGTCTCCGGAGTTGGCTCGAGATGGTTATTTAAGTTGGCCGGCTGATATTTGGGCACTTGGATGCGTCGTGTTGGAGATGTTGACGGGAAAACCTGCATGGAGCTTTGAAAGTGCACATAAATGTTTAGTGGATTGCAACAATGAAGAAGTGCCAGAAATTCCTAATGACATATCAAGGGAAGGAAGAGATTTTATTAAGAAGTGTTTGATTAGAAGTCCTTATAAAAGAAGACCAATTTGGTTGTTGATTAAACATCCTTTTGTTTGCTTTTGA

Coding sequence (CDS)

ATGACAAAGTCCTGTGTTGTTTCACAAGTGAATCAACAAAGCCACAACTTTGTTAACTTGCACGAATTCTTGAAAACAAAGAAACGCATTCTGAAGATTGCAAAGAAATCGATACCCAACAACAATTGGAAAGACAACGTCAAACACAATTCCGGAAACTTCGGAAACAATTTCGCTATCGAAGAATACCATGGTTCGATTTGGATTGGGGATCGTCTCTTTCGCAAAACAGGATCCAGCTTAATTTTCTTGGCCAAGAACAGAGAACCCATCAACCCAAATTCAAATCTTCCTACAGAGTTCGTTGTGAAATCTTGTTTAATGGAATCCTCTTCTTCACTCCAACATGAGAAGGAAGTGTTATTCAATCTAGGACCATACATTAATCTAGTGCAATGTTATGGAGATGAAATAACAACGACGAAATCAGGTGAAAAGATTTATAATGTCTTGTTTGAGTATTGCTCTGGGTCTAGTTTGTTTGATCACATTCTCAAATTTGGGCCTAATGGGCTGCCAGAACAGGAAGTTCGAAGGTATACAAGAGATATTGTACATGGACTTTATTACATGCATTGTAATGCTTGGTATATTCATGGAGACATAAAATCAAGAAACATTTTATTGTCACACGGTACGGCTAAGTTAGCTAGTTTTGGATTGGCTAGGAAGCTCACTGCTGAAGTATTGTGTGAGGAGGAAATTAGCGGTTCGGGATCGTATATGTCTCCGGAGTTGGCTCGAGATGGTTATTTAAGTTGGCCGGCTGATATTTGGGCACTTGGATGCGTCGTGTTGGAGATGTTGACGGGAAAACCTGCATGGAGCTTTGAAAGTGCACATAAATGTTTAGTGGATTGCAACAATGAAGAAGTGCCAGAAATTCCTAATGACATATCAAGGGAAGGAAGAGATTTTATTAAGAAGTGTTTGATTAGAAGTCCTTATAAAAGAAGACCAATTTGGTTGTTGATTAAACATCCTTTTGTTTGCTTTTGA

Protein sequence

MTKSCVVSQVNQQSHNFVNLHEFLKTKKRILKIAKKSIPNNNWKDNVKHNSGNFGNNFAIEEYHGSIWIGDRLFRKTGSSLIFLAKNREPINPNSNLPTEFVVKSCLMESSSSLQHEKEVLFNLGPYINLVQCYGDEITTTKSGEKIYNVLFEYCSGSSLFDHILKFGPNGLPEQEVRRYTRDIVHGLYYMHCNAWYIHGDIKSRNILLSHGTAKLASFGLARKLTAEVLCEEEISGSGSYMSPELARDGYLSWPADIWALGCVVLEMLTGKPAWSFESAHKCLVDCNNEEVPEIPNDISREGRDFIKKCLIRSPYKRRPIWLLIKHPFVCF
Homology
BLAST of HG10008061 vs. NCBI nr
Match: XP_004147483.2 (mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN59621.1 hypothetical protein Csa_001780 [Cucumis sativus])

HSP 1 Score: 542.7 bits (1397), Expect = 2.2e-150
Identity = 264/333 (79.28%), Postives = 285/333 (85.59%), Query Frame = 0

Query: 1   MTKSCVVSQVNQQSHNFVNLHEFLKTKKRILKIAKKSIPNNNWKDNVKHNSGNFGNNFAI 60
           MTKSCVVSQV        NLHEFLKTK+RILKIAKKS P NNW D +KHN   FG +F I
Sbjct: 1   MTKSCVVSQVQISG----NLHEFLKTKRRILKIAKKSAPRNNWNDTIKHNYEKFGEDFTI 60

Query: 61  EEYHGSIWIGDRLFRKTGSSLIFLA-KNREPI-NPNSNLPTEFVVKSCLMESSSSLQHEK 120
           EE+HGSIW G  LFRKT S+L+FLA KNR PI N NSNLP EF+VKS LMESSSSL+HEK
Sbjct: 61  EEFHGSIWTGSLLFRKTESTLVFLAKKNRAPINNSNSNLPEEFLVKSSLMESSSSLRHEK 120

Query: 121 EVLFNLGPYINLVQCYGDEITTTKSGEKIYNVLFEYCSGSSLFDHILKFGPNGLPEQEVR 180
           EVL NLGP+ NLV CYGDEIT TKSGE++YNV FEYCSGSSL +HILKFGPNGL + EVR
Sbjct: 121 EVLSNLGPFTNLVDCYGDEITVTKSGEEVYNVFFEYCSGSSLRNHILKFGPNGLQDDEVR 180

Query: 181 RYTRDIVHGLYYMHCNAWYIHGDIKSRNILLSHGTAKLASFGLARKLTAEVLCEEEISGS 240
           RYTRDIV GLYYMHCN  YIHGDIKSRNILLSHG AKLASFGLARKLTAEV+CEEEISGS
Sbjct: 181 RYTRDIVRGLYYMHCNGRYIHGDIKSRNILLSHGMAKLASFGLARKLTAEVICEEEISGS 240

Query: 241 GSYMSPELARDGYLSWPADIWALGCVVLEMLTGKPAWSFESAHKCLVDCNNEEVPEIPND 300
           G Y SPELAR+GYL WPADIWALGCVVLEM TGK AWSFE A++ L+D NNE++PEIP +
Sbjct: 241 GPYASPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFEDAYRYLMDSNNEKIPEIPKN 300

Query: 301 ISREGRDFIKKCLIRSPYKRRPIWLLIKHPFVC 332
           ISREGRDFIKKCLIRSPYKRRPIWLLIKHPFVC
Sbjct: 301 ISREGRDFIKKCLIRSPYKRRPIWLLIKHPFVC 329

BLAST of HG10008061 vs. NCBI nr
Match: XP_008443462.1 (PREDICTED: mitogen-activated protein kinase kinase kinase YODA-like [Cucumis melo] >KAA0053712.1 mitogen-activated protein kinase kinase kinase YODA-like [Cucumis melo var. makuwa] >TYK17746.1 mitogen-activated protein kinase kinase kinase YODA-like [Cucumis melo var. makuwa])

HSP 1 Score: 540.8 bits (1392), Expect = 8.2e-150
Identity = 264/333 (79.28%), Postives = 283/333 (84.98%), Query Frame = 0

Query: 1   MTKSCVVSQVNQQSHNFVNLHEFLKTKKRILKIAKKSIPNNNWKDNVKHNSGNFGNNFAI 60
           MTKSCVVS+V       VNLHEFLKTK+RILKIAKKS P NNW D +KHN   FG +F I
Sbjct: 1   MTKSCVVSRV----QFCVNLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTI 60

Query: 61  EEYHGSIWIGDRLFRKTGSSLIFLA-KNREPI-NPNSNLPTEFVVKSCLMESSSSLQHEK 120
           EE+HGSIW G  LFRKT S+LIFLA KNR PI N  SNLP EF+VKS L ESSSSL HEK
Sbjct: 61  EEFHGSIWSGVHLFRKTESTLIFLAKKNRAPINNSTSNLPEEFLVKSSLTESSSSLHHEK 120

Query: 121 EVLFNLGPYINLVQCYGDEITTTKSGEKIYNVLFEYCSGSSLFDHILKFGPNGLPEQEVR 180
           EVL NLGP++NLV CYGDEIT +KSGEK+YNV FEYCSGSSL DHI KFGPNGL E EVR
Sbjct: 121 EVLSNLGPFMNLVDCYGDEITISKSGEKVYNVFFEYCSGSSLRDHIRKFGPNGLQEHEVR 180

Query: 181 RYTRDIVHGLYYMHCNAWYIHGDIKSRNILLSHGTAKLASFGLARKLTAEVLCEEEISGS 240
           +YTRDIVHGLYYMHCN  YIHGDIKSRNILLSHG AKLASFGLARKLTAEV+CEEEISGS
Sbjct: 181 KYTRDIVHGLYYMHCNGRYIHGDIKSRNILLSHGMAKLASFGLARKLTAEVICEEEISGS 240

Query: 241 GSYMSPELARDGYLSWPADIWALGCVVLEMLTGKPAWSFESAHKCLVDCNNEEVPEIPND 300
           G Y SPELAR+GYL WPADIWALGCVVLEM TGK AWSF  AH+ L+DCNNE++PEIP +
Sbjct: 241 GPYASPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFGDAHRYLMDCNNEKIPEIPKN 300

Query: 301 ISREGRDFIKKCLIRSPYKRRPIWLLIKHPFVC 332
           ISREGRDFIKKCLIRSPYKRRP WLLIKHPFVC
Sbjct: 301 ISREGRDFIKKCLIRSPYKRRPSWLLIKHPFVC 329

BLAST of HG10008061 vs. NCBI nr
Match: XP_038880300.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 540.0 bits (1390), Expect = 1.4e-149
Identity = 265/331 (80.06%), Postives = 281/331 (84.89%), Query Frame = 0

Query: 1   MTKSCVVSQVNQQSHNFVNLHEFLKTKKRILKIAKKSIPNNNWKDNVKHNSGNFGNNFAI 60
           MTKSCVVSQVNQ+SHNFVNLHEFLKTK+ I+K AKKS+  NNW DNVKHNS  FG +FAI
Sbjct: 1   MTKSCVVSQVNQRSHNFVNLHEFLKTKRGIVKCAKKSLRTNNWNDNVKHNSEKFGKDFAI 60

Query: 61  EEYHGSIWIGDRLFRKTGSSLIFLAKNREPINPNSNLPTEFVVKSCLMESSSSLQHEKEV 120
           EEYHGSIW GD LFRKTGSSLIFLA+NR P NPNSNLPTEF+VKSCLMESSSSLQHEKEV
Sbjct: 61  EEYHGSIWTGDHLFRKTGSSLIFLARNRAPTNPNSNLPTEFMVKSCLMESSSSLQHEKEV 120

Query: 121 LFNLGPYINLVQCYGDEITTTKSGEKIYNVLFEYCSGSSLFDHILKFGPNGLPEQEVRRY 180
           L NLG + NLV+CYGDEIT TKSGEK+YNVLFEYC GSSL DHILKFGPNGLPE EVRRY
Sbjct: 121 LNNLGSFTNLVRCYGDEITITKSGEKVYNVLFEYCYGSSLRDHILKFGPNGLPEHEVRRY 180

Query: 181 TRDIVHGLYYMHCNAWYIHGDIKSRNILLSHGTAKLASFGLARKLTAEVLCEEEISGSGS 240
           TRDIV+G+ YMHCN  YIHGDIKSRNIL+S   AKLASFG ARKLTAEVLCEEEI GSG 
Sbjct: 181 TRDIVYGVCYMHCNGRYIHGDIKSRNILMSPTGAKLASFGWARKLTAEVLCEEEIRGSGP 240

Query: 241 YMSPELARDGYLSWPADIWALGCVVLEMLTGKPAWSFESAHKCLVDCNNEEVPEIPNDIS 300
           Y+SPELA DGYL WPADIWALGCVVLEMLTGK               NNEE  +I NDIS
Sbjct: 241 YISPELASDGYLGWPADIWALGCVVLEMLTGKS------------PRNNEEAIQIINDIS 300

Query: 301 REGRDFIKKCLIRSPYKRRPIWLLIKHPFVC 332
           +EGRDFIKKCLI SPYKRRP WLLIKHPFVC
Sbjct: 301 KEGRDFIKKCLITSPYKRRPSWLLIKHPFVC 319

BLAST of HG10008061 vs. NCBI nr
Match: XP_023516554.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 522.7 bits (1345), Expect = 2.3e-144
Identity = 257/337 (76.26%), Postives = 283/337 (83.98%), Query Frame = 0

Query: 1   MTKSCVVSQVNQQSHNFVNLHEFLKTKKRILKIAKKSIPNNNWKDNVKHNSGNFGNNFAI 60
           MTKSCVVSQVNQQ HN VNLHEFLKTK+ I+K AKKS  +NNW +NVKHN G FG NFAI
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSNNWNNNVKHNYGRFG-NFAI 60

Query: 61  EEYHGSIWIGDRLFRKTGSSLIFLAKNREPINPNSNLPTEFVVKSCLMESSSSLQHEKEV 120
           EEYHGS WIGDRL RK+GS L+FLAKN+   N N     EF+VKSCLMESSSSLQ+EKEV
Sbjct: 61  EEYHGSFWIGDRLIRKSGSGLVFLAKNKSSTNSN----PEFLVKSCLMESSSSLQYEKEV 120

Query: 121 LFNLGPYINLVQCYGDEITTTKSGEKIYNVLFEYCSGSSLFDHILKFGPNGLPEQEVRRY 180
           L NLGP  NLV+CYGDEIT  KSGE+IYNV+FEYC GSSL DHI+KFG NGLPE  VR+Y
Sbjct: 121 LRNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKFGRNGLPEPIVRQY 180

Query: 181 TRDIVHGLYYMHCNAWYIHGDIKSRNILLSHG-------TAKLASFGLARKLTAEVLCEE 240
           TRDIVHGL YMHCNA YIHGDIK RNILLS          AKLASFGLARKLT EVLC+E
Sbjct: 181 TRDIVHGLCYMHCNAGYIHGDIKPRNILLSPSDHQGGCLVAKLASFGLARKLTPEVLCDE 240

Query: 241 EISGSGSYMSPELARDGYLSWPADIWALGCVVLEMLTGKPAWSFESAHKCLVDCNNEEVP 300
           EISGSGSY++PELA+DGYL WPADIWALGCVVLEMLTGKPAWSFESA+  L+DC N+E+P
Sbjct: 241 EISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMDC-NQEIP 300

Query: 301 EIPNDISREGRDFIKKCLIRSPYKRRPIWLLIKHPFV 331
           EIP+ IS  GRDF+K+CLIRSPYKRRP WLL+KHPFV
Sbjct: 301 EIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 331

BLAST of HG10008061 vs. NCBI nr
Match: KAG7023508.1 (Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 518.1 bits (1333), Expect = 5.7e-143
Identity = 255/337 (75.67%), Postives = 282/337 (83.68%), Query Frame = 0

Query: 1   MTKSCVVSQVNQQSHNFVNLHEFLKTKKRILKIAKKSIPNNNWKDNVKHNSGNFGNNFAI 60
           MTKSCVVSQVNQQ HN VNLHEFLKTK+ I+K AKKS  + NW +NVKHN G FG NFAI
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSTNWNNNVKHNYGRFG-NFAI 60

Query: 61  EEYHGSIWIGDRLFRKTGSSLIFLAKNREPINPNSNLPTEFVVKSCLMESSSSLQHEKEV 120
           EEYHGS WIGDRL RK+GS L+FLAKN+   N NS    EF+VKSCLMESSSSLQ+EKEV
Sbjct: 61  EEYHGSFWIGDRLIRKSGSGLVFLAKNKSSTNSNS----EFLVKSCLMESSSSLQYEKEV 120

Query: 121 LFNLGPYINLVQCYGDEITTTKSGEKIYNVLFEYCSGSSLFDHILKFGPNGLPEQEVRRY 180
           L NLGP  NLV+CYGDEIT  KSGE+IYNV+FEYC GSSL DHI+KFG NGLPE  VR+Y
Sbjct: 121 LCNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIVRKY 180

Query: 181 TRDIVHGLYYMHCNAWYIHGDIKSRNILLSHG-------TAKLASFGLARKLTAEVLCEE 240
           TRDIV GL YMHCNA YIHGDIK RNILLS          AKLASFGLARKLT EVLC+E
Sbjct: 181 TRDIVRGLCYMHCNAGYIHGDIKPRNILLSPSDHKGGCLVAKLASFGLARKLTPEVLCDE 240

Query: 241 EISGSGSYMSPELARDGYLSWPADIWALGCVVLEMLTGKPAWSFESAHKCLVDCNNEEVP 300
           EISGSGSY++PELA+DGYL WPADIWALGCVVLEMLTGKPAWSFESA+  L++C N+E+P
Sbjct: 241 EISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMNC-NQEIP 300

Query: 301 EIPNDISREGRDFIKKCLIRSPYKRRPIWLLIKHPFV 331
           EIP+ IS  GRDF+K+CLIRSPYKRRP WLL+KHPFV
Sbjct: 301 EIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 331

BLAST of HG10008061 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 3.3e-45
Identity = 103/272 (37.87%), Postives = 159/272 (58.46%), Query Frame = 0

Query: 68  WI-GDRLFRKTGSSLIFLAKNREPINPNSNLPTEFVVKSCLMESSSSLQHEKEVLFNLGP 127
           W+ G+ +   T S++    K+R     + + P    VKS     ++SL +EK VL +LG 
Sbjct: 3   WVRGETIGFGTFSTVSTATKSRN----SGDFPALIAVKSTDAYGAASLSNEKSVLDSLGD 62

Query: 128 YINLVQCYGDEITTTKSGEKIYNVLFEYCSGSSLFDHILKFGPNGLPEQEVRRYTRDIVH 187
              +++CYG++ +T ++GE+++N+L EY S  SL  ++ K G  GLPE  VRR+T  ++ 
Sbjct: 63  CPEIIRCYGED-STVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLR 122

Query: 188 GLYYMHCNAWYIHGDIKSRNILL-SHGTAKLASFGLARKLTAEVLC---EEEISGSGSYM 247
           GL ++H    + H DIK  NILL + G+ K+A FGLA ++  ++       EI G+  YM
Sbjct: 123 GLRHIHAKG-FAHCDIKLANILLFNDGSVKIADFGLAMRVDGDLTALRKSVEIRGTPLYM 182

Query: 248 SPELARDGYLSWPADIWALGCVVLEMLTGKPAWSF-ESAH--KCLVDCN-NEEVPEIPND 307
           +PE   D      AD+WALGC V+EM +GK AWS  E +H    L+     +E+P+IP  
Sbjct: 183 APECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEM 242

Query: 308 ISREGRDFIKKCLIRSPYKRRPIWLLIKHPFV 331
           +S EG+DF+ KC ++ P KR    +L+ H FV
Sbjct: 243 LSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFV 268

BLAST of HG10008061 vs. ExPASy Swiss-Prot
Match: Q9CAD5 (Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3702 GN=YDA PE=1 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 4.5e-34
Identity = 89/225 (39.56%), Postives = 129/225 (57.33%), Query Frame = 0

Query: 109 ESSSSLQHEKEVLFNLGPYINLVQCYGDEITTTKSGEKIYNVLFEYCSGSSLFDHILKFG 168
           ES+  L  E  VL  L  + N+VQ YG E       +K+Y +  EY SG S++  + ++G
Sbjct: 442 ESAQQLGQEISVLSRL-RHQNIVQYYGSETV----DDKLY-IYLEYVSGGSIYKLLQEYG 501

Query: 169 PNGLPEQEVRRYTRDIVHGLYYMHCNAWYIHGDIKSRNILLS-HGTAKLASFGLARKLTA 228
             G  E  +R YT+ I+ GL Y+H     +H DIK  NIL+  HG  K+A FG+A+ +TA
Sbjct: 502 QFG--ENAIRNYTQQILSGLAYLHAKN-TVHRDIKGANILVDPHGRVKVADFGMAKHITA 561

Query: 229 EVLCEEEISGSGSYMSPELARDGYLS-WPADIWALGCVVLEMLTGKPAWS-FESAHKCLV 288
           +        GS  +M+PE+ ++   S    DIW+LGC VLEM T KP WS +E       
Sbjct: 562 Q-SGPLSFKGSPYWMAPEVIKNSNGSNLAVDIWSLGCTVLEMATTKPPWSQYEGVPAMFK 621

Query: 289 DCNNEEVPEIPNDISREGRDFIKKCLIRSPYKRRPIWLLIKHPFV 331
             N++E+P+IP+ +S EG+DF++KCL R+P  R     L+ H FV
Sbjct: 622 IGNSKELPDIPDHLSEEGKDFVRKCLQRNPANRPTAAQLLDHAFV 656

BLAST of HG10008061 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 3.8e-33
Identity = 86/270 (31.85%), Postives = 141/270 (52.22%), Query Frame = 0

Query: 68  WIGDRLFRKTGSSLIFLAKNREPINPNSNLPTEFVVKSCLMESSSSLQHEKEVLFNL-GP 127
           W   R+  +  ++ ++ A          N      VKS  +  S  LQ E ++L +L  P
Sbjct: 3   WTRGRILGRGSTATVYAAAGH-------NSDEILAVKSSEVHRSEFLQREAKILSSLSSP 62

Query: 128 YINLVQCYGDEITTTKSGEKIYNVLFEYCSGSSLFDHILKFGPNGLPEQEVRRYTRDIVH 187
           Y+  +   G E     +G  +YN+L EY    +L D   K G   + E  V +YTRDI+ 
Sbjct: 63  YV--IGYRGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDG-GRVDETRVVKYTRDILK 122

Query: 188 GLYYMHCNAWYIHGDIKSRNILLSH-GTAKLASFGLARKLTAEVLCEEEISGSGSYMSPE 247
           GL Y+H     +H D+K  N+++S  G AK+A FG A+++  + + E  + G+ ++M+PE
Sbjct: 123 GLEYIHSKG-IVHCDVKGSNVVISEKGEAKIADFGCAKRV--DPVFESPVMGTPAFMAPE 182

Query: 248 LARDGYLSWPADIWALGCVVLEMLTGKPAWSFESAHKCLVDC-----NNEEVPEIPNDIS 307
           +AR       +DIWA+GC ++EM+TG P W+   + +  V        + E PE+P  ++
Sbjct: 183 VARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCLLA 242

Query: 308 REGRDFIKKCLIRSPYKRRPIWLLIKHPFV 331
            E +DF++KCL R   +R     L+ HPF+
Sbjct: 243 EEAKDFLEKCLKREANERWTATQLLNHPFL 259

BLAST of HG10008061 vs. ExPASy Swiss-Prot
Match: F4HRJ4 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK3 PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.4e-30
Identity = 84/231 (36.36%), Postives = 128/231 (55.41%), Query Frame = 0

Query: 104 KSCLMESSSSLQHEKEVLFNLGPYINLVQCYGDEITTTKSGEKIYNVLFEYCSGSSLFDH 163
           K CL + +  +      L N   + N+VQ YG E++     E+  +V  EY SG S+   
Sbjct: 255 KECLKQLNQEIN-----LLNQLCHPNIVQYYGSELS-----EETLSVYLEYVSGGSIHKL 314

Query: 164 ILKFGPNGLPEQEVRRYTRDIVHGLYYMHCNAWYIHGDIKSRNILLS-HGTAKLASFGLA 223
           +  +G     E  ++ YTR I+ GL Y+H     +H DIK  NIL+  +G  KLA FG+A
Sbjct: 315 LKDYG--SFTEPVIQNYTRQILAGLAYLH-GRNTVHRDIKGANILVDPNGEIKLADFGMA 374

Query: 224 RKLTAEVLCEEEISGSGSYMSPE--LARDGYLSWPADIWALGCVVLEMLTGKPAWS-FES 283
           + +TA         GS  +M+PE  ++++GY +   DIW+LGC +LEM T KP WS FE 
Sbjct: 375 KHVTA-FSTMLSFKGSPYWMAPEVVMSQNGY-THAVDIWSLGCTILEMATSKPPWSQFEG 434

Query: 284 AHKCLVDCNNEEVPEIPNDISREGRDFIKKCLIRSPYKRRPIWLLIKHPFV 331
                   N+++ PEIP+ +S + ++FI+ CL R+P  R     L++HPF+
Sbjct: 435 VAAIFKIGNSKDTPEIPDHLSNDAKNFIRLCLQRNPTVRPTASQLLEHPFL 470

BLAST of HG10008061 vs. ExPASy Swiss-Prot
Match: Q40541 (Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097 GN=NPK1 PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.2e-29
Identity = 79/222 (35.59%), Postives = 123/222 (55.41%), Query Frame = 0

Query: 114 LQHEKEVLFNLGPYINLVQCYGDEITTTKSGEKIYNVLFEYCSGSSLFDHILKFGPNGLP 173
           L+ E  +L NL  + N+V+  G   T  ++G    N+L E+  G S+   + KFG    P
Sbjct: 130 LEEEVNLLKNLS-HPNIVRYLG---TAREAGS--LNILLEFVPGGSISSLLGKFG--SFP 189

Query: 174 EQEVRRYTRDIVHGLYYMHCNAWYIHGDIKSRNILLSH-GTAKLASFGLARKLT--AEVL 233
           E  +R YT+ ++ GL Y+H N   +H DIK  NIL+ + G  KLA FG ++K+   A + 
Sbjct: 190 ESVIRMYTKQLLLGLEYLHKNG-IMHRDIKGANILVDNKGCIKLADFGASKKVVELATMT 249

Query: 234 CEEEISGSGSYMSPELARDGYLSWPADIWALGCVVLEMLTGKPAWS--FESAHKCLVDCN 293
             + + G+  +M+PE+      S+ ADIW++GC ++EM TGKP WS  ++          
Sbjct: 250 GAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTIIEMATGKPPWSQQYQEVAALFHIGT 309

Query: 294 NEEVPEIPNDISREGRDFIKKCLIRSPYKRRPIWLLIKHPFV 331
            +  P IP  +S E +DF+ KCL + P+ R     L++HPFV
Sbjct: 310 TKSHPPIPEHLSAESKDFLLKCLQKEPHLRHSASNLLQHPFV 342

BLAST of HG10008061 vs. ExPASy TrEMBL
Match: A0A0A0LHV1 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G829110 PE=4 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 1.0e-150
Identity = 264/333 (79.28%), Postives = 285/333 (85.59%), Query Frame = 0

Query: 1   MTKSCVVSQVNQQSHNFVNLHEFLKTKKRILKIAKKSIPNNNWKDNVKHNSGNFGNNFAI 60
           MTKSCVVSQV        NLHEFLKTK+RILKIAKKS P NNW D +KHN   FG +F I
Sbjct: 1   MTKSCVVSQVQISG----NLHEFLKTKRRILKIAKKSAPRNNWNDTIKHNYEKFGEDFTI 60

Query: 61  EEYHGSIWIGDRLFRKTGSSLIFLA-KNREPI-NPNSNLPTEFVVKSCLMESSSSLQHEK 120
           EE+HGSIW G  LFRKT S+L+FLA KNR PI N NSNLP EF+VKS LMESSSSL+HEK
Sbjct: 61  EEFHGSIWTGSLLFRKTESTLVFLAKKNRAPINNSNSNLPEEFLVKSSLMESSSSLRHEK 120

Query: 121 EVLFNLGPYINLVQCYGDEITTTKSGEKIYNVLFEYCSGSSLFDHILKFGPNGLPEQEVR 180
           EVL NLGP+ NLV CYGDEIT TKSGE++YNV FEYCSGSSL +HILKFGPNGL + EVR
Sbjct: 121 EVLSNLGPFTNLVDCYGDEITVTKSGEEVYNVFFEYCSGSSLRNHILKFGPNGLQDDEVR 180

Query: 181 RYTRDIVHGLYYMHCNAWYIHGDIKSRNILLSHGTAKLASFGLARKLTAEVLCEEEISGS 240
           RYTRDIV GLYYMHCN  YIHGDIKSRNILLSHG AKLASFGLARKLTAEV+CEEEISGS
Sbjct: 181 RYTRDIVRGLYYMHCNGRYIHGDIKSRNILLSHGMAKLASFGLARKLTAEVICEEEISGS 240

Query: 241 GSYMSPELARDGYLSWPADIWALGCVVLEMLTGKPAWSFESAHKCLVDCNNEEVPEIPND 300
           G Y SPELAR+GYL WPADIWALGCVVLEM TGK AWSFE A++ L+D NNE++PEIP +
Sbjct: 241 GPYASPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFEDAYRYLMDSNNEKIPEIPKN 300

Query: 301 ISREGRDFIKKCLIRSPYKRRPIWLLIKHPFVC 332
           ISREGRDFIKKCLIRSPYKRRPIWLLIKHPFVC
Sbjct: 301 ISREGRDFIKKCLIRSPYKRRPIWLLIKHPFVC 329

BLAST of HG10008061 vs. ExPASy TrEMBL
Match: A0A5D3D3K9 (Mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1804G00170 PE=4 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 4.0e-150
Identity = 264/333 (79.28%), Postives = 283/333 (84.98%), Query Frame = 0

Query: 1   MTKSCVVSQVNQQSHNFVNLHEFLKTKKRILKIAKKSIPNNNWKDNVKHNSGNFGNNFAI 60
           MTKSCVVS+V       VNLHEFLKTK+RILKIAKKS P NNW D +KHN   FG +F I
Sbjct: 1   MTKSCVVSRV----QFCVNLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTI 60

Query: 61  EEYHGSIWIGDRLFRKTGSSLIFLA-KNREPI-NPNSNLPTEFVVKSCLMESSSSLQHEK 120
           EE+HGSIW G  LFRKT S+LIFLA KNR PI N  SNLP EF+VKS L ESSSSL HEK
Sbjct: 61  EEFHGSIWSGVHLFRKTESTLIFLAKKNRAPINNSTSNLPEEFLVKSSLTESSSSLHHEK 120

Query: 121 EVLFNLGPYINLVQCYGDEITTTKSGEKIYNVLFEYCSGSSLFDHILKFGPNGLPEQEVR 180
           EVL NLGP++NLV CYGDEIT +KSGEK+YNV FEYCSGSSL DHI KFGPNGL E EVR
Sbjct: 121 EVLSNLGPFMNLVDCYGDEITISKSGEKVYNVFFEYCSGSSLRDHIRKFGPNGLQEHEVR 180

Query: 181 RYTRDIVHGLYYMHCNAWYIHGDIKSRNILLSHGTAKLASFGLARKLTAEVLCEEEISGS 240
           +YTRDIVHGLYYMHCN  YIHGDIKSRNILLSHG AKLASFGLARKLTAEV+CEEEISGS
Sbjct: 181 KYTRDIVHGLYYMHCNGRYIHGDIKSRNILLSHGMAKLASFGLARKLTAEVICEEEISGS 240

Query: 241 GSYMSPELARDGYLSWPADIWALGCVVLEMLTGKPAWSFESAHKCLVDCNNEEVPEIPND 300
           G Y SPELAR+GYL WPADIWALGCVVLEM TGK AWSF  AH+ L+DCNNE++PEIP +
Sbjct: 241 GPYASPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFGDAHRYLMDCNNEKIPEIPKN 300

Query: 301 ISREGRDFIKKCLIRSPYKRRPIWLLIKHPFVC 332
           ISREGRDFIKKCLIRSPYKRRP WLLIKHPFVC
Sbjct: 301 ISREGRDFIKKCLIRSPYKRRPSWLLIKHPFVC 329

BLAST of HG10008061 vs. ExPASy TrEMBL
Match: A0A1S3B829 (mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo OX=3656 GN=LOC103487049 PE=4 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 4.0e-150
Identity = 264/333 (79.28%), Postives = 283/333 (84.98%), Query Frame = 0

Query: 1   MTKSCVVSQVNQQSHNFVNLHEFLKTKKRILKIAKKSIPNNNWKDNVKHNSGNFGNNFAI 60
           MTKSCVVS+V       VNLHEFLKTK+RILKIAKKS P NNW D +KHN   FG +F I
Sbjct: 1   MTKSCVVSRV----QFCVNLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTI 60

Query: 61  EEYHGSIWIGDRLFRKTGSSLIFLA-KNREPI-NPNSNLPTEFVVKSCLMESSSSLQHEK 120
           EE+HGSIW G  LFRKT S+LIFLA KNR PI N  SNLP EF+VKS L ESSSSL HEK
Sbjct: 61  EEFHGSIWSGVHLFRKTESTLIFLAKKNRAPINNSTSNLPEEFLVKSSLTESSSSLHHEK 120

Query: 121 EVLFNLGPYINLVQCYGDEITTTKSGEKIYNVLFEYCSGSSLFDHILKFGPNGLPEQEVR 180
           EVL NLGP++NLV CYGDEIT +KSGEK+YNV FEYCSGSSL DHI KFGPNGL E EVR
Sbjct: 121 EVLSNLGPFMNLVDCYGDEITISKSGEKVYNVFFEYCSGSSLRDHIRKFGPNGLQEHEVR 180

Query: 181 RYTRDIVHGLYYMHCNAWYIHGDIKSRNILLSHGTAKLASFGLARKLTAEVLCEEEISGS 240
           +YTRDIVHGLYYMHCN  YIHGDIKSRNILLSHG AKLASFGLARKLTAEV+CEEEISGS
Sbjct: 181 KYTRDIVHGLYYMHCNGRYIHGDIKSRNILLSHGMAKLASFGLARKLTAEVICEEEISGS 240

Query: 241 GSYMSPELARDGYLSWPADIWALGCVVLEMLTGKPAWSFESAHKCLVDCNNEEVPEIPND 300
           G Y SPELAR+GYL WPADIWALGCVVLEM TGK AWSF  AH+ L+DCNNE++PEIP +
Sbjct: 241 GPYASPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFGDAHRYLMDCNNEKIPEIPKN 300

Query: 301 ISREGRDFIKKCLIRSPYKRRPIWLLIKHPFVC 332
           ISREGRDFIKKCLIRSPYKRRP WLLIKHPFVC
Sbjct: 301 ISREGRDFIKKCLIRSPYKRRPSWLLIKHPFVC 329

BLAST of HG10008061 vs. ExPASy TrEMBL
Match: A0A6J1HBP5 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=3662 GN=LOC111461386 PE=4 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 5.2e-142
Identity = 253/337 (75.07%), Postives = 282/337 (83.68%), Query Frame = 0

Query: 1   MTKSCVVSQVNQQSHNFVNLHEFLKTKKRILKIAKKSIPNNNWKDNVKHNSGNFGNNFAI 60
           MTKSCVVSQVNQQ HN VNLHEFLKTK+ I+K AKKS  + NW +NVKHN G FG NFAI
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSTNWNNNVKHNYGRFG-NFAI 60

Query: 61  EEYHGSIWIGDRLFRKTGSSLIFLAKNREPINPNSNLPTEFVVKSCLMESSSSLQHEKEV 120
           EE HGS WIGDRL R++GS L+FLAKN+   N NS    EF+VKSCLMESSSSLQ+EKEV
Sbjct: 61  EECHGSFWIGDRLIRRSGSGLVFLAKNKSSTNSNS----EFLVKSCLMESSSSLQYEKEV 120

Query: 121 LFNLGPYINLVQCYGDEITTTKSGEKIYNVLFEYCSGSSLFDHILKFGPNGLPEQEVRRY 180
           L NLGP  NLV+CYGDEIT +KSGE+IYNV+FEYC GSSL DHI+KFG NGLPE  VR+Y
Sbjct: 121 LCNLGPCSNLVRCYGDEITMSKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIVRKY 180

Query: 181 TRDIVHGLYYMHCNAWYIHGDIKSRNILLSHG-------TAKLASFGLARKLTAEVLCEE 240
           TRDIV GL YMHCNA YIHGDIK RNILLS          AKLASFGLARKLT EVLC+E
Sbjct: 181 TRDIVRGLCYMHCNAGYIHGDIKPRNILLSPSDHKGGCLVAKLASFGLARKLTPEVLCDE 240

Query: 241 EISGSGSYMSPELARDGYLSWPADIWALGCVVLEMLTGKPAWSFESAHKCLVDCNNEEVP 300
           EISGSGSY++PELA+DGYL WPADIWALGCVVLEMLTGKPAWSFESA+  L++C N+E+P
Sbjct: 241 EISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMNC-NQEIP 300

Query: 301 EIPNDISREGRDFIKKCLIRSPYKRRPIWLLIKHPFV 331
           EIP+ IS  GRDF+K+CLIRSPYKRRP WLL+KHPFV
Sbjct: 301 EIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 331

BLAST of HG10008061 vs. ExPASy TrEMBL
Match: A0A6J1JAH8 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita maxima OX=3661 GN=LOC111485031 PE=4 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 2.2e-100
Identity = 180/230 (78.26%), Postives = 198/230 (86.09%), Query Frame = 0

Query: 108 MESSSSLQHEKEVLFNLGPYINLVQCYGDEITTTKSGEKIYNVLFEYCSGSSLFDHILKF 167
           MESSSSLQ+EKEVL NLGP  NLV+CYGDEIT  KSGE+IYNV+FEYC GSSL DHI+KF
Sbjct: 1   MESSSSLQYEKEVLCNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCCGSSLRDHIIKF 60

Query: 168 GPNGLPEQEVRRYTRDIVHGLYYMHCNAWYIHGDIKSRNILLSHG-------TAKLASFG 227
           G NGLPE  VR+YTRDIVHGL YMHCNA YIHGDIK RNILLS          AKLASFG
Sbjct: 61  GRNGLPEPIVRKYTRDIVHGLCYMHCNARYIHGDIKPRNILLSPSDHKGGCLVAKLASFG 120

Query: 228 LARKLTAEVLCEEEISGSGSYMSPELARDGYLSWPADIWALGCVVLEMLTGKPAWSFESA 287
           LARKLT EVLC+EEISGSGSY++PELA+DGYL WPADIWALGCVVLEMLTGKPAWSFESA
Sbjct: 121 LARKLTPEVLCDEEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESA 180

Query: 288 HKCLVDCNNEEVPEIPNDISREGRDFIKKCLIRSPYKRRPIWLLIKHPFV 331
           +  L+DC N+E+PEIP+ IS  GRDF+K+CLIRSPYKRRP WLL+KHPFV
Sbjct: 181 YNYLMDC-NQEIPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 229

BLAST of HG10008061 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 184.1 bits (466), Expect = 1.8e-46
Identity = 103/240 (42.92%), Postives = 148/240 (61.67%), Query Frame = 0

Query: 98  PTEFVVKSCLMESSSSLQHEKEVLFNLGPYINLVQCYGDEITTTKSGEKIYNVLFEYCSG 157
           P+   VKS  +  S++L++E++VL +LG    +V+C+G E  T ++GE+IYN+  EY SG
Sbjct: 31  PSLMAVKSSGVVCSAALRNERDVLDDLGDCSEIVRCFG-EGRTVENGEEIYNLFLEYASG 90

Query: 158 SSLFDHILKFGPNGLPEQEVRRYTRDIVHGLYYMHCNAWYIHGDIKSRNILL-SHGTAKL 217
            SL D I   G   LPE EVRR+TR IV GL ++H N  + H DIK  N+L+   G  K+
Sbjct: 91  GSLADRIKSSG-EALPEFEVRRFTRSIVKGLCHIHGNG-FTHCDIKLENVLVFGDGDVKI 150

Query: 218 ASFGLARKLTAEVLCEEEISGSGSYMSPELARDGYLSWPADIWALGCVVLEMLTGKPAWS 277
           + FGLA++ + EV    EI G+  YM+PE    G    PADIWALGC V+EM +GK AW 
Sbjct: 151 SDFGLAKRRSGEVCV--EIRGTPLYMAPESVNHGEFESPADIWALGCSVVEMSSGKTAWC 210

Query: 278 FE-----SAHKCLVDC-NNEEVPEIPNDISREGRDFIKKCLIRSPYKRRPIWLLIKHPFV 331
            E     +    LV   + +EVP IP ++S EG+DF+ KC +++  +R    +L+ HPF+
Sbjct: 211 LEDGVMNNVMSLLVRIGSGDEVPRIPVELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFL 265

BLAST of HG10008061 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 183.7 bits (465), Expect = 2.4e-46
Identity = 103/272 (37.87%), Postives = 159/272 (58.46%), Query Frame = 0

Query: 68  WI-GDRLFRKTGSSLIFLAKNREPINPNSNLPTEFVVKSCLMESSSSLQHEKEVLFNLGP 127
           W+ G+ +   T S++    K+R     + + P    VKS     ++SL +EK VL +LG 
Sbjct: 3   WVRGETIGFGTFSTVSTATKSRN----SGDFPALIAVKSTDAYGAASLSNEKSVLDSLGD 62

Query: 128 YINLVQCYGDEITTTKSGEKIYNVLFEYCSGSSLFDHILKFGPNGLPEQEVRRYTRDIVH 187
              +++CYG++ +T ++GE+++N+L EY S  SL  ++ K G  GLPE  VRR+T  ++ 
Sbjct: 63  CPEIIRCYGED-STVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLR 122

Query: 188 GLYYMHCNAWYIHGDIKSRNILL-SHGTAKLASFGLARKLTAEVLC---EEEISGSGSYM 247
           GL ++H    + H DIK  NILL + G+ K+A FGLA ++  ++       EI G+  YM
Sbjct: 123 GLRHIHAKG-FAHCDIKLANILLFNDGSVKIADFGLAMRVDGDLTALRKSVEIRGTPLYM 182

Query: 248 SPELARDGYLSWPADIWALGCVVLEMLTGKPAWSF-ESAH--KCLVDCN-NEEVPEIPND 307
           +PE   D      AD+WALGC V+EM +GK AWS  E +H    L+     +E+P+IP  
Sbjct: 183 APECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEM 242

Query: 308 ISREGRDFIKKCLIRSPYKRRPIWLLIKHPFV 331
           +S EG+DF+ KC ++ P KR    +L+ H FV
Sbjct: 243 LSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFV 268

BLAST of HG10008061 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 177.2 bits (448), Expect = 2.2e-44
Identity = 96/247 (38.87%), Postives = 142/247 (57.49%), Query Frame = 0

Query: 92  NPNSNLPTEFVVKSCLMESSSSLQHEKEVLFNLGPYIN-LVQCYGDEITTTKSGEKIYNV 151
           N +   P    VKS     ++SL +EK VL NLG   N +V+C+G++  T ++GE+++N+
Sbjct: 25  NDSGEFPPLMAVKSADSYGAASLANEKSVLDNLGDDCNEIVRCFGED-RTVENGEEMHNL 84

Query: 152 LFEYCSGSSLFDHILKFGPNGLPEQEVRRYTRDIVHGLYYMHCNAWYIHGDIKSRNILL- 211
             EY S  SL  ++ K    G+PE  VRR+T  ++ GL ++H N  + H D+K  NILL 
Sbjct: 85  FLEYASRGSLESYLKKLAGEGVPESTVRRHTGSVLRGLRHIHANG-FAHCDLKLGNILLF 144

Query: 212 SHGTAKLASFGLARKL--TAEVLCEEEISGSGSYMSPELARDGYLSWPADIWALGCVVLE 271
             G  K+A FGLA+++     +    +I G+  YM+PE   D       D+WALGCVV+E
Sbjct: 145 GDGAVKIADFGLAKRIGDLTALNYGVQIRGTPLYMAPESVNDNEYGSEGDVWALGCVVVE 204

Query: 272 MLTGKPAWSFESAHK----CLVDCNNEEVPEIPNDISREGRDFIKKCLIRSPYKRRPIWL 331
           M +GK AWS +         L     +EVP IP ++S +GRDF+ KC ++ P KR    +
Sbjct: 205 MFSGKTAWSLKEGSNFMSLLLRIGVGDEVPMIPEELSEQGRDFLSKCFVKDPKKRWTAEM 264

BLAST of HG10008061 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 157.5 bits (397), Expect = 1.8e-38
Identity = 94/234 (40.17%), Postives = 135/234 (57.69%), Query Frame = 0

Query: 101 FVVKSCLMESSSSLQHEKEVLFNL-GPYINLVQCYGDEITTTKSGEKI-YNVLFEYCSGS 160
           F VKS    SS+ LQ E+ +L  L  PYI  V+  G  +  TK  +K+ YN+L EY SG 
Sbjct: 31  FAVKSAEFSSSAFLQREQSILSKLSSPYI--VKYIGSNV--TKENDKLMYNLLMEYVSGG 90

Query: 161 SLFDHILKFGPNGLPEQEVRRYTRDIVHGLYYMHCNAWYIHGDIKSRNILLSHGTAKLAS 220
           SL D ++K     LPE  +R YTR I+ GL Y+H +   +H D+KS+N+++    AK+  
Sbjct: 91  SLHD-LIKNSGGKLPEPLIRSYTRQILKGLMYLH-DQGIVHCDVKSQNVMIGGEIAKIVD 150

Query: 221 FGLARKLTAEVLCEEEISGSGSYMSPELARDGYLSWPADIWALGCVVLEMLTGKPAW-SF 280
            G A+  T E     E SG+ ++MSPE+AR    S+PAD+WALGC V+EM TG   W   
Sbjct: 151 LGCAK--TVEENENLEFSGTPAFMSPEVARGEEQSFPADVWALGCTVIEMATGSSPWPEL 210

Query: 281 ESAHKCLVDCN-NEEVPEIPNDISREGRDFIKKCLIRSPYKRRPIWLLIKHPFV 331
                 +       E P IP  +S +G+DF++KCL + P +R  +  L++HPF+
Sbjct: 211 NDVVAAIYKIGFTGESPVIPVWLSEKGQDFLRKCLRKDPKQRWTVEELLQHPFL 256

BLAST of HG10008061 vs. TAIR 10
Match: AT3G45670.1 (Protein kinase superfamily protein )

HSP 1 Score: 155.6 bits (392), Expect = 6.9e-38
Identity = 105/324 (32.41%), Postives = 170/324 (52.47%), Query Frame = 0

Query: 24  LKTKKRILKIAKKSIPNNNWKDNVKHNSGNFGNNFAIEEYHGSIWIGDRLFRKTGSSLIF 83
           LK KK+ +  + +SI   ++K   + +S     +  +++   S W+  RL  +     ++
Sbjct: 55  LKKKKKSISSSVESIIGVSFKSASQKHSTTVTRDGGVKKI--SSWVKSRLLGEGAYGCVY 114

Query: 84  LAKNREPINPNSNLPTEFVVKSCLMESSSSLQHEKEVLFNL-GPYINLVQCYGDEITTTK 143
           LA +++ I       TE  +KS  +  + SL HE  +L +L  P++  ++CYG EI    
Sbjct: 115 LATSKDDI-----YKTERAIKSADVLKAWSLMHEGRILRSLQSPFV--IRCYGHEIAREG 174

Query: 144 SGEKIYNVLFEYCSGSSLFDHILKFGPNGLPEQEVRRYTRDIVHGLYYMHCNAWYIHGDI 203
           +G + YN++ EYCSG  L D +++    G+PE +V+++  D++ GL Y+H     IH +I
Sbjct: 175 TGHQ-YNLILEYCSGQCLAD-MIEDNQGGIPEFDVKQFAIDVLSGLSYIH-RRNIIHCEI 234

Query: 204 KSRNILLSHGTAKLASFGLARKLTAEVLCEEE-----------ISGSGSYMSPELARDGY 263
           K  N+LLS    +  S G   K+    L  E+           + G+  YM+PEL   G 
Sbjct: 235 KPDNLLLSPVDHRFRSNGFLTKIADFGLSMEKGSKEYGNGRGHMRGTTRYMAPELIGGGL 294

Query: 264 LSWPADIWALGCVVLEMLTGKPAWS--FESAHKCLVDC--NNEEVPEIPNDISREGRDFI 323
           L +  DI A GC VLEMLTGK  W    + AH   VD   +++  P+I   +S E +DF+
Sbjct: 295 LDFAVDICAFGCSVLEMLTGKRVWGEYGDLAHDDWVDLIGHSDLTPQISIRLSAEAQDFL 354

Query: 324 KKCLIRSPYKRRPIWLLIKHPFVC 332
            +CL++ P  R  I  L+ HPF+C
Sbjct: 355 MRCLVKEPGSRWTIGELVDHPFLC 366

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004147483.22.2e-15079.28mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN59621.1 ... [more]
XP_008443462.18.2e-15079.28PREDICTED: mitogen-activated protein kinase kinase kinase YODA-like [Cucumis mel... [more]
XP_038880300.11.4e-14980.06mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida][more]
XP_023516554.12.3e-14476.26mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pe... [more]
KAG7023508.15.7e-14375.67Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosper... [more]
Match NameE-valueIdentityDescription
Q9SND63.3e-4537.87Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370... [more]
Q9CAD54.5e-3439.56Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3... [more]
O808883.8e-3331.85Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370... [more]
F4HRJ41.4e-3036.36Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702... [more]
Q405411.2e-2935.59Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097... [more]
Match NameE-valueIdentityDescription
A0A0A0LHV11.0e-15079.28Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G829... [more]
A0A5D3D3K94.0e-15079.28Mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo var. ma... [more]
A0A1S3B8294.0e-15079.28mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo OX=3656... [more]
A0A6J1HBP55.2e-14275.07mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=... [more]
A0A6J1JAH82.2e-10078.26mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita maxima OX=36... [more]
Match NameE-valueIdentityDescription
AT4G36950.11.8e-4642.92mitogen-activated protein kinase kinase kinase 21 [more]
AT3G50310.12.4e-4637.87mitogen-activated protein kinase kinase kinase 20 [more]
AT5G67080.12.2e-4438.87mitogen-activated protein kinase kinase kinase 19 [more]
AT5G55090.11.8e-3840.17mitogen-activated protein kinase kinase kinase 15 [more]
AT3G45670.16.9e-3832.41Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 68..330
e-value: 1.9E-41
score: 153.7
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 112..330
e-value: 4.3E-38
score: 131.2
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 43..330
score: 34.20924
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 83..332
e-value: 1.3E-48
score: 167.9
NoneNo IPR availablePANTHERPTHR48011:SF55SUBFAMILY NOT NAMEDcoord: 51..330
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 51..330
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 197..209
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 99..330

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10008061.1HG10008061.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity