HG10007864 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10007864 vs. NCBI nr
Match: XP_038880540.1 (rubisco accumulation factor 1.1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 805.4 bits (2079), Expect = 2.4e-229
Identity = 409/450 (90.89%), Postives = 429/450 (95.33%), Query Frame = 0

Query: 1   MLSTTTTANSLTSLSAAAHTTATFLHPPRGSPLHLFLRRRPKPISAIINPFPPPLAAKQQ 60
           MLSTTTTANSLTSLSAAAHTTATFLHPPR S L LFLRRRP  ISA +N  P PLAAKQQ
Sbjct: 1   MLSTTTTANSLTSLSAAAHTTATFLHPPRDSSLRLFLRRRPNSISATLN--PSPLAAKQQ 60

Query: 61  VYQPFRPPPSPLPSQYRSLDAEGKLNVLSNRLGLWFEYAPLISSLIQEGFTPPTLEEITG 120
           VYQPFRPPPSPLPSQYRSLDAEGKLNVLSNRLGLWFEYAPLISSLIQEGFTPPTLEEITG
Sbjct: 61  VYQPFRPPPSPLPSQYRSLDAEGKLNVLSNRLGLWFEYAPLISSLIQEGFTPPTLEEITG 120

Query: 121 ISGVQQNIFVVGAQVRESLVQSNNSDPDVIAAFDTSGAELLYELRLVSTEKRAAAAKYIV 180
           I+GVQQNIFVVGAQVRESLVQSNN+DPDVIAAFDTSGAELLYE+RL+STEKRAAAA+YIV
Sbjct: 121 ITGVQQNIFVVGAQVRESLVQSNNADPDVIAAFDTSGAELLYEIRLLSTEKRAAAARYIV 180

Query: 181 ENRLDSKGAQDLARAMKDFPRRRGDKGWECFDYDSPGDCLAFMYYRLSREYNNPSEPRTV 240
           ENRLD KGAQDLARA+KDFPRRRGDKGWECFDY+ PGDCLA+MYYRLSREYNNPSEPRT 
Sbjct: 181 ENRLDIKGAQDLARALKDFPRRRGDKGWECFDYEEPGDCLAYMYYRLSREYNNPSEPRTA 240

Query: 241 ALEDALKVVVTEKARDLIVGDLEGKGDGEEGDEEEIGAAVRVPVVRMKIGEVAEATTVVM 300
           ALE+ALKVVVTEKARDLIVGDLEGKGDG+EG EEEI   +RVPVVRMKIGEVAEATTV++
Sbjct: 241 ALEEALKVVVTEKARDLIVGDLEGKGDGKEGVEEEIEVGIRVPVVRMKIGEVAEATTVLV 300

Query: 301 MPVCKAGKGEKGVGEAPMEVRSEGEFGVVVVEKEWRRWVVLPGWEPVAGLMKGGGVVVAF 360
           MPVCKAG+GEKGVGEAPME+RSEGEFGVVV EKEWR+WVVLPGWEPV GL+KGGGVVVAF
Sbjct: 301 MPVCKAGEGEKGVGEAPMELRSEGEFGVVVAEKEWRKWVVLPGWEPVVGLVKGGGVVVAF 360

Query: 361 EDARALPWRVNRWYKEEPILVVADRNRKEVVAGDGFYLTGGTVGGGGELKVERGNALMEM 420
           EDARALPWRVNRWYKEEPILVV DR+RKEVVAGDGFYLTG TV GGGELKV+RGNALMEM
Sbjct: 361 EDARALPWRVNRWYKEEPILVVVDRSRKEVVAGDGFYLTGATV-GGGELKVQRGNALMEM 420

Query: 421 GVKESLGTVLLVVRPPKEMEDDQLSDEDWD 451
           GVKESLGTVLLVVRPPKEMEDDQLSDEDWD
Sbjct: 421 GVKESLGTVLLVVRPPKEMEDDQLSDEDWD 447

BLAST of HG10007864 vs. NCBI nr
Match: TYK28779.1 (rubisco accumulation factor 1 [Cucumis melo var. makuwa])

HSP 1 Score: 773.9 bits (1997), Expect = 7.8e-220
Identity = 395/451 (87.58%), Postives = 417/451 (92.46%), Query Frame = 0

Query: 3   STTTTANSLTSLSAAAHTTATFLHPPRGSPLHLFLRRRPKPISAIINPFPPPLAAKQQVY 62
           ST T ANSLTSLS+ AHTTA FLH P  SPL LFLRRR  PISAIINP P PLAAKQQVY
Sbjct: 4   STITNANSLTSLSSIAHTTAIFLHQPIDSPLRLFLRRRSNPISAIINPSPSPLAAKQQVY 63

Query: 63  QPFRPPPSPLPSQYRSLDAEGKLNVLSNRLGLWFEYAPLISSLIQEGFTPPTLEEITGIS 122
           QPFRPPPSPLP QYRSLD EGKLNVLSNRLGLWFEYAPLISSL+QEGFTPPTLEEITGIS
Sbjct: 64  QPFRPPPSPLPPQYRSLDTEGKLNVLSNRLGLWFEYAPLISSLLQEGFTPPTLEEITGIS 123

Query: 123 GVQQNIFVVGAQVRESLVQSNNSDPDVIAAFDTSGAELLYELRLVSTEKRAAAAKYIVEN 182
           GVQQN FVVGAQVRESLVQSN+SDPDVIA+FDTSGAELLYE+RL+STEKR AAAKYIVEN
Sbjct: 124 GVQQNTFVVGAQVRESLVQSNDSDPDVIASFDTSGAELLYEIRLLSTEKRVAAAKYIVEN 183

Query: 183 RLDSKGAQDLARAMKDFPRRRGDKGWECFDYDSPGDCLAFMYYRLSREYNNPSEPRTVAL 242
           RLDSKGAQDLARA+KDFPRR+GD+GWECFDYDS GDCLA+MYYRLSREYNN SE RT AL
Sbjct: 184 RLDSKGAQDLARAIKDFPRRKGDRGWECFDYDSAGDCLAYMYYRLSREYNNSSERRTAAL 243

Query: 243 EDALKVVVTEKARDLIVGDLEGKGDGEEGDEEEIGAAVRVPVVRMKIGEVAEATTVVMMP 302
           E+ALKVVVTEKAR+LIVGDLE KGDG++G EEE GA VRVPVVR+KIGEVAEAT VV+MP
Sbjct: 244 EEALKVVVTEKARNLIVGDLERKGDGKDGVEEETGAGVRVPVVRLKIGEVAEATKVVVMP 303

Query: 303 VCKAGKGEKGVGEAPMEVRSEGEFGVVVVEKEWRRWVVLPGWEPVAGLMKGGGVVVAFED 362
           VCKAG+GEKGVGEAPMEVRSEG+FGVVV EK W RWVVLPGWEPVAGL+KGGGVVVAF+D
Sbjct: 304 VCKAGEGEKGVGEAPMEVRSEGDFGVVVAEKGWSRWVVLPGWEPVAGLVKGGGVVVAFKD 363

Query: 363 ARALPWRVNRWYKEEPILVVADRNRKEVVAGDGFYLTGGTVG---GGGELKVERGNALME 422
           AR LPWRVNRWYKEEPILVVADR+RKEVVAGDGFYL GGTVG   GGGELKVERGNAL E
Sbjct: 364 ARVLPWRVNRWYKEEPILVVADRSRKEVVAGDGFYLMGGTVGGGDGGGELKVERGNALTE 423

Query: 423 MGVKESLGTVLLVVRPPKEMEDDQLSDEDWD 451
           MGVKESLGTV+LVVRPPKEMEDDQLSDEDWD
Sbjct: 424 MGVKESLGTVVLVVRPPKEMEDDQLSDEDWD 454

BLAST of HG10007864 vs. NCBI nr
Match: XP_008462710.1 (PREDICTED: rubisco accumulation factor 1, chloroplastic [Cucumis melo])

HSP 1 Score: 772.7 bits (1994), Expect = 1.7e-219
Identity = 394/451 (87.36%), Postives = 417/451 (92.46%), Query Frame = 0

Query: 3   STTTTANSLTSLSAAAHTTATFLHPPRGSPLHLFLRRRPKPISAIINPFPPPLAAKQQVY 62
           ST T ANSLTSLS+ AHTTA FLH P  SPL LFLRRR  PISAIINP P PLAAKQQVY
Sbjct: 4   STITNANSLTSLSSIAHTTAIFLHQPIDSPLRLFLRRRSNPISAIINPSPSPLAAKQQVY 63

Query: 63  QPFRPPPSPLPSQYRSLDAEGKLNVLSNRLGLWFEYAPLISSLIQEGFTPPTLEEITGIS 122
           QPFRPPPSPLP QYRSLD EGKLNVLSNRLGLWF+YAPLISSL+QEGFTPPTLEEITGIS
Sbjct: 64  QPFRPPPSPLPPQYRSLDTEGKLNVLSNRLGLWFQYAPLISSLLQEGFTPPTLEEITGIS 123

Query: 123 GVQQNIFVVGAQVRESLVQSNNSDPDVIAAFDTSGAELLYELRLVSTEKRAAAAKYIVEN 182
           GVQQN FVVGAQVRESLVQSN+SDPDVIA+FDTSGAELLYE+RL+STEKR AAAKYIVEN
Sbjct: 124 GVQQNTFVVGAQVRESLVQSNDSDPDVIASFDTSGAELLYEIRLLSTEKRVAAAKYIVEN 183

Query: 183 RLDSKGAQDLARAMKDFPRRRGDKGWECFDYDSPGDCLAFMYYRLSREYNNPSEPRTVAL 242
           RLDSKGAQDLARA+KDFPRR+GD+GWECFDYDS GDCLA+MYYRLSREYNN SE RT AL
Sbjct: 184 RLDSKGAQDLARAIKDFPRRKGDRGWECFDYDSAGDCLAYMYYRLSREYNNSSERRTAAL 243

Query: 243 EDALKVVVTEKARDLIVGDLEGKGDGEEGDEEEIGAAVRVPVVRMKIGEVAEATTVVMMP 302
           E+ALKVVVTEKAR+LIVGDLE KGDG++G EEE GA VRVPVVR+KIGEVAEAT VV+MP
Sbjct: 244 EEALKVVVTEKARNLIVGDLERKGDGKDGVEEETGAGVRVPVVRLKIGEVAEATKVVVMP 303

Query: 303 VCKAGKGEKGVGEAPMEVRSEGEFGVVVVEKEWRRWVVLPGWEPVAGLMKGGGVVVAFED 362
           VCKAG+GEKGVGEAPMEVRSEG+FGVVV EK W RWVVLPGWEPVAGL+KGGGVVVAF+D
Sbjct: 304 VCKAGEGEKGVGEAPMEVRSEGDFGVVVAEKGWSRWVVLPGWEPVAGLVKGGGVVVAFKD 363

Query: 363 ARALPWRVNRWYKEEPILVVADRNRKEVVAGDGFYLTGGTVG---GGGELKVERGNALME 422
           AR LPWRVNRWYKEEPILVVADR+RKEVVAGDGFYL GGTVG   GGGELKVERGNAL E
Sbjct: 364 ARVLPWRVNRWYKEEPILVVADRSRKEVVAGDGFYLMGGTVGGGDGGGELKVERGNALTE 423

Query: 423 MGVKESLGTVLLVVRPPKEMEDDQLSDEDWD 451
           MGVKESLGTV+LVVRPPKEMEDDQLSDEDWD
Sbjct: 424 MGVKESLGTVVLVVRPPKEMEDDQLSDEDWD 454

BLAST of HG10007864 vs. NCBI nr
Match: KAA0062559.1 (rubisco accumulation factor 1 [Cucumis melo var. makuwa])

HSP 1 Score: 771.2 bits (1990), Expect = 5.1e-219
Identity = 394/451 (87.36%), Postives = 416/451 (92.24%), Query Frame = 0

Query: 3   STTTTANSLTSLSAAAHTTATFLHPPRGSPLHLFLRRRPKPISAIINPFPPPLAAKQQVY 62
           ST T ANSLTSLS+ AHTTA FLH P  SPL LFLRRR  PISAIINP P PLAAKQQVY
Sbjct: 4   STITNANSLTSLSSIAHTTAIFLHQPIDSPLRLFLRRRSNPISAIINPSPSPLAAKQQVY 63

Query: 63  QPFRPPPSPLPSQYRSLDAEGKLNVLSNRLGLWFEYAPLISSLIQEGFTPPTLEEITGIS 122
           QPFRPPPSPLP QYRSLD EGKLNVLSNRLGLWFEYAPLISSL+QEGFTPPTLEEITGIS
Sbjct: 64  QPFRPPPSPLPPQYRSLDTEGKLNVLSNRLGLWFEYAPLISSLLQEGFTPPTLEEITGIS 123

Query: 123 GVQQNIFVVGAQVRESLVQSNNSDPDVIAAFDTSGAELLYELRLVSTEKRAAAAKYIVEN 182
           GVQQN FVVGAQVRESLVQSN+SDPDVIA+FDTSGAELLYE+RL+STEKR AAAKYIVEN
Sbjct: 124 GVQQNTFVVGAQVRESLVQSNDSDPDVIASFDTSGAELLYEIRLLSTEKRVAAAKYIVEN 183

Query: 183 RLDSKGAQDLARAMKDFPRRRGDKGWECFDYDSPGDCLAFMYYRLSREYNNPSEPRTVAL 242
           RLDSKGAQDLARA+KDFPRR+GD+GWECFDYDS GDCLA+MYYRLSREYNN SE RT AL
Sbjct: 184 RLDSKGAQDLARAIKDFPRRKGDRGWECFDYDSAGDCLAYMYYRLSREYNNSSERRTAAL 243

Query: 243 EDALKVVVTEKARDLIVGDLEGKGDGEEGDEEEIGAAVRVPVVRMKIGEVAEATTVVMMP 302
           E+ALKV VTEKAR+LIVGDLE KGDG++G EEE GA VRVPVVR+KIGEVAEAT VV+MP
Sbjct: 244 EEALKVGVTEKARNLIVGDLERKGDGKDGVEEETGAGVRVPVVRLKIGEVAEATKVVVMP 303

Query: 303 VCKAGKGEKGVGEAPMEVRSEGEFGVVVVEKEWRRWVVLPGWEPVAGLMKGGGVVVAFED 362
           VCKAG+GEKGVGEAPMEVRSEG+FGVVV EK W RWVVLPGWEPVAGL+KGGGVVVAF+D
Sbjct: 304 VCKAGEGEKGVGEAPMEVRSEGDFGVVVAEKGWSRWVVLPGWEPVAGLVKGGGVVVAFKD 363

Query: 363 ARALPWRVNRWYKEEPILVVADRNRKEVVAGDGFYLTGGTVG---GGGELKVERGNALME 422
           AR LPWRVNRWYKEEPILVVADR+RKEVVAGDGFYL GGTVG   GGGELKVERGNAL E
Sbjct: 364 ARVLPWRVNRWYKEEPILVVADRSRKEVVAGDGFYLMGGTVGGGDGGGELKVERGNALTE 423

Query: 423 MGVKESLGTVLLVVRPPKEMEDDQLSDEDWD 451
           MGVKESLGTV+LVVRPPKEMEDDQLSDEDWD
Sbjct: 424 MGVKESLGTVVLVVRPPKEMEDDQLSDEDWD 454

BLAST of HG10007864 vs. NCBI nr
Match: XP_004142574.1 (rubisco accumulation factor 1.1, chloroplastic [Cucumis sativus] >KGN66790.1 hypothetical protein Csa_007640 [Cucumis sativus])

HSP 1 Score: 762.3 bits (1967), Expect = 2.3e-216
Identity = 389/448 (86.83%), Postives = 414/448 (92.41%), Query Frame = 0

Query: 3   STTTTANSLTSLSAAAHTTATFLHPPRGSPLHLFLRRRPKPISAIINPFPPPLAAKQQVY 62
           ST T ANSL+SLS+ AHTT+ FLH PR SPL LFLRRR  PISAIINP    LAAKQQVY
Sbjct: 4   STITNANSLSSLSSIAHTTSIFLHQPRDSPLRLFLRRRLNPISAIINPSSSSLAAKQQVY 63

Query: 63  QPFRPPPSPLPSQYRSLDAEGKLNVLSNRLGLWFEYAPLISSLIQEGFTPPTLEEITGIS 122
           QPFRPPPSPLP QYRSLD EGKLN+LSNRLGLWFEYAPLISSL+QEGFTPP LEEITGIS
Sbjct: 64  QPFRPPPSPLPPQYRSLDTEGKLNILSNRLGLWFEYAPLISSLLQEGFTPPVLEEITGIS 123

Query: 123 GVQQNIFVVGAQVRESLVQSNNSDPDVIAAFDTSGAELLYELRLVSTEKRAAAAKYIVEN 182
           GVQQN F+VGAQVRESL+QSN+SDPDVIA+FDT GAELLYE+RL+STEKRAAAAKYIVEN
Sbjct: 124 GVQQNSFIVGAQVRESLLQSNDSDPDVIASFDTGGAELLYEIRLLSTEKRAAAAKYIVEN 183

Query: 183 RLDSKGAQDLARAMKDFPRRRGDKGWECFDYDSPGDCLAFMYYRLSREYNNPSEPRTVAL 242
           RLDSKGAQDLARAMKDFPRRRGDKGWE FDYD  GDCLA+MYYRLSREYN+ +E RT AL
Sbjct: 184 RLDSKGAQDLARAMKDFPRRRGDKGWEYFDYDFAGDCLAYMYYRLSREYNSSTERRTAAL 243

Query: 243 EDALKVVVTEKARDLIVGDLEGKGDGEEGDEEEIGAAVRVPVVRMKIGEVAEATTVVMMP 302
           E+ALKVVVTEKARDLIVGDLEGKGDG++G EEEIGAAV+VPVVRMKIGEVAEATTVV+MP
Sbjct: 244 EEALKVVVTEKARDLIVGDLEGKGDGKDGVEEEIGAAVKVPVVRMKIGEVAEATTVVVMP 303

Query: 303 VCKAGKGEKGVGEAPMEVRSEGEFGVVVVEKEWRRWVVLPGWEPVAGLMKGGGVVVAFED 362
           VCKAG+GEKGVGEAPMEVRSEGEFGVVV EK W RWVVLPGWEPVAGL+KGGGVVVAFED
Sbjct: 304 VCKAGEGEKGVGEAPMEVRSEGEFGVVVAEKGWSRWVVLPGWEPVAGLVKGGGVVVAFED 363

Query: 363 ARALPWRVNRWYKEEPILVVADRNRKEVVAGDGFYLTGGTVGGGGELKVERGNALMEMGV 422
           AR LPWRVNRWYKEEPILVVADR+R+EVVAGDGFYL GG   GGG+LKVERGNALMEMGV
Sbjct: 364 ARVLPWRVNRWYKEEPILVVADRSRREVVAGDGFYLMGGG-DGGGDLKVERGNALMEMGV 423

Query: 423 KESLGTVLLVVRPPKEMEDDQLSDEDWD 451
           KESLGTVLLVVRPP+E EDDQLSDEDWD
Sbjct: 424 KESLGTVLLVVRPPRETEDDQLSDEDWD 450

BLAST of HG10007864 vs. ExPASy Swiss-Prot
Match: Q9LKR8 (Rubisco accumulation factor 1.1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RAF1.1 PE=1 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 2.9e-124
Identity = 249/449 (55.46%), Postives = 321/449 (71.49%), Query Frame = 0

Query: 11  LTSLSAAAHTTATFLHPPRGSPLHLFLRRRP---KPISAIINPFPP----PLAAKQQVYQ 70
           + SL+A   +++ F      S  H F   RP   KP + I +   P        KQQ+YQ
Sbjct: 1   MLSLTATTLSSSIFTQ----SKTHGFFNTRPVYRKPFTTITSALIPASNRQAPPKQQLYQ 60

Query: 71  PFRPPPSPLPSQYRSLDAEGKLNVLSNRLGLWFEYAPLISSLIQEGFTPPTLEEITGISG 130
           PFRPPPSP+P ++RSLD  GK+ VL++RLGLWFEYAPLISSL  EGFTPP++EE+TGISG
Sbjct: 61  PFRPPPSPIPPKFRSLDTAGKIEVLADRLGLWFEYAPLISSLYTEGFTPPSIEELTGISG 120

Query: 131 VQQNIFVVGAQVRESLVQSNNSDPDVIAAFDTSGAELLYELRLVSTEKRAAAAKYIVENR 190
           V+QN  +VGAQVR+SLVQS  + P++IAAFDT+GAELLYE+RL++T +R AAA+YIV++ 
Sbjct: 121 VEQNSLIVGAQVRDSLVQS-GAKPELIAAFDTNGAELLYEIRLLNTTQRVAAAEYIVDHG 180

Query: 191 LDSKGAQDLARAMKDFPRRRGDKGWECFDYDSPGDCLAFMYYRLSREYNNPSEPRTVALE 250
            D+KGA DLARA+KDFP RRGD G   FDY+ PGDCL+FM YR SRE+ +PSE RT  LE
Sbjct: 181 FDTKGAGDLARAIKDFPHRRGDVGLGDFDYNLPGDCLSFMLYRKSREHRSPSEIRTTLLE 240

Query: 251 DALKVVVTEKARDLIVGDLEGKGDGEEGDEEEIGAAVRVPVVRMKIGEVAEATTVVMMPV 310
            AL+  VTEKA+  ++ +L G+ + E   EEEI   +RVPVVR++ GEVA A++VV++PV
Sbjct: 241 QALETAVTEKAKKAVLRELHGESEEERVKEEEI-KIIRVPVVRLRFGEVAGASSVVVLPV 300

Query: 311 CKAGKGEKGVGEAPMEVRSEGEFGVVVVEKEWRRWVVLPGWEPVAGLMKGGGVVVAFEDA 370
           CKA +GE+ + EAPME  S GEFGVV  EK+W RWVVLPGW+PV  + K  GV V+F D 
Sbjct: 301 CKAEEGEEKLLEAPMEFESGGEFGVVEAEKDWSRWVVLPGWDPVVAVRK--GVAVSFSDD 360

Query: 371 R-ALPWRVNRWYKEEPILVVADRNRKEVVAGDGFYLTGGTVGGGGELKVERGNALMEMGV 430
           R  LPW      K E I+VV DR +K V A +G+Y     V   G +K++RG  L E GV
Sbjct: 361 REVLPWN----GKGEAIMVVIDREKKTVEADNGYYY---LVVADGGMKLDRGLVLKEKGV 420

Query: 431 KESLGTVLLVVRPPKEMEDD-QLSDEDWD 451
            ESLG V+LVVRPP++ +D+ Q++DEDWD
Sbjct: 421 NESLGMVVLVVRPPRDDDDEWQINDEDWD 434

BLAST of HG10007864 vs. ExPASy Swiss-Prot
Match: Q9SR19 (Rubisco accumulation factor 1.2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RAF1.2 PE=1 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 3.2e-123
Identity = 236/415 (56.87%), Postives = 307/415 (73.98%), Query Frame = 0

Query: 41  PKPISAIINPFPPPLAAKQQVYQPFRPPPSPLPSQYRSLDAEGKLNVLSNRLGLWFEYAP 100
           PK  SA + P  PP  A+QQ+YQPFRPP SP+P+Q+RSLD+ GK+ +L+ R+ LWFEYAP
Sbjct: 46  PKRSSANMIPKNPP--ARQQLYQPFRPPSSPIPTQFRSLDSAGKIEILAGRMALWFEYAP 105

Query: 101 LISSLIQEGFTPPTLEEITGISGVQQNIFVVGAQVRESLVQSNNSDPDVIAAFDTSGAEL 160
           LISSL  +GFTPPT+EE+TGIS ++QN  +VGAQVR+S++QS + +P++I+AFDT GAEL
Sbjct: 106 LISSLYTDGFTPPTIEELTGISSIEQNRLIVGAQVRDSILQSIH-EPELISAFDTGGAEL 165

Query: 161 LYELRLVSTEKRAAAAKYIVENRLDSKGAQDLARAMKDFPRRRGDKGWECFDYDSPGDCL 220
           LYE+RL+ST +R AAA +I++  +DSKGAQDLARA+KD+P RRGD GW  FDY+ PGDCL
Sbjct: 166 LYEIRLLSTTQRVAAATFIIDRNIDSKGAQDLARAIKDYPNRRGDVGWLDFDYNLPGDCL 225

Query: 221 AFMYYRLSREYNNPSEPRTVALEDALKVVVTEKARDL----IVGDLEGKGDGEEGDEEEI 280
           +F+YYR SRE  NPS+ RT  L  AL V  +EKA++     + GD E + + E+  +EE 
Sbjct: 226 SFLYYRQSRENKNPSDQRTSMLLQALGVAESEKAKNRLNTELYGDKEAEKEKEKKKKEEE 285

Query: 281 GAAVRVPVVRMKIGEVAEATTVVMMPVCKAGKGEKGVGEAPMEVRSEGEFGVVVVEKEWR 340
             A+R+PVVR+K GEVAEAT+VV++PVCKA +GEK + EAPME+ + G+F VV  EK W+
Sbjct: 286 VKAIRIPVVRLKFGEVAEATSVVVLPVCKAEEGEKKILEAPMEIIAGGDFKVVEAEKGWK 345

Query: 341 RWVVLPGWEPVAGLMKGGGVVVAFEDARALPWRVNRWYKEEPILVVADRNRKEVVAGDGF 400
           RWVVLP W PVA + KGG  V   +D + LPW      KEEP+LVVADR R  V A DG+
Sbjct: 346 RWVVLPSWNPVAAIGKGGVAVSFRDDRKVLPWD----GKEEPLLVVADRVRNVVEADDGY 405

Query: 401 YLTGGTVGGGGELKVERGNALMEMGVKESLGTVLLVVRPPKEMEDD-QLSDEDWD 451
           YL     G    LK+E+G+ L    VKESLG V+LVVRPP+E +DD Q S ++WD
Sbjct: 406 YLVVAENG----LKLEKGSDLKAREVKESLGMVVLVVRPPREDDDDWQTSHQNWD 449

BLAST of HG10007864 vs. ExPASy Swiss-Prot
Match: Q7XDY9 (Rubisco accumulation factor 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RAF1 PE=2 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 3.0e-97
Identity = 213/443 (48.08%), Postives = 283/443 (63.88%), Query Frame = 0

Query: 20  TTATFLHPPRGSPLHLFLRRRPKPISAIINPFPPPLAAKQQVYQPFRPPPSPLPSQYRSL 79
           TT+  +  P G P+    R    P +      PPP A   Q+YQPF PPPSPLP +Y+ L
Sbjct: 44  TTSAVILGPDGRPIGGGPRDNKLPFT------PPPTAPPDQLYQPFHPPPSPLPDKYKDL 103

Query: 80  DAEGKLNVLSNRLGLWFEYAPLISSLIQEGFTPPTLEEITGISGVQQNIFVVGAQVRESL 139
           D   +L VL +RLGLW EYAPLIS+L +EGFTP ++EE TGISGV+QN  VV  QVR+SL
Sbjct: 104 DLGQRLAVLRDRLGLWHEYAPLISALSREGFTPSSIEEATGISGVEQNSVVVATQVRDSL 163

Query: 140 VQSNNSDP-DVIAAFDT-SGAELLYELRLVSTEKRAAAAKYIVENRLDSKGAQDLARAMK 199
           V      P +++  FD+  G ELLYELR ++  +RA AA++ ++ RL+ +G ++LAR+MK
Sbjct: 164 VADEGGFPAELLRYFDSYGGPELLYELRFLNARQRADAARHAIDRRLEPRGVRELARSMK 223

Query: 200 DFPRRRGDKGWECFDYDSPGDCLAFMYYRLSREYNNPSEPRTVALEDALKVVVTEKARDL 259
           DFP+RRGD GWE F  D+PGDCLAF  +R SRE  + +E     LE AL+VV TE AR  
Sbjct: 224 DFPQRRGDDGWEAFTRDNPGDCLAFARFRQSREAID-AEDSVAELERALEVVDTEPARAR 283

Query: 260 IVGDLE---GKGDGEEGDEEE-----IGAAVR--VPVVRMKIGEVAEATTVVMMPVCKAG 319
           +  +L+    K  GEE D+E+       AA R  VPVVR+  GEVAEATTV+++PV + G
Sbjct: 284 VEAELDRARRKAAGEEVDDEDGAANAAAAASRPAVPVVRLMYGEVAEATTVLLLPVVREG 343

Query: 320 KGEKGVGEAPMEVRSEGEFGVVVVEKEWRRWVVLPGWEPVAGLMKGGGVVVAFEDARALP 379
            G + +  AP   R++ + G+V V+K W RW V+PGW PVA  + G  VV+   D R LP
Sbjct: 344 DGGEALAHAPRRTRTDADLGMVEVDKGWTRWAVVPGWGPVAE-VAGEAVVIELADGRTLP 403

Query: 380 WRVNRWYKEEPILVVADRNRKEVVAGDGFYLTGGTVGGGGELKVERGNALMEMGVKESLG 439
           WR     + E +LVVA+R R+EVV  DG Y+    V   G L VE+G  L   GV E+  
Sbjct: 404 WRS---AEAERVLVVANRGRREVVE-DGIYV----VEREGRLVVEKGRKLAAEGVGEAAA 463

Query: 440 TVLLVVRPPKEMEDDQLSDEDWD 451
            VL+VVRPP++ EDD +SD++WD
Sbjct: 464 EVLVVVRPPRD-EDDMISDDEWD 469

BLAST of HG10007864 vs. ExPASy Swiss-Prot
Match: B4FR29 (Rubisco accumulation factor 1, chloroplastic OS=Zea mays OX=4577 GN=RAF1 PE=1 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 4.5e-93
Identity = 199/415 (47.95%), Postives = 272/415 (65.54%), Query Frame = 0

Query: 50  PF-PPPLAAKQQVYQPFRPPPSPLPSQYRSLDAEGKLNVLSNRLGLWFEYAPLISSLIQE 109
           PF PPP+A   Q+YQPF PPPSPLP   R+LD   +L +L +R+GLW EYAPLISSL ++
Sbjct: 60  PFTPPPMAPPGQLYQPFHPPPSPLPPSLRNLDLSERLQILRDRMGLWHEYAPLISSLSRD 119

Query: 110 GFTPPTLEEITGISGVQQNIFVVGAQVRESLVQSNNS--DPDVIAAFDT-SGAELLYELR 169
           GF P +++E TGISGV+QN  VV +QVR+SL+    +   PD++  FD+  G E+LYELR
Sbjct: 120 GFNPSSIQEATGISGVEQNCLVVASQVRDSLLDDRAAAFPPDLLPYFDSLGGPEVLYELR 179

Query: 170 LVSTEKRAAAAKYIVENRLDSKGAQDLARAMKDFPRRRGDKGWECFDYDSPGDCLAFMYY 229
            ++  +RA AA++ +  RL+ KG ++LARAMK FPR RG++GWE F  DSP DCLAF  +
Sbjct: 180 FLNARQRADAARHAIGYRLEPKGVRELARAMKGFPRWRGEEGWEAFSKDSPADCLAFARF 239

Query: 230 RLSREYNNPSEPRTVALEDALKVVVTEKARDLIVGDLE---GKGDG-----EEGDEEEIG 289
           R SRE  +  + R   LE AL+VV TE  R  +  +LE    K  G     EEG+E++  
Sbjct: 240 RQSREAIDVQD-RVAELERALQVVETESGRARVELELERARRKAAGEEEVDEEGEEDDAA 299

Query: 290 AAVR--VPVVRMKIGEVAEATTVVMMPVCKAGKGEKGVGEAPMEVRSEGEFGVVVVEKEW 349
           A++R  V VVR++ GEVAEATTV+++PV +   G   +  AP   +++   GVV V++ W
Sbjct: 300 ASLRPGVTVVRLRYGEVAEATTVILLPVVRETDGVAAMESAPRRAKTDVGLGVVEVDRAW 359

Query: 350 RRWVVLPGWEPVAGLMKGGGVVVAFEDARALPWRVNRWYKEEPILVVADRNRKEVVAGDG 409
            RW V+PGW PVA       VVV   D R LPWR++    EEP+LV+A+R++KEVV  +G
Sbjct: 360 ARWAVVPGWGPVAE-AADDAVVVELADGRRLPWRMS---DEEPVLVIANRSKKEVVE-EG 419

Query: 410 FYLTGGTVGGGGELKVERGNALMEMGVKESLGTVLLVVRPPKEMEDDQLSDEDWD 451
            Y+    +   G L VERG  L E G+  +   V++VVRPPK+ EDD +SDE+WD
Sbjct: 420 VYV----LEREGRLVVERGKKLAEQGIAAAAAEVVIVVRPPKD-EDDMVSDEEWD 463

BLAST of HG10007864 vs. ExPASy Swiss-Prot
Match: Q8YLP6 (RuBisCO accumulation factor 1 OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=raf1 PE=1 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 9.8e-24
Identity = 110/389 (28.28%), Postives = 190/389 (48.84%), Query Frame = 0

Query: 67  PPPSPLPSQYRSLDAEGKLNVLSNRLGLWFEYAPLISSLIQEGFTPPTLEEITGISGVQQ 126
           PP +P P    +  A+  L  L  + G W E+   I+SL + G+ P  + E TG   VQQ
Sbjct: 5   PPNAPNPENATNELAQELLRKLRQKQGNWVEWGQAIASLQKSGYNPQDIFEATGFEPVQQ 64

Query: 127 NIFVVGAQVRESLVQSNNSDPDVIAAFDTSGAELLYELRLVSTEKRAAAAKYIVENRLDS 186
           N  +VG+QV  SL +S  S    +A + T G+++LYELRL++ E+RAAA      +++D+
Sbjct: 65  NQVIVGSQVYNSLEKSGAS-AATLAHYATRGSDVLYELRLLTHEERAAAGDLTFTHKVDA 124

Query: 187 KGAQDLARAMKDFPR-RRGDKGWECFDYDSPGDCLAFMYYRLSREYNNPSEPRTVALEDA 246
             A+++A+A+KDF R R   +G+     + PGD +A+  ++L+R+Y++  E R+  +   
Sbjct: 125 DEAREIAKAIKDFSRFRILPEGFS----NHPGDAVAYQAWKLARQYSDLQE-RSRLIARG 184

Query: 247 LKVVVTEKARDLIVGDLEGKGDGEEGDEEEIGAAVRVPVVRMKIGEVAEATTVVMMPVCK 306
           L+   +E AR  I   L          +  + +    P+      +  +    ++  V +
Sbjct: 185 LRFAHSETARKQIEQLLV---------DFTVVSQRPAPIPPFFRFDTEDELPRIVPVVGQ 244

Query: 307 AGKGEKGVGEAPMEVRSEGEFGVVVVEKEWRRWVVLPGWEPVAGLMKGGGVVVAFEDARA 366
                + +   P+ V     F +V    E + WV LPGW+          V++A ED   
Sbjct: 245 LPLKAEELKAVPL-VEEIEPFRLVKFSGE-QAWVALPGWQ----------VLLAAEDPVT 304

Query: 367 LPWRVNRWYKEE-----PILVVADRNRKEVVAGDGFYLTGGTVGGGGELKVERGNALMEM 426
           +    +R+ K+      P+LVV DR+++E    D  Y     V   GEL  +      E 
Sbjct: 305 ILATSDRFPKQNQTEPGPVLVVVDRSQRE--WNDFSYF---VVDHDGELDFQWFETKPEF 357

Query: 427 GVKESLGTVLLVVRPPKEMEDDQLSDEDW 450
            +   LG V+++VR P+ + D+ ++ + W
Sbjct: 365 PI---LGKVIILVR-PRRILDENVTKDSW 357

BLAST of HG10007864 vs. ExPASy TrEMBL
Match: A0A5D3DZE4 (Rubisco accumulation factor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G001420 PE=4 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 3.8e-220
Identity = 395/451 (87.58%), Postives = 417/451 (92.46%), Query Frame = 0

Query: 3   STTTTANSLTSLSAAAHTTATFLHPPRGSPLHLFLRRRPKPISAIINPFPPPLAAKQQVY 62
           ST T ANSLTSLS+ AHTTA FLH P  SPL LFLRRR  PISAIINP P PLAAKQQVY
Sbjct: 4   STITNANSLTSLSSIAHTTAIFLHQPIDSPLRLFLRRRSNPISAIINPSPSPLAAKQQVY 63

Query: 63  QPFRPPPSPLPSQYRSLDAEGKLNVLSNRLGLWFEYAPLISSLIQEGFTPPTLEEITGIS 122
           QPFRPPPSPLP QYRSLD EGKLNVLSNRLGLWFEYAPLISSL+QEGFTPPTLEEITGIS
Sbjct: 64  QPFRPPPSPLPPQYRSLDTEGKLNVLSNRLGLWFEYAPLISSLLQEGFTPPTLEEITGIS 123

Query: 123 GVQQNIFVVGAQVRESLVQSNNSDPDVIAAFDTSGAELLYELRLVSTEKRAAAAKYIVEN 182
           GVQQN FVVGAQVRESLVQSN+SDPDVIA+FDTSGAELLYE+RL+STEKR AAAKYIVEN
Sbjct: 124 GVQQNTFVVGAQVRESLVQSNDSDPDVIASFDTSGAELLYEIRLLSTEKRVAAAKYIVEN 183

Query: 183 RLDSKGAQDLARAMKDFPRRRGDKGWECFDYDSPGDCLAFMYYRLSREYNNPSEPRTVAL 242
           RLDSKGAQDLARA+KDFPRR+GD+GWECFDYDS GDCLA+MYYRLSREYNN SE RT AL
Sbjct: 184 RLDSKGAQDLARAIKDFPRRKGDRGWECFDYDSAGDCLAYMYYRLSREYNNSSERRTAAL 243

Query: 243 EDALKVVVTEKARDLIVGDLEGKGDGEEGDEEEIGAAVRVPVVRMKIGEVAEATTVVMMP 302
           E+ALKVVVTEKAR+LIVGDLE KGDG++G EEE GA VRVPVVR+KIGEVAEAT VV+MP
Sbjct: 244 EEALKVVVTEKARNLIVGDLERKGDGKDGVEEETGAGVRVPVVRLKIGEVAEATKVVVMP 303

Query: 303 VCKAGKGEKGVGEAPMEVRSEGEFGVVVVEKEWRRWVVLPGWEPVAGLMKGGGVVVAFED 362
           VCKAG+GEKGVGEAPMEVRSEG+FGVVV EK W RWVVLPGWEPVAGL+KGGGVVVAF+D
Sbjct: 304 VCKAGEGEKGVGEAPMEVRSEGDFGVVVAEKGWSRWVVLPGWEPVAGLVKGGGVVVAFKD 363

Query: 363 ARALPWRVNRWYKEEPILVVADRNRKEVVAGDGFYLTGGTVG---GGGELKVERGNALME 422
           AR LPWRVNRWYKEEPILVVADR+RKEVVAGDGFYL GGTVG   GGGELKVERGNAL E
Sbjct: 364 ARVLPWRVNRWYKEEPILVVADRSRKEVVAGDGFYLMGGTVGGGDGGGELKVERGNALTE 423

Query: 423 MGVKESLGTVLLVVRPPKEMEDDQLSDEDWD 451
           MGVKESLGTV+LVVRPPKEMEDDQLSDEDWD
Sbjct: 424 MGVKESLGTVVLVVRPPKEMEDDQLSDEDWD 454

BLAST of HG10007864 vs. ExPASy TrEMBL
Match: A0A1S3CHL8 (rubisco accumulation factor 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501013 PE=4 SV=1)

HSP 1 Score: 772.7 bits (1994), Expect = 8.4e-220
Identity = 394/451 (87.36%), Postives = 417/451 (92.46%), Query Frame = 0

Query: 3   STTTTANSLTSLSAAAHTTATFLHPPRGSPLHLFLRRRPKPISAIINPFPPPLAAKQQVY 62
           ST T ANSLTSLS+ AHTTA FLH P  SPL LFLRRR  PISAIINP P PLAAKQQVY
Sbjct: 4   STITNANSLTSLSSIAHTTAIFLHQPIDSPLRLFLRRRSNPISAIINPSPSPLAAKQQVY 63

Query: 63  QPFRPPPSPLPSQYRSLDAEGKLNVLSNRLGLWFEYAPLISSLIQEGFTPPTLEEITGIS 122
           QPFRPPPSPLP QYRSLD EGKLNVLSNRLGLWF+YAPLISSL+QEGFTPPTLEEITGIS
Sbjct: 64  QPFRPPPSPLPPQYRSLDTEGKLNVLSNRLGLWFQYAPLISSLLQEGFTPPTLEEITGIS 123

Query: 123 GVQQNIFVVGAQVRESLVQSNNSDPDVIAAFDTSGAELLYELRLVSTEKRAAAAKYIVEN 182
           GVQQN FVVGAQVRESLVQSN+SDPDVIA+FDTSGAELLYE+RL+STEKR AAAKYIVEN
Sbjct: 124 GVQQNTFVVGAQVRESLVQSNDSDPDVIASFDTSGAELLYEIRLLSTEKRVAAAKYIVEN 183

Query: 183 RLDSKGAQDLARAMKDFPRRRGDKGWECFDYDSPGDCLAFMYYRLSREYNNPSEPRTVAL 242
           RLDSKGAQDLARA+KDFPRR+GD+GWECFDYDS GDCLA+MYYRLSREYNN SE RT AL
Sbjct: 184 RLDSKGAQDLARAIKDFPRRKGDRGWECFDYDSAGDCLAYMYYRLSREYNNSSERRTAAL 243

Query: 243 EDALKVVVTEKARDLIVGDLEGKGDGEEGDEEEIGAAVRVPVVRMKIGEVAEATTVVMMP 302
           E+ALKVVVTEKAR+LIVGDLE KGDG++G EEE GA VRVPVVR+KIGEVAEAT VV+MP
Sbjct: 244 EEALKVVVTEKARNLIVGDLERKGDGKDGVEEETGAGVRVPVVRLKIGEVAEATKVVVMP 303

Query: 303 VCKAGKGEKGVGEAPMEVRSEGEFGVVVVEKEWRRWVVLPGWEPVAGLMKGGGVVVAFED 362
           VCKAG+GEKGVGEAPMEVRSEG+FGVVV EK W RWVVLPGWEPVAGL+KGGGVVVAF+D
Sbjct: 304 VCKAGEGEKGVGEAPMEVRSEGDFGVVVAEKGWSRWVVLPGWEPVAGLVKGGGVVVAFKD 363

Query: 363 ARALPWRVNRWYKEEPILVVADRNRKEVVAGDGFYLTGGTVG---GGGELKVERGNALME 422
           AR LPWRVNRWYKEEPILVVADR+RKEVVAGDGFYL GGTVG   GGGELKVERGNAL E
Sbjct: 364 ARVLPWRVNRWYKEEPILVVADRSRKEVVAGDGFYLMGGTVGGGDGGGELKVERGNALTE 423

Query: 423 MGVKESLGTVLLVVRPPKEMEDDQLSDEDWD 451
           MGVKESLGTV+LVVRPPKEMEDDQLSDEDWD
Sbjct: 424 MGVKESLGTVVLVVRPPKEMEDDQLSDEDWD 454

BLAST of HG10007864 vs. ExPASy TrEMBL
Match: A0A5A7VA63 (Rubisco accumulation factor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold79G00440 PE=4 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 2.4e-219
Identity = 394/451 (87.36%), Postives = 416/451 (92.24%), Query Frame = 0

Query: 3   STTTTANSLTSLSAAAHTTATFLHPPRGSPLHLFLRRRPKPISAIINPFPPPLAAKQQVY 62
           ST T ANSLTSLS+ AHTTA FLH P  SPL LFLRRR  PISAIINP P PLAAKQQVY
Sbjct: 4   STITNANSLTSLSSIAHTTAIFLHQPIDSPLRLFLRRRSNPISAIINPSPSPLAAKQQVY 63

Query: 63  QPFRPPPSPLPSQYRSLDAEGKLNVLSNRLGLWFEYAPLISSLIQEGFTPPTLEEITGIS 122
           QPFRPPPSPLP QYRSLD EGKLNVLSNRLGLWFEYAPLISSL+QEGFTPPTLEEITGIS
Sbjct: 64  QPFRPPPSPLPPQYRSLDTEGKLNVLSNRLGLWFEYAPLISSLLQEGFTPPTLEEITGIS 123

Query: 123 GVQQNIFVVGAQVRESLVQSNNSDPDVIAAFDTSGAELLYELRLVSTEKRAAAAKYIVEN 182
           GVQQN FVVGAQVRESLVQSN+SDPDVIA+FDTSGAELLYE+RL+STEKR AAAKYIVEN
Sbjct: 124 GVQQNTFVVGAQVRESLVQSNDSDPDVIASFDTSGAELLYEIRLLSTEKRVAAAKYIVEN 183

Query: 183 RLDSKGAQDLARAMKDFPRRRGDKGWECFDYDSPGDCLAFMYYRLSREYNNPSEPRTVAL 242
           RLDSKGAQDLARA+KDFPRR+GD+GWECFDYDS GDCLA+MYYRLSREYNN SE RT AL
Sbjct: 184 RLDSKGAQDLARAIKDFPRRKGDRGWECFDYDSAGDCLAYMYYRLSREYNNSSERRTAAL 243

Query: 243 EDALKVVVTEKARDLIVGDLEGKGDGEEGDEEEIGAAVRVPVVRMKIGEVAEATTVVMMP 302
           E+ALKV VTEKAR+LIVGDLE KGDG++G EEE GA VRVPVVR+KIGEVAEAT VV+MP
Sbjct: 244 EEALKVGVTEKARNLIVGDLERKGDGKDGVEEETGAGVRVPVVRLKIGEVAEATKVVVMP 303

Query: 303 VCKAGKGEKGVGEAPMEVRSEGEFGVVVVEKEWRRWVVLPGWEPVAGLMKGGGVVVAFED 362
           VCKAG+GEKGVGEAPMEVRSEG+FGVVV EK W RWVVLPGWEPVAGL+KGGGVVVAF+D
Sbjct: 304 VCKAGEGEKGVGEAPMEVRSEGDFGVVVAEKGWSRWVVLPGWEPVAGLVKGGGVVVAFKD 363

Query: 363 ARALPWRVNRWYKEEPILVVADRNRKEVVAGDGFYLTGGTVG---GGGELKVERGNALME 422
           AR LPWRVNRWYKEEPILVVADR+RKEVVAGDGFYL GGTVG   GGGELKVERGNAL E
Sbjct: 364 ARVLPWRVNRWYKEEPILVVADRSRKEVVAGDGFYLMGGTVGGGDGGGELKVERGNALTE 423

Query: 423 MGVKESLGTVLLVVRPPKEMEDDQLSDEDWD 451
           MGVKESLGTV+LVVRPPKEMEDDQLSDEDWD
Sbjct: 424 MGVKESLGTVVLVVRPPKEMEDDQLSDEDWD 454

BLAST of HG10007864 vs. ExPASy TrEMBL
Match: A0A0A0LY43 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G690280 PE=4 SV=1)

HSP 1 Score: 762.3 bits (1967), Expect = 1.1e-216
Identity = 389/448 (86.83%), Postives = 414/448 (92.41%), Query Frame = 0

Query: 3   STTTTANSLTSLSAAAHTTATFLHPPRGSPLHLFLRRRPKPISAIINPFPPPLAAKQQVY 62
           ST T ANSL+SLS+ AHTT+ FLH PR SPL LFLRRR  PISAIINP    LAAKQQVY
Sbjct: 4   STITNANSLSSLSSIAHTTSIFLHQPRDSPLRLFLRRRLNPISAIINPSSSSLAAKQQVY 63

Query: 63  QPFRPPPSPLPSQYRSLDAEGKLNVLSNRLGLWFEYAPLISSLIQEGFTPPTLEEITGIS 122
           QPFRPPPSPLP QYRSLD EGKLN+LSNRLGLWFEYAPLISSL+QEGFTPP LEEITGIS
Sbjct: 64  QPFRPPPSPLPPQYRSLDTEGKLNILSNRLGLWFEYAPLISSLLQEGFTPPVLEEITGIS 123

Query: 123 GVQQNIFVVGAQVRESLVQSNNSDPDVIAAFDTSGAELLYELRLVSTEKRAAAAKYIVEN 182
           GVQQN F+VGAQVRESL+QSN+SDPDVIA+FDT GAELLYE+RL+STEKRAAAAKYIVEN
Sbjct: 124 GVQQNSFIVGAQVRESLLQSNDSDPDVIASFDTGGAELLYEIRLLSTEKRAAAAKYIVEN 183

Query: 183 RLDSKGAQDLARAMKDFPRRRGDKGWECFDYDSPGDCLAFMYYRLSREYNNPSEPRTVAL 242
           RLDSKGAQDLARAMKDFPRRRGDKGWE FDYD  GDCLA+MYYRLSREYN+ +E RT AL
Sbjct: 184 RLDSKGAQDLARAMKDFPRRRGDKGWEYFDYDFAGDCLAYMYYRLSREYNSSTERRTAAL 243

Query: 243 EDALKVVVTEKARDLIVGDLEGKGDGEEGDEEEIGAAVRVPVVRMKIGEVAEATTVVMMP 302
           E+ALKVVVTEKARDLIVGDLEGKGDG++G EEEIGAAV+VPVVRMKIGEVAEATTVV+MP
Sbjct: 244 EEALKVVVTEKARDLIVGDLEGKGDGKDGVEEEIGAAVKVPVVRMKIGEVAEATTVVVMP 303

Query: 303 VCKAGKGEKGVGEAPMEVRSEGEFGVVVVEKEWRRWVVLPGWEPVAGLMKGGGVVVAFED 362
           VCKAG+GEKGVGEAPMEVRSEGEFGVVV EK W RWVVLPGWEPVAGL+KGGGVVVAFED
Sbjct: 304 VCKAGEGEKGVGEAPMEVRSEGEFGVVVAEKGWSRWVVLPGWEPVAGLVKGGGVVVAFED 363

Query: 363 ARALPWRVNRWYKEEPILVVADRNRKEVVAGDGFYLTGGTVGGGGELKVERGNALMEMGV 422
           AR LPWRVNRWYKEEPILVVADR+R+EVVAGDGFYL GG   GGG+LKVERGNALMEMGV
Sbjct: 364 ARVLPWRVNRWYKEEPILVVADRSRREVVAGDGFYLMGGG-DGGGDLKVERGNALMEMGV 423

Query: 423 KESLGTVLLVVRPPKEMEDDQLSDEDWD 451
           KESLGTVLLVVRPP+E EDDQLSDEDWD
Sbjct: 424 KESLGTVLLVVRPPRETEDDQLSDEDWD 450

BLAST of HG10007864 vs. ExPASy TrEMBL
Match: A0A6J1F451 (rubisco accumulation factor 1.1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111439956 PE=4 SV=1)

HSP 1 Score: 680.2 bits (1754), Expect = 5.7e-192
Identity = 361/454 (79.52%), Postives = 396/454 (87.22%), Query Frame = 0

Query: 1   MLSTTTTANSLTSLSAAAHTTATF-LHPPRGSPLHLFLRRRPKPISAIIN--PFPPPLAA 60
           MLS T  A SL+  +AAAHTT TF LH P   PL LFLRR P PISA +N  P P PL A
Sbjct: 1   MLSATAPATSLS--AAAAHTTTTFLLHTP---PLRLFLRRPPNPISASLNPSPSPSPLVA 60

Query: 61  KQQVYQPFRPPPSPLPSQYRSLDAEGKLNVLSNRLGLWFEYAPLISSLIQEGFTPPTLEE 120
           K QVYQPFRPPPSPLPSQYRSLDA GKL+VL+NRLGLWFEYAPLISSLIQEGFTPPT+EE
Sbjct: 61  KPQVYQPFRPPPSPLPSQYRSLDAAGKLDVLANRLGLWFEYAPLISSLIQEGFTPPTIEE 120

Query: 121 ITGISGVQQNIFVVGAQVRESLVQS-NNSDPDVIAAFDTSGAELLYELRLVSTEKRAAAA 180
           ITGI GVQQN  VVGAQVRESLVQ+ N++DPD+IAAFDT GAELLYE+RL++ EKRAAAA
Sbjct: 121 ITGIGGVQQNTLVVGAQVRESLVQTINDADPDLIAAFDTGGAELLYEIRLLNAEKRAAAA 180

Query: 181 KYIVENRLDSKGAQDLARAMKDFPRRRGDKGWECFDYDSPGDCLAFMYYRLSREYNNPSE 240
           +YIVENRLD KGAQDLARAMKDFPRRRGDKGWE F+YD PGDCLAFM+YRLSREY+N SE
Sbjct: 181 RYIVENRLDGKGAQDLARAMKDFPRRRGDKGWESFNYDYPGDCLAFMHYRLSREYSNMSE 240

Query: 241 PRTVALEDALKVVVTEKARDLIVGDLEGKGDGEEGDEEEIGAAVRVPVVRMKIGEVAEAT 300
            RT ALEDALKV VTE+AR LIV DLE +G+G++  EEEI A VRVPVVR+KIGEVAEAT
Sbjct: 241 ARTAALEDALKVAVTEEARSLIVRDLEREGEGKDEVEEEIEAGVRVPVVRLKIGEVAEAT 300

Query: 301 TVVMMPVCKAGKGEKGVGEAPMEVRSEGEFGVVVVEKEWRRWVVLPGWEPVAGLMKGGGV 360
           TVV++PVC   +G KGVGEAPMEVRSEGEFGVVV E+ WRRWVVLPGWEP+AGL K GGV
Sbjct: 301 TVVVLPVCNGEEGGKGVGEAPMEVRSEGEFGVVVAEEGWRRWVVLPGWEPIAGLGK-GGV 360

Query: 361 VVAFEDARALPWRVNRWYKEEPILVVADRNRKEVVAGDGFYLTGGTVGGGGELKVERGNA 420
           VVAF+DARALPWRVNRWYKEE ILVVADR+RKEV AGDGFYLTG T G  GELKV+RG+ 
Sbjct: 361 VVAFKDARALPWRVNRWYKEEAILVVADRSRKEVEAGDGFYLTGAT-GSDGELKVQRGSE 420

Query: 421 LMEMGVKESLGTVLLVVRPPKEMEDDQLSDEDWD 451
           LMEMG+KESLGTVLLVVRPPK+M+DDQLSDEDWD
Sbjct: 421 LMEMGLKESLGTVLLVVRPPKDMDDDQLSDEDWD 447

BLAST of HG10007864 vs. TAIR 10
Match: AT5G28500.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast stroma, chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G04550.1); Has 109 Blast hits to 109 proteins in 49 species: Archae - 0; Bacteria - 67; Metazoa - 0; Fungi - 0; Plants - 41; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 446.8 bits (1148), Expect = 2.0e-125
Identity = 249/449 (55.46%), Postives = 321/449 (71.49%), Query Frame = 0

Query: 11  LTSLSAAAHTTATFLHPPRGSPLHLFLRRRP---KPISAIINPFPP----PLAAKQQVYQ 70
           + SL+A   +++ F      S  H F   RP   KP + I +   P        KQQ+YQ
Sbjct: 1   MLSLTATTLSSSIFTQ----SKTHGFFNTRPVYRKPFTTITSALIPASNRQAPPKQQLYQ 60

Query: 71  PFRPPPSPLPSQYRSLDAEGKLNVLSNRLGLWFEYAPLISSLIQEGFTPPTLEEITGISG 130
           PFRPPPSP+P ++RSLD  GK+ VL++RLGLWFEYAPLISSL  EGFTPP++EE+TGISG
Sbjct: 61  PFRPPPSPIPPKFRSLDTAGKIEVLADRLGLWFEYAPLISSLYTEGFTPPSIEELTGISG 120

Query: 131 VQQNIFVVGAQVRESLVQSNNSDPDVIAAFDTSGAELLYELRLVSTEKRAAAAKYIVENR 190
           V+QN  +VGAQVR+SLVQS  + P++IAAFDT+GAELLYE+RL++T +R AAA+YIV++ 
Sbjct: 121 VEQNSLIVGAQVRDSLVQS-GAKPELIAAFDTNGAELLYEIRLLNTTQRVAAAEYIVDHG 180

Query: 191 LDSKGAQDLARAMKDFPRRRGDKGWECFDYDSPGDCLAFMYYRLSREYNNPSEPRTVALE 250
            D+KGA DLARA+KDFP RRGD G   FDY+ PGDCL+FM YR SRE+ +PSE RT  LE
Sbjct: 181 FDTKGAGDLARAIKDFPHRRGDVGLGDFDYNLPGDCLSFMLYRKSREHRSPSEIRTTLLE 240

Query: 251 DALKVVVTEKARDLIVGDLEGKGDGEEGDEEEIGAAVRVPVVRMKIGEVAEATTVVMMPV 310
            AL+  VTEKA+  ++ +L G+ + E   EEEI   +RVPVVR++ GEVA A++VV++PV
Sbjct: 241 QALETAVTEKAKKAVLRELHGESEEERVKEEEI-KIIRVPVVRLRFGEVAGASSVVVLPV 300

Query: 311 CKAGKGEKGVGEAPMEVRSEGEFGVVVVEKEWRRWVVLPGWEPVAGLMKGGGVVVAFEDA 370
           CKA +GE+ + EAPME  S GEFGVV  EK+W RWVVLPGW+PV  + K  GV V+F D 
Sbjct: 301 CKAEEGEEKLLEAPMEFESGGEFGVVEAEKDWSRWVVLPGWDPVVAVRK--GVAVSFSDD 360

Query: 371 R-ALPWRVNRWYKEEPILVVADRNRKEVVAGDGFYLTGGTVGGGGELKVERGNALMEMGV 430
           R  LPW      K E I+VV DR +K V A +G+Y     V   G +K++RG  L E GV
Sbjct: 361 REVLPWN----GKGEAIMVVIDREKKTVEADNGYYY---LVVADGGMKLDRGLVLKEKGV 420

Query: 431 KESLGTVLLVVRPPKEMEDD-QLSDEDWD 451
            ESLG V+LVVRPP++ +D+ Q++DEDWD
Sbjct: 421 NESLGMVVLVVRPPRDDDDEWQINDEDWD 434

BLAST of HG10007864 vs. TAIR 10
Match: AT3G04550.1 (unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast stroma, chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G28500.1); Has 110 Blast hits to 110 proteins in 51 species: Archae - 0; Bacteria - 67; Metazoa - 1; Fungi - 0; Plants - 41; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 443.4 bits (1139), Expect = 2.3e-124
Identity = 236/415 (56.87%), Postives = 307/415 (73.98%), Query Frame = 0

Query: 41  PKPISAIINPFPPPLAAKQQVYQPFRPPPSPLPSQYRSLDAEGKLNVLSNRLGLWFEYAP 100
           PK  SA + P  PP  A+QQ+YQPFRPP SP+P+Q+RSLD+ GK+ +L+ R+ LWFEYAP
Sbjct: 46  PKRSSANMIPKNPP--ARQQLYQPFRPPSSPIPTQFRSLDSAGKIEILAGRMALWFEYAP 105

Query: 101 LISSLIQEGFTPPTLEEITGISGVQQNIFVVGAQVRESLVQSNNSDPDVIAAFDTSGAEL 160
           LISSL  +GFTPPT+EE+TGIS ++QN  +VGAQVR+S++QS + +P++I+AFDT GAEL
Sbjct: 106 LISSLYTDGFTPPTIEELTGISSIEQNRLIVGAQVRDSILQSIH-EPELISAFDTGGAEL 165

Query: 161 LYELRLVSTEKRAAAAKYIVENRLDSKGAQDLARAMKDFPRRRGDKGWECFDYDSPGDCL 220
           LYE+RL+ST +R AAA +I++  +DSKGAQDLARA+KD+P RRGD GW  FDY+ PGDCL
Sbjct: 166 LYEIRLLSTTQRVAAATFIIDRNIDSKGAQDLARAIKDYPNRRGDVGWLDFDYNLPGDCL 225

Query: 221 AFMYYRLSREYNNPSEPRTVALEDALKVVVTEKARDL----IVGDLEGKGDGEEGDEEEI 280
           +F+YYR SRE  NPS+ RT  L  AL V  +EKA++     + GD E + + E+  +EE 
Sbjct: 226 SFLYYRQSRENKNPSDQRTSMLLQALGVAESEKAKNRLNTELYGDKEAEKEKEKKKKEEE 285

Query: 281 GAAVRVPVVRMKIGEVAEATTVVMMPVCKAGKGEKGVGEAPMEVRSEGEFGVVVVEKEWR 340
             A+R+PVVR+K GEVAEAT+VV++PVCKA +GEK + EAPME+ + G+F VV  EK W+
Sbjct: 286 VKAIRIPVVRLKFGEVAEATSVVVLPVCKAEEGEKKILEAPMEIIAGGDFKVVEAEKGWK 345

Query: 341 RWVVLPGWEPVAGLMKGGGVVVAFEDARALPWRVNRWYKEEPILVVADRNRKEVVAGDGF 400
           RWVVLP W PVA + KGG  V   +D + LPW      KEEP+LVVADR R  V A DG+
Sbjct: 346 RWVVLPSWNPVAAIGKGGVAVSFRDDRKVLPWD----GKEEPLLVVADRVRNVVEADDGY 405

Query: 401 YLTGGTVGGGGELKVERGNALMEMGVKESLGTVLLVVRPPKEMEDD-QLSDEDWD 451
           YL     G    LK+E+G+ L    VKESLG V+LVVRPP+E +DD Q S ++WD
Sbjct: 406 YLVVAENG----LKLEKGSDLKAREVKESLGMVVLVVRPPREDDDDWQTSHQNWD 449

BLAST of HG10007864 vs. TAIR 10
Match: AT5G28500.2 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G04550.1). )

HSP 1 Score: 285.4 bits (729), Expect = 7.8e-77
Identity = 153/271 (56.46%), Postives = 198/271 (73.06%), Query Frame = 0

Query: 11  LTSLSAAAHTTATFLHPPRGSPLHLFLRRRP---KPISAIINPFPP----PLAAKQQVYQ 70
           + SL+A   +++ F      S  H F   RP   KP + I +   P        KQQ+YQ
Sbjct: 1   MLSLTATTLSSSIFTQ----SKTHGFFNTRPVYRKPFTTITSALIPASNRQAPPKQQLYQ 60

Query: 71  PFRPPPSPLPSQYRSLDAEGKLNVLSNRLGLWFEYAPLISSLIQEGFTPPTLEEITGISG 130
           PFRPPPSP+P ++RSLD  GK+ VL++RLGLWFEYAPLISSL  EGFTPP++EE+TGISG
Sbjct: 61  PFRPPPSPIPPKFRSLDTAGKIEVLADRLGLWFEYAPLISSLYTEGFTPPSIEELTGISG 120

Query: 131 VQQNIFVVGAQVRESLVQSNNSDPDVIAAFDTSGAELLYELRLVSTEKRAAAAKYIVENR 190
           V+QN  +VGAQVR+SLVQS  + P++IAAFDT+GAELLYE+RL++T +R AAA+YIV++ 
Sbjct: 121 VEQNSLIVGAQVRDSLVQS-GAKPELIAAFDTNGAELLYEIRLLNTTQRVAAAEYIVDHG 180

Query: 191 LDSKGAQDLARAMKDFPRRRGDKGWECFDYDSPGDCLAFMYYRLSREYNNPSEPRTVALE 250
            D+KGA DLARA+KDFP RRGD G   FDY+ PGDCL+FM YR SRE+ +PSE RT  LE
Sbjct: 181 FDTKGAGDLARAIKDFPHRRGDVGLGDFDYNLPGDCLSFMLYRKSREHRSPSEIRTTLLE 240

Query: 251 DALKVVVTEKARDLIVGDLEGKGDGEEGDEE 275
            AL+  VTEKA+  ++ +L G+ + E G E+
Sbjct: 241 QALETAVTEKAKKAVLRELHGESEEERGKED 266