HG10007851 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10007851
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionpalmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like
LocationChr10: 15780738 .. 15785766 (+)
RNA-Seq ExpressionHG10007851
SyntenyHG10007851
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGTCTCAATAGAACCAAGGGAAGTAGAAAAGTCTCCTACATCATTTGGGAAGGAAGAATGGAATGAGATAGACAAAGAAATGGCTTTCATTATTTTCTTCTTGCATGTCCTTTGTATTTTTGCACCTTTTCACTTTAATTGGAGTGCATTACGGGTCGCCATGGTATTATACGTTATCACCGGTCTGTTTGGTATAAGTCTCTCATATCATAGAAATCTTTCGCACAAGAGTTTCAAGCTTCCGAAATGGCTTGAGTACTTGTTTGCTTACTGTGGAGTTCAAGCACTTGAGGTCAGTCAAATGATAAACTCTAAAGCAGGTTAAAATATCTCGTATTTTAAAAAAAAAATTATTAATTGAACTGTTTGTATATACTTTAGGGAAATCCAATCGATTGGGTAAGTATGCATAGATATCACCACCAAGTTGCTGATACGGAAAAAGATCCACAACGTCCTGATCGAAAATTTTTGTGGCTTTCTAATTACTATGCTTCAACTAAAATAGTTAATTTGAGGTACTTTGTCATTTTTCAAGAGATAGAAAAAAACATATTTTTAATGGTTAGGAAACAAGGGAGAAGAGATAATGTTGGGGACTTGGAGAAGCAAATGTTCTACAAATTCATTCACAAAACATATCTTCTTCATCCAATTGCTCTTGCAATCTTGCTATATGTTGTTGGAGGAATGCCTTTCTTTATATGGGGAATGGTAAGTAAATAAACTCTCTATTTTGTTTACTTCAAATTACTCAATTTATTATAACAAAAATATGAAAAATAGATCACCAAAGAAAATATGTATTTGGTCTTTTGAATTTAGATTACGGTTTCTATTCGATCTATAGATTTTGAAATCTTATATTTTGACATTAATTTTAAAAAATTATGATTTGGTATTTTATTAATTAATAATTTTAATAGTGATGTTACAAATTATAAAATACTTTTTGTCCTTAAACTTTCAAAGTAACAATTTAGGCCCTAATTAACTATATTGGTTTGGAGTGATTCTTATCGGTACTTTCAAATTTGTAGCAACTGATTTCTAAATTTTAATTAGTAACAATTTAATTTCTAAACTTAATAAGTAATAATTTTAGTTTTTTAGTTTTAGTTTTTAAAAATTAAGTTTAAAAAATTAACAACTTGGTCTTTATACTTCCACTTATTGATATCTTTGTTTAGTTATCTACTTTTTATGAGTTTCTTAAAAAACCAAGTCAAGTTTTGAAAACTAAAAAAATAGTTTTGATAACTACTTTTTTTAGTTTTCAAAATTTTGGATTGGTTTTTGAAAATATTGTAAAAGTAGACAATAAAGTAAGAAATTTAGAGCGGGAAGAAGGGTTTATAGGCTTAATTTTCAAAACCAAAAACAAAAAATTAAGACTTCATTTGATAACCATTTCGTTGTTGGTTTCTAACTTTTAAAAATTAAGCCTATTTCCTCACAATTTCTTACAATGATTTGTATATTTCTTAAGTATAAGAGTTGCATTCTTATCTAAATTTCAAAAAGAAAAATAATATTTTAAAAACTATTTTTTTTTAGTTTTCAAATTATGGCTTGATTTTTGAAAATATTAGCAAAATATAGATAACAAAACAAAAAAATTTAGAGGTGGAAGGAATGCTTATAGGCTTTTTTTTTTCTAAAACTAAAAACAAAAAATCAAATGGTTATCAAACAACGCATAAATGATTACCAAATGAGGTCAAAGTTTTTTTAATCGTGTTTTATTTTTAAAATTTGACTTAGAATTAAAGTGTTTAGTTAAAAAAGATGAAAGTCGTGGTTAAGAAGTAGTAAAGACAACAATGATAATTTTCAAAAACCAAGAACAAAATAAATAAATAAAAATCAAATGGTTACCTAACGAAACCTACGTTTTTCTAATAGTAATTTAGATAAGCAAATAGGTTCAACATTAAATTATAAGTTTAGTCAAGTTTGAGACCAATATGTGTGCTTGAACTTTAAAAGTGACTTATACTCGTGGTAGAAAATTTCCATTGAAATCTCGAATTTCGATTACAATTTAAGGATTTCGATGACCTTAAAATTTCAGTACGGGAAGATTTCTCAAAATTTCTAGAAAATTTTGAGGAATTTTTTATTTTGTTTGTCTCCTATTTTGACGAAATTTTGATAAATCTCGAAATTTCGAGAATATTTTATTTTATTCTATTTTGTAGAATTAGATATATTAGCTTGGTTCAAGTTTTTCAATTTTATGGTCCTGATTGTACACAATTGTATAGTTTCACACACTAATATCATTTTTCAACGAAGAATGAAATATTTCAATGTGCTACTTTTTTTAATTTCCAACTTTTCTCAACTTTTAGCTTTATTTTCAATCTAAAACTTTCATTTCTAAAGAAATTTAATTTGGAAAAACCATAAAAAAATCTATATTTATTTTTAACCTCATACAAATTTTTTTCATTAAATTTTCACATCATTTCAACTCAAAATTGAATTGATATGAGTTATTTTTCTTTTACCATCCAAAATTATCATTTTCACCTCAAAATGTTCCTAAAGTACTTAATATTATTTTGTAATTATTTTCCCATTTCTTATTTAAGATTTTGCTCTCGCATCGACATTTACAATATACATCTAAATATTGTCTTACTTCATATAAATAAGGATTAAAAATGGTCAATTATAATATAATTAAGCCACCATCAAAGTTTTATTAACTAATTATAATAATGTCCATCAATTTTCATAAAGTTTCCCAAAATCTCCATCCATATCTTTATAGAATCGAGACCTCGATATTTCAAACCTTACTTATACCTACCTAGCACTCAATTTTACGTCTAATAAGTCCTTGACCTTTTGAATAATTTAATAAGTTCTTAAACTTTCAAGTATGTGTCATAGATCTATAGCATGAAATTTTAAAAATGATTGACTTATTAGACATAAAATTTAATTTTTCATCTAATAAGATATTTTAGACTTTTAATTTTGTTTCTATCAAATTCGTAAATTTTAAAAATTTTCAAATATGTTAACAACCTCTATGATATAGAAAGTTAAAAATTGAATTAATTATTCTACGCGAGCTTGACAATTATTAAAATTATATGACCTATTATACACCAAATTTAATATATATATATATATATATATATATATATATATATATATATATATATATTAGTCTCTTTTAATAAAATTCCAGACCTAATTAGACATAAACTTAAAATTGAAAGACTAGACATATGGTTTAACTGAAGTCCTCACTGTTCATTTATATATTTCTTTATTTTTTCATATATTTCTTTATTTATTTTAAGGAAGGAGAAGGATGAGCCAATGGCCACCAAGCTCGTCTTAGTGATTAAAAAAGATTACAACAAAATTGTTATAGGTTCAAATTATAATTTTAAGTTTTATTTTGAAAATTGATTATCTAGATAGTGCTTAAAAATATAAAGAAATTAGAAAAATTATTATAAATGAAAATATATCAAATTATTTATAAATATAGAAATTTTTTTCTATTTATTAGTGATAGACTTCTATCGCTCAATAAATTTTTATCTTAGTCTATCACTTATTGATAAAAGTCTATCGTTGATAGACAGTGAAATTTTTTTATATTTATAAATATTTTGACTCATTTTACTATATTTGAAAATAGTTCAAAGAAATTAAAAGAAGATTGACATTATTATTGTATTTATCATGCAGTGTGTGAGAACCGTCGTAGTGTTACACACATCATTTATGGTAAATTCAATATGTCATAGATCGGGAAAGCAACAATGGAAGACTCGTGATTTGTCAATAAACAATGGGTAAGTGTGTTGGTTGTTTGGTTGCACTACATTTAAAAGTAAAATTCTATTAACCATGATTATTATTATTTTTTTCCATTGTAGTGTGATTTTCACGGTGCTGTCACTAACACTAAATTTTAAAACAACTTAATAATTTAGTCTCTCAATTATAATTAGTAATAATTTAGTCAATGTACTTCATAACTTATTATTTATAATTTATAAAAAAAAATATCACAAATCGTTTATTAAAAAAAAGAGAAAAAAATCACAAATTGTTTATTAATCTACATATATAATTTTTTTTTTATTAAATCCTACCAAAAAAAAGTTTTACAATTAAGGGTTAAACCGTTTCAAATTATAAAGTACAAGGATTGTCACTTATTAAAGTTCAGGGACTAAATTGTTACAAATTTAAAAATATAGAGATTAAACTTCAAGCATTAAATTGTTACTTTCATAAAAGTTCAGAGACTAAAAGTGTTTTTAATTTTTCATATATTACCAAACACTATAGAATTAGTAATTCATTGAAACATGTTTTTTTAAAAAAATATATCAATTTATATCTCTAAACTTTCATTAGCCAAATTTAGAAACTCAATTAGGATTTTTCTATGAAAATCGTCTATGCATCAATTCAAATCATCCATTTTATTTAATGCAACATTAAAAAGAAGTATACACATACAAAAATTGATACACTGACGATTTTCAAAAACAATCTTAAAAAAGTCTAGAATATTATTATTAATTCACGTATGATAACTTAGAAATTTAATTGAAACCAATTATAAGTCATTTCACACGATATTTTTCAAATGAATTTGAAATGAGTATGTTAAATTAATACACTTACAAAAATTTAAAGTTTAAATTGATACAATTATTAGTTTATGATTTTGATGGATATAACTCCAAAATTTAAAAATATAAATTGATATATATTTCTCCCTAAATAAAATAATATTGTTATAAAGAGAGTAATTTATAATAATTGATTTTATTGTGTTTTTAAGGTGGATGAGTTTGGTTACATTTGGAGATAGTTGGCATAATAATCACCATGCCTTTCCTTATTCAGCTCAACATGGATTTGAATGGTGGCAGATTGATTTTAGTTGGTATCTTATTGTTTTTCTTCAAGCCATTGGAGTTGCTACTAATGTTAAATTGCCCCCTCATAAAGCCACACTAGGTAAATTAAAGCTTTGA

mRNA sequence

ATGGATGTCTCAATAGAACCAAGGGAAGTAGAAAAGTCTCCTACATCATTTGGGAAGGAAGAATGGAATGAGATAGACAAAGAAATGGCTTTCATTATTTTCTTCTTGCATGTCCTTTGTATTTTTGCACCTTTTCACTTTAATTGGAGTGCATTACGGGTCGCCATGGTATTATACGTTATCACCGGTCTGTTTGGTATAAGTCTCTCATATCATAGAAATCTTTCGCACAAGAGTTTCAAGCTTCCGAAATGGCTTGAGTACTTGTTTGCTTACTGTGGAGTTCAAGCACTTGAGGGAAATCCAATCGATTGGGTAAGTATGCATAGATATCACCACCAAGTTGCTGATACGGAAAAAGATCCACAACGTCCTGATCGAAAATTTTTGTGGCTTTCTAATTACTATGCTTCAACTAAAATAGTTAATTTGAGGTACTTTGTCATTTTTCAAGAGATAGAAAAAAACATATTTTTAATGGTTAGGAAACAAGGGAGAAGAGATAATGTTGGGGACTTGGAGAAGCAAATGTTCTACAAATTCATTCACAAAACATATCTTCTTCATCCAATTGCTCTTGCAATCTTGCTATATGTTGTTGGAGGAATGCCTTTCTTTATATGGGGAATGTGTGTGAGAACCGTCGTAGTGTTACACACATCATTTATGGTAAATTCAATATGTCATAGATCGGGAAAGCAACAATGGAAGACTCGTGATTTGTCAATAAACAATGGGTGGATGAGTTTGGTTACATTTGGAGATAGTTGGCATAATAATCACCATGCCTTTCCTTATTCAGCTCAACATGGATTTGAATGGTGGCAGATTGATTTTAGTTGGTATCTTATTGTTTTTCTTCAAGCCATTGGAGTTGCTACTAATGTTAAATTGCCCCCTCATAAAGCCACACTAGGTAAATTAAAGCTTTGA

Coding sequence (CDS)

ATGGATGTCTCAATAGAACCAAGGGAAGTAGAAAAGTCTCCTACATCATTTGGGAAGGAAGAATGGAATGAGATAGACAAAGAAATGGCTTTCATTATTTTCTTCTTGCATGTCCTTTGTATTTTTGCACCTTTTCACTTTAATTGGAGTGCATTACGGGTCGCCATGGTATTATACGTTATCACCGGTCTGTTTGGTATAAGTCTCTCATATCATAGAAATCTTTCGCACAAGAGTTTCAAGCTTCCGAAATGGCTTGAGTACTTGTTTGCTTACTGTGGAGTTCAAGCACTTGAGGGAAATCCAATCGATTGGGTAAGTATGCATAGATATCACCACCAAGTTGCTGATACGGAAAAAGATCCACAACGTCCTGATCGAAAATTTTTGTGGCTTTCTAATTACTATGCTTCAACTAAAATAGTTAATTTGAGGTACTTTGTCATTTTTCAAGAGATAGAAAAAAACATATTTTTAATGGTTAGGAAACAAGGGAGAAGAGATAATGTTGGGGACTTGGAGAAGCAAATGTTCTACAAATTCATTCACAAAACATATCTTCTTCATCCAATTGCTCTTGCAATCTTGCTATATGTTGTTGGAGGAATGCCTTTCTTTATATGGGGAATGTGTGTGAGAACCGTCGTAGTGTTACACACATCATTTATGGTAAATTCAATATGTCATAGATCGGGAAAGCAACAATGGAAGACTCGTGATTTGTCAATAAACAATGGGTGGATGAGTTTGGTTACATTTGGAGATAGTTGGCATAATAATCACCATGCCTTTCCTTATTCAGCTCAACATGGATTTGAATGGTGGCAGATTGATTTTAGTTGGTATCTTATTGTTTTTCTTCAAGCCATTGGAGTTGCTACTAATGTTAAATTGCCCCCTCATAAAGCCACACTAGGTAAATTAAAGCTTTGA

Protein sequence

MDVSIEPREVEKSPTSFGKEEWNEIDKEMAFIIFFLHVLCIFAPFHFNWSALRVAMVLYVITGLFGISLSYHRNLSHKSFKLPKWLEYLFAYCGVQALEGNPIDWVSMHRYHHQVADTEKDPQRPDRKFLWLSNYYASTKIVNLRYFVIFQEIEKNIFLMVRKQGRRDNVGDLEKQMFYKFIHKTYLLHPIALAILLYVVGGMPFFIWGMCVRTVVVLHTSFMVNSICHRSGKQQWKTRDLSINNGWMSLVTFGDSWHNNHHAFPYSAQHGFEWWQIDFSWYLIVFLQAIGVATNVKLPPHKATLGKLKL
Homology
BLAST of HG10007851 vs. NCBI nr
Match: XP_038879870.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 535.8 bits (1379), Expect = 2.5e-148
Identity = 256/304 (84.21%), Postives = 276/304 (90.79%), Query Frame = 0

Query: 1   MDVSIEPREVEKSPTSFGKEEWNEIDKEMAFIIFFLHVLCIFAPFHFNWSALRVAMVLYV 60
           MDVSIEPRE+EK   S GKEEW EIDKEMAFIIF LHVLCIFAPF FNWSAL VA +LY 
Sbjct: 1   MDVSIEPREMEKPLKSSGKEEWIEIDKEMAFIIFLLHVLCIFAPFQFNWSALWVATMLYF 60

Query: 61  ITGLFGISLSYHRNLSHKSFKLPKWLEYLFAYCGVQALEGNPIDWVSMHRYHHQVADTEK 120
           I+GLFG+SLSYHRNL+H+SFKLPKWLEYLFAYCGVQALEGNPIDWVSMHRYHH+VADT+K
Sbjct: 61  ISGLFGLSLSYHRNLAHRSFKLPKWLEYLFAYCGVQALEGNPIDWVSMHRYHHEVADTDK 120

Query: 121 DPQRPDRKFLWLSNYYASTKIVNL-RYFVIFQEIEKNIFLMVRKQGRRDNVGDLEKQMFY 180
           DPQR D++F W  NYYASTK VN  RYFVIFQEIEKNIFLMVRKQ RRDNV DLEKQ+FY
Sbjct: 121 DPQRFDQRFSWFPNYYASTKRVNRPRYFVIFQEIEKNIFLMVRKQVRRDNVRDLEKQIFY 180

Query: 181 KFIHKTYLLHPIALAILLYVVGGMPFFIWGMCVRTVVVLHTSFMVNSICHRSGKQQWKTR 240
           KFIHKTYLLHPIALAILLYVVGGMPF IWGM VR VVVLHT+ MVNSICH SGKQQWKTR
Sbjct: 181 KFIHKTYLLHPIALAILLYVVGGMPFLIWGMGVRNVVVLHTTLMVNSICHGSGKQQWKTR 240

Query: 241 DLSINNGWMSLVTFGDSWHNNHHAFPYSAQHGFEWWQIDFSWYLIVFLQAIGVATNVKLP 300
           DLS+N GW+SL++FG+SWHNNHHAFPYSA+HGF+WWQIDFSWY+I FLQ IGVATNVKL 
Sbjct: 241 DLSLNVGWVSLISFGESWHNNHHAFPYSARHGFKWWQIDFSWYVINFLQVIGVATNVKLA 300

Query: 301 PHKA 304
           PHKA
Sbjct: 301 PHKA 304

BLAST of HG10007851 vs. NCBI nr
Match: XP_022988176.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 471.9 bits (1213), Expect = 4.4e-129
Identity = 223/300 (74.33%), Postives = 247/300 (82.33%), Query Frame = 0

Query: 1   MDVSIEPREVEKSPTSFGKEEWNEIDKEMAFIIFFLHVLCIFAPFHFNWSALRVAMVLYV 60
           MDV IEP +  K P S GKEEW +IDK+ A  IF +HVLCIFAPF  N  A+RVA+ L+ 
Sbjct: 1   MDVPIEPMDTAKPPRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRAIRVALALHF 60

Query: 61  ITGLFGISLSYHRNLSHKSFKLPKWLEYLFAYCGVQALEGNPIDWVSMHRYHHQVADTEK 120
           ITG FGI+LSYHRNLSH+SFKLPKW EYLFA CGV  L G+PI+WVSMHRYHHQVADTEK
Sbjct: 61  ITGFFGITLSYHRNLSHRSFKLPKWFEYLFALCGVHTLLGDPINWVSMHRYHHQVADTEK 120

Query: 121 DPQRPDRKFLWLSNYYASTKIVNLRYFVIFQEIEKNIFLMVRKQGRRDNVGDLEKQMFYK 180
           DPQR  R+FLWLSNY+ASTK V  RY  I Q IEKNIFLM+RK  +RDNVGDLE Q FYK
Sbjct: 121 DPQRTPRRFLWLSNYFASTKRVGPRYLCILQGIEKNIFLMIRKFVKRDNVGDLENQSFYK 180

Query: 181 FIHKTYLLHPIALAILLYVVGGMPFFIWGMCVRTVVVLHTSFMVNSICHRSGKQQWKTRD 240
           FIHKTYLLHPI LAILLYVVGGMPF +WGM VRTV+VLH S MVN+ICHR GK+ WKT D
Sbjct: 181 FIHKTYLLHPIGLAILLYVVGGMPFLVWGMGVRTVLVLHLSLMVNTICHRFGKRSWKTND 240

Query: 241 LSINNGWMSLVTFGDSWHNNHHAFPYSAQHGFEWWQIDFSWYLIVFLQAIGVATNVKLPP 300
            S NNG M L++FG+ WHNNHHAFPYSA+HGFEWWQID SWY I FLQAIGVAT+VKLPP
Sbjct: 241 SSRNNGLMGLISFGEGWHNNHHAFPYSARHGFEWWQIDTSWYAIRFLQAIGVATDVKLPP 300

BLAST of HG10007851 vs. NCBI nr
Match: XP_022961086.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 456.8 bits (1174), Expect = 1.5e-124
Identity = 214/289 (74.05%), Postives = 240/289 (83.04%), Query Frame = 0

Query: 12  KSPTSFGKEEWNEIDKEMAFIIFFLHVLCIFAPFHFNWSALRVAMVLYVITGLFGISLSY 71
           K   S GKEEW +IDK+ A  IF +HVLCIFAPF  N   LR+A+ L+ ITG FGI+LSY
Sbjct: 5   KPRRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRVLRIALALHFITGFFGITLSY 64

Query: 72  HRNLSHKSFKLPKWLEYLFAYCGVQALEGNPIDWVSMHRYHHQVADTEKDPQRPDRKFLW 131
           HRNLSH+SFKLPKWLEYLFA CG   L G+PI+WVSMHRYHHQVADTEKDPQR  R+FLW
Sbjct: 65  HRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWVSMHRYHHQVADTEKDPQRTPRRFLW 124

Query: 132 LSNYYASTKIVNLRYFVIFQEIEKNIFLMVRKQGRRDNVGDLEKQMFYKFIHKTYLLHPI 191
           LSNY+ASTK V  RY  I + IEKNIFLM+R+  +RDNVGDLE Q FYKFIHKTYLLHPI
Sbjct: 125 LSNYFASTKRVGPRYLFILEGIEKNIFLMIREFVKRDNVGDLENQSFYKFIHKTYLLHPI 184

Query: 192 ALAILLYVVGGMPFFIWGMCVRTVVVLHTSFMVNSICHRSGKQQWKTRDLSINNGWMSLV 251
            LAILLYVVGGMPF +WGM VRTV+VLH S MVN+ICHR GK+QWKT D S NNG MSL+
Sbjct: 185 GLAILLYVVGGMPFLVWGMGVRTVLVLHLSLMVNTICHRFGKRQWKTNDSSRNNGLMSLI 244

Query: 252 TFGDSWHNNHHAFPYSAQHGFEWWQIDFSWYLIVFLQAIGVATNVKLPP 301
           +FG+ WHNNHHAFPYSA+HGFEWWQ+D SWY I FLQAIGVAT+VKLPP
Sbjct: 245 SFGEGWHNNHHAFPYSARHGFEWWQVDTSWYAIRFLQAIGVATDVKLPP 293

BLAST of HG10007851 vs. NCBI nr
Match: XP_023533044.1 (acyl-CoA C20 Delta5-desaturase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 453.8 bits (1166), Expect = 1.2e-123
Identity = 212/298 (71.14%), Postives = 243/298 (81.54%), Query Frame = 0

Query: 3   VSIEPREVEKSPTSFGKEEWNEIDKEMAFIIFFLHVLCIFAPFHFNWSALRVAMVLYVIT 62
           V +EP +    P S GKEEW +IDK+ A  IF +HVLCIFAPF  N  AL++A+ L+ IT
Sbjct: 83  VPLEPMDTPNPPRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRALQIALALHFIT 142

Query: 63  GLFGISLSYHRNLSHKSFKLPKWLEYLFAYCGVQALEGNPIDWVSMHRYHHQVADTEKDP 122
           G FG++LSYHRNLSH+SFKLPKWLEYLFA CG   L G+PI+WVSMHRYHHQVADTEKDP
Sbjct: 143 GFFGVTLSYHRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWVSMHRYHHQVADTEKDP 202

Query: 123 QRPDRKFLWLSNYYASTKIVNLRYFVIFQEIEKNIFLMVRKQGRRDNVGDLEKQMFYKFI 182
           QR  R+FLWLSNY+ STK V  RY  I + IEKNIFLM+R+  +RDNVGDLE Q FYKFI
Sbjct: 203 QRTPRRFLWLSNYFGSTKRVGPRYLCILEGIEKNIFLMIREFVKRDNVGDLENQSFYKFI 262

Query: 183 HKTYLLHPIALAILLYVVGGMPFFIWGMCVRTVVVLHTSFMVNSICHRSGKQQWKTRDLS 242
           HKTYLLHPI LAILLYVVGGMP  +WGM VRTV+VLH S MVN+ICHR GK+QWKT D S
Sbjct: 263 HKTYLLHPIGLAILLYVVGGMPSLVWGMGVRTVLVLHLSLMVNTICHRFGKRQWKTNDSS 322

Query: 243 INNGWMSLVTFGDSWHNNHHAFPYSAQHGFEWWQIDFSWYLIVFLQAIGVATNVKLPP 301
            NNG MSL++FG+ WHNNHHAF YSA+HGFEWWQ+D SWY I FLQAIGVA++VKLPP
Sbjct: 323 RNNGLMSLISFGEGWHNNHHAFLYSARHGFEWWQVDTSWYAIRFLQAIGVASDVKLPP 380

BLAST of HG10007851 vs. NCBI nr
Match: XP_022153644.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 422.2 bits (1084), Expect = 4.0e-114
Identity = 191/299 (63.88%), Postives = 238/299 (79.60%), Query Frame = 0

Query: 6   EPREVEKSPTSFGKEEWNEIDKEMAFIIFFLHVLCIFAPFHFNWSALRVAMVLYVITGLF 65
           EP+E+ K P  + + +W ++DK++A  +F +H+LCI APF FNWSA  VA VLY++TGLF
Sbjct: 7   EPKEMMKPPRPYERRKWTKLDKDIACSLFLVHLLCILAPFQFNWSAFWVAFVLYIVTGLF 66

Query: 66  GISLSYHRNLSHKSFKLPKWLEYLFAYCGVQALEGNPIDWVSMHRYHHQVADTEKDPQRP 125
           G++LSYHRNL+HKSFKLPKWLEYLFAYCGVQAL+G+P+DWVS HR HHQ  DTE+DP  P
Sbjct: 67  GMTLSYHRNLAHKSFKLPKWLEYLFAYCGVQALQGDPMDWVSTHRCHHQFVDTERDPHSP 126

Query: 126 DRKF-----LWLSNYYASTKIVNLRYFVIFQEIEKNIFLMVRKQGRRDNVGDLEKQMFYK 185
            R F     LWL + Y  TK V  +YF  FQ++E+NIF++  K G+ +NV DLEKQ FY+
Sbjct: 127 IRGFWFSHILWLFDSYNLTKKVCPKYFSNFQKLERNIFVLFSKHGKPENVADLEKQAFYR 186

Query: 186 FIHKTYLLHPIALAILLYVVGGMPFFIWGMCVRTVVVLHTSFMVNSICHRSGKQQWKTRD 245
           FIH+TYLLHPI LA++LYVVGG+PF +WGM VR VVVLH + MVNS+CH  GKQ W T D
Sbjct: 187 FIHQTYLLHPIVLAVILYVVGGIPFLVWGMAVRIVVVLHFTLMVNSVCHIWGKQSWTTGD 246

Query: 246 LSINNGWMSLVTFGDSWHNNHHAFPYSAQHGFEWWQIDFSWYLIVFLQAIGVATNVKLP 300
           +S NN W+SL+TFG+ WHNNHHAF YSA+ G EWWQ+DF WY+I+FLQAIGVAT+VKLP
Sbjct: 247 MSKNNWWVSLITFGEGWHNNHHAFEYSAKFGLEWWQLDFGWYMILFLQAIGVATHVKLP 305

BLAST of HG10007851 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 337.4 bits (864), Expect = 1.7e-91
Identity = 156/291 (53.61%), Postives = 200/291 (68.73%), Query Frame = 0

Query: 11  EKSPTSFGKEEWNEIDKEMAFIIFFLHVLCIFAPFHFNWSALRVAMVLYVITGLFGISLS 70
           +K    + + EW  +D     ++  +H+L + APF FNW A+ VA  LY++TGL GI+LS
Sbjct: 87  KKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLS 146

Query: 71  YHRNLSHKSFKLPKWLEYLFAYCGVQALEGNPIDWVSMHRYHHQVADTEKDPQRPDRKFL 130
           +HRNLSHK+FKLPKWLEYLFAYCG QAL+GNPIDWVS HRYHHQ  D+++DP  P   F 
Sbjct: 147 FHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGF- 206

Query: 131 WLS--NYYASTKIVNLRYFVIFQEIEKNIFLMVRKQGRRDNVGDLEKQMFYKFIHKTYLL 190
           W S  N+   T  +  R                   G  +NVGDLEKQ FY+F+  TY+L
Sbjct: 207 WFSHMNWMFDTNTITQRC------------------GEPNNVGDLEKQPFYRFLRTTYIL 266

Query: 191 HPIALAILLYVVGGMPFFIWGMCVRTVVVLHTSFMVNSICHRSGKQQWKTRDLSINNGWM 250
           HP+ALA+ LY +GG PF +WGM VR V V H +++VNS CH  GKQ W T DLS NN W+
Sbjct: 267 HPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWV 326

Query: 251 SLVTFGDSWHNNHHAFPYSAQHGFEWWQIDFSWYLIVFLQAIGVATNVKLP 300
           + + FG+ WHNNHHAF +SA+HG EWWQ+D +WY++ FLQAIG+AT+VKLP
Sbjct: 327 AALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLP 358

BLAST of HG10007851 vs. ExPASy Swiss-Prot
Match: O65797 (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 1.7e-80
Identity = 140/297 (47.14%), Postives = 196/297 (65.99%), Query Frame = 0

Query: 3   VSIEPREVEKSPTSFGKEEWNEIDKEMAFIIFFLHVLCIFAPFHFNWSALRVAMVLYVIT 62
           ++ +  E+ +   +  + +W  +D   AF   F+H LC+ APF+F W ALRVA+++Y + 
Sbjct: 15  MAADKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVG 74

Query: 63  GLFGISLSYHRNLSHKSFKLPKWLEYLFAYCGVQALEGNPIDWVSMHRYHHQVADTEKDP 122
           GL GI++SYHRNL+H+SFK+PKWLEY FAYCG+ A++G+PIDWVS HRYHHQ  D+++DP
Sbjct: 75  GL-GITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDP 134

Query: 123 QRPDRKFLWLSNYYASTKIVNLRYFVIFQEIEKNIFLMVRKQGRRDNVGDLEKQMFYKFI 182
             P+  F W S+         L  F        +   +V K GRR NV DL++Q +YKF+
Sbjct: 135 HSPNEGF-WFSHL--------LWLF--------DTGYLVEKCGRRTNVEDLKRQWYYKFL 194

Query: 183 HKTYLLHPIALAILLYVVGGMPFFIWGMCVRTVVVLHTSFMVNSICHRSGKQQWKTRDLS 242
            +T L H +    LLY  GG+ F  WGM +   +  H + ++NS+CH  G + WKT D S
Sbjct: 195 QRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTS 254

Query: 243 INNGWMSLVTFGDSWHNNHHAFPYSAQHGFEWWQIDFSWYLIVFLQAIGVATNVKLP 300
            N  W+S+ +FG+SWHNNHHAF  SA+ G EWWQID SWY++ FL+ IG+AT+VKLP
Sbjct: 255 RNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLP 293

BLAST of HG10007851 vs. ExPASy Swiss-Prot
Match: P0DOW3 (Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 7.3e-79
Identity = 137/278 (49.28%), Postives = 189/278 (67.99%), Query Frame = 0

Query: 22  WNEIDKEMAFIIFFLHVLCIFAPFHFNWSALRVAMVLYVITGLFGISLSYHRNLSHKSFK 81
           WN  D     ++  LHVLC+ APF F+WSA  +++ LY + G+FG +LSYHRNL+H+SFK
Sbjct: 37  WNSTDIFHIILVGGLHVLCLSAPFTFSWSAFWLSLTLYAVCGVFGTTLSYHRNLTHRSFK 96

Query: 82  LPKWLEYLFAYCGVQALEGNPIDWVSMHRYHHQVADTEKDPQRPDRKFLWLSNYYASTKI 141
           LPK+LEY FAY G+ AL+G+P+ WVS HRYHH+  DT  DP  P   F W S+ +    +
Sbjct: 97  LPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKFTDTYLDPHSPIEGF-WFSHIF---WL 156

Query: 142 VNLRYFVIFQEIEKNIFLMVRKQGRRDNVGDLEKQMFYKFIHKTYLLHPIALAILLYVVG 201
            + +Y             ++ + GR +N GDL KQ +Y+F+ +T++ H    A LLY+ G
Sbjct: 157 FDSKY-------------ILEECGRYENAGDLLKQSYYRFLERTFVFHVYLQAALLYMFG 216

Query: 202 GMPFFIWGMCVRTVVVLHTSFMVNSICHRSGKQQWKTRDLSINNGWMSLVTFGDSWHNNH 261
           G PF +WGM VRTV   H S++VNS+CHR G Q W T DLS NN +++++T G+ WHNNH
Sbjct: 217 GFPFIVWGMAVRTVWGFHASWLVNSVCHRYGHQAWDTGDLSTNNWFIAMLTSGEGWHNNH 276

Query: 262 HAFPYSAQHGFEWWQIDFSWYLIVFLQAIGVATNVKLP 300
           HAF YSA+HG EWWQID +WY+I  L+ +G+AT+VK+P
Sbjct: 277 HAFEYSARHGIEWWQIDTTWYVIKLLEYLGLATDVKVP 297

BLAST of HG10007851 vs. ExPASy Swiss-Prot
Match: Q9FV68 (Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 3.6e-78
Identity = 142/301 (47.18%), Postives = 198/301 (65.78%), Query Frame = 0

Query: 10  VEKSPTSFGKEEWNEIDKEMAFIIFFLHVLCIFAPFHFNWSALRVAMVLY-VITGLFGIS 69
           +E+    F + +WN  D + A  +  LH L   APF+F+W A  ++ +L    +G+ GI+
Sbjct: 69  LERKAKGFWRRKWNPRDIQNAVTLLVLHALAAMAPFYFSWDAFWISFILLGFASGVLGIT 128

Query: 70  LSYHRNLSHKSFKLPKWLEYLFAYCGVQALEGNPIDWVSMHRYHHQVADTEKDPQRPDRK 129
           L +HR L+H  FKLPK +EY FAYCG  AL+G+P++WVS HRYHHQ  DTE+D   P + 
Sbjct: 129 LCFHRCLTHGGFKLPKLVEYFFAYCGSLALQGDPMEWVSNHRYHHQFVDTERDVHSPTQG 188

Query: 130 FLWLSNYYASTKIVNLRYFVIFQEIEKNIFLMVRKQGRRDNVGDLEKQMFYKFIHKTYLL 189
           F W  +              I   ++K++F + ++ GRR+NV DL+KQ FY+F+ KTY+ 
Sbjct: 189 F-WFCH--------------IGWVLDKDLF-VEKRGGRRNNVNDLKKQAFYRFLQKTYMY 248

Query: 190 HPIALAILLYVVGGMPFFIWGMCVRTVVVLHTSFMVNSICHRSGKQQWKTRDLSINNGWM 249
           H +AL  LLY VGG P+ +WGM  R V + H++F +NS+CH+ G + W T DLS NN ++
Sbjct: 249 HQLALIALLYYVGGFPYIVWGMGFRLVFMFHSTFAINSVCHKWGGRPWNTGDLSTNNMFV 308

Query: 250 SLVTFGDSWHNNHHAFPYSAQHGFEWWQIDFSWYLIVFLQAIGVATNVKLPPHKATLGKL 309
           +L  FG+ WHNNHHAF  SA+HG EWW+ID +WY+I  LQAIG+ATNVKLP  +A   KL
Sbjct: 309 ALCAFGEGWHNNHHAFEQSARHGLEWWEIDVTWYVIRTLQAIGLATNVKLPT-EAQKQKL 352

BLAST of HG10007851 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 6.2e-78
Identity = 136/289 (47.06%), Postives = 189/289 (65.40%), Query Frame = 0

Query: 11  EKSPTSFGKEEWNEIDKEMAFIIFFLHVLCIFAPFHFNWSALRVAMVLYVITGLFGISLS 70
           EK+       +WN  D      I  LHVLC+FAP  F+W +  V   LY I G+FG +LS
Sbjct: 29  EKTKKITWVRKWNSTDVFHILFIGGLHVLCLFAPSTFSWKSFWVCFALYAICGVFGTTLS 88

Query: 71  YHRNLSHKSFKLPKWLEYLFAYCGVQALEGNPIDWVSMHRYHHQVADTEKDPQRPDRKFL 130
           +HRNL+H+SFKLPK+LEY FAY G+ AL+G+P+ WVS HRYHH+  DT  DP  P   F 
Sbjct: 89  FHRNLTHRSFKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKYTDTYLDPHSPIEGF- 148

Query: 131 WLSNYYASTKIVNLRYFVIFQEIEKNIFLMVRKQGRRDNVGDLEKQMFYKFIHKTYLLHP 190
           W  + +    + + +Y             ++ K GR +N GDL KQ +Y+F+ +T++ H 
Sbjct: 149 WFCHIF---WLFDSKY-------------IIEKCGRYENAGDLMKQSYYRFLERTFVYHV 208

Query: 191 IALAILLYVVGGMPFFIWGMCVRTVVVLHTSFMVNSICHRSGKQQWKTRDLSINNGWMSL 250
              A LLY+ GG PF +WGM VRT++  H S++VNS+CHR G + W T DLS NN ++++
Sbjct: 209 YLQAALLYLFGGFPFIVWGMAVRTILGFHLSWLVNSVCHRWGNRPWNTGDLSTNNWFIAM 268

Query: 251 VTFGDSWHNNHHAFPYSAQHGFEWWQIDFSWYLIVFLQAIGVATNVKLP 300
           +T G+ WHNNHHAF YSA+HG EWWQID +WY+I  L+ +G+AT++K+P
Sbjct: 269 LTSGEGWHNNHHAFEYSARHGIEWWQIDTTWYIIKLLEYLGLATDIKVP 300

BLAST of HG10007851 vs. ExPASy TrEMBL
Match: A0A6J1JGI0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111485493 PE=3 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 2.1e-129
Identity = 223/300 (74.33%), Postives = 247/300 (82.33%), Query Frame = 0

Query: 1   MDVSIEPREVEKSPTSFGKEEWNEIDKEMAFIIFFLHVLCIFAPFHFNWSALRVAMVLYV 60
           MDV IEP +  K P S GKEEW +IDK+ A  IF +HVLCIFAPF  N  A+RVA+ L+ 
Sbjct: 1   MDVPIEPMDTAKPPRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRAIRVALALHF 60

Query: 61  ITGLFGISLSYHRNLSHKSFKLPKWLEYLFAYCGVQALEGNPIDWVSMHRYHHQVADTEK 120
           ITG FGI+LSYHRNLSH+SFKLPKW EYLFA CGV  L G+PI+WVSMHRYHHQVADTEK
Sbjct: 61  ITGFFGITLSYHRNLSHRSFKLPKWFEYLFALCGVHTLLGDPINWVSMHRYHHQVADTEK 120

Query: 121 DPQRPDRKFLWLSNYYASTKIVNLRYFVIFQEIEKNIFLMVRKQGRRDNVGDLEKQMFYK 180
           DPQR  R+FLWLSNY+ASTK V  RY  I Q IEKNIFLM+RK  +RDNVGDLE Q FYK
Sbjct: 121 DPQRTPRRFLWLSNYFASTKRVGPRYLCILQGIEKNIFLMIRKFVKRDNVGDLENQSFYK 180

Query: 181 FIHKTYLLHPIALAILLYVVGGMPFFIWGMCVRTVVVLHTSFMVNSICHRSGKQQWKTRD 240
           FIHKTYLLHPI LAILLYVVGGMPF +WGM VRTV+VLH S MVN+ICHR GK+ WKT D
Sbjct: 181 FIHKTYLLHPIGLAILLYVVGGMPFLVWGMGVRTVLVLHLSLMVNTICHRFGKRSWKTND 240

Query: 241 LSINNGWMSLVTFGDSWHNNHHAFPYSAQHGFEWWQIDFSWYLIVFLQAIGVATNVKLPP 300
            S NNG M L++FG+ WHNNHHAFPYSA+HGFEWWQID SWY I FLQAIGVAT+VKLPP
Sbjct: 241 SSRNNGLMGLISFGEGWHNNHHAFPYSARHGFEWWQIDTSWYAIRFLQAIGVATDVKLPP 300

BLAST of HG10007851 vs. ExPASy TrEMBL
Match: A0A6J1H9D0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111461702 PE=3 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 7.0e-125
Identity = 214/289 (74.05%), Postives = 240/289 (83.04%), Query Frame = 0

Query: 12  KSPTSFGKEEWNEIDKEMAFIIFFLHVLCIFAPFHFNWSALRVAMVLYVITGLFGISLSY 71
           K   S GKEEW +IDK+ A  IF +HVLCIFAPF  N   LR+A+ L+ ITG FGI+LSY
Sbjct: 5   KPRRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRVLRIALALHFITGFFGITLSY 64

Query: 72  HRNLSHKSFKLPKWLEYLFAYCGVQALEGNPIDWVSMHRYHHQVADTEKDPQRPDRKFLW 131
           HRNLSH+SFKLPKWLEYLFA CG   L G+PI+WVSMHRYHHQVADTEKDPQR  R+FLW
Sbjct: 65  HRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWVSMHRYHHQVADTEKDPQRTPRRFLW 124

Query: 132 LSNYYASTKIVNLRYFVIFQEIEKNIFLMVRKQGRRDNVGDLEKQMFYKFIHKTYLLHPI 191
           LSNY+ASTK V  RY  I + IEKNIFLM+R+  +RDNVGDLE Q FYKFIHKTYLLHPI
Sbjct: 125 LSNYFASTKRVGPRYLFILEGIEKNIFLMIREFVKRDNVGDLENQSFYKFIHKTYLLHPI 184

Query: 192 ALAILLYVVGGMPFFIWGMCVRTVVVLHTSFMVNSICHRSGKQQWKTRDLSINNGWMSLV 251
            LAILLYVVGGMPF +WGM VRTV+VLH S MVN+ICHR GK+QWKT D S NNG MSL+
Sbjct: 185 GLAILLYVVGGMPFLVWGMGVRTVLVLHLSLMVNTICHRFGKRQWKTNDSSRNNGLMSLI 244

Query: 252 TFGDSWHNNHHAFPYSAQHGFEWWQIDFSWYLIVFLQAIGVATNVKLPP 301
           +FG+ WHNNHHAFPYSA+HGFEWWQ+D SWY I FLQAIGVAT+VKLPP
Sbjct: 245 SFGEGWHNNHHAFPYSARHGFEWWQVDTSWYAIRFLQAIGVATDVKLPP 293

BLAST of HG10007851 vs. ExPASy TrEMBL
Match: A0A6J1DJP0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111021101 PE=3 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 1.9e-114
Identity = 191/299 (63.88%), Postives = 238/299 (79.60%), Query Frame = 0

Query: 6   EPREVEKSPTSFGKEEWNEIDKEMAFIIFFLHVLCIFAPFHFNWSALRVAMVLYVITGLF 65
           EP+E+ K P  + + +W ++DK++A  +F +H+LCI APF FNWSA  VA VLY++TGLF
Sbjct: 7   EPKEMMKPPRPYERRKWTKLDKDIACSLFLVHLLCILAPFQFNWSAFWVAFVLYIVTGLF 66

Query: 66  GISLSYHRNLSHKSFKLPKWLEYLFAYCGVQALEGNPIDWVSMHRYHHQVADTEKDPQRP 125
           G++LSYHRNL+HKSFKLPKWLEYLFAYCGVQAL+G+P+DWVS HR HHQ  DTE+DP  P
Sbjct: 67  GMTLSYHRNLAHKSFKLPKWLEYLFAYCGVQALQGDPMDWVSTHRCHHQFVDTERDPHSP 126

Query: 126 DRKF-----LWLSNYYASTKIVNLRYFVIFQEIEKNIFLMVRKQGRRDNVGDLEKQMFYK 185
            R F     LWL + Y  TK V  +YF  FQ++E+NIF++  K G+ +NV DLEKQ FY+
Sbjct: 127 IRGFWFSHILWLFDSYNLTKKVCPKYFSNFQKLERNIFVLFSKHGKPENVADLEKQAFYR 186

Query: 186 FIHKTYLLHPIALAILLYVVGGMPFFIWGMCVRTVVVLHTSFMVNSICHRSGKQQWKTRD 245
           FIH+TYLLHPI LA++LYVVGG+PF +WGM VR VVVLH + MVNS+CH  GKQ W T D
Sbjct: 187 FIHQTYLLHPIVLAVILYVVGGIPFLVWGMAVRIVVVLHFTLMVNSVCHIWGKQSWTTGD 246

Query: 246 LSINNGWMSLVTFGDSWHNNHHAFPYSAQHGFEWWQIDFSWYLIVFLQAIGVATNVKLP 300
           +S NN W+SL+TFG+ WHNNHHAF YSA+ G EWWQ+DF WY+I+FLQAIGVAT+VKLP
Sbjct: 247 MSKNNWWVSLITFGEGWHNNHHAFEYSAKFGLEWWQLDFGWYMILFLQAIGVATHVKLP 305

BLAST of HG10007851 vs. ExPASy TrEMBL
Match: A0A6J1DHZ7 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111021091 PE=3 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 5.2e-112
Identity = 190/300 (63.33%), Postives = 235/300 (78.33%), Query Frame = 0

Query: 6   EPREVEKSPTSFGK-EEWNEIDKEMAFIIFFLHVLCIFAPFHFNWSALRVAMVLYVITGL 65
           EP+E+   P SFGK ++W ++DK++A  +FF+H+L +FAPF+FNW A  +A  LYV TGL
Sbjct: 27  EPKEMAIPPKSFGKRKKWTKLDKDVACSLFFVHLLSMFAPFYFNWRAFWIAFALYVATGL 86

Query: 66  FGISLSYHRNLSHKSFKLPKWLEYLFAYCGVQALEGNPIDWVSMHRYHHQVADTEKDPQR 125
           FGIS+SYHRNL+HKSF LPKWLEYLFAYCG  AL+G+PIDWV+ HR HH+  DTE+DP  
Sbjct: 87  FGISVSYHRNLAHKSFTLPKWLEYLFAYCGAHALQGDPIDWVNTHRCHHRFVDTERDPHS 146

Query: 126 PDRKF-----LWLSNYYASTKIVNLRYFVIFQEIEKNIFLMVRKQGRRDNVGDLEKQMFY 185
           P + F      WL + Y+ TK V  +YF  FQ++E+NIF+M  K GR DNVGDLEKQ FY
Sbjct: 147 PIQGFWFSHITWLLDSYSLTKRVCPKYFTDFQKVERNIFVMFSKHGRPDNVGDLEKQTFY 206

Query: 186 KFIHKTYLLHPIALAILLYVVGGMPFFIWGMCVRTVVVLHTSFMVNSICHRSGKQQWKTR 245
           +FIHKTY LHPI LAI+LY  GG PF IWGM VR VVVLH + M+NS+CH  GKQ W+T 
Sbjct: 207 RFIHKTYFLHPIILAIILYAYGGAPFLIWGMGVRIVVVLHITCMINSVCHIWGKQPWRTG 266

Query: 246 DLSINNGWMSLVTFGDSWHNNHHAFPYSAQHGFEWWQIDFSWYLIVFLQAIGVATNVKLP 300
           DLS NN W+SLV+FG+ WHNNHHAF YSA+HG EWWQIDF WY+++FL+AIG+ATNVK+P
Sbjct: 267 DLSRNNWWVSLVSFGEGWHNNHHAFEYSARHGIEWWQIDFGWYVVMFLEAIGIATNVKVP 326

BLAST of HG10007851 vs. ExPASy TrEMBL
Match: A0A6J1CTB0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111014139 PE=3 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 5.8e-111
Identity = 191/292 (65.41%), Postives = 228/292 (78.08%), Query Frame = 0

Query: 19  KEEWNEIDKEMAFIIFFLHVLCIFAPFHFNWSALRVAMVLYVITGLFGISLSYHRNLSHK 78
           K EW +ID +MA I FF+HV CIFAPFHFNW A  VA  LY ITGLFG+S+SYHRNLSHK
Sbjct: 3   KGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSISYHRNLSHK 62

Query: 79  SFKLPKWLEYLFAYCGVQALEGNPIDWVSMHRYHHQVADTEKDPQRPDRKFL-----WLS 138
           SFKLPKW EYL AYCG+ AL+G+PIDWVS HRYHHQ  DTE+DP  P +  L     W  
Sbjct: 63  SFKLPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPDSPTQGLLFSYITWAF 122

Query: 139 NYYASTKIVNLRYFVIFQEIEKNIFLMVRKQGRRDNVGDLEKQMFYKFIHKTYLLHPIAL 198
           + YA TK V  +YF  F+E E+ IF+M RK GR +NVGDLEKQ FY+FIHKTYLLH IAL
Sbjct: 123 DSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKTYLLHVIAL 182

Query: 199 AILLYVVGGMPFFIWGMCVRTVVVLHTSFMVNSICHRSGKQQWKTRDLSINNGWMSLVTF 258
           A++LYVVGG+PF IWG  VRTV++LH++FMV+SICH+ G QQW    LS NN WM+ ++F
Sbjct: 183 AVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNNWWMTFISF 242

Query: 259 GDSWHNNHHAFPYSAQHGFEWWQIDFSWYLIVFLQAIGVATNVKLPPHKATL 306
           G++WHNNHHAF YSA+ GFEWWQIDF WY+I+FL+ IGVAT+VKLP    T+
Sbjct: 243 GENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLPRMSLTI 294

BLAST of HG10007851 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 337.4 bits (864), Expect = 1.2e-92
Identity = 156/291 (53.61%), Postives = 200/291 (68.73%), Query Frame = 0

Query: 11  EKSPTSFGKEEWNEIDKEMAFIIFFLHVLCIFAPFHFNWSALRVAMVLYVITGLFGISLS 70
           +K    + + EW  +D     ++  +H+L + APF FNW A+ VA  LY++TGL GI+LS
Sbjct: 87  KKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLS 146

Query: 71  YHRNLSHKSFKLPKWLEYLFAYCGVQALEGNPIDWVSMHRYHHQVADTEKDPQRPDRKFL 130
           +HRNLSHK+FKLPKWLEYLFAYCG QAL+GNPIDWVS HRYHHQ  D+++DP  P   F 
Sbjct: 147 FHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGF- 206

Query: 131 WLS--NYYASTKIVNLRYFVIFQEIEKNIFLMVRKQGRRDNVGDLEKQMFYKFIHKTYLL 190
           W S  N+   T  +  R                   G  +NVGDLEKQ FY+F+  TY+L
Sbjct: 207 WFSHMNWMFDTNTITQRC------------------GEPNNVGDLEKQPFYRFLRTTYIL 266

Query: 191 HPIALAILLYVVGGMPFFIWGMCVRTVVVLHTSFMVNSICHRSGKQQWKTRDLSINNGWM 250
           HP+ALA+ LY +GG PF +WGM VR V V H +++VNS CH  GKQ W T DLS NN W+
Sbjct: 267 HPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWV 326

Query: 251 SLVTFGDSWHNNHHAFPYSAQHGFEWWQIDFSWYLIVFLQAIGVATNVKLP 300
           + + FG+ WHNNHHAF +SA+HG EWWQ+D +WY++ FLQAIG+AT+VKLP
Sbjct: 327 AALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLP 358

BLAST of HG10007851 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 300.8 bits (769), Expect = 1.2e-81
Identity = 140/297 (47.14%), Postives = 196/297 (65.99%), Query Frame = 0

Query: 3   VSIEPREVEKSPTSFGKEEWNEIDKEMAFIIFFLHVLCIFAPFHFNWSALRVAMVLYVIT 62
           ++ +  E+ +   +  + +W  +D   AF   F+H LC+ APF+F W ALRVA+++Y + 
Sbjct: 15  MAADKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVG 74

Query: 63  GLFGISLSYHRNLSHKSFKLPKWLEYLFAYCGVQALEGNPIDWVSMHRYHHQVADTEKDP 122
           GL GI++SYHRNL+H+SFK+PKWLEY FAYCG+ A++G+PIDWVS HRYHHQ  D+++DP
Sbjct: 75  GL-GITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDP 134

Query: 123 QRPDRKFLWLSNYYASTKIVNLRYFVIFQEIEKNIFLMVRKQGRRDNVGDLEKQMFYKFI 182
             P+  F W S+         L  F        +   +V K GRR NV DL++Q +YKF+
Sbjct: 135 HSPNEGF-WFSHL--------LWLF--------DTGYLVEKCGRRTNVEDLKRQWYYKFL 194

Query: 183 HKTYLLHPIALAILLYVVGGMPFFIWGMCVRTVVVLHTSFMVNSICHRSGKQQWKTRDLS 242
            +T L H +    LLY  GG+ F  WGM +   +  H + ++NS+CH  G + WKT D S
Sbjct: 195 QRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTS 254

Query: 243 INNGWMSLVTFGDSWHNNHHAFPYSAQHGFEWWQIDFSWYLIVFLQAIGVATNVKLP 300
            N  W+S+ +FG+SWHNNHHAF  SA+ G EWWQID SWY++ FL+ IG+AT+VKLP
Sbjct: 255 RNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLP 293

BLAST of HG10007851 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 285.4 bits (729), Expect = 5.4e-77
Identity = 139/291 (47.77%), Postives = 184/291 (63.23%), Query Frame = 0

Query: 9   EVEKSPTSFGKEEWNEIDKEMAFIIFFLHVLCIFAPFHFNWSALRVAMVLYVITGLFGIS 68
           E +K    F    W  +D       F +H L + APF+F WSAL V  + Y I GL GI+
Sbjct: 23  EKKKRRWVFWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGL-GIT 82

Query: 69  LSYHRNLSHKSFKLPKWLEYLFAYCGVQALEGNPIDWVSMHRYHHQVADTEKDPQRPDRK 128
           +SYHRNL+H+SFK+PKWLEYL AYC + A++G+PIDWVS HRYHHQ  D+E+DP  P   
Sbjct: 83  VSYHRNLAHRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEG 142

Query: 129 FLWLSNYYASTKIVNLRYFVIFQEIEKNIFLMVRKQGRRDNVGDLEKQMFYKFIHKTYLL 188
           F W S+      I +  Y             +V K GRR NV DL++Q FY+F+ KT L 
Sbjct: 143 F-WFSHL---LWIYDSAY-------------LVSKCGRRANVEDLKRQWFYRFLQKTVLF 202

Query: 189 HPIALAILLYVVGGMPFFIWGMCVRTVVVLHTSFMVNSICHRSGKQQWKTRDLSINNGWM 248
           H + L   L+ +GGM F  WGM V   + +H + ++NS+CH  G + WKT D S N  W+
Sbjct: 203 HILGLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWL 262

Query: 249 SLVTFGDSWHNNHHAFPYSAQHGFEWWQIDFSWYLIVFLQAIGVATNVKLP 300
           S+ +FG+SWHNNHHAF  SA+ G EWWQID SWY++ F + IG+AT+VK+P
Sbjct: 263 SVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKVP 295

BLAST of HG10007851 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 282.0 bits (720), Expect = 6.0e-76
Identity = 139/298 (46.64%), Postives = 186/298 (62.42%), Query Frame = 0

Query: 10  VEKSPTSFGKEEWNEIDKEMAFIIFFLHVLCIFAPFHFNWSALRVAMVLYVITGLFGISL 69
           V ++  SF +  W   D     I    H+L + APF+F+W A  V   L  I G+  I+L
Sbjct: 79  VVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGIC-ITL 138

Query: 70  SYHRNLSHKSFKLPKWLEYLFAYCGVQALEGNPIDWVSMHRYHHQVADTEKDPQRPDRKF 129
           SYHRNLSH+SF LPKWLEYLFAY GV A +G+PI+WVS HRYHH+  +T++DP  P + F
Sbjct: 139 SYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGF 198

Query: 130 LWLSNYYASTKIVNLRYFVIFQEIEKNIFLMVRKQGRRDNVGDLEKQMFYKFIHKTYLLH 189
            W S         ++ +      I +N        G  +NV DL +Q FY+F+ +T LLH
Sbjct: 199 -WFS---------HMAWIFDTSSILENC-------GGEENVDDLVRQPFYRFLQRTVLLH 258

Query: 190 PIALAILLYVVGGMPFFIWGMCVRTVVVLHTSFMVNSICHRSGKQQWKTRDLSINNGWMS 249
            +A + L Y  GGMP  +WG+ +   V LH +F+VNS+CH  G + W T D S NN W++
Sbjct: 259 MMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFSKNNWWVA 318

Query: 250 LVTFGDSWHNNHHAFPYSAQHGFEWWQIDFSWYLIVFLQAIGVATNVKLPPHKATLGK 308
           ++T G+ WHNNHHAF +SA+HG EWWQ+D +W LI FL+AIG+ATNVKLP      GK
Sbjct: 319 ILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTETQMKGK 358

BLAST of HG10007851 vs. TAIR 10
Match: AT1G06100.1 (Fatty acid desaturase family protein )

HSP 1 Score: 280.0 bits (715), Expect = 2.3e-75
Identity = 134/298 (44.97%), Postives = 191/298 (64.09%), Query Frame = 0

Query: 2   DVSIEPREVEKSPTSFGKEEWNEIDKEMAFIIFFLHVLCIFAPFHFNWSALRVAMVLYVI 61
           D S + + V K   ++   +W + D   A  +  +H+LC+ APF++ W A R  ++L ++
Sbjct: 8   DGSSQKKSVRKEKRAYVLRKWTQFDVGRASTVGTVHLLCLLAPFNYKWEAFRFGIILAIL 67

Query: 62  TGLFGISLSYHRNLSHKSFKLPKWLEYLFAYCGVQALEGNPIDWVSMHRYHHQVADTEKD 121
           T L  I+ SYHRNL+H+SFKLPKWLEY FAY  + AL+G+P+DWVS+HR+HHQ  D+++D
Sbjct: 68  TNLC-ITFSYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDWVSIHRFHHQFTDSDRD 127

Query: 122 PQRPDRKFLWLSNYYASTKIVNLRYFVIFQEIEKNIFLMVRKQGRRDNVGDLEKQMFYKF 181
           P  P   F W S+         +R                 K GRR+NV DL++Q FY+F
Sbjct: 128 PHSPIEGF-WFSHVLWIFDTDYIR----------------EKCGRRNNVMDLKQQWFYRF 187

Query: 182 IHKTYLLHPIALAILLYVVGGMPFFIWGMCVRTVVVLHTSFMVNSICHRSGKQQWKTRDL 241
           + KT +LH +A   L+Y+ GG+P+  W +    V+  H +++VNS CH  G Q W+T D 
Sbjct: 188 LKKTLVLHILAFWTLIYLWGGLPYLTWTVGFGGVIGYHGTWLVNSACHICGSQAWQTNDT 247

Query: 242 SINNGWMSLVTFGDSWHNNHHAFPYSAQHGFEWWQIDFSWYLIVFLQAIGVATNVKLP 300
           S N  W++L+T G+SWHNNHHAF  SA+HG EW+Q+D +WYLI F QA+G+ATNVKLP
Sbjct: 248 SRNVWWLALLTMGESWHNNHHAFETSARHGLEWYQLDITWYLIWFFQALGLATNVKLP 287

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038879870.12.5e-14884.21palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [B... [more]
XP_022988176.14.4e-12974.33palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C... [more]
XP_022961086.11.5e-12474.05palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C... [more]
XP_023533044.11.2e-12371.14acyl-CoA C20 Delta5-desaturase-like [Cucurbita pepo subsp. pepo][more]
XP_022153644.14.0e-11463.88palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M... [more]
Match NameE-valueIdentityDescription
Q949X01.7e-9153.61Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab... [more]
O657971.7e-8047.14Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=... [more]
P0DOW37.3e-7949.28Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1[more]
Q9FV683.6e-7847.18Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1[more]
P0DOW26.2e-7847.06Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JGI02.1e-12974.33palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1H9D07.0e-12574.05palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1DJP01.9e-11463.88palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1DHZ75.2e-11263.33palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1CTB05.8e-11165.41palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
Match NameE-valueIdentityDescription
AT3G15850.11.2e-9253.61fatty acid desaturase 5 [more]
AT1G06080.11.2e-8147.14delta 9 desaturase 1 [more]
AT2G31360.15.4e-7747.7716:0delta9 desaturase 2 [more]
AT3G15870.16.0e-7646.64Fatty acid desaturase family protein [more]
AT1G06100.12.3e-7544.97Fatty acid desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015876Acyl-CoA desaturasePRINTSPR00075FACDDSATRASEcoord: 109..138
score: 31.23
coord: 254..268
score: 53.68
coord: 72..92
score: 32.83
IPR015876Acyl-CoA desaturasePANTHERPTHR11351ACYL-COA DESATURASEcoord: 19..300
IPR015876Acyl-CoA desaturaseCDDcd03505Delta9-FADS-likecoord: 48..297
e-value: 6.57601E-61
score: 190.074
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 55..278
e-value: 1.3E-18
score: 67.8
NoneNo IPR availablePANTHERPTHR11351:SF94BNAC05G37460D PROTEINcoord: 19..300

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10007851.1HG10007851.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0009979 16:0 monogalactosyldiacylglycerol desaturase activity
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water